Restriction map for
pF1KE2532
(Circular) (Six-base) MAPSORT of: pF1KE2532.seq Check: 1342 from: 1 to: 8894
pF1KE2532 8894 bp
With 182 enzymes: SgfI *
August 6, 2014 11:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2209 2209
Size: 8894
AatII G_ACGT'C
Cuts at: 1427 3716 1427
Size: 2289 6605
AccI GT'mk_AC
Cuts at: 2423 5932 2423
Size: 3509 5385
Acc65I G'GTAC_C
Cuts at: 5910 5910
Size: 8894
AclI AA'CG_TT
Cuts at: 8734 8734
Size: 8894
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 318 466 606 797 984 1111 1277 2556 5277
Size: 148 140 191 187 127 166 1279 2721
Cuts at: 5277 5293 6719 7151 7898 8166 318
Size: 16 1426 432 747 268 1046
Fragments arranged by size:
2721 1426 1279 1046 747 432 268 191
187 166 148 140 127 16
AfeI AGC'GCT
Cuts at: 1972 5830 1972
Size: 3858 5036
AflII C'TTAA_G
Cuts at: 2415 2415
Size: 8894
AflIII A'CryG_T
Cuts at: 287 2241 3425 3728 4328 6246 7350 8327 287
Size: 1954 1184 303 600 1918 1104 977 854
Fragments arranged by size:
1954 1918 1184 1104 977 854 600 303
AgeI A'CCGG_T
Cuts at: 7282 7282
Size: 8894
AhdI GACnn_n'nnGTC
Cuts at: 174 174
Size: 8894
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4749 4781 5698 5730 4749
Size: 32 917 32 7913
Fragments arranged by size:
7913 917 32 32
AlwNI CAG_nnn'CTG
Cuts at: 309 897 1617 2127 2900 4267 4431 4683 4910
Size: 588 720 510 773 1367 164 252 227
Cuts at: 4910 5257 5718 6199 7766 309
Size: 347 461 481 1567 1437
Fragments arranged by size:
1567 1437 1367 773 720 588 510 481
461 347 252 227 164
ApaLI G'TGCA_C
Cuts at: 7664 7664
Size: 8894
ApoI r'AATT_y
Cuts at: 186 3340 5092 5898 8247 8503 186
Size: 3154 1752 806 2349 256 577
Fragments arranged by size:
3154 2349 1752 806 577 256
AseI AT'TA_AT
Cuts at: 20 1055 20
Size: 1035 7859
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8894
AvaI C'yCGr_G
Cuts at: 2098 5914 2098
Size: 3816 5078
BamHI G'GATC_C
Cuts at: 1341 5042 5300 5573 5919 1341
Size: 3701 258 273 346 4316
Fragments arranged by size:
4316 3701 346 273 258
BanI G'GyrC_C
Cuts at: 1273 1762 1853 2289 2733 3372 5125 5734 5910
Size: 489 91 436 444 639 1753 609 176
Cuts at: 5910 6324 6572 6607 1273
Size: 414 248 35 3560
Fragments arranged by size:
3560 1753 639 609 489 444 436 414
248 176 91 35
BanII G_rGCy'C
Cuts at: 794 1452 1580 3305 3500 4724 5875 5908 6938
Size: 658 128 1725 195 1224 1151 33 1030
Cuts at: 6938 794
Size: 2750
Fragments arranged by size:
2750 1725 1224 1151 1030 658 195 128
33
BbeI G_GCGC'C
Cuts at: 6328 6576 6328
Size: 248 8646
BbsI GAAGACnn'nnnn_
Cuts at: 2477 3708 3794 5041 2477
Size: 1231 86 1247 6330
Fragments arranged by size:
6330 1247 1231 86
BbvCI CC'TCA_GC
Cuts at: 3806 4406 4835 3806
Size: 600 429 7865
Fragments arranged by size:
7865 600 429
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 529 563 718 752 5225 5259 529
Size: 34 155 34 4473 34 4164
Fragments arranged by size:
4473 4164 155 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1239 2334 3954 5464 6792 7553 8894 1239
Size: 1095 1620 1510 1328 761 1341 1239
Fragments arranged by size:
1620 1510 1341 1328 1239 1095 761
BclI T'GATC_A
Cuts at: 641 3116 6414 641
Size: 2475 3298 3121
Fragments arranged by size:
3298 3121 2475
BglII A'GATC_T
Cuts at: 6409 8446 6409
Size: 2037 6857
BlpI GC'TnA_GC
Cuts at: 3472 6006 3472
Size: 2534 6360
Bme1580I G_kGCm'C
Cuts at: 390 1502 1895 2294 2738 3375 6519 6612 7668
Size: 1112 393 399 444 637 3144 93 1056
Cuts at: 7668 390
Size: 1616
Fragments arranged by size:
3144 1616 1112 1056 637 444 399 393
93
BmgBI CAC'GTC
Cuts at: 2565 4377 2565
Size: 1812 7082
BmrI ACTGGGnnnn_n'
Cuts at: 180 1608 1929 3074 3498 4596 4920 6106 6187
Size: 1428 321 1145 424 1098 324 1186 81
Cuts at: 6187 6522 8666 180
Size: 335 2144 408
Fragments arranged by size:
2144 1428 1186 1145 1098 424 408 335
324 321 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2754 7261 2754
Size: 4507 4387
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 1405 1922 2754 2972 3172 3723 4275 4751
Size: 738 517 832 218 200 551 552 476
Cuts at: 4751 667
Size: 4810
Fragments arranged by size:
4810 832 738 552 551 517 476 218
200
Bpu10I CC'TnA_GC
Cuts at: 1451 3333 3806 4406 4835 1451
Size: 1882 473 600 429 5510
Fragments arranged by size:
5510 1882 600 473 429
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 451 1816 2440 6070 6949 7441 7739 442
Size: 9 1365 624 3630 879 492 298 1597
Fragments arranged by size:
3630 1597 1365 879 624 492 298 9
BsaI GGTCTCn'nnnn_
Cuts at: 245 247 1147 3053 4061 4472 4627 8598 245
Size: 2 900 1906 1008 411 155 3971 541
Fragments arranged by size:
3971 1906 1008 900 541 411 155 2
BsaAI yAC'GTr
Cuts at: 1896 2244 3428 3731 4527 6247 1896
Size: 348 1184 303 796 1720 4543
Fragments arranged by size:
4543 1720 1184 796 348 303
BsaBI GATnn'nnATC
Cuts at: 6 2990 4891 6408 8445 6
Size: 2984 1901 1517 2037 455
Fragments arranged by size:
2984 2037 1901 1517 455
BsaHI Gr'CG_yC
Cuts at: 1424 3713 5281 6325 6573 7275 8771 1424
Size: 2289 1568 1044 248 702 1496 1547
Fragments arranged by size:
2289 1568 1547 1496 1044 702 248
BsaWI w'CCGG_w
Cuts at: 530 6083 6604 7282 7556 7703 8114 530
Size: 5553 521 678 274 147 411 1310
Fragments arranged by size:
5553 1310 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 380 410 952 982 1450 1480 2683 2713 3763
Size: 30 542 30 468 30 1203 30 1050
Cuts at: 3763 3793 4204 4234 4589 4619 4679 4709 4749
Size: 30 411 30 355 30 60 30 40
Cuts at: 4749 4779 5245 5275 5698 5728 380
Size: 30 466 30 423 30 3546
Fragments arranged by size:
3546 1203 1050 542 468 466 423 411
355 60 40 30 30 30 30 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 385 445 1155 3823 3826 5127 5234 5719 385
Size: 60 710 2668 3 1301 107 485 3560
Fragments arranged by size:
3560 2668 1301 710 485 107 60 3
BseYI C'CCAG_C
Cuts at: 158 399 984 1307 1513 1989 2031 2893 3203
Size: 241 585 323 206 476 42 862 310
Cuts at: 3203 3368 4622 4683 5366 6319 7077 7654 8150
Size: 165 1254 61 683 953 758 577 496
Cuts at: 8150 158
Size: 902
Fragments arranged by size:
1254 953 902 862 758 683 585 577
