Restriction map for
pF1KE2502
(Circular) (Six-base) MAPSORT of: pF1KE2502.seq Check: 9561 from: 1 to: 5285
pF1KE2502 5285 bp
With 182 enzymes: SgfI *
February 16, 2012 13:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 637 1357 637
Size: 720 4565
AccI GT'mk_AC
Cuts at: 737 947 2323 737
Size: 210 1376 3699
Fragments arranged by size:
3699 1376 210
Acc65I G'GTAC_C
Cuts at: 2301 2301
Size: 5285
AclI AA'CG_TT
Cuts at: 5125 5125
Size: 5285
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 577 589 787 1195 1387 3110 3542 4289 4557
Size: 12 198 408 192 1723 432 747 268
Cuts at: 4557 577
Size: 1305
Fragments arranged by size:
1723 1305 747 432 408 268 198 192
12
AflIII A'CryG_T
Cuts at: 2637 3741 4718 2637
Size: 1104 977 3204
Fragments arranged by size:
3204 1104 977
AgeI A'CCGG_T
Cuts at: 3673 3673
Size: 5285
AleI CACnn'nnGTG
Cuts at: 1751 2041 1751
Size: 290 4995
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1621 1915 1621
Size: 294 4991
AlwNI CAG_nnn'CTG
Cuts at: 1425 2590 4157 1425
Size: 1165 1567 2553
Fragments arranged by size:
2553 1567 1165
ApaI G_GGCC'C
Cuts at: 1146 1146
Size: 5285
ApaLI G'TGCA_C
Cuts at: 1950 4055 1950
Size: 2105 3180
ApoI r'AATT_y
Cuts at: 859 2289 4638 4894 859
Size: 1430 2349 256 1250
Fragments arranged by size:
2349 1430 1250 256
AseI AT'TA_AT
Cuts at: 20 1813 20
Size: 1793 3492
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5285
AvaI C'yCGr_G
Cuts at: 289 359 1235 2161 2305 289
Size: 70 876 926 144 3269
Fragments arranged by size:
3269 926 876 144 70
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2229 2262 2229
Size: 33 5252
BamHI G'GATC_C
Cuts at: 2099 2310 2099
Size: 211 5074
BanI G'GyrC_C
Cuts at: 958 1230 1605 1799 2301 2715 2963 2998 958
Size: 272 375 194 502 414 248 35 3245
Fragments arranged by size:
3245 502 414 375 272 248 194 35
BanII G_rGCy'C
Cuts at: 296 1146 1214 2299 3329 296
Size: 850 68 1085 1030 2252
Fragments arranged by size:
2252 1085 1030 850 68
BbeI G_GCGC'C
Cuts at: 1803 2719 2967 1803
Size: 916 248 4121
Fragments arranged by size:
4121 916 248
BbvCI CC'TCA_GC
Cuts at: 1090 1090
Size: 5285
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5251
BciVI GTATCCnnnnn_n'
Cuts at: 124 3183 3944 5285 124
Size: 3059 761 1341 124
Fragments arranged by size:
3059 1341 761 124
BclI T'GATC_A
Cuts at: 2805 2805
Size: 5285
BglI GCCn_nnn'nGGC
Cuts at: 1670 1670
Size: 5285
BglII A'GATC_T
Cuts at: 770 1109 1658 2800 4837 770
Size: 339 549 1142 2037 1218
Fragments arranged by size:
2037 1218 1142 549 339
BlpI GC'TnA_GC
Cuts at: 2397 2397
Size: 5285
Bme1580I G_kGCm'C
Cuts at: 637 1146 1235 1610 1844 1853 1954 2910 3003
Size: 509 89 375 234 9 101 956 93
Cuts at: 3003 4059 637
Size: 1056 1863
Fragments arranged by size:
1863 1056 956 509 375 234 101 93
89 9
BmrI ACTGGGnnnn_n'
Cuts at: 318 974 1435 1805 2497 2578 2913 5057 318
Size: 656 461 370 692 81 335 2144 546
Fragments arranged by size:
2144 692 656 546 461 370 335 81
BmtI G_CTAG'C
Cuts at: 1408 1408
Size: 5285
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3652 3652
Size: 5285
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 143 320 143
Size: 177 5108
Bpu10I CC'TnA_GC
Cuts at: 1090 1593 1090
Size: 503 4782
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2461 3340 3832 4130 2461
Size: 879 492 298 3616
Fragments arranged by size:
3616 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 169 1507 1534 4989 169
Size: 1338 27 3455 465
Fragments arranged by size:
3455 1338 465 27
BsaAI yAC'GTr
Cuts at: 1007 2638 1007
Size: 1631 3654
BsaBI GATnn'nnATC
Cuts at: 6 2799 4836 6
Size: 2793 2037 455
Fragments arranged by size:
2793 2037 455
BsaHI Gr'CG_yC
Cuts at: 902 1169 1772 1800 2716 2964 3666 5162 902
Size: 267 603 28 916 248 702 1496 1025
Fragments arranged by size:
1496 1025 916 702 603 267 248 28
BsaWI w'CCGG_w
Cuts at: 2102 2474 2995 3673 3947 4094 4505 2102
Size: 372 521 678 274 147 411 2882
Fragments arranged by size:
2882 678 521 411 372 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 334 364 334
Size: 30 5255
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 355 812 1873 355
Size: 457 1061 3767
Fragments arranged by size:
3767 1061 457
BseYI C'CCAG_C
Cuts at: 154 394 870 917 2710 3468 4045 4541 154
Size: 240 476 47 1793 758 577 496 898
Fragments arranged by size:
1793 898 758 577 496 476 240 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1729 1729
Size: 5285
BsiEI CG_ry'CG
Cuts at: 104 1048 2827 2873 4081 104
Size: 944 1779 46 1208 1308
Fragments arranged by size:
1779 1308 1208 944 46
BsiHKAI G_wGCw'C
Cuts at: 296 1954 2299 3077 3267 4059 296
Size: 1658 345 778 190 792 1522
Fragments arranged by size:
1658 1522 792 778 345 190
Bsp1286I G_dGCh'C
Cuts at: 296 637 1146 1214 1235 1610 1844 1853 1954
Size: 341 509 68 21 375 234 9 101
Cuts at: 1954 2299 2910 3003 3077 3267 3329 4059 296
Size: 345 611 93 74 190 62 730 1522
Fragments arranged by size:
1522 730 611 509 375 345 341 234
190 101 93 74 68 62 21 9
BspEI T'CCGG_A
Cuts at: 2102 4505 2102
Size: 2403 2882
BspHI T'CATG_A
Cuts at: 4461 4461
Size: 5285
BspMI ACCTGCnnnn'nnnn_
Cuts at: 533 637 1091 1357 2335 2549 2851 3232 533
Size: 104 454 266 978 214 302 381 2586
Fragments arranged by size:
2586 978 454 381 302 266 214 104
BsrBI CCG'CTC
Cuts at: 50 1462 3577 3631 5115 5280 50
Size: 1412 2115 54 1484 165 55
Fragments arranged by size:
2115 1484 1412 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3197 3197
Size: 5285
BsrFI r'CCGG_y
Cuts at: 191 1033 1047 1580 3283 3464 3673 191
Size: 842 14 533 1703 181 209 1803
Fragments arranged by size:
1803 1703 842 533 209 181 14
BsrGI T'GTAC_A
Cuts at: 845 1586 845
Size: 741 4544
BssSI C'ACGA_G
Cuts at: 3556 3914 3556
Size: 358 4927
BstAPI GCAn_nnn'nTGC
Cuts at: 2662 2662
Size: 5285
BstBI TT'CG_AA
Cuts at: 3646 3646
Size: 5285
BstEII G'GTnAC_C
Cuts at: 1326 1326
Size: 5285
BstXI CCAn_nnnn'nTGG
Cuts at: 162 1686 162
Size: 1524 3761
BstYI r'GATC_y
Cuts at: 1 761 770 1109 1658 2099 2310 2777 2800
Size: 760 9 339 549 441 211 467 23
Cuts at: 2800 3135 3381 4393 4479 4491 4837 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 760 549 467 449 441 346 339
335 246 211 86 23 12 9
Bsu36I CC'TnA_GG
Cuts at: 887 1545 887
Size: 658 4627
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 450 687 3235 3416 3579 450
Size: 237 2548 181 163 2156
Fragments arranged by size:
2548 2156 237 181 163
BtsI GCAGTG_nn'
Cuts at: 635 2528 635
Size: 1893 3392
DraI TTT'AAA
Cuts at: 2284 2284
Size: 5285
DraIII CAC_nnn'GTG
Cuts at: 585 585
Size: 5285
DrdI GACnn_nn'nnGTC
Cuts at: 2991 3849 2991
Size: 858 4427
EaeI y'GGCC_r
Cuts at: 587 836 1045 1761 2870 3044 3435 3462 4807
Size: 249 209 716 1109 174 391 27 1345
Cuts at: 4807 587
Size: 1065
Fragments arranged by size:
1345 1109 1065 716 391 249 209 174
27
EagI C'GGCC_G
Cuts at: 1045 2870 1045
Size: 1825 3460
EarI CTCTTCn'nnn_
Cuts at: 2087 3308 3518 2087
Size: 1221 210 3854
Fragments arranged by size:
3854 1221 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3493 3803 3949 5095 3493
Size: 310 146 1146 3683
Fragments arranged by size:
3683 1146 310 146
EcoICRI GAG'CTC
Cuts at: 294 2297 294
Size: 2003 3282
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 143 320 577 589 787 1195 1387 3110 3542
Size: 177 257 12 198 408 192 1723 432
Cuts at: 3542 4289 4557 143
Size: 747 268 871
Fragments arranged by size:
1723 871 747 432 408 268 257 198
192 177 12
EcoNI CCTnn'n_nnAGG
Cuts at: 571 571
Size: 5285
EcoO109I rG'GnC_Cy
Cuts at: 150 441 459 1142 1549 2197 2424 150
Size: 291 18 683 407 648 227 3011
Fragments arranged by size:
3011 683 648 407 291 227 18
EcoRI G'AATT_C
Cuts at: 859 2289 859
Size: 1430 3855
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3304 3304
Size: 5285
FspI TGC'GCA
Cuts at: 385 3066 385
Size: 2681 2604
HaeII r_GCGC'y
Cuts at: 349 486 1803 2719 2967 3989 4968 349
Size: 137 1317 916 248 1022 979 666
Fragments arranged by size:
1317 1022 979 916 666 248 137
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 975 1007 1201 1233 1971 2003 3127 3157 3159
Size: 32 194 32 738 32 1124 30 2
Cuts at: 3159 3189 975
Size: 30 3071
Fragments arranged by size:
3071 1124 738 194 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 738 2112 2324 738
Size: 1374 212 3699
Fragments arranged by size:
3699 1374 212
KasI G'GCGC_C
Cuts at: 1799 2715 2963 1799
Size: 916 248 4121
Fragments arranged by size:
4121 916 248
KpnI G_GTAC'C
Cuts at: 2305 2305
Size: 5285
MluI A'CGCG_T
Cuts at: 4718 4718
Size: 5285
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3956 4140 3956
Size: 184 5101
MscI TGG'CCA
Cuts at: 589 838 3046 589
Size: 249 2208 2828
Fragments arranged by size:
2828 2208 249
MslI CAynn'nnrTG
Cuts at: 205 1228 1324 1684 1751 2041 3274 3401 205
Size: 1023 96 360 67 290 1233 127 2089
Fragments arranged by size:
2089 1233 1023 360 290 127 96 67
MspA1I CmG'CkG
Cuts at: 398 753 1825 2396 2710 3070 4083 4328 398
Size: 355 1072 571 314 360 1013 245 1355
Fragments arranged by size:
1355 1072 1013 571 360 355 314 245
NaeI GCC'GGC
Cuts at: 193 1049 3466 193
Size: 856 2417 2012
Fragments arranged by size:
2417 2012 856
NarI GG'CG_CC
Cuts at: 1800 2716 2964 1800
Size: 916 248 4121
Fragments arranged by size:
4121 916 248
NgoMIV G'CCGG_C
Cuts at: 191 1047 3464 191
Size: 856 2417 2012
Fragments arranged by size:
2417 2012 856
NheI G'CTAG_C
Cuts at: 1404 1404
Size: 5285
NspI r_CATG'y
Cuts at: 162 2338 2838 3745 162
Size: 2176 500 907 1702
Fragments arranged by size:
2176 1702 907 500
PciI A'CATG_T
Cuts at: 3741 3741
Size: 5285
PflMI CCAn_nnn'nTGG
Cuts at: 585 1272 1425 2022 585
Size: 687 153 597 3848
Fragments arranged by size:
3848 687 597 153
PfoI T'CCnGG_A
Cuts at: 1265 1265
Size: 5285
PmeI GTTT'AAAC
Cuts at: 2284 2284
Size: 5285
PmlI CAC'GTG
Cuts at: 1007 1007
Size: 5285
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4424 4456 4424
Size: 32 5253
PpuMI rG'GwC_Cy
Cuts at: 150 459 2197 150
Size: 309 1738 3238
Fragments arranged by size:
3238 1738 309
PspOMI G'GGCC_C
Cuts at: 1142 1142
Size: 5285
PstI C_TGCA'G
Cuts at: 2332 3017 2332
Size: 685 4600
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5285
PvuII CAG'CTG
Cuts at: 398 753 1825 2710 3070 398
Size: 355 1072 885 360 2613
Fragments arranged by size:
2613 1072 885 360 355
RsrII CG'GwC_CG
Cuts at: 3480 3480
Size: 5285
SacI G_AGCT'C
Cuts at: 296 2299 296
Size: 2003 3282
SalI G'TCGA_C
Cuts at: 736 2322 736
Size: 1586 3699
SanDI GG'GwC_CC
Cuts at: 150 2197 150
Size: 2047 3238
SapI GCTCTTCn'nnn_
Cuts at: 3308 3518 3308
Size: 210 5075
SbfI CC_TGCA'GG
Cuts at: 2332 2332
Size: 5285
ScaI AGT'ACT
Cuts at: 4802 4802
Size: 5285
SexAI A'CCwGG_T
Cuts at: 1397 1397
Size: 5285
SfcI C'TryA_G
Cuts at: 33 2328 3013 4006 4197 33
Size: 2295 685 993 191 1121
Fragments arranged by size:
2295 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1801 2717 2965 1801
Size: 916 248 4121
Fragments arranged by size:
4121 916 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5285
SmaI CCC'GGG
Cuts at: 1237 2307 1237
Size: 1070 4215
SmlI C'TyrA_G
Cuts at: 289 2161 2440 3355 3847 4109 289
Size: 1872 279 915 492 262 1465
Fragments arranged by size:
1872 1465 915 492 279 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5285
SphI G_CATG'C
Cuts at: 162 2338 2838 162
Size: 2176 500 2609
Fragments arranged by size:
2609 2176 500
StuI AGG'CCT
Cuts at: 691 691
Size: 5285
StyI C'CwwG_G
Cuts at: 545 974 1331 1409 2419 2773 545
Size: 429 357 78 1010 354 3057
Fragments arranged by size:
3057 1010 429 357 354 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 232 931 2868 3001 3460 3669 232
Size: 699 1937 133 459 209 1848
Fragments arranged by size:
1937 1848 699 459 209 133
TatI w'GTAC_w
Cuts at: 698 845 1586 2237 4800 698
Size: 147 741 651 2563 1183
Fragments arranged by size:
2563 1183 741 651 147
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 334 366 1546 1578 4802 4834 334
Size: 32 1180 32 3224 32 785
Fragments arranged by size:
3224 1180 785 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3082 3082
Size: 5285
XbaI T'CTAG_A
Cuts at: 2316 2316
Size: 5285
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 729 1221 2780 729
Size: 492 1559 3234
Fragments arranged by size:
3234 1559 492
XhoI C'TCGA_G
Cuts at: 289 2161 289
Size: 1872 3413
XmaI C'CCGG_G
Cuts at: 1235 2305 1235
Size: 1070 4215
XmnI GAAnn'nnTTC
Cuts at: 1372 1372
Size: 5285
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NheI NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BbsI
BfrBI BmgBI BsiWI BsmI BsmBI BssHII BstZ17I BtgI
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NotI NruI NsiI PacI PasI PshAI
PsiI PspXI PsrI SacII SfiI SgrAI SnaBI SrfI
SspI SwaI ZraI