Restriction map for
pF1KE2429
(Circular) (Six-base) MAPSORT of: pF1KE2429.seq Check: 6573 from: 1 to: 5087
pF1KE2429 5087 bp
With 182 enzymes: SgfI *
December 4, 2012 11:59 ..
AccI GT'mk_AC
Cuts at: 2125 2125
Size: 5087
Acc65I G'GTAC_C
Cuts at: 2103 2103
Size: 5087
AclI AA'CG_TT
Cuts at: 4927 4927
Size: 5087
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 946 1495 1675 2912 3344 4091 4359 616
Size: 330 549 180 1237 432 747 268 1344
Fragments arranged by size:
1344 1237 747 549 432 330 268 180
AflIII A'CryG_T
Cuts at: 2045 2439 3543 4520 2045
Size: 394 1104 977 2612
Fragments arranged by size:
2612 1104 977 394
AgeI A'CCGG_T
Cuts at: 3475 3475
Size: 5087
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 241 422 241
Size: 181 4906
AlwNI CAG_nnn'CTG
Cuts at: 210 1023 2392 3959 210
Size: 813 1369 1567 1338
Fragments arranged by size:
1567 1369 1338 813
ApaI G_GGCC'C
Cuts at: 148 200 148
Size: 52 5035
ApaLI G'TGCA_C
Cuts at: 305 3857 305
Size: 3552 1535
ApoI r'AATT_y
Cuts at: 855 1468 2091 4440 4696 855
Size: 613 623 2349 256 1246
Fragments arranged by size:
2349 1246 623 613 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5087
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5087
AvaI C'yCGr_G
Cuts at: 140 476 557 958 2006 2107 140
Size: 336 81 401 1048 101 3120
Fragments arranged by size:
3120 1048 401 336 101 81
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 288 321 288
Size: 33 5054
BamHI G'GATC_C
Cuts at: 2112 2112
Size: 5087
BanI G'GyrC_C
Cuts at: 111 2103 2517 2765 2800 111
Size: 1992 414 248 35 2398
Fragments arranged by size:
2398 1992 414 248 35
BanII G_rGCy'C
Cuts at: 148 168 200 904 1013 1495 1667 1826 2101
Size: 20 32 704 109 482 172 159 275
Cuts at: 2101 3131 148
Size: 1030 2104
Fragments arranged by size:
2104 1030 704 482 275 172 159 109
32 20
BbeI G_GCGC'C
Cuts at: 2521 2769 2521
Size: 248 4839
BbsI GAAGACnn'nnnn_
Cuts at: 287 604 2048 287
Size: 317 1444 3326
Fragments arranged by size:
3326 1444 317
BbvCI CC'TCA_GC
Cuts at: 181 181
Size: 5087
BciVI GTATCCnnnnn_n'
Cuts at: 746 2985 3746 5087 746
Size: 2239 761 1341 746
Fragments arranged by size:
2239 1341 761 746
BclI T'GATC_A
Cuts at: 2607 2607
Size: 5087
BglI GCCn_nnn'nGGC
Cuts at: 1513 1671 1513
Size: 158 4929
BglII A'GATC_T
Cuts at: 2602 4639 2602
Size: 2037 3050
BlpI GC'TnA_GC
Cuts at: 1489 2199 1489
Size: 710 4377
Bme1580I G_kGCm'C
Cuts at: 116 148 200 230 309 2712 2805 3861 116
Size: 32 52 30 79 2403 93 1056 1342
Fragments arranged by size:
2403 1342 1056 93 79 52 32 30
BmrI ACTGGGnnnn_n'
Cuts at: 164 747 788 2299 2380 2715 4859 164
Size: 583 41 1511 81 335 2144 392
Fragments arranged by size:
2144 1511 583 392 335 81 41
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3454 3454
Size: 5087
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 1653 640
Size: 1013 4074
Bpu10I CC'TnA_GC
Cuts at: 181 1012 1397 1820 181
Size: 831 385 423 3448
Fragments arranged by size:
3448 831 423 385
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2263 3142 3634 3932 2263
Size: 879 492 298 3418
Fragments arranged by size:
3418 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1600 1929 4791 1600
Size: 329 2862 1896
Fragments arranged by size:
2862 1896 329
BsaAI yAC'GTr
Cuts at: 2440 2440
Size: 5087
BsaBI GATnn'nnATC
Cuts at: 6 2601 4638 6
Size: 2595 2037 455
Fragments arranged by size:
2595 2037 455
BsaHI Gr'CG_yC
Cuts at: 2518 2766 3468 4964 2518
Size: 248 702 1496 2641
Fragments arranged by size:
2641 1496 702 248
BsaWI w'CCGG_w
Cuts at: 587 2276 2797 3475 3749 3896 4307 587
Size: 1689 521 678 274 147 411 1367
Fragments arranged by size:
1689 1367 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1057 1472 1057
Size: 415 4672
BseYI C'CCAG_C
Cuts at: 1508 1890 2512 3270 3847 4343 1508
Size: 382 622 758 577 496 2252
Fragments arranged by size:
2252 758 622 577 496 382
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1363 1363
Size: 5087
BsiEI CG_ry'CG
Cuts at: 104 1599 2629 2675 3883 104
Size: 1495 1030 46 1208 1308
Fragments arranged by size:
1495 1308 1208 1030 46
BsiHKAI G_wGCw'C
Cuts at: 309 1013 1223 1250 1645 1714 1826 1879 2101
Size: 704 210 27 395 69 112 53 222
Cuts at: 2101 2879 3069 3861 309
Size: 778 190 792 1535
Fragments arranged by size:
1535 792 778 704 395 222 210 190
112 69 53 27
BsmI GAATG_Cn'
Cuts at: 1516 1585 1516
Size: 69 5018
Bsp1286I G_dGCh'C
Cuts at: 116 148 168 200 230 309 904 1013 1223
Size: 32 20 32 30 79 595 109 210
Cuts at: 1223 1250 1495 1645 1667 1714 1826 1879 2101
Size: 27 245 150 22 47 112 53 222
Cuts at: 2101 2712 2805 2879 3069 3131 3861 116
Size: 611 93 74 190 62 730 1342
Fragments arranged by size:
1342 730 611 595 245 222 210 190
150 112 109 93 79 74 62 53
47 32 32 30 27 22 20
BspEI T'CCGG_A
Cuts at: 4307 4307
Size: 5087
BspHI T'CATG_A
Cuts at: 1956 4263 1956
Size: 2307 2780
BspMI ACCTGCnnnn'nnnn_
Cuts at: 218 2137 2351 2653 3034 218
Size: 1919 214 302 381 2271
Fragments arranged by size:
2271 1919 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3379 3433 4917 5082 50
Size: 3329 54 1484 165 55
Fragments arranged by size:
3329 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1044 1754 1983 2007 2999 1044
Size: 710 229 24 992 3132
Fragments arranged by size:
3132 992 710 229 24
BsrFI r'CCGG_y
Cuts at: 187 3085 3266 3475 187
Size: 2898 181 209 1799
Fragments arranged by size:
2898 1799 209 181
BssHII G'CGCG_C
Cuts at: 129 129
Size: 5087
BssSI C'ACGA_G
Cuts at: 1872 3358 3716 1872
Size: 1486 358 3243
Fragments arranged by size:
3243 1486 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2464 2464
Size: 5087
BstBI TT'CG_AA
Cuts at: 3448 3448
Size: 5087
BstYI r'GATC_y
Cuts at: 1 2112 2579 2602 2937 3183 4195 4281 4293
Size: 2111 467 23 335 246 1012 86 12
Cuts at: 4293 4639 1
Size: 346 449
Fragments arranged by size:
2111 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 844 1051 844
Size: 207 4880
BtgI C'CryG_G
Cuts at: 107 149 1722 107
Size: 42 1573 3472
Fragments arranged by size:
3472 1573 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3037 3218 3381 3037
Size: 181 163 4743
Fragments arranged by size:
4743 181 163
BtsI GCAGTG_nn'
Cuts at: 1076 1250 1577 1901 2330 1076
Size: 174 327 324 429 3833
Fragments arranged by size:
3833 429 327 324 174
DraI TTT'AAA
Cuts at: 1272 2086 1272
Size: 814 4273
DrdI GACnn_nn'nnGTC
Cuts at: 1333 2793 3651 1333
Size: 1460 858 2769
Fragments arranged by size:
2769 1460 858
EaeI y'GGCC_r
Cuts at: 201 330 1556 1596 1725 2672 2846 3237 3264
Size: 129 1226 40 129 947 174 391 27
Cuts at: 3264 4609 201
Size: 1345 679
Fragments arranged by size:
1345 1226 947 679 391 174 129 129
40 27
EagI C'GGCC_G
Cuts at: 1596 2672 1596
Size: 1076 4011
EarI CTCTTCn'nnn_
Cuts at: 814 1989 3110 3320 814
Size: 1175 1121 210 2581
Fragments arranged by size:
2581 1175 1121 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1603 3295 3605 3751 4897 1603
Size: 1692 310 146 1146 1793
Fragments arranged by size:
1793 1692 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1011 1824 2099 1011
Size: 813 275 3999
Fragments arranged by size:
3999 813 275
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 640 946 1495 1653 1675 2912 3344 4091
Size: 24 306 549 158 22 1237 432 747
Cuts at: 4091 4359 616
Size: 268 1344
Fragments arranged by size:
1344 1237 747 549 432 306 268 158
24 22
EcoNI CCTnn'n_nnAGG
Cuts at: 1656 1656
Size: 5087
EcoO109I rG'GnC_Cy
Cuts at: 144 145 448 716 1107 2226 144
Size: 1 303 268 391 1119 3005
Fragments arranged by size:
3005 1119 391 303 268 1
EcoRI G'AATT_C
Cuts at: 1468 2091 1468
Size: 623 4464
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 336 3106 336
Size: 2770 2317
FspI TGC'GCA
Cuts at: 2868 2868
Size: 5087
HaeII r_GCGC'y
Cuts at: 188 2521 2769 3791 4770 188
Size: 2333 248 1022 979 505
Fragments arranged by size:
2333 1022 979 505 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 517 549 2929 2959 2961 2991 517
Size: 32 2380 30 2 30 2613
Fragments arranged by size:
2613 2380 32 30 30 2
HincII GTy'rAC
Cuts at: 2126 2126
Size: 5087
KasI G'GCGC_C
Cuts at: 2517 2765 2517
Size: 248 4839
KpnI G_GTAC'C
Cuts at: 2107 2107
Size: 5087
MluI A'CGCG_T
Cuts at: 4520 4520
Size: 5087
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3758 3942 3758
Size: 184 4903
MscI TGG'CCA
Cuts at: 332 1558 1727 2848 332
Size: 1226 169 1121 2571
Fragments arranged by size:
2571 1226 1121 169
MslI CAynn'nnrTG
Cuts at: 1142 3076 3203 1142
Size: 1934 127 3026
Fragments arranged by size:
3026 1934 127
MspA1I CmG'CkG
Cuts at: 151 402 1157 1393 1512 1731 2198 2512 2872
Size: 251 755 236 119 219 467 314 360
Cuts at: 2872 3885 4130 151
Size: 1013 245 1108
Fragments arranged by size:
1108 1013 755 467 360 314 251 245
236 219 119
NaeI GCC'GGC
Cuts at: 189 3268 189
Size: 3079 2008
NarI GG'CG_CC
Cuts at: 2518 2766 2518
Size: 248 4839
NcoI C'CATG_G
Cuts at: 107 1722 107
Size: 1615 3472
NgoMIV G'CCGG_C
Cuts at: 187 3266 187
Size: 3079 2008
NspI r_CATG'y
Cuts at: 2049 2140 2640 3547 2049
Size: 91 500 907 3589
Fragments arranged by size:
3589 907 500 91
PciI A'CATG_T
Cuts at: 2045 3543 2045
Size: 1498 3589
PflMI CCAn_nnn'nTGG
Cuts at: 2025 2025
Size: 5087
PfoI T'CCnGG_A
Cuts at: 159 875 159
Size: 716 4371
PmeI GTTT'AAAC
Cuts at: 2086 2086
Size: 5087
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4226 4258 4226
Size: 32 5055
PpuMI rG'GwC_Cy
Cuts at: 448 1107 448
Size: 659 4428
PspOMI G'GGCC_C
Cuts at: 144 196 144
Size: 52 5035
PstI C_TGCA'G
Cuts at: 215 2134 2819 215
Size: 1919 685 2483
Fragments arranged by size:
2483 1919 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5087
PvuII CAG'CTG
Cuts at: 402 1393 1512 1731 2512 2872 402
Size: 991 119 219 781 360 2617
Fragments arranged by size:
2617 991 781 360 219 119
RsrII CG'GwC_CG
Cuts at: 3282 3282
Size: 5087
SacI G_AGCT'C
Cuts at: 1013 1826 2101 1013
Size: 813 275 3999
Fragments arranged by size:
3999 813 275
SacII CC_GC'GG
Cuts at: 152 152
Size: 5087
SalI G'TCGA_C
Cuts at: 2124 2124
Size: 5087
SanDI GG'GwC_CC
Cuts at: 448 448
Size: 5087
SapI GCTCTTCn'nnn_
Cuts at: 3110 3320 3110
Size: 210 4877
SbfI CC_TGCA'GG
Cuts at: 2134 2134
Size: 5087
ScaI AGT'ACT
Cuts at: 4604 4604
Size: 5087
SexAI A'CCwGG_T
Cuts at: 722 722
Size: 5087
SfcI C'TryA_G
Cuts at: 33 211 1572 2130 2815 3808 3999 33
Size: 178 1361 558 685 993 191 1121
Fragments arranged by size:
1361 1121 993 685 558 191 178
SfoI GGC'GCC
Cuts at: 2519 2767 2519
Size: 248 4839
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5087
SmaI CCC'GGG
Cuts at: 142 559 2008 2109 142
Size: 417 1449 101 3120
Fragments arranged by size:
3120 1449 417 101
SmlI C'TyrA_G
Cuts at: 476 2242 3157 3649 3911 476
Size: 1766 915 492 262 1652
Fragments arranged by size:
1766 1652 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5087
SphI G_CATG'C
Cuts at: 2140 2640 2140
Size: 500 4587
StuI AGG'CCT
Cuts at: 1651 2035 1651
Size: 384 4703
StyI C'CwwG_G
Cuts at: 107 1190 1722 1902 2221 2575 107
Size: 1083 532 180 319 354 2619
Fragments arranged by size:
2619 1083 532 354 319 180
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1781 2670 2803 3262 3471 1781
Size: 889 133 459 209 3397
Fragments arranged by size:
3397 889 459 209 133
TatI w'GTAC_w
Cuts at: 929 4602 929
Size: 3673 1414
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4604 4636 4604
Size: 32 5055
Tth111I GACn'n_nGTC
Cuts at: 1111 1441 2884 1111
Size: 330 1443 3314
Fragments arranged by size:
3314 1443 330
XbaI T'CTAG_A
Cuts at: 2118 2118
Size: 5087
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 834 2582 834
Size: 1748 3339
XhoI C'TCGA_G
Cuts at: 476 476
Size: 5087
XmaI C'CCGG_G
Cuts at: 140 557 2006 2107 140
Size: 417 1449 101 3120
Fragments arranged by size:
3120 1449 417 101
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BcgI BfrBI BmgBI BmtI BsaXI BsiWI BsmBI
BsrGI BstEII BstXI BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PspXI
PsrI SfiI SgrAI SnaBI SrfI SspI SwaI XmnI
ZraI