Restriction map for
pF1KE2393
(Circular) (Six-base) MAPSORT of: pF1KE2393.seq Check: 9440 from: 1 to: 4607
pF1KE2393 4607 bp
With 182 enzymes: SgfI *
January 26, 2012 13:39 ..
AccI GT'mk_AC
Cuts at: 494 1645 494
Size: 1151 3456
Acc65I G'GTAC_C
Cuts at: 1623 1623
Size: 4607
AclI AA'CG_TT
Cuts at: 4447 4447
Size: 4607
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2432 2864 3611 3879 2432
Size: 432 747 268 3160
Fragments arranged by size:
3160 747 432 268
AfeI AGC'GCT
Cuts at: 1111 1111
Size: 4607
AflIII A'CryG_T
Cuts at: 1959 3063 4040 1959
Size: 1104 977 2526
Fragments arranged by size:
2526 1104 977
AgeI A'CCGG_T
Cuts at: 2995 2995
Size: 4607
AleI CACnn'nnGTG
Cuts at: 1015 1015
Size: 4607
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 457 931 457
Size: 474 4133
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1474 1506 1474
Size: 32 4575
AlwNI CAG_nnn'CTG
Cuts at: 1049 1573 1912 3479 1049
Size: 524 339 1567 2177
Fragments arranged by size:
2177 1567 524 339
ApaI G_GGCC'C
Cuts at: 1583 1583
Size: 4607
ApaLI G'TGCA_C
Cuts at: 1063 3377 1063
Size: 2314 2293
ApoI r'AATT_y
Cuts at: 1611 3960 4216 1611
Size: 2349 256 2002
Fragments arranged by size:
2349 2002 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4607
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4607
AvaI C'yCGr_G
Cuts at: 1216 1627 1216
Size: 411 4196
BamHI G'GATC_C
Cuts at: 419 1632 419
Size: 1213 3394
BanI G'GyrC_C
Cuts at: 1095 1623 2037 2285 2320 1095
Size: 528 414 248 35 3382
Fragments arranged by size:
3382 528 414 248 35
BanII G_rGCy'C
Cuts at: 1538 1583 1621 2651 1538
Size: 45 38 1030 3494
Fragments arranged by size:
3494 1030 45 38
BbeI G_GCGC'C
Cuts at: 2041 2289 2041
Size: 248 4359
BbsI GAAGACnn'nnnn_
Cuts at: 858 1442 858
Size: 584 4023
BciVI GTATCCnnnnn_n'
Cuts at: 2505 3266 4607 2505
Size: 761 1341 2505
Fragments arranged by size:
2505 1341 761
BclI T'GATC_A
Cuts at: 2127 2127
Size: 4607
BglII A'GATC_T
Cuts at: 2122 4159 2122
Size: 2037 2570
BlpI GC'TnA_GC
Cuts at: 1719 1719
Size: 4607
Bme1580I G_kGCm'C
Cuts at: 1067 1528 1583 2232 2325 3381 1067
Size: 461 55 649 93 1056 2293
Fragments arranged by size:
2293 1056 649 461 93 55
BmgBI CAC'GTC
Cuts at: 771 771
Size: 4607
BmrI ACTGGGnnnn_n'
Cuts at: 692 1819 1900 2235 4379 692
Size: 1127 81 335 2144 920
Fragments arranged by size:
2144 1127 920 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2974 2974
Size: 4607
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1289 1289
Size: 4607
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 598 1783 2662 3154 3452 598
Size: 1185 879 492 298 1753
Fragments arranged by size:
1753 1185 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4311 4311
Size: 4607
BsaAI yAC'GTr
Cuts at: 765 1467 1960 765
Size: 702 493 3412
Fragments arranged by size:
3412 702 493
BsaBI GATnn'nnATC
Cuts at: 6 2121 4158 6
Size: 2115 2037 455
Fragments arranged by size:
2115 2037 455
BsaHI Gr'CG_yC
Cuts at: 667 2038 2286 2988 4484 667
Size: 1371 248 702 1496 790
Fragments arranged by size:
1496 1371 790 702 248
BsaWI w'CCGG_w
Cuts at: 512 1796 2317 2995 3269 3416 3827 512
Size: 1284 521 678 274 147 411 1292
Fragments arranged by size:
1292 1284 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 246 246
Size: 4607
BseYI C'CCAG_C
Cuts at: 243 296 757 1078 1192 1412 1592 2032 2790
Size: 53 461 321 114 220 180 440 758
Cuts at: 2790 3367 3863 243
Size: 577 496 987
Fragments arranged by size:
987 758 577 496 461 440 321 220
180 114 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 591 1105 1399 591
Size: 514 294 3799
Fragments arranged by size:
3799 514 294
BsiEI CG_ry'CG
Cuts at: 104 2149 2195 3403 104
Size: 2045 46 1208 1308
Fragments arranged by size:
2045 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 842 1058 1067 1621 2399 2589 3381 842
Size: 216 9 554 778 190 792 2068
Fragments arranged by size:
2068 792 778 554 216 190 9
Bsp1286I G_dGCh'C
Cuts at: 842 1058 1067 1528 1538 1583 1621 2232 2325
Size: 216 9 461 10 45 38 611 93
Cuts at: 2325 2399 2589 2651 3381 842
Size: 74 190 62 730 2068
Fragments arranged by size:
2068 730 611 461 216 190 93 74
62 45 38 10 9
BspEI T'CCGG_A
Cuts at: 3827 3827
Size: 4607
BspHI T'CATG_A
Cuts at: 329 662 1145 3783 329
Size: 333 483 2638 1153
Fragments arranged by size:
2638 1153 483 333
BspMI ACCTGCnnnn'nnnn_
Cuts at: 881 1657 1871 2173 2554 881
Size: 776 214 302 381 2934
Fragments arranged by size:
2934 776 381 302 214
BsrBI CCG'CTC
Cuts at: 50 623 1073 2899 2953 4437 4602 50
Size: 573 450 1826 54 1484 165 55
Fragments arranged by size:
1826 1484 573 450 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2519 2519
Size: 4607
BsrFI r'CCGG_y
Cuts at: 1309 1484 2605 2786 2995 1309
Size: 175 1121 181 209 2921
Fragments arranged by size:
2921 1121 209 181 175
BssHII G'CGCG_C
Cuts at: 114 116 114
Size: 2 4605
BssSI C'ACGA_G
Cuts at: 2878 3236 2878
Size: 358 4249
BstAPI GCAn_nnn'nTGC
Cuts at: 1128 1202 1984 1128
Size: 74 782 3751
Fragments arranged by size:
3751 782 74
BstBI TT'CG_AA
Cuts at: 2968 2968
Size: 4607
BstEII G'GTnAC_C
Cuts at: 1023 1023
Size: 4607
BstXI CCAn_nnnn'nTGG
Cuts at: 765 852 1020 765
Size: 87 168 4352
Fragments arranged by size:
4352 168 87
BstYI r'GATC_y
Cuts at: 1 419 1632 2099 2122 2457 2703 3715 3801
Size: 418 1213 467 23 335 246 1012 86
Cuts at: 3801 3813 4159 1
Size: 12 346 449
Fragments arranged by size:
1213 1012 467 449 418 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 415 415
Size: 4607
BtgI C'CryG_G
Cuts at: 1013 1091 1013
Size: 78 4529
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1392 2557 2738 2901 1392
Size: 1165 181 163 3098
Fragments arranged by size:
3098 1165 181 163
BtsI GCAGTG_nn'
Cuts at: 1850 1850
Size: 4607
DraI TTT'AAA
Cuts at: 1606 1606
Size: 4607
DrdI GACnn_nn'nnGTC
Cuts at: 1444 2313 3171 1444
Size: 869 858 2880
Fragments arranged by size:
2880 869 858
EaeI y'GGCC_r
Cuts at: 176 854 1175 2192 2366 2757 2784 4129 176
Size: 678 321 1017 174 391 27 1345 654
Fragments arranged by size:
1345 1017 678 654 391 321 174 27
EagI C'GGCC_G
Cuts at: 2192 2192
Size: 4607
EarI CTCTTCn'nnn_
Cuts at: 1147 1543 1558 2630 2840 1147
Size: 396 15 1072 210 2914
Fragments arranged by size:
2914 1072 396 210 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 229 2815 3125 3271 4417 229
Size: 2586 310 146 1146 419
Fragments arranged by size:
2586 1146 419 310 146
EcoICRI GAG'CTC
Cuts at: 1619 1619
Size: 4607
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1289 2432 2864 3611 3879 1289
Size: 1143 432 747 268 2017
Fragments arranged by size:
2017 1143 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1359 1359
Size: 4607
EcoO109I rG'GnC_Cy
Cuts at: 476 1579 1580 1746 476
Size: 1103 1 166 3337
Fragments arranged by size:
3337 1103 166 1
EcoRI G'AATT_C
Cuts at: 1611 1611
Size: 4607
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1131 2626 1131
Size: 1495 3112
FspI TGC'GCA
Cuts at: 2388 2388
Size: 4607
HaeII r_GCGC'y
Cuts at: 563 1113 1155 2041 2289 3311 4290 563
Size: 550 42 886 248 1022 979 880
Fragments arranged by size:
1022 979 886 880 550 248 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 311 343 2449 2479 2481 2511 311
Size: 32 2106 30 2 30 2407
Fragments arranged by size:
2407 2106 32 30 30 2
HincII GTy'rAC
Cuts at: 1646 1646
Size: 4607
KasI G'GCGC_C
Cuts at: 2037 2285 2037
Size: 248 4359
KpnI G_GTAC'C
Cuts at: 1627 1627
