Restriction map for
pF1KE2473
(Circular) (Six-base) MAPSORT of: pF1KE2473.seq Check: 4483 from: 1 to: 5639
pF1KE2473 5639 bp
With 182 enzymes: SgfI *
December 2, 2014 13:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1172 1172
Size: 5639
AccI GT'mk_AC
Cuts at: 817 2677 817
Size: 1860 3779
Acc65I G'GTAC_C
Cuts at: 2655 2655
Size: 5639
AclI AA'CG_TT
Cuts at: 5479 5479
Size: 5639
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 1305 1881 2267 2302 2624 3464 3896 4643
Size: 968 576 386 35 322 840 432 747
Cuts at: 4643 4911 337
Size: 268 1065
Fragments arranged by size:
1065 968 840 747 576 432 386 322
268 35
AfeI AGC'GCT
Cuts at: 1581 2375 1581
Size: 794 4845
AflIII A'CryG_T
Cuts at: 1796 1931 2567 2991 4095 5072 1796
Size: 135 636 424 1104 977 2363
Fragments arranged by size:
2363 1104 977 636 424 135
AgeI A'CCGG_T
Cuts at: 4027 4027
Size: 5639
AleI CACnn'nnGTG
Cuts at: 199 624 199
Size: 425 5214
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2020 2368 2020
Size: 348 5291
AlwNI CAG_nnn'CTG
Cuts at: 513 1614 2944 4511 513
Size: 1101 1330 1567 1641
Fragments arranged by size:
1641 1567 1330 1101
ApaI G_GGCC'C
Cuts at: 146 411 726 1853 1978 146
Size: 265 315 1127 125 3807
Fragments arranged by size:
3807 1127 315 265 125
ApaLI G'TGCA_C
Cuts at: 1891 4409 1891
Size: 2518 3121
ApoI r'AATT_y
Cuts at: 2643 4992 5248 2643
Size: 2349 256 3034
Fragments arranged by size:
3034 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5639
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5639
AvaI C'yCGr_G
Cuts at: 401 872 1454 1627 2546 2659 401
Size: 471 582 173 919 113 3381
Fragments arranged by size:
3381 919 582 471 173 113
BamHI G'GATC_C
Cuts at: 1109 2664 1109
Size: 1555 4084
BanI G'GyrC_C
Cuts at: 460 951 996 1187 1196 1708 1750 2655 3069
Size: 491 45 191 9 512 42 905 414
Cuts at: 3069 3317 3352 460
Size: 248 35 2747
Fragments arranged by size:
2747 905 512 491 414 248 191 45
42 35 9
BanII G_rGCy'C
Cuts at: 146 411 726 920 1175 1628 1634 1853 1978
Size: 265 315 194 255 453 6 219 125
Cuts at: 1978 2653 3683 146
Size: 675 1030 2102
Fragments arranged by size:
2102 1030 675 453 315 265 255 219
194 125 6
BbeI G_GCGC'C
Cuts at: 464 1200 3073 3321 464
Size: 736 1873 248 2782
Fragments arranged by size:
2782 1873 736 248
BbsI GAAGACnn'nnnn_
Cuts at: 1029 2612 1029
Size: 1583 4056
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2424 2458 2424
Size: 34 5605
BciVI GTATCCnnnnn_n'
Cuts at: 2373 3537 4298 5639 2373
Size: 1164 761 1341 2373
Fragments arranged by size:
2373 1341 1164 761
BclI T'GATC_A
Cuts at: 3159 3159
Size: 5639
BglI GCCn_nnn'nGGC
Cuts at: 379 383 392 406 2236 2404 379
Size: 4 9 14 1830 168 3614
Fragments arranged by size:
3614 1830 168 14 9 4
BglII A'GATC_T
Cuts at: 3154 5191 3154
Size: 2037 3602
BlpI GC'TnA_GC
Cuts at: 1258 2287 2751 1258
Size: 1029 464 4146
Fragments arranged by size:
4146 1029 464
Bme1580I G_kGCm'C
Cuts at: 146 206 397 411 726 939 1237 1461 1853
Size: 60 191 14 315 213 298 224 392
Cuts at: 1853 1895 1978 2117 3264 3357 4413 146
Size: 42 83 139 1147 93 1056 1372
Fragments arranged by size:
1372 1147 1056 392 315 298 224 213
191 139 93 83 60 42 14
BmgBI CAC'GTC
Cuts at: 1130 1932 1130
Size: 802 4837
BmrI ACTGGGnnnn_n'
Cuts at: 735 2851 2932 3267 5411 735
Size: 2116 81 335 2144 963
Fragments arranged by size:
2144 2116 963 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 902 4006 902
Size: 3104 2535
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 716 1036 1862 466
Size: 250 320 826 4243
Fragments arranged by size:
4243 826 320 250
Bpu10I CC'TnA_GC
Cuts at: 2207 2207
Size: 5639
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1027 2317 2815 3694 4186 4484 1027
Size: 1290 498 879 492 298 2182
Fragments arranged by size:
2182 1290 879 498 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 559 696 721 2102 5343 559
Size: 137 25 1381 3241 855
Fragments arranged by size:
3241 1381 855 137 25
BsaAI yAC'GTr
Cuts at: 327 2992 327
Size: 2665 2974
BsaBI GATnn'nnATC
Cuts at: 6 3153 5190 6
Size: 3147 2037 455
Fragments arranged by size:
3147 2037 455
BsaHI Gr'CG_yC
Cuts at: 261 461 1197 3070 3318 4020 5516 261
Size: 200 736 1873 248 702 1496 384
Fragments arranged by size:
1873 1496 736 702 384 248 200
BsaWI w'CCGG_w
Cuts at: 2093 2828 3349 4027 4301 4448 4859 2093
Size: 735 521 678 274 147 411 2873
Fragments arranged by size:
2873 735 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1558 1588 1783 1813 1558
Size: 30 195 30 5384
Fragments arranged by size:
5384 195 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 553 1624 1831 553
Size: 1071 207 4361
Fragments arranged by size:
4361 1071 207
BseYI C'CCAG_C
Cuts at: 2179 3064 3822 4399 4895 2179
Size: 885 758 577 496 2923
Fragments arranged by size:
2923 885 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1138 1150 1138
Size: 12 5627
BsiEI CG_ry'CG
Cuts at: 104 2169 3181 3227 4435 104
Size: 2065 1012 46 1208 1308
Fragments arranged by size:
2065 1308 1208 1012 46
BsiHKAI G_wGCw'C
Cuts at: 905 1175 1703 1895 1999 2653 3431 3621 4413
Size: 270 528 192 104 654 778 190 792
Cuts at: 4413 905
Size: 2131
Fragments arranged by size:
2131 792 778 654 528 270 192 190
104
BsmBI CGTCTCn'nnnn_
Cuts at: 1136 1483 1136
Size: 347 5292
Bsp1286I G_dGCh'C
Cuts at: 146 206 397 411 726 905 920 939 1175
Size: 60 191 14 315 179 15 19 236
Cuts at: 1175 1237 1461 1628 1634 1703 1853 1895 1978
Size: 62 224 167 6 69 150 42 83
Cuts at: 1978 1999 2117 2653 3264 3357 3431 3621 3683
Size: 21 118 536 611 93 74 190 62
Cuts at: 3683 4413 146
Size: 730 1372
Fragments arranged by size:
1372 730 611 536 315 236 224 191
190 179 167 150 118 93 83 74
69 62 62 60 42 21 19 15
14 6
BspEI T'CCGG_A
Cuts at: 4859 4859
Size: 5639
BspHI T'CATG_A
Cuts at: 762 4815 762
Size: 4053 1586
BspMI ACCTGCnnnn'nnnn_
Cuts at: 645 747 1100 1172 2689 2903 3205 3586 645
Size: 102 353 72 1517 214 302 381 2698
Fragments arranged by size:
2698 1517 381 353 302 214 102 72
BsrBI CCG'CTC
Cuts at: 50 270 492 2274 2291 3931 3985 5469 5634
Size: 220 222 1782 17 1640 54 1484 165
Cuts at: 5634 50
Size: 55
Fragments arranged by size:
1782 1640 1484 222 220 165 55 54
17
BsrDI GCAATG_nn'
Cuts at: 524 751 2315 3551 524
Size: 227 1564 1236 2612
Fragments arranged by size:
2612 1564 1236 227
BsrFI r'CCGG_y
Cuts at: 304 373 457 1639 3637 3818 4027 304
Size: 69 84 1182 1998 181 209 1916
Fragments arranged by size:
1998 1916 1182 209 181 84 69
BsrGI T'GTAC_A
Cuts at: 1727 1727
Size: 5639
BssSI C'ACGA_G
Cuts at: 199 1168 1460 2530 3910 4268 199
Size: 969 292 1070 1380 358 1570
Fragments arranged by size:
1570 1380 1070 969 358 292
BstAPI GCAn_nnn'nTGC
Cuts at: 128 3016 128
Size: 2888 2751
BstBI TT'CG_AA
Cuts at: 4000 4000
Size: 5639
BstEII G'GTnAC_C
Cuts at: 2076 2076
Size: 5639
BstXI CCAn_nnnn'nTGG
Cuts at: 626 1060 2448 626
Size: 434 1388 3817
Fragments arranged by size:
3817 1388 434
BstYI r'GATC_y
Cuts at: 1 1109 2486 2664 3131 3154 3489 3735 4747
Size: 1108 1377 178 467 23 335 246 1012
Cuts at: 4747 4833 4845 5191 1
Size: 86 12 346 449
Fragments arranged by size:
1377 1108 1012 467 449 346 335 246
178 86 23 12
BtgI C'CryG_G
Cuts at: 107 146 2624 107
Size: 39 2478 3122
Fragments arranged by size:
3122 2478 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2130 3589 3770 3933 2130
Size: 1459 181 163 3836
Fragments arranged by size:
3836 1459 181 163
BtsI GCAGTG_nn'
Cuts at: 395 412 1177 1472 2882 395
Size: 17 765 295 1410 3152
Fragments arranged by size:
3152 1410 765 295 17
DraI TTT'AAA
Cuts at: 2638 2638
Size: 5639
DraIII CAC_nnn'GTG
Cuts at: 1406 1406
Size: 5639
DrdI GACnn_nn'nnGTC
Cuts at: 3345 4203 3345
Size: 858 4781
EaeI y'GGCC_r
Cuts at: 344 371 384 455 1292 2166 2414 3224 3398
Size: 27 13 71 837 874 248 810 174
Cuts at: 3398 3789 3816 5161 344
Size: 391 27 1345 822
Fragments arranged by size:
1345 874 837 822 810 391 248 174
71 27 27 13
EagI C'GGCC_G
Cuts at: 2166 3224 2166
Size: 1058 4581
EarI CTCTTCn'nnn_
Cuts at: 254 761 1987 3662 3872 254
Size: 507 1226 1675 210 2021
Fragments arranged by size:
2021 1675 1226 507 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 835 3847 4157 4303 5449 835
Size: 3012 310 146 1146 1025
Fragments arranged by size:
3012 1146 1025 310 146
EcoICRI GAG'CTC
Cuts at: 1173 2651 1173
Size: 1478 4161
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 466 716 1036 1305 1862 1881 2267 2302
Size: 129 250 320 269 557 19 386 35
Cuts at: 2302 2624 3464 3896 4643 4911 337
Size: 322 840 432 747 268 1065
Fragments arranged by size:
1065 840 747 557 432 386 322 320
269 268 250 129 35 19
EcoNI CCTnn'n_nnAGG
Cuts at: 1758 1844 1758
Size: 86 5553
EcoO109I rG'GnC_Cy
Cuts at: 142 407 408 723 1833 1849 1850 2778 142
Size: 265 1 315 1110 16 1 928 3003
Fragments arranged by size:
3003 1110 928 315 265 16 1 1
EcoRI G'AATT_C
Cuts at: 2643 2643
Size: 5639
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3658 3658
Size: 5639
FseI GG_CCGG'CC
Cuts at: 308 377 308
Size: 69 5570
FspI TGC'GCA
Cuts at: 1096 1656 3420 1096
Size: 560 1764 3315
Fragments arranged by size:
3315 1764 560
HaeII r_GCGC'y
Cuts at: 364 464 1200 1583 2377 3073 3321 4343 5322
Size: 100 736 383 794 696 248 1022 979
Cuts at: 5322 364
Size: 681
Fragments arranged by size:
1022 979 794 736 696 681 383 248
100
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 277 309 1212 1244 1529 1558 1561 1590 2020
Size: 32 903 32 285 29 3 29 430
Cuts at: 2020 2052 2353 2385 3481 3511 3513 3543 277
Size: 32 301 32 1096 30 2 30 2373
Fragments arranged by size:
2373 1096 903 430 301 285 32 32
32 32 30 30 29 29 3 2
HincII GTy'rAC
Cuts at: 2678 2678
Size: 5639
KasI G'GCGC_C
Cuts at: 460 1196 3069 3317 460
Size: 736 1873 248 2782
Fragments arranged by size:
2782 1873 736 248
KpnI G_GTAC'C
Cuts at: 2659 2659
Size: 5639
MfeI C'AATT_G
Cuts at: 1740 1740
Size: 5639
MluI A'CGCG_T
Cuts at: 5072 5072
Size: 5639
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4310 4494 4310
Size: 184 5455
MscI TGG'CCA
Cuts at: 346 2416 3400 346
Size: 2070 984 2585
Fragments arranged by size:
2585 2070 984
MslI CAynn'nnrTG
Cuts at: 199 624 1786 1945 2446 3628 3755 199
Size: 425 1162 159 501 1182 127 2083
Fragments arranged by size:
2083 1182 1162 501 425 159 127
MspA1I CmG'CkG
Cuts at: 277 336 586 894 1725 2179 2577 2750 3064
Size: 59 250 308 831 454 398 173 314
Cuts at: 3064 3424 4437 4682 277
Size: 360 1013 245 1234
Fragments arranged by size:
1234 1013 831 454 398 360 314 308
250 245 173 59
NaeI GCC'GGC
Cuts at: 306 375 459 1641 3820 306
Size: 69 84 1182 2179 2125
Fragments arranged by size:
2179 2125 1182 84 69
NarI GG'CG_CC
Cuts at: 461 1197 3070 3318 461
Size: 736 1873 248 2782
Fragments arranged by size:
2782 1873 736 248
NcoI C'CATG_G
Cuts at: 107 2624 107
Size: 2517 3122
NgoMIV G'CCGG_C
Cuts at: 304 373 457 1639 3818 304
Size: 69 84 1182 2179 2125
Fragments arranged by size:
2179 2125 1182 84 69
NspI r_CATG'y
Cuts at: 1329 1734 1800 2571 2692 3192 4099 1329
Size: 405 66 771 121 500 907 2869
Fragments arranged by size:
2869 907 771 500 405 121 66
PciI A'CATG_T
Cuts at: 1796 2567 4095 1796
Size: 771 1528 3340
Fragments arranged by size:
3340 1528 771
PflMI CCAn_nnn'nTGG
Cuts at: 780 1614 780
Size: 834 4805
PfoI T'CCnGG_A
Cuts at: 1839 1839
Size: 5639
PmeI GTTT'AAAC
Cuts at: 2638 2638
Size: 5639
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 923 955 4778 4810 923
Size: 32 3823 32 1752
Fragments arranged by size:
3823 1752 32 32
PpuMI rG'GwC_Cy
Cuts at: 1833 1833
Size: 5639
PspOMI G'GGCC_C
Cuts at: 142 407 722 1849 1974 142
Size: 265 315 1127 125 3807
Fragments arranged by size:
3807 1127 315 265 125
PspXI vC'TCGA_Gb
Cuts at: 872 872
Size: 5639
PstI C_TGCA'G
Cuts at: 259 335 893 1928 2217 2582 2686 3371 259
Size: 76 558 1035 289 365 104 685 2527
Fragments arranged by size:
2527 1035 685 558 365 289 104 76
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5639
PvuII CAG'CTG
Cuts at: 336 586 894 1725 2179 2577 3064 3424 336
Size: 250 308 831 454 398 487 360 2551
Fragments arranged by size:
2551 831 487 454 398 360 308 250
RsrII CG'GwC_CG
Cuts at: 3834 3834
Size: 5639
SacI G_AGCT'C
Cuts at: 1175 2653 1175
Size: 1478 4161
SalI G'TCGA_C
Cuts at: 2676 2676
Size: 5639
SapI GCTCTTCn'nnn_
Cuts at: 254 1987 3662 3872 254
Size: 1733 1675 210 2021
Fragments arranged by size:
2021 1733 1675 210
SbfI CC_TGCA'GG
Cuts at: 2686 2686
Size: 5639
ScaI AGT'ACT
Cuts at: 5156 5156
Size: 5639
SexAI A'CCwGG_T
Cuts at: 1265 1613 1265
Size: 348 5291
SfcI C'TryA_G
Cuts at: 33 255 331 889 963 1681 1758 1924 2213
Size: 222 76 558 74 718 77 166 289
Cuts at: 2213 2578 2682 3367 4360 4551 33
Size: 365 104 685 993 191 1121
Fragments arranged by size:
1121 993 718 685 558 365 289 222
191 166 104 77 76 74
SfiI GGCCn_nnn'nGGCC
Cuts at: 383 383
Size: 5639
SfoI GGC'GCC
Cuts at: 462 1198 3071 3319 462
Size: 736 1873 248 2782
Fragments arranged by size:
2782 1873 736 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5639
SmaI CCC'GGG
Cuts at: 403 1456 2661 403
Size: 1053 1205 3381
Fragments arranged by size:
3381 1205 1053
SmlI C'TyrA_G
Cuts at: 872 1042 2332 2794 3709 4201 4463 872
Size: 170 1290 462 915 492 262 2048
Fragments arranged by size:
2048 1290 915 492 462 262 170
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5639
SphI G_CATG'C
Cuts at: 2692 3192 2692
Size: 500 5139
StuI AGG'CCT
Cuts at: 2086 2086
Size: 5639
StyI C'CwwG_G
Cuts at: 107 236 1000 2624 2773 3127 107
Size: 129 764 1624 149 354 2619
Fragments arranged by size:
2619 1624 764 354 149 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 663 788 3222 3355 3814 4023 663
Size: 125 2434 133 459 209 2279
Fragments arranged by size:
2434 2279 459 209 133 125
TatI w'GTAC_w
Cuts at: 1072 1727 5154 1072
Size: 655 3427 1557
Fragments arranged by size:
3427 1557 655
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5156 5188 5156
Size: 32 5607
Tth111I GACn'n_nGTC
Cuts at: 734 2125 3436 734
Size: 1391 1311 2937
Fragments arranged by size:
2937 1391 1311
XbaI T'CTAG_A
Cuts at: 2670 2670
Size: 5639
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 626 2445 3134 114
Size: 512 1819 689 2619
Fragments arranged by size:
2619 1819 689 512
XhoI C'TCGA_G
Cuts at: 872 872
Size: 5639
XmaI C'CCGG_G
Cuts at: 401 1454 2659 401
Size: 1053 1205 3381
Fragments arranged by size:
3381 1205 1053
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BbvCI
BfrBI BmtI BsiWI BsmI BssHII BstZ17I Bsu36I ClaI
CspCI EcoRV FspAI HindIII HpaI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PsrI
SacII SanDI SgrAI SnaBI SrfI SspI SwaI XmnI
ZraI