Restriction map for
pF1KE2392
(Circular) (Six-base) MAPSORT of: pF1KE2392.seq Check: 135 from: 1 to: 4664
pF1KE2392 4664 bp
With 182 enzymes: SgfI *
February 7, 2012 15:11 ..
AccI GT'mk_AC
Cuts at: 1702 1702
Size: 4664
Acc65I G'GTAC_C
Cuts at: 1680 1680
Size: 4664
AclI AA'CG_TT
Cuts at: 4504 4504
Size: 4664
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 1420 2489 2921 3668 3936 292
Size: 1128 1069 432 747 268 1020
Fragments arranged by size:
1128 1069 1020 747 432 268
AfeI AGC'GCT
Cuts at: 1343 1343
Size: 4664
AflIII A'CryG_T
Cuts at: 2016 3120 4097 2016
Size: 1104 977 2583
Fragments arranged by size:
2583 1104 977
AgeI A'CCGG_T
Cuts at: 3052 3052
Size: 4664
AhdI GACnn_n'nnGTC
Cuts at: 1218 1218
Size: 4664
AleI CACnn'nnGTG
Cuts at: 424 424
Size: 4664
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1289 1321 1289
Size: 32 4632
AlwNI CAG_nnn'CTG
Cuts at: 1170 1969 3536 1170
Size: 799 1567 2298
Fragments arranged by size:
2298 1567 799
ApaI G_GGCC'C
Cuts at: 168 168
Size: 4664
ApaLI G'TGCA_C
Cuts at: 3434 3434
Size: 4664
ApoI r'AATT_y
Cuts at: 1668 4017 4273 1668
Size: 2349 256 2059
Fragments arranged by size:
2349 2059 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4664
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4664
AvaI C'yCGr_G
Cuts at: 1127 1242 1684 1127
Size: 115 442 4107
Fragments arranged by size:
4107 442 115
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 501 534 501
Size: 33 4631
BamHI G'GATC_C
Cuts at: 375 1689 375
Size: 1314 3350
BanI G'GyrC_C
Cuts at: 411 768 990 1011 1463 1605 1680 2094 2342
Size: 357 222 21 452 142 75 414 248
Cuts at: 2342 2377 411
Size: 35 2698
Fragments arranged by size:
2698 452 414 357 248 222 142 75
35 21
BanII G_rGCy'C
Cuts at: 168 1678 2708 168
Size: 1510 1030 2124
Fragments arranged by size:
2124 1510 1030
BbeI G_GCGC'C
Cuts at: 772 2098 2346 772
Size: 1326 248 3090
Fragments arranged by size:
3090 1326 248
BbsI GAAGACnn'nnnn_
Cuts at: 462 957 462
Size: 495 4169
BbvCI CC'TCA_GC
Cuts at: 406 1171 406
Size: 765 3899
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 480 514 90
Size: 34 356 34 4240
Fragments arranged by size:
4240 356 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2562 3323 4664 2562
Size: 761 1341 2562
Fragments arranged by size:
2562 1341 761
BclI T'GATC_A
Cuts at: 2184 2184
Size: 4664
BfrBI ATG'CAT
Cuts at: 111 111
Size: 4664
BglI GCCn_nnn'nGGC
Cuts at: 1423 1423
Size: 4664
BglII A'GATC_T
Cuts at: 2179 4216 2179
Size: 2037 2627
BlpI GC'TnA_GC
Cuts at: 1378 1776 1378
Size: 398 4266
Bme1580I G_kGCm'C
Cuts at: 168 1461 2289 2382 3438 168
Size: 1293 828 93 1056 1394
Fragments arranged by size:
1394 1293 1056 828 93
BmrI ACTGGGnnnn_n'
Cuts at: 1212 1271 1876 1957 2292 4436 1212
Size: 59 605 81 335 2144 1440
Fragments arranged by size:
2144 1440 605 335 81 59
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3031 3031
Size: 4664
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 1366 1646 469
Size: 897 280 3487
Fragments arranged by size:
3487 897 280
Bpu10I CC'TnA_GC
Cuts at: 406 1171 406
Size: 765 3899
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1840 2719 3211 3509 1840
Size: 879 492 298 2995
Fragments arranged by size:
2995 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1109 4368 1109
Size: 3259 1405
BsaAI yAC'GTr
Cuts at: 2017 2017
Size: 4664
BsaBI GATnn'nnATC
Cuts at: 6 2178 4215 6
Size: 2172 2037 455
Fragments arranged by size:
2172 2037 455
BsaHI Gr'CG_yC
Cuts at: 769 2095 2343 3045 4541 769
Size: 1326 248 702 1496 892
Fragments arranged by size:
1496 1326 892 702 248
BsaWI w'CCGG_w
Cuts at: 1853 2374 3052 3326 3473 3884 1853
Size: 521 678 274 147 411 2633
Fragments arranged by size:
2633 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 280 310 1102 1132 280
Size: 30 792 30 3812
Fragments arranged by size:
3812 792 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 124 199 864 1230 1282 1291 1510 124
Size: 75 665 366 52 9 219 3278
Fragments arranged by size:
3278 665 366 219 75 52 9
BseYI C'CCAG_C
Cuts at: 371 762 913 1163 1413 2089 2847 3424 3920
Size: 391 151 250 250 676 758 577 496
Cuts at: 3920 371
Size: 1115
Fragments arranged by size:
1115 758 676 577 496 391 250 250
151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1228 1228
Size: 4664
BsiEI CG_ry'CG
Cuts at: 104 436 2206 2252 3460 104
Size: 332 1770 46 1208 1308
Fragments arranged by size:
1770 1308 1208 332 46
BsiHKAI G_wGCw'C
Cuts at: 500 869 950 1088 1678 2456 2646 3438 500
Size: 369 81 138 590 778 190 792 1726
Fragments arranged by size:
1726 792 778 590 369 190 138 81
BsmI GAATG_Cn'
Cuts at: 1077 1077
Size: 4664
Bsp1286I G_dGCh'C
Cuts at: 168 500 869 950 1088 1461 1678 2289 2382
Size: 332 369 81 138 373 217 611 93
Cuts at: 2382 2456 2646 2708 3438 168
Size: 74 190 62 730 1394
Fragments arranged by size:
1394 730 611 373 369 332 217 190
138 93 81 74 62
BspEI T'CCGG_A
Cuts at: 3884 3884
Size: 4664
BspHI T'CATG_A
Cuts at: 3840 3840
Size: 4664
BspMI ACCTGCnnnn'nnnn_
Cuts at: 236 818 1652 1714 1928 2230 2611 236
Size: 582 834 62 214 302 381 2289
Fragments arranged by size:
2289 834 582 381 302 214 62
BsrBI CCG'CTC
Cuts at: 50 2956 3010 4494 4659 50
Size: 2906 54 1484 165 55
Fragments arranged by size:
2906 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 498 1448 2576 498
Size: 950 1128 2586
Fragments arranged by size:
2586 1128 950
BsrFI r'CCGG_y
Cuts at: 1608 2662 2843 3052 1608
Size: 1054 181 209 3220
Fragments arranged by size:
3220 1054 209 181
BssSI C'ACGA_G
Cuts at: 499 2935 3293 499
Size: 2436 358 1870
Fragments arranged by size:
2436 1870 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2041 2041
Size: 4664
BstBI TT'CG_AA
Cuts at: 3025 3025
Size: 4664
BstEII G'GTnAC_C
Cuts at: 1050 1050
Size: 4664
BstXI CCAn_nnnn'nTGG
Cuts at: 1390 1390
Size: 4664
BstYI r'GATC_y
Cuts at: 1 375 1629 1689 2156 2179 2514 2760 3772
Size: 374 1254 60 467 23 335 246 1012
Cuts at: 3772 3858 3870 4216 1
Size: 86 12 346 449
Fragments arranged by size:
1254 1012 467 449 374 346 335 246
86 60 23 12
Bsu36I CC'TnA_GG
Cuts at: 698 1150 698
Size: 452 4212
BtgI C'CryG_G
Cuts at: 236 422 1121 236
Size: 186 699 3779
Fragments arranged by size:
3779 699 186
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 837 2614 2795 2958 837
Size: 1777 181 163 2543
Fragments arranged by size:
2543 1777 181 163
BtsI GCAGTG_nn'
Cuts at: 355 1299 1318 1907 355
Size: 944 19 589 3112
Fragments arranged by size:
3112 944 589 19
DraI TTT'AAA
Cuts at: 1663 1663
Size: 4664
DrdI GACnn_nn'nnGTC
Cuts at: 2370 3228 2370
Size: 858 3806
EaeI y'GGCC_r
Cuts at: 433 666 980 1541 2249 2423 2814 2841 4186
Size: 233 314 561 708 174 391 27 1345
Cuts at: 4186 433
Size: 911
Fragments arranged by size:
1345 911 708 561 391 314 233 174
27
EagI C'GGCC_G
Cuts at: 433 2249 433
Size: 1816 2848
EarI CTCTTCn'nnn_
Cuts at: 838 976 1309 1561 2687 2897 838
Size: 138 333 252 1126 210 2605
Fragments arranged by size:
2605 1126 333 252 210 138
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 540 2872 3182 3328 4474 540
Size: 2332 310 146 1146 730
Fragments arranged by size:
2332 1146 730 310 146
EcoICRI GAG'CTC
Cuts at: 1676 1676
Size: 4664
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 469 1366 1420 1646 2489 2921 3668 3936
Size: 177 897 54 226 843 432 747 268
Cuts at: 3936 292
Size: 1020
Fragments arranged by size:
1020 897 843 747 432 268 226 177
54
EcoNI CCTnn'n_nnAGG
Cuts at: 1402 1402
Size: 4664
EcoO109I rG'GnC_Cy
Cuts at: 164 1803 164
Size: 1639 3025
EcoRI G'AATT_C
Cuts at: 1668 1668
Size: 4664
EcoRV GAT'ATC
Cuts at: 441 441
Size: 4664
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 849 2683 849
Size: 1834 2830
FspI TGC'GCA
Cuts at: 2445 2445
Size: 4664
HaeII r_GCGC'y
Cuts at: 192 772 1345 2098 2346 3368 4347 192
Size: 580 573 753 248 1022 979 509
Fragments arranged by size:
1022 979 753 580 573 509 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1204 1236 1238 1270 2506 2536 2538 2568 1204
Size: 32 2 32 1236 30 2 30 3300
Fragments arranged by size:
3300 1236 32 32 30 30 2 2
HincII GTy'rAC
Cuts at: 1703 1703
Size: 4664
KasI G'GCGC_C
Cuts at: 768 2094 2342 768
Size: 1326 248 3090
Fragments arranged by size:
3090 1326 248
KpnI G_GTAC'C
Cuts at: 1684 1684
Size: 4664
MluI A'CGCG_T
Cuts at: 4097 4097
Size: 4664
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3335 3519 3335
Size: 184 4480
MscI TGG'CCA
Cuts at: 668 982 1543 2425 668
Size: 314 561 882 2907
Fragments arranged by size:
2907 882 561 314
MslI CAynn'nnrTG
Cuts at: 424 943 1066 2653 2780 424
Size: 519 123 1587 127 2308
Fragments arranged by size:
2308 1587 519 127 123
MspA1I CmG'CkG
Cuts at: 129 238 651 766 1167 1191 1204 1320 1573
Size: 109 413 115 401 24 13 116 253
Cuts at: 1573 1775 2089 2449 3462 3707 129
Size: 202 314 360 1013 245 1086
Fragments arranged by size:
1086 1013 413 401 360 314 253 245
202 116 115 109 24 13
NaeI GCC'GGC
Cuts at: 1610 2845 1610
Size: 1235 3429
NarI GG'CG_CC
Cuts at: 769 2095 2343 769
Size: 1326 248 3090
Fragments arranged by size:
3090 1326 248
NcoI C'CATG_G
Cuts at: 422 1121 422
Size: 699 3965
NgoMIV G'CCGG_C
Cuts at: 1608 2843 1608
Size: 1235 3429
NsiI A_TGCA'T
Cuts at: 113 113
Size: 4664
NspI r_CATG'y
Cuts at: 1717 2217 3124 1717
Size: 500 907 3257
Fragments arranged by size:
3257 907 500
PasI CC'CwG_GG
Cuts at: 168 168
Size: 4664
PciI A'CATG_T
Cuts at: 3120 3120
Size: 4664
PflMI CCAn_nnn'nTGG
Cuts at: 335 428 680 798 1624 335
Size: 93 252 118 826 3375
Fragments arranged by size:
3375 826 252 118 93
PfoI T'CCnGG_A
Cuts at: 755 755
Size: 4664
PmeI GTTT'AAAC
Cuts at: 1663 1663
Size: 4664
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1486 1518 3803 3835 1486
Size: 32 2285 32 2315
Fragments arranged by size:
2315 2285 32 32
PspOMI G'GGCC_C
Cuts at: 164 164
Size: 4664
PstI C_TGCA'G
Cuts at: 650 1711 2396 650
Size: 1061 685 2918
Fragments arranged by size:
2918 1061 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4664
PvuII CAG'CTG
Cuts at: 651 1167 1204 1573 2089 2449 651
Size: 516 37 369 516 360 2866
Fragments arranged by size:
2866 516 516 369 360 37
RsrII CG'GwC_CG
Cuts at: 2859 2859
Size: 4664
SacI G_AGCT'C
Cuts at: 1678 1678
Size: 4664
SacII CC_GC'GG
Cuts at: 239 239
Size: 4664
SalI G'TCGA_C
Cuts at: 1701 1701
Size: 4664
SapI GCTCTTCn'nnn_
Cuts at: 1561 2687 2897 1561
Size: 1126 210 3328
Fragments arranged by size:
3328 1126 210
SbfI CC_TGCA'GG
Cuts at: 1711 1711
Size: 4664
ScaI AGT'ACT
Cuts at: 4181 4181
Size: 4664
SfcI C'TryA_G
Cuts at: 33 646 1228 1707 2392 3385 3576 33
Size: 613 582 479 685 993 191 1121
Fragments arranged by size:
1121 993 685 613 582 479 191
SfoI GGC'GCC
Cuts at: 770 2096 2344 770
Size: 1326 248 3090
Fragments arranged by size:
3090 1326 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4664
SmaI CCC'GGG
Cuts at: 1686 1686
Size: 4664
SmlI C'TyrA_G
Cuts at: 1819 2734 3226 3488 1819
Size: 915 492 262 2995
Fragments arranged by size:
2995 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4664
SphI G_CATG'C
Cuts at: 1717 2217 1717
Size: 500 4164
StuI AGG'CCT
Cuts at: 395 703 1426 395
Size: 308 723 3633
Fragments arranged by size:
3633 723 308
StyI C'CwwG_G
Cuts at: 422 908 1121 1798 2152 422
Size: 486 213 677 354 2934
Fragments arranged by size:
2934 677 486 354 213
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 390 1231 2247 2380 2839 3048 390
Size: 841 1016 133 459 209 2006
Fragments arranged by size:
2006 1016 841 459 209 133
TatI w'GTAC_w
Cuts at: 4179 4179
Size: 4664
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1102 1134 4181 4213 1102
Size: 32 3047 32 1553
Fragments arranged by size:
3047 1553 32 32
Tth111I GACn'n_nGTC
Cuts at: 466 1528 2461 466
Size: 1062 933 2669
Fragments arranged by size:
2669 1062 933
XbaI T'CTAG_A
Cuts at: 1695 1695
Size: 4664
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 753 2159 753
Size: 1406 3258
XmaI C'CCGG_G
Cuts at: 1684 1684
Size: 4664
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AflII AlfI AscI AvrII BmgBI BmtI
BsiWI BsmBI BsrGI BssHII BstZ17I ClaI CspCI DraIII
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PmlI PpuMI PshAI PsiI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI