Restriction map for
pF1KE2408
(Circular) (Six-base) MAPSORT of: pF1KE2408.seq Check: 1340 from: 1 to: 9617
pF1KE2408 9617 bp
With 182 enzymes: SgfI *
June 11, 2012 13:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2259 2259
Size: 9617
AatII G_ACGT'C
Cuts at: 2700 2700
Size: 9617
AccI GT'mk_AC
Cuts at: 342 1237 2709 6655 342
Size: 895 1472 3946 3304
Fragments arranged by size:
3946 3304 1472 895
Acc65I G'GTAC_C
Cuts at: 6633 6633
Size: 9617
AclI AA'CG_TT
Cuts at: 9457 9457
Size: 9617
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 170 1379 1480 1940 2117 2480 2505 3718 3725
Size: 1209 101 460 177 363 25 1213 7
Cuts at: 3725 3896 4471 4951 4984 5159 6170 6251 7442
Size: 171 575 480 33 175 1011 81 1191
Cuts at: 7442 7874 8621 8889 170
Size: 432 747 268 898
Fragments arranged by size:
1213 1209 1191 1011 898 747 575 480
460 432 363 268 177 175 171 101
81 33 25 7
AflIII A'CryG_T
Cuts at: 3302 6969 8073 9050 3302
Size: 3667 1104 977 3869
Fragments arranged by size:
3869 3667 1104 977
AgeI A'CCGG_T
Cuts at: 8005 8005
Size: 9617
AleI CACnn'nnGTG
Cuts at: 1223 5023 1223
Size: 3800 5817
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4608 4608
Size: 9617
AlwNI CAG_nnn'CTG
Cuts at: 2215 3544 5581 6922 8489 2215
Size: 1329 2037 1341 1567 3343
Fragments arranged by size:
3343 2037 1567 1341 1329
ApaLI G'TGCA_C
Cuts at: 4593 5681 8387 4593
Size: 1088 2706 5823
Fragments arranged by size:
5823 2706 1088
ApoI r'AATT_y
Cuts at: 370 512 2734 2996 5348 5656 6621 8970 9226
Size: 142 2222 262 2352 308 965 2349 256
Cuts at: 9226 370
Size: 761
Fragments arranged by size:
2352 2349 2222 965 761 308 262 256
142
AseI AT'TA_AT
Cuts at: 20 1664 20
Size: 1644 7973
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9617
AvaI C'yCGr_G
Cuts at: 2846 5444 6637 2846
Size: 2598 1193 5826
Fragments arranged by size:
5826 2598 1193
BamHI G'GATC_C
Cuts at: 6642 6642
Size: 9617
BanI G'GyrC_C
Cuts at: 2399 6633 7047 7295 7330 2399
Size: 4234 414 248 35 4686
Fragments arranged by size:
4686 4234 414 248 35
BanII G_rGCy'C
Cuts at: 2991 5795 6631 7661 2991
Size: 2804 836 1030 4947
Fragments arranged by size:
4947 2804 1030 836
BbeI G_GCGC'C
Cuts at: 2403 7051 7299 2403
Size: 4648 248 4721
Fragments arranged by size:
4721 4648 248
BbsI GAAGACnn'nnnn_
Cuts at: 2652 3093 3305 3706 4079 5675 6164 2652
Size: 441 212 401 373 1596 489 6105
Fragments arranged by size:
6105 1596 489 441 401 373 212
BbvCI CC'TCA_GC
Cuts at: 135 2891 135
Size: 2756 6861
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 5662 5696 5662
Size: 34 9583
BciVI GTATCCnnnnn_n'
Cuts at: 3565 6473 7515 8276 9617 3565
Size: 2908 1042 761 1341 3565
Fragments arranged by size:
3565 2908 1341 1042 761
BclI T'GATC_A
Cuts at: 3758 7137 3758
Size: 3379 6238
BfrBI ATG'CAT
Cuts at: 6352 6352
Size: 9617
BglII A'GATC_T
Cuts at: 4452 5520 7132 9169 4452
Size: 1068 1612 2037 4900
Fragments arranged by size:
4900 2037 1612 1068
BlpI GC'TnA_GC
Cuts at: 2990 3309 6729 2990
Size: 319 3420 5878
Fragments arranged by size:
5878 3420 319
Bme1580I G_kGCm'C
Cuts at: 2174 3955 4597 5685 7242 7335 8391 2174
Size: 1781 642 1088 1557 93 1056 3400
Fragments arranged by size:
3400 1781 1557 1088 1056 642 93
BmrI ACTGGGnnnn_n'
Cuts at: 325 517 1822 1898 2238 4639 6829 6910 7245
Size: 192 1305 76 340 2401 2190 81 335
Cuts at: 7245 9389 325
Size: 2144 553
Fragments arranged by size:
2401 2190 2144 1305 553 340 335 192
81 76
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7984 7984
Size: 9617
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 236 3710 3947 5132 5771 137
Size: 99 3474 237 1185 639 3983
Fragments arranged by size:
3983 3474 1185 639 237 99
Bpu10I CC'TnA_GC
Cuts at: 135 357 408 720 2891 5794 6047 135
Size: 222 51 312 2171 2903 253 3705
Fragments arranged by size:
3705 2903 2171 312 253 222 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 858 2487 2765 3849 5360 5371 6190 6793 7672
Size: 1629 278 1084 1511 11 819 603 879
Cuts at: 7672 8164 8462 858
Size: 492 298 2013
Fragments arranged by size:
2013 1629 1511 1084 879 819 603 492
298 278 11
BsaI GGTCTCn'nnnn_
Cuts at: 9321 9321
Size: 9617
BsaAI yAC'GTr
Cuts at: 6970 6970
Size: 9617
BsaBI GATnn'nnATC
Cuts at: 6 7131 9168 6
Size: 7125 2037 455
Fragments arranged by size:
7125 2037 455
BsaHI Gr'CG_yC
Cuts at: 2400 2697 4257 7048 7296 7998 9494 2400
Size: 297 1560 2791 248 702 1496 2523
Fragments arranged by size:
2791 2523 1560 1496 702 297 248
BsaWI w'CCGG_w
Cuts at: 6806 7327 8005 8279 8426 8837 6806
Size: 521 678 274 147 411 7586
Fragments arranged by size:
7586 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3945 3975 4890 4920 5449 5479 3945
Size: 30 915 30 529 30 8083
Fragments arranged by size:
8083 915 529 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 135 1613 2908 3523 3846 4004 4853 4898 135
Size: 1478 1295 615 323 158 849 45 4854
Fragments arranged by size:
4854 1478 1295 849 615 323 158 45
BseYI C'CCAG_C
Cuts at: 271 534 1133 4902 7042 7800 8377 8873 271
Size: 263 599 3769 2140 758 577 496 1015
Fragments arranged by size:
3769 2140 1015 758 599 577 496 263
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 464 3418 5819 464
Size: 2954 2401 4262
Fragments arranged by size:
4262 2954 2401
BsiEI CG_ry'CG
Cuts at: 104 7159 7205 8413 104
Size: 7055 46 1208 1308
Fragments arranged by size:
7055 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2991 4597 5685 6631 7409 7599 8391 2991
Size: 1606 1088 946 778 190 792 4217
Fragments arranged by size:
4217 1606 1088 946 792 778 190
BsmI GAATG_Cn'
Cuts at: 363 1417 2582 5852 363
Size: 1054 1165 3270 4128
Fragments arranged by size:
4128 3270 1165 1054
BsmBI CGTCTCn'nnnn_
Cuts at: 2688 4217 2688
Size: 1529 8088
Bsp1286I G_dGCh'C
Cuts at: 2174 2991 3955 4597 5685 5795 6631 7242 7335
Size: 817 964 642 1088 110 836 611 93
Cuts at: 7335 7409 7599 7661 8391 2174
Size: 74 190 62 730 3400
Fragments arranged by size:
3400 1088 964 836 817 730 642 611
190 110 93 74 62
BspEI T'CCGG_A
Cuts at: 8837 8837
Size: 9617
BspHI T'CATG_A
Cuts at: 546 6342 8793 546
Size: 5796 2451 1370
Fragments arranged by size:
5796 2451 1370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2259 5193 6667 6881 7183 7564 2259
Size: 2934 1474 214 302 381 4312
Fragments arranged by size:
4312 2934 1474 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3192 5751 7909 7963 9447 9612 50
Size: 3142 2559 2158 54 1484 165 55
Fragments arranged by size:
3142 2559 2158 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 827 1673 2633 2733 4219 4668 5489 6041 7529
Size: 846 960 100 1486 449 821 552 1488
Cuts at: 7529 827
Size: 2915
Fragments arranged by size:
2915 1488 1486 960 846 821 552 449
100
BsrFI r'CCGG_y
Cuts at: 4259 7615 7796 8005 4259
Size: 3356 181 209 5871
Fragments arranged by size:
5871 3356 209 181
BssSI C'ACGA_G
Cuts at: 7888 8246 7888
Size: 358 9259
BstAPI GCAn_nnn'nTGC
Cuts at: 2425 2577 6994 2425
Size: 152 4417 5048
Fragments arranged by size:
5048 4417 152
BstBI TT'CG_AA
Cuts at: 7978 7978
Size: 9617
BstEII G'GTnAC_C
Cuts at: 125 4120 125
Size: 3995 5622
BstXI CCAn_nnnn'nTGG
Cuts at: 1591 3048 5025 1591
Size: 1457 1977 6183
Fragments arranged by size:
6183 1977 1457
BstYI r'GATC_y
Cuts at: 1 1390 4452 4915 5520 6642 7109 7132 7467
Size: 1389 3062 463 605 1122 467 23 335
Cuts at: 7467 7713 8725 8811 8823 9169 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
3062 1389 1122 1012 605 467 463 449
346 335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1148 1148
Size: 9617
BtgI C'CryG_G
Cuts at: 107 695 107
Size: 588 9029
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 7567 7748 7911 7567
Size: 181 163 9273
Fragments arranged by size:
9273 181 163
BtsI GCAGTG_nn'
Cuts at: 900 2587 5685 5835 6860 900
Size: 1687 3098 150 1025 3657
Fragments arranged by size:
3657 3098 1687 1025 150
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 201 231 236 266 201
Size: 30 5 30 9552
Fragments arranged by size:
9552 30 30 5
DraI TTT'AAA
Cuts at: 4415 6616 4415
Size: 2201 7416
DraIII CAC_nnn'GTG
Cuts at: 4810 4810
Size: 9617
DrdI GACnn_nn'nnGTC
Cuts at: 4866 6108 7323 8181 4866
Size: 1242 1215 858 6302
Fragments arranged by size:
6302 1242 1215 858
EaeI y'GGCC_r
Cuts at: 4239 6555 7202 7376 7767 7794 9139 4239
Size: 2316 647 174 391 27 1345 4717
Fragments arranged by size:
4717 2316 1345 647 391 174 27
EagI C'GGCC_G
Cuts at: 7202 7202
Size: 9617
EarI CTCTTCn'nnn_
Cuts at: 1447 1934 2313 2594 3929 4136 4970 5695 5768
Size: 487 379 281 1335 207 834 725 73
Cuts at: 5768 7640 7850 1447
Size: 1872 210 3214
Fragments arranged by size:
3214 1872 1335 834 725 487 379 281
210 207 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 7825 8135 8281 9427 7825
Size: 310 146 1146 8015
Fragments arranged by size:
8015 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2989 6629 2989
Size: 3640 5977
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 170 236 1379 1480 1940 2117 2480 2505
Size: 33 66 1143 101 460 177 363 25
Cuts at: 2505 3710 3718 3725 3896 3947 4471 4951 4984
Size: 1205 8 7 171 51 524 480 33
Cuts at: 4984 5132 5159 5771 6170 6251 7442 7874 8621
Size: 148 27 612 399 81 1191 432 747
Cuts at: 8621 8889 137
Size: 268 865
Fragments arranged by size:
1205 1191 1143 865 747 612 524 480
460 432 399 363 268 177 171 148
101 81 66 51 33 33 27 25
8 7
EcoNI CCTnn'n_nnAGG
Cuts at: 1462 5066 5509 1462
Size: 3604 443 5570
Fragments arranged by size:
5570 3604 443
EcoO109I rG'GnC_Cy
Cuts at: 5283 6300 6756 5283
Size: 1017 456 8144
Fragments arranged by size:
8144 1017 456
EcoRI G'AATT_C
Cuts at: 512 2734 6621 512
Size: 2222 3887 3508
Fragments arranged by size:
3887 3508 2222
EcoRV GAT'ATC
Cuts at: 2499 4098 2499
Size: 1599 8018
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 223 3118 3538 3926 5959 6281 7636 223
Size: 2895 420 388 2033 322 1355 2204
Fragments arranged by size:
2895 2204 2033 1355 420 388 322
FspI TGC'GCA
Cuts at: 7398 7398
Size: 9617
HaeII r_GCGC'y
Cuts at: 2403 7051 7299 8321 9300 2403
Size: 4648 248 1022 979 2720
Fragments arranged by size:
4648 2720 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1972 1987 2004 2019 2682 2714 3344 3376 3805
Size: 15 17 15 663 32 630 32 429
Cuts at: 3805 3837 5449 5481 5569 5601 6145 6151 6177
Size: 32 1612 32 88 32 544 6 26
Cuts at: 6177 6183 7459 7489 7491 7521 1972
Size: 6 1276 30 2 30 4068
Fragments arranged by size:
4068 1612 1276 663 630 544 429 88
32 32 32 32 32 30 30 26
17 15 15 6 6 2
HincII GTy'rAC
Cuts at: 786 2710 4646 4844 6656 786
Size: 1924 1936 198 1812 3747
Fragments arranged by size:
3747 1936 1924 1812 198
HindIII A'AGCT_T
Cuts at: 2812 3707 3737 4519 5291 2812
Size: 895 30 782 772 7138
Fragments arranged by size:
7138 895 782 772 30
KasI G'GCGC_C
Cuts at: 2399 7047 7295 2399
Size: 4648 248 4721
Fragments arranged by size:
4721 4648 248
KpnI G_GTAC'C
Cuts at: 6637 6637
Size: 9617
MluI A'CGCG_T
Cuts at: 9050 9050
Size: 9617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2680 5545 8288 8472 2680
Size: 2865 2743 184 3825
Fragments arranged by size:
3825 2865 2743 184
MscI TGG'CCA
Cuts at: 4241 6557 7378 4241
Size: 2316 821 6480
Fragments arranged by size:
6480 2316 821
MslI CAynn'nnrTG
Cuts at: 1223 3394 5023 6347 7606 7733 1223
Size: 2171 1629 1324 1259 127 3107
Fragments arranged by size:
3107 2171 1629 1324 1259 127
MspA1I CmG'CkG
Cuts at: 271 3541 5191 6728 7042 7402 8415 8660 271
Size: 3270 1650 1537 314 360 1013 245 1228
Fragments arranged by size:
3270 1650 1537 1228 1013 360 314 245
NaeI GCC'GGC
Cuts at: 7798 7798
Size: 9617
NarI GG'CG_CC
Cuts at: 2400 7048 7296 2400
Size: 4648 248 4721
Fragments arranged by size:
4721 4648 248
NcoI C'CATG_G
Cuts at: 107 695 107
Size: 588 9029
NgoMIV G'CCGG_C
Cuts at: 7796 7796
Size: 9617
NsiI A_TGCA'T
Cuts at: 6354 6354
Size: 9617
NspI r_CATG'y
Cuts at: 3306 3776 5689 6670 7170 8077 3306
Size: 470 1913 981 500 907 4846
Fragments arranged by size:
4846 1913 981 907 500 470
PciI A'CATG_T
Cuts at: 3302 8073 3302
Size: 4771 4846
PflMI CCAn_nnn'nTGG
Cuts at: 337 771 2299 2722 4579 337
Size: 434 1528 423 1857 5375
Fragments arranged by size:
5375 1857 1528 434 423
PfoI T'CCnGG_A
Cuts at: 2686 3348 3963 2686
Size: 662 615 8340
Fragments arranged by size:
8340 662 615
PmeI GTTT'AAAC
Cuts at: 6616 6616
Size: 9617
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8756 8788 8756
Size: 32 9585
PpuMI rG'GwC_Cy
Cuts at: 5283 6300 5283
Size: 1017 8600
PsiI TTA'TAA
Cuts at: 6398 6398
Size: 9617
PstI C_TGCA'G
Cuts at: 5190 6664 7349 5190
Size: 1474 685 7458
Fragments arranged by size:
7458 1474 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9617
PvuII CAG'CTG
Cuts at: 271 3541 5191 7042 7402 271
Size: 3270 1650 1851 360 2486
Fragments arranged by size:
3270 2486 1851 1650 360
RsrII CG'GwC_CG
Cuts at: 7812 7812
Size: 9617
SacI G_AGCT'C
Cuts at: 2991 6631 2991
Size: 3640 5977
SalI G'TCGA_C
Cuts at: 2708 6654 2708
Size: 3946 5671
SapI GCTCTTCn'nnn_
Cuts at: 1447 3929 4136 7640 7850 1447
Size: 2482 207 3504 210 3214
Fragments arranged by size:
3504 3214 2482 210 207
SbfI CC_TGCA'GG
Cuts at: 6664 6664
Size: 9617
ScaI AGT'ACT
Cuts at: 9134 9134
Size: 9617
SexAI A'CCwGG_T
Cuts at: 1004 1004
Size: 9617
SfcI C'TryA_G
Cuts at: 33 4725 5186 6660 7345 8338 8529 33
Size: 4692 461 1474 685 993 191 1121
Fragments arranged by size:
4692 1474 1121 993 685 461 191
SfoI GGC'GCC
Cuts at: 2401 7049 7297 2401
Size: 4648 248 4721
Fragments arranged by size:
4721 4648 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9617
SmaI CCC'GGG
Cuts at: 6639 6639
Size: 9617
SmlI C'TyrA_G
Cuts at: 873 2502 2780 3828 5375 5386 5444 6205 6772
Size: 1629 278 1048 1547 11 58 761 567
Cuts at: 6772 7687 8179 8441 873
Size: 915 492 262 2049
Fragments arranged by size:
2049 1629 1547 1048 915 761 567 492
278 262 58 11
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9617
SphI G_CATG'C
Cuts at: 3776 6670 7170 3776
Size: 2894 500 6223
Fragments arranged by size:
6223 2894 500
SspI AAT'ATT
Cuts at: 3444 5040 3444
Size: 1596 8021
StyI C'CwwG_G
Cuts at: 107 695 808 3947 4035 6296 6751 7105 107
Size: 588 113 3139 88 2261 455 354 2619
Fragments arranged by size:
3139 2619 2261 588 455 354 113 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 344 414 1559 2530 3493 3834 3972 4580 7200
Size: 70 1145 971 963 341 138 608 2620
Cuts at: 7200 7333 7792 8001 344
Size: 133 459 209 1960
Fragments arranged by size:
2620 1960 1145 971 963 608 459 341
209 138 133 70
TatI w'GTAC_w
Cuts at: 669 712 1720 3370 3510 4187 9132 669
Size: 43 1008 1650 140 677 4945 1154
Fragments arranged by size:
4945 1650 1154 1008 677 140 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3945 3977 9134 9166 3945
Size: 32 5157 32 4396
Fragments arranged by size:
5157 4396 32 32
Tth111I GACn'n_nGTC
Cuts at: 7414 7414
Size: 9617
XbaI T'CTAG_A
Cuts at: 909 3000 5523 6486 6648 909
Size: 2091 2523 963 162 3878
Fragments arranged by size:
3878 2523 2091 963 162
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 334 446 6020 7112 334
Size: 112 5574 1092 2839
Fragments arranged by size:
5574 2839 1092 112
XhoI C'TCGA_G
Cuts at: 5444 5444
Size: 9617
XmaI C'CCGG_G
Cuts at: 6637 6637
Size: 9617
XmnI GAAnn'nnTTC
Cuts at: 1890 4623 5942 1890
Size: 2733 1319 5565
Fragments arranged by size:
5565 2733 1319
ZraI GAC'GTC
Cuts at: 2698 2698
Size: 9617
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PflMI PfoI PmeI PpiI PpuMI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AloI ApaI AscI AvrII BaeI
BglI BmgBI BmtI BsiWI BsrGI BssHII BstZ17I ClaI
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
PacI PasI PmlI PshAI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI StuI SwaI