Restriction map for
pF1KE2457
(Circular) (Six-base) MAPSORT of: pF1KE2457.seq Check: 6372 from: 1 to: 6641
pF1KE2457 6641 bp
With 182 enzymes: SgfI *
April 12, 2013 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2845 3364 3419 2845
Size: 519 55 6067
Fragments arranged by size:
6067 519 55
AccI GT'mk_AC
Cuts at: 3332 3679 3332
Size: 347 6294
Acc65I G'GTAC_C
Cuts at: 3657 3657
Size: 6641
AclI AA'CG_TT
Cuts at: 6481 6481
Size: 6641
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 1114 1543 1926 2871 3282 3307 4466 4898
Size: 885 429 383 945 411 25 1159 432
Cuts at: 4898 5645 5913 229
Size: 747 268 957
Fragments arranged by size:
1159 957 945 885 747 432 429 411
383 268 25
AfeI AGC'GCT
Cuts at: 895 895
Size: 6641
AflII C'TTAA_G
Cuts at: 2916 2916
Size: 6641
AflIII A'CryG_T
Cuts at: 3500 3993 5097 6074 3500
Size: 493 1104 977 4067
Fragments arranged by size:
4067 1104 977 493
AgeI A'CCGG_T
Cuts at: 5029 5029
Size: 6641
AleI CACnn'nnGTG
Cuts at: 3358 3358
Size: 6641
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 310 925 310
Size: 615 6026
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 370 402 1120 1152 370
Size: 32 718 32 5859
Fragments arranged by size:
5859 718 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1092 1332 2111 2814 2864 3156 3411 3946 5513
Size: 240 779 703 50 292 255 535 1567
Cuts at: 5513 1092
Size: 2220
Fragments arranged by size:
2220 1567 779 703 535 292 255 240
50
ApaI G_GGCC'C
Cuts at: 744 1274 1533 1645 3127 3425 3528 744
Size: 530 259 112 1482 298 103 3857
Fragments arranged by size:
3857 1482 530 298 259 112 103
ApaLI G'TGCA_C
Cuts at: 2832 5411 2832
Size: 2579 4062
ApoI r'AATT_y
Cuts at: 1005 3645 5994 6250 1005
Size: 2640 2349 256 1396
Fragments arranged by size:
2640 2349 1396 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6641
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6641
AvaI C'yCGr_G
Cuts at: 793 1075 1879 2649 3066 3119 3661 793
Size: 282 804 770 417 53 542 3773
Fragments arranged by size:
3773 804 770 542 417 282 53
BamHI G'GATC_C
Cuts at: 3666 3666
Size: 6641
BanI G'GyrC_C
Cuts at: 370 2593 3657 4071 4319 4354 370
Size: 2223 1064 414 248 35 2657
Fragments arranged by size:
2657 2223 1064 414 248 35
BanII G_rGCy'C
Cuts at: 744 1274 1533 1645 1907 1981 2381 2620 2740
Size: 530 259 112 262 74 400 239 120
Cuts at: 2740 3127 3212 3425 3528 3655 4685 744
Size: 387 85 213 103 127 1030 2700
Fragments arranged by size:
2700 1030 530 400 387 262 259 239
213 127 120 112 103 85 74
BbeI G_GCGC'C
Cuts at: 374 4075 4323 374
Size: 3701 248 2692
Fragments arranged by size:
3701 2692 248
BbsI GAAGACnn'nnnn_
Cuts at: 217 1739 3219 3254 217
Size: 1522 1480 35 3604
Fragments arranged by size:
3604 1522 1480 35
BbvCI CC'TCA_GC
Cuts at: 2333 3623 2333
Size: 1290 5351
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3047 3081 3477 3511 3047
Size: 34 396 34 6177
Fragments arranged by size:
6177 396 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4539 5300 6641 4539
Size: 761 1341 4539
Fragments arranged by size:
4539 1341 761
BclI T'GATC_A
Cuts at: 4161 4161
Size: 6641
BfrBI ATG'CAT
Cuts at: 2156 2156
Size: 6641
BglI GCCn_nnn'nGGC
Cuts at: 583 739 1110 1615 1787 2039 2962 2983 3122
Size: 156 371 505 172 252 923 21 139
Cuts at: 3122 3420 583
Size: 298 3804
Fragments arranged by size:
3804 923 505 371 298 252 172 156
139 21
BglII A'GATC_T
Cuts at: 4156 6193 4156
Size: 2037 4604
BlpI GC'TnA_GC
Cuts at: 1584 3753 1584
Size: 2169 4472
Bme1580I G_kGCm'C
Cuts at: 120 744 1274 1386 1533 1645 2596 2836 3127
Size: 624 530 112 147 112 951 240 291
Cuts at: 3127 3425 3528 4266 4359 5415 120
Size: 298 103 738 93 1056 1346
Fragments arranged by size:
1346 1056 951 738 624 530 298 291
240 147 112 112 103 93
BmgBI CAC'GTC
Cuts at: 885 3222 3252 885
Size: 2337 30 4274
Fragments arranged by size:
4274 2337 30
BmrI ACTGGGnnnn_n'
Cuts at: 777 2714 3004 3410 3853 3934 4269 6413 777
Size: 1937 290 406 443 81 335 2144 1005
Fragments arranged by size:
2144 1937 1005 443 406 335 290 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 367 1121 5008 367
Size: 754 3887 2000
Fragments arranged by size:
3887 2000 754
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 144 403 1354 1614 2440 2455 2464 2584 144
Size: 259 951 260 826 15 9 120 4201
Fragments arranged by size:
4201 951 826 260 259 120 15 9
Bpu10I CC'TnA_GC
Cuts at: 148 897 1777 2333 3484 3623 148
Size: 749 880 556 1151 139 3166
Fragments arranged by size:
3166 1151 880 749 556 139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1687 3817 4696 5188 5486 1687
Size: 2130 879 492 298 2842
Fragments arranged by size:
2842 2130 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1140 1803 6345 1140
Size: 663 4542 1436
Fragments arranged by size:
4542 1436 663
BsaAI yAC'GTr
Cuts at: 755 1302 1410 3994 755
Size: 547 108 2584 3402
Fragments arranged by size:
3402 2584 547 108
BsaBI GATnn'nnATC
Cuts at: 6 3094 4155 6192 6
Size: 3088 1061 2037 455
Fragments arranged by size:
3088 2037 1061 455
BsaHI Gr'CG_yC
Cuts at: 371 4072 4320 5022 6518 371
Size: 3701 248 702 1496 494
Fragments arranged by size:
3701 1496 702 494 248
BsaWI w'CCGG_w
Cuts at: 1765 3830 4351 5029 5303 5450 5861 1765
Size: 2065 521 678 274 147 411 2545
Fragments arranged by size:
2545 2065 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 289 319 397 427 1120 1150 1528 1558 2245
Size: 30 78 30 693 30 378 30 687
Cuts at: 2245 2275 3094 3124 289
Size: 30 819 30 3806
Fragments arranged by size:
3806 819 693 687 378 78 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 141 356 444 1262 1536 1851 1999 2547 2650
Size: 215 88 818 274 315 148 548 103
Cuts at: 2650 3213 3220 141
Size: 563 7 3562
Fragments arranged by size:
3562 818 563 548 315 274 215 148
103 88 7
BseYI C'CCAG_C
Cuts at: 283 890 965 1114 1118 1202 1506 1817 2844
Size: 607 75 149 4 84 304 311 1027
Cuts at: 2844 2875 3546 4066 4824 5401 5897 283
Size: 31 671 520 758 577 496 1027
Fragments arranged by size:
1027 1027 758 671 607 577 520 496
311 304 149 84 75 31 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1248 1324 2349 2459 3390 1248
Size: 76 1025 110 931 4499
Fragments arranged by size:
4499 1025 931 110 76
BsiEI CG_ry'CG
Cuts at: 104 2604 4183 4229 5437 104
Size: 2500 1579 46 1208 1308
Fragments arranged by size:
2500 1579 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 914 2381 2740 2836 3283 3473 3482 3655 4433
Size: 1467 359 96 447 190 9 173 778
Cuts at: 4433 4623 5415 914
Size: 190 792 2140
Fragments arranged by size:
2140 1467 792 778 447 359 190 190
173 96 9
BsmBI CGTCTCn'nnnn_
Cuts at: 219 643 1078 1744 1833 219
Size: 424 435 666 89 5027
Fragments arranged by size:
5027 666 435 424 89
Bsp1286I G_dGCh'C
Cuts at: 120 744 914 1274 1386 1533 1645 1907 1981
Size: 624 170 360 112 147 112 262 74
Cuts at: 1981 2381 2596 2620 2740 2836 3127 3212 3283
Size: 400 215 24 120 96 291 85 71
Cuts at: 3283 3425 3473 3482 3528 3655 4266 4359 4433
Size: 142 48 9 46 127 611 93 74
Cuts at: 4433 4623 4685 5415 120
Size: 190 62 730 1346
Fragments arranged by size:
1346 730 624 611 400 360 291 262
215 190 170 147 142 127 120 112
112 96 93 85 74 74 71 62
48 46 24 9
BspEI T'CCGG_A
Cuts at: 5861 5861
Size: 6641
BspHI T'CATG_A
Cuts at: 5817 5817
Size: 6641
BspMI ACCTGCnnnn'nnnn_
Cuts at: 827 2845 3364 3419 3691 3905 4207 4588 827
Size: 2018 519 55 272 214 302 381 2880
Fragments arranged by size:
2880 2018 519 381 302 272 214 55
BsrBI CCG'CTC
Cuts at: 50 305 866 1628 3347 4933 4987 6471 6636
Size: 255 561 762 1719 1586 54 1484 165
Cuts at: 6636 50
Size: 55
Fragments arranged by size:
1719 1586 1484 762 561 255 165 55
54
BsrDI GCAATG_nn'
Cuts at: 2334 2502 2514 4553 2334
Size: 168 12 2039 4422
Fragments arranged by size:
4422 2039 168 12
BsrFI r'CCGG_y
Cuts at: 429 906 3060 4639 4820 5029 429
Size: 477 2154 1579 181 209 2041
Fragments arranged by size:
2154 2041 1579 477 209 181
BsrGI T'GTAC_A
Cuts at: 2528 2528
Size: 6641
BssSI C'ACGA_G
Cuts at: 2202 4912 5270 2202
Size: 2710 358 3573
Fragments arranged by size:
3573 2710 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2452 4018 2452
Size: 1566 5075
BstBI TT'CG_AA
Cuts at: 944 5002 944
Size: 4058 2583
BstXI CCAn_nnnn'nTGG
Cuts at: 868 1346 1638 2418 2493 2547 868
Size: 478 292 780 75 54 4962
Fragments arranged by size:
4962 780 478 292 75 54
BstYI r'GATC_y
Cuts at: 1 3666 4133 4156 4491 4737 5749 5835 5847
Size: 3665 467 23 335 246 1012 86 12
Cuts at: 5847 6193 1
Size: 346 449
Fragments arranged by size:
3665 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1982 1982
Size: 6641
BtgI C'CryG_G
Cuts at: 401 824 2960 3161 3193 3390 401
Size: 423 2136 201 32 197 3652
Fragments arranged by size:
3652 2136 423 201 197 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1590 3272 3320 4591 4772 4935 1590
Size: 1682 48 1271 181 163 3296
Fragments arranged by size:
3296 1682 1271 181 163 48
BtsI GCAGTG_nn'
Cuts at: 287 883 1093 2658 2793 3884 287
Size: 596 210 1565 135 1091 3044
Fragments arranged by size:
3044 1565 1091 596 210 135
ClaI AT'CG_AT
Cuts at: 3047 3047
Size: 6641
DraI TTT'AAA
Cuts at: 3640 3640
Size: 6641
DrdI GACnn_nn'nnGTC
Cuts at: 201 2412 4347 5205 201
Size: 2211 1935 858 1637
Fragments arranged by size:
2211 1935 1637 858
EaeI y'GGCC_r
Cuts at: 489 1802 2601 3158 4226 4400 4791 4818 6163
Size: 1313 799 557 1068 174 391 27 1345
Cuts at: 6163 489
Size: 967
Fragments arranged by size:
1345 1313 1068 967 799 557 391 174
27
EagI C'GGCC_G
Cuts at: 2601 4226 2601
Size: 1625 5016
EarI CTCTTCn'nnn_
Cuts at: 358 427 1090 2939 4664 4874 358
Size: 69 663 1849 1725 210 2125
Fragments arranged by size:
2125 1849 1725 663 210 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1754 1854 4849 5159 5305 6451 1754
Size: 100 2995 310 146 1146 1944
Fragments arranged by size:
2995 1944 1146 310 146 100
EcoICRI GAG'CTC
Cuts at: 2379 2738 3653 2379
Size: 359 915 5367
Fragments arranged by size:
5367 915 359
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 144 229 403 1114 1354 1543 1614 1926 2440
Size: 85 174 711 240 189 71 312 514
Cuts at: 2440 2455 2464 2584 2871 3282 3307 4466 4898
Size: 15 9 120 287 411 25 1159 432
Cuts at: 4898 5645 5913 144
Size: 747 268 872
Fragments arranged by size:
1159 872 747 711 514 432 411 312
287 268 240 189 174 120 85 71
25 15 9
EcoNI CCTnn'n_nnAGG
Cuts at: 338 1558 338
Size: 1220 5421
EcoO109I rG'GnC_Cy
Cuts at: 132 378 567 741 839 1206 1271 1430 1458
Size: 246 189 174 98 367 65 159 28
Cuts at: 1458 1529 1642 2999 3123 3422 3524 3525 3780
Size: 71 113 1357 124 299 102 1 255
Cuts at: 3780 132
Size: 2993
Fragments arranged by size:
2993 1357 367 299 255 246 189 174
159 124 113 102 98 71 65 28
1
EcoRI G'AATT_C
Cuts at: 1005 3645 1005
Size: 2640 4001
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2481 3312 4660 2481
Size: 831 1348 4462
Fragments arranged by size:
4462 1348 831
FspI TGC'GCA
Cuts at: 2179 4422 2179
Size: 2243 4398
HaeII r_GCGC'y
Cuts at: 374 897 4075 4323 5345 6324 374
Size: 523 3178 248 1022 979 691
Fragments arranged by size:
3178 1022 979 691 523 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 397 429 1528 1560 4483 4513 4515 4545 397
Size: 32 1099 32 2923 30 2 30 2493
Fragments arranged by size:
2923 2493 1099 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2901 2973 3680 2901
Size: 72 707 5862
Fragments arranged by size:
5862 707 72
KasI G'GCGC_C
Cuts at: 370 4071 4319 370
Size: 3701 248 2692
Fragments arranged by size:
3701 2692 248
KpnI G_GTAC'C
Cuts at: 3661 3661
Size: 6641
MluI A'CGCG_T
Cuts at: 3500 6074 3500
Size: 2574 4067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1034 1206 1871 2090 2156 2917 3105 5312 5496
Size: 172 665 219 66 761 188 2207 184
Cuts at: 5496 1034
Size: 2179
Fragments arranged by size:
2207 2179 761 665 219 188 184 172
66
MscI TGG'CCA
Cuts at: 491 4402 491
Size: 3911 2730
MslI CAynn'nnrTG
Cuts at: 292 2507 3358 4630 4757 292
Size: 2215 851 1272 127 2176
Fragments arranged by size:
2215 2176 1272 851 127
MspA1I CmG'CkG
Cuts at: 313 1118 1224 1358 2058 2363 2864 3153 3163
Size: 805 106 134 700 305 501 289 10
Cuts at: 3163 3428 3434 3622 3752 4066 4426 5439 5684
Size: 265 6 188 130 314 360 1013 245
Cuts at: 5684 313
Size: 1270
Fragments arranged by size:
1270 1013 805 700 501 360 314 305
289 265 245 188 134 130 106 10
6
NaeI GCC'GGC
Cuts at: 431 4822 431
Size: 4391 2250
NarI GG'CG_CC
Cuts at: 371 4072 4320 371
Size: 3701 248 2692
Fragments arranged by size:
3701 2692 248
NcoI C'CATG_G
Cuts at: 2960 3390 2960
Size: 430 6211
NgoMIV G'CCGG_C
Cuts at: 429 4820 429
Size: 4391 2250
NsiI A_TGCA'T
Cuts at: 2158 2158
Size: 6641
NspI r_CATG'y
Cuts at: 181 3012 3694 4194 5101 181
Size: 2831 682 500 907 1721
Fragments arranged by size:
2831 1721 907 682 500
PasI CC'CwG_GG
Cuts at: 137 1274 1972 3528 137
Size: 1137 698 1556 3250
Fragments arranged by size:
3250 1556 1137 698
PciI A'CATG_T
Cuts at: 5097 5097
Size: 6641
PflMI CCAn_nnn'nTGG
Cuts at: 1332 2814 3156 1332
Size: 1482 342 4817
Fragments arranged by size:
4817 1482 342
PmeI GTTT'AAAC
Cuts at: 3640 3640
Size: 6641
PmlI CAC'GTG
Cuts at: 755 1302 1410 755
Size: 547 108 5986
Fragments arranged by size:
5986 547 108
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1120 1152 5780 5812 1120
Size: 32 4628 32 1949
Fragments arranged by size:
4628 1949 32 32
PpuMI rG'GwC_Cy
Cuts at: 132 378 567 839 1458 2999 132
Size: 246 189 272 619 1541 3774
Fragments arranged by size:
3774 1541 619 272 246 189
PshAI GACnn'nnGTC
Cuts at: 2494 2494
Size: 6641
PspOMI G'GGCC_C
Cuts at: 740 1270 1529 1641 3123 3421 3524 740
Size: 530 259 112 1482 298 103 3857
Fragments arranged by size:
3857 1482 530 298 259 112 103
PstI C_TGCA'G
Cuts at: 1034 1088 1264 3057 3433 3688 4373 1034
Size: 54 176 1793 376 255 685 3302
Fragments arranged by size:
3302 1793 685 376 255 176 54
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6641
PvuII CAG'CTG
Cuts at: 313 1118 1224 2058 2363 3153 3434 3622 4066
Size: 805 106 834 305 790 281 188 444
Cuts at: 4066 4426 313
Size: 360 2528
Fragments arranged by size:
2528 834 805 790 444 360 305 281
188 106
RsrII CG'GwC_CG
Cuts at: 4836 4836
Size: 6641
SacI G_AGCT'C
Cuts at: 2381 2740 3655 2381
Size: 359 915 5367
Fragments arranged by size:
5367 915 359
SacII CC_GC'GG
Cuts at: 3164 3164
Size: 6641
SalI G'TCGA_C
Cuts at: 3678 3678
Size: 6641
SanDI GG'GwC_CC
Cuts at: 132 567 1458 2999 132
Size: 435 891 1541 3774
Fragments arranged by size:
3774 1541 891 435
SapI GCTCTTCn'nnn_
Cuts at: 1090 4664 4874 1090
Size: 3574 210 2857
Fragments arranged by size:
3574 2857 210
SbfI CC_TGCA'GG
Cuts at: 3688 3688
Size: 6641
ScaI AGT'ACT
Cuts at: 6158 6158
Size: 6641
SexAI A'CCwGG_T
Cuts at: 1130 3326 1130
Size: 2196 4445
SfcI C'TryA_G
Cuts at: 33 1030 1038 1084 1260 3053 3333 3429 3684
Size: 997 8 46 176 1793 280 96 255
Cuts at: 3684 4369 5362 5553 33
Size: 685 993 191 1121
Fragments arranged by size:
1793 1121 997 993 685 280 255 191
176 96 46 8
SfiI GGCCn_nnn'nGGCC
Cuts at: 739 739
Size: 6641
SfoI GGC'GCC
Cuts at: 372 4073 4321 372
Size: 3701 248 2692
Fragments arranged by size:
3701 2692 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6641
SmaI CCC'GGG
Cuts at: 3663 3663
Size: 6641
SmlI C'TyrA_G
Cuts at: 1075 1666 2916 3796 4711 5203 5465 1075
Size: 591 1250 880 915 492 262 2251
Fragments arranged by size:
2251 1250 915 880 591 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6641
SphI G_CATG'C
Cuts at: 3694 4194 3694
Size: 500 6141
StuI AGG'CCT
Cuts at: 1019 2033 2686 3628 1019
Size: 1014 653 942 4032
Fragments arranged by size:
4032 1014 942 653
StyI C'CwwG_G
Cuts at: 447 1023 1312 1475 2037 2960 3390 3775 4129
Size: 576 289 163 562 923 430 385 354
Cuts at: 4129 447
Size: 2959
Fragments arranged by size:
2959 923 576 562 430 385 354 289
163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 129 2424 4224 4357 4816 5025 129
Size: 2295 1800 133 459 209 1745
Fragments arranged by size:
2295 1800 1745 459 209 133
TatI w'GTAC_w
Cuts at: 2318 2528 6156 2318
Size: 210 3628 2803
Fragments arranged by size:
3628 2803 210
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 289 321 6158 6190 289
Size: 32 5837 32 740
Fragments arranged by size:
5837 740 32 32
Tth111I GACn'n_nGTC
Cuts at: 3223 3328 4438 3223
Size: 105 1110 5426
Fragments arranged by size:
5426 1110 105
XbaI T'CTAG_A
Cuts at: 3672 3672
Size: 6641
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1442 2463 2665 3219 4136 1442
Size: 1021 202 554 917 3947
Fragments arranged by size:
3947 1021 917 554 202
XhoI C'TCGA_G
Cuts at: 1075 1075
Size: 6641
XmaI C'CCGG_G
Cuts at: 3661 3661
Size: 6641
XmnI GAAnn'nnTTC
Cuts at: 2884 3048 2884
Size: 164 6477
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AhdI AscI AvrII BaeI BmtI BsiWI BsmI
BssHII BstEII BstZ17I CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI PacI
PfoI PsiI PspXI PsrI SgrAI SnaBI SrfI SspI
SwaI ZraI