Restriction map for
pF1KE2416
(Circular) (Six-base) MAPSORT of: pF1KE2416.seq Check: 9606 from: 1 to: 5030
pF1KE2416 5030 bp
With 182 enzymes: SgfI *
June 11, 2014 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 298 1207 1820 298
Size: 909 613 3508
Fragments arranged by size:
3508 909 613
AccI GT'mk_AC
Cuts at: 2068 2068
Size: 5030
Acc65I G'GTAC_C
Cuts at: 1571 2046 1571
Size: 475 4555
AclI AA'CG_TT
Cuts at: 4870 4870
Size: 5030
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1885 2855 3287 4034 4302 1885
Size: 970 432 747 268 2613
Fragments arranged by size:
2613 970 747 432 268
AfeI AGC'GCT
Cuts at: 1515 1515
Size: 5030
AflIII A'CryG_T
Cuts at: 2382 3486 4463 2382
Size: 1104 977 2949
Fragments arranged by size:
2949 1104 977
AgeI A'CCGG_T
Cuts at: 3418 3418
Size: 5030
AleI CACnn'nnGTG
Cuts at: 709 709
Size: 5030
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1609 1609
Size: 5030
AlwNI CAG_nnn'CTG
Cuts at: 2335 3902 2335
Size: 1567 3463
ApaI G_GGCC'C
Cuts at: 268 1758 268
Size: 1490 3540
ApaLI G'TGCA_C
Cuts at: 1194 3800 1194
Size: 2606 2424
ApoI r'AATT_y
Cuts at: 2034 4383 4639 2034
Size: 2349 256 2425
Fragments arranged by size:
2425 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5030
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5030
AvaI C'yCGr_G
Cuts at: 231 1554 1804 2050 231
Size: 1323 250 246 3211
Fragments arranged by size:
3211 1323 250 246
BamHI G'GATC_C
Cuts at: 227 566 1177 2055 227
Size: 339 611 878 3202
Fragments arranged by size:
3202 878 611 339
BanI G'GyrC_C
Cuts at: 400 417 688 1022 1333 1456 1571 1578 1808
Size: 17 271 334 311 123 115 7 230
Cuts at: 1808 2046 2460 2708 2743 400
Size: 238 414 248 35 2687
Fragments arranged by size:
2687 414 334 311 271 248 238 230
123 115 35 17 7
BanII G_rGCy'C
Cuts at: 268 595 655 679 926 1163 1758 1922 2044
Size: 327 60 24 247 237 595 164 122
Cuts at: 2044 3074 268
Size: 1030 2224
Fragments arranged by size:
2224 1030 595 327 247 237 164 122
60 24
BbeI G_GCGC'C
Cuts at: 404 1337 2464 2712 404
Size: 933 1127 248 2722
Fragments arranged by size:
2722 1127 933 248
BbsI GAAGACnn'nnnn_
Cuts at: 1941 1941
Size: 5030
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1329 1363 1731 1765 1329
Size: 34 368 34 4594
Fragments arranged by size:
4594 368 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1570 2928 3689 5030 1570
Size: 1358 761 1341 1570
Fragments arranged by size:
1570 1358 1341 761
BclI T'GATC_A
Cuts at: 1409 2550 1409
Size: 1141 3889
BglI GCCn_nnn'nGGC
Cuts at: 340 398 1477 1762 340
Size: 58 1079 285 3608
Fragments arranged by size:
3608 1079 285 58
BglII A'GATC_T
Cuts at: 1016 2545 4582 1016
Size: 1529 2037 1464
Fragments arranged by size:
2037 1529 1464
BlpI GC'TnA_GC
Cuts at: 281 469 2142 281
Size: 188 1673 3169
Fragments arranged by size:
3169 1673 188
Bme1580I G_kGCm'C
Cuts at: 206 268 1198 1583 1744 1758 1811 2655 2748
Size: 62 930 385 161 14 53 844 93
Cuts at: 2748 3804 206
Size: 1056 1432
Fragments arranged by size:
1432 1056 930 844 385 161 93 62
53 14
BmrI ACTGGGnnnn_n'
Cuts at: 151 1450 2242 2323 2658 4802 151
Size: 1299 792 81 335 2144 379
Fragments arranged by size:
2144 1299 792 379 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 669 1092 1941 3397 669
Size: 423 849 1456 2302
Fragments arranged by size:
2302 1456 849 423
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 367 512 579 664 838 1062 1975 331
Size: 36 145 67 85 174 224 913 3386
Fragments arranged by size:
3386 913 224 174 145 85 67 36
Bpu10I CC'TnA_GC
Cuts at: 1916 1916
Size: 5030
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 892 1222 2206 3085 3577 3875 397
Size: 495 330 984 879 492 298 1552
Fragments arranged by size:
1552 984 879 495 492 330 298
BsaI GGTCTCn'nnnn_
Cuts at: 633 4734 633
Size: 4101 929
BsaAI yAC'GTr
Cuts at: 201 2383 201
Size: 2182 2848
BsaBI GATnn'nnATC
Cuts at: 6 2544 4581 6
Size: 2538 2037 455
Fragments arranged by size:
2538 2037 455
BsaHI Gr'CG_yC
Cuts at: 401 1334 2461 2709 3411 4907 401
Size: 933 1127 248 702 1496 524
Fragments arranged by size:
1496 1127 933 702 524 248
BsaWI w'CCGG_w
Cuts at: 1143 2219 2740 3418 3692 3839 4250 1143
Size: 1076 521 678 274 147 411 1923
Fragments arranged by size:
1923 1076 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 485 515 1018 1048 1492 1522 485
Size: 30 503 30 444 30 3993
Fragments arranged by size:
3993 503 444 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 364 367 621 1972 364
Size: 3 254 1351 3422
Fragments arranged by size:
3422 1351 254 3
BseYI C'CCAG_C
Cuts at: 129 205 256 1617 2455 3213 3790 4286 129
Size: 76 51 1361 838 758 577 496 873
Fragments arranged by size:
1361 873 838 758 577 496 76 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 823 1569 607
Size: 216 746 4068
Fragments arranged by size:
4068 746 216
BsiEI CG_ry'CG
Cuts at: 104 2572 2618 3826 104
Size: 2468 46 1208 1308
Fragments arranged by size:
2468 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 287 595 655 1198 1778 2044 2822 3012 3804
Size: 308 60 543 580 266 778 190 792
Cuts at: 3804 287
Size: 1513
Fragments arranged by size:
1513 792 778 580 543 308 266 190
60
Bsp1286I G_dGCh'C
Cuts at: 206 268 287 595 655 679 926 1163 1198
Size: 62 19 308 60 24 247 237 35
Cuts at: 1198 1583 1744 1758 1778 1811 1922 2044 2655
Size: 385 161 14 20 33 111 122 611
Cuts at: 2655 2748 2822 3012 3074 3804 206
Size: 93 74 190 62 730 1432
Fragments arranged by size:
1432 730 611 385 308 247 237 190
161 122 111 93 74 62 62 60
35 33 24 20 19 14
BspEI T'CCGG_A
Cuts at: 4250 4250
Size: 5030
BspHI T'CATG_A
Cuts at: 4206 4206
Size: 5030
BspMI ACCTGCnnnn'nnnn_
Cuts at: 298 845 911 1207 1713 1820 2080 2294 2596
Size: 547 66 296 506 107 260 214 302
Cuts at: 2596 2977 298
Size: 381 2351
Fragments arranged by size:
2351 547 506 381 302 296 260 214
107 66
BsrBI CCG'CTC
Cuts at: 50 1434 1925 3322 3376 4860 5025 50
Size: 1384 491 1397 54 1484 165 55
Fragments arranged by size:
1484 1397 1384 491 165 55 54
BsrDI GCAATG_nn'
Cuts at: 971 2942 971
Size: 1971 3059
BsrFI r'CCGG_y
Cuts at: 3028 3209 3418 3028
Size: 181 209 4640
Fragments arranged by size:
4640 209 181
BssSI C'ACGA_G
Cuts at: 3301 3659 3301
Size: 358 4672
BstAPI GCAn_nnn'nTGC
Cuts at: 177 1999 2407 177
Size: 1822 408 2800
Fragments arranged by size:
2800 1822 408
BstBI TT'CG_AA
Cuts at: 3391 3391
Size: 5030
BstEII G'GTnAC_C
Cuts at: 573 573
Size: 5030
BstXI CCAn_nnnn'nTGG
Cuts at: 490 546 672 1090 1329 1605 1617 490
Size: 56 126 418 239 276 12 3903
Fragments arranged by size:
3903 418 276 239 126 56 12
BstYI r'GATC_y
Cuts at: 1 227 566 1016 1177 2055 2522 2545 2880
Size: 226 339 450 161 878 467 23 335
Cuts at: 2880 3126 4138 4224 4236 4582 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 878 467 450 449 346 339 335
246 226 161 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 212 994 212
Size: 782 4248
BtgI C'CryG_G
Cuts at: 162 767 879 1103 1322 1466 1709 162
Size: 605 112 224 219 144 243 3483
Fragments arranged by size:
3483 605 243 224 219 144 112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2980 3161 3324 2980
Size: 181 163 4686
Fragments arranged by size:
4686 181 163
BtsI GCAGTG_nn'
Cuts at: 325 1993 2273 325
Size: 1668 280 3082
Fragments arranged by size:
3082 1668 280
DraI TTT'AAA
Cuts at: 2029 2029
Size: 5030
DrdI GACnn_nn'nnGTC
Cuts at: 2736 3594 2736
Size: 858 4172
EaeI y'GGCC_r
Cuts at: 548 885 1250 1379 1469 1487 1607 1631 2615
Size: 337 365 129 90 18 120 24 984
Cuts at: 2615 2789 3180 3207 4552 548
Size: 174 391 27 1345 1026
Fragments arranged by size:
1345 1026 984 391 365 337 174 129
120 90 27 24 18
EagI C'GGCC_G
Cuts at: 2615 2615
Size: 5030
EarI CTCTTCn'nnn_
Cuts at: 451 3053 3263 451
Size: 2602 210 2218
Fragments arranged by size:
2602 2218 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3238 3548 3694 4840 3238
Size: 310 146 1146 3428
Fragments arranged by size:
3428 1146 310 146
EcoICRI GAG'CTC
Cuts at: 593 653 2042 593
Size: 60 1389 3581
Fragments arranged by size:
3581 1389 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 367 512 579 664 838 1062 1885 1975
Size: 36 145 67 85 174 224 823 90
Cuts at: 1975 2855 3287 4034 4302 331
Size: 880 432 747 268 1059
Fragments arranged by size:
1059 880 823 747 432 268 224 174
145 90 85 67 36
EcoNI CCTnn'n_nnAGG
Cuts at: 349 721 1237 349
Size: 372 516 4142
Fragments arranged by size:
4142 516 372
EcoO109I rG'GnC_Cy
Cuts at: 134 216 236 265 306 1424 1754 1755 2169
Size: 82 20 29 41 1118 330 1 414
Cuts at: 2169 134
Size: 2995
Fragments arranged by size:
2995 1118 414 330 82 41 29 20
1
EcoRI G'AATT_C
Cuts at: 2034 2034
Size: 5030
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3049 3049
Size: 5030
FspI TGC'GCA
Cuts at: 2811 2811
Size: 5030
HaeII r_GCGC'y
Cuts at: 318 404 1337 1517 1618 2464 2712 3734 4713
Size: 86 933 180 101 846 248 1022 979
Cuts at: 4713 318
Size: 635
Fragments arranged by size:
1022 979 933 846 635 248 180 101
86
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1492 1524 1785 1817 2872 2902 2904 2934 1492
Size: 32 261 32 1055 30 2 30 3588
Fragments arranged by size:
3588 1055 261 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2069 2069
Size: 5030
KasI G'GCGC_C
Cuts at: 400 1333 2460 2708 400
Size: 933 1127 248 2722
Fragments arranged by size:
2722 1127 933 248
KpnI G_GTAC'C
Cuts at: 1575 2050 1575
Size: 475 4555
MluI A'CGCG_T
Cuts at: 4463 4463
Size: 5030
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 563 704 1472 1777 3701 3885 563
Size: 141 768 305 1924 184 1708
Fragments arranged by size:
1924 1708 768 305 184 141
MscI TGG'CCA
Cuts at: 1252 1489 1609 2791 1252
Size: 237 120 1182 3491
Fragments arranged by size:
3491 1182 237 120
MslI CAynn'nnrTG
Cuts at: 670 709 1054 1126 1327 1603 1708 2009 3019
Size: 39 345 72 201 276 105 301 1010
Cuts at: 3019 3146 670
Size: 127 2554
Fragments arranged by size:
2554 1010 345 301 276 201 127 105
72 39
MspA1I CmG'CkG
Cuts at: 486 948 1643 1935 2141 2455 2815 3828 4073
Size: 462 695 292 206 314 360 1013 245
Cuts at: 4073 486
Size: 1443
Fragments arranged by size:
1443 1013 695 462 360 314 292 245
206
NaeI GCC'GGC
Cuts at: 3211 3211
Size: 5030
NarI GG'CG_CC
Cuts at: 401 1334 2461 2709 401
Size: 933 1127 248 2722
Fragments arranged by size:
2722 1127 933 248
NcoI C'CATG_G
Cuts at: 879 1103 1322 1709 879
Size: 224 219 387 4200
Fragments arranged by size:
4200 387 224 219
NgoMIV G'CCGG_C
Cuts at: 3209 3209
Size: 5030
NspI r_CATG'y
Cuts at: 175 468 2083 2583 3490 175
Size: 293 1615 500 907 1715
Fragments arranged by size:
1715 1615 907 500 293
PasI CC'CwG_GG
Cuts at: 1496 1943 1496
Size: 447 4583
PciI A'CATG_T
Cuts at: 3486 3486
Size: 5030
PflMI CCAn_nnn'nTGG
Cuts at: 163 163
Size: 5030
PfoI T'CCnGG_A
Cuts at: 308 1952 308
Size: 1644 3386
PmeI GTTT'AAAC
Cuts at: 2029 2029
Size: 5030
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4169 4201 4169
Size: 32 4998
PpuMI rG'GwC_Cy
Cuts at: 134 216 236 306 134
Size: 82 20 70 4858
Fragments arranged by size:
4858 82 70 20
PspOMI G'GGCC_C
Cuts at: 264 1754 264
Size: 1490 3540
PstI C_TGCA'G
Cuts at: 1793 2077 2762 1793
Size: 284 685 4061
Fragments arranged by size:
4061 685 284
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5030
PvuII CAG'CTG
Cuts at: 486 948 2455 2815 486
Size: 462 1507 360 2701
Fragments arranged by size:
2701 1507 462 360
RsrII CG'GwC_CG
Cuts at: 3225 3225
Size: 5030
SacI G_AGCT'C
Cuts at: 595 655 2044 595
Size: 60 1389 3581
Fragments arranged by size:
3581 1389 60
SalI G'TCGA_C
Cuts at: 2067 2067
Size: 5030
SanDI GG'GwC_CC
Cuts at: 134 134
Size: 5030
SapI GCTCTTCn'nnn_
Cuts at: 3053 3263 3053
Size: 210 4820
SbfI CC_TGCA'GG
Cuts at: 2077 2077
Size: 5030
ScaI AGT'ACT
Cuts at: 4547 4547
Size: 5030
SfcI C'TryA_G
Cuts at: 33 1789 2073 2758 3751 3942 33
Size: 1756 284 685 993 191 1121
Fragments arranged by size:
1756 1121 993 685 284 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1762 1762
Size: 5030
SfoI GGC'GCC
Cuts at: 402 1335 2462 2710 402
Size: 933 1127 248 2722
Fragments arranged by size:
2722 1127 933 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5030
SmaI CCC'GGG
Cuts at: 233 1806 2052 233
Size: 1573 246 3211
Fragments arranged by size:
3211 1573 246
SmlI C'TyrA_G
Cuts at: 412 907 1237 2185 3100 3592 3854 412
Size: 495 330 948 915 492 262 1588
Fragments arranged by size:
1588 948 915 495 492 330 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5030
SphI G_CATG'C
Cuts at: 2083 2583 2083
Size: 500 4530
StuI AGG'CCT
Cuts at: 1914 1914
Size: 5030
StyI C'CwwG_G
Cuts at: 879 1089 1103 1253 1322 1610 1709 2164 2518
Size: 210 14 150 69 288 99 455 354
Cuts at: 2518 879
Size: 3391
Fragments arranged by size:
3391 455 354 288 210 150 99 69
14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1043 1387 2613 2746 3205 3414 1043
Size: 344 1226 133 459 209 2659
Fragments arranged by size:
2659 1226 459 344 209 133
TatI w'GTAC_w
Cuts at: 4545 4545
Size: 5030
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 143 175 485 517 1018 1050 1169 1201 4547
Size: 32 310 32 501 32 119 32 3346
Cuts at: 4547 4579 143
Size: 32 594
Fragments arranged by size:
3346 594 501 310 119 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2827 2827
Size: 5030
XbaI T'CTAG_A
Cuts at: 2061 2061
Size: 5030
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 342 708 1473 1950 2525 342
Size: 366 765 477 575 2847
Fragments arranged by size:
2847 765 575 477 366
XmaI C'CCGG_G
Cuts at: 231 1804 2050 231
Size: 1573 246 3211
Fragments arranged by size:
3211 1573 246
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BbvCI
BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI BssHII
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PmlI PshAI PsiI PspXI PsrI SacII SexAI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI