Restriction map for
pF1KE2406
(Circular) (Six-base) MAPSORT of: pF1KE2406.seq Check: 7916 from: 1 to: 7604
pF1KE2406 7604 bp
With 182 enzymes: SgfI *
April 20, 2012 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 372 1173 372
Size: 801 6803
AccI GT'mk_AC
Cuts at: 4642 4642
Size: 7604
Acc65I G'GTAC_C
Cuts at: 2479 4620 2479
Size: 2141 5463
AclI AA'CG_TT
Cuts at: 7444 7444
Size: 7604
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 164 1357 1612 2938 3071 3380 5429 5861 6608
Size: 1193 255 1326 133 309 2049 432 747
Cuts at: 6608 6876 164
Size: 268 892
Fragments arranged by size:
2049 1326 1193 892 747 432 309 268
255 133
AflIII A'CryG_T
Cuts at: 3077 3518 4956 6060 7037 3077
Size: 441 1438 1104 977 3644
Fragments arranged by size:
3644 1438 1104 977 441
AgeI A'CCGG_T
Cuts at: 5992 5992
Size: 7604
AhdI GACnn_n'nnGTC
Cuts at: 3711 3711
Size: 7604
AleI CACnn'nnGTG
Cuts at: 1099 1099
Size: 7604
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1120 1152 1120
Size: 32 7572
AlwNI CAG_nnn'CTG
Cuts at: 2095 2149 2626 3244 4909 6476 2095
Size: 54 477 618 1665 1567 3223
Fragments arranged by size:
3223 1665 1567 618 477 54
ApaI G_GGCC'C
Cuts at: 1892 2140 3098 3107 3427 3566 3791 4306 4477
Size: 248 958 9 320 139 225 515 171
Cuts at: 4477 1892
Size: 5019
Fragments arranged by size:
5019 958 515 320 248 225 171 139
9
ApaLI G'TGCA_C
Cuts at: 6374 6374
Size: 7604
ApoI r'AATT_y
Cuts at: 481 530 639 3622 4608 6957 7213 481
Size: 49 109 2983 986 2349 256 872
Fragments arranged by size:
2983 2349 986 872 256 109 49
AseI AT'TA_AT
Cuts at: 20 20
Size: 7604
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7604
AvaI C'yCGr_G
Cuts at: 2258 2314 2741 3428 3558 3654 3782 3792 4514
Size: 56 427 687 130 96 128 10 722
Cuts at: 4514 4624 2258
Size: 110 5238
Fragments arranged by size:
5238 722 687 427 130 128 110 96
56 10
BamHI G'GATC_C
Cuts at: 3668 4629 3668
Size: 961 6643
BanI G'GyrC_C
Cuts at: 721 997 1472 1689 2479 2614 4081 4620 5034
Size: 276 475 217 790 135 1467 539 414
Cuts at: 5034 5282 5317 721
Size: 248 35 3008
Fragments arranged by size:
3008 1467 790 539 475 414 276 248
217 135 35
BanII G_rGCy'C
Cuts at: 357 445 631 706 835 1751 1892 2140 2168
Size: 88 186 75 129 916 141 248 28
Cuts at: 2168 2315 2520 3027 3098 3107 3124 3427 3566
Size: 147 205 507 71 9 17 303 139
Cuts at: 3566 3599 3791 3810 4306 4477 4618 5648 357
Size: 33 192 19 496 171 141 1030 2313
Fragments arranged by size:
2313 1030 916 507 496 303 248 205
192 186 171 147 141 141 139 129
88 75 71 33 28 19 17 9
BbeI G_GCGC'C
Cuts at: 5038 5286 5038
Size: 248 7356
BbsI GAAGACnn'nnnn_
Cuts at: 867 1268 1345 3368 4344 867
Size: 401 77 2023 976 4127
Fragments arranged by size:
4127 2023 976 401 77
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4110 4144 4110
Size: 34 7570
BciVI GTATCCnnnnn_n'
Cuts at: 1891 5502 6263 7604 1891
Size: 3611 761 1341 1891
Fragments arranged by size:
3611 1891 1341 761
BclI T'GATC_A
Cuts at: 3857 5124 3857
Size: 1267 6337
BglI GCCn_nnn'nGGC
Cuts at: 1481 1682 1967 2135 2270 2630 2693 2712 2777
Size: 201 285 168 135 360 63 19 65
Cuts at: 2777 2831 2840 2975 3102 3111 3786 3908 4310
Size: 54 9 135 127 9 675 122 402
Cuts at: 4310 4329 1481
Size: 19 4756
Fragments arranged by size:
4756 675 402 360 285 201 168 135
135 127 122 65 63 54 19 19
9 9
BglII A'GATC_T
Cuts at: 3190 4215 5119 7156 3190
Size: 1025 904 2037 3638
Fragments arranged by size:
3638 2037 1025 904
BlpI GC'TnA_GC
Cuts at: 4716 4716
Size: 7604
Bme1580I G_kGCm'C
Cuts at: 1477 1892 2140 2619 3098 3107 3427 3566 3791
Size: 415 248 479 479 9 320 139 225
Cuts at: 3791 4306 4450 4477 5229 5322 6378 1477
Size: 515 144 27 752 93 1056 2703
Fragments arranged by size:
2703 1056 752 515 479 479 415 320
248 225 144 139 93 27 9
BmgBI CAC'GTC
Cuts at: 3393 3393
Size: 7604
BmrI ACTGGGnnnn_n'
Cuts at: 702 2396 4816 4897 5232 7376 702
Size: 1694 2420 81 335 2144 930
Fragments arranged by size:
2420 2144 1694 930 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3618 3726 5971 3618
Size: 108 2245 5251
Fragments arranged by size:
5251 2245 108
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 429 1633 1663 1859 2048 2126 2444 2504
Size: 146 1204 30 196 189 78 318 60
Cuts at: 2504 2594 2606 3740 4232 4436 283
Size: 90 12 1134 492 204 3451
Fragments arranged by size:
3451 1204 1134 492 318 204 196 189
146 90 78 60 30 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 586 4780 5659 6151 6449 586
Size: 4194 879 492 298 1741
Fragments arranged by size:
4194 1741 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 676 1325 2161 2420 2449 2647 2855 7308 676
Size: 649 836 259 29 198 208 4453 972
Fragments arranged by size:
4453 972 836 649 259 208 198 29
BsaAI yAC'GTr
Cuts at: 2701 3078 4957 2701
Size: 377 1879 5348
Fragments arranged by size:
5348 1879 377
BsaBI GATnn'nnATC
Cuts at: 6 5118 7155 6
Size: 5112 2037 455
Fragments arranged by size:
5112 2037 455
BsaHI Gr'CG_yC
Cuts at: 898 5035 5283 5985 7481 898
Size: 4137 248 702 1496 1021
Fragments arranged by size:
4137 1496 1021 702 248
BsaWI w'CCGG_w
Cuts at: 462 3202 3845 4793 5314 5992 6266 6413 6824
Size: 2740 643 948 521 678 274 147 411
Cuts at: 6824 462
Size: 1242
Fragments arranged by size:
2740 1242 948 678 643 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 432 462 742 772 4566 4596 432
Size: 30 280 30 3794 30 3440
Fragments arranged by size:
3794 3440 280 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 832 930 1430 1591 1641 1759 3589 3607 3978
Size: 98 500 161 50 118 1830 18 371
Cuts at: 3978 3981 4064 832
Size: 3 83 4372
Fragments arranged by size:
4372 1830 500 371 161 118 98 83
50 18 3
BseYI C'CCAG_C
Cuts at: 973 1512 2634 4480 4561 5029 5787 6364 6860
Size: 539 1122 1846 81 468 758 577 496
Cuts at: 6860 973
Size: 1717
Fragments arranged by size:
1846 1717 1122 758 577 539 496 468
81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1074 1123 1711 4079 4094 1074
Size: 49 588 2368 15 4584
Fragments arranged by size:
4584 2368 588 49 15
BsiEI CG_ry'CG
Cuts at: 104 616 5146 5192 6400 104
Size: 512 4530 46 1208 1308
Fragments arranged by size:
4530 1308 1208 512 46
BsiHKAI G_wGCw'C
Cuts at: 445 706 835 1751 2520 3599 4045 4618 5396
Size: 261 129 916 769 1079 446 573 778
Cuts at: 5396 5586 6378 445
Size: 190 792 1671
Fragments arranged by size:
1671 1079 916 792 778 769 573 446
261 190 129
BsmI GAATG_Cn'
Cuts at: 3032 3506 3032
Size: 474 7130
Bsp1286I G_dGCh'C
Cuts at: 357 445 631 706 835 1477 1751 1892 2140
Size: 88 186 75 129 642 274 141 248
Cuts at: 2140 2168 2315 2520 2619 3027 3098 3107 3124
Size: 28 147 205 99 408 71 9 17
Cuts at: 3124 3427 3566 3599 3791 3810 4045 4306 4450
Size: 303 139 33 192 19 235 261 144
Cuts at: 4450 4477 4618 5229 5322 5396 5586 5648 6378
Size: 27 141 611 93 74 190 62 730
Cuts at: 6378 357
Size: 1583
Fragments arranged by size:
1583 730 642 611 408 303 274 261
248 235 205 192 190 186 147 144
141 141 139 129 99 93 88 75
74 71 62 33 28 27 19 17
9
BspEI T'CCGG_A
Cuts at: 462 3202 3845 6824 462
Size: 2740 643 2979 1242
Fragments arranged by size:
2979 2740 1242 643
BspHI T'CATG_A
Cuts at: 1193 6780 1193
Size: 5587 2017
BspMI ACCTGCnnnn'nnnn_
Cuts at: 372 1173 4654 4868 5170 5551 372
Size: 801 3481 214 302 381 2425
Fragments arranged by size:
3481 2425 801 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5896 5950 7434 7599 50
Size: 5846 54 1484 165 55
Fragments arranged by size:
5846 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 225 740 1562 2336 3684 4188 5516 225
Size: 515 822 774 1348 504 1328 2313
Fragments arranged by size:
2313 1348 1328 822 774 515 504
BsrFI r'CCGG_y
Cuts at: 257 2629 3730 4596 5602 5783 5992 257
Size: 2372 1101 866 1006 181 209 1869
Fragments arranged by size:
2372 1869 1101 1006 866 209 181
BssSI C'ACGA_G
Cuts at: 320 518 2000 5875 6233 320
Size: 198 1482 3875 358 1691
Fragments arranged by size:
3875 1691 1482 358 198
BstAPI GCAn_nnn'nTGC
Cuts at: 3820 4081 4981 3820
Size: 261 900 6443
Fragments arranged by size:
6443 900 261
BstBI TT'CG_AA
Cuts at: 5965 5965
Size: 7604
BstEII G'GTnAC_C
Cuts at: 2236 4546 2236
Size: 2310 5294
BstXI CCAn_nnnn'nTGG
Cuts at: 4405 4405
Size: 7604
BstYI r'GATC_y
Cuts at: 1 1944 2023 3190 3668 4215 4629 5096 5119
Size: 1943 79 1167 478 547 414 467 23
Cuts at: 5119 5454 5700 6712 6798 6810 7156 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1943 1167 1012 547 478 467 449 414
346 335 246 86 79 23 12
Bsu36I CC'TnA_GG
Cuts at: 2465 2925 2465
Size: 460 7144
BtgI C'CryG_G
Cuts at: 107 785 797 993 1694 1964 1991 2403 2591
Size: 678 12 196 701 270 27 412 188
Cuts at: 2591 3956 107
Size: 1365 3755
Fragments arranged by size:
3755 1365 701 678 412 270 196 188
27 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1124 1224 5554 5735 5898 1124
Size: 100 4330 181 163 2830
Fragments arranged by size:
4330 2830 181 163 100
BtsI GCAGTG_nn'
Cuts at: 1367 1401 1416 4847 1367
Size: 34 15 3431 4124
Fragments arranged by size:
4124 3431 34 15
ClaI AT'CG_AT
Cuts at: 2551 2551
Size: 7604
DraI TTT'AAA
Cuts at: 4603 4603
Size: 7604
DraIII CAC_nnn'GTG
Cuts at: 2083 3300 2083
Size: 1217 6387
DrdI GACnn_nn'nnGTC
Cuts at: 987 1922 3647 3767 5310 6168 987
Size: 935 1725 120 1543 858 2423
Fragments arranged by size:
2423 1725 1543 935 858 120
EaeI y'GGCC_r
Cuts at: 2487 3138 5189 5363 5754 5781 7126 2487
Size: 651 2051 174 391 27 1345 2965
Fragments arranged by size:
2965 2051 1345 651 391 174 27
EagI C'GGCC_G
Cuts at: 5189 5189
Size: 7604
EarI CTCTTCn'nnn_
Cuts at: 462 1558 3360 4038 5627 5837 462
Size: 1096 1802 678 1589 210 2229
Fragments arranged by size:
2229 1802 1589 1096 678 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1071 1588 3425 4154 4202 4581 5812 6122 6268
Size: 517 1837 729 48 379 1231 310 146
Cuts at: 6268 7414 1071
Size: 1146 1261
Fragments arranged by size:
1837 1261 1231 1146 729 517 379 310
146 48
EcoICRI GAG'CTC
Cuts at: 443 704 833 1749 2518 3597 4616 443
Size: 261 129 916 769 1079 1019 3431
Fragments arranged by size:
3431 1079 1019 916 769 261 129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 164 283 429 1357 1612 1633 1663 1859 2048
Size: 119 146 928 255 21 30 196 189
Cuts at: 2048 2126 2444 2504 2594 2606 2938 3071 3380
Size: 78 318 60 90 12 332 133 309
Cuts at: 3380 3740 4232 4436 5429 5861 6608 6876 164
Size: 360 492 204 993 432 747 268 892
Fragments arranged by size:
993 928 892 747 492 432 360 332
318 309 268 255 204 196 189 146
133 119 90 78 60 30 21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 402 3050 402
Size: 2648 4956
EcoO109I rG'GnC_Cy
Cuts at: 791 803 1843 1889 1960 1987 1996 2014 2065
Size: 12 1040 46 71 27 9 18 51
Cuts at: 2065 2101 2136 2137 2146 2227 2254 2335 2416
Size: 36 35 1 9 81 27 81 81
Cuts at: 2416 2452 2461 2569 2578 2596 2605 2623 2677
Size: 36 9 108 9 18 9 18 54
Cuts at: 2677 2713 2737 2851 2869 2878 3094 3095 3104
Size: 36 24 114 18 9 216 1 9
Cuts at: 3104 3214 3241 3424 3467 3562 3563 3649 3705
Size: 110 27 183 43 95 1 86 56
Cuts at: 3705 3751 3760 3778 3787 3788 3910 3928 4111
Size: 46 9 18 9 1 122 18 183
Cuts at: 4111 4269 4302 4420 4456 4473 4474 4743 791
Size: 158 33 118 36 17 1 269 3652
Fragments arranged by size:
3652 1040 269 216 183 183 158 122
118 114 110 108 95 86 81 81
81 71 56 54 51 46 46 43
36 36 36 36 35 33 27 27
27 24 18 18 18 18 18 18
17 12 9 9 9 9 9 9
9 9 9 1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 639 3622 4608 639
Size: 2983 986 3635
Fragments arranged by size:
3635 2983 986
EcoRV GAT'ATC
Cuts at: 1377 1377
Size: 7604
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2200 2680 5623 2200
Size: 480 2943 4181
Fragments arranged by size:
4181 2943 480
FspI TGC'GCA
Cuts at: 3886 5385 3886
Size: 1499 6105
HaeII r_GCGC'y
Cuts at: 1439 1465 5038 5286 6308 7287 1439
Size: 26 3573 248 1022 979 1756
Fragments arranged by size:
3573 1756 1022 979 248 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2533 2565 3411 3443 3697 3729 4434 4466 5446
Size: 32 846 32 254 32 705 32 980
Cuts at: 5446 5476 5478 5508 2533
Size: 30 2 30 4629
Fragments arranged by size:
4629 980 846 705 254 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 4643 4643
Size: 7604
KasI G'GCGC_C
Cuts at: 5034 5282 5034
Size: 248 7356
KpnI G_GTAC'C
Cuts at: 2483 4624 2483
Size: 2141 5463
MluI A'CGCG_T
Cuts at: 7037 7037
Size: 7604
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 671 2825 3618 6275 6459 671
Size: 2154 793 2657 184 1816
Fragments arranged by size:
2657 2154 1816 793 184
MscI TGG'CCA
Cuts at: 3140 5365 3140
Size: 2225 5379
MslI CAynn'nnrTG
Cuts at: 431 1099 3076 3697 5593 5720 431
Size: 668 1977 621 1896 127 2315
Fragments arranged by size:
2315 1977 1896 668 621 127
MspA1I CmG'CkG
Cuts at: 293 787 1208 1496 1696 2117 2638 3384 3826
Size: 494 421 288 200 421 521 746 442
Cuts at: 3826 4715 5029 5389 6402 6647 293
Size: 889 314 360 1013 245 1250
Fragments arranged by size:
1250 1013 889 746 521 494 442 421
421 360 314 288 245 200
NaeI GCC'GGC
Cuts at: 259 2631 3732 5785 259
Size: 2372 1101 2053 2078
Fragments arranged by size:
2372 2078 2053 1101
NarI GG'CG_CC
Cuts at: 5035 5283 5035
Size: 248 7356
NcoI C'CATG_G
Cuts at: 107 797 993 1964 1991 2591 107
Size: 690 196 971 27 600 5120
Fragments arranged by size:
5120 971 690 600 196 27
NdeI CA'TA_TG
Cuts at: 1020 1020
Size: 7604
NgoMIV G'CCGG_C
Cuts at: 257 2629 3730 5783 257
Size: 2372 1101 2053 2078
Fragments arranged by size:
2372 2078 2053 1101
NspI r_CATG'y
Cuts at: 957 1041 3174 3522 4173 4260 4657 5157 6064
Size: 84 2133 348 651 87 397 500 907
Cuts at: 6064 957
Size: 2497
Fragments arranged by size:
2497 2133 907 651 500 397 348 87
84
PasI CC'CwG_GG
Cuts at: 1867 1885 2457 2982 3100 3109 3775 3934 4068
Size: 18 572 525 118 9 666 159 134
Cuts at: 4068 4426 1867
Size: 358 5045
Fragments arranged by size:
5045 666 572 525 358 159 134 118
18 9
PciI A'CATG_T
Cuts at: 3518 6060 3518
Size: 2542 5062
PflMI CCAn_nnn'nTGG
Cuts at: 569 1686 569
Size: 1117 6487
PfoI T'CCnGG_A
Cuts at: 740 2019 2583 3756 4452 740
Size: 1279 564 1173 696 3892
Fragments arranged by size:
3892 1279 1173 696 564
PmeI GTTT'AAAC
Cuts at: 4603 4603
Size: 7604
PmlI CAC'GTG
Cuts at: 2701 2701
Size: 7604
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6743 6775 6743
Size: 32 7572
PpuMI rG'GwC_Cy
Cuts at: 791 803 1996 2065 2101 2146 2227 2254 2335
Size: 12 1193 69 36 45 81 27 81
Cuts at: 2335 2416 2452 2461 2578 2596 2605 2677 2737
Size: 81 36 9 117 18 9 72 60
Cuts at: 2737 2851 2869 3214 3241 3467 3649 3705 3760
Size: 114 18 345 27 226 182 56 55
Cuts at: 3760 3928 4111 4420 4456 791
Size: 168 183 309 36 3939
Fragments arranged by size:
3939 1193 345 309 226 183 182 168
117 114 81 81 81 72 69 60
56 55 45 36 36 36 27 27
18 18 12 9 9
PshAI GACnn'nnGTC
Cuts at: 2867 3395 4109 2867
Size: 528 714 6362
Fragments arranged by size:
6362 714 528
PspOMI G'GGCC_C
Cuts at: 1888 2136 3094 3103 3423 3562 3787 4302 4473
Size: 248 958 9 320 139 225 515 171
Cuts at: 4473 1888
Size: 5019
Fragments arranged by size:
5019 958 515 320 248 225 171 139
9
PspXI vC'TCGA_Gb
Cuts at: 2258 3782 4514 2258
Size: 1524 732 5348
Fragments arranged by size:
5348 1524 732
PstI C_TGCA'G
Cuts at: 548 1374 1495 3383 4101 4651 5336 548
Size: 826 121 1888 718 550 685 2816
Fragments arranged by size:
2816 1888 826 718 685 550 121
PvuI CG_AT'CG
Cuts at: 104 616 104
Size: 512 7092
PvuII CAG'CTG
Cuts at: 1496 2638 3826 5029 5389 1496
Size: 1142 1188 1203 360 3711
Fragments arranged by size:
3711 1203 1188 1142 360
RsrII CG'GwC_CG
Cuts at: 5799 5799
Size: 7604
SacI G_AGCT'C
Cuts at: 445 706 835 1751 2520 3599 4618 445
Size: 261 129 916 769 1079 1019 3431
Fragments arranged by size:
3431 1079 1019 916 769 261 129
SacII CC_GC'GG
Cuts at: 788 1697 788
Size: 909 6695
SalI G'TCGA_C
Cuts at: 4641 4641
Size: 7604
SanDI GG'GwC_CC
Cuts at: 2101 2596 3214 3467 3928 4111 2101
Size: 495 618 253 461 183 5594
Fragments arranged by size:
5594 618 495 461 253 183
SapI GCTCTTCn'nnn_
Cuts at: 1558 4038 5627 5837 1558
Size: 2480 1589 210 3325
Fragments arranged by size:
3325 2480 1589 210
SbfI CC_TGCA'GG
Cuts at: 4651 4651
Size: 7604
ScaI AGT'ACT
Cuts at: 3415 7121 3415
Size: 3706 3898
SexAI A'CCwGG_T
Cuts at: 2061 2079 3228 4488 2061
Size: 18 1149 1260 5177
Fragments arranged by size:
5177 1260 1149 18
SfcI C'TryA_G
Cuts at: 33 544 1370 1386 1491 2150 3379 4026 4097
Size: 511 826 16 105 659 1229 647 71
Cuts at: 4097 4362 4647 5332 6325 6516 33
Size: 265 285 685 993 191 1121
Fragments arranged by size:
1229 1121 993 826 685 659 647 511
285 265 191 105 71 16
SfiI GGCCn_nnn'nGGCC
Cuts at: 1967 2135 2630 2693 2712 2777 2831 2840 2975
Size: 168 495 63 19 65 54 9 135
Cuts at: 2975 3102 3111 3786 3908 4310 4329 1967
Size: 127 9 675 122 402 19 5242
Fragments arranged by size:
5242 675 495 402 168 135 127 122
65 63 54 19 19 9 9
SfoI GGC'GCC
Cuts at: 5036 5284 5036
Size: 248 7356
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7604
SmaI CCC'GGG
Cuts at: 2316 3560 3656 3794 4626 2316
Size: 1244 96 138 832 5294
Fragments arranged by size:
5294 1244 832 138 96
SmlI C'TyrA_G
Cuts at: 601 2258 3782 4514 4759 5674 6166 6428 601
Size: 1657 1524 732 245 915 492 262 1777
Fragments arranged by size:
1777 1657 1524 915 732 492 262 245
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7604
SphI G_CATG'C
Cuts at: 957 4260 4657 5157 957
Size: 3303 397 500 3404
Fragments arranged by size:
3404 3303 500 397
StuI AGG'CCT
Cuts at: 1310 2264 2906 1310
Size: 954 642 6008
Fragments arranged by size:
6008 954 642
StyI C'CwwG_G
Cuts at: 107 797 993 1199 1450 1892 1964 1991 2160
Size: 690 196 206 251 442 72 27 169
Cuts at: 2160 2358 2591 2855 2865 2892 4738 5092 107
Size: 198 233 264 10 27 1846 354 2619
Fragments arranged by size:
2619 1846 690 442 354 264 251 233
206 198 196 169 72 27 27 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 924 1916 4060 5187 5320 5779 5988 924
Size: 992 2144 1127 133 459 209 2540
Fragments arranged by size:
2540 2144 1127 992 459 209 133
TatI w'GTAC_w
Cuts at: 3155 3167 3413 7119 3155
Size: 12 246 3706 3640
Fragments arranged by size:
3706 3640 246 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 432 464 644 676 2069 2101 3653 3685 7121
Size: 32 180 32 1393 32 1552 32 3436
Cuts at: 7121 7153 432
Size: 32 883
Fragments arranged by size:
3436 1552 1393 883 180 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 509 1344 4552 5401 509
Size: 835 3208 849 2712
Fragments arranged by size:
3208 2712 849 835
XbaI T'CTAG_A
Cuts at: 4635 4635
Size: 7604
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 566 1176 1466 3680 3696 4558 5099 566
Size: 610 290 2214 16 862 541 3071
Fragments arranged by size:
3071 2214 862 610 541 290 16
XhoI C'TCGA_G
Cuts at: 2258 3782 4514 2258
Size: 1524 732 5348
Fragments arranged by size:
5348 1524 732
XmaI C'CCGG_G
Cuts at: 2314 3558 3654 3792 4624 2314
Size: 1244 96 138 832 5294
Fragments arranged by size:
5294 1244 832 138 96
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AlfI AscI AvrII BaeI BbvCI
BfrBI BmtI Bpu10I BsiWI BsmBI BsrGI BssHII BstZ17I
CspCI FseI FspAI HindIII HpaI MfeI NheI NotI
NruI NsiI PacI PsiI PsrI SgrAI SnaBI SrfI
SspI SwaI XmnI ZraI