Restriction map for
pF1KE2421
(Circular) (Six-base) MAPSORT of: pF1KE2421.seq Check: 7004 from: 1 to: 10166
pF1KE2421 10166 bp
With 182 enzymes: SgfI *
October 17, 2012 17:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 136 136
Size: 10166
AccI GT'mk_AC
Cuts at: 1142 7204 1142
Size: 6062 4104
Acc65I G'GTAC_C
Cuts at: 1919 7182 1919
Size: 5263 4903
AclI AA'CG_TT
Cuts at: 10006 10006
Size: 10166
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 977 1349 1781 2302 2658 4726 5113 6266 7991
Size: 372 432 521 356 2068 387 1153 1725
Cuts at: 7991 8423 9170 9438 977
Size: 432 747 268 1705
Fragments arranged by size:
2068 1725 1705 1153 747 521 432 432
387 372 356 268
AflII C'TTAA_G
Cuts at: 427 427
Size: 10166
AflIII A'CryG_T
Cuts at: 5310 6045 7518 8622 9599 5310
Size: 735 1473 1104 977 5877
Fragments arranged by size:
5877 1473 1104 977 735
AgeI A'CCGG_T
Cuts at: 8554 8554
Size: 10166
AleI CACnn'nnGTG
Cuts at: 7039 7039
Size: 10166
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2450 6200 7130 2450
Size: 3750 930 5486
Fragments arranged by size:
5486 3750 930
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 424 456 1969 2001 424
Size: 32 1513 32 8589
Fragments arranged by size:
8589 1513 32 32
AlwNI CAG_nnn'CTG
Cuts at: 585 1711 2857 3817 7471 9038 585
Size: 1126 1146 960 3654 1567 1713
Fragments arranged by size:
3654 1713 1567 1146 1126 960
ApaI G_GGCC'C
Cuts at: 3415 6306 3415
Size: 2891 7275
ApaLI G'TGCA_C
Cuts at: 961 5704 7105 8936 961
Size: 4743 1401 1831 2191
Fragments arranged by size:
4743 2191 1831 1401
ApoI r'AATT_y
Cuts at: 322 669 745 1274 2397 3588 3631 3743 3828
Size: 347 76 529 1123 1191 43 112 85
Cuts at: 3828 4077 4566 5374 5788 6146 6516 7085 7170
Size: 249 489 808 414 358 370 569 85
Cuts at: 7170 9519 9775 322
Size: 2349 256 713
Fragments arranged by size:
2349 1191 1123 808 713 569 529 489
414 370 358 347 256 249 112 85
85 76 43
AseI AT'TA_AT
Cuts at: 20 3226 3775 20
Size: 3206 549 6411
Fragments arranged by size:
6411 3206 549
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10166
AvaI C'yCGr_G
Cuts at: 839 4209 7186 839
Size: 3370 2977 3819
Fragments arranged by size:
3819 3370 2977
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 401 434 6597 6630 401
Size: 33 6163 33 3937
Fragments arranged by size:
6163 3937 33 33
BamHI G'GATC_C
Cuts at: 1977 4851 7191 1977
Size: 2874 2340 4952
Fragments arranged by size:
4952 2874 2340
BanI G'GyrC_C
Cuts at: 946 1745 1919 2237 5634 6393 6427 7182 7596
Size: 799 174 318 3397 759 34 755 414
Cuts at: 7596 7844 7879 946
Size: 248 35 3233
Fragments arranged by size:
3397 3233 799 759 755 414 318 248
174 35 34
BanII G_rGCy'C
Cuts at: 122 690 3099 3415 5227 5331 5868 6306 6365
Size: 568 2409 316 1812 104 537 438 59
Cuts at: 6365 7180 8210 122
Size: 815 1030 2078
Fragments arranged by size:
2409 2078 1812 1030 815 568 537 438
316 104 59
BbeI G_GCGC'C
Cuts at: 7600 7848 7600
Size: 248 9918
BbsI GAAGACnn'nnnn_
Cuts at: 448 540 1202 2109 2165 2190 2473 6531 6877
Size: 92 662 907 56 25 283 4058 346
Cuts at: 6877 448
Size: 3737
Fragments arranged by size:
4058 3737 907 662 346 283 92 56
25
BbvCI CC'TCA_GC
Cuts at: 398 1412 398
Size: 1014 9152
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 6490 6524 90
Size: 34 6366 34 3732
Fragments arranged by size:
6366 3732 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 483 3482 3564 4944 5937 8064 8825 10166 483
Size: 2999 82 1380 993 2127 761 1341 483
Fragments arranged by size:
2999 2127 1380 1341 993 761 483 82
BclI T'GATC_A
Cuts at: 510 4527 7686 510
Size: 4017 3159 2990
Fragments arranged by size:
4017 3159 2990
BfrBI ATG'CAT
Cuts at: 4039 4039
Size: 10166
BglI GCCn_nnn'nGGC
Cuts at: 6170 6170
Size: 10166
BglII A'GATC_T
Cuts at: 1788 4318 7681 9718 1788
Size: 2530 3363 2037 2236
Fragments arranged by size:
3363 2530 2236 2037
BlpI GC'TnA_GC
Cuts at: 350 5998 7278 350
Size: 5648 1280 3238
Fragments arranged by size:
5648 3238 1280
Bme1580I G_kGCm'C
Cuts at: 949 965 3415 5708 6306 6432 7109 7791 7884
Size: 16 2450 2293 598 126 677 682 93
Cuts at: 7884 8940 949
Size: 1056 2175
Fragments arranged by size:
2450 2293 2175 1056 682 677 598 126
93 16
BmrI ACTGGGnnnn_n'
Cuts at: 1350 2761 3530 4173 7378 7459 7794 9938 1350
Size: 1411 769 643 3205 81 335 2144 1578
Fragments arranged by size:
3205 2144 1578 1411 769 643 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2576 4560 8533 2576
Size: 1984 3973 4209
Fragments arranged by size:
4209 3973 1984
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1982 2651 3884 6369 6429 6840 6936 1982
Size: 669 1233 2485 60 411 96 5212
Fragments arranged by size:
5212 2485 1233 669 411 96 60
Bpu10I CC'TnA_GC
Cuts at: 398 1412 5969 398
Size: 1014 4557 4595
Fragments arranged by size:
4595 4557 1014
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1993 2997 4292 7342 8221 8713 9011 1993
Size: 1004 1295 3050 879 492 298 3148
Fragments arranged by size:
3148 3050 1295 1004 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 890 4709 5812 6779 6816 9870 890
Size: 3819 1103 967 37 3054 1186
Fragments arranged by size:
3819 3054 1186 1103 967 37
BsaAI yAC'GTr
Cuts at: 7519 7519
Size: 10166
BsaBI GATnn'nnATC
Cuts at: 6 1208 5216 6968 7680 9717 6
Size: 1202 4008 1752 712 2037 455
Fragments arranged by size:
4008 2037 1752 1202 712 455
BsaHI Gr'CG_yC
Cuts at: 1015 7597 7845 8547 10043 1015
Size: 6582 248 702 1496 1138
Fragments arranged by size:
6582 1496 1138 702 248
BsaWI w'CCGG_w
Cuts at: 6331 7355 7876 8554 8828 8975 9386 6331
Size: 1024 521 678 274 147 411 7111
Fragments arranged by size:
7111 1024 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1931 1961 2180 2210 4251 4281 6759 6782 6789
Size: 30 219 30 2041 30 2478 23 7
Cuts at: 6789 6812 1931
Size: 23 5285
Fragments arranged by size:
5285 2478 2041 219 30 30 30 23
23 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1348 1807 2511 4305 6085 6822 1348
Size: 459 704 1794 1780 737 4692
Fragments arranged by size:
4692 1794 1780 737 704 459
BseYI C'CCAG_C
Cuts at: 6187 7591 8349 8926 9422 6187
Size: 1404 758 577 496 6931
Fragments arranged by size:
6931 1404 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 1987 209
Size: 1778 8388
BsiEI CG_ry'CG
Cuts at: 104 7708 7754 8962 104
Size: 7604 46 1208 1308
Fragments arranged by size:
7604 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 122 965 2097 3099 3859 4216 5074 5708 6199
Size: 843 1132 1002 760 357 858 634 491
Cuts at: 6199 6355 7109 7180 7958 8148 8940 122
Size: 156 754 71 778 190 792 1348
Fragments arranged by size:
1348 1132 1002 858 843 792 778 760
754 634 491 357 190 156 71
BsmI GAATG_Cn'
Cuts at: 3317 6166 6635 3317
Size: 2849 469 6848
Fragments arranged by size:
6848 2849 469
Bsp1286I G_dGCh'C
Cuts at: 122 690 949 965 2097 3099 3415 3859 4216
Size: 568 259 16 1132 1002 316 444 357
Cuts at: 4216 5074 5227 5331 5708 5868 6199 6306 6355
Size: 858 153 104 377 160 331 107 49
Cuts at: 6355 6365 6432 7109 7180 7791 7884 7958 8148
Size: 10 67 677 71 611 93 74 190
Cuts at: 8148 8210 8940 122
Size: 62 730 1348
Fragments arranged by size:
1348 1132 1002 858 730 677 611 568
444 377 357 331 316 259 190 160
153 107 104 93 74 71 67 62
49 16 10
BspEI T'CCGG_A
Cuts at: 6331 9386 6331
Size: 3055 7111
BspHI T'CATG_A
Cuts at: 3303 3833 9342 3303
Size: 530 5509 4127
Fragments arranged by size:
5509 4127 530
BspMI ACCTGCnnnn'nnnn_
Cuts at: 136 570 2842 3114 7216 7430 7732 8113 136
Size: 434 2272 272 4102 214 302 381 2189
Fragments arranged by size:
4102 2272 2189 434 381 302 272 214
BsrBI CCG'CTC
Cuts at: 50 8458 8512 9996 10161 50
Size: 8408 54 1484 165 55
Fragments arranged by size:
8408 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1319 4331 4704 6018 6555 8078 1319
Size: 3012 373 1314 537 1523 3407
Fragments arranged by size:
3407 3012 1523 1314 537 373
BsrFI r'CCGG_y
Cuts at: 8164 8345 8554 8164
Size: 181 209 9776
Fragments arranged by size:
9776 209 181
BsrGI T'GTAC_A
Cuts at: 299 6089 6212 299
Size: 5790 123 4253
Fragments arranged by size:
5790 4253 123
BssSI C'ACGA_G
Cuts at: 1478 4723 8437 8795 1478
Size: 3245 3714 358 2849
Fragments arranged by size:
3714 3245 2849 358
BstAPI GCAn_nnn'nTGC
Cuts at: 7543 7543
Size: 10166
BstBI TT'CG_AA
Cuts at: 7083 8527 7083
Size: 1444 8722
BstXI CCAn_nnnn'nTGG
Cuts at: 1117 2225 2695 3248 4848 6848 1117
Size: 1108 470 553 1600 2000 4435
Fragments arranged by size:
4435 2000 1600 1108 553 470
BstYI r'GATC_y
Cuts at: 1 1737 1788 1977 4318 4719 4851 5889 6384
Size: 1736 51 189 2341 401 132 1038 495
Cuts at: 6384 7191 7658 7681 8016 8262 9274 9360 9372
Size: 807 467 23 335 246 1012 86 12
Cuts at: 9372 9718 1
Size: 346 449
Fragments arranged by size:
2341 1736 1038 1012 807 495 467 449
401 346 335 246 189 132 86 51
23 12
Bsu36I CC'TnA_GG
Cuts at: 2874 3558 6827 7120 2874
Size: 684 3269 293 5920
Fragments arranged by size:
5920 3269 684 293
BtgI C'CryG_G
Cuts at: 5409 5409
Size: 10166
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 330 991 8116 8297 8460 330
Size: 661 7125 181 163 2036
Fragments arranged by size:
7125 2036 661 181 163
BtsI GCAGTG_nn'
Cuts at: 197 5276 5346 7409 197
Size: 5079 70 2063 2954
Fragments arranged by size:
5079 2954 2063 70
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2910 2945 5072 5107 2910
Size: 35 2127 35 7969
Fragments arranged by size:
7969 2127 35 35
DraI TTT'AAA
Cuts at: 3084 3282 5901 6677 7165 3084
Size: 198 2619 776 488 6085
Fragments arranged by size:
6085 2619 776 488 198
DrdI GACnn_nn'nnGTC
Cuts at: 7872 8730 7872
Size: 858 9308
EaeI y'GGCC_r
Cuts at: 716 4710 6314 6966 7751 7925 8316 8343 9688
Size: 3994 1604 652 785 174 391 27 1345
Cuts at: 9688 716
Size: 1194
Fragments arranged by size:
3994 1604 1345 1194 785 652 391 174
27
EagI C'GGCC_G
Cuts at: 7751 7751
Size: 10166
EarI CTCTTCn'nnn_
Cuts at: 1107 1325 4407 4892 5132 6653 8189 8399 1107
Size: 218 3082 485 240 1521 1536 210 2874
Fragments arranged by size:
3082 2874 1536 1521 485 240 218 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 8374 8684 8830 9976 8374
Size: 310 146 1146 8564
Fragments arranged by size:
8564 1146 310 146
EcoICRI GAG'CTC
Cuts at: 120 3097 7178 120
Size: 2977 4081 3108
Fragments arranged by size:
4081 3108 2977
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 977 1349 1781 1982 2302 2651 2658 3884 4726
Size: 372 432 201 320 349 7 1226 842
Cuts at: 4726 5113 6266 6369 6429 6840 6936 7991 8423
Size: 387 1153 103 60 411 96 1055 432
Cuts at: 8423 9170 9438 977
Size: 747 268 1705
Fragments arranged by size:
1705 1226 1153 1055 842 747 432 432
411 387 372 349 320 268 201 103
96 60 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2720 4741 2720
Size: 2021 8145
EcoO109I rG'GnC_Cy
Cuts at: 382 582 1513 2878 3411 3412 3532 4383 4674
Size: 200 931 1365 533 1 120 851 291
Cuts at: 4674 6303 7152 7305 382
Size: 1629 849 153 3243
Fragments arranged by size:
3243 1629 1365 931 851 849 533 291
200 153 120 1
EcoRI G'AATT_C
Cuts at: 322 669 2397 7085 7170 322
Size: 347 1728 4688 85 3318
Fragments arranged by size:
4688 3318 1728 347 85
EcoRV GAT'ATC
Cuts at: 4055 4055
Size: 10166
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 825 4753 6381 6808 8185 825
Size: 3928 1628 427 1377 2806
Fragments arranged by size:
3928 2806 1628 1377 427
FspI TGC'GCA
Cuts at: 7947 7947
Size: 10166
HaeII r_GCGC'y
Cuts at: 7600 7848 8870 9849 7600
Size: 248 1022 979 7917
Fragments arranged by size:
7917 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 817 849 990 1022 1240 1272 2180 2212 2608
Size: 32 141 32 218 32 908 32 396
Cuts at: 2608 2640 3202 3234 3333 3365 3904 3936 4156
Size: 32 562 32 99 32 539 32 220
Cuts at: 4156 4188 4512 4544 4891 4923 5152 5184 5692
Size: 32 324 32 347 32 229 32 508
Cuts at: 5692 5724 5874 5906 6782 6814 8008 8038 8040
Size: 32 150 32 876 32 1194 30 2
Cuts at: 8040 8070 817
Size: 30 2913
Fragments arranged by size:
2913 1194 908 876 562 539 508 396
347 324 229 220 218 150 141 99
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3062 3116 5688 7205 3062
Size: 54 2572 1517 6023
Fragments arranged by size:
6023 2572 1517 54
HindIII A'AGCT_T
Cuts at: 485 1127 2385 485
Size: 642 1258 8266
Fragments arranged by size:
8266 1258 642
KasI G'GCGC_C
Cuts at: 7596 7844 7596
Size: 248 9918
KpnI G_GTAC'C
Cuts at: 1923 7186 1923
Size: 5263 4903
MluI A'CGCG_T
Cuts at: 9599 9599
Size: 10166
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1515 2021 3420 8837 9021 1515
Size: 506 1399 5417 184 2660
Fragments arranged by size:
5417 2660 1399 506 184
MscI TGG'CCA
Cuts at: 718 4712 6316 6968 7927 718
Size: 3994 1604 652 959 2957
Fragments arranged by size:
3994 2957 1604 959 652
MslI CAynn'nnrTG
Cuts at: 569 1112 2206 2693 3209 3388 6038 6182 6931
Size: 543 1094 487 516 179 2650 144 749
Cuts at: 6931 7039 8155 8282 569
Size: 108 1116 127 2453
Fragments arranged by size:
2650 2453 1116 1094 749 543 516 487
179 144 127 108
MspA1I CmG'CkG
Cuts at: 1148 1416 1960 7277 7591 7951 8964 9209 1148
Size: 268 544 5317 314 360 1013 245 2105
Fragments arranged by size:
5317 2105 1013 544 360 314 268 245
NaeI GCC'GGC
Cuts at: 8347 8347
Size: 10166
NarI GG'CG_CC
Cuts at: 7597 7845 7597
Size: 248 9918
NdeI CA'TA_TG
Cuts at: 570 3353 3882 3894 5630 570
Size: 2783 529 12 1736 5106
Fragments arranged by size:
5106 2783 1736 529 12
NgoMIV G'CCGG_C
Cuts at: 8345 8345
Size: 10166
NsiI A_TGCA'T
Cuts at: 4041 4041
Size: 10166
NspI r_CATG'y
Cuts at: 934 4043 5314 6049 6187 6693 6930 7219 7719
Size: 3109 1271 735 138 506 237 289 500
Cuts at: 7719 8626 934
Size: 907 2474
Fragments arranged by size:
3109 2474 1271 907 735 506 500 289
237 138
PasI CC'CwG_GG
Cuts at: 4548 6237 6423 4548
Size: 1689 186 8291
Fragments arranged by size:
8291 1689 186
PciI A'CATG_T
Cuts at: 5310 6045 8622 5310
Size: 735 2577 6854
Fragments arranged by size:
6854 2577 735
PflMI CCAn_nnn'nTGG
Cuts at: 2629 5828 2629
Size: 3199 6967
PfoI T'CCnGG_A
Cuts at: 5369 5369
Size: 10166
PmeI GTTT'AAAC
Cuts at: 7165 7165
Size: 10166
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9305 9337 9305
Size: 32 10134
PpuMI rG'GwC_Cy
Cuts at: 382 582 1513 2878 4383 4674 7152 382
Size: 200 931 1365 1505 291 2478 3396
Fragments arranged by size:
3396 2478 1505 1365 931 291 200
PshAI GACnn'nnGTC
Cuts at: 1148 4381 1148
Size: 3233 6933
PsiI TTA'TAA
Cuts at: 1385 1385
Size: 10166
PspOMI G'GGCC_C
Cuts at: 3411 6302 3411
Size: 2891 7275
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1151 1183 1151
Size: 32 10134
PstI C_TGCA'G
Cuts at: 2766 5271 5863 7213 7898 2766
Size: 2505 592 1350 685 5034
Fragments arranged by size:
5034 2505 1350 685 592
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10166
PvuII CAG'CTG
Cuts at: 1148 1960 7591 7951 1148
Size: 812 5631 360 3363
Fragments arranged by size:
5631 3363 812 360
RsrII CG'GwC_CG
Cuts at: 8361 8361
Size: 10166
SacI G_AGCT'C
Cuts at: 122 3099 7180 122
Size: 2977 4081 3108
Fragments arranged by size:
4081 3108 2977
SalI G'TCGA_C
Cuts at: 7203 7203
Size: 10166
SapI GCTCTTCn'nnn_
Cuts at: 8189 8399 8189
Size: 210 9956
SbfI CC_TGCA'GG
Cuts at: 7213 7213
Size: 10166
ScaI AGT'ACT
Cuts at: 3905 9683 3905
Size: 5778 4388
SexAI A'CCwGG_T
Cuts at: 653 6769 653
Size: 6116 4050
SfcI C'TryA_G
Cuts at: 33 1712 1838 2762 3332 5267 5859 7209 7894
Size: 1679 126 924 570 1935 592 1350 685
Cuts at: 7894 8887 9078 33
Size: 993 191 1121
Fragments arranged by size:
1935 1679 1350 1121 993 924 685 592
570 191 126
SfoI GGC'GCC
Cuts at: 7598 7846 7598
Size: 248 9918
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10166
SmaI CCC'GGG
Cuts at: 7188 7188
Size: 10166
SmlI C'TyrA_G
Cuts at: 427 1972 2976 4307 7321 8236 8728 8990 427
Size: 1545 1004 1331 3014 915 492 262 1603
Fragments arranged by size:
3014 1603 1545 1331 1004 915 492 262
SpeI A'CTAG_T
Cuts at: 67 693 67
Size: 626 9540
SphI G_CATG'C
Cuts at: 4043 6693 7219 7719 4043
Size: 2650 526 500 6490
Fragments arranged by size:
6490 2650 526 500
SspI AAT'ATT
Cuts at: 971 1396 1688 6579 971
Size: 425 292 4891 4558
Fragments arranged by size:
4891 4558 425 292
StuI AGG'CCT
Cuts at: 677 2726 5500 5714 677
Size: 2049 2774 214 5129
Fragments arranged by size:
5129 2774 2049 214
StyI C'CwwG_G
Cuts at: 355 719 1019 1071 2476 4511 6356 6801 7300
Size: 364 300 52 1405 2035 1845 445 499
Cuts at: 7300 7654 355
Size: 354 2867
Fragments arranged by size:
2867 2035 1845 1405 499 445 364 354
300 52
SwaI ATTT'AAAT
Cuts at: 6677 6677
Size: 10166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 175 5652 6635 7092 7749 7882 8341 8550 175
Size: 5477 983 457 657 133 459 209 1791
Fragments arranged by size:
5477 1791 983 657 459 457 209 133
TatI w'GTAC_w
Cuts at: 299 1430 2803 3903 5781 6089 6212 9681 299
Size: 1131 1373 1100 1878 308 123 3469 784
Fragments arranged by size:
3469 1878 1373 1131 1100 784 308 123
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2430 2462 6759 6791 9683 9715 2430
Size: 32 4297 32 2892 32 2881
Fragments arranged by size:
4297 2892 2881 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2552 7963 2552
Size: 5411 4755
XbaI T'CTAG_A
Cuts at: 7053 7197 7053
Size: 144 10022
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 362 1114 4509 4898 5926 7661 362
Size: 752 3395 389 1028 1735 2867
Fragments arranged by size:
3395 2867 1735 1028 752 389
XmaI C'CCGG_G
Cuts at: 7186 7186
Size: 10166
XmnI GAAnn'nnTTC
Cuts at: 1487 3844 4114 4646 6246 6940 1487
Size: 2357 270 532 1600 694 4713
Fragments arranged by size:
4713 2357 1600 694 532 270
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AscI AvrII BmgBI BmtI BsiWI
BsmBI BssHII BstEII BstZ17I ClaI DraIII FseI FspAI
HpaI MfeI NcoI NheI NotI NruI PacI PmlI
PspXI SacII SanDI SfiI SgrAI SnaBI SrfI XhoI
ZraI