Restriction map for
pF1KE2505
(Circular) (Six-base) MAPSORT of: pF1KE2505.seq Check: 2095 from: 1 to: 6233
pF1KE2505 6233 bp
With 182 enzymes: SgfI *
February 16, 2012 13:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 146 264 588 1576 2061 146
Size: 118 324 988 485 4318
Fragments arranged by size:
4318 988 485 324 118
AccI GT'mk_AC
Cuts at: 431 2423 3271 431
Size: 1992 848 3393
Fragments arranged by size:
3393 1992 848
Acc65I G'GTAC_C
Cuts at: 337 1312 3249 337
Size: 975 1937 3321
Fragments arranged by size:
3321 1937 975
AclI AA'CG_TT
Cuts at: 6073 6073
Size: 6233
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 753 821 1347 3050 4058 4490 5237 5505
Size: 595 68 526 1703 1008 432 747 268
Cuts at: 5505 158
Size: 886
Fragments arranged by size:
1703 1008 886 747 595 526 432 268
68
AflIII A'CryG_T
Cuts at: 1862 3585 4689 5666 1862
Size: 1723 1104 977 2429
Fragments arranged by size:
2429 1723 1104 977
AgeI A'CCGG_T
Cuts at: 4621 4621
Size: 6233
AhdI GACnn_n'nnGTC
Cuts at: 1893 1893
Size: 6233
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1090 1090
Size: 6233
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1234 1266 2137 2169 1234
Size: 32 871 32 5298
Fragments arranged by size:
5298 871 32 32
AlwNI CAG_nnn'CTG
Cuts at: 441 1120 2124 3028 3538 5105 441
Size: 679 1004 904 510 1567 1569
Fragments arranged by size:
1569 1567 1004 904 679 510
ApaI G_GGCC'C
Cuts at: 2204 2501 3074 2204
Size: 297 573 5363
Fragments arranged by size:
5363 573 297
ApaLI G'TGCA_C
Cuts at: 5003 5003
Size: 6233
ApoI r'AATT_y
Cuts at: 508 3056 3237 5586 5842 508
Size: 2548 181 2349 256 899
Fragments arranged by size:
2548 2349 899 256 181
AseI AT'TA_AT
Cuts at: 20 20
Size: 6233
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6233
AvaI C'yCGr_G
Cuts at: 296 1275 1433 2390 2500 2726 3253 296
Size: 979 158 957 110 226 527 3276
Fragments arranged by size:
3276 979 957 527 226 158 110
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2302 2335 2302
Size: 33 6200
BamHI G'GATC_C
Cuts at: 196 3086 3258 196
Size: 2890 172 3171
Fragments arranged by size:
3171 2890 172
BanI G'GyrC_C
Cuts at: 337 460 1012 1312 1519 2244 2394 2401 3172
Size: 123 552 300 207 725 150 7 771
Cuts at: 3172 3249 3663 3911 3946 337
Size: 77 414 248 35 2624
Fragments arranged by size:
2624 771 725 552 414 300 248 207
150 123 77 35 7
BanII G_rGCy'C
Cuts at: 974 2204 2277 2288 2501 2543 3074 3227 3247
Size: 1230 73 11 213 42 531 153 20
Cuts at: 3247 4277 974
Size: 1030 2930
Fragments arranged by size:
2930 1230 1030 531 213 153 73 42
20 11
BbeI G_GCGC'C
Cuts at: 2405 3667 3915 2405
Size: 1262 248 4723
Fragments arranged by size:
4723 1262 248
BbsI GAAGACnn'nnnn_
Cuts at: 233 759 1277 3038 233
Size: 526 518 1761 3428
Fragments arranged by size:
3428 1761 526 518
BbvCI CC'TCA_GC
Cuts at: 2531 2531
Size: 6233
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2086 2120 2219 2253 2086
Size: 34 99 34 6066
Fragments arranged by size:
6066 99 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2644 4131 4892 6233 2644
Size: 1487 761 1341 2644
Fragments arranged by size:
2644 1487 1341 761
BclI T'GATC_A
Cuts at: 1526 3753 1526
Size: 2227 4006
BglI GCCn_nnn'nGGC
Cuts at: 1092 1779 2199 1092
Size: 687 420 5126
Fragments arranged by size:
5126 687 420
BglII A'GATC_T
Cuts at: 3748 5785 3748
Size: 2037 4196
BlpI GC'TnA_GC
Cuts at: 782 968 1082 1261 1984 3345 782
Size: 186 114 179 723 1361 3670
Fragments arranged by size:
3670 1361 723 186 179 114
Bme1580I G_kGCm'C
Cuts at: 463 1015 1784 2185 2204 2501 2727 3074 3858
Size: 552 769 401 19 297 226 347 784
Cuts at: 3858 3951 5007 463
Size: 93 1056 1689
Fragments arranged by size:
1689 1056 784 769 552 401 347 297
226 93 19
BmrI ACTGGGnnnn_n'
Cuts at: 448 526 2195 2406 2804 3016 3445 3526 3861
Size: 78 1669 211 398 212 429 81 335
Cuts at: 3861 6005 448
Size: 2144 676
Fragments arranged by size:
2144 1669 676 429 398 335 212 211
81 78
BmtI G_CTAG'C
Cuts at: 1339 1339
Size: 6233
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4600 4600
Size: 6233
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 373 904 1482 1496 1796 2010 2190 3241
Size: 80 531 578 14 300 214 180 1051
Cuts at: 3241 293
Size: 3285
Fragments arranged by size:
3285 1051 578 531 300 214 180 80
14
Bpu10I CC'TnA_GC
Cuts at: 2531 2531
Size: 6233
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1638 2862 3409 4288 4780 5078 1638
Size: 1224 547 879 492 298 2793
Fragments arranged by size:
2793 1224 879 547 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 521 844 1801 2583 5937 521
Size: 323 957 782 3354 817
Fragments arranged by size:
3354 957 817 782 323
BsaAI yAC'GTr
Cuts at: 1865 3586 1865
Size: 1721 4512
BsaBI GATnn'nnATC
Cuts at: 6 1417 3016 3202 3747 5784 6
Size: 1411 1599 186 545 2037 455
Fragments arranged by size:
2037 1599 1411 545 455 186
BsaHI Gr'CG_yC
Cuts at: 651 1383 2302 2402 3664 3912 4614 6110 651
Size: 732 919 100 1262 248 702 1496 774
Fragments arranged by size:
1496 1262 919 774 732 702 248 100
BsaWI w'CCGG_w
Cuts at: 1787 2216 3422 3943 4621 4895 5042 5453 1787
Size: 429 1206 521 678 274 147 411 2567
Fragments arranged by size:
2567 1206 678 521 429 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 262 292 1114 1144 1494 1524 1934 1964 262
Size: 30 822 30 350 30 410 30 4531
Fragments arranged by size:
4531 822 410 350 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 159 271 283 535 604 2279 159
Size: 112 12 252 69 1675 4113
Fragments arranged by size:
4113 1675 252 112 69 12
BseYI C'CCAG_C
Cuts at: 551 823 843 988 1237 1838 2124 2668 3156
Size: 272 20 145 249 601 286 544 488
Cuts at: 3156 3658 4416 4993 5489 551
Size: 502 758 577 496 1295
Fragments arranged by size:
1295 758 601 577 544 502 496 488
286 272 249 145 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1078 1096 1172 2099 2498 2635 1078
Size: 18 76 927 399 137 4676
Fragments arranged by size:
4676 927 399 137 76 18
BsiEI CG_ry'CG
Cuts at: 104 3775 3821 5029 104
Size: 3671 46 1208 1308
Fragments arranged by size:
3671 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1460 2288 3227 3247 4025 4215 5007 1460
Size: 828 939 20 778 190 792 2686
Fragments arranged by size:
2686 939 828 792 778 190 20
BsmBI CGTCTCn'nnnn_
Cuts at: 2324 2324
Size: 6233
Bsp1286I G_dGCh'C
Cuts at: 463 974 1015 1460 1784 2185 2204 2277 2288
Size: 511 41 445 324 401 19 73 11
Cuts at: 2288 2501 2543 2727 3074 3227 3247 3858 3951
Size: 213 42 184 347 153 20 611 93
Cuts at: 3951 4025 4215 4277 5007 463
Size: 74 190 62 730 1689
Fragments arranged by size:
1689 730 611 511 445 401 347 324
213 190 184 153 93 74 73 62
42 41 20 19 11
BspEI T'CCGG_A
Cuts at: 2216 5453 2216
Size: 3237 2996
BspHI T'CATG_A
Cuts at: 398 543 5409 398
Size: 145 4866 1222
Fragments arranged by size:
4866 1222 145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 146 264 588 864 1145 1253 1576 2061 2354
Size: 118 324 276 281 108 323 485 293
Cuts at: 2354 3283 3497 3799 4180 146
Size: 929 214 302 381 2199
Fragments arranged by size:
2199 929 485 381 324 323 302 293
281 276 214 118 108
BsrBI CCG'CTC
Cuts at: 50 4525 4579 6063 6228 50
Size: 4475 54 1484 165 55
Fragments arranged by size:
4475 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 686 2109 4145 686
Size: 1423 2036 2774
Fragments arranged by size:
2774 2036 1423
BsrFI r'CCGG_y
Cuts at: 1159 1544 2941 2998 4231 4412 4621 1159
Size: 385 1397 57 1233 181 209 2771
Fragments arranged by size:
2771 1397 1233 385 209 181 57
BsrGI T'GTAC_A
Cuts at: 683 683
Size: 6233
BssSI C'ACGA_G
Cuts at: 2518 4504 4862 2518
Size: 1986 358 3889
Fragments arranged by size:
3889 1986 358
BstAPI GCAn_nnn'nTGC
Cuts at: 279 421 3610 279
Size: 142 3189 2902
Fragments arranged by size:
3189 2902 142
BstBI TT'CG_AA
Cuts at: 4594 4594
Size: 6233
BstEII G'GTnAC_C
Cuts at: 876 876
Size: 6233
BstXI CCAn_nnnn'nTGG
Cuts at: 456 744 1914 456
Size: 288 1170 4775
Fragments arranged by size:
4775 1170 288
BstYI r'GATC_y
Cuts at: 1 196 983 1412 1818 2468 2651 3086 3258
Size: 195 787 429 406 650 183 435 172
Cuts at: 3258 3725 3748 4083 4329 5341 5427 5439 5785
Size: 467 23 335 246 1012 86 12 346
Cuts at: 5785 1
Size: 449
Fragments arranged by size:
1012 787 650 467 449 435 429 406
346 335 246 195 183 172 86 23
12
Bsu36I CC'TnA_GG
Cuts at: 2250 2250
Size: 6233
BtgI C'CryG_G
Cuts at: 107 263 1394 1401 1451 1718 1848 1995 2487
Size: 156 1131 7 50 267 130 147 492
Cuts at: 2487 2842 3191 107
Size: 355 349 3149
Fragments arranged by size:
3149 1131 492 355 349 267 156 147
130 50 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2004 3211 4183 4364 4527 2004
Size: 1207 972 181 163 3710
Fragments arranged by size:
3710 1207 972 181 163
BtsI GCAGTG_nn'
Cuts at: 961 2510 3476 961
Size: 1549 966 3718
Fragments arranged by size:
3718 1549 966
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1433 1468 1433
Size: 35 6198
DraI TTT'AAA
Cuts at: 3232 3232
Size: 6233
DraIII CAC_nnn'GTG
Cuts at: 660 3108 3192 660
Size: 2448 84 3701
Fragments arranged by size:
3701 2448 84
DrdI GACnn_nn'nnGTC
Cuts at: 2307 2911 3939 4797 2307
Size: 604 1028 858 3743
Fragments arranged by size:
3743 1028 858 604
EaeI y'GGCC_r
Cuts at: 731 3039 3818 3992 4383 4410 5755 731
Size: 2308 779 174 391 27 1345 1209
Fragments arranged by size:
2308 1345 1209 779 391 174 27
EagI C'GGCC_G
Cuts at: 3818 3818
Size: 6233
EarI CTCTTCn'nnn_
Cuts at: 177 2531 4256 4466 177
Size: 2354 1725 210 1944
Fragments arranged by size:
2354 1944 1725 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1045 1916 3142 4441 4751 4897 6043 1045
Size: 871 1226 1299 310 146 1146 1235
Fragments arranged by size:
1299 1235 1226 1146 871 310 146
EcoICRI GAG'CTC
Cuts at: 2286 3225 3245 2286
Size: 939 20 5274
Fragments arranged by size:
5274 939 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 293 373 753 821 904 1347 1482 1496
Size: 135 80 380 68 83 443 135 14
Cuts at: 1496 1796 2010 2190 3050 3241 4058 4490 5237
Size: 300 214 180 860 191 817 432 747
Cuts at: 5237 5505 158
Size: 268 886
Fragments arranged by size:
886 860 817 747 443 432 380 300
268 214 191 180 135 135 83 80
68 14
EcoNI CCTnn'n_nnAGG
Cuts at: 2358 2358
Size: 6233
EcoO109I rG'GnC_Cy
Cuts at: 1242 1933 2201 2774 2904 3070 3372 1242
Size: 691 268 573 130 166 302 4103
Fragments arranged by size:
4103 691 573 302 268 166 130
EcoRI G'AATT_C
Cuts at: 508 3237 508
Size: 2729 3504
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 783 4252 783
Size: 3469 2764
FspI TGC'GCA
Cuts at: 4014 4014
Size: 6233
HaeII r_GCGC'y
Cuts at: 1331 2405 3667 3915 4937 5916 1331
Size: 1074 1262 248 1022 979 1648
Fragments arranged by size:
1648 1262 1074 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1114 1146 1494 1526 1879 1911 2273 2305 4075
Size: 32 348 32 353 32 362 32 1770
Cuts at: 4075 4105 4107 4137 1114
Size: 30 2 30 3210
Fragments arranged by size:
3210 1770 362 353 348 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1488 2295 3272 1488
Size: 807 977 4449
Fragments arranged by size:
4449 977 807
KasI G'GCGC_C
Cuts at: 2401 3663 3911 2401
Size: 1262 248 4723
Fragments arranged by size:
4723 1262 248
KpnI G_GTAC'C
Cuts at: 341 1316 3253 341
Size: 975 1937 3321
Fragments arranged by size:
3321 1937 975
MluI A'CGCG_T
Cuts at: 5666 5666
Size: 6233
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 473 1157 4904 5088 473
Size: 684 3747 184 1618
Fragments arranged by size:
3747 1618 684 184
MscI TGG'CCA
Cuts at: 733 3041 3994 733
Size: 2308 953 2972
Fragments arranged by size:
2972 2308 953
MslI CAynn'nnrTG
Cuts at: 1072 1867 2827 3127 3133 4222 4349 1072
Size: 795 960 300 6 1089 127 2956
Fragments arranged by size:
2956 1089 960 795 300 127 6
MspA1I CmG'CkG
Cuts at: 375 608 967 1396 1403 1842 1988 2261 2489
Size: 233 359 429 7 439 146 273 228
Cuts at: 2489 2530 2686 2735 2844 3344 3658 4018 5031
Size: 41 156 49 109 500 314 360 1013
Cuts at: 5031 5276 375
Size: 245 1332
Fragments arranged by size:
1332 1013 500 439 429 360 359 314
273 245 233 228 156 146 109 49
41 7
NaeI GCC'GGC
Cuts at: 1161 4414 1161
Size: 3253 2980
NarI GG'CG_CC
Cuts at: 2402 3664 3912 2402
Size: 1262 248 4723
Fragments arranged by size:
4723 1262 248
NcoI C'CATG_G
Cuts at: 107 1718 1848 107
Size: 1611 130 4492
Fragments arranged by size:
4492 1611 130
NgoMIV G'CCGG_C
Cuts at: 1159 4412 1159
Size: 3253 2980
NheI G'CTAG_C
Cuts at: 1335 1335
Size: 6233
NspI r_CATG'y
Cuts at: 290 1284 3286 3786 4693 290
Size: 994 2002 500 907 1830
Fragments arranged by size:
2002 1830 994 907 500
PasI CC'CwG_GG
Cuts at: 192 2543 192
Size: 2351 3882
PciI A'CATG_T
Cuts at: 4689 4689
Size: 6233
PflMI CCAn_nnn'nTGG
Cuts at: 378 441 1293 1881 2564 3048 3169 3192 378
Size: 63 852 588 683 484 121 23 3419
Fragments arranged by size:
3419 852 683 588 484 121 63 23
PfoI T'CCnGG_A
Cuts at: 2866 2866
Size: 6233
PmeI GTTT'AAAC
Cuts at: 3232 3232
Size: 6233
PmlI CAC'GTG
Cuts at: 1865 1865
Size: 6233
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5372 5404 5372
Size: 32 6201
PpuMI rG'GwC_Cy
Cuts at: 1242 1933 2904 1242
Size: 691 971 4571
Fragments arranged by size:
4571 971 691
PshAI GACnn'nnGTC
Cuts at: 650 650
Size: 6233
PspOMI G'GGCC_C
Cuts at: 2200 2497 3070 2200
Size: 297 573 5363
Fragments arranged by size:
5363 573 297
PstI C_TGCA'G
Cuts at: 506 983 2058 2937 3096 3280 3965 506
Size: 477 1075 879 159 184 685 2774
Fragments arranged by size:
2774 1075 879 685 477 184 159
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6233
PvuII CAG'CTG
Cuts at: 375 967 1842 1988 2530 2686 3658 4018 375
Size: 592 875 146 542 156 972 360 2590
Fragments arranged by size:
2590 972 875 592 542 360 156 146
RsrII CG'GwC_CG
Cuts at: 2691 4428 2691
Size: 1737 4496
SacI G_AGCT'C
Cuts at: 2288 3227 3247 2288
Size: 939 20 5274
Fragments arranged by size:
5274 939 20
SacII CC_GC'GG
Cuts at: 1397 1404 2490 2845 1397
Size: 7 1086 355 4785
Fragments arranged by size:
4785 1086 355 7
SalI G'TCGA_C
Cuts at: 3270 3270
Size: 6233
SanDI GG'GwC_CC
Cuts at: 2904 2904
Size: 6233
SapI GCTCTTCn'nnn_
Cuts at: 2531 4256 4466 2531
Size: 1725 210 4298
Fragments arranged by size:
4298 1725 210
SbfI CC_TGCA'GG
Cuts at: 2058 3280 2058
Size: 1222 5011
ScaI AGT'ACT
Cuts at: 2416 5750 2416
Size: 3334 2899
SexAI A'CCwGG_T
Cuts at: 389 389
Size: 6233
SfcI C'TryA_G
Cuts at: 33 502 979 1175 1690 2054 2424 2933 3092
Size: 469 477 196 515 364 370 509 159
Cuts at: 3092 3276 3961 4954 5145 33
Size: 184 685 993 191 1121
Fragments arranged by size:
1121 993 685 515 509 477 469 370
364 196 191 184 159
SfoI GGC'GCC
Cuts at: 2403 3665 3913 2403
Size: 1262 248 4723
Fragments arranged by size:
4723 1262 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6233
SmaI CCC'GGG
Cuts at: 298 1435 2392 3255 298
Size: 1137 957 863 3276
Fragments arranged by size:
3276 1137 957 863
SmlI C'TyrA_G
Cuts at: 1653 2877 3388 4303 4795 5057 1653
Size: 1224 511 915 492 262 2829
Fragments arranged by size:
2829 1224 915 511 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6233
SphI G_CATG'C
Cuts at: 290 3286 3786 290
Size: 2996 500 2737
Fragments arranged by size:
2996 2737 500
SrfI GCCC'GGGC
Cuts at: 298 298
Size: 6233
StyI C'CwwG_G
Cuts at: 107 410 515 1040 1718 1848 1898 2558 3367
Size: 303 105 525 678 130 50 660 809
Cuts at: 3367 3721 107
Size: 354 2619
Fragments arranged by size:
2619 809 678 660 525 354 303 130
105 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 837 1663 2708 3816 3949 4408 4617 837
Size: 826 1045 1108 133 459 209 2453
Fragments arranged by size:
2453 1108 1045 826 459 209 133
TatI w'GTAC_w
Cuts at: 683 1859 2027 2414 2522 5748 683
Size: 1176 168 387 108 3226 1168
Fragments arranged by size:
3226 1176 1168 387 168 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 646 678 1934 1966 5750 5782 646
Size: 32 1256 32 3784 32 1097
Fragments arranged by size:
3784 1256 1097 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4030 4030
Size: 6233
XbaI T'CTAG_A
Cuts at: 3264 3264
Size: 6233
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 741 1263 1846 2175 3728 741
Size: 522 583 329 1553 3246
Fragments arranged by size:
3246 1553 583 522 329
XmaI C'CCGG_G
Cuts at: 296 1433 2390 3253 296
Size: 1137 957 863 3276
Fragments arranged by size:
3276 1137 957 863
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AscI AvrII BfrBI BmgBI
BsiWI BsmI BssHII BstZ17I ClaI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NotI NruI NsiI PacI
PsiI PspXI PsrI SfiI SgrAI SnaBI SspI StuI
SwaI XhoI XmnI ZraI