496 476 323 310 241 206 165 61
42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 457 458 484 2542 3769 4280 4663 5467
Size: 246 1 26 2058 1227 511 383 804
Cuts at: 5467 5731 211
Size: 264 3374
Fragments arranged by size:
3374 2058 1227 804 511 383 264 246
26 1
BsiEI CG_ry'CG
Cuts at: 104 836 3710 6436 6482 7690 104
Size: 732 2874 2726 46 1208 1308
Fragments arranged by size:
2874 2726 1308 1208 732 46
BsiHKAI G_wGCw'C
Cuts at: 794 1037 2077 2456 4673 5908 6686 6876 7668
Size: 243 1040 379 2217 1235 778 190 792
Cuts at: 7668 794
Size: 2020
Fragments arranged by size:
2217 2020 1235 1040 792 778 379 243
190
BsiWI C'GTAC_G
Cuts at: 3428 3428
Size: 8894
BsmI GAATG_Cn'
Cuts at: 871 3548 871
Size: 2677 6217
BsmBI CGTCTCn'nnnn_
Cuts at: 4367 4367
Size: 8894
Bsp1286I G_dGCh'C
Cuts at: 390 794 1037 1452 1502 1580 1895 2077 2294
Size: 404 243 415 50 78 315 182 217
Cuts at: 2294 2456 2738 3305 3375 3500 4673 4724 5875
Size: 162 282 567 70 125 1173 51 1151
Cuts at: 5875 5908 6519 6612 6686 6876 6938 7668 390
Size: 33 611 93 74 190 62 730 1616
Fragments arranged by size:
1616 1173 1151 730 611 567 415 404
315 282 243 217 190 182 162 125
93 78 74 70 62 51 50 33
BspEI T'CCGG_A
Cuts at: 530 8114 530
Size: 7584 1310
BspHI T'CATG_A
Cuts at: 8070 8070
Size: 8894
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1613 2209 3182 5944 6158 6460 6841 1613
Size: 596 973 2762 214 302 381 3666
Fragments arranged by size:
3666 2762 973 596 381 302 214
BsrBI CCG'CTC
Cuts at: 50 7186 7240 8724 8889 50
Size: 7136 54 1484 165 55
Fragments arranged by size:
7136 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2495 2937 3845 4730 5625 6806 2495
Size: 442 908 885 895 1181 4583
Fragments arranged by size:
4583 1181 908 895 885 442
BsrFI r'CCGG_y
Cuts at: 446 1765 6892 7073 7282 446
Size: 1319 5127 181 209 2058
Fragments arranged by size:
5127 2058 1319 209 181
BsrGI T'GTAC_A
Cuts at: 1457 4850 1457
Size: 3393 5501
BssSI C'ACGA_G
Cuts at: 1150 5244 7165 7523 1150
Size: 4094 1921 358 2521
Fragments arranged by size:
4094 2521 1921 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1986 4267 6271 1986
Size: 2281 2004 4609
Fragments arranged by size:
4609 2281 2004
BstBI TT'CG_AA
Cuts at: 2522 2786 7255 2522
Size: 264 4469 4161
Fragments arranged by size:
4469 4161 264
BstEII G'GTnAC_C
Cuts at: 1719 4416 4572 1719
Size: 2697 156 6041
Fragments arranged by size:
6041 2697 156
BstXI CCAn_nnnn'nTGG
Cuts at: 2551 3265 3312 3525 4747 2551
Size: 714 47 213 1222 6698
Fragments arranged by size:
6698 1222 714 213 47
BstYI r'GATC_y
Cuts at: 1 608 1341 1484 2051 4410 4926 5042 5300
Size: 607 733 143 567 2359 516 116 258
Cuts at: 5300 5402 5573 5919 6386 6409 6744 6990 8002
Size: 102 171 346 467 23 335 246 1012
Cuts at: 8002 8088 8100 8446 1
Size: 86 12 346 449
Fragments arranged by size:
2359 1012 733 607 567 516 467 449
346 346 335 258 246 171 143 116
102 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1610 1949 2041 4241 1610
Size: 339 92 2200 6263
Fragments arranged by size:
6263 2200 339 92
BtgI C'CryG_G
Cuts at: 107 488 1157 1493 1580 1735 2805 3149 3833
Size: 381 669 336 87 155 1070 344 684
Cuts at: 3833 107
Size: 5168
Fragments arranged by size:
5168 1070 684 669 381 344 336 155
87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1858 2943 5217 5503 6844 7025 7188 1858
Size: 1085 2274 286 1341 181 163 3564
Fragments arranged by size:
3564 2274 1341 1085 286 181 163
BtsI GCAGTG_nn'
Cuts at: 307 445 4543 4790 4911 5189 5393 6137 307
Size: 138 4098 247 121 278 204 744 3064
Fragments arranged by size:
4098 3064 744 278 247 204 138 121
ClaI AT'CG_AT
Cuts at: 4538 4538
Size: 8894
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 219 254 219
Size: 35 8859
DraI TTT'AAA
Cuts at: 5893 5893
Size: 8894
DrdI GACnn_nn'nnGTC
Cuts at: 6600 7458 6600
Size: 858 8036
EaeI y'GGCC_r
Cuts at: 1391 1583 1767 3707 4205 4550 4809 5248 6479
Size: 192 184 1940 498 345 259 439 1231
Cuts at: 6479 6653 7044 7071 8416 1391
Size: 174 391 27 1345 1869
Fragments arranged by size:
1940 1869 1345 1231 498 439 391 345
259 192 184 174 27
EagI C'GGCC_G
Cuts at: 3707 6479 3707
Size: 2772 6122
EarI CTCTTCn'nnn_
Cuts at: 562 1003 2850 3677 3682 3887 4678 5104 5205
Size: 441 1847 827 5 205 791 426 101
Cuts at: 5205 6917 7127 562
Size: 1712 210 2329
Fragments arranged by size:
2329 1847 1712 827 791 441 426 210
205 101 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 764 1237 4149 7102 7412 7558 8704 764
Size: 473 2912 2953 310 146 1146 954
Fragments arranged by size:
2953 2912 1146 954 473 310 146
EcoICRI GAG'CTC
Cuts at: 792 5906 792
Size: 5114 3780
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 318 466 606 667 797 984 1111 1277 1405
Size: 148 140 61 130 187 127 166 128
Cuts at: 1405 1922 2556 2754 2972 3172 3723 4275 4751
Size: 517 634 198 218 200 551 552 476
Cuts at: 4751 5277 5293 6719 7151 7898 8166 318
Size: 526 16 1426 432 747 268 1046
Fragments arranged by size:
1426 1046 747 634 552 551 526 517
476 432 268 218 200 198 187 166
148 140 130 128 127 61 16
EcoNI CCTnn'n_nnAGG
Cuts at: 1609 2653 3178 4161 1609
Size: 1044 525 983 6342
Fragments arranged by size:
6342 1044 983 525
EcoO109I rG'GnC_Cy
Cuts at: 462 597 1352 1602 1614 1953 2045 2527 2890
Size: 135 755 250 12 339 92 482 363
Cuts at: 2890 3171 3183 3360 3513 3816 4590 5644 6033
Size: 281 12 177 153 303 774 1054 389
Cuts at: 6033 462
Size: 3323
Fragments arranged by size:
3323 1054 774 755 482 389 363 339
303 281 250 177 153 135 92 12
12
EcoRI G'AATT_C
Cuts at: 5898 5898
Size: 8894
EcoRV GAT'ATC
Cuts at: 783 3195 783
Size: 2412 6482
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 755 1115 6913 755
Size: 360 5798 2736
Fragments arranged by size:
5798 2736 360
FspI TGC'GCA
Cuts at: 6675 6675
Size: 8894
HaeII r_GCGC'y
Cuts at: 1974 5832 6328 6576 7598 8577 1974
Size: 3858 496 248 1022 979 2291
Fragments arranged by size:
3858 2291 1022 979 496 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 160 192 829 861 952 984 3487 3519 4031
Size: 32 637 32 91 32 2503 32 512
Cuts at: 4031 4063 5245 5277 5517 5549 5669 5701 6736
Size: 32 1182 32 240 32 120 32 1035
Cuts at: 6736 6766 6768 6798 160
Size: 30 2 30 2256
Fragments arranged by size:
2503 2256 1182 1035 637 512 240 120
91 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2568 5933 2568
Size: 3365 5529
HindIII A'AGCT_T
Cuts at: 3669 3669
Size: 8894
KasI G'GCGC_C
Cuts at: 6324 6572 6324
Size: 248 8646
KpnI G_GTAC'C
Cuts at: 5914 5914
Size: 8894
MluI A'CGCG_T
Cuts at: 8327 8327
Size: 8894
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2079 7565 7749 2079
Size: 5486 184 3224
Fragments arranged by size:
5486 3224 184
MscI TGG'CCA
Cuts at: 1393 1585 4207 4811 5250 6655 1393
Size: 192 2622 604 439 1405 3632
Fragments arranged by size:
3632 2622 1405 604 439 192
MslI CAynn'nnrTG
Cuts at: 3838 6883 7010 3838
Size: 3045 127 5722
Fragments arranged by size:
5722 3045 127
MspA1I CmG'CkG
Cuts at: 202 2547 2982 3535 4647 5370 6005 6319 6679
Size: 2345 435 553 1112 723 635 314 360
Cuts at: 6679 7692 7937 202
Size: 1013 245 1159
Fragments arranged by size:
2345 1159 1112 1013 723 635 553 435
360 314 245
NaeI GCC'GGC
Cuts at: 7075 7075
Size: 8894
NarI GG'CG_CC
Cuts at: 6325 6573 6325
Size: 248 8646
NcoI C'CATG_G
Cuts at: 107 488 1493 1580 3149 107
Size: 381 1005 87 1569 5852
Fragments arranged by size:
5852 1569 1005 381 87
NgoMIV G'CCGG_C
Cuts at: 7073 7073
Size: 8894
NspI r_CATG'y
Cuts at: 127 291 1098 2379 4200 5947 6447 7354 127
Size: 164 807 1281 1821 1747 500 907 1667
Fragments arranged by size:
1821 1747 1667 1281 907 807 500 164
PasI CC'CwG_GG
Cuts at: 2294 3349 3558 3857 2294
Size: 1055 209 299 7331
Fragments arranged by size:
7331 1055 299 209
PciI A'CATG_T
Cuts at: 287 7350 287
Size: 7063 1831
PflMI CCAn_nnn'nTGG
Cuts at: 1475 1850 2085 2900 3614 3801 4463 4683 5257
Size: 375 235 815 714 187 662 220 574
Cuts at: 5257 1475
Size: 5112
Fragments arranged by size:
5112 815 714 662 574 375 235 220
187
PfoI T'CCnGG_A
Cuts at: 785 1480 1664 3188 4767 785
Size: 695 184 1524 1579 4912
Fragments arranged by size:
4912 1579 1524 695 184
PmeI GTTT'AAAC
Cuts at: 5893 5893
Size: 8894
PmlI CAC'GTG
Cuts at: 1896 2244 1896
Size: 348 8546
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2333 2365 3676 3708 4749 4781 5698 5730 8033
Size: 32 1311 32 1041 32 917 32 2303
Cuts at: 8033 8065 2333
Size: 32 3162
Fragments arranged by size:
3162 2303 1311 1041 917 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 597 1602 1953 2045 3171 3360 3513 3816 4590
Size: 1005 351 92 1126 189 153 303 774
Cuts at: 4590 597
Size: 4901
Fragments arranged by size:
4901 1126 1005 774 351 303 189 153
92
PsiI TTA'TAA
Cuts at: 368 368
Size: 8894
PstI C_TGCA'G
Cuts at: 1244 1622 3551 3644 4427 5067 5941 6626 1244
Size: 378 1929 93 783 640 874 685 3512
Fragments arranged by size:
3512 1929 874 783 685 640 378 93
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8894
PvuII CAG'CTG
Cuts at: 2982 6319 6679 2982
Size: 3337 360 5197
Fragments arranged by size:
5197 3337 360
RsrII CG'GwC_CG
Cuts at: 7089 7089
Size: 8894
SacI G_AGCT'C
Cuts at: 794 5908 794
Size: 5114 3780
SalI G'TCGA_C
Cuts at: 5931 5931
Size: 8894
SanDI GG'GwC_CC
Cuts at: 3513 3513
Size: 8894
SapI GCTCTTCn'nnn_
Cuts at: 1003 4678 6917 7127 1003
Size: 3675 2239 210 2770
Fragments arranged by size:
3675 2770 2239 210
SbfI CC_TGCA'GG
Cuts at: 1244 1622 5941 1244
Size: 378 4319 4197
Fragments arranged by size:
4319 4197 378
ScaI AGT'ACT
Cuts at: 5821 8411 5821
Size: 2590 6304
SexAI A'CCwGG_T
Cuts at: 4492 4492
Size: 8894
SfcI C'TryA_G
Cuts at: 33 1240 1558 1618 3547 3640 4014 4423 5063
Size: 1207 318 60 1929 93 374 409 640
Cuts at: 5063 5550 5639 5937 6622 7615 7806 33
Size: 487 89 298 685 993 191 1121
Fragments arranged by size:
1929 1207 1121 993 685 640 487 409
374 318 298 191 93 89 60
SfoI GGC'GCC
Cuts at: 6326 6574 6326
Size: 248 8646
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8894
SmaI CCC'GGG
Cuts at: 5916 5916
Size: 8894
SmlI C'TyrA_G
Cuts at: 457 466 1831 2415 2455 6049 6964 7456 7718
Size: 9 1365 584 40 3594 915 492 262
Cuts at: 7718 457
Size: 1633
Fragments arranged by size:
3594 1633 1365 915 584 492 262 40
9
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8894
SphI G_CATG'C
Cuts at: 2379 4200 5947 6447 2379
Size: 1821 1747 500 4826
Fragments arranged by size:
4826 1821 1747 500
StuI AGG'CCT
Cuts at: 1947 3025 4140 4785 4840 5069 1947
Size: 1078 1115 645 55 229 5772
Fragments arranged by size:
5772 1115 1078 645 229 55
StyI C'CwwG_G
Cuts at: 107 488 1493 1580 2654 2741 3149 5569 6028
Size: 381 1005 87 1074 87 408 2420 459
Cuts at: 6028 6382 107
Size: 354 2619
Fragments arranged by size:
2619 2420 1074 1005 459 408 381 354
87 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 376 427 822 1707 1728 3554 3777 6477 6610
Size: 51 395 885 21 1826 223 2700 133
Cuts at: 6610 7069 7278 376
Size: 459 209 1992
Fragments arranged by size:
2700 1992 1826 885 459 395 223 209
133 51 21
TatI w'GTAC_w
Cuts at: 1457 3481 4850 5819 8409 1457
Size: 2024 1369 969 2590 1942
Fragments arranged by size:
2590 2024 1942 1369 969
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 189 221 380 402 412 434 3497 3529 3618
Size: 32 159 22 10 22 3063 32 89
Cuts at: 3618 3650 3808 3840 4589 4621 4679 4711 8411
Size: 32 158 32 749 32 58 32 3700
Cuts at: 8411 8443 189
Size: 32 640
Fragments arranged by size:
3700 3063 749 640 159 158 89 58
32 32 32 32 32 32 32 22
22 10
Tth111I GACn'n_nGTC
Cuts at: 832 6691 832
Size: 5859 3035
XbaI T'CTAG_A
Cuts at: 5925 5925
Size: 8894
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4374 6389 4374
Size: 2015 6879
XmaI C'CCGG_G
Cuts at: 5914 5914
Size: 8894
XmnI GAAnn'nnTTC
Cuts at: 934 2644 3691 934
Size: 1710 1047 6137
Fragments arranged by size:
6137 1710 1047
ZraI GAC'GTC
Cuts at: 1425 3714 1425
Size: 2289 6605
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AleI AlfI ApaI AscI AvrII BaeI BfrBI BglI
BmtI BssHII BstZ17I DraIII FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PshAI PspOMI
PspXI PsrI SacII SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI