Restriction map for
pF1KE2479
(Circular) (Six-base) MAPSORT of: pF1KE2479.seq Check: 1216 from: 1 to: 5657
pF1KE2479 5657 bp
With 182 enzymes: SgfI *
August 3, 2016 15:28 ..
AccI GT'mk_AC
Cuts at: 2483 2695 2483
Size: 212 5445
Acc65I G'GTAC_C
Cuts at: 2673 2673
Size: 5657
AclI AA'CG_TT
Cuts at: 5497 5497
Size: 5657
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 714 1371 1567 1742 2015 2293 3482 3914 4661
Size: 657 196 175 273 278 1189 432 747
Cuts at: 4661 4929 714
Size: 268 1442
Fragments arranged by size:
1442 1189 747 657 432 278 273 268
196 175
AfeI AGC'GCT
Cuts at: 232 232
Size: 5657
AflIII A'CryG_T
Cuts at: 2425 3009 4113 5090 2425
Size: 584 1104 977 2992
Fragments arranged by size:
2992 1104 977 584
AgeI A'CCGG_T
Cuts at: 4045 4045
Size: 5657
AlwNI CAG_nnn'CTG
Cuts at: 531 1366 2962 4529 531
Size: 835 1596 1567 1659
Fragments arranged by size:
1659 1596 1567 835
ApaI G_GGCC'C
Cuts at: 181 181
Size: 5657
ApaLI G'TGCA_C
Cuts at: 634 4427 634
Size: 3793 1864
ApoI r'AATT_y
Cuts at: 1238 2029 2661 5010 5266 1238
Size: 791 632 2349 256 1629
Fragments arranged by size:
2349 1629 791 632 256
AseI AT'TA_AT
Cuts at: 20 2117 20
Size: 2097 3560
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5657
AvaI C'yCGr_G
Cuts at: 744 2677 744
Size: 1933 3724
AvrII C'CTAG_G
Cuts at: 1489 1489
Size: 5657
BamHI G'GATC_C
Cuts at: 2682 2682
Size: 5657
BanI G'GyrC_C
Cuts at: 161 205 655 2673 3087 3335 3370 161
Size: 44 450 2018 414 248 35 2448
Fragments arranged by size:
2448 2018 450 414 248 44 35
BanII G_rGCy'C
Cuts at: 181 517 830 1475 2671 3701 181
Size: 336 313 645 1196 1030 2137
Fragments arranged by size:
2137 1196 1030 645 336 313
BbeI G_GCGC'C
Cuts at: 165 3091 3339 165
Size: 2926 248 2483
Fragments arranged by size:
2926 2483 248
BbsI GAAGACnn'nnnn_
Cuts at: 2573 2573
Size: 5657
BciVI GTATCCnnnnn_n'
Cuts at: 2041 3555 4316 5657 2041
Size: 1514 761 1341 2041
Fragments arranged by size:
2041 1514 1341 761
BclI T'GATC_A
Cuts at: 2022 3177 2022
Size: 1155 4502
BglI GCCn_nnn'nGGC
Cuts at: 778 850 778
Size: 72 5585
BglII A'GATC_T
Cuts at: 975 1382 1808 3172 5209 975
Size: 407 426 1364 2037 1423
Fragments arranged by size:
2037 1423 1364 426 407
BlpI GC'TnA_GC
Cuts at: 829 1750 2769 829
Size: 921 1019 3717
Fragments arranged by size:
3717 1019 921
Bme1580I G_kGCm'C
Cuts at: 181 638 3282 3375 4431 181
Size: 457 2644 93 1056 1407
Fragments arranged by size:
2644 1407 1056 457 93
BmrI ACTGGGnnnn_n'
Cuts at: 259 1398 2869 2950 3285 5429 259
Size: 1139 1471 81 335 2144 487
Fragments arranged by size:
2144 1471 1139 487 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1137 4024 1137
Size: 2887 2770
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 388 1018 2526 304
Size: 84 630 1508 3435
Fragments arranged by size:
3435 1508 630 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 2361 2833 3712 4204 4502 373
Size: 1988 472 879 492 298 1528
Fragments arranged by size:
1988 1528 879 492 472 298
BsaI GGTCTCn'nnnn_
Cuts at: 5361 5361
Size: 5657
BsaAI yAC'GTr
Cuts at: 450 3010 450
Size: 2560 3097
BsaBI GATnn'nnATC
Cuts at: 6 3171 5208 6
Size: 3165 2037 455
Fragments arranged by size:
3165 2037 455
BsaHI Gr'CG_yC
Cuts at: 162 242 419 3088 3336 4038 5534 162
Size: 80 177 2669 248 702 1496 285
Fragments arranged by size:
2669 1496 702 285 248 177 80
BsaWI w'CCGG_w
Cuts at: 2846 3367 4045 4319 4466 4877 2846
Size: 521 678 274 147 411 3626
Fragments arranged by size:
3626 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2202 2232 2202
Size: 30 5627
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 238 241 301 238
Size: 3 60 5594
Fragments arranged by size:
5594 60 3
BseYI C'CCAG_C
Cuts at: 1230 2632 3082 3840 4417 4913 1230
Size: 1402 450 758 577 496 1974
Fragments arranged by size:
1974 1402 758 577 496 450
BsiEI CG_ry'CG
Cuts at: 104 673 3199 3245 4453 104
Size: 569 2526 46 1208 1308
Fragments arranged by size:
2526 1308 1208 569 46
BsiHKAI G_wGCw'C
Cuts at: 365 638 830 1475 1751 2015 2671 3449 3639
Size: 273 192 645 276 264 656 778 190
Cuts at: 3639 4431 365
Size: 792 1591
Fragments arranged by size:
1591 792 778 656 645 276 273 264
192 190
BsmI GAATG_Cn'
Cuts at: 2408 2408
Size: 5657
BsmBI CGTCTCn'nnnn_
Cuts at: 123 508 123
Size: 385 5272
Bsp1286I G_dGCh'C
Cuts at: 181 365 517 638 830 1475 1751 2015 2671
Size: 184 152 121 192 645 276 264 656
Cuts at: 2671 3282 3375 3449 3639 3701 4431 181
Size: 611 93 74 190 62 730 1407
Fragments arranged by size:
1407 730 656 645 611 276 264 192
190 184 152 121 93 74 62
BspEI T'CCGG_A
Cuts at: 4877 4877
Size: 5657
BspHI T'CATG_A
Cuts at: 4833 4833
Size: 5657
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2707 2921 3223 3604 2707
Size: 214 302 381 4760
Fragments arranged by size:
4760 381 302 214
BsrBI CCG'CTC
Cuts at: 50 669 3949 4003 5487 5652 50
Size: 619 3280 54 1484 165 55
Fragments arranged by size:
3280 1484 619 165 55 54
BsrDI GCAATG_nn'
Cuts at: 907 1547 3569 907
Size: 640 2022 2995
Fragments arranged by size:
2995 2022 640
BsrFI r'CCGG_y
Cuts at: 158 3655 3836 4045 158
Size: 3497 181 209 1770
Fragments arranged by size:
3497 1770 209 181
BssHII G'CGCG_C
Cuts at: 406 406
Size: 5657
BssSI C'ACGA_G
Cuts at: 3928 4286 3928
Size: 358 5299
BstAPI GCAn_nnn'nTGC
Cuts at: 531 642 3034 531
Size: 111 2392 3154
Fragments arranged by size:
3154 2392 111
BstBI TT'CG_AA
Cuts at: 1331 4018 1331
Size: 2687 2970
BstEII G'GTnAC_C
Cuts at: 372 372
Size: 5657
BstXI CCAn_nnnn'nTGG
Cuts at: 603 603
Size: 5657
BstYI r'GATC_y
Cuts at: 1 930 975 1382 1808 2682 3149 3172 3507
Size: 929 45 407 426 874 467 23 335
Cuts at: 3507 3753 4765 4851 4863 5209 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 929 874 467 449 426 407 346
335 246 86 45 23 12
Bsu36I CC'TnA_GG
Cuts at: 1129 1129
Size: 5657
BtgI C'CryG_G
Cuts at: 107 201 107
Size: 94 5563
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3607 3788 3951 3607
Size: 181 163 5313
Fragments arranged by size:
5313 181 163
BtsI GCAGTG_nn'
Cuts at: 612 2900 612
Size: 2288 3369
DraI TTT'AAA
Cuts at: 2656 2656
Size: 5657
DrdI GACnn_nn'nnGTC
Cuts at: 1618 3363 4221 1618
Size: 1745 858 3054
Fragments arranged by size:
3054 1745 858
EaeI y'GGCC_r
Cuts at: 491 670 770 842 851 1196 3242 3416 3807
Size: 179 100 72 9 345 2046 174 391
Cuts at: 3807 3834 5179 491
Size: 27 1345 969
Fragments arranged by size:
2046 1345 969 391 345 179 174 100
72 27 9
EagI C'GGCC_G
Cuts at: 670 3242 670
Size: 2572 3085
EarI CTCTTCn'nnn_
Cuts at: 944 2365 3680 3890 944
Size: 1421 1315 210 2711
Fragments arranged by size:
2711 1421 1315 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3865 4175 4321 5467 3865
Size: 310 146 1146 4055
Fragments arranged by size:
4055 1146 310 146
EcoICRI GAG'CTC
Cuts at: 828 1473 2669 828
Size: 645 1196 3816
Fragments arranged by size:
3816 1196 645
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 388 714 1018 1371 1567 1742 2015 2293
Size: 84 326 304 353 196 175 273 278
Cuts at: 2293 2526 3482 3914 4661 4929 304
Size: 233 956 432 747 268 1032
Fragments arranged by size:
1032 956 747 432 353 326 304 278
273 268 233 196 175 84
EcoNI CCTnn'n_nnAGG
Cuts at: 1323 1323
Size: 5657
EcoO109I rG'GnC_Cy
Cuts at: 177 804 1486 2796 177
Size: 627 682 1310 3038
Fragments arranged by size:
3038 1310 682 627
EcoRI G'AATT_C
Cuts at: 2661 2661
Size: 5657
EcoRV GAT'ATC
Cuts at: 2538 2538
Size: 5657
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3676 3676
Size: 5657
FspI TGC'GCA
Cuts at: 274 454 3438 274
Size: 180 2984 2493
Fragments arranged by size:
2984 2493 180
FspAI rTGC'GCAy
Cuts at: 454 454
Size: 5657
HaeII r_GCGC'y
Cuts at: 165 234 3091 3339 4361 5340 165
Size: 69 2857 248 1022 979 482
Fragments arranged by size:
2857 1022 979 482 248 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 967 999 3499 3529 3531 3561 967
Size: 32 2500 30 2 30 3063
Fragments arranged by size:
3063 2500 32 30 30 2
HincII GTy'rAC
Cuts at: 309 2280 2696 309
Size: 1971 416 3270
Fragments arranged by size:
3270 1971 416
HindIII A'AGCT_T
Cuts at: 1016 1016
Size: 5657
HpaI GTT'AAC
Cuts at: 2280 2280
Size: 5657
KasI G'GCGC_C
Cuts at: 161 3087 3335 161
Size: 2926 248 2483
Fragments arranged by size:
2926 2483 248
KpnI G_GTAC'C
Cuts at: 2677 2677
Size: 5657
MluI A'CGCG_T
Cuts at: 5090 5090
Size: 5657
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4328 4512 4328
Size: 184 5473
MscI TGG'CCA
Cuts at: 772 844 853 1198 3418 772
Size: 72 9 345 2220 3011
Fragments arranged by size:
3011 2220 345 72 9
MslI CAynn'nnrTG
Cuts at: 139 265 3646 3773 139
Size: 126 3381 127 2023
Fragments arranged by size:
3381 2023 127 126
MspA1I CmG'CkG
Cuts at: 203 345 2768 3082 3442 4455 4700 203
Size: 142 2423 314 360 1013 245 1160
Fragments arranged by size:
2423 1160 1013 360 314 245 142
NaeI GCC'GGC
Cuts at: 160 3838 160
Size: 3678 1979
NarI GG'CG_CC
Cuts at: 162 3088 3336 162
Size: 2926 248 2483
Fragments arranged by size:
2926 2483 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5657
NgoMIV G'CCGG_C
Cuts at: 158 3836 158
Size: 3678 1979
NotI GC'GGCC_GC
Cuts at: 670 670
Size: 5657
NspI r_CATG'y
Cuts at: 642 1276 1893 2103 2640 2710 3210 4117 642
Size: 634 617 210 537 70 500 907 2182
Fragments arranged by size:
2182 907 634 617 537 500 210 70
PasI CC'CwG_GG
Cuts at: 1179 1179
Size: 5657
PciI A'CATG_T
Cuts at: 4113 4113
Size: 5657
PmeI GTTT'AAAC
Cuts at: 2656 2656
Size: 5657
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 853 885 4796 4828 853
Size: 32 3911 32 1682
Fragments arranged by size:
3911 1682 32 32
PpuMI rG'GwC_Cy
Cuts at: 804 1486 804
Size: 682 4975
PsiI TTA'TAA
Cuts at: 2455 2455
Size: 5657
PspOMI G'GGCC_C
Cuts at: 177 177
Size: 5657
PspXI vC'TCGA_Gb
Cuts at: 744 744
Size: 5657
PstI C_TGCA'G
Cuts at: 344 536 1808 2704 3389 344
Size: 192 1272 896 685 2612
Fragments arranged by size:
2612 1272 896 685 192
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5657
PvuII CAG'CTG
Cuts at: 345 3082 3442 345
Size: 2737 360 2560
Fragments arranged by size:
2737 2560 360
RsrII CG'GwC_CG
Cuts at: 3852 3852
Size: 5657
SacI G_AGCT'C
Cuts at: 830 1475 2671 830
Size: 645 1196 3816
Fragments arranged by size:
3816 1196 645
SacII CC_GC'GG
Cuts at: 204 204
Size: 5657
SalI G'TCGA_C
Cuts at: 2694 2694
Size: 5657
SapI GCTCTTCn'nnn_
Cuts at: 3680 3890 3680
Size: 210 5447
SbfI CC_TGCA'GG
Cuts at: 2704 2704
Size: 5657
ScaI AGT'ACT
Cuts at: 5174 5174
Size: 5657
SfcI C'TryA_G
Cuts at: 33 340 532 1804 1946 2700 3385 4378 4569
Size: 307 192 1272 142 754 685 993 191
Cuts at: 4569 33
Size: 1121
Fragments arranged by size:
1272 1121 993 754 685 307 192 191
142
SfiI GGCCn_nnn'nGGCC
Cuts at: 850 850
Size: 5657
SfoI GGC'GCC
Cuts at: 163 3089 3337 163
Size: 2926 248 2483
Fragments arranged by size:
2926 2483 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5657
SmaI CCC'GGG
Cuts at: 2679 2679
Size: 5657
SmlI C'TyrA_G
Cuts at: 388 744 2376 2812 3727 4219 4481 388
Size: 356 1632 436 915 492 262 1564
Fragments arranged by size:
1632 1564 915 492 436 356 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5657
SphI G_CATG'C
Cuts at: 2640 2710 3210 2640
Size: 70 500 5087
Fragments arranged by size:
5087 500 70
SspI AAT'ATT
Cuts at: 1982 2043 1982
Size: 61 5596
StuI AGG'CCT
Cuts at: 1127 1727 1127
Size: 600 5057
StyI C'CwwG_G
Cuts at: 107 1489 1914 2791 3145 107
Size: 1382 425 877 354 2619
Fragments arranged by size:
2619 1382 877 425 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 391 910 1417 3240 3373 3832 4041 391
Size: 519 507 1823 133 459 209 2007
Fragments arranged by size:
2007 1823 519 507 459 209 133
TatI w'GTAC_w
Cuts at: 5172 5172
Size: 5657
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5174 5206 5174
Size: 32 5625
Tth111I GACn'n_nGTC
Cuts at: 3454 3454
Size: 5657
XbaI T'CTAG_A
Cuts at: 2688 2688
Size: 5657
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3152 3152
Size: 5657
XhoI C'TCGA_G
Cuts at: 744 744
Size: 5657
XmaI C'CCGG_G
Cuts at: 2677 2677
Size: 5657
XmnI GAAnn'nnTTC
Cuts at: 868 962 868
Size: 94 5563
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PasI PciI PmeI
PpiI PpuMI PsiI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsgI
BsiWI BsrGI BstZ17I ClaI CspCI DraIII FseI MfeI
NdeI NheI NruI NsiI PacI PflMI PfoI PmlI
PshAI PsrI SanDI SexAI SgrAI SnaBI SrfI SwaI
ZraI