Restriction map for
pF1KE2504
(Circular) (Six-base) MAPSORT of: pF1KE2504.seq Check: 6196 from: 1 to: 6155
pF1KE2504 6155 bp
With 182 enzymes: SgfI *
February 16, 2012 13:36 ..
AatII G_ACGT'C
Cuts at: 1052 1493 1052
Size: 441 5714
AccI GT'mk_AC
Cuts at: 3008 3193 3008
Size: 185 5970
Acc65I G'GTAC_C
Cuts at: 260 578 3171 260
Size: 318 2593 3244
Fragments arranged by size:
3244 2593 318
AclI AA'CG_TT
Cuts at: 5995 5995
Size: 6155
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 642 712 910 1244 1498 1749 2170 2552 2774
Size: 70 198 334 254 251 421 382 222
Cuts at: 2774 2923 2966 3124 3980 4412 5159 5427 642
Size: 149 43 158 856 432 747 268 1370
Fragments arranged by size:
1370 856 747 432 421 382 334 268
254 251 222 198 158 149 70 43
AflIII A'CryG_T
Cuts at: 2774 2891 3507 4611 5588 2774
Size: 117 616 1104 977 3341
Fragments arranged by size:
3341 1104 977 616 117
AgeI A'CCGG_T
Cuts at: 4543 4543
Size: 6155
AlwNI CAG_nnn'CTG
Cuts at: 1573 1609 1830 1871 2295 3460 5027 1573
Size: 36 221 41 424 1165 1567 2701
Fragments arranged by size:
2701 1567 1165 424 221 41 36
ApaLI G'TGCA_C
Cuts at: 4925 4925
Size: 6155
ApoI r'AATT_y
Cuts at: 1120 1995 3124 3159 5508 5764 1120
Size: 875 1129 35 2349 256 1511
Fragments arranged by size:
2349 1511 1129 875 256 35
AscI GG'CGCG_CC
Cuts at: 159 159
Size: 6155
AseI AT'TA_AT
Cuts at: 20 20
Size: 6155
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6155
AvaI C'yCGr_G
Cuts at: 628 1002 1300 1351 3175 628
Size: 374 298 51 1824 3608
Fragments arranged by size:
3608 1824 374 298 51
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 244 277 244
Size: 33 6122
BamHI G'GATC_C
Cuts at: 1707 2469 2720 3180 1707
Size: 762 251 460 4682
Fragments arranged by size:
4682 762 460 251
BanI G'GyrC_C
Cuts at: 260 302 578 976 2086 3171 3585 3833 3868
Size: 42 276 398 1110 1085 414 248 35
Cuts at: 3868 260
Size: 2547
Fragments arranged by size:
2547 1110 1085 414 398 276 248 42
35
BanII G_rGCy'C
Cuts at: 3169 4199 3169
Size: 1030 5125
BbeI G_GCGC'C
Cuts at: 3589 3837 3589
Size: 248 5907
BbsI GAAGACnn'nnnn_
Cuts at: 110 373 436 1008 1755 2728 110
Size: 263 63 572 747 973 3537
Fragments arranged by size:
3537 973 747 572 263 63
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 619 653 954 988 619
Size: 34 301 34 5786
Fragments arranged by size:
5786 301 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2018 4053 4814 6155 2018
Size: 2035 761 1341 2018
Fragments arranged by size:
2035 2018 1341 761
BclI T'GATC_A
Cuts at: 3675 3675
Size: 6155
BglI GCCn_nnn'nGGC
Cuts at: 823 1201 1381 823
Size: 378 180 5597
Fragments arranged by size:
5597 378 180
BglII A'GATC_T
Cuts at: 3670 5707 3670
Size: 2037 4118
BlpI GC'TnA_GC
Cuts at: 2209 3267 2209
Size: 1058 5097
Bme1580I G_kGCm'C
Cuts at: 981 3780 3873 4929 981
Size: 2799 93 1056 2207
Fragments arranged by size:
2799 2207 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1052 3367 3448 3783 5927 1052
Size: 2315 81 335 2144 1280
Fragments arranged by size:
2315 2144 1280 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1017 4522 1017
Size: 3505 2650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 401 1468 1765 1852 2050 401
Size: 1067 297 87 198 4506
Fragments arranged by size:
4506 1067 297 198 87
Bpu10I CC'TnA_GC
Cuts at: 2440 3092 2440
Size: 652 5503
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 1126 3331 4210 4702 5000 634
Size: 492 2205 879 492 298 1789
Fragments arranged by size:
2205 1789 879 492 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2814 2840 5859 2814
Size: 26 3019 3110
Fragments arranged by size:
3110 3019 26
BsaAI yAC'GTr
Cuts at: 479 3508 479
Size: 3029 3126
BsaBI GATnn'nnATC
Cuts at: 6 214 3669 5706 6
Size: 208 3455 2037 455
Fragments arranged by size:
3455 2037 455 208
BsaHI Gr'CG_yC
Cuts at: 1049 1490 3586 3834 4536 6032 1049
Size: 441 2096 248 702 1496 1172
Fragments arranged by size:
2096 1496 1172 702 441 248
BsaWI w'CCGG_w
Cuts at: 206 1094 3344 3865 4543 4817 4964 5375 206
Size: 888 2250 521 678 274 147 411 986
Fragments arranged by size:
2250 986 888 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 253 283 1688 1718 2794 2824 253
Size: 30 1405 30 1076 30 3584
Fragments arranged by size:
3584 1405 1076 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 398 625 1242 1709 398
Size: 227 617 467 4844
Fragments arranged by size:
4844 617 467 227
BseYI C'CCAG_C
Cuts at: 615 2716 3580 4338 4915 5411 615
Size: 2101 864 758 577 496 1359
Fragments arranged by size:
2101 1359 864 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 1023 2401 244
Size: 779 1378 3998
Fragments arranged by size:
3998 1378 779
BsiEI CG_ry'CG
Cuts at: 104 3697 3743 4951 104
Size: 3593 46 1208 1308
Fragments arranged by size:
3593 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1946 3169 3947 4137 4929 1946
Size: 1223 778 190 792 3172
Fragments arranged by size:
3172 1223 792 778 190
BsmI GAATG_Cn'
Cuts at: 1003 2644 1003
Size: 1641 4514
BsmBI CGTCTCn'nnnn_
Cuts at: 1497 2843 1497
Size: 1346 4809
Bsp1286I G_dGCh'C
Cuts at: 981 1946 3169 3780 3873 3947 4137 4199 4929
Size: 965 1223 611 93 74 190 62 730
Cuts at: 4929 981
Size: 2207
Fragments arranged by size:
2207 1223 965 730 611 190 93 74
62
BspEI T'CCGG_A
Cuts at: 206 5375 206
Size: 5169 986
BspHI T'CATG_A
Cuts at: 1949 5331 1949
Size: 3382 2773
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2417 3205 3419 3721 4102 2417
Size: 788 214 302 381 4470
Fragments arranged by size:
4470 788 381 302 214
BsrBI CCG'CTC
Cuts at: 50 155 2358 4447 4501 5985 6150 50
Size: 105 2203 2089 54 1484 165 55
Fragments arranged by size:
2203 2089 1484 165 105 55 54
BsrDI GCAATG_nn'
Cuts at: 4067 4067
Size: 6155
BsrFI r'CCGG_y
Cuts at: 4153 4334 4543 4153
Size: 181 209 5765
Fragments arranged by size:
5765 209 181
BssHII G'CGCG_C
Cuts at: 159 159
Size: 6155
BssSI C'ACGA_G
Cuts at: 4426 4784 4426
Size: 358 5797
BstAPI GCAn_nnn'nTGC
Cuts at: 176 457 921 3532 176
Size: 281 464 2611 2799
Fragments arranged by size:
2799 2611 464 281
BstBI TT'CG_AA
Cuts at: 4516 4516
Size: 6155
BstEII G'GTnAC_C
Cuts at: 1107 1107
Size: 6155
BstXI CCAn_nnnn'nTGG
Cuts at: 1071 2338 1071
Size: 1267 4888
BstYI r'GATC_y
Cuts at: 1 1289 1707 2222 2291 2298 2469 2720 2789
Size: 1288 418 515 69 7 171 251 69
Cuts at: 2789 3180 3647 3670 4005 4251 5263 5349 5361
Size: 391 467 23 335 246 1012 86 12
Cuts at: 5361 5707 1
Size: 346 449
Fragments arranged by size:
1288 1012 515 467 449 418 391 346
335 251 246 171 86 69 69 23
12 7
BtgI C'CryG_G
Cuts at: 107 1199 2162 107
Size: 1092 963 4100
Fragments arranged by size:
4100 1092 963
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 752 1040 4105 4286 4449 752
Size: 288 3065 181 163 2458
Fragments arranged by size:
3065 2458 288 181 163
BtsI GCAGTG_nn'
Cuts at: 1052 2066 3398 1052
Size: 1014 1332 3809
Fragments arranged by size:
3809 1332 1014
ClaI AT'CG_AT
Cuts at: 2477 2609 2477
Size: 132 6023
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2694 2729 2694
Size: 35 6120
DraI TTT'AAA
Cuts at: 3154 3154
Size: 6155
DrdI GACnn_nn'nnGTC
Cuts at: 2602 3861 4719 2602
Size: 1259 858 4038
Fragments arranged by size:
4038 1259 858
EaeI y'GGCC_r
Cuts at: 212 1205 1373 2043 3740 3914 4305 4332 5677
Size: 993 168 670 1697 174 391 27 1345
Cuts at: 5677 212
Size: 690
Fragments arranged by size:
1697 1345 993 690 670 391 174 168
27
EagI C'GGCC_G
Cuts at: 3740 3740
Size: 6155
EarI CTCTTCn'nnn_
Cuts at: 2048 4178 4388 2048
Size: 2130 210 3815
Fragments arranged by size:
3815 2130 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 1205 1485 4363 4673 4819 5965 127
Size: 1078 280 2878 310 146 1146 317
Fragments arranged by size:
2878 1146 1078 317 310 280 146
EcoICRI GAG'CTC
Cuts at: 3167 3167
Size: 6155
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 401 642 712 910 1244 1468 1498 1749 1765
Size: 241 70 198 334 224 30 251 16
Cuts at: 1765 1852 2050 2170 2552 2774 2923 2966 3124
Size: 87 198 120 382 222 149 43 158
Cuts at: 3124 3980 4412 5159 5427 401
Size: 856 432 747 268 1129
Fragments arranged by size:
1129 856 747 432 382 334 268 251
241 224 222 198 198 158 149 120
87 70 43 30 16
EcoNI CCTnn'n_nnAGG
Cuts at: 2248 2728 2248
Size: 480 5675
EcoO109I rG'GnC_Cy
Cuts at: 1868 2118 3294 1868
Size: 250 1176 4729
Fragments arranged by size:
4729 1176 250
EcoRI G'AATT_C
Cuts at: 1120 3159 1120
Size: 2039 4116
EcoRV GAT'ATC
Cuts at: 2613 2613
Size: 6155
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2151 2214 2899 4174 2151
Size: 63 685 1275 4132
Fragments arranged by size:
4132 1275 685 63
FspI TGC'GCA
Cuts at: 1736 3936 1736
Size: 2200 3955
HaeII r_GCGC'y
Cuts at: 1196 2761 3589 3837 4859 5838 1196
Size: 1565 828 248 1022 979 1513
Fragments arranged by size:
1565 1513 1022 979 828 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 313 345 1281 1313 2064 2096 2283 2315 2794
Size: 32 936 32 751 32 187 32 479
Cuts at: 2794 2826 3997 4027 4029 4059 313
Size: 32 1171 30 2 30 2409
Fragments arranged by size:
2409 1171 936 751 479 187 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1364 2484 2520 3194 1364
Size: 1120 36 674 4325
Fragments arranged by size:
4325 1120 674 36
HindIII A'AGCT_T
Cuts at: 805 805
Size: 6155
KasI G'GCGC_C
Cuts at: 3585 3833 3585
Size: 248 5907
KpnI G_GTAC'C
Cuts at: 264 582 3175 264
Size: 318 2593 3244
Fragments arranged by size:
3244 2593 318
MluI A'CGCG_T
Cuts at: 5588 5588
Size: 6155
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 491 1304 4826 5010 491
Size: 813 3522 184 1636
Fragments arranged by size:
3522 1636 813 184
MscI TGG'CCA
Cuts at: 214 2045 3916 214
Size: 1831 1871 2453
Fragments arranged by size:
2453 1871 1831
MslI CAynn'nnrTG
Cuts at: 220 2128 2422 2512 4144 4271 220
Size: 1908 294 90 1632 127 2104
Fragments arranged by size:
2104 1908 1632 294 127 90
MspA1I CmG'CkG
Cuts at: 373 575 1011 1702 3266 3580 3940 4953 5198
Size: 202 436 691 1564 314 360 1013 245
Cuts at: 5198 373
Size: 1330
Fragments arranged by size:
1564 1330 1013 691 436 360 314 245
202
NaeI GCC'GGC
Cuts at: 4336 4336
Size: 6155
NarI GG'CG_CC
Cuts at: 3586 3834 3586
Size: 248 5907
NcoI C'CATG_G
Cuts at: 107 2162 107
Size: 2055 4100
NgoMIV G'CCGG_C
Cuts at: 4334 4334
Size: 6155
NspI r_CATG'y
Cuts at: 1741 2778 2895 3208 3708 4615 1741
Size: 1037 117 313 500 907 3281
Fragments arranged by size:
3281 1037 907 500 313 117
PasI CC'CwG_GG
Cuts at: 2730 2730
Size: 6155
PciI A'CATG_T
Cuts at: 2774 2891 4611 2774
Size: 117 1720 4318
Fragments arranged by size:
4318 1720 117
PfoI T'CCnGG_A
Cuts at: 1312 1484 1742 2294 2698 1312
Size: 172 258 552 404 4769
Fragments arranged by size:
4769 552 404 258 172
PmeI GTTT'AAAC
Cuts at: 3154 3154
Size: 6155
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5294 5326 5294
Size: 32 6123
PpuMI rG'GwC_Cy
Cuts at: 1868 2118 1868
Size: 250 5905
PstI C_TGCA'G
Cuts at: 1826 3202 3887 1826
Size: 1376 685 4094
Fragments arranged by size:
4094 1376 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6155
PvuII CAG'CTG
Cuts at: 1011 1702 3580 3940 1011
Size: 691 1878 360 3226
Fragments arranged by size:
3226 1878 691 360
RsrII CG'GwC_CG
Cuts at: 4350 4350
Size: 6155
SacI G_AGCT'C
Cuts at: 3169 3169
Size: 6155
SalI G'TCGA_C
Cuts at: 3192 3192
Size: 6155
SapI GCTCTTCn'nnn_
Cuts at: 4178 4388 4178
Size: 210 5945
SbfI CC_TGCA'GG
Cuts at: 3202 3202
Size: 6155
ScaI AGT'ACT
Cuts at: 5672 5672
Size: 6155
SfcI C'TryA_G
Cuts at: 33 671 1822 2430 3198 3883 4876 5067 33
Size: 638 1151 608 768 685 993 191 1121
Fragments arranged by size:
1151 1121 993 768 685 638 608 191
SfoI GGC'GCC
Cuts at: 3587 3835 3587
Size: 248 5907
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6155
SmaI CCC'GGG
Cuts at: 3177 3177
Size: 6155
SmlI C'TyrA_G
Cuts at: 649 1141 3310 4225 4717 4979 649
Size: 492 2169 915 492 262 1825
Fragments arranged by size:
2169 1825 915 492 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6155
SphI G_CATG'C
Cuts at: 3208 3708 3208
Size: 500 5655
StyI C'CwwG_G
Cuts at: 107 194 1546 1776 1925 2162 2198 2387 2883
Size: 87 1352 230 149 237 36 189 496
Cuts at: 2883 2933 3289 3643 107
Size: 50 356 354 2619
Fragments arranged by size:
2619 1352 496 356 354 237 230 189
149 87 50 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 427 965 1104 3738 3871 4330 4539 427
Size: 538 139 2634 133 459 209 2043
Fragments arranged by size:
2634 2043 538 459 209 139 133
TatI w'GTAC_w
Cuts at: 632 3071 5670 632
Size: 2439 2599 1117
Fragments arranged by size:
2599 2439 1117
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1525 1557 1688 1720 1992 2024 5672 5704 1525
Size: 32 131 32 272 32 3648 32 1976
Fragments arranged by size:
3648 1976 272 131 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2806 3952 2806
Size: 1146 5009
XbaI T'CTAG_A
Cuts at: 3186 3186
Size: 6155
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 409 1197 1410 1779 3650 409
Size: 788 213 369 1871 2914
Fragments arranged by size:
2914 1871 788 369 213
XmaI C'CCGG_G
Cuts at: 3175 3175
Size: 6155
XmnI GAAnn'nnTTC
Cuts at: 1762 1762
Size: 6155
ZraI GAC'GTC
Cuts at: 1050 1491 1050
Size: 441 5714
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AscI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AvrII BbvCI BfrBI BmgBI BmtI BsiWI BsrGI BstZ17I
Bsu36I DraIII FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI