Restriction map for
pF1KE2494
(Circular) (Six-base) MAPSORT of: pF1KE2494.seq Check: 8092 from: 1 to: 4283
pF1KE2494 4283 bp
With 182 enzymes: SgfI *
December 2, 2013 11:07 ..
AccI GT'mk_AC
Cuts at: 416 1321 416
Size: 905 3378
Acc65I G'GTAC_C
Cuts at: 1299 1299
Size: 4283
AclI AA'CG_TT
Cuts at: 4123 4123
Size: 4283
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 482 781 910 2108 2540 3287 3555 482
Size: 299 129 1198 432 747 268 1210
Fragments arranged by size:
1210 1198 747 432 299 268 129
AflIII A'CryG_T
Cuts at: 1635 2739 3716 1635
Size: 1104 977 2202
Fragments arranged by size:
2202 1104 977
AgeI A'CCGG_T
Cuts at: 2671 2671
Size: 4283
AleI CACnn'nnGTG
Cuts at: 535 535
Size: 4283
AlwNI CAG_nnn'CTG
Cuts at: 1588 3155 1588
Size: 1567 2716
ApaI G_GGCC'C
Cuts at: 116 116
Size: 4283
ApaLI G'TGCA_C
Cuts at: 3053 3053
Size: 4283
ApoI r'AATT_y
Cuts at: 1287 3636 3892 1287
Size: 2349 256 1678
Fragments arranged by size:
2349 1678 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4283
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4283
AvaI C'yCGr_G
Cuts at: 313 1303 313
Size: 990 3293
BamHI G'GATC_C
Cuts at: 1308 1308
Size: 4283
BanI G'GyrC_C
Cuts at: 1299 1713 1961 1996 1299
Size: 414 248 35 3586
Fragments arranged by size:
3586 414 248 35
BanII G_rGCy'C
Cuts at: 116 1297 2327 116
Size: 1181 1030 2072
Fragments arranged by size:
2072 1181 1030
BbeI G_GCGC'C
Cuts at: 1717 1965 1717
Size: 248 4035
BbsI GAAGACnn'nnnn_
Cuts at: 395 1099 395
Size: 704 3579
BbvCI CC'TCA_GC
Cuts at: 692 1199 692
Size: 507 3776
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 505 539 505
Size: 34 4249
BciVI GTATCCnnnnn_n'
Cuts at: 2181 2942 4283 2181
Size: 761 1341 2181
Fragments arranged by size:
2181 1341 761
BclI T'GATC_A
Cuts at: 236 1803 236
Size: 1567 2716
BglII A'GATC_T
Cuts at: 1032 1798 3835 1032
Size: 766 2037 1480
Fragments arranged by size:
2037 1480 766
BlpI GC'TnA_GC
Cuts at: 1395 1395
Size: 4283
Bme1580I G_kGCm'C
Cuts at: 116 1908 2001 3057 116
Size: 1792 93 1056 1342
Fragments arranged by size:
1792 1342 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1095 1495 1576 1911 4055 1095
Size: 400 81 335 2144 1323
Fragments arranged by size:
2144 1323 400 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2650 2650
Size: 4283
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 1228 1240 1252 385
Size: 843 12 12 3416
Fragments arranged by size:
3416 843 12 12
Bpu10I CC'TnA_GC
Cuts at: 692 1199 692
Size: 507 3776
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 982 1459 2338 2830 3128 982
Size: 477 879 492 298 2137
Fragments arranged by size:
2137 879 492 477 298
BsaI GGTCTCn'nnnn_
Cuts at: 904 3987 904
Size: 3083 1200
BsaAI yAC'GTr
Cuts at: 1636 1636
Size: 4283
BsaBI GATnn'nnATC
Cuts at: 6 1797 3834 6
Size: 1791 2037 455
Fragments arranged by size:
2037 1791 455
BsaHI Gr'CG_yC
Cuts at: 1714 1962 2664 4160 1714
Size: 248 702 1496 1837
Fragments arranged by size:
1837 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1472 1993 2671 2945 3092 3503 1472
Size: 521 678 274 147 411 2252
Fragments arranged by size:
2252 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 482 512 889 919 1081 1111 482
Size: 30 377 30 162 30 3654
Fragments arranged by size:
3654 377 162 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 286 367 709 853 874 1195 1216 286
Size: 81 342 144 21 321 21 3353
Fragments arranged by size:
3353 342 321 144 81 21 21
BseYI C'CCAG_C
Cuts at: 1708 2466 3043 3539 1708
Size: 758 577 496 2452
Fragments arranged by size:
2452 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1825 1871 3079 104
Size: 1721 46 1208 1308
Fragments arranged by size:
1721 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 866 1297 2075 2265 3057 866
Size: 431 778 190 792 2092
Fragments arranged by size:
2092 792 778 431 190
Bsp1286I G_dGCh'C
Cuts at: 116 866 1297 1908 2001 2075 2265 2327 3057
Size: 750 431 611 93 74 190 62 730
Cuts at: 3057 116
Size: 1342
Fragments arranged by size:
1342 750 730 611 431 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3503 3503
Size: 4283
BspHI T'CATG_A
Cuts at: 3459 3459
Size: 4283
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1196 1333 1547 1849 2230 1196
Size: 137 214 302 381 3249
Fragments arranged by size:
3249 381 302 214 137
BsrBI CCG'CTC
Cuts at: 50 2575 2629 4113 4278 50
Size: 2525 54 1484 165 55
Fragments arranged by size:
2525 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2195 2195
Size: 4283
BsrFI r'CCGG_y
Cuts at: 2281 2462 2671 2281
Size: 181 209 3893
Fragments arranged by size:
3893 209 181
BssSI C'ACGA_G
Cuts at: 2554 2912 2554
Size: 358 3925
BstAPI GCAn_nnn'nTGC
Cuts at: 1660 1660
Size: 4283
BstBI TT'CG_AA
Cuts at: 2644 2644
Size: 4283
BstYI r'GATC_y
Cuts at: 1 1032 1308 1775 1798 2133 2379 3391 3477
Size: 1031 276 467 23 335 246 1012 86
Cuts at: 3477 3489 3835 1
Size: 12 346 449
Fragments arranged by size:
1031 1012 467 449 346 335 276 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 880 880
Size: 4283
BtgI C'CryG_G
Cuts at: 107 107
Size: 4283
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2233 2414 2577 2233
Size: 181 163 3939
Fragments arranged by size:
3939 181 163
BtsI GCAGTG_nn'
Cuts at: 142 1526 142
Size: 1384 2899
DraI TTT'AAA
Cuts at: 1282 1282
Size: 4283
DraIII CAC_nnn'GTG
Cuts at: 945 945
Size: 4283
DrdI GACnn_nn'nnGTC
Cuts at: 174 1989 2847 174
Size: 1815 858 1610
Fragments arranged by size:
1815 1610 858
EaeI y'GGCC_r
Cuts at: 440 1868 2042 2433 2460 3805 440
Size: 1428 174 391 27 1345 918
Fragments arranged by size:
1428 1345 918 391 174 27
EagI C'GGCC_G
Cuts at: 1868 1868
Size: 4283
EarI CTCTTCn'nnn_
Cuts at: 794 830 2306 2516 794
Size: 36 1476 210 2561
Fragments arranged by size:
2561 1476 210 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2491 2801 2947 4093 2491
Size: 310 146 1146 2681
Fragments arranged by size:
2681 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1295 1295
Size: 4283
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 482 781 910 1228 1240 1252 2108 2540
Size: 97 299 129 318 12 12 856 432
Cuts at: 2540 3287 3555 385
Size: 747 268 1113
Fragments arranged by size:
1113 856 747 432 318 299 268 129
97 12 12
EcoO109I rG'GnC_Cy
Cuts at: 112 564 1185 1422 112
Size: 452 621 237 2973
Fragments arranged by size:
2973 621 452 237
EcoRI G'AATT_C
Cuts at: 1287 1287
Size: 4283
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2302 2302
Size: 4283
FspI TGC'GCA
Cuts at: 2064 2064
Size: 4283
HaeII r_GCGC'y
Cuts at: 1717 1965 2987 3966 1717
Size: 248 1022 979 2034
Fragments arranged by size:
2034 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 313 345 482 514 2125 2155 2157 2187 313
Size: 32 137 32 1611 30 2 30 2409
Fragments arranged by size:
2409 1611 137 32 32 30 30 2
HincII GTy'rAC
Cuts at: 849 1322 849
Size: 473 3810
KasI G'GCGC_C
Cuts at: 1713 1961 1713
Size: 248 4035
KpnI G_GTAC'C
Cuts at: 1303 1303
Size: 4283
MluI A'CGCG_T
Cuts at: 3716 3716
Size: 4283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2954 3138 2954
Size: 184 4099
MscI TGG'CCA
Cuts at: 442 2044 442
Size: 1602 2681
MslI CAynn'nnrTG
Cuts at: 535 2272 2399 535
Size: 1737 127 2419
Fragments arranged by size:
2419 1737 127
MspA1I CmG'CkG
Cuts at: 436 691 1394 1708 2068 3081 3326 436
Size: 255 703 314 360 1013 245 1393
Fragments arranged by size:
1393 1013 703 360 314 255 245
NaeI GCC'GGC
Cuts at: 2464 2464
Size: 4283
NarI GG'CG_CC
Cuts at: 1714 1962 1714
Size: 248 4035
NcoI C'CATG_G
Cuts at: 107 107
Size: 4283
NgoMIV G'CCGG_C
Cuts at: 2462 2462
Size: 4283
NspI r_CATG'y
Cuts at: 477 1336 1836 2743 477
Size: 859 500 907 2017
Fragments arranged by size:
2017 907 859 500
PciI A'CATG_T
Cuts at: 2739 2739
Size: 4283
PflMI CCAn_nnn'nTGG
Cuts at: 945 945
Size: 4283
PfoI T'CCnGG_A
Cuts at: 362 1229 362
Size: 867 3416
PmeI GTTT'AAAC
Cuts at: 1282 1282
Size: 4283
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3422 3454 3422
Size: 32 4251
PpuMI rG'GwC_Cy
Cuts at: 564 1185 564
Size: 621 3662
PspOMI G'GGCC_C
Cuts at: 112 112
Size: 4283
PstI C_TGCA'G
Cuts at: 602 1330 2015 602
Size: 728 685 2870
Fragments arranged by size:
2870 728 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4283
PvuII CAG'CTG
Cuts at: 436 691 1708 2068 436
Size: 255 1017 360 2651
Fragments arranged by size:
2651 1017 360 255
RsrII CG'GwC_CG
Cuts at: 2478 2478
Size: 4283
SacI G_AGCT'C
Cuts at: 1297 1297
Size: 4283
SalI G'TCGA_C
Cuts at: 1320 1320
Size: 4283
SapI GCTCTTCn'nnn_
Cuts at: 794 2306 2516 794
Size: 1512 210 2561
Fragments arranged by size:
2561 1512 210
SbfI CC_TGCA'GG
Cuts at: 1330 1330
Size: 4283
ScaI AGT'ACT
Cuts at: 3800 3800
Size: 4283
SfcI C'TryA_G
Cuts at: 33 598 1326 2011 3004 3195 33
Size: 565 728 685 993 191 1121
Fragments arranged by size:
1121 993 728 685 565 191
SfoI GGC'GCC
Cuts at: 1715 1963 1715
Size: 248 4035
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4283
SmaI CCC'GGG
Cuts at: 1305 1305
Size: 4283
SmlI C'TyrA_G
Cuts at: 997 1438 2353 2845 3107 997
Size: 441 915 492 262 2173
Fragments arranged by size:
2173 915 492 441 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4283
SphI G_CATG'C
Cuts at: 1336 1836 1336
Size: 500 3783
SspI AAT'ATT
Cuts at: 615 615
Size: 4283
StuI AGG'CCT
Cuts at: 328 455 790 328
Size: 127 335 3821
Fragments arranged by size:
3821 335 127
StyI C'CwwG_G
Cuts at: 107 1417 1771 107
Size: 1310 354 2619
Fragments arranged by size:
2619 1310 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 186 1866 1999 2458 2667 186
Size: 1680 133 459 209 1802
Fragments arranged by size:
1802 1680 459 209 133
TatI w'GTAC_w
Cuts at: 3798 3798
Size: 4283
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3800 3832 3800
Size: 32 4251
Tth111I GACn'n_nGTC
Cuts at: 2080 2080
Size: 4283
XbaI T'CTAG_A
Cuts at: 1314 1314
Size: 4283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1778 1778
Size: 4283
XmaI C'CCGG_G
Cuts at: 1303 1303
Size: 4283
XmnI GAAnn'nnTTC
Cuts at: 268 268
Size: 4283
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BfrBI BglI BmgBI BmtI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI XhoI ZraI