Restriction map for
pF1KE2461
(Circular) (Six-base) MAPSORT of: pF1KE2461.seq Check: 5449 from: 1 to: 4874
pF1KE2461 4874 bp
With 182 enzymes: SgfI *
March 15, 2013 17:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 252 252
Size: 4874
AccI GT'mk_AC
Cuts at: 1691 1912 1691
Size: 221 4653
Acc65I G'GTAC_C
Cuts at: 404 1341 1890 404
Size: 937 549 3388
Fragments arranged by size:
3388 937 549
AclI AA'CG_TT
Cuts at: 4714 4714
Size: 4874
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1620 2699 3131 3878 4146 1620
Size: 1079 432 747 268 2348
Fragments arranged by size:
2348 1079 747 432 268
AfeI AGC'GCT
Cuts at: 1477 1477
Size: 4874
AflIII A'CryG_T
Cuts at: 2226 3330 4307 2226
Size: 1104 977 2793
Fragments arranged by size:
2793 1104 977
AgeI A'CCGG_T
Cuts at: 3262 3262
Size: 4874
AleI CACnn'nnGTG
Cuts at: 1015 1129 1015
Size: 114 4760
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1723 1723
Size: 4874
AlwNI CAG_nnn'CTG
Cuts at: 845 2179 3746 845
Size: 1334 1567 1973
Fragments arranged by size:
1973 1567 1334
ApaLI G'TGCA_C
Cuts at: 1503 3644 1503
Size: 2141 2733
ApoI r'AATT_y
Cuts at: 1878 4227 4483 1878
Size: 2349 256 2269
Fragments arranged by size:
2349 2269 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4874
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4874
AvaI C'yCGr_G
Cuts at: 277 1894 277
Size: 1617 3257
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1325 1358 1325
Size: 33 4841
BamHI G'GATC_C
Cuts at: 1899 1899
Size: 4874
BanI G'GyrC_C
Cuts at: 404 597 819 857 978 1115 1341 1890 2304
Size: 193 222 38 121 137 226 549 414
Cuts at: 2304 2552 2587 404
Size: 248 35 2691
Fragments arranged by size:
2691 549 414 248 226 222 193 137
121 38 35
BanII G_rGCy'C
Cuts at: 494 589 1177 1888 2918 494
Size: 95 588 711 1030 2450
Fragments arranged by size:
2450 1030 711 588 95
BbeI G_GCGC'C
Cuts at: 2308 2556 2308
Size: 248 4626
BbsI GAAGACnn'nnnn_
Cuts at: 182 182
Size: 4874
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1737 1771 90
Size: 34 1613 34 3193
Fragments arranged by size:
3193 1613 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2772 3533 4874 2772
Size: 761 1341 2772
Fragments arranged by size:
2772 1341 761
BclI T'GATC_A
Cuts at: 1397 2394 1397
Size: 997 3877
BglI GCCn_nnn'nGGC
Cuts at: 134 1205 134
Size: 1071 3803
BglII A'GATC_T
Cuts at: 2389 4426 2389
Size: 2037 2837
BlpI GC'TnA_GC
Cuts at: 583 1404 1735 1986 583
Size: 821 331 251 3471
Fragments arranged by size:
3471 821 331 251
Bme1580I G_kGCm'C
Cuts at: 602 860 908 981 1507 2499 2592 3648 602
Size: 258 48 73 526 992 93 1056 1828
Fragments arranged by size:
1828 1056 992 526 258 93 73 48
BmrI ACTGGGnnnn_n'
Cuts at: 2086 2167 2502 4646 2086
Size: 81 335 2144 2314
Fragments arranged by size:
2314 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 238 523 697 1059 1533 3241 238
Size: 285 174 362 474 1708 1871
Fragments arranged by size:
1871 1708 474 362 285 174
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 746 746
Size: 4874
Bpu10I CC'TnA_GC
Cuts at: 1744 1744
Size: 4874
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1519 2050 2929 3421 3719 1519
Size: 531 879 492 298 2674
Fragments arranged by size:
2674 879 531 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 494 764 778 1504 4578 494
Size: 270 14 726 3074 790
Fragments arranged by size:
3074 790 726 270 14
BsaAI yAC'GTr
Cuts at: 1785 2227 1785
Size: 442 4432
BsaBI GATnn'nnATC
Cuts at: 6 2388 4425 6
Size: 2382 2037 455
Fragments arranged by size:
2382 2037 455
BsaHI Gr'CG_yC
Cuts at: 2305 2553 3255 4751 2305
Size: 248 702 1496 2428
Fragments arranged by size:
2428 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2063 2584 3262 3536 3683 4094 2063
Size: 521 678 274 147 411 2843
Fragments arranged by size:
2843 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 744 774 744
Size: 30 4844
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 373 1546 373
Size: 1173 3701
BseYI C'CCAG_C
Cuts at: 161 916 1170 1412 1729 2299 3057 3634 4130
Size: 755 254 242 317 570 758 577 496
Cuts at: 4130 161
Size: 905
Fragments arranged by size:
905 758 755 577 570 496 317 254
242
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 102 102
Size: 4874
BsiEI CG_ry'CG
Cuts at: 104 2416 2462 3670 104
Size: 2312 46 1208 1308
Fragments arranged by size:
2312 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 464 494 1040 1507 1823 1888 2666 2856 3648
Size: 30 546 467 316 65 778 190 792
Cuts at: 3648 464
Size: 1690
Fragments arranged by size:
1690 792 778 546 467 316 190 65
30
Bsp1286I G_dGCh'C
Cuts at: 464 494 589 602 860 908 981 1040 1177
Size: 30 95 13 258 48 73 59 137
Cuts at: 1177 1507 1823 1888 2499 2592 2666 2856 2918
Size: 330 316 65 611 93 74 190 62
Cuts at: 2918 3648 464
Size: 730 1690
Fragments arranged by size:
1690 730 611 330 316 258 190 137
95 93 74 73 65 62 59 48
30 13
BspEI T'CCGG_A
Cuts at: 4094 4094
Size: 4874
BspHI T'CATG_A
Cuts at: 4050 4050
Size: 4874
BspMI ACCTGCnnnn'nnnn_
Cuts at: 252 1006 1177 1715 1924 2138 2440 2821 252
Size: 754 171 538 209 214 302 381 2305
Fragments arranged by size:
2305 754 538 381 302 214 209 171
BsrBI CCG'CTC
Cuts at: 50 3166 3220 4704 4869 50
Size: 3116 54 1484 165 55
Fragments arranged by size:
3116 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2786 2786
Size: 4874
BsrFI r'CCGG_y
Cuts at: 2872 3053 3262 2872
Size: 181 209 4484
Fragments arranged by size:
4484 209 181
BssSI C'ACGA_G
Cuts at: 1848 3145 3503 1848
Size: 1297 358 3219
Fragments arranged by size:
3219 1297 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2251 2251
Size: 4874
BstBI TT'CG_AA
Cuts at: 3235 3235
Size: 4874
BstEII G'GTnAC_C
Cuts at: 282 282
Size: 4874
BstXI CCAn_nnnn'nTGG
Cuts at: 609 961 1381 1521 1725 609
Size: 352 420 140 204 3758
Fragments arranged by size:
3758 420 352 204 140
BstYI r'GATC_y
Cuts at: 1 617 675 1899 2366 2389 2724 2970 3982
Size: 616 58 1224 467 23 335 246 1012
Cuts at: 3982 4068 4080 4426 1
Size: 86 12 346 449
Fragments arranged by size:
1224 1012 616 467 449 346 335 246
86 58 23 12
Bsu36I CC'TnA_GG
Cuts at: 983 983
Size: 4874
BtgI C'CryG_G
Cuts at: 374 1374 1679 374
Size: 1000 305 3569
Fragments arranged by size:
3569 1000 305
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2824 3005 3168 2824
Size: 181 163 4530
Fragments arranged by size:
4530 181 163
BtsI GCAGTG_nn'
Cuts at: 539 885 1453 2117 539
Size: 346 568 664 3296
Fragments arranged by size:
3296 664 568 346
DraI TTT'AAA
Cuts at: 1873 1873
Size: 4874
DraIII CAC_nnn'GTG
Cuts at: 627 627
Size: 4874
DrdI GACnn_nn'nnGTC
Cuts at: 2580 3438 2580
Size: 858 4016
EaeI y'GGCC_r
Cuts at: 1682 1715 2459 2633 3024 3051 4396 1682
Size: 33 744 174 391 27 1345 2160
Fragments arranged by size:
2160 1345 744 391 174 33 27
EagI C'GGCC_G
Cuts at: 2459 2459
Size: 4874
EarI CTCTTCn'nnn_
Cuts at: 337 965 1651 2897 3107 337
Size: 628 686 1246 210 2104
Fragments arranged by size:
2104 1246 686 628 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3082 3392 3538 4684 3082
Size: 310 146 1146 3272
Fragments arranged by size:
3272 1146 310 146
EcoICRI GAG'CTC
Cuts at: 492 1886 492
Size: 1394 3480
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 746 1620 2699 3131 3878 4146 746
Size: 874 1079 432 747 268 1474
Fragments arranged by size:
1474 1079 874 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 334 1591 334
Size: 1257 3617
EcoO109I rG'GnC_Cy
Cuts at: 920 1251 2013 920
Size: 331 762 3781
Fragments arranged by size:
3781 762 331
EcoRI G'AATT_C
Cuts at: 1878 1878
Size: 4874
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 520 747 2893 520
Size: 227 2146 2501
Fragments arranged by size:
2501 2146 227
FspI TGC'GCA
Cuts at: 2655 2655
Size: 4874
HaeII r_GCGC'y
Cuts at: 1479 1857 2308 2556 3578 4557 1479
Size: 378 451 248 1022 979 1796
Fragments arranged by size:
1796 1022 979 451 378 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2716 2746 2748 2778 2716
Size: 30 2 30 4812
Fragments arranged by size:
4812 30 30 2
HincII GTy'rAC
Cuts at: 1913 1913
Size: 4874
KasI G'GCGC_C
Cuts at: 2304 2552 2304
Size: 248 4626
KpnI G_GTAC'C
Cuts at: 408 1345 1894 408
Size: 937 549 3388
Fragments arranged by size:
3388 937 549
MluI A'CGCG_T
Cuts at: 4307 4307
Size: 4874
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1332 3545 3729 1332
Size: 2213 184 2477
Fragments arranged by size:
2477 2213 184
MscI TGG'CCA
Cuts at: 2635 2635
Size: 4874
MslI CAynn'nnrTG
Cuts at: 1015 1129 2863 2990 1015
Size: 114 1734 127 2899
Fragments arranged by size:
2899 1734 127 114
MspA1I CmG'CkG
Cuts at: 411 1408 1416 1751 1985 2299 2659 3672 3917
Size: 997 8 335 234 314 360 1013 245
Cuts at: 3917 411
Size: 1368
Fragments arranged by size:
1368 1013 997 360 335 314 245 234
8
NaeI GCC'GGC
Cuts at: 3055 3055
Size: 4874
NarI GG'CG_CC
Cuts at: 2305 2553 2305
Size: 248 4626
NgoMIV G'CCGG_C
Cuts at: 3053 3053
Size: 4874
NspI r_CATG'y
Cuts at: 1927 2427 3334 1927
Size: 500 907 3467
Fragments arranged by size:
3467 907 500
PasI CC'CwG_GG
Cuts at: 399 1521 399
Size: 1122 3752
PciI A'CATG_T
Cuts at: 3330 3330
Size: 4874
PflMI CCAn_nnn'nTGG
Cuts at: 867 867
Size: 4874
PfoI T'CCnGG_A
Cuts at: 1660 1660
Size: 4874
PmeI GTTT'AAAC
Cuts at: 1873 1873
Size: 4874
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4013 4045 4013
Size: 32 4842
PpuMI rG'GwC_Cy
Cuts at: 920 920
Size: 4874
PstI C_TGCA'G
Cuts at: 143 263 1582 1921 2606 143
Size: 120 1319 339 685 2411
Fragments arranged by size:
2411 1319 685 339 120
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4874
PvuII CAG'CTG
Cuts at: 411 1416 2299 2659 411
Size: 1005 883 360 2626
Fragments arranged by size:
2626 1005 883 360
RsrII CG'GwC_CG
Cuts at: 3069 3069
Size: 4874
SacI G_AGCT'C
Cuts at: 494 1888 494
Size: 1394 3480
SalI G'TCGA_C
Cuts at: 1911 1911
Size: 4874
SapI GCTCTTCn'nnn_
Cuts at: 1651 2897 3107 1651
Size: 1246 210 3418
Fragments arranged by size:
3418 1246 210
SbfI CC_TGCA'GG
Cuts at: 1921 1921
Size: 4874
ScaI AGT'ACT
Cuts at: 4391 4391
Size: 4874
SexAI A'CCwGG_T
Cuts at: 815 815
Size: 4874
SfcI C'TryA_G
Cuts at: 33 139 259 945 1578 1917 2602 3595 3786
Size: 106 120 686 633 339 685 993 191
Cuts at: 3786 33
Size: 1121
Fragments arranged by size:
1121 993 686 685 633 339 191 120
106
SfoI GGC'GCC
Cuts at: 2306 2554 2306
Size: 248 4626
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4874
SmaI CCC'GGG
Cuts at: 1896 1896
Size: 4874
SmlI C'TyrA_G
Cuts at: 1498 2029 2944 3436 3698 1498
Size: 531 915 492 262 2674
Fragments arranged by size:
2674 915 531 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4874
SphI G_CATG'C
Cuts at: 1927 2427 1927
Size: 500 4374
StuI AGG'CCT
Cuts at: 233 1230 1831 233
Size: 997 601 3276
Fragments arranged by size:
3276 997 601
StyI C'CwwG_G
Cuts at: 495 1263 1337 2008 2362 495
Size: 768 74 671 354 3007
Fragments arranged by size:
3007 768 671 354 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1038 2457 2590 3049 3258 1038
Size: 1419 133 459 209 2654
Fragments arranged by size:
2654 1419 459 209 133
TatI w'GTAC_w
Cuts at: 4389 4389
Size: 4874
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 744 776 4391 4423 744
Size: 32 3615 32 1195
Fragments arranged by size:
3615 1195 32 32
Tth111I GACn'n_nGTC
Cuts at: 2671 2671
Size: 4874
XbaI T'CTAG_A
Cuts at: 1905 1905
Size: 4874
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 396 2369 396
Size: 1973 2901
XmaI C'CCGG_G
Cuts at: 1894 1894
Size: 4874
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI ApaI AscI AvrII BbvCI
BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI BssHII
BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI