Restriction map for
pF1KE2530
(Circular) (Six-base) MAPSORT of: pF1KE2530.seq Check: 1423 from: 1 to: 12479
pF1KE2530 12479 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 492 492
Size: 12479
AatII G_ACGT'C
Cuts at: 2414 6898 2414
Size: 4484 7995
AccI GT'mk_AC
Cuts at: 2296 9517 2296
Size: 7221 5258
Acc65I G'GTAC_C
Cuts at: 6794 7178 9495 6794
Size: 384 2317 9778
Fragments arranged by size:
9778 2317 384
AclI AA'CG_TT
Cuts at: 2933 9473 12319 2933
Size: 6540 2846 3093
Fragments arranged by size:
6540 3093 2846
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1178 1284 2066 2184 2531 3656 3691 5242 5348
Size: 106 782 118 347 1125 35 1551 106
Cuts at: 5348 6004 6015 6322 6539 7594 8565 9400 10304
Size: 656 11 307 217 1055 971 835 904
Cuts at: 10304 10736 11483 11751 1178
Size: 432 747 268 1906
Fragments arranged by size:
1906 1551 1125 1055 971 904 835 782
747 656 432 347 307 268 217 118
106 106 35 11
AflIII A'CryG_T
Cuts at: 277 321 1045 1282 1962 6753 6990 8905 9831
Size: 44 724 237 680 4791 237 1915 926
Cuts at: 9831 10935 11912 277
Size: 1104 977 844
Fragments arranged by size:
4791 1915 1104 977 926 844 724 680
237 237 44
AgeI A'CCGG_T
Cuts at: 8947 10867 8947
Size: 1920 10559
AhdI GACnn_n'nnGTC
Cuts at: 2751 8351 2751
Size: 5600 6879
AleI CACnn'nnGTG
Cuts at: 2447 2447
Size: 12479
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 629 3998 4814 629
Size: 3369 816 8294
Fragments arranged by size:
8294 3369 816
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1952 1984 8706 8738 1952
Size: 32 6722 32 5693
Fragments arranged by size:
6722 5693 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2266 3602 5179 9784 11351 2266
Size: 1336 1577 4605 1567 3394
Fragments arranged by size:
4605 3394 1577 1567 1336
ApaI G_GGCC'C
Cuts at: 4992 4992
Size: 12479
ApaLI G'TGCA_C
Cuts at: 3239 3507 4839 11249 3239
Size: 268 1332 6410 4469
Fragments arranged by size:
6410 4469 1332 268
ApoI r'AATT_y
Cuts at: 808 2125 2145 3358 3562 4146 5146 5244 6408
Size: 1317 20 1213 204 584 1000 98 1164
Cuts at: 6408 6676 7056 7394 7607 8110 8684 9001 9007
Size: 268 380 338 213 503 574 317 6
Cuts at: 9007 9483 11832 12088 808
Size: 476 2349 256 1199
Fragments arranged by size:
2349 1317 1213 1199 1164 1000 584 574
503 476 380 338 317 268 256 213
204 98 20 6
AseI AT'TA_AT
Cuts at: 20 1244 20
Size: 1224 11255
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 12479
AvaI C'yCGr_G
Cuts at: 157 3890 4061 4082 5165 5222 5390 5984 9499
Size: 3733 171 21 1083 57 168 594 3515
Cuts at: 9499 157
Size: 3137
Fragments arranged by size:
3733 3515 3137 1083 594 171 168 57
21
AvrII C'CTAG_G
Cuts at: 2326 7828 2326
Size: 5502 6977
BamHI G'GATC_C
Cuts at: 848 1426 1545 3304 9504 848
Size: 578 119 1759 6200 3823
Fragments arranged by size:
6200 3823 1759 578 119
BanI G'GyrC_C
Cuts at: 1419 3065 4351 6794 7178 9495 9909 10157 10192
Size: 1646 1286 2443 384 2317 414 248 35
Cuts at: 10192 1419
Size: 3706
Fragments arranged by size:
3706 2443 2317 1646 1286 414 384 248
35
BanII G_rGCy'C
Cuts at: 2212 2807 4992 6281 6849 7286 7324 7724 9224
Size: 595 2185 1289 568 437 38 400 1500
Cuts at: 9224 9493 10523 2212
Size: 269 1030 4168
Fragments arranged by size:
4168 2185 1500 1289 1030 595 568 437
400 269 38
BbeI G_GCGC'C
Cuts at: 9913 10161 9913
Size: 248 12231
BbsI GAAGACnn'nnnn_
Cuts at: 2464 2519 4094 5684 6010 6089 6545 7457 2464
Size: 55 1575 1590 326 79 456 912 7486
Fragments arranged by size:
7486 1590 1575 912 456 326 79 55
BbvCI CC'TCA_GC
Cuts at: 1799 4982 1799
Size: 3183 9296
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 3182 3216 6376 6410 8385 8419 90
Size: 34 3058 34 3160 34 1975 34 4150
Fragments arranged by size:
4150 3160 3058 1975 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4462 10377 11138 12479 4462
Size: 5915 761 1341 4462
Fragments arranged by size:
5915 4462 1341 761
BclI T'GATC_A
Cuts at: 1062 3492 3929 7937 9999 1062
Size: 2430 437 4008 2062 3542
Fragments arranged by size:
4008 3542 2430 2062 437
BfrBI ATG'CAT
Cuts at: 3197 7153 3197
Size: 3956 8523
BglI GCCn_nnn'nGGC
Cuts at: 161 3557 161
Size: 3396 9083
BglII A'GATC_T
Cuts at: 3786 5946 9994 12031 3786
Size: 2160 4048 2037 4234
Fragments arranged by size:
4234 4048 2160 2037
BlpI GC'TnA_GC
Cuts at: 3677 9591 3677
Size: 5914 6565
Bme1580I G_kGCm'C
Cuts at: 2287 3108 3243 3511 4354 4638 4721 4843 4992
Size: 821 135 268 843 284 83 122 149
Cuts at: 4992 6878 7298 7917 8241 10104 10197 11253 2287
Size: 1886 420 619 324 1863 93 1056 3513
Fragments arranged by size:
3513 1886 1863 1056 843 821 619 420
324 284 268 149 135 122 93 83
BmgBI CAC'GTC
Cuts at: 3659 4204 6754 3659
Size: 545 2550 9384
Fragments arranged by size:
9384 2550 545
BmrI ACTGGGnnnn_n'
Cuts at: 440 674 3197 3509 3621 6827 6971 8332 9691
Size: 234 2523 312 112 3206 144 1361 1359
Cuts at: 9691 9772 10107 12251 440
Size: 81 335 2144 668
Fragments arranged by size:
3206 2523 2144 1361 1359 668 335 312
234 144 112 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3805 10846 3805
Size: 7041 5438
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 779 1116 1878 1954 2438 2702 3095 4208 4688
Size: 337 762 76 484 264 393 1113 480
Cuts at: 4688 4778 4894 6764 6887 7696 8261 8871 9050
Size: 90 116 1870 123 809 565 610 179
Cuts at: 9050 9430 779
Size: 380 3828
Fragments arranged by size:
3828 1870 1113 809 762 610 565 484
480 393 380 337 264 179 123 116
90 76
Bpu10I CC'TnA_GC
Cuts at: 1799 4982 7323 7703 8300 1799
Size: 3183 2341 380 597 5978
Fragments arranged by size:
5978 3183 2341 597 380
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2022 3850 3908 6165 6484 7563 9310 9655 10534
Size: 1828 58 2257 319 1079 1747 345 879
Cuts at: 10534 11026 11324 2022
Size: 492 298 3177
Fragments arranged by size:
3177 2257 1828 1747 1079 879 492 345
319 298 58
BsaI GGTCTCn'nnnn_
Cuts at: 765 7250 12183 765
Size: 6485 4933 1061
Fragments arranged by size:
6485 4933 1061
BsaAI yAC'GTr
Cuts at: 1213 4748 6991 9832 1213
Size: 3535 2243 2841 3860
Fragments arranged by size:
3860 3535 2841 2243
BsaBI GATnn'nnATC
Cuts at: 6 751 967 1810 7436 9993 12030 6
Size: 745 216 843 5626 2557 2037 455
Fragments arranged by size:
5626 2557 2037 843 745 455 216
BsaHI Gr'CG_yC
Cuts at: 2411 6895 9910 10158 10860 12356 2411
Size: 4484 3015 248 702 1496 2534
Fragments arranged by size:
4484 3015 2534 1496 702 248
BsaWI w'CCGG_w
Cuts at: 148 2582 3653 5098 8947 9668 10189 10867 11141
Size: 2434 1071 1445 3849 721 521 678 274
Cuts at: 11141 11288 11699 148
Size: 147 411 928
Fragments arranged by size:
3849 2434 1445 1071 928 721 678 521
411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 762 792 1220 1250 1952 1982 2819 2849 4203
Size: 30 428 30 702 30 837 30 1354
Cuts at: 4203 4233 6885 6915 762
Size: 30 2652 30 6326
Fragments arranged by size:
6326 2652 1354 837 702 428 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 121 1604 2295 3977 4211 5307 5725 6643 6890
Size: 1483 691 1682 234 1096 418 918 247
Cuts at: 6890 7459 7864 121
Size: 569 405 4736
Fragments arranged by size:
4736 1682 1483 1096 918 691 569 418
405 247 234
BseYI C'CCAG_C
Cuts at: 1775 1893 2965 4811 6877 8316 9148 9264 9465
Size: 118 1072 1846 2066 1439 832 116 201
Cuts at: 9465 9904 10662 11239 11735 1775
Size: 439 758 577 496 2519
Fragments arranged by size:
2519 2066 1846 1439 1072 832 758 577
496 439 201 118 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 687 2020 2669 2943 3123 3405 4572 4728 687
Size: 1333 649 274 180 282 1167 156 8438
Fragments arranged by size:
8438 1333 1167 649 282 274 180 156
BsiEI CG_ry'CG
Cuts at: 104 181 10021 10067 11275 104
Size: 77 9840 46 1208 1308
Fragments arranged by size:
9840 1308 1208 77 46
BsiHKAI G_wGCw'C
Cuts at: 2212 2807 3243 3511 3634 4464 4843 6281 7233
Size: 595 436 268 123 830 379 1438 952
Cuts at: 7233 7286 7724 8103 9224 9493 10271 10461 11253
Size: 53 438 379 1121 269 778 190 792
Cuts at: 11253 2212
Size: 3438
Fragments arranged by size:
3438 1438 1121 952 830 792 778 595
438 436 379 379 269 268 190 123
53
BsmI GAATG_Cn'
Cuts at: 867 1168 1760 2428 6351 6569 8762 867
Size: 301 592 668 3923 218 2193 4584
Fragments arranged by size:
4584 3923 2193 668 592 301 218
BsmBI CGTCTCn'nnnn_
Cuts at: 5219 6902 8470 5219
Size: 1683 1568 9228
Fragments arranged by size:
9228 1683 1568
Bsp1286I G_dGCh'C
Cuts at: 2212 2287 2807 3108 3243 3511 3634 4354 4464
Size: 75 520 301 135 268 123 720 110
Cuts at: 4464 4638 4721 4843 4992 6281 6849 6878 7233
Size: 174 83 122 149 1289 568 29 355
Cuts at: 7233 7286 7298 7324 7724 7917 8103 8241 9224
Size: 53 12 26 400 193 186 138 983
Cuts at: 9224 9493 10104 10197 10271 10461 10523 11253 2212
Size: 269 611 93 74 190 62 730 3438
Fragments arranged by size:
3438 1289 983 730 720 611 568 520
400 355 301 269 268 193 190 186
174 149 138 135 123 122 110 93
83 75 74 62 53 29 26 12
BspEI T'CCGG_A
Cuts at: 148 3653 11699 148
Size: 3505 8046 928
Fragments arranged by size:
8046 3505 928
BspHI T'CATG_A
Cuts at: 4886 7271 7962 11655 4886
Size: 2385 691 3693 5710
Fragments arranged by size:
5710 3693 2385 691
BspMI ACCTGCnnnn'nnnn_
Cuts at: 492 2042 9529 9743 10045 10426 492
Size: 1550 7487 214 302 381 2545
Fragments arranged by size:
7487 2545 1550 381 302 214
BsrBI CCG'CTC
Cuts at: 50 820 10771 10825 12309 12474 50
Size: 770 9951 54 1484 165 55
Fragments arranged by size:
9951 1484 770 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1350 1434 2255 2715 3330 4516 5619 6380 6944
Size: 84 821 460 615 1186 1103 761 564
Cuts at: 6944 8426 10391 1350
Size: 1482 1965 3438
Fragments arranged by size:
3438 1965 1482 1186 1103 821 761 615
564 460 84
BsrFI r'CCGG_y
Cuts at: 1866 2792 2872 3551 6428 8947 10477 10658 10867
Size: 926 80 679 2877 2519 1530 181 209
Cuts at: 10867 1866
Size: 3478
Fragments arranged by size:
3478 2877 2519 1530 926 679 209 181
80
BsrGI T'GTAC_A
Cuts at: 3376 3376
Size: 12479
BssHII G'CGCG_C
Cuts at: 1862 1862
Size: 12479
BssSI C'ACGA_G
Cuts at: 5035 7253 10750 11108 5035
Size: 2218 3497 358 6406
Fragments arranged by size:
6406 3497 2218 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1839 2839 3602 7088 9856 1839
Size: 1000 763 3486 2768 4462
Fragments arranged by size:
4462 3486 2768 1000 763
BstBI TT'CG_AA
Cuts at: 6573 10840 6573
Size: 4267 8212
BstEII G'GTnAC_C
Cuts at: 8774 8774
Size: 12479
BstXI CCAn_nnnn'nTGG
Cuts at: 6094 7363 9432 6094
Size: 1269 2069 9141
Fragments arranged by size:
9141 2069 1269
BstYI r'GATC_y
Cuts at: 1 848 1426 1545 3304 3786 5418 5946 6138
Size: 847 578 119 1759 482 1632 528 192
Cuts at: 6138 6360 6739 9373 9504 9971 9994 10329 10575
Size: 222 379 2634 131 467 23 335 246
Cuts at: 10575 11587 11673 11685 12031 1
Size: 1012 86 12 346 449
Fragments arranged by size:
2634 1759 1632 1012 847 578 528 482
467 449 379 346 335 246 222 192
131 119 86 23 12
BstZ17I GTA'TAC
Cuts at: 2297 2297
Size: 12479
Bsu36I CC'TnA_GG
Cuts at: 7103 7103
Size: 12479
BtgI C'CryG_G
Cuts at: 3108 4600 4763 8186 3108
Size: 1492 163 3423 7401
Fragments arranged by size:
7401 3423 1492 163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1667 3207 10429 10610 10773 1667
Size: 1540 7222 181 163 3373
Fragments arranged by size:
7222 3373 1540 181 163
BtsI GCAGTG_nn'
Cuts at: 358 1259 1527 3511 4433 4708 5870 9131 9191
Size: 901 268 1984 922 275 1162 3261 60
Cuts at: 9191 9722 358
Size: 531 3115
Fragments arranged by size:
3261 3115 1984 1162 922 901 531 275
268 60
ClaI AT'CG_AT
Cuts at: 4408 4408
Size: 12479
DraI TTT'AAA
Cuts at: 5603 9478 5603
Size: 3875 8604
DraIII CAC_nnn'GTG
Cuts at: 434 632 2638 434
Size: 198 2006 10275
Fragments arranged by size:
10275 2006 198
DrdI GACnn_nn'nnGTC
Cuts at: 913 10185 11043 913
Size: 9272 858 2349
Fragments arranged by size:
9272 2349 858
EaeI y'GGCC_r
Cuts at: 178 1755 3996 4326 8640 10064 10238 10629 10656
Size: 1577 2241 330 4314 1424 174 391 27
Cuts at: 10656 12001 178
Size: 1345 656
Fragments arranged by size:
4314 2241 1577 1424 1345 656 391 330
174 27
EagI C'GGCC_G
Cuts at: 178 10064 178
Size: 9886 2593
EarI CTCTTCn'nnn_
Cuts at: 1937 2146 3041 7238 7965 10502 10712 1937
Size: 209 895 4197 727 2537 210 3704
Fragments arranged by size:
4197 3704 2537 895 727 210 209
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 10687 10997 11143 12289 10687
Size: 310 146 1146 10877
Fragments arranged by size:
10877 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2210 2805 6279 7284 7722 9222 9491 2210
Size: 595 3474 1005 438 1500 269 5198
Fragments arranged by size:
5198 3474 1500 1005 595 438 269
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 779 1116 1178 1284 1878 1954 2066 2184 2438
Size: 337 62 106 594 76 112 118 254
Cuts at: 2438 2531 2702 3095 3656 3691 4208 4688 4778
Size: 93 171 393 561 35 517 480 90
Cuts at: 4778 4894 5242 5348 6004 6015 6322 6539 6764
Size: 116 348 106 656 11 307 217 225
Cuts at: 6764 6887 7594 7696 8261 8565 8871 9050 9400
Size: 123 707 102 565 304 306 179 350
Cuts at: 9400 9430 10304 10736 11483 11751 779
Size: 30 874 432 747 268 1507
Fragments arranged by size:
1507 874 747 707 656 594 565 561
517 480 432 393 350 348 337 307
306 304 268 254 225 217 179 171
123 118 116 112 106 106 102 93
90 76 62 35 30 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2498 4733 5842 8147 2498
Size: 2235 1109 2305 6830
Fragments arranged by size:
6830 2305 2235 1109
EcoO109I rG'GnC_Cy
Cuts at: 126 292 2969 4989 5469 5980 8859 9429 9618
Size: 166 2677 2020 480 511 2879 570 189
Cuts at: 9618 126
Size: 2987
Fragments arranged by size:
2987 2879 2677 2020 570 511 480 189
166
EcoRI G'AATT_C
Cuts at: 5146 7394 8684 9007 9483 5146
Size: 2248 1290 323 476 8142
Fragments arranged by size:
8142 2248 1290 476 323
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6034 8704 10498 6034
Size: 2670 1794 8015
Fragments arranged by size:
8015 2670 1794
FspI TGC'GCA
Cuts at: 10260 10260
Size: 12479
HaeII r_GCGC'y
Cuts at: 9913 10161 11183 12162 9913
Size: 248 1022 979 10230
Fragments arranged by size:
10230 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 867 899 1220 1252 1452 1484 1777 1809 2600
Size: 32 321 32 200 32 293 32 791
Cuts at: 2600 2632 2737 2769 3771 3803 3936 3968 5286
Size: 32 105 32 1002 32 133 32 1318
Cuts at: 5286 5318 5722 5754 5761 5793 6724 6756 6885
Size: 32 404 32 7 32 931 32 129
Cuts at: 6885 6917 7079 7111 7306 7338 8337 8369 9036
Size: 32 162 32 195 32 999 32 667
Cuts at: 9036 9068 9353 9385 10321 10351 10353 10383 867
Size: 32 285 32 936 30 2 30 2963
Fragments arranged by size:
2963 1318 1002 999 936 931 791 667
404 321 293 285 200 195 162 133
129 105 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 30 30 7 2
HincII GTy'rAC
Cuts at: 1913 2217 2343 2521 4426 6624 9518 1913
Size: 304 126 178 1905 2198 2894 4874
Fragments arranged by size:
4874 2894 2198 1905 304 178 126
HindIII A'AGCT_T
Cuts at: 5761 5885 6027 8083 5761
Size: 124 142 2056 10157
Fragments arranged by size:
10157 2056 142 124
HpaI GTT'AAC
Cuts at: 2217 2343 2217
Size: 126 12353
KasI G'GCGC_C
Cuts at: 9909 10157 9909
Size: 248 12231
KpnI G_GTAC'C
Cuts at: 6798 7182 9499 6798
Size: 384 2317 9778
Fragments arranged by size:
9778 2317 384
MfeI C'AATT_G
Cuts at: 1140 1361 4000 7418 7736 8432 8471 1140
Size: 221 2639 3418 318 696 39 5148
Fragments arranged by size:
5148 3418 2639 696 318 221 39
MluI A'CGCG_T
Cuts at: 11912 11912
Size: 12479
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 936 6717 6729 8459 8732 9285 11150 11334 936
Size: 5781 12 1730 273 553 1865 184 2081
Fragments arranged by size:
5781 2081 1865 1730 553 273 184 12
MscI TGG'CCA
Cuts at: 3998 8642 10240 3998
Size: 4644 1598 6237
Fragments arranged by size:
6237 4644 1598
MslI CAynn'nnrTG
Cuts at: 1765 2057 2379 2447 2703 4368 4410 4796 6092
Size: 292 322 68 256 1665 42 386 1296
Cuts at: 6092 7198 7276 7361 9167 10468 10595 1765
Size: 1106 78 85 1806 1301 127 3649
Fragments arranged by size:
3649 1806 1665 1301 1296 1106 386 322
292 256 127 85 78 68 42
MspA1I CmG'CkG
Cuts at: 177 192 2230 2266 2741 3101 3602 4228 4586
Size: 15 2038 36 475 360 501 626 358
Cuts at: 4586 4911 4986 5010 6199 6637 8281 8559 9280
Size: 325 75 24 1189 438 1644 278 721
Cuts at: 9280 9590 9904 10264 11277 11522 177
Size: 310 314 360 1013 245 1134
Fragments arranged by size:
2038 1644 1189 1134 1013 721 626 501
475 438 360 360 358 325 314 310
278 245 75 36 24 15
NaeI GCC'GGC
Cuts at: 1868 3553 10660 1868
Size: 1685 7107 3687
Fragments arranged by size:
7107 3687 1685
NarI GG'CG_CC
Cuts at: 9910 10158 9910
Size: 248 12231
NcoI C'CATG_G
Cuts at: 8186 8186
Size: 12479
NdeI CA'TA_TG
Cuts at: 678 1929 3977 8158 678
Size: 1251 2048 4181 4999
Fragments arranged by size:
4999 4181 2048 1251
NgoMIV G'CCGG_C
Cuts at: 1866 3551 10658 1866
Size: 1685 7107 3687
Fragments arranged by size:
7107 3687 1685
NotI GC'GGCC_GC
Cuts at: 178 178
Size: 12479
NsiI A_TGCA'T
Cuts at: 3199 7155 3199
Size: 3956 8523
NspI r_CATG'y
Cuts at: 281 325 1049 1139 1286 1405 4289 4801 8046
Size: 44 724 90 147 119 2884 512 3245
Cuts at: 8046 8909 9532 10032 10939 281
Size: 863 623 500 907 1821
Fragments arranged by size:
3245 2884 1821 907 863 724 623 512
500 147 119 90 44
PasI CC'CwG_GG
Cuts at: 328 3439 4257 4347 5139 328
Size: 3111 818 90 792 7668
Fragments arranged by size:
7668 3111 818 792 90
PciI A'CATG_T
Cuts at: 277 321 1045 1282 8905 10935 277
Size: 44 724 237 7623 2030 1821
Fragments arranged by size:
7623 2030 1821 724 237 44
PflMI CCAn_nnn'nTGG
Cuts at: 3187 3251 7175 3187
Size: 64 3924 8491
Fragments arranged by size:
8491 3924 64
PfoI T'CCnGG_A
Cuts at: 1074 5423 8741 1074
Size: 4349 3318 4812
Fragments arranged by size:
4812 4349 3318
PmeI GTTT'AAAC
Cuts at: 9478 9478
Size: 12479
PmlI CAC'GTG
Cuts at: 4748 4748
Size: 12479
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1952 1984 2918 2950 11618 11650 1952
Size: 32 934 32 8668 32 2781
Fragments arranged by size:
8668 2781 934 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 126 292 2969 5469 5980 126
Size: 166 2677 2500 511 6625
Fragments arranged by size:
6625 2677 2500 511 166
PshAI GACnn'nnGTC
Cuts at: 1598 2585 8228 1598
Size: 987 5643 5849
Fragments arranged by size:
5849 5643 987
PspOMI G'GGCC_C
Cuts at: 4988 4988
Size: 12479
PspXI vC'TCGA_Gb
Cuts at: 4061 4061
Size: 12479
PstI C_TGCA'G
Cuts at: 1522 3295 3598 3716 5349 5877 8367 9526 10211
Size: 1773 303 118 1633 528 2490 1159 685
Cuts at: 10211 1522
Size: 3790
Fragments arranged by size:
3790 2490 1773 1633 1159 685 528 303
118
PvuI CG_AT'CG
Cuts at: 104 104
Size: 12479
PvuII CAG'CTG
Cuts at: 2266 2741 3602 4228 5010 6199 6637 8281 8559
Size: 475 861 626 782 1189 438 1644 278
Cuts at: 8559 9280 9904 10264 2266
Size: 721 624 360 4481
Fragments arranged by size:
4481 1644 1189 861 782 721 626 624
475 438 360 278
RsrII CG'GwC_CG
Cuts at: 10674 10674
Size: 12479
SacI G_AGCT'C
Cuts at: 2212 2807 6281 7286 7724 9224 9493 2212
Size: 595 3474 1005 438 1500 269 5198
Fragments arranged by size:
5198 3474 1500 1005 595 438 269
SalI G'TCGA_C
Cuts at: 9516 9516
Size: 12479
SapI GCTCTTCn'nnn_
Cuts at: 7238 10502 10712 7238
Size: 3264 210 9005
Fragments arranged by size:
9005 3264 210
SbfI CC_TGCA'GG
Cuts at: 9526 9526
Size: 12479
ScaI AGT'ACT
Cuts at: 4621 11996 4621
Size: 7375 5104
SexAI A'CCwGG_T
Cuts at: 2778 4320 9239 2778
Size: 1542 4919 6018
Fragments arranged by size:
6018 4919 1542
SfcI C'TryA_G
Cuts at: 33 1518 1596 2267 3291 3486 3594 3712 5345
Size: 1485 78 671 1024 195 108 118 1633
Cuts at: 5345 5873 6695 7043 8363 8817 9522 10207 11200
Size: 528 822 348 1320 454 705 685 993
Cuts at: 11200 11391 33
Size: 191 1121
Fragments arranged by size:
1633 1485 1320 1121 1024 993 822 705
685 671 528 454 348 195 191 118
108 78
SfoI GGC'GCC
Cuts at: 9911 10159 9911
Size: 248 12231
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 12479
SmaI CCC'GGG
Cuts at: 5167 5392 9501 5167
Size: 225 4109 8145
Fragments arranged by size:
8145 4109 225
SmlI C'TyrA_G
Cuts at: 2001 3865 3923 4061 5222 6144 6463 7542 9325
Size: 1864 58 138 1161 922 319 1079 1783
Cuts at: 9325 9634 10549 11041 11303 2001
Size: 309 915 492 262 3177
Fragments arranged by size:
3177 1864 1783 1161 1079 922 915 492
319 309 262 138 58
SpeI A'CTAG_T
Cuts at: 67 67
Size: 12479
SphI G_CATG'C
Cuts at: 1139 4289 4801 9532 10032 1139
Size: 3150 512 4731 500 3586
Fragments arranged by size:
4731 3586 3150 512 500
SspI AAT'ATT
Cuts at: 651 4045 5898 6615 7582 8136 651
Size: 3394 1853 717 967 554 4994
Fragments arranged by size:
4994 3394 1853 967 717 554
StuI AGG'CCT
Cuts at: 155 5233 7858 8202 155
Size: 5078 2625 344 4432
Fragments arranged by size:
5078 4432 2625 344
StyI C'CwwG_G
Cuts at: 1330 2326 3308 3728 7828 8186 9425 9613 9967
Size: 996 982 420 4100 358 1239 188 354
Cuts at: 9967 1330
Size: 3842
Fragments arranged by size:
4100 3842 1239 996 982 420 358 354
188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 927 2054 3258 3651 5056 8623 10062 10195 10654
Size: 1127 1204 393 1405 3567 1439 133 459
Cuts at: 10654 10863 927
Size: 209 2543
Fragments arranged by size:
3567 2543 1439 1405 1204 1127 459 393
209 133
TatI w'GTAC_w
Cuts at: 302 1220 1285 3376 4619 6258 11994 302
Size: 918 65 2091 1243 1639 5736 787
Fragments arranged by size:
5736 2091 1639 1243 918 787 65
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 762 794 2091 2123 2528 2560 3078 3110 4344
Size: 32 1297 32 405 32 518 32 1234
Cuts at: 4344 4376 4581 4613 7083 7115 8963 8995 11996
Size: 32 205 32 2470 32 1848 32 3001
Cuts at: 11996 12028 762
Size: 32 1213
Fragments arranged by size:
3001 2470 1848 1297 1234 1213 518 405
205 32 32 32 32 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 4729 6331 10276 4729
Size: 1602 3945 6932
Fragments arranged by size:
6932 3945 1602
XbaI T'CTAG_A
Cuts at: 5304 6918 9510 5304
Size: 1614 2592 8273
Fragments arranged by size:
8273 2592 1614
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1261 2905 3693 6292 9974 1261
Size: 1644 788 2599 3682 3766
Fragments arranged by size:
3766 3682 2599 1644 788
XhoI C'TCGA_G
Cuts at: 4061 5222 4061
Size: 1161 11318
XmaI C'CCGG_G
Cuts at: 5165 5390 9499 5165
Size: 225 4109 8145
Fragments arranged by size:
8145 4109 225
XmnI GAAnn'nnTTC
Cuts at: 1964 5255 5354 6028 6834 7218 7332 7378 8684
Size: 3291 99 674 806 384 114 46 1306
Cuts at: 8684 1964
Size: 5759
Fragments arranged by size:
5759 3291 1306 806 674 384 114 99
46
ZraI GAC'GTC
Cuts at: 2412 6896 2412
Size: 4484 7995
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AscI BaeI BmtI BsiWI CspCI EcoRV
FseI FspAI NheI NruI PacI PsiI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SwaI