Restriction map for
pF1KE2538
(Circular) (Six-base) MAPSORT of: pF1KE2538.seq Check: 623 from: 1 to: 10805
pF1KE2538 10805 bp
With 182 enzymes: SgfI *
February 10, 2017 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 702 6270 702
Size: 5568 5237
AccI GT'mk_AC
Cuts at: 437 656 3202 3792 4041 7843 437
Size: 219 2546 590 249 3802 3399
Fragments arranged by size:
3802 3399 2546 590 249 219
Acc65I G'GTAC_C
Cuts at: 7821 7821
Size: 10805
AclI AA'CG_TT
Cuts at: 5095 10645 5095
Size: 5550 5255
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 321 415 1070 1655 2534 2549 2740 4171
Size: 124 94 655 585 879 15 191 1431
Cuts at: 4171 4354 4452 4954 5063 5428 5479 6231 7075
Size: 183 98 502 109 365 51 752 844
Cuts at: 7075 7634 7690 8630 9062 9809 10077 197
Size: 559 56 940 432 747 268 925
Fragments arranged by size:
1431 940 925 879 844 752 747 655
585 559 502 432 365 268 191 183
124 109 98 94 56 51 15
AflIII A'CryG_T
Cuts at: 903 3072 3840 3994 6789 7009 8157 9261 10238
Size: 2169 768 154 2795 220 1148 1104 977
Cuts at: 10238 903
Size: 1470
Fragments arranged by size:
2795 2169 1470 1148 1104 977 768 220
154
AgeI A'CCGG_T
Cuts at: 9193 9193
Size: 10805
AhdI GACnn_n'nnGTC
Cuts at: 4200 4200
Size: 10805
AleI CACnn'nnGTG
Cuts at: 4997 4997
Size: 10805
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2181 2181
Size: 10805
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1706 1738 1706
Size: 32 10773
AlwNI CAG_nnn'CTG
Cuts at: 1075 2149 2695 5710 5974 6537 6802 7563 8110
Size: 1074 546 3015 264 563 265 761 547
Cuts at: 8110 9677 1075
Size: 1567 2203
Fragments arranged by size:
3015 2203 1567 1074 761 563 547 546
265 264
ApaI G_GGCC'C
Cuts at: 4448 4448
Size: 10805
ApaLI G'TGCA_C
Cuts at: 9575 9575
Size: 10805
ApoI r'AATT_y
Cuts at: 530 980 1126 1810 1818 2023 2625 2644 3006
Size: 450 146 684 8 205 602 19 362
Cuts at: 3006 3123 3330 3454 3533 3660 4171 6179 7809
Size: 117 207 124 79 127 511 2008 1630
Cuts at: 7809 10158 10414 530
Size: 2349 256 921
Fragments arranged by size:
2349 2008 1630 921 684 602 511 450
362 256 207 205 146 127 124 117
79 19 8
AseI AT'TA_AT
Cuts at: 20 1916 6029 20
Size: 1896 4113 4796
Fragments arranged by size:
4796 4113 1896
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10805
AvaI C'yCGr_G
Cuts at: 4880 7301 7825 4880
Size: 2421 524 7860
Fragments arranged by size:
7860 2421 524
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2438 2471 3946 3979 2438
Size: 33 1475 33 9264
Fragments arranged by size:
9264 1475 33 33
BamHI G'GATC_C
Cuts at: 4026 4144 6639 7359 7830 4026
Size: 118 2495 720 471 7001
Fragments arranged by size:
7001 2495 720 471 118
BanI G'GyrC_C
Cuts at: 280 6783 7821 8235 8483 8518 280
Size: 6503 1038 414 248 35 2567
Fragments arranged by size:
6503 2567 1038 414 248 35
BanII G_rGCy'C
Cuts at: 1171 1420 3872 4448 4638 5993 7819 8849 1171
Size: 249 2452 576 190 1355 1826 1030 3127
Fragments arranged by size:
3127 2452 1826 1355 1030 576 249 190
BbeI G_GCGC'C
Cuts at: 8239 8487 8239
Size: 248 10557
BbsI GAAGACnn'nnnn_
Cuts at: 1513 2755 3039 3143 4669 6277 7081 7300 7622
Size: 1242 284 104 1526 1608 804 219 322
Cuts at: 7622 1513
Size: 4696
Fragments arranged by size:
4696 1608 1526 1242 804 322 284 219
104
BbvCI CC'TCA_GC
Cuts at: 7190 7190
Size: 10805
BciVI GTATCCnnnnn_n'
Cuts at: 8703 9464 10805 8703
Size: 761 1341 8703
Fragments arranged by size:
8703 1341 761
BclI T'GATC_A
Cuts at: 2088 5835 6717 7337 8325 2088
Size: 3747 882 620 988 4568
Fragments arranged by size:
4568 3747 988 882 620
BfrBI ATG'CAT
Cuts at: 2249 3160 2249
Size: 911 9894
BglII A'GATC_T
Cuts at: 814 1318 8320 10357 814
Size: 504 7002 2037 1262
Fragments arranged by size:
7002 2037 1262 504
BlpI GC'TnA_GC
Cuts at: 1170 5314 5698 6725 7917 1170
Size: 4144 384 1027 1192 4058
Fragments arranged by size:
4144 4058 1192 1027 384
Bme1580I G_kGCm'C
Cuts at: 4424 4448 8430 8523 9579 4424
Size: 24 3982 93 1056 5650
Fragments arranged by size:
5650 3982 1056 93 24
BmrI ACTGGGnnnn_n'
Cuts at: 1726 3805 4032 4064 6688 6816 6892 7539 8017
Size: 2079 227 32 2624 128 76 647 478
Cuts at: 8017 8098 8433 10577 1726
Size: 81 335 2144 1954
Fragments arranged by size:
2624 2144 2079 1954 647 478 335 227
128 81 76 32
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1337 1359 4873 5310 9172 1337
Size: 22 3514 437 3862 2970
Fragments arranged by size:
3862 3514 2970 437 22
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 401 1934 2807 3289 6846 7479 7717 401
Size: 1533 873 482 3557 633 238 3489
Fragments arranged by size:
3557 3489 1533 873 633 482 238
Bpu10I CC'TnA_GC
Cuts at: 7190 7190
Size: 10805
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1500 2978 3881 4061 4622 4818 6965 7420 7981
Size: 1478 903 180 561 196 2147 455 561
Cuts at: 7981 8860 9352 9650 1500
Size: 879 492 298 2655
Fragments arranged by size:
2655 2147 1478 903 879 561 561 492
455 298 196 180
BsaI GGTCTCn'nnnn_
Cuts at: 1544 1594 1952 5296 5594 6290 10509 1544
Size: 50 358 3344 298 696 4219 1840
Fragments arranged by size:
4219 3344 1840 696 358 298 50
BsaAI yAC'GTr
Cuts at: 906 3917 3997 8158 906
Size: 3011 80 4161 3553
Fragments arranged by size:
4161 3553 3011 80
BsaBI GATnn'nnATC
Cuts at: 6 1290 2717 5834 8319 10356 6
Size: 1284 1427 3117 2485 2037 455
Fragments arranged by size:
3117 2485 2037 1427 1284 455
BsaHI Gr'CG_yC
Cuts at: 8236 8484 9186 10682 8236
Size: 248 702 1496 8359
Fragments arranged by size:
8359 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3528 7574 7994 8515 9193 9467 9614 10025 3528
Size: 4046 420 521 678 274 147 411 4308
Fragments arranged by size:
4308 4046 678 521 420 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2724 2754 3059 3089 5706 5736 5876 5906 6052
Size: 30 305 30 2617 30 140 30 146
Cuts at: 6052 6082 6844 6874 7127 7157 7657 7687 2724
Size: 30 762 30 253 30 500 30 5842
Fragments arranged by size:
5842 2617 762 500 305 253 146 140
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 3074 4135 5192 6073 7244 3074
Size: 1061 1057 881 1171 6635
Fragments arranged by size:
6635 1171 1061 1057 881
BseYI C'CCAG_C
Cuts at: 1942 4790 6643 6682 7079 8230 8988 9565 10061
Size: 2848 1853 39 397 1151 758 577 496
Cuts at: 10061 1942
Size: 2686
Fragments arranged by size:
2848 2686 1853 1151 758 577 496 397
39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 362 6956 232
Size: 130 6594 4081
Fragments arranged by size:
6594 4081 130
BsiEI CG_ry'CG
Cuts at: 104 5475 8347 8393 9601 104
Size: 5371 2872 46 1208 1308
Fragments arranged by size:
5371 2872 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 167 1171 1420 1473 3872 4638 5315 5661 5704
Size: 1004 249 53 2399 766 677 346 43
Cuts at: 5704 6671 7819 8597 8787 9579 167
Size: 967 1148 778 190 792 1393
Fragments arranged by size:
2399 1393 1148 1004 967 792 778 766
677 346 249 190 53 43
BsmI GAATG_Cn'
Cuts at: 4601 5917 4601
Size: 1316 9489
BsmBI CGTCTCn'nnnn_
Cuts at: 3307 3307
Size: 10805
Bsp1286I G_dGCh'C
Cuts at: 167 1171 1420 1473 3872 4424 4448 4638 5315
Size: 1004 249 53 2399 552 24 190 677
Cuts at: 5315 5661 5704 5993 6671 7819 8430 8523 8597
Size: 346 43 289 678 1148 611 93 74
Cuts at: 8597 8787 8849 9579 167
Size: 190 62 730 1393
Fragments arranged by size:
2399 1393 1148 1004 730 678 677 611
552 346 289 249 190 190 93 74
62 53 43 24
BspEI T'CCGG_A
Cuts at: 3528 10025 3528
Size: 6497 4308
BspHI T'CATG_A
Cuts at: 3210 4682 4985 9981 3210
Size: 1472 303 4996 4034
Fragments arranged by size:
4996 4034 1472 303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 702 2320 6270 7855 8069 8371 8752 702
Size: 1618 3950 1585 214 302 381 2755
Fragments arranged by size:
3950 2755 1618 1585 381 302 214
BsrBI CCG'CTC
Cuts at: 50 6375 9097 9151 10635 10800 50
Size: 6325 2722 54 1484 165 55
Fragments arranged by size:
6325 2722 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 393 4020 7710 8717 393
Size: 3627 3690 1007 2481
Fragments arranged by size:
3690 3627 2481 1007
BsrFI r'CCGG_y
Cuts at: 6885 8803 8984 9193 6885
Size: 1918 181 209 8497
Fragments arranged by size:
8497 1918 209 181
BsrGI T'GTAC_A
Cuts at: 1831 4609 6951 7065 1831
Size: 2778 2342 114 5571
Fragments arranged by size:
5571 2778 2342 114
BssSI C'ACGA_G
Cuts at: 1472 9076 9434 1472
Size: 7604 358 2843
Fragments arranged by size:
7604 2843 358
BstAPI GCAn_nnn'nTGC
Cuts at: 6528 8182 6528
Size: 1654 9151
BstBI TT'CG_AA
Cuts at: 4822 9166 4822
Size: 4344 6461
BstEII G'GTnAC_C
Cuts at: 3652 5167 6604 3652
Size: 1515 1437 7853
Fragments arranged by size:
7853 1515 1437
BstXI CCAn_nnnn'nTGG
Cuts at: 4484 5070 5119 6144 6841 4484
Size: 586 49 1025 697 8448
Fragments arranged by size:
8448 1025 697 586 49
BstYI r'GATC_y
Cuts at: 1 814 1191 1318 2131 4026 4144 5447 5829
Size: 813 377 127 813 1895 118 1303 382
Cuts at: 5829 6639 7236 7305 7359 7830 8297 8320 8655
Size: 810 597 69 54 471 467 23 335
Cuts at: 8655 8901 9913 9999 10011 10357 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1895 1303 1012 813 813 810 597 471
467 449 382 377 346 335 246 127
118 86 69 54 23 12
Bsu36I CC'TnA_GG
Cuts at: 1555 7688 1555
Size: 6133 4672
BtgI C'CryG_G
Cuts at: 3131 3344 3131
Size: 213 10592
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4497 5636 8755 8936 9099 4497
Size: 1139 3119 181 163 6203
Fragments arranged by size:
6203 3119 1139 181 163
BtsI GCAGTG_nn'
Cuts at: 374 571 638 2843 4606 6553 7166 8048 374
Size: 197 67 2205 1763 1947 613 882 3131
Fragments arranged by size:
3131 2205 1947 1763 882 613 197 67
ClaI AT'CG_AT
Cuts at: 1965 1965
Size: 10805
DraI TTT'AAA
Cuts at: 1124 1161 1181 1815 1988 2870 4875 7623 7804
Size: 37 20 634 173 882 2005 2748 181
Cuts at: 7804 1124
Size: 4125
Fragments arranged by size:
4125 2748 2005 882 634 181 173 37
20
DrdI GACnn_nn'nnGTC
Cuts at: 7010 8511 9369 7010
Size: 1501 858 8446
Fragments arranged by size:
8446 1501 858
EaeI y'GGCC_r
Cuts at: 1450 5472 6131 8390 8564 8955 8982 10327 1450
Size: 4022 659 2259 174 391 27 1345 1928
Fragments arranged by size:
4022 2259 1928 1345 659 391 174 27
EagI C'GGCC_G
Cuts at: 5472 8390 5472
Size: 2918 7887
EarI CTCTTCn'nnn_
Cuts at: 434 1490 1545 1620 2759 3021 3234 3701 3860
Size: 1056 55 75 1139 262 213 467 159
Cuts at: 3860 4112 5495 5858 6050 6366 8828 9038 434
Size: 252 1383 363 192 316 2462 210 2201
Fragments arranged by size:
2462 2201 1383 1139 1056 467 363 316
262 252 213 210 192 159 75 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 665 1326 9013 9323 9469 10615 665
Size: 661 7687 310 146 1146 855
Fragments arranged by size:
7687 1146 855 661 310 146
EcoICRI GAG'CTC
Cuts at: 1169 1418 3870 4636 7817 1169
Size: 249 2452 766 3181 4157
Fragments arranged by size:
4157 3181 2452 766 249
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 321 401 415 1070 1655 1934 2534 2549
Size: 124 80 14 655 585 279 600 15
Cuts at: 2549 2740 2807 3289 4171 4354 4452 4954 5063
Size: 191 67 482 882 183 98 502 109
Cuts at: 5063 5428 5479 6231 6846 7075 7479 7634 7690
Size: 365 51 752 615 229 404 155 56
Cuts at: 7690 7717 8630 9062 9809 10077 197
Size: 27 913 432 747 268 925
Fragments arranged by size:
925 913 882 752 747 655 615 600
585 502 482 432 404 365 279 268
229 191 183 155 124 109 98 80
67 56 51 27 15 14
EcoNI CCTnn'n_nnAGG
Cuts at: 1365 2921 6166 1365
Size: 1556 3245 6004
Fragments arranged by size:
6004 3245 1556
EcoO109I rG'GnC_Cy
Cuts at: 4312 4445 4473 7692 7944 4312
Size: 133 28 3219 252 7173
Fragments arranged by size:
7173 3219 252 133 28
EcoRI G'AATT_C
Cuts at: 3006 3330 6179 7809 3006
Size: 324 2849 1630 6002
Fragments arranged by size:
6002 2849 1630 324
EcoRV GAT'ATC
Cuts at: 4299 4299
Size: 10805
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4684 5848 8824 4684
Size: 1164 2976 6665
Fragments arranged by size:
6665 2976 1164
FspI TGC'GCA
Cuts at: 8586 8586
Size: 10805
HaeII r_GCGC'y
Cuts at: 8239 8487 9509 10488 8239
Size: 248 1022 979 8556
Fragments arranged by size:
8556 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 463 495 868 900 925 957 2056 2088 2803
Size: 32 373 32 25 32 1099 32 715
Cuts at: 2803 2835 3026 3058 3622 3654 4018 4050 4186
Size: 32 191 32 564 32 364 32 136
Cuts at: 4186 4218 5180 5212 5635 5667 6052 6084 7212
Size: 32 962 32 423 32 385 32 1128
Cuts at: 7212 7244 8647 8677 8679 8709 463
Size: 32 1403 30 2 30 2559
Fragments arranged by size:
2559 1403 1128 1099 962 715 564 423
385 373 364 191 136 32 32 32
32 32 32 32 32 32 32 32
32 32 30 30 25 2
HincII GTy'rAC
Cuts at: 5093 7844 5093
Size: 2751 8054
HindIII A'AGCT_T
Cuts at: 5491 5491
Size: 10805
HpaI GTT'AAC
Cuts at: 5093 5093
Size: 10805
KasI G'GCGC_C
Cuts at: 8235 8483 8235
Size: 248 10557
KpnI G_GTAC'C
Cuts at: 7825 7825
Size: 10805
MfeI C'AATT_G
Cuts at: 5157 7061 5157
Size: 1904 8901
MluI A'CGCG_T
Cuts at: 10238 10238
Size: 10805
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1749 5531 6133 7039 7456 9476 9660 1749
Size: 3782 602 906 417 2020 184 2894
Fragments arranged by size:
3782 2894 2020 906 602 417 184
MscI TGG'CCA
Cuts at: 6133 8566 6133
Size: 2433 8372
MslI CAynn'nnrTG
Cuts at: 1388 2392 2708 3165 3443 3629 3845 4482 4757
Size: 1004 316 457 278 186 216 637 275
Cuts at: 4757 4997 8794 8921 1388
Size: 240 3797 127 3272
Fragments arranged by size:
3797 3272 1004 637 457 316 278 275
240 216 186 127
MspA1I CmG'CkG
Cuts at: 117 421 4997 5904 7916 8230 8590 9603 9848
Size: 304 4576 907 2012 314 360 1013 245
Cuts at: 9848 117
Size: 1074
Fragments arranged by size:
4576 2012 1074 1013 907 360 314 304
245
NaeI GCC'GGC
Cuts at: 6887 8986 6887
Size: 2099 8706
NarI GG'CG_CC
Cuts at: 8236 8484 8236
Size: 248 10557
NcoI C'CATG_G
Cuts at: 3131 3344 3131
Size: 213 10592
NdeI CA'TA_TG
Cuts at: 7437 7437
Size: 10805
NgoMIV G'CCGG_C
Cuts at: 6885 8984 6885
Size: 2099 8706
NsiI A_TGCA'T
Cuts at: 2251 3162 2251
Size: 911 9894
NspI r_CATG'y
Cuts at: 2249 2253 2319 2654 2878 3076 3844 4655 4756
Size: 4 66 335 224 198 768 811 101
Cuts at: 4756 6793 7013 7752 7858 8358 9265 2249
Size: 2037 220 739 106 500 907 3789
Fragments arranged by size:
3789 2037 907 811 768 739 500 335
224 220 198 106 101 66 4
PciI A'CATG_T
Cuts at: 3072 3840 6789 7009 9261 3072
Size: 768 2949 220 2252 4616
Fragments arranged by size:
4616 2949 2252 768 220
PflMI CCAn_nnn'nTGG
Cuts at: 6828 6828
Size: 10805
PfoI T'CCnGG_A
Cuts at: 4047 6143 4047
Size: 2096 8709
PmeI GTTT'AAAC
Cuts at: 7804 7804
Size: 10805
PmlI CAC'GTG
Cuts at: 3997 3997
Size: 10805
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5377 5409 9944 9976 5377
Size: 32 4535 32 6206
Fragments arranged by size:
6206 4535 32 32
PpuMI rG'GwC_Cy
Cuts at: 4473 7692 4473
Size: 3219 7586
PshAI GACnn'nnGTC
Cuts at: 1731 5605 1731
Size: 3874 6931
PsiI TTA'TAA
Cuts at: 838 1772 2029 5509 5758 6578 838
Size: 934 257 3480 249 820 5065
Fragments arranged by size:
5065 3480 934 820 257 249
PspOMI G'GGCC_C
Cuts at: 4444 4444
Size: 10805
PspXI vC'TCGA_Gb
Cuts at: 4880 4880
Size: 10805
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2670 2702 2841 2873 2670
Size: 32 139 32 10602
Fragments arranged by size:
10602 139 32 32
PstI C_TGCA'G
Cuts at: 122 489 7852 8537 122
Size: 367 7363 685 2390
Fragments arranged by size:
7363 2390 685 367
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10805
PvuII CAG'CTG
Cuts at: 117 421 4997 5904 8230 8590 117
Size: 304 4576 907 2326 360 2332
Fragments arranged by size:
4576 2332 2326 907 360 304
RsrII CG'GwC_CG
Cuts at: 9000 9000
Size: 10805
SacI G_AGCT'C
Cuts at: 1171 1420 3872 4638 7819 1171
Size: 249 2452 766 3181 4157
Fragments arranged by size:
4157 3181 2452 766 249
SalI G'TCGA_C
Cuts at: 7842 7842
Size: 10805
SanDI GG'GwC_CC
Cuts at: 4473 4473
Size: 10805
SapI GCTCTTCn'nnn_
Cuts at: 3234 3701 3860 8828 9038 3234
Size: 467 159 4968 210 5001
Fragments arranged by size:
5001 4968 467 210 159
SbfI CC_TGCA'GG
Cuts at: 7852 7852
Size: 10805
ScaI AGT'ACT
Cuts at: 5567 6964 7675 10322 5567
Size: 1397 711 2647 6050
Fragments arranged by size:
6050 2647 1397 711
SexAI A'CCwGG_T
Cuts at: 5306 6612 5306
Size: 1306 9499
SfcI C'TryA_G
Cuts at: 33 118 485 653 3203 5855 6458 6746 7848
Size: 85 367 168 2550 2652 603 288 1102
Cuts at: 7848 8533 9526 9717 33
Size: 685 993 191 1121
Fragments arranged by size:
2652 2550 1121 1102 993 685 603 367
288 191 168 85
SfoI GGC'GCC
Cuts at: 8237 8485 8237
Size: 248 10557
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10805
SmaI CCC'GGG
Cuts at: 7827 7827
Size: 10805
SmlI C'TyrA_G
Cuts at: 1515 2993 3896 4076 4637 4797 4880 6980 7301
Size: 1478 903 180 561 160 83 2100 321
Cuts at: 7301 7399 7960 8875 9367 9629 1515
Size: 98 561 915 492 262 2691
Fragments arranged by size:
2691 2100 1478 915 903 561 561 492
321 262 180 160 98 83
SpeI A'CTAG_T
Cuts at: 67 2683 67
Size: 2616 8189
SphI G_CATG'C
Cuts at: 2249 2253 2319 2878 4756 7858 8358 2249
Size: 4 66 559 1878 3102 500 4696
Fragments arranged by size:
4696 3102 1878 559 500 66 4
SspI AAT'ATT
Cuts at: 917 1237 2277 2541 4843 917
Size: 320 1040 264 2302 6879
Fragments arranged by size:
6879 2302 1040 320 264
StuI AGG'CCT
Cuts at: 6631 7459 6631
Size: 828 9977
StyI C'CwwG_G
Cuts at: 3131 3344 6731 7727 7939 8293 3131
Size: 213 3387 996 212 354 5643
Fragments arranged by size:
5643 3387 996 354 213 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3671 6696 8388 8521 8980 9189 3671
Size: 3025 1692 133 459 209 5287
Fragments arranged by size:
5287 3025 1692 459 209 133
TatI w'GTAC_w
Cuts at: 1831 2491 3616 4609 5565 6849 6951 6962 7065
Size: 660 1125 993 956 1284 102 11 103
Cuts at: 7065 7673 7733 7745 10320 1831
Size: 608 60 12 2575 2316
Fragments arranged by size:
2575 2316 1284 1125 993 956 660 608
103 102 60 12 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2765 2797 3059 3091 4479 4511 5383 5415 5667
Size: 32 262 32 1388 32 872 32 252
Cuts at: 5667 5699 5706 5738 6682 6714 6844 6876 7110
Size: 32 7 32 944 32 130 32 234
Cuts at: 7110 7142 7684 7716 10322 10354 2765
Size: 32 542 32 2606 32 3216
Fragments arranged by size:
3216 2606 1388 944 872 542 262 252
234 130 32 32 32 32 32 32
32 32 32 32 32 7
Tth111I GACn'n_nGTC
Cuts at: 8602 8602
Size: 10805
XbaI T'CTAG_A
Cuts at: 1345 7836 1345
Size: 6491 4314
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 573 3752 6141 6684 8300 573
Size: 3179 2389 543 1616 3078
Fragments arranged by size:
3179 3078 2389 1616 543
XhoI C'TCGA_G
Cuts at: 4880 7301 4880
Size: 2421 8384
XmaI C'CCGG_G
Cuts at: 7825 7825
Size: 10805
XmnI GAAnn'nnTTC
Cuts at: 980 5140 980
Size: 4160 6645
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AscI AvrII BcgI BglI BmgBI
BmtI BsiWI BssHII BstZ17I CspCI DraIII FseI FspAI
NheI NotI NruI PacI PasI SacII SfiI SgrAI
SnaBI SrfI SwaI ZraI