Restriction map for
pF1KE2400
(Circular) (Six-base) MAPSORT of: pF1KE2400.seq Check: 3015 from: 1 to: 5210
pF1KE2400 5210 bp
With 182 enzymes: SgfI *
July 3, 2020 20:21 ..
AatII G_ACGT'C
Cuts at: 1026 1026
Size: 5210
AccI GT'mk_AC
Cuts at: 2248 2248
Size: 5210
Acc65I G'GTAC_C
Cuts at: 2226 2226
Size: 5210
AclI AA'CG_TT
Cuts at: 5050 5050
Size: 5210
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 1107 1883 1963 3035 3467 4214 4482 172
Size: 935 776 80 1072 432 747 268 900
Fragments arranged by size:
1072 935 900 776 747 432 268 80
AfeI AGC'GCT
Cuts at: 439 439
Size: 5210
AflIII A'CryG_T
Cuts at: 1312 2562 3666 4643 1312
Size: 1250 1104 977 1879
Fragments arranged by size:
1879 1250 1104 977
AgeI A'CCGG_T
Cuts at: 3598 3598
Size: 5210
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2006 2006
Size: 5210
AlwNI CAG_nnn'CTG
Cuts at: 195 1165 2127 2515 4082 195
Size: 970 962 388 1567 1323
Fragments arranged by size:
1567 1323 970 962 388
ApaLI G'TGCA_C
Cuts at: 3980 3980
Size: 5210
ApoI r'AATT_y
Cuts at: 358 457 814 1215 1739 2214 4563 4819 358
Size: 99 357 401 524 475 2349 256 749
Fragments arranged by size:
2349 749 524 475 401 357 256 99
AseI AT'TA_AT
Cuts at: 20 1553 20
Size: 1533 3677
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5210
AvaI C'yCGr_G
Cuts at: 691 2230 691
Size: 1539 3671
BamHI G'GATC_C
Cuts at: 2235 2235
Size: 5210
BanI G'GyrC_C
Cuts at: 2070 2226 2640 2888 2923 2070
Size: 156 414 248 35 4357
Fragments arranged by size:
4357 414 248 156 35
BanII G_rGCy'C
Cuts at: 2224 3254 2224
Size: 1030 4180
BbeI G_GCGC'C
Cuts at: 2644 2892 2644
Size: 248 4962
BbsI GAAGACnn'nnnn_
Cuts at: 1450 1450
Size: 5210
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2052 2086 2052
Size: 34 5176
BciVI GTATCCnnnnn_n'
Cuts at: 1620 2025 3108 3869 5210 1620
Size: 405 1083 761 1341 1620
Fragments arranged by size:
1620 1341 1083 761 405
BclI T'GATC_A
Cuts at: 765 1928 1934 2730 765
Size: 1163 6 796 3245
Fragments arranged by size:
3245 1163 796 6
BglII A'GATC_T
Cuts at: 2725 4762 2725
Size: 2037 3173
BlpI GC'TnA_GC
Cuts at: 2322 2322
Size: 5210
Bme1580I G_kGCm'C
Cuts at: 1830 2075 2835 2928 3984 1830
Size: 245 760 93 1056 3056
Fragments arranged by size:
3056 1056 760 245 93
BmgBI CAC'GTC
Cuts at: 1315 1315
Size: 5210
BmrI ACTGGGnnnn_n'
Cuts at: 407 2422 2503 2838 4982 407
Size: 2015 81 335 2144 635
Fragments arranged by size:
2144 2015 635 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3577 3577
Size: 5210
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 217 1072 1084 187
Size: 30 855 12 4313
Fragments arranged by size:
4313 855 30 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1345 2386 3265 3757 4055 1345
Size: 1041 879 492 298 2500
Fragments arranged by size:
2500 1041 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 163 2032 4914 163
Size: 1869 2882 459
Fragments arranged by size:
2882 1869 459
BsaAI yAC'GTr
Cuts at: 2563 2563
Size: 5210
BsaBI GATnn'nnATC
Cuts at: 6 1933 2724 4761 6
Size: 1927 791 2037 455
Fragments arranged by size:
2037 1927 791 455
BsaHI Gr'CG_yC
Cuts at: 1023 2641 2889 3591 5087 1023
Size: 1618 248 702 1496 1146
Fragments arranged by size:
1618 1496 1146 702 248
BsaWI w'CCGG_w
Cuts at: 2399 2920 3598 3872 4019 4430 2399
Size: 521 678 274 147 411 3179
Fragments arranged by size:
3179 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1342 1493 1342
Size: 151 5059
BseYI C'CCAG_C
Cuts at: 434 2635 3393 3970 4466 434
Size: 2201 758 577 496 1178
Fragments arranged by size:
2201 1178 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2752 2798 4006 104
Size: 2648 46 1208 1308
Fragments arranged by size:
2648 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2224 3002 3192 3984 2224
Size: 778 190 792 3450
Fragments arranged by size:
3450 792 778 190
BsmI GAATG_Cn'
Cuts at: 1977 2099 1977
Size: 122 5088
BsmBI CGTCTCn'nnnn_
Cuts at: 147 147
Size: 5210
Bsp1286I G_dGCh'C
Cuts at: 1830 2075 2224 2835 2928 3002 3192 3254 3984
Size: 245 149 611 93 74 190 62 730
Cuts at: 3984 1830
Size: 3056
Fragments arranged by size:
3056 730 611 245 190 149 93 74
62
BspEI T'CCGG_A
Cuts at: 4430 4430
Size: 5210
BspHI T'CATG_A
Cuts at: 1109 1931 1940 4386 1109
Size: 822 9 2446 1933
Fragments arranged by size:
2446 1933 822 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 904 2260 2474 2776 3157 904
Size: 1356 214 302 381 2957
Fragments arranged by size:
2957 1356 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3502 3556 5040 5205 50
Size: 3452 54 1484 165 55
Fragments arranged by size:
3452 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 249 3122 249
Size: 2873 2337
BsrFI r'CCGG_y
Cuts at: 1259 3208 3389 3598 1259
Size: 1949 181 209 2871
Fragments arranged by size:
2871 1949 209 181
BsrGI T'GTAC_A
Cuts at: 366 366
Size: 5210
BssSI C'ACGA_G
Cuts at: 878 3481 3839 878
Size: 2603 358 2249
Fragments arranged by size:
2603 2249 358
BstAPI GCAn_nnn'nTGC
Cuts at: 896 2587 896
Size: 1691 3519
BstBI TT'CG_AA
Cuts at: 536 3571 536
Size: 3035 2175
BstYI r'GATC_y
Cuts at: 1 1247 1649 2235 2702 2725 3060 3306 4318
Size: 1246 402 586 467 23 335 246 1012
Cuts at: 4318 4404 4416 4762 1
Size: 86 12 346 449
Fragments arranged by size:
1246 1012 586 467 449 402 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 2173 2173
Size: 5210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3160 3341 3504 3160
Size: 181 163 4866
Fragments arranged by size:
4866 181 163
BtsI GCAGTG_nn'
Cuts at: 929 2453 929
Size: 1524 3686
DraI TTT'AAA
Cuts at: 675 2209 675
Size: 1534 3676
DraIII CAC_nnn'GTG
Cuts at: 824 2082 824
Size: 1258 3952
DrdI GACnn_nn'nnGTC
Cuts at: 2916 3774 2916
Size: 858 4352
EaeI y'GGCC_r
Cuts at: 1577 2795 2969 3360 3387 4732 1577
Size: 1218 174 391 27 1345 2055
Fragments arranged by size:
2055 1345 1218 391 174 27
EagI C'GGCC_G
Cuts at: 2795 2795
Size: 5210
EarI CTCTTCn'nnn_
Cuts at: 1182 1982 3233 3443 1182
Size: 800 1251 210 2949
Fragments arranged by size:
2949 1251 800 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3418 3728 3874 5020 3418
Size: 310 146 1146 3608
Fragments arranged by size:
3608 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2222 2222
Size: 5210
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 187 217 1072 1084 1107 1883 1963 3035
Size: 15 30 855 12 23 776 80 1072
Cuts at: 3035 3467 4214 4482 172
Size: 432 747 268 900
Fragments arranged by size:
1072 900 855 776 747 432 268 80
30 23 15 12
EcoO109I rG'GnC_Cy
Cuts at: 2349 2349
Size: 5210
EcoRI G'AATT_C
Cuts at: 2214 2214
Size: 5210
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1137 1888 3229 1137
Size: 751 1341 3118
Fragments arranged by size:
3118 1341 751
FspI TGC'GCA
Cuts at: 2991 2991
Size: 5210
HaeII r_GCGC'y
Cuts at: 441 2644 2892 3914 4893 441
Size: 2203 248 1022 979 758
Fragments arranged by size:
2203 1022 979 758 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 146 178 523 555 1175 1207 1636 1668 3052
Size: 32 345 32 620 32 429 32 1384
Cuts at: 3052 3082 3084 3114 146
Size: 30 2 30 2242
Fragments arranged by size:
2242 1384 620 429 345 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1194 2249 1194
Size: 1055 4155
KasI G'GCGC_C
Cuts at: 2640 2888 2640
Size: 248 4962
KpnI G_GTAC'C
Cuts at: 2230 2230
Size: 5210
MfeI C'AATT_G
Cuts at: 560 646 560
Size: 86 5124
MluI A'CGCG_T
Cuts at: 4643 4643
Size: 5210
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 666 3881 4065 666
Size: 3215 184 1811
Fragments arranged by size:
3215 1811 184
MscI TGG'CCA
Cuts at: 1579 2971 1579
Size: 1392 3818
MslI CAynn'nnrTG
Cuts at: 1301 1553 1955 3199 3326 1301
Size: 252 402 1244 127 3185
Fragments arranged by size:
3185 1244 402 252 127
MspA1I CmG'CkG
Cuts at: 2321 2635 2995 4008 4253 2321
Size: 314 360 1013 245 3278
Fragments arranged by size:
3278 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1261 3391 1261
Size: 2130 3080
NarI GG'CG_CC
Cuts at: 2641 2889 2641
Size: 248 4962
NgoMIV G'CCGG_C
Cuts at: 1259 3389 1259
Size: 2130 3080
NspI r_CATG'y
Cuts at: 2263 2763 3670 2263
Size: 500 907 3803
Fragments arranged by size:
3803 907 500
PciI A'CATG_T
Cuts at: 3666 3666
Size: 5210
PflMI CCAn_nnn'nTGG
Cuts at: 648 2082 648
Size: 1434 3776
PfoI T'CCnGG_A
Cuts at: 164 1373 164
Size: 1209 4001
PmeI GTTT'AAAC
Cuts at: 2209 2209
Size: 5210
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4349 4381 4349
Size: 32 5178
PstI C_TGCA'G
Cuts at: 1056 2257 2942 1056
Size: 1201 685 3324
Fragments arranged by size:
3324 1201 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5210
PvuII CAG'CTG
Cuts at: 2635 2995 2635
Size: 360 4850
RsrII CG'GwC_CG
Cuts at: 3405 3405
Size: 5210
SacI G_AGCT'C
Cuts at: 2224 2224
Size: 5210
SalI G'TCGA_C
Cuts at: 2247 2247
Size: 5210
SapI GCTCTTCn'nnn_
Cuts at: 1982 3233 3443 1982
Size: 1251 210 3749
Fragments arranged by size:
3749 1251 210
SbfI CC_TGCA'GG
Cuts at: 2257 2257
Size: 5210
ScaI AGT'ACT
Cuts at: 1721 4727 1721
Size: 3006 2204
SfcI C'TryA_G
Cuts at: 33 783 1052 2198 2253 2938 3931 4122 33
Size: 750 269 1146 55 685 993 191 1121
Fragments arranged by size:
1146 1121 993 750 685 269 191 55
SfoI GGC'GCC
Cuts at: 2642 2890 2642
Size: 248 4962
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5210
SmaI CCC'GGG
Cuts at: 693 2232 693
Size: 1539 3671
SmlI C'TyrA_G
Cuts at: 1324 2365 3280 3772 4034 1324
Size: 1041 915 492 262 2500
Fragments arranged by size:
2500 1041 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5210
SphI G_CATG'C
Cuts at: 2263 2763 2263
Size: 500 4710
SspI AAT'ATT
Cuts at: 1148 1148
Size: 5210
StuI AGG'CCT
Cuts at: 185 185
Size: 5210
StyI C'CwwG_G
Cuts at: 372 623 2344 2698 372
Size: 251 1721 354 2884
Fragments arranged by size:
2884 1721 354 251
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1210 2793 2926 3385 3594 1210
Size: 1583 133 459 209 2826
Fragments arranged by size:
2826 1583 459 209 133
TatI w'GTAC_w
Cuts at: 200 366 1582 1719 4725 200
Size: 166 1216 137 3006 685
Fragments arranged by size:
3006 1216 685 166 137
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1061 1093 4727 4759 1061
Size: 32 3634 32 1512
Fragments arranged by size:
3634 1512 32 32
Tth111I GACn'n_nGTC
Cuts at: 3007 3007
Size: 5210
XbaI T'CTAG_A
Cuts at: 2241 2241
Size: 5210
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2705 2705
Size: 5210
XmaI C'CCGG_G
Cuts at: 691 2230 691
Size: 1539 3671
XmnI GAAnn'nnTTC
Cuts at: 323 323
Size: 5210
ZraI GAC'GTC
Cuts at: 1024 1024
Size: 5210
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AloI ApaI AscI AvrII
BaeI BbvCI BfrBI BglI BmtI Bpu10I BsaXI BsgI
BsiWI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SwaI XhoI