Size: 4607
MluI A'CGCG_T
Cuts at: 4040 4040
Size: 4607
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 542 905 1271 1392 3278 3462 542
Size: 363 366 121 1886 184 1687
Fragments arranged by size:
1886 1687 366 363 184 121
MscI TGG'CCA
Cuts at: 178 856 2368 178
Size: 678 1512 2417
Fragments arranged by size:
2417 1512 678
MslI CAynn'nnrTG
Cuts at: 913 1015 1510 2596 2723 913
Size: 102 495 1086 127 2797
Fragments arranged by size:
2797 1086 495 127 102
MspA1I CmG'CkG
Cuts at: 1718 2032 2392 3405 3650 1718
Size: 314 360 1013 245 2675
Fragments arranged by size:
2675 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1311 2788 1311
Size: 1477 3130
NarI GG'CG_CC
Cuts at: 2038 2286 2038
Size: 248 4359
NcoI C'CATG_G
Cuts at: 1013 1091 1013
Size: 78 4529
NgoMIV G'CCGG_C
Cuts at: 1309 2786 1309
Size: 1477 3130
NspI r_CATG'y
Cuts at: 1660 2160 3067 1660
Size: 500 907 3200
Fragments arranged by size:
3200 907 500
PasI CC'CwG_GG
Cuts at: 727 1100 1334 727
Size: 373 234 4000
Fragments arranged by size:
4000 373 234
PciI A'CATG_T
Cuts at: 3063 3063
Size: 4607
PflMI CCAn_nnn'nTGG
Cuts at: 344 1092 344
Size: 748 3859
PfoI T'CCnGG_A
Cuts at: 625 625
Size: 4607
PmeI GTTT'AAAC
Cuts at: 1606 1606
Size: 4607
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1270 1302 3746 3778 1270
Size: 32 2444 32 2099
Fragments arranged by size:
2444 2099 32 32
PpuMI rG'GwC_Cy
Cuts at: 476 476
Size: 4607
PspOMI G'GGCC_C
Cuts at: 1579 1579
Size: 4607
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 991 1023 1252 1284 991
Size: 32 229 32 4314
Fragments arranged by size:
4314 229 32 32
PstI C_TGCA'G
Cuts at: 284 1293 1654 2339 284
Size: 1009 361 685 2552
Fragments arranged by size:
2552 1009 685 361
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4607
PvuII CAG'CTG
Cuts at: 2032 2392 2032
Size: 360 4247
RsrII CG'GwC_CG
Cuts at: 2802 2802
Size: 4607
SacI G_AGCT'C
Cuts at: 1621 1621
Size: 4607
SalI G'TCGA_C
Cuts at: 1644 1644
Size: 4607
SapI GCTCTTCn'nnn_
Cuts at: 1543 2630 2840 1543
Size: 1087 210 3310
Fragments arranged by size:
3310 1087 210
SbfI CC_TGCA'GG
Cuts at: 1654 1654
Size: 4607
ScaI AGT'ACT
Cuts at: 583 4124 583
Size: 3541 1066
SfcI C'TryA_G
Cuts at: 33 280 1289 1650 2335 3328 3519 33
Size: 247 1009 361 685 993 191 1121
Fragments arranged by size:
1121 1009 993 685 361 247 191
SfoI GGC'GCC
Cuts at: 2039 2287 2039
Size: 248 4359
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4607
SmaI CCC'GGG
Cuts at: 1629 1629
Size: 4607
SmlI C'TyrA_G
Cuts at: 613 1762 2677 3169 3431 613
Size: 1149 915 492 262 1789
Fragments arranged by size:
1789 1149 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4607
SphI G_CATG'C
Cuts at: 1660 2160 1660
Size: 500 4107
StyI C'CwwG_G
Cuts at: 1013 1091 1741 2095 1013
Size: 78 650 354 3525
Fragments arranged by size:
3525 650 354 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 981 1099 1488 2190 2323 2782 2991 981
Size: 118 389 702 133 459 209 2597
Fragments arranged by size:
2597 702 459 389 209 133 118
TatI w'GTAC_w
Cuts at: 581 1268 4122 581
Size: 687 2854 1066
Fragments arranged by size:
2854 1066 687
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4124 4156 4124
Size: 32 4575
Tth111I GACn'n_nGTC
Cuts at: 655 2404 655
Size: 1749 2858
XbaI T'CTAG_A
Cuts at: 1638 1638
Size: 4607
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 678 2102 678
Size: 1424 3183
XmaI C'CCGG_G
Cuts at: 1627 1627
Size: 4607
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AscI AvrII BaeI BbvCI
BcgI BfrBI BglI BmtI Bpu10I BsaXI BsiWI BsmI
BsmBI BsrGI BstZ17I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PshAI PsiI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI