(Circular) (Six-base) MAPSORT of: pF1KE2499.seq Check: 9165 from: 1 to: 16835 pF1KE2499 16835 bp With 182 enzymes: SgfI * July 3, 2020 20:21 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2628 4683 5943 6507 6726 7002 8034 10845 13504 Size: 2055 1260 564 219 276 1032 2811 2659 Cuts at: 13504 2628 Size: 5959 Fragments arranged by size: 5959 2811 2659 2055 1260 1032 564 276 219 AatII G_ACGT'C Cuts at: 8900 12580 8900 Size: 3680 13155 AccI GT'mk_AC Cuts at: 8873 11273 13873 8873 Size: 2400 2600 11835 Fragments arranged by size: 11835 2600 2400 Acc65I G'GTAC_C Cuts at: 710 13851 710 Size: 13141 3694 AclI AA'CG_TT Cuts at: 16675 16675 Size: 16835 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 164 1048 1174 1354 1684 2209 2362 2590 2959 Size: 884 126 180 330 525 153 228 369 Cuts at: 2959 3604 5998 6559 6844 8317 9340 9568 10086 Size: 645 2394 561 285 1473 1023 228 518 Cuts at: 10086 10653 12157 12313 12571 13330 14660 15092 15839 Size: 567 1504 156 258 759 1330 432 747 Cuts at: 15839 16107 164 Size: 268 892 Fragments arranged by size: 2394 1504 1473 1330 1023 892 884 759 747 645 567 561 525 518 432 369 330 285 268 258 228 228 180 156 153 126 AfeI AGC'GCT Cuts at: 3139 3715 3748 4681 4969 5086 6979 7326 7402 Size: 576 33 933 288 117 1893 347 76 Cuts at: 7402 7708 8797 9202 13174 13345 3139 Size: 306 1089 405 3972 171 6629 Fragments arranged by size: 6629 3972 1893 1089 933 576 405 347 306 288 171 117 76 33 AflIII A'CryG_T Cuts at: 4986 14187 15291 16268 4986 Size: 9201 1104 977 5553 Fragments arranged by size: 9201 5553 1104 977 AgeI A'CCGG_T Cuts at: 12106 13165 15223 12106 Size: 1059 2058 13718 Fragments arranged by size: 13718 2058 1059 AhdI GACnn_n'nnGTC Cuts at: 933 11697 933 Size: 10764 6071 AleI CACnn'nnGTG Cuts at: 377 2660 3240 8221 9073 10855 377 Size: 2283 580 4981 852 1782 6357 Fragments arranged by size: 6357 4981 2283 1782 852 580 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 4546 4922 8066 8617 9145 10594 12328 13585 4546 Size: 376 3144 551 528 1449 1734 1257 7796 Fragments arranged by size: 7796 3144 1734 1449 1257 551 528 376 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1090 1091 1122 1123 1090 Size: 1 31 1 16802 Fragments arranged by size: 16802 31 1 1 AlwNI CAG_nnn'CTG Cuts at: 142 720 1152 1770 2409 2823 3090 3093 3102 Size: 578 432 618 639 414 267 3 9 Cuts at: 3102 3948 4461 4620 4800 5313 5382 5445 5553 Size: 846 513 159 180 513 69 63 108 Cuts at: 5553 6393 6720 6750 6822 7257 7488 7641 7659 Size: 840 327 30 72 435 231 153 18 Cuts at: 7659 7776 7830 7914 7968 7989 8160 8349 8790 Size: 117 54 84 54 21 171 189 441 Cuts at: 8790 9186 9300 9546 9579 9996 10253 10407 10467 Size: 396 114 246 33 417 257 154 60 Cuts at: 10467 10767 11097 11298 13119 13308 13365 13461 14140 Size: 300 330 201 1821 189 57 96 679 Cuts at: 14140 15707 142 Size: 1567 1270 Fragments arranged by size: 1821 1567 1270 846 840 679 639 618 578 513 513 441 435 432 417 414 396 330 327 300 267 257 246 231 201 189 189 180 171 159 154 153 117 114 108 96 84 72 69 63 60 57 54 54 33 30 21 18 9 3 ApaI G_GGCC'C Cuts at: 2686 3510 3600 4791 8470 9454 9699 9846 10447 Size: 824 90 1191 3679 984 245 147 601 Cuts at: 10447 10995 11453 12181 13024 13813 2686 Size: 548 458 728 843 789 5708 Fragments arranged by size: 5708 3679 1191 984 843 824 789 728 601 548 458 245 147 90 ApaLI G'TGCA_C Cuts at: 2653 3113 3199 4625 8644 10621 15605 2653 Size: 460 86 1426 4019 1977 4984 3883 Fragments arranged by size: 4984 4019 3883 1977 1426 460 86 ApoI r'AATT_y Cuts at: 1888 6703 13839 16188 16444 1888 Size: 4815 7136 2349 256 2279 Fragments arranged by size: 7136 4815 2349 2279 256 AseI AT'TA_AT Cuts at: 20 20 Size: 16835 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 16835 AvaI C'yCGr_G Cuts at: 438 455 1732 1946 3031 3562 3793 6476 6569 Size: 17 1277 214 1085 531 231 2683 93 Cuts at: 6569 8470 8812 8891 9454 9566 9665 9839 10447 Size: 1901 342 79 563 112 99 174 608 Cuts at: 10447 10509 10789 10978 11167 11218 11452 12181 12625 Size: 62 280 189 189 51 234 729 444 Cuts at: 12625 12897 13679 13761 13785 13855 438 Size: 272 782 82 24 70 3418 Fragments arranged by size: 3418 2683 1901 1277 1085 782 729 608 563 531 444 342 280 272 234 231 214 189 189 174 112 99 93 82 79 70 62 51 24 17 BamHI G'GATC_C Cuts at: 12940 13860 12940 Size: 920 15915 BanI G'GyrC_C Cuts at: 521 710 1257 1577 1727 1951 2760 2837 3847 Size: 189 547 320 150 224 809 77 1010 Cuts at: 3847 4838 5296 6134 6254 9129 9253 9376 9705 Size: 991 458 838 120 2875 124 123 329 Cuts at: 9705 9782 11194 11777 11794 11980 12788 13474 13851 Size: 77 1412 583 17 186 808 686 377 Cuts at: 13851 14265 14513 14548 521 Size: 414 248 35 2808 Fragments arranged by size: 2875 2808 1412 1010 991 838 809 808 686 583 547 458 414 377 329 320 248 224 189 186 150 124 123 120 77 77 35 17 BanII G_rGCy'C Cuts at: 2213 2686 3155 3510 3563 3600 3794 4474 4791 Size: 473 469 355 53 37 194 680 317 Cuts at: 4791 6576 6878 7374 7454 8234 8470 9176 9246 Size: 1785 302 496 80 780 236 706 70 Cuts at: 9246 9454 9699 9773 9846 9860 9956 10139 10167 Size: 208 245 74 73 14 96 183 28 Cuts at: 10167 10225 10447 10995 11260 11453 11708 12086 12181 Size: 58 222 548 265 193 255 378 95 Cuts at: 12181 13024 13388 13813 13849 14879 2213 Size: 843 364 425 36 1030 4169 Fragments arranged by size: 4169 1785 1030 843 780 706 680 548 496 473 469 425 378 364 355 317 302 265 255 245 236 222 208 194 193 183 96 95 80 74 73 70 58 53 37 36 28 14 BbeI G_GCGC'C Cuts at: 1731 3851 4842 6138 6258 12792 13478 14269 14517 Size: 2120 991 1296 120 6534 686 791 248 Cuts at: 14517 1731 Size: 4049 Fragments arranged by size: 6534 4049 2120 1296 991 791 686 248 120 BbsI GAAGACnn'nnnn_ Cuts at: 398 1360 4957 5660 8252 11077 11994 12580 398 Size: 962 3597 703 2592 2825 917 586 4653 Fragments arranged by size: 4653 3597 2825 2592 962 917 703 586 BbvCI CC'TCA_GC Cuts at: 125 920 5510 5938 6146 6458 7169 7328 7385 Size: 795 4590 428 208 312 711 159 57 Cuts at: 7385 7952 10156 10835 11135 11752 125 Size: 567 2204 679 300 617 5208 Fragments arranged by size: 5208 4590 2204 795 711 679 617 567 428 312 300 208 159 57 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 2866 2900 3288 3322 4347 4381 7434 7468 8262 Size: 34 388 34 1025 34 3053 34 794 Cuts at: 8262 8296 9144 9178 2866 Size: 34 848 34 10523 Fragments arranged by size: 10523 3053 1025 848 794 388 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 14733 15494 16835 14733 Size: 761 1341 14733 Fragments arranged by size: 14733 1341 761 BclI T'GATC_A Cuts at: 11042 12767 14355 11042 Size: 1725 1588 13522 Fragments arranged by size: 13522 1725 1588 BglI GCCn_nnn'nGGC Cuts at: 1186 1865 2548 2788 3544 3574 4084 4504 4592 Size: 679 683 240 756 30 510 420 88 Cuts at: 4592 4768 4795 5053 5107 5458 5500 5593 6310 Size: 176 27 258 54 351 42 93 717 Cuts at: 6310 6451 6841 7054 8014 9449 9487 9703 10262 Size: 141 390 213 960 1435 38 216 559 Cuts at: 10262 10339 10429 10480 10931 11344 11599 13249 13303 Size: 77 90 51 451 413 255 1650 54 Cuts at: 13303 1186 Size: 4718 Fragments arranged by size: 4718 1650 1435 960 756 717 683 679 559 510 451 420 413 390 351 258 255 240 216 213 176 141 93 90 88 77 54 54 51 42 38 30 27 BglII A'GATC_T Cuts at: 623 8543 13517 14350 16387 623 Size: 7920 4974 833 2037 1071 Fragments arranged by size: 7920 4974 2037 1071 833 BlpI GC'TnA_GC Cuts at: 275 2713 3050 4241 4517 5018 6907 7334 7547 Size: 2438 337 1191 276 501 1889 427 213 Cuts at: 7547 9808 11480 13947 275 Size: 2261 1672 2467 3163 Fragments arranged by size: 3163 2467 2438 2261 1889 1672 1191 501 427 337 276 213 Bme1580I G_kGCm'C Cuts at: 1262 1914 2657 2686 2744 2765 3117 3203 3249 Size: 652 743 29 58 21 352 86 46 Cuts at: 3249 3510 3600 3657 4629 4791 5031 5613 6639 Size: 261 90 57 972 162 240 582 1026 Cuts at: 6639 8470 8648 8663 9386 9454 9647 9699 9846 Size: 1831 178 15 723 68 193 52 147 Cuts at: 9846 10119 10447 10625 10995 11197 11453 12181 13024 Size: 273 328 178 370 202 256 728 843 Cuts at: 13024 13409 13564 13813 14460 14553 15609 1262 Size: 385 155 249 647 93 1056 2488 Fragments arranged by size: 2488 1831 1056 1026 972 843 743 728 723 652 647 582 385 370 352 328 273 261 256 249 240 202 193 178 178 162 155 147 93 90 86 68 58 57 52 46 29 21 15 BmgBI CAC'GTC Cuts at: 909 990 1554 2895 3702 3777 8250 8670 10647 Size: 81 564 1341 807 75 4473 420 1977 Cuts at: 10647 13470 909 Size: 2823 4274 Fragments arranged by size: 4473 4274 2823 1977 1341 807 564 420 81 75 BmrI ACTGGGnnnn_n' Cuts at: 3811 10161 12233 14047 14128 14463 16607 3811 Size: 6350 2072 1814 81 335 2144 4039 Fragments arranged by size: 6350 4039 2144 2072 1814 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 453 2178 3081 4758 5541 6897 9879 10239 15202 Size: 1725 903 1677 783 1356 2982 360 4963 Cuts at: 15202 453 Size: 2086 Fragments arranged by size: 4963 2982 2086 1725 1677 1356 903 783 360 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 448 726 768 856 1098 1246 1342 1381 1759 Size: 278 42 88 242 148 96 39 378 Cuts at: 1759 2053 2139 2203 2488 2824 2995 3196 3409 Size: 294 86 64 285 336 171 201 213 Cuts at: 3409 3430 3463 3973 4024 4315 4822 4909 4984 Size: 21 33 510 51 291 507 87 75 Cuts at: 4984 5101 5290 5404 5575 5848 5926 6040 6046 Size: 117 189 114 171 273 78 114 6 Cuts at: 6046 6109 6232 6793 7312 7336 7510 7573 7756 Size: 63 123 561 519 24 174 63 183 Cuts at: 7756 7936 8011 8398 8626 8812 9169 9301 9757 Size: 180 75 387 228 186 357 132 456 Cuts at: 9757 10294 10375 10603 10789 11380 11668 12109 12337 Size: 537 81 228 186 591 288 441 228 Cuts at: 12337 12715 12960 13075 13144 13372 13600 448 Size: 378 245 115 69 228 228 3683 Fragments arranged by size: 3683 591 561 537 519 510 507 456 441 387 378 378 357 336 294 291 288 285 278 273 245 242 228 228 228 228 228 213 201 189 186 186 183 180 174 171 171 148 132 123 117 115 114 114 96 88 87 86 81 78 75 75 69 64 63 63 51 42 39 33 24 21 6 Bpu10I CC'TnA_GC Cuts at: 125 920 3502 5510 5938 6146 6458 6575 7169 Size: 795 2582 2008 428 208 312 117 594 Cuts at: 7169 7328 7385 7952 10156 10835 11135 11752 11770 Size: 159 57 567 2204 679 300 617 18 Cuts at: 11770 125 Size: 5190 Fragments arranged by size: 5190 2582 2204 2008 795 679 617 594 567 428 312 300 208 159 117 57 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1237 1549 3436 3508 3763 4087 5080 5716 5740 Size: 312 1887 72 255 324 993 636 24 Cuts at: 5740 6901 7108 7147 7327 7585 10165 11302 13498 Size: 1161 207 39 180 258 2580 1137 2196 Cuts at: 13498 14011 14890 15382 15680 1237 Size: 513 879 492 298 2392 Fragments arranged by size: 2580 2392 2196 1887 1161 1137 993 879 636 513 492 324 312 298 258 255 207 180 72 39 24 BsaI GGTCTCn'nnnn_ Cuts at: 1148 6772 9679 9877 10069 10493 10840 16539 1148 Size: 5624 2907 198 192 424 347 5699 1444 Fragments arranged by size: 5699 5624 2907 1444 424 347 198 192 BsaAI yAC'GTr Cuts at: 1311 2850 4137 4989 8777 10754 11115 11727 14188 Size: 1539 1287 852 3788 1977 361 612 2461 Cuts at: 14188 1311 Size: 3958 Fragments arranged by size: 3958 3788 2461 1977 1539 1287 852 612 361 BsaBI GATnn'nnATC Cuts at: 6 1132 9064 11047 12733 14349 16386 6 Size: 1126 7932 1983 1686 1616 2037 455 Fragments arranged by size: 7932 2037 1983 1686 1616 1126 455 BsaHI Gr'CG_yC Cuts at: 968 1436 1728 3629 3848 3863 4839 6135 6255 Size: 468 292 1901 219 15 976 1296 120 Cuts at: 6255 7394 8897 9593 9719 11825 12011 12577 12789 Size: 1139 1503 696 126 2106 186 566 212 Cuts at: 12789 13190 13442 13475 13709 14266 14514 15216 16712 Size: 401 252 33 234 557 248 702 1496 Cuts at: 16712 968 Size: 1091 Fragments arranged by size: 2106 1901 1503 1496 1296 1139 1091 976 702 696 566 557 468 401 292 252 248 234 219 212 186 126 120 33 15 BsaWI w'CCGG_w Cuts at: 7406 12106 13085 13165 14024 14545 15223 15497 15644 Size: 4700 979 80 859 521 678 274 147 Cuts at: 15644 16055 7406 Size: 411 8186 Fragments arranged by size: 8186 4700 979 859 678 521 411 274 147 80 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 403 433 459 489 724 754 1279 1309 2062 Size: 30 26 30 235 30 525 30 753 Cuts at: 2062 2092 5314 5344 6265 6295 8866 8896 10081 Size: 30 3222 30 921 30 2571 30 1185 Cuts at: 10081 10111 10279 10309 10918 10948 11193 11223 12703 Size: 30 168 30 609 30 245 30 1480 Cuts at: 12703 12733 12766 12796 13048 13078 13345 13375 403 Size: 30 33 30 252 30 267 30 3863 Fragments arranged by size: 3863 3222 2571 1480 1185 921 753 609 525 267 252 245 235 168 33 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 26 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1138 1429 1801 1998 2110 2113 2116 2962 3508 Size: 291 372 197 112 3 3 846 546 Cuts at: 3508 3529 3970 4000 4237 4240 4243 4273 4399 Size: 21 441 30 237 3 3 30 126 Cuts at: 4399 4417 4462 4555 4558 4651 4684 4819 4840 Size: 18 45 93 3 93 33 135 21 Cuts at: 4840 5026 5077 5149 5203 5242 5401 5404 5527 Size: 186 51 72 54 39 159 3 123 Cuts at: 5527 5530 5605 5845 5878 5974 5977 6136 6139 Size: 3 75 240 33 96 3 159 3 Cuts at: 6139 6163 6193 6196 6259 6475 6484 6568 6790 Size: 24 30 3 63 216 9 84 222 Cuts at: 6790 6823 6883 6886 6916 7006 7036 7096 7279 Size: 33 60 3 30 90 30 60 183 Cuts at: 7279 7402 7435 7624 7798 7861 7891 7915 7954 Size: 123 33 189 174 63 30 24 39 Cuts at: 7954 7969 8008 8041 8203 8713 9094 9298 9697 Size: 15 39 33 162 510 381 204 399 Cuts at: 9697 9868 10081 10690 10882 10885 10996 11584 11887 Size: 171 213 609 192 3 111 588 303 Cuts at: 11887 11959 12166 12370 12424 12490 12729 12732 12739 Size: 72 207 204 54 66 239 3 7 Cuts at: 12739 12921 12936 12939 13027 13570 13746 13749 1138 Size: 182 15 3 88 543 176 3 4224 Fragments arranged by size: 4224 846 609 588 546 543 510 441 399 381 372 303 291 240 239 237 222 216 213 207 204 204 197 192 189 186 183 182 176 174 171 162 159 159 135 126 123 123 112 111 96 93 93 90 88 84 75 72 72 66 63 63 60 60 54 54 51 45 39 39 39 33 33 33 33 33 30 30 30 30 30 30 24 24 21 21 18 15 15 9 7 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 175 244 270 1834 1851 2221 2583 2810 3248 Size: 69 26 1564 17 370 362 227 438 Cuts at: 3248 3294 3399 3734 5876 6030 7397 7769 8725 Size: 46 105 335 2142 154 1367 372 956 Cuts at: 8725 9596 9625 9739 10218 10702 10994 11594 11707 Size: 871 29 114 479 484 292 600 113 Cuts at: 11707 12983 13159 14260 15018 15595 16091 175 Size: 1276 176 1101 758 577 496 919 Fragments arranged by size: 2142 1564 1367 1276 1101 956 919 871 758 600 577 496 484 479 438 372 370 362 335 292 227 176 154 114 113 105 69 46 29 26 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 337 598 1012 1291 1576 1603 1741 1855 2584 Size: 261 414 279 285 27 138 114 729 Cuts at: 2584 2672 2917 2950 2965 3854 4717 4928 6178 Size: 88 245 33 15 889 863 211 1250 Cuts at: 6178 6382 6502 6913 7165 7423 7501 7645 8461 Size: 204 120 411 252 258 78 144 816 Cuts at: 8461 11443 13122 13378 337 Size: 2982 1679 256 3794 Fragments arranged by size: 3794 2982 1679 1250 889 863 816 729 414 411 285 279 261 258 256 252 245 211 204 144 138 120 114 88 78 33 27 15 BsiEI CG_ry'CG Cuts at: 104 759 1088 2127 5571 6002 6176 8498 9168 Size: 655 329 1039 3444 431 174 2322 670 Cuts at: 9168 11022 11466 12209 12777 12834 13298 13773 14377 Size: 1854 444 743 568 57 464 475 604 Cuts at: 14377 14423 15631 104 Size: 46 1208 1308 Fragments arranged by size: 3444 2322 1854 1308 1208 1039 743 670 655 604 568 475 464 444 431 329 174 57 46 BsiHKAI G_wGCw'C Cuts at: 2213 2657 2714 3117 3155 3203 3503 3563 3794 Size: 444 57 403 38 48 300 60 231 Cuts at: 3794 4474 4629 6878 7454 8120 8234 8591 8648 Size: 680 155 2249 576 666 114 357 57 Cuts at: 8648 9176 9773 9860 9956 10139 10625 12086 13849 Size: 528 597 87 96 183 486 1461 1763 Cuts at: 13849 14627 14817 15609 2213 Size: 778 190 792 3439 Fragments arranged by size: 3439 2249 1763 1461 792 778 680 666 597 576 528 486 444 403 357 300 231 190 183 155 114 96 87 60 57 57 48 38 BsiWI C'GTAC_G Cuts at: 4147 4147 Size: 16835 BsmI GAATG_Cn' Cuts at: 4439 4439 Size: 16835 BsmBI CGTCTCn'nnnn_ Cuts at: 746 957 3181 4885 5344 7261 7642 8809 9206 Size: 211 2224 1704 459 1917 381 1167 397 Cuts at: 9206 9534 10786 12622 12978 13009 13042 746 Size: 328 1252 1836 356 31 33 4539 Fragments arranged by size: 4539 2224 1917 1836 1704 1252 1167 459 397 381 356 328 211 33 31 Bsp1286I G_dGCh'C Cuts at: 1262 1914 2213 2657 2686 2714 2744 2765 3117 Size: 652 299 444 29 28 30 21 352 Cuts at: 3117 3155 3203 3249 3503 3510 3563 3600 3657 Size: 38 48 46 254 7 53 37 57 Cuts at: 3657 3794 4474 4629 4791 5031 5613 6576 6639 Size: 137 680 155 162 240 582 963 63 Cuts at: 6639 6878 7374 7454 8120 8234 8470 8591 8648 Size: 239 496 80 666 114 236 121 57 Cuts at: 8648 8663 9176 9246 9386 9454 9647 9699 9773 Size: 15 513 70 140 68 193 52 74 Cuts at: 9773 9846 9860 9956 10119 10139 10167 10225 10447 Size: 73 14 96 163 20 28 58 222 Cuts at: 10447 10625 10995 11197 11260 11453 11708 12086 12181 Size: 178 370 202 63 193 255 378 95 Cuts at: 12181 13024 13388 13409 13564 13813 13849 14460 14553 Size: 843 364 21 155 249 36 611 93 Cuts at: 14553 14627 14817 14879 15609 1262 Size: 74 190 62 730 2488 Fragments arranged by size: 2488 963 843 730 680 666 652 611 582 513 496 444 378 370 364 352 299 255 254 249 240 239 236 222 202 193 193 190 178 163 162 155 155 140 137 121 114 96 95 93 80 74 74 73 70 68 63 63 62 58 57 57 53 52 48 46 38 37 36 30 29 28 28 21 21 20 15 14 7 BspEI T'CCGG_A Cuts at: 7406 16055 7406 Size: 8649 8186 BspHI T'CATG_A Cuts at: 9462 16011 9462 Size: 6549 10286 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1094 1769 1778 1838 1991 2483 2628 3405 4151 Size: 675 9 60 153 492 145 777 746 Cuts at: 4151 4683 4863 5943 6507 6726 6806 7002 8034 Size: 532 180 1080 564 219 80 196 1032 Cuts at: 8034 8270 8789 9017 9338 10845 11189 11285 11880 Size: 236 519 228 321 1507 344 96 595 Cuts at: 11880 12500 13504 13885 14099 14401 14782 1094 Size: 620 1004 381 214 302 381 3147 Fragments arranged by size: 3147 1507 1080 1032 1004 777 746 675 620 595 564 532 519 492 381 381 344 321 302 236 228 219 214 196 180 153 145 96 80 60 9 BsrBI CCG'CTC Cuts at: 50 279 306 870 1234 1326 1908 2909 3696 Size: 229 27 564 364 92 582 1001 787 Cuts at: 3696 3726 4269 4350 4836 5022 5052 5073 5685 Size: 30 543 81 486 186 30 21 612 Cuts at: 5685 5910 6129 6225 6271 6534 7302 9822 10773 Size: 225 219 96 46 263 768 2520 951 Cuts at: 10773 11409 11484 12570 12782 12836 12920 15127 15181 Size: 636 75 1086 212 54 84 2207 54 Cuts at: 15181 16665 16830 50 Size: 1484 165 55 Fragments arranged by size: 2520 2207 1484 1086 1001 951 787 768 636 612 582 564 543 486 364 263 229 225 219 212 186 165 96 92 84 81 75 55 54 54 46 30 30 27 21 BsrDI GCAATG_nn' Cuts at: 14747 14747 Size: 16835 BsrFI r'CCGG_y Cuts at: 224 782 821 2228 3038 3231 3543 4071 4526 Size: 558 39 1407 810 193 312 528 455 Cuts at: 4526 5566 5570 8170 8423 8678 9098 9373 9753 Size: 1040 4 2600 253 255 420 275 380 Cuts at: 9753 9785 10261 10489 11017 11494 11605 11797 12106 Size: 32 476 228 528 477 111 192 309 Cuts at: 12106 12365 12859 12907 13165 13336 13369 13660 13672 Size: 259 494 48 258 171 33 291 12 Cuts at: 13672 13684 13714 14833 15014 15223 224 Size: 12 30 1119 181 209 1836 Fragments arranged by size: 2600 1836 1407 1119 1040 810 558 528 528 494 477 476 455 420 380 312 309 291 275 259 258 255 253 228 209 193 192 181 171 111 48 39 33 32 30 12 12 4 BsrGI T'GTAC_A Cuts at: 10859 10859 Size: 16835 BssHII G'CGCG_C Cuts at: 4771 5056 8502 12213 4771 Size: 285 3446 3711 9393 Fragments arranged by size: 9393 3711 3446 285 BssSI C'ACGA_G Cuts at: 1621 3511 8764 10417 10456 11443 15106 15464 1621 Size: 1890 5253 1653 39 987 3663 358 2992 Fragments arranged by size: 5253 3663 2992 1890 1653 987 358 39 BstAPI GCAn_nnn'nTGC Cuts at: 1719 1770 2409 2844 3093 3207 3759 3948 4377 Size: 51 639 435 249 114 552 189 429 Cuts at: 4377 4461 4620 4624 6393 6411 6720 7143 7623 Size: 84 159 4 1769 18 309 423 480 Cuts at: 7623 7641 7659 9436 9579 10253 11987 13119 14212 Size: 18 18 1777 143 674 1734 1132 1093 Cuts at: 14212 1719 Size: 4342 Fragments arranged by size: 4342 1777 1769 1734 1132 1093 674 639 552 480 435 429 423 309 249 189 159 143 114 84 51 18 18 18 4 BstBI TT'CG_AA Cuts at: 15196 15196 Size: 16835 BstEII G'GTnAC_C Cuts at: 7470 8952 10023 11025 11490 11949 7470 Size: 1482 1071 1002 465 459 12356 Fragments arranged by size: 12356 1482 1071 1002 465 459 BstXI CCAn_nnnn'nTGG Cuts at: 643 2221 3441 3465 3900 643 Size: 1578 1220 24 435 13578 Fragments arranged by size: 13578 1578 1220 435 24 BstYI r'GATC_y Cuts at: 1 623 1133 3884 4484 4547 5966 7493 8543 Size: 622 510 2751 600 63 1419 1527 1050 Cuts at: 8543 12422 12940 13517 13860 14327 14350 14685 14931 Size: 3879 518 577 343 467 23 335 246 Cuts at: 14931 15943 16029 16041 16387 1 Size: 1012 86 12 346 449 Fragments arranged by size: 3879 2751 1527 1419 1050 1012 622 600 577 518 510 467 449 346 343 335 246 86 63 23 12 Bsu36I CC'TnA_GG Cuts at: 902 2023 8394 12353 902 Size: 1121 6371 3959 5384 Fragments arranged by size: 6371 5384 3959 1121 BtgI C'CryG_G Cuts at: 2261 3474 3969 5318 6836 8096 8219 8621 8663 Size: 1213 495 1349 1518 1260 123 402 42 Cuts at: 8663 9350 9419 9605 9914 10028 10598 10926 11261 Size: 687 69 186 309 114 570 328 335 Cuts at: 11261 11844 11867 11906 12300 12332 12374 13025 13695 Size: 583 23 39 394 32 42 651 670 Cuts at: 13695 13823 2261 Size: 128 5273 Fragments arranged by size: 5273 1518 1349 1260 1213 687 670 651 583 570 495 402 394 335 328 309 186 128 123 114 69 42 42 39 32 23 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 279 416 546 738 3240 3341 7527 9249 11976 Size: 137 130 192 2502 101 4186 1722 2727 Cuts at: 11976 12798 14785 14966 15129 279 Size: 822 1987 181 163 1985 Fragments arranged by size: 4186 2727 2502 1987 1985 1722 822 192 181 163 137 130 101 BtsI GCAGTG_nn' Cuts at: 171 190 2302 2419 4618 4911 6757 8033 9437 Size: 19 2112 117 2199 293 1846 1276 1404 Cuts at: 9437 9974 10254 14078 171 Size: 537 280 3824 2928 Fragments arranged by size: 3824 2928 2199 2112 1846 1404 1276 537 293 280 117 19 ClaI AT'CG_AT Cuts at: 12803 12803 Size: 16835 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 1676 1711 12891 12926 1676 Size: 35 11180 35 5585 Fragments arranged by size: 11180 5585 35 35 DraI TTT'AAA Cuts at: 13834 13834 Size: 16835 DraIII CAC_nnn'GTG Cuts at: 338 1803 1918 2834 4624 8658 9642 10635 338 Size: 1465 115 916 1790 4034 984 993 6538 Fragments arranged by size: 6538 4034 1790 1465 993 984 916 115 DrdI GACnn_nn'nnGTC Cuts at: 127 913 12754 13290 14541 15399 127 Size: 786 11841 536 1251 858 1563 Fragments arranged by size: 11841 1563 1251 858 786 536 EaeI y'GGCC_r Cuts at: 405 665 756 1085 1313 2540 2618 2921 3545 Size: 260 91 329 228 1227 78 303 624 Cuts at: 3545 3902 4085 4274 4313 5315 5459 5492 5568 Size: 357 183 189 39 1002 144 33 76 Cuts at: 5568 5585 5645 5705 5999 6173 7355 7760 7835 Size: 17 60 60 294 174 1182 405 75 Cuts at: 7835 8073 8495 8535 9347 9755 10367 11019 11600 Size: 238 422 40 812 408 612 652 581 Cuts at: 11600 11799 12101 12206 13295 13338 13770 13826 14420 Size: 199 302 105 1089 43 432 56 594 Cuts at: 14420 14594 14985 15012 16357 405 Size: 174 391 27 1345 883 Fragments arranged by size: 1345 1227 1182 1089 1002 883 812 652 624 612 594 581 432 422 408 405 391 357 329 303 302 294 260 238 228 199 189 183 174 174 144 105 91 78 76 75 60 60 56 43 40 39 33 27 17 EagI C'GGCC_G Cuts at: 756 1085 5568 5999 6173 8495 11019 12206 13295 Size: 329 4483 431 174 2322 2524 1187 1089 Cuts at: 13295 13770 14420 756 Size: 475 650 3171 Fragments arranged by size: 4483 3171 2524 2322 1187 1089 650 475 431 329 174 EarI CTCTTCn'nnn_ Cuts at: 769 1549 2854 3116 5146 5615 6157 6332 6518 Size: 780 1305 262 2030 469 542 175 186 Cuts at: 6518 6883 7750 8557 10534 10886 11539 12268 12406 Size: 365 867 807 1977 352 653 729 138 Cuts at: 12406 13746 14858 15068 769 Size: 1340 1112 210 2536 Fragments arranged by size: 2536 2030 1977 1340 1305 1112 867 807 780 729 653 542 469 365 352 262 210 186 175 138 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 880 1537 2439 4360 4495 4648 4657 4741 4837 Size: 657 902 1921 135 153 9 84 96 Cuts at: 4837 4846 4930 5146 5209 5239 5305 5632 5695 Size: 9 84 216 63 30 66 327 63 Cuts at: 5695 5758 5785 5812 6028 6133 6349 6451 6481 Size: 63 27 27 216 105 216 102 30 Cuts at: 6481 6502 6658 6844 7033 7189 7276 7432 8154 Size: 21 156 186 189 156 87 156 722 Cuts at: 8154 8587 8734 10564 10711 10840 11074 11220 11239 Size: 433 147 1830 147 129 234 146 19 Cuts at: 11239 12052 12298 15043 15353 15499 16645 880 Size: 813 246 2745 310 146 1146 1070 Fragments arranged by size: 2745 1921 1830 1146 1070 902 813 722 657 433 327 310 246 234 216 216 216 189 186 156 156 156 153 147 147 146 146 135 129 105 102 96 87 84 84 66 63 63 63 30 30 27 27 21 19 9 9 EcoICRI GAG'CTC Cuts at: 2211 3153 3561 3792 4472 6876 7452 8232 9174 Size: 942 408 231 680 2404 576 780 942 Cuts at: 9174 9771 9858 9954 10137 12084 13847 2211 Size: 597 87 96 183 1947 1763 5199 Fragments arranged by size: 5199 2404 1947 1763 942 942 780 680 597 576 408 231 183 96 87 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 164 448 726 768 856 1048 1098 1174 1246 Size: 284 278 42 88 192 50 76 72 Cuts at: 1246 1342 1354 1381 1684 1759 2053 2139 2203 Size: 96 12 27 303 75 294 86 64 Cuts at: 2203 2209 2362 2488 2590 2824 2959 2995 3196 Size: 6 153 126 102 234 135 36 201 Cuts at: 3196 3409 3430 3463 3604 3973 4024 4315 4822 Size: 213 21 33 141 369 51 291 507 Cuts at: 4822 4909 4984 5101 5290 5404 5575 5848 5926 Size: 87 75 117 189 114 171 273 78 Cuts at: 5926 5998 6040 6046 6109 6232 6559 6793 6844 Size: 72 42 6 63 123 327 234 51 Cuts at: 6844 7312 7336 7510 7573 7756 7936 8011 8317 Size: 468 24 174 63 183 180 75 306 Cuts at: 8317 8398 8626 8812 9169 9301 9340 9568 9757 Size: 81 228 186 357 132 39 228 189 Cuts at: 9757 10086 10294 10375 10603 10653 10789 11380 11668 Size: 329 208 81 228 50 136 591 288 Cuts at: 11668 12109 12157 12313 12337 12571 12715 12960 13075 Size: 441 48 156 24 234 144 245 115 Cuts at: 13075 13144 13330 13372 13600 14660 15092 15839 16107 Size: 69 186 42 228 1060 432 747 268 Cuts at: 16107 164 Size: 892 Fragments arranged by size: 1060 892 747 591 507 468 441 432 369 357 329 327 306 303 294 291 288 284 278 273 268 245 234 234 234 228 228 228 228 213 208 201 192 189 189 186 186 183 180 174 171 156 153 144 141 136 135 132 126 123 117 115 114 102 96 88 87 86 81 81 78 76 75 75 75 72 72 69 64 63 63 51 51 50 50 48 42 42 42 39 36 33 27 24 24 21 12 6 6 EcoNI CCTnn'n_nnAGG Cuts at: 358 427 847 2479 3106 3952 4846 4978 5161 Size: 69 420 1632 627 846 894 132 183 Cuts at: 5161 6988 7534 8377 8605 9538 9550 9679 10084 Size: 1827 546 843 228 933 12 129 405 Cuts at: 10084 10354 10531 10582 10951 11146 11209 11677 12265 Size: 270 177 51 369 195 63 468 588 Cuts at: 12265 12316 13312 13414 358 Size: 51 996 102 3779 Fragments arranged by size: 3779 1827 1632 996 933 894 846 843 627 588 546 468 420 405 369 270 228 195 183 177 132 129 102 69 63 51 51 12 EcoO109I rG'GnC_Cy Cuts at: 432 717 873 1767 2027 2472 2683 2820 3506 Size: 285 156 894 260 445 211 137 686 Cuts at: 3506 3945 4788 4797 7086 7656 8467 8523 8571 Size: 439 843 9 2289 570 811 56 48 Cuts at: 8571 8808 9006 9114 9451 9507 9543 9555 9576 Size: 237 198 108 337 56 36 12 21 Cuts at: 9576 9695 9696 9909 10152 10206 10320 10444 10548 Size: 119 1 213 243 54 114 124 104 Cuts at: 10548 10785 11139 11439 11449 11574 11928 12178 12234 Size: 237 354 300 10 125 354 250 56 Cuts at: 12234 12282 12669 12980 13021 13203 13269 13305 13419 Size: 48 387 311 41 182 66 36 114 Cuts at: 13419 13790 13809 13810 13974 432 Size: 371 19 1 164 3293 Fragments arranged by size: 3293 2289 894 843 811 686 570 445 439 387 371 354 354 337 311 300 285 260 250 243 237 237 213 211 198 182 164 156 137 125 124 119 114 114 108 104 66 56 56 56 54 48 48 41 36 36 21 19 12 10 9 1 1 EcoRI G'AATT_C Cuts at: 1888 13839 1888 Size: 11951 4884 EcoRV GAT'ATC Cuts at: 633 633 Size: 16835 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 345 6003 8763 10740 11856 12474 14854 345 Size: 5658 2760 1977 1116 618 2380 2326 Fragments arranged by size: 5658 2760 2380 2326 1977 1116 618 FseI GG_CCGG'CC Cuts at: 5570 11021 13340 5570 Size: 5451 2319 9065 Fragments arranged by size: 9065 5451 2319 FspI TGC'GCA Cuts at: 4534 5884 6067 6604 7205 9121 11719 12598 14616 Size: 1350 183 537 601 1916 2598 879 2018 Cuts at: 14616 4534 Size: 6753 Fragments arranged by size: 6753 2598 2018 1916 1350 879 601 537 183 HaeII r_GCGC'y Cuts at: 245 1629 1731 1814 2170 2958 3141 3717 3750 Size: 1384 102 83 356 788 183 576 33 Cuts at: 3750 3851 3969 4587 4683 4842 4971 5088 5113 Size: 101 118 618 96 159 129 117 25 Cuts at: 5113 5313 5784 5871 5977 6138 6211 6258 6981 Size: 200 471 87 106 161 73 47 723 Cuts at: 6981 7328 7341 7404 7530 7710 8799 9204 9410 Size: 347 13 63 126 180 1089 405 206 Cuts at: 9410 10407 11751 11914 12606 12792 13176 13347 13478 Size: 997 1344 163 692 186 384 171 131 Cuts at: 13478 14269 14517 15539 16518 245 Size: 791 248 1022 979 562 Fragments arranged by size: 1384 1344 1089 1022 997 979 791 788 723 692 618 576 562 471 405 384 356 347 248 206 200 186 183 180 171 163 161 159 131 129 126 118 117 106 102 101 96 87 83 73 63 47 33 25 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 919 951 5503 5535 6265 6297 10081 10113 10279 Size: 32 4552 32 730 32 3784 32 166 Cuts at: 10279 10311 10492 10524 11148 11180 11212 11244 11683 Size: 32 181 32 624 32 32 32 439 Cuts at: 11683 11715 12226 12258 12599 12631 12766 12798 12880 Size: 32 511 32 341 32 135 32 82 Cuts at: 12880 12912 13048 13080 14677 14707 14709 14739 919 Size: 32 136 32 1597 30 2 30 3015 Fragments arranged by size: 4552 3784 3015 1597 730 624 511 439 341 181 166 136 135 82 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 345 8439 13197 13874 345 Size: 8094 4758 677 3306 Fragments arranged by size: 8094 4758 3306 677 HindIII A'AGCT_T Cuts at: 7668 7668 Size: 16835 KasI G'GCGC_C Cuts at: 1727 3847 4838 6134 6254 12788 13474 14265 14513 Size: 2120 991 1296 120 6534 686 791 248 Cuts at: 14513 1727 Size: 4049 Fragments arranged by size: 6534 4049 2120 1296 991 791 686 248 120 KpnI G_GTAC'C Cuts at: 714 13855 714 Size: 13141 3694 MluI A'CGCG_T Cuts at: 16268 16268 Size: 16835 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 632 1430 4550 7599 9341 9365 9569 10946 11801 Size: 798 3120 3049 1742 24 204 1377 855 Cuts at: 11801 15506 15690 632 Size: 3705 184 1777 Fragments arranged by size: 3705 3120 3049 1777 1742 1377 855 798 204 184 24 MscI TGG'CCA Cuts at: 407 1315 2542 2923 5461 5494 7762 7837 8537 Size: 908 1227 381 2538 33 2268 75 700 Cuts at: 8537 11602 14596 407 Size: 3065 2994 2646 Fragments arranged by size: 3065 2994 2646 2538 2268 1227 908 700 381 75 33 MslI CAynn'nnrTG Cuts at: 377 805 2137 2660 3240 8221 9070 9073 9349 Size: 428 1332 523 580 4981 849 3 276 Cuts at: 9349 10855 14824 14951 377 Size: 1506 3969 127 2261 Fragments arranged by size: 4981 3969 2261 1506 1332 849 580 523 428 276 127 3 MspA1I CmG'CkG Cuts at: 219 233 274 746 1023 1278 1422 1488 1851 Size: 14 41 472 277 255 144 66 363 Cuts at: 1851 2316 2508 2568 2676 3090 3174 3219 3476 Size: 465 192 60 108 414 84 45 257 Cuts at: 3476 3684 3738 3813 3900 3930 4044 4251 4305 Size: 208 54 75 87 30 114 207 54 Cuts at: 4305 4311 4461 4785 4962 5004 5247 5280 5286 Size: 6 150 324 177 42 243 33 6 Cuts at: 5286 5320 5373 5379 5637 5643 5670 5817 5880 Size: 34 53 6 258 6 27 147 63 Cuts at: 5880 5946 6004 6105 6111 6198 6294 6300 6423 Size: 66 58 101 6 87 96 6 123 Cuts at: 6423 6676 6771 6819 7074 7101 7257 7284 7308 Size: 253 95 48 255 27 156 27 24 Cuts at: 7308 7314 7638 7701 7776 7794 7827 7914 7926 Size: 6 324 63 75 18 33 87 12 Cuts at: 7926 7980 8098 8199 8346 9000 9186 9600 9780 Size: 54 118 101 147 654 186 414 180 Cuts at: 9780 9798 9987 10106 10464 10764 10989 10998 11598 Size: 18 189 119 358 300 225 9 600 Cuts at: 11598 11697 11766 11805 11919 11987 12498 12549 12987 Size: 99 69 39 114 68 511 51 438 Cuts at: 12987 13116 13631 13648 13697 13946 14260 14620 15633 Size: 129 515 17 49 249 314 360 1013 Cuts at: 15633 15878 219 Size: 245 1176 Fragments arranged by size: 1176 1013 654 600 515 511 472 465 438 414 414 363 360 358 324 324 314 300 277 258 257 255 255 253 249 245 243 225 208 207 192 189 186 180 177 156 150 147 147 144 129 123 119 118 114 114 108 101 101 99 96 95 87 87 87 84 75 75 69 68 66 66 63 63 60 58 54 54 54 53 51 49 48 45 42 41 39 34 33 33 30 27 27 27 24 18 18 17 14 12 9 6 6 6 6 6 6 6 NaeI GCC'GGC Cuts at: 226 3233 3545 4528 5568 8172 9100 9375 9755 Size: 3007 312 983 1040 2604 928 275 380 Cuts at: 9755 9787 10263 11019 12861 13338 13686 15016 226 Size: 32 476 756 1842 477 348 1330 2045 Fragments arranged by size: 3007 2604 2045 1842 1330 1040 983 928 756 477 476 380 348 312 275 32 NarI GG'CG_CC Cuts at: 1728 3848 4839 6135 6255 12789 13475 14266 14514 Size: 2120 991 1296 120 6534 686 791 248 Cuts at: 14514 1728 Size: 4049 Fragments arranged by size: 6534 4049 2120 1296 991 791 686 248 120 NcoI C'CATG_G Cuts at: 11867 12300 11867 Size: 433 16402 NgoMIV G'CCGG_C Cuts at: 224 3231 3543 4526 5566 8170 9098 9373 9753 Size: 3007 312 983 1040 2604 928 275 380 Cuts at: 9753 9785 10261 11017 12859 13336 13684 15014 224 Size: 32 476 756 1842 477 348 1330 2045 Fragments arranged by size: 3007 2604 2045 1842 1330 1040 983 928 756 477 476 380 348 312 275 32 NotI GC'GGCC_GC Cuts at: 5999 5999 Size: 16835 NspI r_CATG'y Cuts at: 4380 12894 13888 14388 15295 4380 Size: 8514 994 500 907 5920 Fragments arranged by size: 8514 5920 994 907 500 PasI CC'CwG_GG Cuts at: 181 13803 181 Size: 13622 3213 PciI A'CATG_T Cuts at: 15291 15291 Size: 16835 PflMI CCAn_nnn'nTGG Cuts at: 1224 1278 2679 2823 11001 1224 Size: 54 1401 144 8178 7058 Fragments arranged by size: 8178 7058 1401 144 54 PfoI T'CCnGG_A Cuts at: 263 893 1319 3554 4127 7748 7988 8582 11186 Size: 630 426 2235 573 3621 240 594 2604 Cuts at: 11186 11972 12086 12976 13037 13349 263 Size: 786 114 890 61 312 3749 Fragments arranged by size: 3749 3621 2604 2235 890 786 630 594 573 426 312 240 114 61 PmeI GTTT'AAAC Cuts at: 13834 13834 Size: 16835 PmlI CAC'GTG Cuts at: 1311 2850 4989 1311 Size: 1539 2139 13157 Fragments arranged by size: 13157 2139 1539 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 11128 11160 15974 16006 11128 Size: 32 4814 32 11957 Fragments arranged by size: 11957 4814 32 32 PpuMI rG'GwC_Cy Cuts at: 432 873 1767 2027 2472 7086 8523 8808 9006 Size: 441 894 260 445 4614 1437 285 198 Cuts at: 9006 9507 9909 10152 10206 10785 11139 12234 12980 Size: 501 402 243 54 579 354 1095 746 Cuts at: 12980 13269 13790 432 Size: 289 521 3477 Fragments arranged by size: 4614 3477 1437 1095 894 746 579 521 501 445 441 402 354 289 285 260 243 198 54 PshAI GACnn'nnGTC Cuts at: 10198 10198 Size: 16835 PspOMI G'GGCC_C Cuts at: 2682 3506 3596 4787 8466 9450 9695 9842 10443 Size: 824 90 1191 3679 984 245 147 601 Cuts at: 10443 10991 11449 12177 13020 13809 2682 Size: 548 458 728 843 789 5708 Fragments arranged by size: 5708 3679 1191 984 843 824 789 728 601 548 458 245 147 90 PspXI vC'TCGA_Gb Cuts at: 3562 3793 3562 Size: 231 16604 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 802 834 2341 2373 4177 4209 8761 8793 10738 Size: 32 1507 32 1804 32 4552 32 1945 Cuts at: 10738 10770 12472 12504 802 Size: 32 1702 32 5133 Fragments arranged by size: 5133 4552 1945 1804 1702 1507 32 32 32 32 32 32 PstI C_TGCA'G Cuts at: 491 1022 1394 1448 1473 1715 1766 1988 2291 Size: 531 372 54 25 242 51 222 303 Cuts at: 2291 2414 2483 3073 3098 3173 3680 3860 3935 Size: 123 69 590 25 75 507 180 75 Cuts at: 3935 4370 4457 4568 4616 4796 4820 4925 4979 Size: 435 87 111 48 180 24 105 54 Cuts at: 4979 5129 5408 5424 6299 6332 6398 6594 6770 Size: 150 279 16 875 33 66 196 176 Cuts at: 6770 6818 6989 7187 7229 7598 7646 7664 7910 Size: 48 171 198 42 369 48 18 246 Cuts at: 7910 7985 8165 8198 8267 8339 8786 9191 9875 Size: 75 180 33 69 72 447 405 684 Cuts at: 9875 10244 11918 12497 13427 13882 14567 491 Size: 369 1674 579 930 455 685 2759 Fragments arranged by size: 2759 1674 930 875 685 684 590 579 531 507 455 447 435 405 372 369 369 303 279 246 242 222 198 196 180 180 180 176 171 150 123 111 105 87 75 75 75 72 69 69 66 54 54 51 48 48 48 42 33 33 25 25 24 18 16 PvuI CG_AT'CG Cuts at: 104 104 Size: 16835 PvuII CAG'CTG Cuts at: 274 746 1023 1422 1851 2316 2508 2568 2676 Size: 472 277 399 429 465 192 60 108 Cuts at: 2676 3090 3174 3738 3813 3930 4044 4311 4461 Size: 414 84 564 75 117 114 267 150 Cuts at: 4461 4962 5004 5286 5379 5643 5880 6111 6294 Size: 501 42 282 93 264 237 231 183 Cuts at: 6294 6300 6423 6676 6771 7074 7257 7314 7638 Size: 6 123 253 95 303 183 57 324 Cuts at: 7638 7776 7914 7980 8346 9186 9600 9798 10464 Size: 138 138 66 366 840 414 198 666 Cuts at: 10464 10764 10998 11598 11697 11766 11805 11919 11987 Size: 300 234 600 99 69 39 114 68 Cuts at: 11987 12498 12987 14260 14620 274 Size: 511 489 1273 360 2489 Fragments arranged by size: 2489 1273 840 666 600 564 511 501 489 472 465 429 414 414 399 366 360 324 303 300 282 277 267 264 253 237 234 231 198 192 183 183 150 138 138 123 117 114 114 108 99 95 93 84 75 69 68 66 60 57 42 39 6 RsrII CG'GwC_CG Cuts at: 15030 15030 Size: 16835 SacI G_AGCT'C Cuts at: 2213 3155 3563 3794 4474 6878 7454 8234 9176 Size: 942 408 231 680 2404 576 780 942 Cuts at: 9176 9773 9860 9956 10139 12086 13849 2213 Size: 597 87 96 183 1947 1763 5199 Fragments arranged by size: 5199 2404 1947 1763 942 942 780 680 597 576 408 231 183 96 87 SacII CC_GC'GG Cuts at: 3477 5321 8099 13698 3477 Size: 1844 2778 5599 6614 Fragments arranged by size: 6614 5599 2778 1844 SalI G'TCGA_C Cuts at: 13872 13872 Size: 16835 SanDI GG'GwC_CC Cuts at: 9507 9507 Size: 16835 SapI GCTCTTCn'nnn_ Cuts at: 1549 2854 5146 6157 6883 7750 11539 14858 15068 Size: 1305 2292 1011 726 867 3789 3319 210 Cuts at: 15068 1549 Size: 3316 Fragments arranged by size: 3789 3319 3316 2292 1305 1011 867 726 210 SbfI CC_TGCA'GG Cuts at: 1766 4796 4979 6989 8267 13882 1766 Size: 3030 183 2010 1278 5615 4719 Fragments arranged by size: 5615 4719 3030 2010 1278 183 ScaI AGT'ACT Cuts at: 9970 16352 9970 Size: 6382 10453 SexAI A'CCwGG_T Cuts at: 13091 13091 Size: 16835 SfcI C'TryA_G Cuts at: 33 487 1018 1390 1444 1469 1711 1762 1984 Size: 454 531 372 54 25 242 51 222 Cuts at: 1984 2287 2311 2410 2479 3069 3094 3169 3676 Size: 303 24 99 69 590 25 75 507 Cuts at: 3676 3856 3931 4155 4366 4453 4564 4612 4792 Size: 180 75 224 211 87 111 48 180 Cuts at: 4792 4816 4921 4975 5125 5404 5420 6295 6328 Size: 24 105 54 150 279 16 875 33 Cuts at: 6328 6382 6394 6590 6766 6814 6985 7183 7225 Size: 54 12 196 176 48 171 198 42 Cuts at: 7225 7594 7642 7660 7696 7906 7981 8161 8194 Size: 369 48 18 36 210 75 180 33 Cuts at: 8194 8263 8335 8782 9187 9729 9871 10240 10494 Size: 69 72 447 405 542 142 369 254 Cuts at: 10494 11914 12493 13423 13611 13620 13878 14563 15556 Size: 1420 579 930 188 9 258 685 993 Cuts at: 15556 15747 33 Size: 191 1121 Fragments arranged by size: 1420 1121 993 930 875 685 590 579 542 531 507 454 447 405 372 369 369 303 279 258 254 242 224 222 211 210 198 196 191 188 180 180 180 176 171 150 142 111 105 99 87 75 75 75 72 69 69 54 54 54 51 48 48 48 42 36 33 33 25 25 24 24 18 16 12 9 SfiI GGCCn_nnn'nGGCC Cuts at: 2548 2788 3544 3574 4084 4795 5593 6310 6841 Size: 240 756 30 510 711 798 717 531 Cuts at: 6841 9449 13249 13303 2548 Size: 2608 3800 54 6080 Fragments arranged by size: 6080 3800 2608 798 756 717 711 531 510 240 54 30 SfoI GGC'GCC Cuts at: 1729 3849 4840 6136 6256 12790 13476 14267 14515 Size: 2120 991 1296 120 6534 686 791 248 Cuts at: 14515 1729 Size: 4049 Fragments arranged by size: 6534 4049 2120 1296 991 791 686 248 120 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 16835 SgrAI Cr'CCGG_yG Cuts at: 11605 12907 13165 11605 Size: 1302 258 15275 Fragments arranged by size: 15275 1302 258 SmaI CCC'GGG Cuts at: 457 1948 6478 6571 8893 9568 13681 13857 457 Size: 1491 4530 93 2322 675 4113 176 3435 Fragments arranged by size: 4530 4113 3435 2322 1491 675 176 93 SmlI C'TyrA_G Cuts at: 1252 1564 3451 3523 3562 3742 3793 4066 5095 Size: 312 1887 72 39 180 51 273 1029 Cuts at: 5095 5731 5755 6916 7123 7162 7342 7600 10180 Size: 636 24 1161 207 39 180 258 2580 Cuts at: 10180 11167 11218 11317 13513 13990 14905 15397 15659 Size: 987 51 99 2196 477 915 492 262 Cuts at: 15659 1252 Size: 2428 Fragments arranged by size: 2580 2428 2196 1887 1161 1029 987 915 636 492 477 312 273 262 258 207 180 180 99 72 51 51 39 39 24 SpeI A'CTAG_T Cuts at: 67 67 Size: 16835 SphI G_CATG'C Cuts at: 4380 13888 14388 4380 Size: 9508 500 6827 Fragments arranged by size: 9508 6827 500 SrfI GCCC'GGGC Cuts at: 6571 6571 Size: 16835 StuI AGG'CCT Cuts at: 853 1868 2537 2992 3181 3577 5809 9727 12658 Size: 1015 669 455 189 396 2232 3918 2931 Cuts at: 12658 13135 13252 853 Size: 477 117 4436 Fragments arranged by size: 4436 3918 2931 2232 1015 669 477 455 396 189 117 StyI C'CwwG_G Cuts at: 204 588 668 1181 1205 2012 2552 3572 3842 Size: 384 80 513 24 807 540 1020 270 Cuts at: 3842 4097 4493 5462 6236 7106 7763 8738 8855 Size: 255 396 969 774 870 657 975 117 Cuts at: 8855 9709 9722 10283 10715 11030 11867 12017 12300 Size: 854 13 561 432 315 837 150 283 Cuts at: 12300 12449 13601 13969 14323 204 Size: 149 1152 368 354 2716 Fragments arranged by size: 2716 1152 1020 975 969 870 854 837 807 774 657 561 540 513 432 396 384 368 354 315 283 270 255 150 149 117 80 24 13 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1770 2597 3787 6796 7474 9907 12445 14418 14551 Size: 827 1190 3009 678 2433 2538 1973 133 Cuts at: 14551 15010 15219 1770 Size: 459 209 3386 Fragments arranged by size: 3386 3009 2538 2433 1973 1190 827 678 459 209 133 TatI w'GTAC_w Cuts at: 1649 2516 4010 4184 9968 10859 16350 1649 Size: 867 1494 174 5784 891 5491 2134 Fragments arranged by size: 5784 5491 2134 1494 891 867 174 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1279 1311 2062 2094 7478 7510 10918 10950 11193 Size: 32 751 32 5384 32 3408 32 243 Cuts at: 11193 11225 12703 12735 16352 16384 1279 Size: 32 1478 32 3617 32 1730 Fragments arranged by size: 5384 3617 3408 1730 1478 751 243 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 13192 14632 13192 Size: 1440 15395 XbaI T'CTAG_A Cuts at: 13866 13866 Size: 16835 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1392 5550 7548 10137 10377 11874 12984 14330 1392 Size: 4158 1998 2589 240 1497 1110 1346 3897 Fragments arranged by size: 4158 3897 2589 1998 1497 1346 1110 240 XhoI C'TCGA_G Cuts at: 3562 3793 11167 11218 3562 Size: 231 7374 51 9179 Fragments arranged by size: 9179 7374 231 51 XmaI C'CCGG_G Cuts at: 455 1946 6476 6569 8891 9566 13679 13855 455 Size: 1491 4530 93 2322 675 4113 176 3435 Fragments arranged by size: 4530 4113 3435 2322 1491 675 176 93 XmnI GAAnn'nnTTC Cuts at: 328 13252 328 Size: 12924 3911 ZraI GAC'GTC Cuts at: 8898 12578 8898 Size: 3680 13155 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI SrfI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AflII AscI AvrII BaeI BfrBI BmtI BstZ17I FspAI HpaI MfeI NdeI NheI NruI NsiI PacI PsiI SnaBI SspI SwaI