Restriction map for
pF1KE2469
(Circular) (Six-base) MAPSORT of: pF1KE2469.seq Check: 279 from: 1 to: 7859
pF1KE2469 7859 bp
With 182 enzymes: SgfI *
May 14, 2013 10:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2238 2238
Size: 7859
AatII G_ACGT'C
Cuts at: 1106 1106
Size: 7859
AccI GT'mk_AC
Cuts at: 473 4897 473
Size: 4424 3435
Acc65I G'GTAC_C
Cuts at: 2842 4875 2842
Size: 2033 5826
AclI AA'CG_TT
Cuts at: 2654 7699 2654
Size: 5045 2814
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1615 1654 3400 3741 4201 4213 4386 4507 4783
Size: 39 1746 341 460 12 173 121 276
Cuts at: 4783 5684 6116 6863 7131 1615
Size: 901 432 747 268 2343
Fragments arranged by size:
2343 1746 901 747 460 432 341 276
268 173 121 39 12
AflIII A'CryG_T
Cuts at: 4298 5211 6315 7292 4298
Size: 913 1104 977 4865
Fragments arranged by size:
4865 1104 977 913
AgeI A'CCGG_T
Cuts at: 6247 6247
Size: 7859
AhdI GACnn_n'nnGTC
Cuts at: 3063 3063
Size: 7859
AleI CACnn'nnGTG
Cuts at: 466 1669 3083 3592 466
Size: 1203 1414 509 4733
Fragments arranged by size:
4733 1414 1203 509
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1645 3101 4220 1645
Size: 1456 1119 5284
Fragments arranged by size:
5284 1456 1119
AlwNI CAG_nnn'CTG
Cuts at: 2103 2314 2787 3257 3681 4155 5164 6731 2103
Size: 211 473 470 424 474 1009 1567 3231
Fragments arranged by size:
3231 1567 1009 474 473 470 424 211
ApaI G_GGCC'C
Cuts at: 885 2008 885
Size: 1123 6736
ApaLI G'TGCA_C
Cuts at: 280 3276 4726 6629 280
Size: 2996 1450 1903 1510
Fragments arranged by size:
2996 1903 1510 1450
ApoI r'AATT_y
Cuts at: 4548 4863 7212 7468 4548
Size: 315 2349 256 4939
Fragments arranged by size:
4939 2349 315 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7859
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7859
AvaI C'yCGr_G
Cuts at: 313 727 959 1129 2063 2219 2630 2991 4586
Size: 414 232 170 934 156 411 361 1595
Cuts at: 4586 4879 313
Size: 293 3293
Fragments arranged by size:
3293 1595 934 414 411 361 293 232
170 156
BamHI G'GATC_C
Cuts at: 332 4884 332
Size: 4552 3307
BanI G'GyrC_C
Cuts at: 207 790 955 998 2028 2824 2842 4098 4408
Size: 583 165 43 1030 796 18 1256 310
Cuts at: 4408 4771 4875 5289 5537 5572 207
Size: 363 104 414 248 35 2494
Fragments arranged by size:
2494 1256 1030 796 583 414 363 310
248 165 104 43 35 18
BanII G_rGCy'C
Cuts at: 274 885 1994 2008 2637 3935 4028 4061 4094
Size: 611 1109 14 629 1298 93 33 33
Cuts at: 4094 4873 5903 274
Size: 779 1030 2230
Fragments arranged by size:
2230 1298 1109 1030 779 629 611 93
33 33 14
BbeI G_GCGC'C
Cuts at: 1002 5293 5541 1002
Size: 4291 248 3320
Fragments arranged by size:
4291 3320 248
BbsI GAAGACnn'nnnn_
Cuts at: 210 1098 2310 2652 2899 3150 4609 4818 210
Size: 888 1212 342 247 251 1459 209 3251
Fragments arranged by size:
3251 1459 1212 888 342 251 247 209
BbvCI CC'TCA_GC
Cuts at: 1009 3265 1009
Size: 2256 5603
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 4317 4351 90
Size: 34 4193 34 3598
Fragments arranged by size:
4193 3598 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2224 5757 6518 7859 2224
Size: 3533 761 1341 2224
Fragments arranged by size:
3533 2224 1341 761
BclI T'GATC_A
Cuts at: 5379 5379
Size: 7859
BglI GCCn_nnn'nGGC
Cuts at: 442 880 2087 3950 4250 442
Size: 438 1207 1863 300 4051
Fragments arranged by size:
4051 1863 1207 438 300
BglII A'GATC_T
Cuts at: 1355 5374 7411 1355
Size: 4019 2037 1803
Fragments arranged by size:
4019 2037 1803
BlpI GC'TnA_GC
Cuts at: 1936 4807 4971 1936
Size: 2871 164 4824
Fragments arranged by size:
4824 2871 164
Bme1580I G_kGCm'C
Cuts at: 284 320 885 949 960 1414 1983 2008 2033
Size: 36 565 64 11 454 569 25 25
Cuts at: 2033 3280 4313 4730 5484 5577 6633 284
Size: 1247 1033 417 754 93 1056 1510
Fragments arranged by size:
1510 1247 1056 1033 754 569 565 454
417 93 64 36 25 25 11
BmgBI CAC'GTC
Cuts at: 216 3353 4200 216
Size: 3137 847 3875
Fragments arranged by size:
3875 3137 847
BmrI ACTGGGnnnn_n'
Cuts at: 2105 2296 5071 5152 5487 7631 2105
Size: 191 2775 81 335 2144 2333
Fragments arranged by size:
2775 2333 2144 335 191 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 156 6226 156
Size: 6070 1789
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1594 2343 2944 3547 4012 1594
Size: 749 601 603 465 5441
Fragments arranged by size:
5441 749 603 601 465
Bpu10I CC'TnA_GC
Cuts at: 1009 3265 3535 4156 1009
Size: 2256 270 621 4712
Fragments arranged by size:
4712 2256 621 270
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 935 1287 2749 2833 3157 4801 5035 5914 6406
Size: 352 1462 84 324 1644 234 879 492
Cuts at: 6406 6704 935
Size: 298 2090
Fragments arranged by size:
2090 1644 1462 879 492 352 324 298
234 84
BsaI GGTCTCn'nnnn_
Cuts at: 2310 2555 3442 3984 7563 2310
Size: 245 887 542 3579 2606
Fragments arranged by size:
3579 2606 887 542 245
BsaAI yAC'GTr
Cuts at: 2168 5212 2168
Size: 3044 4815
BsaBI GATnn'nnATC
Cuts at: 6 2693 3483 4144 4234 5373 7410 6
Size: 2687 790 661 90 1139 2037 455
Fragments arranged by size:
2687 2037 1139 790 661 455 90
BsaHI Gr'CG_yC
Cuts at: 920 999 1103 3661 5290 5538 6240 7736 920
Size: 79 104 2558 1629 248 702 1496 1043
Fragments arranged by size:
2558 1629 1496 1043 702 248 104 79
BsaWI w'CCGG_w
Cuts at: 3416 5048 5569 6247 6521 6668 7079 3416
Size: 1632 521 678 274 147 411 4196
Fragments arranged by size:
4196 1632 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1487 1517 3109 3139 3787 3817 4159 4189 1487
Size: 30 1592 30 648 30 342 30 5157
Fragments arranged by size:
5157 1592 648 342 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 646 699 1286 1450 1984 2476 3037 3046 3064
Size: 53 587 164 534 492 561 9 18
Cuts at: 3064 646
Size: 5441
Fragments arranged by size:
5441 587 561 534 492 164 53 18
9
BseYI C'CCAG_C
Cuts at: 1041 1559 2007 2362 3695 4032 4481 5284 6042
Size: 518 448 355 1333 337 449 803 758
Cuts at: 6042 6619 7115 1041
Size: 577 496 1785
Fragments arranged by size:
1785 1333 803 758 577 518 496 449
448 355 337
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 1976 2135 3061 3382 3907 4336 4763 4865
Size: 1573 159 926 321 525 429 427 102
Cuts at: 4865 403
Size: 3397
Fragments arranged by size:
3397 1573 926 525 429 427 321 159
102
BsiEI CG_ry'CG
Cuts at: 104 146 2387 4245 5401 5447 6655 104
Size: 42 2241 1858 1156 46 1208 1308
Fragments arranged by size:
2241 1858 1308 1208 1156 46 42
BsiHKAI G_wGCw'C
Cuts at: 274 284 1570 2888 3280 3320 3890 3935 4028
Size: 10 1286 1318 392 40 570 45 93
Cuts at: 4028 4447 4730 4873 5651 5841 6633 274
Size: 419 283 143 778 190 792 1500
Fragments arranged by size:
1500 1318 1286 792 778 570 419 392
283 190 143 93 45 40 10
BsmI GAATG_Cn'
Cuts at: 2875 2875
Size: 7859
BsmBI CGTCTCn'nnnn_
Cuts at: 1669 1669
Size: 7859
Bsp1286I G_dGCh'C
Cuts at: 274 284 320 885 949 960 1414 1570 1983
Size: 10 36 565 64 11 454 156 413
Cuts at: 1983 1994 2008 2033 2637 2888 3280 3320 3890
Size: 11 14 25 604 251 392 40 570
Cuts at: 3890 3935 4028 4061 4094 4313 4447 4730 4873
Size: 45 93 33 33 219 134 283 143
Cuts at: 4873 5484 5577 5651 5841 5903 6633 274
Size: 611 93 74 190 62 730 1500
Fragments arranged by size:
1500 730 611 604 570 565 454 413
392 283 251 219 190 156 143 134
93 93 74 64 62 45 40 36
33 33 25 14 11 11 10
BspEI T'CCGG_A
Cuts at: 7079 7079
Size: 7859
BspHI T'CATG_A
Cuts at: 1742 2900 7035 1742
Size: 1158 4135 2566
Fragments arranged by size:
4135 2566 1158
BspMI ACCTGCnnnn'nnnn_
Cuts at: 332 743 1046 2238 2795 3680 4909 5123 5425
Size: 411 303 1192 557 885 1229 214 302
Cuts at: 5425 5806 332
Size: 381 2385
Fragments arranged by size:
2385 1229 1192 885 557 411 381 303
302 214
BsrBI CCG'CTC
Cuts at: 50 1590 2373 3542 6151 6205 7689 7854 50
Size: 1540 783 1169 2609 54 1484 165 55
Fragments arranged by size:
2609 1540 1484 1169 783 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1760 2807 3812 5771 1760
Size: 1047 1005 1959 3848
Fragments arranged by size:
3848 1959 1047 1005
BsrFI r'CCGG_y
Cuts at: 2086 2719 3130 4185 4249 5857 6038 6247 2086
Size: 633 411 1055 64 1608 181 209 3698
Fragments arranged by size:
3698 1608 1055 633 411 209 181 64
BsrGI T'GTAC_A
Cuts at: 425 425
Size: 7859
BssSI C'ACGA_G
Cuts at: 658 2978 3601 6130 6488 658
Size: 2320 623 2529 358 2029
Fragments arranged by size:
2529 2320 2029 623 358
BstAPI GCAn_nnn'nTGC
Cuts at: 324 4359 4725 5236 324
Size: 4035 366 511 2947
Fragments arranged by size:
4035 2947 511 366
BstBI TT'CG_AA
Cuts at: 4304 6220 4304
Size: 1916 5943
BstEII G'GTnAC_C
Cuts at: 399 3900 4707 399
Size: 3501 807 3551
Fragments arranged by size:
3551 3501 807
BstXI CCAn_nnnn'nTGG
Cuts at: 114 1421 4121 4647 114
Size: 1307 2700 526 3326
Fragments arranged by size:
3326 2700 1307 526
BstYI r'GATC_y
Cuts at: 1 332 1355 1961 3092 4139 4145 4235 4884
Size: 331 1023 606 1131 1047 6 90 649
Cuts at: 4884 5351 5374 5709 5955 6967 7053 7065 7411
Size: 467 23 335 246 1012 86 12 346
Cuts at: 7411 1
Size: 449
Fragments arranged by size:
1131 1047 1023 1012 649 606 467 449
346 335 331 246 90 86 23 12
6
Bsu36I CC'TnA_GG
Cuts at: 1705 2000 2035 1705
Size: 295 35 7529
Fragments arranged by size:
7529 295 35
BtgI C'CryG_G
Cuts at: 467 1331 1617 1829 2414 2437 2556 3097 3302
Size: 864 286 212 585 23 119 541 205
Cuts at: 3302 4053 4631 4652 467
Size: 751 578 21 3674
Fragments arranged by size:
3674 864 751 585 578 541 286 212
205 119 23 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 855 2138 3876 4088 4197 4320 4698 5809 5990
Size: 1283 1738 212 109 123 378 1111 181
Cuts at: 5990 6153 855
Size: 163 2561
Fragments arranged by size:
2561 1738 1283 1111 378 212 181 163
123 109
BtsI GCAGTG_nn'
Cuts at: 1981 2092 4260 4504 5102 1981
Size: 111 2168 244 598 4738
Fragments arranged by size:
4738 2168 598 244 111
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3441 3476 3441
Size: 35 7824
DraI TTT'AAA
Cuts at: 4858 4858
Size: 7859
DraIII CAC_nnn'GTG
Cuts at: 4460 4460
Size: 7859
DrdI GACnn_nn'nnGTC
Cuts at: 166 3292 5565 6423 166
Size: 3126 2273 858 1602
Fragments arranged by size:
3126 2273 1602 858
EaeI y'GGCC_r
Cuts at: 143 1031 1244 1538 1751 3191 3209 3386 3569
Size: 888 213 294 213 1440 18 177 183
Cuts at: 3569 3620 4123 4242 4649 5444 5618 6009 6036
Size: 51 503 119 407 795 174 391 27
Cuts at: 6036 7381 143
Size: 1345 621
Fragments arranged by size:
1440 1345 888 795 621 503 407 391
294 213 213 183 177 174 119 51
27 18
EagI C'GGCC_G
Cuts at: 143 4242 5444 143
Size: 4099 1202 2558
Fragments arranged by size:
4099 2558 1202
EarI CTCTTCn'nnn_
Cuts at: 232 253 358 1858 2398 3014 3023 3829 4405
Size: 21 105 1500 540 616 9 806 576
Cuts at: 4405 5882 6092 232
Size: 1477 210 1999
Fragments arranged by size:
1999 1500 1477 806 616 576 540 210
105 21 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3117 6067 6377 6523 7669 3117
Size: 2950 310 146 1146 3307
Fragments arranged by size:
3307 2950 1146 310 146
EcoICRI GAG'CTC
Cuts at: 272 3933 4026 4871 272
Size: 3661 93 845 3260
Fragments arranged by size:
3661 3260 845 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1594 1615 1654 2343 2944 3400 3547 3741 4012
Size: 21 39 689 601 456 147 194 271
Cuts at: 4012 4201 4213 4386 4507 4783 5684 6116 6863
Size: 189 12 173 121 276 901 432 747
Cuts at: 6863 7131 1594
Size: 268 2322
Fragments arranged by size:
2322 901 747 689 601 456 432 276
271 268 194 189 173 147 121 39
21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 661 993 1090 3007 3268 4596 4669 661
Size: 332 97 1917 261 1328 73 3851
Fragments arranged by size:
3851 1917 1328 332 261 97 73
EcoO109I rG'GnC_Cy
Cuts at: 168 732 881 882 1709 2004 2079 2358 2410
Size: 564 149 1 827 295 75 279 52
Cuts at: 2410 2970 3669 4389 4467 4998 168
Size: 560 699 720 78 531 3029
Fragments arranged by size:
3029 827 720 699 564 560 531 295
279 149 78 75 52 1
EcoRI G'AATT_C
Cuts at: 4863 4863
Size: 7859
EcoRV GAT'ATC
Cuts at: 2397 2691 2397
Size: 294 7565
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1177 4284 5878 1177
Size: 3107 1594 3158
Fragments arranged by size:
3158 3107 1594
FspI TGC'GCA
Cuts at: 5640 5640
Size: 7859
HaeII r_GCGC'y
Cuts at: 1002 3230 5293 5541 6563 7542 1002
Size: 2228 2063 248 1022 979 1319
Fragments arranged by size:
2228 2063 1319 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1347 1379 1487 1519 1892 1924 1933 1965 2662
Size: 32 108 32 373 32 9 32 697
Cuts at: 2662 2694 3049 3081 4214 4246 5701 5731 5733
Size: 32 355 32 1133 32 1455 30 2
Cuts at: 5733 5763 1347
Size: 30 3443
Fragments arranged by size:
3443 1455 1133 697 373 355 108 32
32 32 32 32 32 32 30 30
9 2
HincII GTy'rAC
Cuts at: 2608 4898 2608
Size: 2290 5569
HindIII A'AGCT_T
Cuts at: 3784 3784
Size: 7859
KasI G'GCGC_C
Cuts at: 998 5289 5537 998
Size: 4291 248 3320
Fragments arranged by size:
4291 3320 248
KpnI G_GTAC'C
Cuts at: 2846 4879 2846
Size: 2033 5826
MluI A'CGCG_T
Cuts at: 7292 7292
Size: 7859
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 521 1346 2222 3830 3938 4007 6530 6714 521
Size: 825 876 1608 108 69 2523 184 1666
Fragments arranged by size:
2523 1666 1608 876 825 184 108 69
MscI TGG'CCA
Cuts at: 1246 1540 3193 3211 3388 3622 4125 4651 5620
Size: 294 1653 18 177 234 503 526 969
Cuts at: 5620 1246
Size: 3485
Fragments arranged by size:
3485 1653 969 526 503 294 234 177
18
MslI CAynn'nnrTG
Cuts at: 466 679 874 1669 1741 2699 2812 3083 3592
Size: 213 195 795 72 958 113 271 509
Cuts at: 3592 4202 4645 5848 5975 466
Size: 610 443 1203 127 2350
Fragments arranged by size:
2350 1203 958 795 610 509 443 271
213 195 127 113 72
MspA1I CmG'CkG
Cuts at: 1501 1563 1619 1768 1875 2229 3040 3168 3681
Size: 62 56 149 107 354 811 128 513
Cuts at: 3681 3835 3898 4032 4350 4406 4485 4572 4970
Size: 154 63 134 318 56 79 87 398
Cuts at: 4970 5284 5644 6657 6902 1501
Size: 314 360 1013 245 2458
Fragments arranged by size:
2458 1013 811 513 398 360 354 318
314 245 154 149 134 128 107 87
79 63 62 56 56
NaeI GCC'GGC
Cuts at: 2721 3132 6040 2721
Size: 411 2908 4540
Fragments arranged by size:
4540 2908 411
NarI GG'CG_CC
Cuts at: 999 5290 5538 999
Size: 4291 248 3320
Fragments arranged by size:
4291 3320 248
NcoI C'CATG_G
Cuts at: 1331 2437 2556 3097 3302 4053 1331
Size: 1106 119 541 205 751 5137
Fragments arranged by size:
5137 1106 751 541 205 119
NgoMIV G'CCGG_C
Cuts at: 2719 3130 6038 2719
Size: 411 2908 4540
Fragments arranged by size:
4540 2908 411
NotI GC'GGCC_GC
Cuts at: 4242 4242
Size: 7859
NspI r_CATG'y
Cuts at: 432 3881 4912 5412 6319 432
Size: 3449 1031 500 907 1972
Fragments arranged by size:
3449 1972 1031 907 500
PasI CC'CwG_GG
Cuts at: 2967 2967
Size: 7859
PciI A'CATG_T
Cuts at: 6315 6315
Size: 7859
PflMI CCAn_nnn'nTGG
Cuts at: 1863 2151 2922 3845 4674 1863
Size: 288 771 923 829 5048
Fragments arranged by size:
5048 923 829 771 288
PfoI T'CCnGG_A
Cuts at: 2738 2738
Size: 7859
PmeI GTTT'AAAC
Cuts at: 4858 4858
Size: 7859
PmlI CAC'GTG
Cuts at: 2168 2168
Size: 7859
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3757 3789 6998 7030 3757
Size: 32 3209 32 4586
Fragments arranged by size:
4586 3209 32 32
PpuMI rG'GwC_Cy
Cuts at: 168 732 2358 3669 4389 168
Size: 564 1626 1311 720 3638
Fragments arranged by size:
3638 1626 1311 720 564
PshAI GACnn'nnGTC
Cuts at: 2758 2758
Size: 7859
PspOMI G'GGCC_C
Cuts at: 881 2004 881
Size: 1123 6736
PspXI vC'TCGA_Gb
Cuts at: 2991 2991
Size: 7859
PstI C_TGCA'G
Cuts at: 764 2099 2534 3011 3249 3325 3677 4388 4906
Size: 1335 435 477 238 76 352 711 518
Cuts at: 4906 5591 764
Size: 685 3032
Fragments arranged by size:
3032 1335 711 685 518 477 435 352
238 76
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7859
PvuII CAG'CTG
Cuts at: 1501 1768 2229 3040 3168 3681 3835 4032 4485
Size: 267 461 811 128 513 154 197 453
Cuts at: 4485 4572 5284 5644 1501
Size: 87 712 360 3716
Fragments arranged by size:
3716 811 712 513 461 453 360 267
197 154 128 87
RsrII CG'GwC_CG
Cuts at: 6054 6054
Size: 7859
SacI G_AGCT'C
Cuts at: 274 3935 4028 4873 274
Size: 3661 93 845 3260
Fragments arranged by size:
3661 3260 845 93
SacII CC_GC'GG
Cuts at: 1620 1620
Size: 7859
SalI G'TCGA_C
Cuts at: 4896 4896
Size: 7859
SapI GCTCTTCn'nnn_
Cuts at: 358 5882 6092 358
Size: 5524 210 2125
Fragments arranged by size:
5524 2125 210
SbfI CC_TGCA'GG
Cuts at: 4388 4906 4388
Size: 518 7341
ScaI AGT'ACT
Cuts at: 1531 3649 7376 1531
Size: 2118 3727 2014
Fragments arranged by size:
3727 2118 2014
SexAI A'CCwGG_T
Cuts at: 4748 4748
Size: 7859
SfcI C'TryA_G
Cuts at: 33 760 2095 2530 2779 3007 3245 3321 3673
Size: 727 1335 435 249 228 238 76 352
Cuts at: 3673 4384 4902 5587 6580 6771 33
Size: 711 518 685 993 191 1121
Fragments arranged by size:
1335 1121 993 727 711 685 518 435
352 249 238 228 191 76
SfiI GGCCn_nnn'nGGCC
Cuts at: 2087 3950 2087
Size: 1863 5996
SfoI GGC'GCC
Cuts at: 1000 5291 5539 1000
Size: 4291 248 3320
Fragments arranged by size:
4291 3320 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7859
SmaI CCC'GGG
Cuts at: 315 961 2065 4588 4881 315
Size: 646 1104 2523 293 3293
Fragments arranged by size:
3293 2523 1104 646 293
SmlI C'TyrA_G
Cuts at: 950 1266 2728 2848 2991 3172 4780 5014 5929
Size: 316 1462 120 143 181 1608 234 915
Cuts at: 5929 6421 6683 950
Size: 492 262 2126
Fragments arranged by size:
2126 1608 1462 915 492 316 262 234
181 143 120
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7859
SphI G_CATG'C
Cuts at: 4912 5412 4912
Size: 500 7359
SrfI GCCC'GGGC
Cuts at: 961 4588 961
Size: 3627 4232
StuI AGG'CCT
Cuts at: 913 4517 913
Size: 3604 4255
StyI C'CwwG_G
Cuts at: 275 1086 1331 2437 2556 3097 3302 3459 4053
Size: 811 245 1106 119 541 205 157 594
Cuts at: 4053 4131 4993 5347 275
Size: 78 862 354 2787
Fragments arranged by size:
2787 1106 862 811 594 541 354 245
205 157 119 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 418 1438 2401 3235 3709 5442 5575 6034 6243
Size: 1020 963 834 474 1733 133 459 209
Cuts at: 6243 418
Size: 2034
Fragments arranged by size:
2034 1733 1020 963 834 474 459 209
133
TatI w'GTAC_w
Cuts at: 425 1434 1529 3647 3866 7374 425
Size: 1009 95 2118 219 3508 910
Fragments arranged by size:
3508 2118 1009 910 219 95
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1862 1894 3502 3534 3581 3613 3684 3716 7376
Size: 32 1608 32 47 32 71 32 3660
Cuts at: 7376 7408 1862
Size: 32 2313
Fragments arranged by size:
3660 2313 1608 71 47 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 4393 4822 5656 4393
Size: 429 834 6596
Fragments arranged by size:
6596 834 429
XbaI T'CTAG_A
Cuts at: 4890 4890
Size: 7859
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1329 2572 3846 5354 1329
Size: 1243 1274 1508 3834
Fragments arranged by size:
3834 1508 1274 1243
XhoI C'TCGA_G
Cuts at: 2991 2991
Size: 7859
XmaI C'CCGG_G
Cuts at: 313 959 2063 4586 4879 313
Size: 646 1104 2523 293 3293
Fragments arranged by size:
3293 2523 1104 646 293
XmnI GAAnn'nnTTC
Cuts at: 811 1657 3649 811
Size: 846 1992 5021
Fragments arranged by size:
5021 1992 846
ZraI GAC'GTC
Cuts at: 1104 1104
Size: 7859
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AloI AscI AvrII BaeI BfrBI BmtI
BsiWI BssHII BstZ17I ClaI FseI FspAI HpaI MfeI
NdeI NheI NruI NsiI PacI PsiI PsrI SanDI
SgrAI SnaBI SspI SwaI