Restriction map for
pF1KE2533
(Circular) (Six-base) MAPSORT of: pF1KE2533.seq Check: 9055 from: 1 to: 4691
pF1KE2533 4691 bp
With 182 enzymes: SgfI *
July 9, 2014 09:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 912 912
Size: 4691
AccI GT'mk_AC
Cuts at: 527 1729 527
Size: 1202 3489
Acc65I G'GTAC_C
Cuts at: 1707 1707
Size: 4691
AclI AA'CG_TT
Cuts at: 4531 4531
Size: 4691
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 824 1613 2516 2948 3695 3963 824
Size: 789 903 432 747 268 1552
Fragments arranged by size:
1552 903 789 747 432 268
AflIII A'CryG_T
Cuts at: 2043 3147 4124 2043
Size: 1104 977 2610
Fragments arranged by size:
2610 1104 977
AgeI A'CCGG_T
Cuts at: 3079 3079
Size: 4691
AlwNI CAG_nnn'CTG
Cuts at: 1024 1996 3563 1024
Size: 972 1567 2152
Fragments arranged by size:
2152 1567 972
ApaI G_GGCC'C
Cuts at: 386 386
Size: 4691
ApaLI G'TGCA_C
Cuts at: 3461 3461
Size: 4691
ApoI r'AATT_y
Cuts at: 1695 4044 4300 1695
Size: 2349 256 2086
Fragments arranged by size:
2349 2086 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4691
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4691
AvaI C'yCGr_G
Cuts at: 1711 1711
Size: 4691
BamHI G'GATC_C
Cuts at: 991 1716 991
Size: 725 3966
BanI G'GyrC_C
Cuts at: 113 1707 2121 2369 2404 113
Size: 1594 414 248 35 2400
Fragments arranged by size:
2400 1594 414 248 35
BanII G_rGCy'C
Cuts at: 386 959 1705 2735 386
Size: 573 746 1030 2342
Fragments arranged by size:
2342 1030 746 573
BbeI G_GCGC'C
Cuts at: 2125 2373 2125
Size: 248 4443
BbsI GAAGACnn'nnnn_
Cuts at: 1293 1480 1293
Size: 187 4504
BbvCI CC'TCA_GC
Cuts at: 871 1249 871
Size: 378 4313
BciVI GTATCCnnnnn_n'
Cuts at: 2589 3350 4691 2589
Size: 761 1341 2589
Fragments arranged by size:
2589 1341 761
BclI T'GATC_A
Cuts at: 2211 2211
Size: 4691
BfrBI ATG'CAT
Cuts at: 421 421
Size: 4691
BglII A'GATC_T
Cuts at: 489 2206 4243 489
Size: 1717 2037 937
Fragments arranged by size:
2037 1717 937
BlpI GC'TnA_GC
Cuts at: 1803 1803
Size: 4691
Bme1580I G_kGCm'C
Cuts at: 116 386 2316 2409 3465 116
Size: 270 1930 93 1056 1342
Fragments arranged by size:
1930 1342 1056 270 93
BmrI ACTGGGnnnn_n'
Cuts at: 760 784 1903 1984 2319 4463 760
Size: 24 1119 81 335 2144 988
Fragments arranged by size:
2144 1119 988 335 81 24
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3058 3058
Size: 4691
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 540 540
Size: 4691
Bpu10I CC'TnA_GC
Cuts at: 871 1249 871
Size: 378 4313
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1867 2746 3238 3536 1867
Size: 879 492 298 3022
Fragments arranged by size:
3022 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4395 4395
Size: 4691
BsaAI yAC'GTr
Cuts at: 2044 2044
Size: 4691
BsaBI GATnn'nnATC
Cuts at: 6 2205 4242 6
Size: 2199 2037 455
Fragments arranged by size:
2199 2037 455
BsaHI Gr'CG_yC
Cuts at: 2122 2370 3072 4568 2122
Size: 248 702 1496 2245
Fragments arranged by size:
2245 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1880 2401 3079 3353 3500 3911 1880
Size: 521 678 274 147 411 2660
Fragments arranged by size:
2660 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 209 284 1279 1607 2116 2874 3451 3947 209
Size: 75 995 328 509 758 577 496 953
Fragments arranged by size:
995 953 758 577 509 496 328 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 890 890
Size: 4691
BsiEI CG_ry'CG
Cuts at: 104 399 2233 2279 3487 104
Size: 295 1834 46 1208 1308
Fragments arranged by size:
1834 1308 1208 295 46
BsiHKAI G_wGCw'C
Cuts at: 959 1514 1705 2483 2673 3465 959
Size: 555 191 778 190 792 2185
Fragments arranged by size:
2185 792 778 555 191 190
Bsp1286I G_dGCh'C
Cuts at: 116 386 959 1514 1705 2316 2409 2483 2673
Size: 270 573 555 191 611 93 74 190
Cuts at: 2673 2735 3465 116
Size: 62 730 1342
Fragments arranged by size:
1342 730 611 573 555 270 191 190
93 74 62
BspEI T'CCGG_A
Cuts at: 3911 3911
Size: 4691
BspHI T'CATG_A
Cuts at: 3867 3867
Size: 4691
BspMI ACCTGCnnnn'nnnn_
Cuts at: 912 1009 1156 1674 1741 1955 2257 2638 912
Size: 97 147 518 67 214 302 381 2965
Fragments arranged by size:
2965 518 381 302 214 147 97 67
BsrBI CCG'CTC
Cuts at: 50 2983 3037 4521 4686 50
Size: 2933 54 1484 165 55
Fragments arranged by size:
2933 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1394 2603 1394
Size: 1209 3482
BsrFI r'CCGG_y
Cuts at: 1477 2689 2870 3079 1477
Size: 1212 181 209 3089
Fragments arranged by size:
3089 1212 209 181
BssSI C'ACGA_G
Cuts at: 2962 3320 2962
Size: 358 4333
BstAPI GCAn_nnn'nTGC
Cuts at: 2068 2068
Size: 4691
BstBI TT'CG_AA
Cuts at: 3052 3052
Size: 4691
BstXI CCAn_nnnn'nTGG
Cuts at: 576 576
Size: 4691
BstYI r'GATC_y
Cuts at: 1 489 991 1716 2183 2206 2541 2787 3799
Size: 488 502 725 467 23 335 246 1012
Cuts at: 3799 3885 3897 4243 1
Size: 86 12 346 449
Fragments arranged by size:
1012 725 502 488 467 449 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 528 528
Size: 4691
BtgI C'CryG_G
Cuts at: 107 107
Size: 4691
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2641 2822 2985 2641
Size: 181 163 4347
Fragments arranged by size:
4347 181 163
BtsI GCAGTG_nn'
Cuts at: 203 789 1934 203
Size: 586 1145 2960
Fragments arranged by size:
2960 1145 586
DraI TTT'AAA
Cuts at: 1690 1690
Size: 4691
DraIII CAC_nnn'GTG
Cuts at: 138 138
Size: 4691
DrdI GACnn_nn'nnGTC
Cuts at: 2397 3255 2397
Size: 858 3833
EaeI y'GGCC_r
Cuts at: 2276 2450 2841 2868 4213 2276
Size: 174 391 27 1345 2754
Fragments arranged by size:
2754 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2276 2276
Size: 4691
EarI CTCTTCn'nnn_
Cuts at: 2714 2924 2714
Size: 210 4481
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2899 3209 3355 4501 2899
Size: 310 146 1146 3089
Fragments arranged by size:
3089 1146 310 146
EcoICRI GAG'CTC
Cuts at: 957 1703 957
Size: 746 3945
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 540 824 1613 2516 2948 3695 3963 540
Size: 284 789 903 432 747 268 1268
Fragments arranged by size:
1268 903 789 747 432 284 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1215 1215
Size: 4691
EcoO109I rG'GnC_Cy
Cuts at: 382 701 739 1830 382
Size: 319 38 1091 3243
Fragments arranged by size:
3243 1091 319 38
EcoRI G'AATT_C
Cuts at: 1695 1695
Size: 4691
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2710 2710
Size: 4691
FspI TGC'GCA
Cuts at: 2472 2472
Size: 4691
HaeII r_GCGC'y
Cuts at: 2125 2373 3395 4374 2125
Size: 248 1022 979 2442
Fragments arranged by size:
2442 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 636 668 693 725 1117 1149 2533 2563 2565
Size: 32 25 32 392 32 1384 30 2
Cuts at: 2565 2595 636
Size: 30 2732
Fragments arranged by size:
2732 1384 392 32 32 32 30 30
25 2
HincII GTy'rAC
Cuts at: 1730 1730
Size: 4691
KasI G'GCGC_C
Cuts at: 2121 2369 2121
Size: 248 4443
KpnI G_GTAC'C
Cuts at: 1711 1711
Size: 4691
MluI A'CGCG_T
Cuts at: 4124 4124
Size: 4691
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 593 1553 3362 3546 593
Size: 960 1809 184 1738
Fragments arranged by size:
1809 1738 960 184
MscI TGG'CCA
Cuts at: 2452 2452
Size: 4691
MslI CAynn'nnrTG
Cuts at: 976 1550 2680 2807 976
Size: 574 1130 127 2860
Fragments arranged by size:
2860 1130 574 127
MspA1I CmG'CkG
Cuts at: 305 395 684 1802 2116 2476 3489 3734 305
Size: 90 289 1118 314 360 1013 245 1262
Fragments arranged by size:
1262 1118 1013 360 314 289 245 90
NaeI GCC'GGC
Cuts at: 2872 2872
Size: 4691
NarI GG'CG_CC
Cuts at: 2122 2370 2122
Size: 248 4443
NcoI C'CATG_G
Cuts at: 107 107
Size: 4691
NgoMIV G'CCGG_C
Cuts at: 2870 2870
Size: 4691
NsiI A_TGCA'T
Cuts at: 423 423
Size: 4691
NspI r_CATG'y
Cuts at: 1457 1744 2244 3151 1457
Size: 287 500 907 2997
Fragments arranged by size:
2997 907 500 287
PasI CC'CwG_GG
Cuts at: 379 1233 379
Size: 854 3837
PciI A'CATG_T
Cuts at: 3147 3147
Size: 4691
PflMI CCAn_nnn'nTGG
Cuts at: 1181 1181
Size: 4691
PmeI GTTT'AAAC
Cuts at: 1690 1690
Size: 4691
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3830 3862 3830
Size: 32 4659
PpuMI rG'GwC_Cy
Cuts at: 701 739 701
Size: 38 4653
PshAI GACnn'nnGTC
Cuts at: 1042 1114 1042
Size: 72 4619
PspOMI G'GGCC_C
Cuts at: 382 382
Size: 4691
PstI C_TGCA'G
Cuts at: 683 1738 2423 683
Size: 1055 685 2951
Fragments arranged by size:
2951 1055 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4691
PvuII CAG'CTG
Cuts at: 305 684 2116 2476 305
Size: 379 1432 360 2520
Fragments arranged by size:
2520 1432 379 360
RsrII CG'GwC_CG
Cuts at: 2886 2886
Size: 4691
SacI G_AGCT'C
Cuts at: 959 1705 959
Size: 746 3945
SalI G'TCGA_C
Cuts at: 1728 1728
Size: 4691
SapI GCTCTTCn'nnn_
Cuts at: 2714 2924 2714
Size: 210 4481
SbfI CC_TGCA'GG
Cuts at: 1738 1738
Size: 4691
ScaI AGT'ACT
Cuts at: 913 4208 913
Size: 3295 1396
SfcI C'TryA_G
Cuts at: 33 300 679 1734 2419 3412 3603 33
Size: 267 379 1055 685 993 191 1121
Fragments arranged by size:
1121 1055 993 685 379 267 191
SfoI GGC'GCC
Cuts at: 2123 2371 2123
Size: 248 4443
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4691
SmaI CCC'GGG
Cuts at: 1713 1713
Size: 4691
SmlI C'TyrA_G
Cuts at: 1846 2761 3253 3515 1846
Size: 915 492 262 3022
Fragments arranged by size:
3022 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4691
SphI G_CATG'C
Cuts at: 1744 2244 1744
Size: 500 4191
StyI C'CwwG_G
Cuts at: 107 1825 2179 107
Size: 1718 354 2619
Fragments arranged by size:
2619 1718 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 460 2274 2407 2866 3075 460
Size: 1814 133 459 209 2076
Fragments arranged by size:
2076 1814 459 209 133
TatI w'GTAC_w
Cuts at: 911 4206 911
Size: 3295 1396
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4208 4240 4208
Size: 32 4659
Tth111I GACn'n_nGTC
Cuts at: 790 2488 790
Size: 1698 2993
XbaI T'CTAG_A
Cuts at: 1722 1722
Size: 4691
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 292 2186 292
Size: 1894 2797
XmaI C'CCGG_G
Cuts at: 1711 1711
Size: 4691
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BcgI BglI BmgBI BmtI BsaXI BseRI
BsiWI BsmI BsmBI BsrGI BssHII BstEII Bsu36I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI PacI PfoI PmlI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI