Restriction map for
pF1KE2515
(Circular) (Six-base) MAPSORT of: pF1KE2515.seq Check: 7989 from: 1 to: 4226
pF1KE2515 4226 bp
With 182 enzymes: SgfI *
February 25, 2014 15:45 ..
AatII G_ACGT'C
Cuts at: 392 392
Size: 4226
AccI GT'mk_AC
Cuts at: 1264 1264
Size: 4226
Acc65I G'GTAC_C
Cuts at: 1242 1242
Size: 4226
AclI AA'CG_TT
Cuts at: 4066 4066
Size: 4226
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 2051 2483 3230 3498 328
Size: 1723 432 747 268 1056
Fragments arranged by size:
1723 1056 747 432 268
AflIII A'CryG_T
Cuts at: 761 776 1578 2682 3659 761
Size: 15 802 1104 977 1328
Fragments arranged by size:
1328 1104 977 802 15
AgeI A'CCGG_T
Cuts at: 2614 2614
Size: 4226
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 447 479 447
Size: 32 4194
AlwNI CAG_nnn'CTG
Cuts at: 1531 3098 1531
Size: 1567 2659
ApaI G_GGCC'C
Cuts at: 441 441
Size: 4226
ApaLI G'TGCA_C
Cuts at: 949 2996 949
Size: 2047 2179
ApoI r'AATT_y
Cuts at: 144 1230 3579 3835 144
Size: 1086 2349 256 535
Fragments arranged by size:
2349 1086 535 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4226
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4226
AvaI C'yCGr_G
Cuts at: 396 647 1021 1246 396
Size: 251 374 225 3376
Fragments arranged by size:
3376 374 251 225
BamHI G'GATC_C
Cuts at: 1251 1251
Size: 4226
BanI G'GyrC_C
Cuts at: 359 400 663 1105 1242 1656 1904 1939 359
Size: 41 263 442 137 414 248 35 2646
Fragments arranged by size:
2646 442 414 263 248 137 41 35
BanII G_rGCy'C
Cuts at: 441 560 812 1240 2270 441
Size: 119 252 428 1030 2397
Fragments arranged by size:
2397 1030 428 252 119
BbeI G_GCGC'C
Cuts at: 667 1660 1908 667
Size: 993 248 2985
Fragments arranged by size:
2985 993 248
BbsI GAAGACnn'nnnn_
Cuts at: 902 1090 902
Size: 188 4038
BbvCI CC'TCA_GC
Cuts at: 239 239
Size: 4226
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 432 466 432
Size: 34 4192
BciVI GTATCCnnnnn_n'
Cuts at: 2124 2885 4226 2124
Size: 761 1341 2124
Fragments arranged by size:
2124 1341 761
BclI T'GATC_A
Cuts at: 1746 1746
Size: 4226
BglI GCCn_nnn'nGGC
Cuts at: 112 160 961 112
Size: 48 801 3377
Fragments arranged by size:
3377 801 48
BglII A'GATC_T
Cuts at: 1097 1741 3778 1097
Size: 644 2037 1545
Fragments arranged by size:
2037 1545 644
BlpI GC'TnA_GC
Cuts at: 1338 1338
Size: 4226
Bme1580I G_kGCm'C
Cuts at: 403 441 953 1851 1944 3000 403
Size: 38 512 898 93 1056 1629
Fragments arranged by size:
1629 1056 898 512 93 38
BmrI ACTGGGnnnn_n'
Cuts at: 556 1438 1519 1854 3998 556
Size: 882 81 335 2144 784
Fragments arranged by size:
2144 882 784 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2593 2593
Size: 4226
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 163
Size: 4226
Bpu10I CC'TnA_GC
Cuts at: 239 239
Size: 4226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1402 2281 2773 3071 1402
Size: 879 492 298 2557
Fragments arranged by size:
2557 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3930 3930
Size: 4226
BsaAI yAC'GTr
Cuts at: 519 948 1579 519
Size: 429 631 3166
Fragments arranged by size:
3166 631 429
BsaBI GATnn'nnATC
Cuts at: 6 1740 3777 6
Size: 1734 2037 455
Fragments arranged by size:
2037 1734 455
BsaHI Gr'CG_yC
Cuts at: 389 664 1657 1905 2607 4103 389
Size: 275 993 248 702 1496 512
Fragments arranged by size:
1496 993 702 512 275 248
BsaWI w'CCGG_w
Cuts at: 1211 1415 1936 2614 2888 3035 3446 1211
Size: 204 521 678 274 147 411 1991
Fragments arranged by size:
1991 678 521 411 274 204 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 256 286 325 355 748 778 256
Size: 30 39 30 393 30 3704
Fragments arranged by size:
3704 393 39 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 313 562 1108 313
Size: 249 546 3431
Fragments arranged by size:
3431 546 249
BseYI C'CCAG_C
Cuts at: 1651 2409 2986 3482 1651
Size: 758 577 496 2395
Fragments arranged by size:
2395 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1768 1814 3022 104
Size: 1664 46 1208 1308
Fragments arranged by size:
1664 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 560 641 953 1240 2018 2208 3000 560
Size: 81 312 287 778 190 792 1786
Fragments arranged by size:
1786 792 778 312 287 190 81
Bsp1286I G_dGCh'C
Cuts at: 403 441 560 641 812 953 1240 1851 1944
Size: 38 119 81 171 141 287 611 93
Cuts at: 1944 2018 2208 2270 3000 403
Size: 74 190 62 730 1629
Fragments arranged by size:
1629 730 611 287 190 171 141 119
93 81 74 62 38
BspEI T'CCGG_A
Cuts at: 3446 3446
Size: 4226
BspHI T'CATG_A
Cuts at: 3402 3402
Size: 4226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1276 1490 1792 2173 1276
Size: 214 302 381 3329
Fragments arranged by size:
3329 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2518 2572 4056 4221 50
Size: 2468 54 1484 165 55
Fragments arranged by size:
2468 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2138 2138
Size: 4226
BsrFI r'CCGG_y
Cuts at: 356 403 722 1003 2224 2405 2614 356
Size: 47 319 281 1221 181 209 1968
Fragments arranged by size:
1968 1221 319 281 209 181 47
BssSI C'ACGA_G
Cuts at: 295 2497 2855 295
Size: 2202 358 1666
Fragments arranged by size:
2202 1666 358
BstAPI GCAn_nnn'nTGC
Cuts at: 413 1603 413
Size: 1190 3036
BstBI TT'CG_AA
Cuts at: 2587 2587
Size: 4226
BstEII G'GTnAC_C
Cuts at: 522 843 522
Size: 321 3905
BstXI CCAn_nnnn'nTGG
Cuts at: 840 1164 840
Size: 324 3902
BstYI r'GATC_y
Cuts at: 1 743 1097 1251 1718 1741 2076 2322 3334
Size: 742 354 154 467 23 335 246 1012
Cuts at: 3334 3420 3432 3778 1
Size: 86 12 346 449
Fragments arranged by size:
1012 742 467 449 354 346 335 246
154 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4226
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2176 2357 2520 2176
Size: 181 163 3882
Fragments arranged by size:
3882 181 163
BtsI GCAGTG_nn'
Cuts at: 228 1469 228
Size: 1241 2985
DraI TTT'AAA
Cuts at: 1225 1225
Size: 4226
DraIII CAC_nnn'GTG
Cuts at: 522 522
Size: 4226
DrdI GACnn_nn'nnGTC
Cuts at: 1932 2790 1932
Size: 858 3368
EaeI y'GGCC_r
Cuts at: 207 710 797 1811 1985 2376 2403 3748 207
Size: 503 87 1014 174 391 27 1345 685
Fragments arranged by size:
1345 1014 685 503 391 174 87 27
EagI C'GGCC_G
Cuts at: 1811 1811
Size: 4226
EarI CTCTTCn'nnn_
Cuts at: 370 616 2249 2459 370
Size: 246 1633 210 2137
Fragments arranged by size:
2137 1633 246 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 229 2434 2744 2890 4036 229
Size: 2205 310 146 1146 419
Fragments arranged by size:
2205 1146 419 310 146
EcoICRI GAG'CTC
Cuts at: 558 1238 558
Size: 680 3546
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 328 2051 2483 3230 3498 163
Size: 165 1723 432 747 268 891
Fragments arranged by size:
1723 891 747 432 268 165
EcoNI CCTnn'n_nnAGG
Cuts at: 367 862 367
Size: 495 3731
EcoO109I rG'GnC_Cy
Cuts at: 437 1143 1365 437
Size: 706 222 3298
Fragments arranged by size:
3298 706 222
EcoRI G'AATT_C
Cuts at: 1230 1230
Size: 4226
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2245 2245
Size: 4226
FspI TGC'GCA
Cuts at: 322 2007 322
Size: 1685 2541
FspAI rTGC'GCAy
Cuts at: 322 322
Size: 4226
HaeII r_GCGC'y
Cuts at: 667 1660 1908 2930 3909 667
Size: 993 248 1022 979 984
Fragments arranged by size:
1022 993 984 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 235 267 379 411 2068 2098 2100 2130 235
Size: 32 112 32 1657 30 2 30 2331
Fragments arranged by size:
2331 1657 112 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1265 1265
Size: 4226
KasI G'GCGC_C
Cuts at: 663 1656 1904 663
Size: 993 248 2985
Fragments arranged by size:
2985 993 248
KpnI G_GTAC'C
Cuts at: 1246 1246
Size: 4226
MluI A'CGCG_T
Cuts at: 3659 3659
Size: 4226
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2897 3081 2897
Size: 184 4042
MscI TGG'CCA
Cuts at: 209 799 1987 209
Size: 590 1188 2448
Fragments arranged by size:
2448 1188 590
MslI CAynn'nnrTG
Cuts at: 1114 2215 2342 1114
Size: 1101 127 2998
Fragments arranged by size:
2998 1101 127
MspA1I CmG'CkG
Cuts at: 840 1182 1337 1651 2011 3024 3269 840
Size: 342 155 314 360 1013 245 1797
Fragments arranged by size:
1797 1013 360 342 314 245 155
NaeI GCC'GGC
Cuts at: 2407 2407
Size: 4226
NarI GG'CG_CC
Cuts at: 664 1657 1905 664
Size: 993 248 2985
Fragments arranged by size:
2985 993 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4226
NgoMIV G'CCGG_C
Cuts at: 2405 2405
Size: 4226
NspI r_CATG'y
Cuts at: 420 780 1119 1279 1779 2686 420
Size: 360 339 160 500 907 1960
Fragments arranged by size:
1960 907 500 360 339 160
PciI A'CATG_T
Cuts at: 776 2682 776
Size: 1906 2320
PmeI GTTT'AAAC
Cuts at: 1225 1225
Size: 4226
PmlI CAC'GTG
Cuts at: 519 519
Size: 4226
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3365 3397 3365
Size: 32 4194
PpuMI rG'GwC_Cy
Cuts at: 1143 1143
Size: 4226
PspOMI G'GGCC_C
Cuts at: 437 437
Size: 4226
PstI C_TGCA'G
Cuts at: 1273 1958 1273
Size: 685 3541
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4226
PvuII CAG'CTG
Cuts at: 840 1182 1651 2011 840
Size: 342 469 360 3055
Fragments arranged by size:
3055 469 360 342
RsrII CG'GwC_CG
Cuts at: 2421 2421
Size: 4226
SacI G_AGCT'C
Cuts at: 560 1240 560
Size: 680 3546
SalI G'TCGA_C
Cuts at: 1263 1263
Size: 4226
SapI GCTCTTCn'nnn_
Cuts at: 2249 2459 2249
Size: 210 4016
SbfI CC_TGCA'GG
Cuts at: 1273 1273
Size: 4226
ScaI AGT'ACT
Cuts at: 3743 3743
Size: 4226
SfcI C'TryA_G
Cuts at: 33 1269 1954 2947 3138 33
Size: 1236 685 993 191 1121
Fragments arranged by size:
1236 1121 993 685 191
SfoI GGC'GCC
Cuts at: 665 1658 1906 665
Size: 993 248 2985
Fragments arranged by size:
2985 993 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4226
SmaI CCC'GGG
Cuts at: 649 1248 649
Size: 599 3627
SmlI C'TyrA_G
Cuts at: 1381 2296 2788 3050 1381
Size: 915 492 262 2557
Fragments arranged by size:
2557 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4226
SphI G_CATG'C
Cuts at: 1119 1279 1779 1119
Size: 160 500 3566
Fragments arranged by size:
3566 500 160
StyI C'CwwG_G
Cuts at: 107 158 428 1360 1714 107
Size: 51 270 932 354 2619
Fragments arranged by size:
2619 932 354 270 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1809 1942 2401 2610 1809
Size: 133 459 209 3425
Fragments arranged by size:
3425 459 209 133
TatI w'GTAC_w
Cuts at: 728 3741 728
Size: 3013 1213
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3743 3775 3743
Size: 32 4194
Tth111I GACn'n_nGTC
Cuts at: 2023 2023
Size: 4226
XbaI T'CTAG_A
Cuts at: 1257 1257
Size: 4226
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1721 1721
Size: 4226
XmaI C'CCGG_G
Cuts at: 647 1246 647
Size: 599 3627
ZraI GAC'GTC
Cuts at: 390 390
Size: 4226
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AscI AvrII
BaeI BfrBI BmgBI BmtI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoRV FseI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI