Restriction map for
pF1KE2427
(Circular) (Six-base) MAPSORT of: pF1KE2427.seq Check: 1132 from: 1 to: 5006
pF1KE2427 5006 bp
With 182 enzymes: SgfI *
November 16, 2012 10:46 ..
AccI GT'mk_AC
Cuts at: 1883 2044 1883
Size: 161 4845
Acc65I G'GTAC_C
Cuts at: 2022 2022
Size: 5006
AclI AA'CG_TT
Cuts at: 4846 4846
Size: 5006
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 813 849 1241 2831 3263 4010 4278 813
Size: 36 392 1590 432 747 268 1541
Fragments arranged by size:
1590 1541 747 432 392 268 36
AflIII A'CryG_T
Cuts at: 1478 1901 1998 2358 3462 4439 1478
Size: 423 97 360 1104 977 2045
Fragments arranged by size:
2045 1104 977 423 360 97
AgeI A'CCGG_T
Cuts at: 1175 3394 1175
Size: 2219 2787
AleI CACnn'nnGTG
Cuts at: 1726 1726
Size: 5006
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1981 1981
Size: 5006
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 832 864 832
Size: 32 4974
AlwNI CAG_nnn'CTG
Cuts at: 167 600 886 1005 1692 2311 3878 167
Size: 433 286 119 687 619 1567 1295
Fragments arranged by size:
1567 1295 687 619 433 286 119
ApaLI G'TGCA_C
Cuts at: 3776 3776
Size: 5006
ApoI r'AATT_y
Cuts at: 246 1110 1126 1519 1682 2010 4359 4615 246
Size: 864 16 393 163 328 2349 256 637
Fragments arranged by size:
2349 864 637 393 328 256 163 16
AseI AT'TA_AT
Cuts at: 20 20
Size: 5006
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5006
AvaI C'yCGr_G
Cuts at: 688 1289 2026 688
Size: 601 737 3668
Fragments arranged by size:
3668 737 601
BamHI G'GATC_C
Cuts at: 1787 2031 1787
Size: 244 4762
BanI G'GyrC_C
Cuts at: 111 300 1537 1798 2022 2436 2684 2719 111
Size: 189 1237 261 224 414 248 35 2398
Fragments arranged by size:
2398 1237 414 261 248 224 189 35
BanII G_rGCy'C
Cuts at: 174 940 1202 2020 3050 174
Size: 766 262 818 1030 2130
Fragments arranged by size:
2130 1030 818 766 262
BbeI G_GCGC'C
Cuts at: 115 2440 2688 115
Size: 2325 248 2433
Fragments arranged by size:
2433 2325 248
BbsI GAAGACnn'nnnn_
Cuts at: 126 1078 1438 126
Size: 952 360 3694
Fragments arranged by size:
3694 952 360
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1308 1342 1308
Size: 34 4972
BciVI GTATCCnnnnn_n'
Cuts at: 1491 2904 3665 5006 1491
Size: 1413 761 1341 1491
Fragments arranged by size:
1491 1413 1341 761
BclI T'GATC_A
Cuts at: 2526 2526
Size: 5006
BglI GCCn_nnn'nGGC
Cuts at: 391 1978 391
Size: 1587 3419
BglII A'GATC_T
Cuts at: 334 410 435 479 1211 2521 4558 334
Size: 76 25 44 732 1310 2037 782
Fragments arranged by size:
2037 1310 782 732 76 44 25
BlpI GC'TnA_GC
Cuts at: 2118 2118
Size: 5006
Bme1580I G_kGCm'C
Cuts at: 549 1321 2631 2724 3780 549
Size: 772 1310 93 1056 1775
Fragments arranged by size:
1775 1310 1056 772 93
BmgBI CAC'GTC
Cuts at: 207 207
Size: 5006
BmrI ACTGGGnnnn_n'
Cuts at: 2218 2299 2634 4778 2218
Size: 81 335 2144 2446
Fragments arranged by size:
2446 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 870 3373 870
Size: 2503 2503
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 1051 688
Size: 363 4643
Bpu10I CC'TnA_GC
Cuts at: 296 296
Size: 5006
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2182 3061 3553 3851 2182
Size: 879 492 298 3337
Fragments arranged by size:
3337 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 421 4710 421
Size: 4289 717
BsaAI yAC'GTr
Cuts at: 912 2359 912
Size: 1447 3559
BsaBI GATnn'nnATC
Cuts at: 6 1262 2520 4557 6
Size: 1256 1258 2037 455
Fragments arranged by size:
2037 1258 1256 455
BsaHI Gr'CG_yC
Cuts at: 112 178 2437 2685 3387 4883 112
Size: 66 2259 248 702 1496 235
Fragments arranged by size:
2259 1496 702 248 235 66
BsaWI w'CCGG_w
Cuts at: 1175 1790 2195 2716 3394 3668 3815 4226 1175
Size: 615 405 521 678 274 147 411 1955
Fragments arranged by size:
1955 678 615 521 411 405 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1809 1839 1809
Size: 30 4976
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 586 654 667 1388 586
Size: 68 13 721 4204
Fragments arranged by size:
4204 721 68 13
BseYI C'CCAG_C
Cuts at: 349 623 1302 1866 1973 2431 3189 3766 4262
Size: 274 679 564 107 458 758 577 496
Cuts at: 4262 349
Size: 1093
Fragments arranged by size:
1093 758 679 577 564 496 458 274
107
BsiEI CG_ry'CG
Cuts at: 104 2548 2594 3802 104
Size: 2444 46 1208 1308
Fragments arranged by size:
2444 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 297 893 1202 2020 2798 2988 3780 297
Size: 596 309 818 778 190 792 1523
Fragments arranged by size:
1523 818 792 778 596 309 190
BsmBI CGTCTCn'nnnn_
Cuts at: 565 565
Size: 5006
Bsp1286I G_dGCh'C
Cuts at: 174 297 549 893 940 1202 1321 2020 2631
Size: 123 252 344 47 262 119 699 611
Cuts at: 2631 2724 2798 2988 3050 3780 174
Size: 93 74 190 62 730 1400
Fragments arranged by size:
1400 730 699 611 344 262 252 190
123 119 93 74 62 47
BspEI T'CCGG_A
Cuts at: 1790 4226 1790
Size: 2436 2570
BspHI T'CATG_A
Cuts at: 954 1115 4182 954
Size: 161 3067 1778
Fragments arranged by size:
3067 1778 161
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2056 2270 2572 2953 2056
Size: 214 302 381 4109
Fragments arranged by size:
4109 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3298 3352 4836 5001 50
Size: 3248 54 1484 165 55
Fragments arranged by size:
3248 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 360 1271 2918 360
Size: 911 1647 2448
Fragments arranged by size:
2448 1647 911
BsrFI r'CCGG_y
Cuts at: 1175 3004 3185 3394 1175
Size: 1829 181 209 2787
Fragments arranged by size:
2787 1829 209 181
BsrGI T'GTAC_A
Cuts at: 1481 1481
Size: 5006
BssSI C'ACGA_G
Cuts at: 3277 3635 3277
Size: 358 4648
BstAPI GCAn_nnn'nTGC
Cuts at: 544 2383 544
Size: 1839 3167
BstBI TT'CG_AA
Cuts at: 3367 3367
Size: 5006
BstXI CCAn_nnnn'nTGG
Cuts at: 357 1435 1592 357
Size: 1078 157 3771
Fragments arranged by size:
3771 1078 157
BstYI r'GATC_y
Cuts at: 1 334 410 435 479 1211 1787 2031 2498
Size: 333 76 25 44 732 576 244 467
Cuts at: 2498 2521 2856 3102 4114 4200 4212 4558 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 732 576 467 449 346 335 333
246 244 86 76 44 25 23 12
Bsu36I CC'TnA_GG
Cuts at: 637 932 1625 637
Size: 295 693 4018
Fragments arranged by size:
4018 693 295
BtgI C'CryG_G
Cuts at: 107 1253 1724 107
Size: 1146 471 3389
Fragments arranged by size:
3389 1146 471
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1311 2956 3137 3300 1311
Size: 1645 181 163 3017
Fragments arranged by size:
3017 1645 181 163
BtsI GCAGTG_nn'
Cuts at: 1742 1944 2249 1742
Size: 202 305 4499
Fragments arranged by size:
4499 305 202
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1206 1241 1206
Size: 35 4971
DraI TTT'AAA
Cuts at: 2005 2005
Size: 5006
DraIII CAC_nnn'GTG
Cuts at: 544 544
Size: 5006
DrdI GACnn_nn'nnGTC
Cuts at: 1153 1650 2712 3570 1153
Size: 497 1062 858 2589
Fragments arranged by size:
2589 1062 858 497
EaeI y'GGCC_r
Cuts at: 1437 1925 2591 2765 3156 3183 4528 1437
Size: 488 666 174 391 27 1345 1915
Fragments arranged by size:
1915 1345 666 488 391 174 27
EagI C'GGCC_G
Cuts at: 2591 2591
Size: 5006
EarI CTCTTCn'nnn_
Cuts at: 373 1705 1780 3029 3239 373
Size: 1332 75 1249 210 2140
Fragments arranged by size:
2140 1332 1249 210 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3214 3524 3670 4816 3214
Size: 310 146 1146 3404
Fragments arranged by size:
3404 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1200 2018 1200
Size: 818 4188
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 813 849 1051 1241 2831 3263 4010 4278
Size: 125 36 202 190 1590 432 747 268
Cuts at: 4278 688
Size: 1416
Fragments arranged by size:
1590 1416 747 432 268 202 190 125
36
EcoO109I rG'GnC_Cy
Cuts at: 393 663 1715 2145 393
Size: 270 1052 430 3254
Fragments arranged by size:
3254 1052 430 270
EcoRI G'AATT_C
Cuts at: 2010 2010
Size: 5006
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 457 858 1095 3025 457
Size: 401 237 1930 2438
Fragments arranged by size:
2438 1930 401 237
FspI TGC'GCA
Cuts at: 2787 2787
Size: 5006
HaeII r_GCGC'y
Cuts at: 115 2440 2688 3710 4689 115
Size: 2325 248 1022 979 432
Fragments arranged by size:
2325 1022 979 432 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 168 200 395 420 427 451 452 483 1046
Size: 32 195 25 7 24 1 31 563
Cuts at: 1046 1078 1196 1228 1516 1548 2848 2878 2880
Size: 32 118 32 288 32 1300 30 2
Cuts at: 2880 2910 168
Size: 30 2264
Fragments arranged by size:
2264 1300 563 288 195 118 32 32
32 32 31 30 30 25 24 7
2 1
HincII GTy'rAC
Cuts at: 2045 2045
Size: 5006
KasI G'GCGC_C
Cuts at: 111 2436 2684 111
Size: 2325 248 2433
Fragments arranged by size:
2433 2325 248
KpnI G_GTAC'C
Cuts at: 2026 2026
Size: 5006
MluI A'CGCG_T
Cuts at: 4439 4439
Size: 5006
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 971 1440 1835 3677 3861 971
Size: 469 395 1842 184 2116
Fragments arranged by size:
2116 1842 469 395 184
MscI TGG'CCA
Cuts at: 1439 1927 2767 1439
Size: 488 840 3678
Fragments arranged by size:
3678 840 488
MslI CAynn'nnrTG
Cuts at: 1726 2995 3122 1726
Size: 1269 127 3610
Fragments arranged by size:
3610 1269 127
MspA1I CmG'CkG
Cuts at: 451 813 883 1365 2117 2431 2791 3804 4049
Size: 362 70 482 752 314 360 1013 245
Cuts at: 4049 451
Size: 1408
Fragments arranged by size:
1408 1013 752 482 362 360 314 245
70
NaeI GCC'GGC
Cuts at: 3187 3187
Size: 5006
NarI GG'CG_CC
Cuts at: 112 2437 2685 112
Size: 2325 248 2433
Fragments arranged by size:
2433 2325 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5006
NgoMIV G'CCGG_C
Cuts at: 3185 3185
Size: 5006
NspI r_CATG'y
Cuts at: 323 1036 1858 1905 2002 2059 2559 3466 323
Size: 713 822 47 97 57 500 907 1863
Fragments arranged by size:
1863 907 822 713 500 97 57 47
PciI A'CATG_T
Cuts at: 1901 1998 3462 1901
Size: 97 1464 3445
Fragments arranged by size:
3445 1464 97
PflMI CCAn_nnn'nTGG
Cuts at: 167 1446 167
Size: 1279 3727
PmeI GTTT'AAAC
Cuts at: 2005 2005
Size: 5006
PmlI CAC'GTG
Cuts at: 912 912
Size: 5006
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4145 4177 4145
Size: 32 4974
PpuMI rG'GwC_Cy
Cuts at: 663 663
Size: 5006
PshAI GACnn'nnGTC
Cuts at: 1411 1411
Size: 5006
PstI C_TGCA'G
Cuts at: 605 882 2053 2738 605
Size: 277 1171 685 2873
Fragments arranged by size:
2873 1171 685 277
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5006
PvuII CAG'CTG
Cuts at: 451 813 883 1365 2431 2791 451
Size: 362 70 482 1066 360 2666
Fragments arranged by size:
2666 1066 482 362 360 70
RsrII CG'GwC_CG
Cuts at: 3201 3201
Size: 5006
SacI G_AGCT'C
Cuts at: 1202 2020 1202
Size: 818 4188
SalI G'TCGA_C
Cuts at: 2043 2043
Size: 5006
SapI GCTCTTCn'nnn_
Cuts at: 1780 3029 3239 1780
Size: 1249 210 3547
Fragments arranged by size:
3547 1249 210
SbfI CC_TGCA'GG
Cuts at: 2053 2053
Size: 5006
ScaI AGT'ACT
Cuts at: 4523 4523
Size: 5006
SfcI C'TryA_G
Cuts at: 33 601 878 1088 2049 2734 3727 3918 33
Size: 568 277 210 961 685 993 191 1121
Fragments arranged by size:
1121 993 961 685 568 277 210 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 391 391
Size: 5006
SfoI GGC'GCC
Cuts at: 113 2438 2686 113
Size: 2325 248 2433
Fragments arranged by size:
2433 2325 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5006
SmaI CCC'GGG
Cuts at: 1291 2028 1291
Size: 737 4269
SmlI C'TyrA_G
Cuts at: 2161 3076 3568 3830 2161
Size: 915 492 262 3337
Fragments arranged by size:
3337 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5006
SphI G_CATG'C
Cuts at: 2059 2559 2059
Size: 500 4506
StyI C'CwwG_G
Cuts at: 107 288 521 1313 2140 2494 107
Size: 181 233 792 827 354 2619
Fragments arranged by size:
2619 827 792 354 233 181
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1293 2589 2722 3181 3390 1293
Size: 1296 133 459 209 2909
Fragments arranged by size:
2909 1296 459 209 133
TatI w'GTAC_w
Cuts at: 1481 4521 1481
Size: 3040 1966
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1809 1841 4523 4555 1809
Size: 32 2682 32 2260
Fragments arranged by size:
2682 2260 32 32
Tth111I GACn'n_nGTC
Cuts at: 2803 2803
Size: 5006
XbaI T'CTAG_A
Cuts at: 2037 2037
Size: 5006
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 204 2501 204
Size: 2297 2709
XmaI C'CCGG_G
Cuts at: 1289 2026 1289
Size: 737 4269
XmnI GAAnn'nnTTC
Cuts at: 1663 1663
Size: 5006
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI ApaI AscI AvrII
BaeI BbvCI BfrBI BmtI BsgI BsiWI BsmI BssHII
BstEII BstZ17I ClaI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI