Restriction map for
pF1KE2413
(Circular) (Six-base) MAPSORT of: pF1KE2413.seq Check: 2524 from: 1 to: 9044
pF1KE2413 9044 bp
With 182 enzymes: SgfI *
July 23, 2012 18:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 483 3199 5502 483
Size: 2716 2303 4025
Fragments arranged by size:
4025 2716 2303
AatII G_ACGT'C
Cuts at: 1458 1458
Size: 9044
AccI GT'mk_AC
Cuts at: 3245 3464 6082 3245
Size: 219 2618 6207
Fragments arranged by size:
6207 2618 219
Acc65I G'GTAC_C
Cuts at: 2634 5463 5696 6060 2634
Size: 2829 233 364 5618
Fragments arranged by size:
5618 2829 364 233
AclI AA'CG_TT
Cuts at: 8884 8884
Size: 9044
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 1415 3944 4153 4732 4942 6869 7301 8048
Size: 1275 2529 209 579 210 1927 432 747
Cuts at: 8048 8316 140
Size: 268 868
Fragments arranged by size:
2529 1927 1275 868 747 579 432 268
210 209
AflIII A'CryG_T
Cuts at: 1733 2192 2360 3260 3872 6396 7500 8477 1733
Size: 459 168 900 612 2524 1104 977 2300
Fragments arranged by size:
2524 2300 1104 977 900 612 459 168
AgeI A'CCGG_T
Cuts at: 1531 7432 1531
Size: 5901 3143
AhdI GACnn_n'nnGTC
Cuts at: 5973 5973
Size: 9044
AleI CACnn'nnGTG
Cuts at: 1936 1936
Size: 9044
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 342 1990 3178 5897 342
Size: 1648 1188 2719 3489
Fragments arranged by size:
3489 2719 1648 1188
AlwNI CAG_nnn'CTG
Cuts at: 172 1628 2021 2229 2410 4755 4800 5529 6349
Size: 1456 393 208 181 2345 45 729 820
Cuts at: 6349 7916 172
Size: 1567 1300
Fragments arranged by size:
2345 1567 1456 1300 820 729 393 208
181 45
ApaI G_GGCC'C
Cuts at: 203 658 1025 1861 4474 5192 5600 203
Size: 455 367 836 2613 718 408 3647
Fragments arranged by size:
3647 2613 836 718 455 408 367
ApaLI G'TGCA_C
Cuts at: 981 7814 981
Size: 6833 2211
ApoI r'AATT_y
Cuts at: 3181 4489 4852 4891 5307 5589 6048 8397 8653
Size: 1308 363 39 416 282 459 2349 256
Cuts at: 8653 3181
Size: 3572
Fragments arranged by size:
3572 2349 1308 459 416 363 282 256
39
AseI AT'TA_AT
Cuts at: 20 20
Size: 9044
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9044
AvaI C'yCGr_G
Cuts at: 196 314 768 874 5843 6064 196
Size: 118 454 106 4969 221 3176
Fragments arranged by size:
4969 3176 454 221 118 106
AvrII C'CTAG_G
Cuts at: 240 240
Size: 9044
BamHI G'GATC_C
Cuts at: 919 4043 5248 6069 919
Size: 3124 1205 821 3894
Fragments arranged by size:
3894 3124 1205 821
BanI G'GyrC_C
Cuts at: 810 1948 2475 2634 5456 5463 5569 5696 6060
Size: 1138 527 159 2822 7 106 127 364
Cuts at: 6060 6474 6722 6757 810
Size: 414 248 35 3097
Fragments arranged by size:
3097 2822 1138 527 414 364 248 159
127 106 35 7
BanII G_rGCy'C
Cuts at: 149 203 658 875 1025 1581 1861 2796 3235
Size: 54 455 217 150 556 280 935 439
Cuts at: 3235 3958 4474 5131 5192 5280 5439 5600 5610
Size: 723 516 657 61 88 159 161 10
Cuts at: 5610 6058 7088 149
Size: 448 1030 2105
Fragments arranged by size:
2105 1030 935 723 657 556 516 455
448 439 280 217 161 159 150 88
61 54 10
BbeI G_GCGC'C
Cuts at: 1952 6478 6726 1952
Size: 4526 248 4270
Fragments arranged by size:
4526 4270 248
BbsI GAAGACnn'nnnn_
Cuts at: 953 1068 1310 1716 1868 2715 3783 953
Size: 115 242 406 152 847 1068 6214
Fragments arranged by size:
6214 1068 847 406 242 152 115
BbvCI CC'TCA_GC
Cuts at: 2668 2686 2755 2777 5115 5633 2668
Size: 18 69 22 2338 518 6079
Fragments arranged by size:
6079 2338 518 69 22 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 341 351 375 385 3385 3419 3631 3665 4601
Size: 10 24 10 3000 34 212 34 936
Cuts at: 4601 4635 341
Size: 34 4750
Fragments arranged by size:
4750 3000 936 212 34 34 34 24
10 10
BciVI GTATCCnnnnn_n'
Cuts at: 4582 6942 7703 9044 4582
Size: 2360 761 1341 4582
Fragments arranged by size:
4582 2360 1341 761
BclI T'GATC_A
Cuts at: 4400 4424 6564 4400
Size: 24 2140 6880
Fragments arranged by size:
6880 2140 24
BfrBI ATG'CAT
Cuts at: 3400 3400
Size: 9044
BglI GCCn_nnn'nGGC
Cuts at: 163 750 1409 1773 3337 3604 3947 4960 163
Size: 587 659 364 1564 267 343 1013 4247
Fragments arranged by size:
4247 1564 1013 659 587 364 343 267
BglII A'GATC_T
Cuts at: 3443 4775 6559 8596 3443
Size: 1332 1784 2037 3891
Fragments arranged by size:
3891 2037 1784 1332
BlpI GC'TnA_GC
Cuts at: 6156 6156
Size: 9044
Bme1580I G_kGCm'C
Cuts at: 196 203 303 321 658 985 1025 1668 1861
Size: 7 100 18 337 327 40 643 193
Cuts at: 1861 3534 4474 4606 5192 5240 5459 5600 6669
Size: 1673 940 132 586 48 219 141 1069
Cuts at: 6669 6762 7818 196
Size: 93 1056 1422
Fragments arranged by size:
1673 1422 1069 1056 940 643 586 337
327 219 193 141 132 100 93 48
40 18 7
BmgBI CAC'GTC
Cuts at: 905 3261 905
Size: 2356 6688
BmrI ACTGGGnnnn_n'
Cuts at: 143 1713 2031 5186 5225 6256 6337 6672 8816
Size: 1570 318 3155 39 1031 81 335 2144
Cuts at: 8816 143
Size: 371
Fragments arranged by size:
3155 2144 1570 1031 371 335 318 81
39
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 480 1380 3984 7411 480
Size: 900 2604 3427 2113
Fragments arranged by size:
3427 2604 2113 900
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 667 2967 4756 5205 508
Size: 159 2300 1789 449 4347
Fragments arranged by size:
4347 2300 1789 449 159
Bpu10I CC'TnA_GC
Cuts at: 251 2668 2686 2755 2777 5115 5609 5633 251
Size: 2417 18 69 22 2338 494 24 3662
Fragments arranged by size:
3662 2417 2338 494 69 24 22 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 1319 3049 3203 3586 6220 7099 7591 7889
Size: 796 1730 154 383 2634 879 492 298
Cuts at: 7889 523
Size: 1678
Fragments arranged by size:
2634 1730 1678 879 796 492 383 298
154
BsaI GGTCTCn'nnnn_
Cuts at: 1540 1921 5109 5840 8748 1540
Size: 381 3188 731 2908 1836
Fragments arranged by size:
3188 2908 1836 731 381
BsaAI yAC'GTr
Cuts at: 504 5745 6397 504
Size: 5241 652 3151
Fragments arranged by size:
5241 3151 652
BsaBI GATnn'nnATC
Cuts at: 6 3496 4765 6558 8595 6
Size: 3490 1269 1793 2037 455
Fragments arranged by size:
3490 2037 1793 1269 455
BsaHI Gr'CG_yC
Cuts at: 674 1214 1455 1949 4931 6475 6723 7425 8921
Size: 540 241 494 2982 1544 248 702 1496
Cuts at: 8921 674
Size: 797
Fragments arranged by size:
2982 1544 1496 797 702 540 494 248
241
BsaWI w'CCGG_w
Cuts at: 1531 3098 4700 6233 6754 7432 7706 7853 8264
Size: 1567 1602 1533 521 678 274 147 411
Cuts at: 8264 1531
Size: 2311
Fragments arranged by size:
2311 1602 1567 1533 678 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2890 2920 2890
Size: 30 9014
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 952 1582 1585 1984 2083 2200 2515 2548 2551
Size: 630 3 399 99 117 315 33 3
Cuts at: 2551 2554 2557 2560 4846 4849 5041 5044 5074
Size: 3 3 3 2286 3 192 3 30
Cuts at: 5074 5986 6013 952
Size: 912 27 3983
Fragments arranged by size:
3983 2286 912 630 399 315 192 117
99 33 30 27 3 3 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 204 302 571 1518 3608 5108 5767 6469 7227
Size: 98 269 947 2090 1500 659 702 758
Cuts at: 7227 7804 8300 204
Size: 577 496 948
Fragments arranged by size:
2090 1500 948 947 758 702 659 577
496 269 98
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 760 805 2606 2881 3097 3177 3184 3359 5725
Size: 45 1801 275 216 80 7 175 2366
Cuts at: 5725 760
Size: 4079
Fragments arranged by size:
4079 2366 1801 275 216 175 80 45
7
BsiEI CG_ry'CG
Cuts at: 104 6586 6632 7840 104
Size: 6482 46 1208 1308
Fragments arranged by size:
6482 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 539 566 788 842 985 1115 1757 1979 3824
Size: 27 222 54 143 130 642 222 1845
Cuts at: 3824 3958 5413 5439 6058 6836 7026 7818 539
Size: 134 1455 26 619 778 190 792 1765
Fragments arranged by size:
1845 1765 1455 792 778 642 619 222
222 190 143 134 130 54 27 26
BsmI GAATG_Cn'
Cuts at: 899 3229 899
Size: 2330 6714
BsmBI CGTCTCn'nnnn_
Cuts at: 523 3251 4560 6005 523
Size: 2728 1309 1445 3562
Fragments arranged by size:
3562 2728 1445 1309
Bsp1286I G_dGCh'C
Cuts at: 149 196 203 303 321 539 566 658 788
Size: 47 7 100 18 218 27 92 130
Cuts at: 788 842 875 985 1025 1115 1581 1668 1757
Size: 54 33 110 40 90 466 87 89
Cuts at: 1757 1861 1979 2796 3235 3534 3824 3958 4474
Size: 104 118 817 439 299 290 134 516
Cuts at: 4474 4606 5131 5192 5240 5280 5413 5439 5459
Size: 132 525 61 48 40 133 26 20
Cuts at: 5459 5600 5610 6058 6669 6762 6836 7026 7088
Size: 141 10 448 611 93 74 190 62
Cuts at: 7088 7818 149
Size: 730 1375
Fragments arranged by size:
1375 817 730 611 525 516 466 448
439 299 290 218 190 141 134 133
132 130 118 110 104 100 93 92
90 89 87 74 62 61 54 48
47 40 40 33 27 26 20 18
10 7
BspEI T'CCGG_A
Cuts at: 3098 4700 8264 3098
Size: 1602 3564 3878
Fragments arranged by size:
3878 3564 1602
BspHI T'CATG_A
Cuts at: 797 1613 3116 3488 8220 797
Size: 816 1503 372 4732 1621
Fragments arranged by size:
4732 1621 1503 816 372
BspMI ACCTGCnnnn'nnnn_
Cuts at: 483 1316 3199 5488 5502 5942 6094 6308 6610
Size: 833 1883 2289 14 440 152 214 302
Cuts at: 6610 6991 483
Size: 381 2536
Fragments arranged by size:
2536 2289 1883 833 440 381 302 214
152 14
BsrBI CCG'CTC
Cuts at: 50 3285 7336 7390 8874 9039 50
Size: 3235 4051 54 1484 165 55
Fragments arranged by size:
4051 3235 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 594 824 2372 2445 3476 4051 4118 4643 6956
Size: 230 1548 73 1031 575 67 525 2313
Cuts at: 6956 594
Size: 2682
Fragments arranged by size:
2682 2313 1548 1031 575 525 230 73
67
BsrFI r'CCGG_y
Cuts at: 1531 7042 7223 7432 1531
Size: 5511 181 209 3143
Fragments arranged by size:
5511 3143 209 181
BsrGI T'GTAC_A
Cuts at: 1591 1591
Size: 9044
BssSI C'ACGA_G
Cuts at: 835 7315 7673 835
Size: 6480 358 2206
Fragments arranged by size:
6480 2206 358
BstAPI GCAn_nnn'nTGC
Cuts at: 783 2410 3075 6421 783
Size: 1627 665 3346 3406
Fragments arranged by size:
3406 3346 1627 665
BstBI TT'CG_AA
Cuts at: 7405 7405
Size: 9044
BstEII G'GTnAC_C
Cuts at: 308 2010 4839 308
Size: 1702 2829 4513
Fragments arranged by size:
4513 2829 1702
BstXI CCAn_nnnn'nTGG
Cuts at: 426 1938 2379 2997 426
Size: 1512 441 618 6473
Fragments arranged by size:
6473 1512 618 441
BstYI r'GATC_y
Cuts at: 1 919 1784 2000 2225 2448 2937 3443 4043
Size: 918 865 216 225 223 489 506 600
Cuts at: 4043 4499 4775 5248 5707 6069 6536 6559 6894
Size: 456 276 473 459 362 467 23 335
Cuts at: 6894 7140 8152 8238 8250 8596 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 918 865 600 506 489 473 467
459 456 449 362 346 335 276 246
225 223 216 86 23 12
BstZ17I GTA'TAC
Cuts at: 3246 3246
Size: 9044
Bsu36I CC'TnA_GG
Cuts at: 455 1996 2645 455
Size: 1541 649 6854
Fragments arranged by size:
6854 1541 649
BtgI C'CryG_G
Cuts at: 1005 1091 1133 1758 2901 3414 3770 4844 5772
Size: 86 42 625 1143 513 356 1074 928
Cuts at: 5772 1005
Size: 4277
Fragments arranged by size:
4277 1143 1074 928 625 513 356 86
42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 932 1571 1728 2058 3816 4092 4344 6994 7175
Size: 639 157 330 1758 276 252 2650 181
Cuts at: 7175 7338 932
Size: 163 2638
Fragments arranged by size:
2650 2638 1758 639 330 276 252 181
163 157
BtsI GCAGTG_nn'
Cuts at: 231 296 470 829 1688 2408 2766 3085 3376
Size: 65 174 359 859 720 358 319 291
Cuts at: 3376 5719 5793 6287 231
Size: 2343 74 494 2988
Fragments arranged by size:
2988 2343 859 720 494 359 358 319
291 174 74 65
ClaI AT'CG_AT
Cuts at: 3395 3395
Size: 9044
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3918 3953 4671 4706 3918
Size: 35 718 35 8256
Fragments arranged by size:
8256 718 35 35
DraI TTT'AAA
Cuts at: 6043 6043
Size: 9044
DraIII CAC_nnn'GTG
Cuts at: 783 4614 783
Size: 3831 5213
DrdI GACnn_nn'nnGTC
Cuts at: 6750 7608 6750
Size: 858 8186
EaeI y'GGCC_r
Cuts at: 293 338 664 742 1015 1417 1427 1583 2390
Size: 45 326 78 273 402 10 156 807
Cuts at: 2390 2771 3092 3939 5867 6629 6803 7194 7221
Size: 381 321 847 1928 762 174 391 27
Cuts at: 7221 8566 293
Size: 1345 771
Fragments arranged by size:
1928 1345 847 807 771 762 402 391
381 326 321 273 174 156 78 45
27 10
EagI C'GGCC_G
Cuts at: 6629 6629
Size: 9044
EarI CTCTTCn'nnn_
Cuts at: 328 847 1337 1391 2101 2107 2335 2428 2492
Size: 519 490 54 710 6 228 93 64
Cuts at: 2492 2549 2558 2567 2573 3175 3808 4003 4106
Size: 57 9 9 6 602 633 195 103
Cuts at: 4106 4172 4325 4953 5033 5669 5773 7067 7277
Size: 66 153 628 80 636 104 1294 210
Cuts at: 7277 328
Size: 2095
Fragments arranged by size:
2095 1294 710 636 633 628 602 519
490 228 210 195 153 104 103 93
80 66 64 57 54 9 9 6
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1356 1630 3607 4914 4941 7252 7562 7708 8854
Size: 274 1977 1307 27 2311 310 146 1146
Cuts at: 8854 1356
Size: 1546
Fragments arranged by size:
2311 1977 1546 1307 1146 310 274 146
27
EcoICRI GAG'CTC
Cuts at: 3956 5437 6056 3956
Size: 1481 619 6944
Fragments arranged by size:
6944 1481 619
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 508 667 1415 2967 3944 4153 4732 4756
Size: 368 159 748 1552 977 209 579 24
Cuts at: 4756 4942 5205 6869 7301 8048 8316 140
Size: 186 263 1664 432 747 268 868
Fragments arranged by size:
1664 1552 977 868 748 747 579 432
368 268 263 209 186 159 24
EcoNI CCTnn'n_nnAGG
Cuts at: 490 490
Size: 9044
EcoO109I rG'GnC_Cy
Cuts at: 169 200 245 654 655 765 942 1021 1184
Size: 31 45 409 1 110 177 79 163
Cuts at: 1184 1359 1656 1858 2205 2781 2888 4471 4990
Size: 175 297 202 347 576 107 1583 519
Cuts at: 4990 5007 5596 6183 169
Size: 17 589 587 3030
Fragments arranged by size:
3030 1583 589 587 576 519 409 347
297 202 177 175 163 110 107 79
45 31 17 1
EcoRI G'AATT_C
Cuts at: 4489 6048 4489
Size: 1559 7485
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2172 3252 3870 5529 7063 2172
Size: 1080 618 1659 1534 4153
Fragments arranged by size:
4153 1659 1534 1080 618
FspI TGC'GCA
Cuts at: 5725 6825 5725
Size: 1100 7944
HaeII r_GCGC'y
Cuts at: 578 1014 1952 2657 2990 5538 6478 6726 7748
Size: 436 938 705 333 2548 940 248 1022
Cuts at: 7748 8727 578
Size: 979 895
Fragments arranged by size:
2548 1022 979 940 938 895 705 436
333 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 428 460 931 963 4294 4326 4392 4424 5164
Size: 32 471 32 3331 32 66 32 740
Cuts at: 5164 5196 5959 5991 6886 6916 6918 6948 428
Size: 32 763 32 895 30 2 30 2524
Fragments arranged by size:
3331 2524 895 763 740 471 66 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1739 3297 6083 1739
Size: 1558 2786 4700
Fragments arranged by size:
4700 2786 1558
HindIII A'AGCT_T
Cuts at: 4021 4021
Size: 9044
KasI G'GCGC_C
Cuts at: 1948 6474 6722 1948
Size: 4526 248 4270
Fragments arranged by size:
4526 4270 248
KpnI G_GTAC'C
Cuts at: 2638 5467 5700 6064 2638
Size: 2829 233 364 5618
Fragments arranged by size:
5618 2829 364 233
MluI A'CGCG_T
Cuts at: 8477 8477
Size: 9044
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 92 491 947 2915 5276 5482 7715 7899 92
Size: 399 456 1968 2361 206 2233 184 1237
Fragments arranged by size:
2361 2233 1968 1237 456 399 206 184
MscI TGG'CCA
Cuts at: 295 340 1017 1585 2392 3094 3941 5869 6805
Size: 45 677 568 807 702 847 1928 936
Cuts at: 6805 295
Size: 2534
Fragments arranged by size:
2534 1928 936 847 807 702 677 568
45
MslI CAynn'nnrTG
Cuts at: 619 1936 1972 2377 2717 2995 3160 3502 4111
Size: 1317 36 405 340 278 165 342 609
Cuts at: 4111 4141 4147 4484 4547 7033 7160 619
Size: 30 6 337 63 2486 127 2503
Fragments arranged by size:
2503 2486 1317 609 405 342 340 337
278 165 127 63 36 30 6
MspA1I CmG'CkG
Cuts at: 208 306 816 1007 1281 1425 1589 2136 2685
Size: 98 510 191 274 144 164 547 549
Cuts at: 2685 2776 2903 5637 5661 5686 6155 6469 6829
Size: 91 127 2734 24 25 469 314 360
Cuts at: 6829 7842 8087 208
Size: 1013 245 1165
Fragments arranged by size:
2734 1165 1013 549 547 510 469 360
314 274 245 191 164 144 127 98
91 25 24
NaeI GCC'GGC
Cuts at: 7225 7225
Size: 9044
NarI GG'CG_CC
Cuts at: 1949 6475 6723 1949
Size: 4526 248 4270
Fragments arranged by size:
4526 4270 248
NcoI C'CATG_G
Cuts at: 1091 1133 3770 5772 1091
Size: 42 2637 2002 4363
Fragments arranged by size:
4363 2637 2002 42
NdeI CA'TA_TG
Cuts at: 2277 3402 2277
Size: 1125 7919
NgoMIV G'CCGG_C
Cuts at: 7223 7223
Size: 9044
NsiI A_TGCA'T
Cuts at: 3402 3402
Size: 9044
NspI r_CATG'y
Cuts at: 2133 3073 4146 4647 6097 6597 7504 2133
Size: 940 1073 501 1450 500 907 3673
Fragments arranged by size:
3673 1450 1073 940 907 501 500
PasI CC'CwG_GG
Cuts at: 432 1151 3534 4519 432
Size: 719 2383 985 4957
Fragments arranged by size:
4957 2383 985 719
PciI A'CATG_T
Cuts at: 7500 7500
Size: 9044
PflMI CCAn_nnn'nTGG
Cuts at: 160 486 2021 2817 4457 160
Size: 326 1535 796 1640 4747
Fragments arranged by size:
4747 1640 1535 796 326
PfoI T'CCnGG_A
Cuts at: 1787 3278 4287 4874 5131 1787
Size: 1491 1009 587 257 5700
Fragments arranged by size:
5700 1491 1009 587 257
PmeI GTTT'AAAC
Cuts at: 6043 6043
Size: 9044
PmlI CAC'GTG
Cuts at: 5745 5745
Size: 9044
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5940 5972 8183 8215 5940
Size: 32 2211 32 6769
Fragments arranged by size:
6769 2211 32 32
PpuMI rG'GwC_Cy
Cuts at: 245 765 942 1184 1359 1656 2781 4990 245
Size: 520 177 242 175 297 1125 2209 4299
Fragments arranged by size:
4299 2209 1125 520 297 242 177 175
PshAI GACnn'nnGTC
Cuts at: 4988 4988
Size: 9044
PspOMI G'GGCC_C
Cuts at: 199 654 1021 1857 4470 5188 5596 199
Size: 455 367 836 2613 718 408 3647
Fragments arranged by size:
3647 2613 836 718 455 408 367
PspXI vC'TCGA_Gb
Cuts at: 874 874
Size: 9044
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3679 3711 3679
Size: 32 9012
PstI C_TGCA'G
Cuts at: 2609 4982 5499 5534 6091 6776 2609
Size: 2373 517 35 557 685 4877
Fragments arranged by size:
4877 2373 685 557 517 35
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9044
PvuII CAG'CTG
Cuts at: 208 1425 1589 2136 6469 6829 208
Size: 1217 164 547 4333 360 2423
Fragments arranged by size:
4333 2423 1217 547 360 164
RsrII CG'GwC_CG
Cuts at: 7239 7239
Size: 9044
SacI G_AGCT'C
Cuts at: 3958 5439 6058 3958
Size: 1481 619 6944
Fragments arranged by size:
6944 1481 619
SacII CC_GC'GG
Cuts at: 1008 2904 1008
Size: 1896 7148
SalI G'TCGA_C
Cuts at: 6081 6081
Size: 9044
SanDI GG'GwC_CC
Cuts at: 245 765 245
Size: 520 8524
SapI GCTCTTCn'nnn_
Cuts at: 847 1337 2428 3175 4106 4172 7067 7277 847
Size: 490 1091 747 931 66 2895 210 2614
Fragments arranged by size:
2895 2614 1091 931 747 490 210 66
SbfI CC_TGCA'GG
Cuts at: 2609 4982 5499 6091 2609
Size: 2373 517 592 5562
Fragments arranged by size:
5562 2373 592 517
ScaI AGT'ACT
Cuts at: 3017 3026 8561 3017
Size: 9 5535 3500
Fragments arranged by size:
5535 3500 9
SexAI A'CCwGG_T
Cuts at: 5459 5459
Size: 9044
SfcI C'TryA_G
Cuts at: 33 2605 4978 5495 5530 5961 6087 6772 7765
Size: 2572 2373 517 35 431 126 685 993
Cuts at: 7765 7956 33
Size: 191 1121
Fragments arranged by size:
2572 2373 1121 993 685 517 431 191
126 35
SfiI GGCCn_nnn'nGGCC
Cuts at: 1409 1409
Size: 9044
SfoI GGC'GCC
Cuts at: 1950 6476 6724 1950
Size: 4526 248 4270
Fragments arranged by size:
4526 4270 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9044
SmaI CCC'GGG
Cuts at: 198 6066 198
Size: 5868 3176
SmlI C'TyrA_G
Cuts at: 538 874 1334 3064 3218 3601 5843 6199 7114
Size: 336 460 1730 154 383 2242 356 915
Cuts at: 7114 7606 7868 538
Size: 492 262 1714
Fragments arranged by size:
2242 1730 1714 915 492 460 383 356
336 262 154
SnaBI TAC'GTA
Cuts at: 504 504
Size: 9044
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9044
SphI G_CATG'C
Cuts at: 2133 4146 6097 6597 2133
Size: 2013 1951 500 4580
Fragments arranged by size:
4580 2013 1951 500
StuI AGG'CCT
Cuts at: 239 2471 4084 239
Size: 2232 1613 5199
Fragments arranged by size:
5199 2232 1613
StyI C'CwwG_G
Cuts at: 240 885 1091 1133 1235 1380 1551 1934 1961
Size: 645 206 42 102 145 171 383 27
Cuts at: 1961 2414 2621 3056 3770 3945 4526 5318 5772
Size: 453 207 435 714 175 581 792 454
Cuts at: 5772 6178 6532 240
Size: 406 354 2752
Fragments arranged by size:
2752 792 714 645 581 454 453 435
406 383 354 207 206 175 171 145
102 42 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1120 1143 1795 5272 6627 6760 7219 7428 1120
Size: 23 652 3477 1355 133 459 209 2736
Fragments arranged by size:
3477 2736 1355 652 459 209 133 23
TatI w'GTAC_w
Cuts at: 1157 1591 3015 3024 8559 1157
Size: 434 1424 9 5535 1642
Fragments arranged by size:
5535 1642 1424 434 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2291 2323 2890 2922 8561 8593 2291
Size: 32 567 32 5639 32 2742
Fragments arranged by size:
5639 2742 567 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3319 6841 3319
Size: 3522 5522
XbaI T'CTAG_A
Cuts at: 6075 6075
Size: 9044
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 483 1959 2628 2736 2853 5934 6539 483
Size: 1476 669 108 117 3081 605 2988
Fragments arranged by size:
3081 2988 1476 669 605 117 108
XhoI C'TCGA_G
Cuts at: 874 5843 874
Size: 4969 4075
XmaI C'CCGG_G
Cuts at: 196 6064 196
Size: 5868 3176
XmnI GAAnn'nnTTC
Cuts at: 385 3499 385
Size: 3114 5930
ZraI GAC'GTC
Cuts at: 1456 1456
Size: 9044
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AloI AscI BaeI BmtI BsiWI BssHII
EcoRV FseI FspAI HpaI MfeI NheI NotI NruI
PacI PsiI SgrAI SrfI SspI SwaI