Restriction map for
pF1KE2536
(Circular) (Six-base) MAPSORT of: pF1KE2536.seq Check: 8682 from: 1 to: 3482
pF1KE2536 3482 bp
With 182 enzymes: SgfI *
July 9, 2014 09:50 ..
AccI GT'mk_AC
Cuts at: 520 520
Size: 3482
Acc65I G'GTAC_C
Cuts at: 498 498
Size: 3482
AclI AA'CG_TT
Cuts at: 3322 3322
Size: 3482
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1307 1739 2486 2754 1307
Size: 432 747 268 2035
Fragments arranged by size:
2035 747 432 268
AflIII A'CryG_T
Cuts at: 834 1938 2915 834
Size: 1104 977 1401
Fragments arranged by size:
1401 1104 977
AgeI A'CCGG_T
Cuts at: 1870 1870
Size: 3482
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 241 241
Size: 3482
AlwNI CAG_nnn'CTG
Cuts at: 787 2354 787
Size: 1567 1915
ApaI G_GGCC'C
Cuts at: 435 435
Size: 3482
ApaLI G'TGCA_C
Cuts at: 2252 2252
Size: 3482
ApoI r'AATT_y
Cuts at: 486 2835 3091 486
Size: 2349 256 877
Fragments arranged by size:
2349 877 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3482
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3482
AvaI C'yCGr_G
Cuts at: 118 428 502 118
Size: 310 74 3098
Fragments arranged by size:
3098 310 74
BamHI G'GATC_C
Cuts at: 507 507
Size: 3482
BanI G'GyrC_C
Cuts at: 379 498 912 1160 1195 379
Size: 119 414 248 35 2666
Fragments arranged by size:
2666 414 248 119 35
BanII G_rGCy'C
Cuts at: 435 496 1526 435
Size: 61 1030 2391
Fragments arranged by size:
2391 1030 61
BbeI G_GCGC'C
Cuts at: 916 1164 916
Size: 248 3234
BbsI GAAGACnn'nnnn_
Cuts at: 288 288
Size: 3482
BciVI GTATCCnnnnn_n'
Cuts at: 1380 2141 3482 1380
Size: 761 1341 1380
Fragments arranged by size:
1380 1341 761
BclI T'GATC_A
Cuts at: 1002 1002
Size: 3482
BglII A'GATC_T
Cuts at: 997 3034 997
Size: 2037 1445
BlpI GC'TnA_GC
Cuts at: 594 594
Size: 3482
Bme1580I G_kGCm'C
Cuts at: 382 435 1107 1200 2256 382
Size: 53 672 93 1056 1608
Fragments arranged by size:
1608 1056 672 93 53
BmrI ACTGGGnnnn_n'
Cuts at: 386 694 775 1110 3254 386
Size: 308 81 335 2144 614
Fragments arranged by size:
2144 614 335 308 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 426 1849 426
Size: 1423 2059
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 228 228
Size: 3482
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 658 1537 2029 2327 658
Size: 879 492 298 1813
Fragments arranged by size:
1813 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3186 3186
Size: 3482
BsaAI yAC'GTr
Cuts at: 835 835
Size: 3482
BsaBI GATnn'nnATC
Cuts at: 6 996 3033 6
Size: 990 2037 455
Fragments arranged by size:
2037 990 455
BsaHI Gr'CG_yC
Cuts at: 913 1161 1863 3359 913
Size: 248 702 1496 1036
Fragments arranged by size:
1496 1036 702 248
BsaWI w'CCGG_w
Cuts at: 671 1192 1870 2144 2291 2702 671
Size: 521 678 274 147 411 1451
Fragments arranged by size:
1451 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 415 415
Size: 3482
BseYI C'CCAG_C
Cuts at: 123 141 167 907 1665 2242 2738 123
Size: 18 26 740 758 577 496 867
Fragments arranged by size:
867 758 740 577 496 26 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 478 478
Size: 3482
BsiEI CG_ry'CG
Cuts at: 104 1024 1070 2278 104
Size: 920 46 1208 1308
Fragments arranged by size:
1308 1208 920 46
BsiHKAI G_wGCw'C
Cuts at: 496 1274 1464 2256 496
Size: 778 190 792 1722
Fragments arranged by size:
1722 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 445 445
Size: 3482
Bsp1286I G_dGCh'C
Cuts at: 382 435 496 1107 1200 1274 1464 1526 2256
Size: 53 61 611 93 74 190 62 730
Cuts at: 2256 382
Size: 1608
Fragments arranged by size:
1608 730 611 190 93 74 62 61
53
BspEI T'CCGG_A
Cuts at: 2702 2702
Size: 3482
BspHI T'CATG_A
Cuts at: 2658 2658
Size: 3482
BspMI ACCTGCnnnn'nnnn_
Cuts at: 269 532 746 1048 1429 269
Size: 263 214 302 381 2322
Fragments arranged by size:
2322 381 302 263 214
BsrBI CCG'CTC
Cuts at: 50 1774 1828 3312 3477 50
Size: 1724 54 1484 165 55
Fragments arranged by size:
1724 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1394 1394
Size: 3482
BsrFI r'CCGG_y
Cuts at: 386 1480 1661 1870 386
Size: 1094 181 209 1998
Fragments arranged by size:
1998 1094 209 181
BssSI C'ACGA_G
Cuts at: 1753 2111 1753
Size: 358 3124
BstAPI GCAn_nnn'nTGC
Cuts at: 859 859
Size: 3482
BstBI TT'CG_AA
Cuts at: 1843 1843
Size: 3482
BstYI r'GATC_y
Cuts at: 1 507 974 997 1332 1578 2590 2676 2688
Size: 506 467 23 335 246 1012 86 12
Cuts at: 2688 3034 1
Size: 346 449
Fragments arranged by size:
1012 506 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 437 437
Size: 3482
BtgI C'CryG_G
Cuts at: 107 107
Size: 3482
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1432 1613 1776 1432
Size: 181 163 3138
Fragments arranged by size:
3138 181 163
BtsI GCAGTG_nn'
Cuts at: 278 725 278
Size: 447 3035
DraI TTT'AAA
Cuts at: 481 481
Size: 3482
DrdI GACnn_nn'nnGTC
Cuts at: 1188 2046 1188
Size: 858 2624
EaeI y'GGCC_r
Cuts at: 1067 1241 1632 1659 3004 1067
Size: 174 391 27 1345 1545
Fragments arranged by size:
1545 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1067 1067
Size: 3482
EarI CTCTTCn'nnn_
Cuts at: 1505 1715 1505
Size: 210 3272
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1690 2000 2146 3292 1690
Size: 310 146 1146 1880
Fragments arranged by size:
1880 1146 310 146
EcoICRI GAG'CTC
Cuts at: 494 494
Size: 3482
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 228 1307 1739 2486 2754 228
Size: 1079 432 747 268 956
Fragments arranged by size:
1079 956 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 432 621 432
Size: 189 3293
EcoRI G'AATT_C
Cuts at: 486 486
Size: 3482
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1501 1501
Size: 3482
FspI TGC'GCA
Cuts at: 1263 1263
Size: 3482
HaeII r_GCGC'y
Cuts at: 142 916 1164 2186 3165 142
Size: 774 248 1022 979 459
Fragments arranged by size:
1022 979 774 459 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1324 1354 1356 1386 1324
Size: 30 2 30 3420
Fragments arranged by size:
3420 30 30 2
HincII GTy'rAC
Cuts at: 521 521
Size: 3482
HindIII A'AGCT_T
Cuts at: 352 352
Size: 3482
KasI G'GCGC_C
Cuts at: 912 1160 912
Size: 248 3234
KpnI G_GTAC'C
Cuts at: 502 502
Size: 3482
MluI A'CGCG_T
Cuts at: 2915 2915
Size: 3482
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2153 2337 2153
Size: 184 3298
MscI TGG'CCA
Cuts at: 1243 1243
Size: 3482
MslI CAynn'nnrTG
Cuts at: 1471 1598 1471
Size: 127 3355
MspA1I CmG'CkG
Cuts at: 167 593 907 1267 2280 2525 167
Size: 426 314 360 1013 245 1124
Fragments arranged by size:
1124 1013 426 360 314 245
NaeI GCC'GGC
Cuts at: 1663 1663
Size: 3482
NarI GG'CG_CC
Cuts at: 913 1161 913
Size: 248 3234
NcoI C'CATG_G
Cuts at: 107 107
Size: 3482
NgoMIV G'CCGG_C
Cuts at: 1661 1661
Size: 3482
NspI r_CATG'y
Cuts at: 535 1035 1942 535
Size: 500 907 2075
Fragments arranged by size:
2075 907 500
PciI A'CATG_T
Cuts at: 1938 1938
Size: 3482
PmeI GTTT'AAAC
Cuts at: 481 481
Size: 3482
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 391 423 2621 2653 391
Size: 32 2198 32 1220
Fragments arranged by size:
2198 1220 32 32
PspOMI G'GGCC_C
Cuts at: 431 431
Size: 3482
PstI C_TGCA'G
Cuts at: 529 1214 529
Size: 685 2797
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3482
PvuII CAG'CTG
Cuts at: 907 1267 907
Size: 360 3122
RsrII CG'GwC_CG
Cuts at: 1677 1677
Size: 3482
SacI G_AGCT'C
Cuts at: 496 496
Size: 3482
SalI G'TCGA_C
Cuts at: 519 519
Size: 3482
SapI GCTCTTCn'nnn_
Cuts at: 1505 1715 1505
Size: 210 3272
SbfI CC_TGCA'GG
Cuts at: 529 529
Size: 3482
ScaI AGT'ACT
Cuts at: 2999 2999
Size: 3482
SfcI C'TryA_G
Cuts at: 33 525 1210 2203 2394 33
Size: 492 685 993 191 1121
Fragments arranged by size:
1121 993 685 492 191
SfoI GGC'GCC
Cuts at: 914 1162 914
Size: 248 3234
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3482
SmaI CCC'GGG
Cuts at: 120 430 504 120
Size: 310 74 3098
Fragments arranged by size:
3098 310 74
SmlI C'TyrA_G
Cuts at: 637 1552 2044 2306 637
Size: 915 492 262 1813
Fragments arranged by size:
1813 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3482
SphI G_CATG'C
Cuts at: 535 1035 535
Size: 500 2982
SrfI GCCC'GGGC
Cuts at: 430 430
Size: 3482
StyI C'CwwG_G
Cuts at: 107 446 616 970 107
Size: 339 170 354 2619
Fragments arranged by size:
2619 354 339 170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1065 1198 1657 1866 1065
Size: 133 459 209 2681
Fragments arranged by size:
2681 459 209 133
TatI w'GTAC_w
Cuts at: 2997 2997
Size: 3482
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2999 3031 2999
Size: 32 3450
Tth111I GACn'n_nGTC
Cuts at: 1279 1279
Size: 3482
XbaI T'CTAG_A
Cuts at: 513 513
Size: 3482
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 977 977
Size: 3482
XmaI C'CCGG_G
Cuts at: 118 428 502 118
Size: 310 74 3098
Fragments arranged by size:
3098 310 74
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SspI
StuI SwaI XhoI XmnI ZraI