Restriction map for
pF1KE2466
(Circular) (Six-base) MAPSORT of: pF1KE2466.seq Check: 2356 from: 1 to: 6071
pF1KE2466 6071 bp
With 182 enzymes: SgfI *
August 29, 2014 10:08 ..
AccI GT'mk_AC
Cuts at: 3109 3109
Size: 6071
Acc65I G'GTAC_C
Cuts at: 3087 3087
Size: 6071
AclI AA'CG_TT
Cuts at: 5911 5911
Size: 6071
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 255 649 1528 1966 2206 2212 3896 4328 5075
Size: 394 879 438 240 6 1684 432 747
Cuts at: 5075 5343 255
Size: 268 983
Fragments arranged by size:
1684 983 879 747 438 432 394 268
240 6
AfeI AGC'GCT
Cuts at: 481 481
Size: 6071
AflIII A'CryG_T
Cuts at: 880 2024 2564 3423 4527 5504 880
Size: 1144 540 859 1104 977 1447
Fragments arranged by size:
1447 1144 1104 977 859 540
AgeI A'CCGG_T
Cuts at: 4459 4459
Size: 6071
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2125 2125
Size: 6071
AlwNI CAG_nnn'CTG
Cuts at: 1271 2805 2937 2940 3376 4943 1271
Size: 1534 132 3 436 1567 2399
Fragments arranged by size:
2399 1567 1534 436 132 3
ApaLI G'TGCA_C
Cuts at: 4841 4841
Size: 6071
ApoI r'AATT_y
Cuts at: 1956 3075 5424 5680 1956
Size: 1119 2349 256 2347
Fragments arranged by size:
2349 2347 1119 256
AseI AT'TA_AT
Cuts at: 20 659 20
Size: 639 5432
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6071
AvaI C'yCGr_G
Cuts at: 327 782 2013 3091 327
Size: 455 1231 1078 3307
Fragments arranged by size:
3307 1231 1078 455
BamHI G'GATC_C
Cuts at: 3096 3096
Size: 6071
BanI G'GyrC_C
Cuts at: 3087 3501 3749 3784 3087
Size: 414 248 35 5374
Fragments arranged by size:
5374 414 248 35
BanII G_rGCy'C
Cuts at: 384 452 1285 2206 2270 2423 3085 4115 384
Size: 68 833 921 64 153 662 1030 2340
Fragments arranged by size:
2340 1030 921 833 662 153 68 64
BbeI G_GCGC'C
Cuts at: 3505 3753 3505
Size: 248 5823
BbsI GAAGACnn'nnnn_
Cuts at: 1155 1329 1341 2787 1155
Size: 174 12 1446 4439
Fragments arranged by size:
4439 1446 174 12
BciVI GTATCCnnnnn_n'
Cuts at: 3969 4730 6071 3969
Size: 761 1341 3969
Fragments arranged by size:
3969 1341 761
BclI T'GATC_A
Cuts at: 2303 3591 2303
Size: 1288 4783
BfrBI ATG'CAT
Cuts at: 2090 2090
Size: 6071
BglI GCCn_nnn'nGGC
Cuts at: 1363 1750 2767 2779 1363
Size: 387 1017 12 4655
Fragments arranged by size:
4655 1017 387 12
BglII A'GATC_T
Cuts at: 863 2687 3586 5623 863
Size: 1824 899 2037 1311
Fragments arranged by size:
2037 1824 1311 899
BlpI GC'TnA_GC
Cuts at: 737 1284 2098 3183 737
Size: 547 814 1085 3625
Fragments arranged by size:
3625 1085 814 547
Bme1580I G_kGCm'C
Cuts at: 214 1026 1246 3696 3789 4845 214
Size: 812 220 2450 93 1056 1440
Fragments arranged by size:
2450 1440 1056 812 220 93
BmgBI CAC'GTC
Cuts at: 881 2531 881
Size: 1650 4421
BmrI ACTGGGnnnn_n'
Cuts at: 2844 3283 3364 3699 5843 2844
Size: 439 81 335 2144 3072
Fragments arranged by size:
3072 2144 439 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 144 4438 144
Size: 4294 1777
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 547 553 685 2284 535
Size: 12 6 132 1599 4322
Fragments arranged by size:
4322 1599 132 12 6
Bpu10I CC'TnA_GC
Cuts at: 441 441
Size: 6071
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3247 4126 4618 4916 3247
Size: 879 492 298 4402
Fragments arranged by size:
4402 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 658 1674 5775 658
Size: 1016 4101 954
Fragments arranged by size:
4101 1016 954
BsaAI yAC'GTr
Cuts at: 1980 2025 3424 1980
Size: 45 1399 4627
Fragments arranged by size:
4627 1399 45
BsaBI GATnn'nnATC
Cuts at: 6 1132 2152 3585 5622 6
Size: 1126 1020 1433 2037 455
Fragments arranged by size:
2037 1433 1126 1020 455
BsaHI Gr'CG_yC
Cuts at: 424 1715 2010 2399 3059 3502 3750 4452 5948
Size: 1291 295 389 660 443 248 702 1496
Cuts at: 5948 424
Size: 547
Fragments arranged by size:
1496 1291 702 660 547 443 389 295
248
BsaWI w'CCGG_w
Cuts at: 335 3260 3781 4459 4733 4880 5291 335
Size: 2925 521 678 274 147 411 1115
Fragments arranged by size:
2925 1115 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1654 1684 1717 1747 2596 2626 2728 2758 1654
Size: 30 33 30 849 30 102 30 4967
Fragments arranged by size:
4967 849 102 33 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 508 1462 1567 1752 1930 2233 2604 2932 508
Size: 954 105 185 178 303 371 328 3647
Fragments arranged by size:
3647 954 371 328 303 185 178 105
BseYI C'CCAG_C
Cuts at: 1110 1201 1418 1647 2112 3030 3037 3496 4254
Size: 91 217 229 465 918 7 459 758
Cuts at: 4254 4831 5327 1110
Size: 577 496 1854
Fragments arranged by size:
1854 918 758 577 496 465 459 229
217 91 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 624 940 2704 376
Size: 248 316 1764 3743
Fragments arranged by size:
3743 1764 316 248
BsiEI CG_ry'CG
Cuts at: 104 143 282 3613 3659 4867 104
Size: 39 139 3331 46 1208 1308
Fragments arranged by size:
3331 1308 1208 139 46 39
BsiHKAI G_wGCw'C
Cuts at: 701 743 850 1285 1385 2423 2894 3085 3863
Size: 42 107 435 100 1038 471 191 778
Cuts at: 3863 4053 4845 701
Size: 190 792 1927
Fragments arranged by size:
1927 1038 792 778 471 435 191 190
107 100 42
Bsp1286I G_dGCh'C
Cuts at: 214 384 452 701 743 850 1026 1246 1285
Size: 170 68 249 42 107 176 220 39
Cuts at: 1285 1385 2206 2270 2423 2894 3085 3696 3789
Size: 100 821 64 153 471 191 611 93
Cuts at: 3789 3863 4053 4115 4845 214
Size: 74 190 62 730 1440
Fragments arranged by size:
1440 821 730 611 471 249 220 191
190 176 170 153 107 100 93 74
68 64 62 42 39
BspEI T'CCGG_A
Cuts at: 5291 5291
Size: 6071
BspHI T'CATG_A
Cuts at: 1682 2816 5247 1682
Size: 1134 2431 2506
Fragments arranged by size:
2506 2431 1134
BspMI ACCTGCnnnn'nnnn_
Cuts at: 206 1270 2684 3121 3335 3637 4018 206
Size: 1064 1414 437 214 302 381 2259
Fragments arranged by size:
2259 1414 1064 437 381 302 214
BsrBI CCG'CTC
Cuts at: 50 537 2964 4363 4417 5901 6066 50
Size: 487 2427 1399 54 1484 165 55
Fragments arranged by size:
2427 1484 1399 487 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3983 3983
Size: 6071
BsrFI r'CCGG_y
Cuts at: 281 1538 1997 2430 4069 4250 4459 281
Size: 1257 459 433 1639 181 209 1893
Fragments arranged by size:
1893 1639 1257 459 433 209 181
BsrGI T'GTAC_A
Cuts at: 883 1118 2027 2567 883
Size: 235 909 540 4387
Fragments arranged by size:
4387 909 540 235
BssSI C'ACGA_G
Cuts at: 466 912 2215 4342 4700 466
Size: 446 1303 2127 358 1837
Fragments arranged by size:
2127 1837 1303 446 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2937 3448 2937
Size: 511 5560
BstBI TT'CG_AA
Cuts at: 4432 4432
Size: 6071
BstXI CCAn_nnnn'nTGG
Cuts at: 1062 1377 1494 1062
Size: 315 117 5639
Fragments arranged by size:
5639 315 117
BstYI r'GATC_y
Cuts at: 1 714 863 2387 2687 2873 3096 3563 3586
Size: 713 149 1524 300 186 223 467 23
Cuts at: 3586 3921 4167 5179 5265 5277 5623 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1524 1012 713 467 449 346 335 300
246 223 186 149 86 23 12
BtgI C'CryG_G
Cuts at: 558 669 1184 1295 1331 1592 1736 2719 3051
Size: 111 515 111 36 261 144 983 332
Cuts at: 3051 558
Size: 3578
Fragments arranged by size:
3578 983 515 332 261 144 111 111
36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4021 4202 4365 4021
Size: 181 163 5727
Fragments arranged by size:
5727 181 163
BtsI GCAGTG_nn'
Cuts at: 952 1697 3314 952
Size: 745 1617 3709
Fragments arranged by size:
3709 1617 745
DraI TTT'AAA
Cuts at: 3070 3070
Size: 6071
DraIII CAC_nnn'GTG
Cuts at: 1241 1241
Size: 6071
DrdI GACnn_nn'nnGTC
Cuts at: 154 3777 4635 154
Size: 3623 858 1590
Fragments arranged by size:
3623 1590 858
EaeI y'GGCC_r
Cuts at: 140 279 923 1739 1751 3656 3830 4221 4248
Size: 139 644 816 12 1905 174 391 27
Cuts at: 4248 5593 140
Size: 1345 618
Fragments arranged by size:
1905 1345 816 644 618 391 174 139
27 12
EagI C'GGCC_G
Cuts at: 140 279 3656 140
Size: 139 3377 2555
Fragments arranged by size:
3377 2555 139
EarI CTCTTCn'nnn_
Cuts at: 754 865 1010 1321 1433 1439 2048 2224 4094
Size: 111 145 311 112 6 609 176 1870
Cuts at: 4094 4304 754
Size: 210 2521
Fragments arranged by size:
2521 1870 609 311 210 176 145 112
111 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2380 4279 4589 4735 5881 2380
Size: 1899 310 146 1146 2570
Fragments arranged by size:
2570 1899 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1283 2421 3083 1283
Size: 1138 662 4271
Fragments arranged by size:
4271 1138 662
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 255 535 547 553 649 685 1528 1966 2206
Size: 280 12 6 96 36 843 438 240
Cuts at: 2206 2212 2284 3896 4328 5075 5343 255
Size: 6 72 1612 432 747 268 983
Fragments arranged by size:
1612 983 843 747 438 432 280 268
240 96 72 36 12 6 6
EcoNI CCTnn'n_nnAGG
Cuts at: 514 1846 514
Size: 1332 4739
EcoO109I rG'GnC_Cy
Cuts at: 323 746 1422 1890 3210 323
Size: 423 676 468 1320 3184
Fragments arranged by size:
3184 1320 676 468 423
EcoRI G'AATT_C
Cuts at: 1956 3075 1956
Size: 1119 4952
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 840 4090 840
Size: 3250 2821
FspI TGC'GCA
Cuts at: 3852 3852
Size: 6071
HaeII r_GCGC'y
Cuts at: 483 1539 3505 3753 4775 5754 483
Size: 1056 1966 248 1022 979 800
Fragments arranged by size:
1966 1056 1022 979 800 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 358 390 424 456 1217 1249 3913 3943 3945
Size: 32 34 32 761 32 2664 30 2
Cuts at: 3945 3975 358
Size: 30 2454
Fragments arranged by size:
2664 2454 761 34 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 117 1559 2601 3110 117
Size: 1442 1042 509 3078
Fragments arranged by size:
3078 1442 1042 509
HindIII A'AGCT_T
Cuts at: 1908 2491 1908
Size: 583 5488
KasI G'GCGC_C
Cuts at: 3501 3749 3501
Size: 248 5823
KpnI G_GTAC'C
Cuts at: 3091 3091
Size: 6071
MluI A'CGCG_T
Cuts at: 5504 5504
Size: 6071
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1805 1986 2705 4742 4926 1805
Size: 181 719 2037 184 2950
Fragments arranged by size:
2950 2037 719 184 181
MscI TGG'CCA
Cuts at: 925 1753 3832 925
Size: 828 2079 3164
Fragments arranged by size:
3164 2079 828
MslI CAynn'nnrTG
Cuts at: 304 470 659 1126 1210 1375 1681 2107 2299
Size: 166 189 467 84 165 306 426 192
Cuts at: 2299 2815 4060 4187 304
Size: 516 1245 127 2188
Fragments arranged by size:
2188 1245 516 467 426 306 192 189
166 165 127 84
MspA1I CmG'CkG
Cuts at: 1418 2124 2241 2937 3182 3496 3856 4869 5114
Size: 706 117 696 245 314 360 1013 245
Cuts at: 5114 1418
Size: 2375
Fragments arranged by size:
2375 1013 706 696 360 314 245 245
117
NaeI GCC'GGC
Cuts at: 283 1540 4252 283
Size: 1257 2712 2102
Fragments arranged by size:
2712 2102 1257
NarI GG'CG_CC
Cuts at: 3502 3750 3502
Size: 248 5823
NcoI C'CATG_G
Cuts at: 1184 1592 1736 1184
Size: 408 144 5519
Fragments arranged by size:
5519 408 144
NgoMIV G'CCGG_C
Cuts at: 281 1538 4250 281
Size: 1257 2712 2102
Fragments arranged by size:
2712 2102 1257
NsiI A_TGCA'T
Cuts at: 2092 2092
Size: 6071
NspI r_CATG'y
Cuts at: 2568 3124 3624 4531 2568
Size: 556 500 907 4108
Fragments arranged by size:
4108 907 556 500
PciI A'CATG_T
Cuts at: 2564 4527 2564
Size: 1963 4108
PflMI CCAn_nnn'nTGG
Cuts at: 2805 2805
Size: 6071
PfoI T'CCnGG_A
Cuts at: 512 1841 2843 512
Size: 1329 1002 3740
Fragments arranged by size:
3740 1329 1002
PmeI GTTT'AAAC
Cuts at: 3070 3070
Size: 6071
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5210 5242 5210
Size: 32 6039
PpuMI rG'GwC_Cy
Cuts at: 1422 1890 1422
Size: 468 5603
PshAI GACnn'nnGTC
Cuts at: 343 343
Size: 6071
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 867 899 867
Size: 32 6039
PstI C_TGCA'G
Cuts at: 1267 2695 3118 3803 1267
Size: 1428 423 685 3535
Fragments arranged by size:
3535 1428 685 423
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6071
PvuII CAG'CTG
Cuts at: 2124 2937 3496 3856 2124
Size: 813 559 360 4339
Fragments arranged by size:
4339 813 559 360
RsrII CG'GwC_CG
Cuts at: 4266 4266
Size: 6071
SacI G_AGCT'C
Cuts at: 1285 2423 3085 1285
Size: 1138 662 4271
Fragments arranged by size:
4271 1138 662
SalI G'TCGA_C
Cuts at: 3108 3108
Size: 6071
SapI GCTCTTCn'nnn_
Cuts at: 4094 4304 4094
Size: 210 5861
SbfI CC_TGCA'GG
Cuts at: 3118 3118
Size: 6071
ScaI AGT'ACT
Cuts at: 127 5588 127
Size: 5461 610
SexAI A'CCwGG_T
Cuts at: 1061 2804 1061
Size: 1743 4328
SfcI C'TryA_G
Cuts at: 33 1263 1725 2691 3114 3799 4792 4983 33
Size: 1230 462 966 423 685 993 191 1121
Fragments arranged by size:
1230 1121 993 966 685 462 423 191
SfoI GGC'GCC
Cuts at: 3503 3751 3503
Size: 248 5823
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6071
SmaI CCC'GGG
Cuts at: 784 2015 3093 784
Size: 1231 1078 3762
Fragments arranged by size:
3762 1231 1078
SmlI C'TyrA_G
Cuts at: 3226 4141 4633 4895 3226
Size: 915 492 262 4402
Fragments arranged by size:
4402 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6071
SphI G_CATG'C
Cuts at: 3124 3624 3124
Size: 500 5571
SrfI GCCC'GGGC
Cuts at: 2015 2015
Size: 6071
StyI C'CwwG_G
Cuts at: 1184 1592 1736 1748 2747 2855 3205 3559 1184
Size: 408 144 12 999 108 350 354 3696
Fragments arranged by size:
3696 999 408 354 350 144 108 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1138 3654 3787 4246 4455 1138
Size: 2516 133 459 209 2754
Fragments arranged by size:
2754 2516 459 209 133
TatI w'GTAC_w
Cuts at: 125 266 883 896 1097 1118 2027 2291 2567
Size: 141 617 13 201 21 909 264 276
Cuts at: 2567 5586 125
Size: 3019 610
Fragments arranged by size:
3019 909 617 610 276 264 201 141
21 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2596 2628 5588 5620 2596
Size: 32 2960 32 3047
Fragments arranged by size:
3047 2960 32 32
Tth111I GACn'n_nGTC
Cuts at: 1063 2836 3868 1063
Size: 1773 1032 3266
Fragments arranged by size:
3266 1773 1032
XbaI T'CTAG_A
Cuts at: 3102 3102
Size: 6071
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 900 1977 2661 3566 900
Size: 1077 684 905 3405
Fragments arranged by size:
3405 1077 905 684
XmaI C'CCGG_G
Cuts at: 782 2013 3091 782
Size: 1231 1078 3762
Fragments arranged by size:
3762 1231 1078
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SrfI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BmtI BsiWI BsmI BsmBI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI PacI
PasI PmlI PsiI PspOMI PspXI SacII SanDI SfiI
SgrAI SnaBI SspI StuI SwaI XhoI XmnI ZraI