Restriction map for
pF1KE2548
(Circular) (Six-base) MAPSORT of: pF1KE2548.seq Check: 1466 from: 1 to: 5933
pF1KE2548 5933 bp
With 182 enzymes: SgfI *
October 24, 2014 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1243 1243
Size: 5933
AatII G_ACGT'C
Cuts at: 1356 1356
Size: 5933
AccI GT'mk_AC
Cuts at: 1742 2132 2971 1742
Size: 390 839 4704
Fragments arranged by size:
4704 839 390
Acc65I G'GTAC_C
Cuts at: 2949 2949
Size: 5933
AclI AA'CG_TT
Cuts at: 693 5773 693
Size: 5080 853
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 1099 2074 2200 2710 2875 3758 4190 4937
Size: 636 975 126 510 165 883 432 747
Cuts at: 4937 5205 463
Size: 268 1191
Fragments arranged by size:
1191 975 883 747 636 510 432 268
165 126
AflIII A'CryG_T
Cuts at: 2171 3285 4389 5366 2171
Size: 1114 1104 977 2738
Fragments arranged by size:
2738 1114 1104 977
AgeI A'CCGG_T
Cuts at: 4321 4321
Size: 5933
AlwNI CAG_nnn'CTG
Cuts at: 1773 2853 3238 4805 1773
Size: 1080 385 1567 2901
Fragments arranged by size:
2901 1567 1080 385
ApaI G_GGCC'C
Cuts at: 1321 2708 2867 1321
Size: 1387 159 4387
Fragments arranged by size:
4387 1387 159
ApaLI G'TGCA_C
Cuts at: 1750 2149 4703 1750
Size: 399 2554 2980
Fragments arranged by size:
2980 2554 399
ApoI r'AATT_y
Cuts at: 2554 2937 5286 5542 2554
Size: 383 2349 256 2945
Fragments arranged by size:
2945 2349 383 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5933
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5933
AvaI C'yCGr_G
Cuts at: 845 983 2089 2953 845
Size: 138 1106 864 3825
Fragments arranged by size:
3825 1106 864 138
BamHI G'GATC_C
Cuts at: 1131 2958 1131
Size: 1827 4106
BanI G'GyrC_C
Cuts at: 1231 2358 2949 3363 3611 3646 1231
Size: 1127 591 414 248 35 3518
Fragments arranged by size:
3518 1127 591 414 248 35
BanII G_rGCy'C
Cuts at: 1032 1321 1448 1945 2273 2640 2708 2867 2947
Size: 289 127 497 328 367 68 159 80
Cuts at: 2947 3977 1032
Size: 1030 2988
Fragments arranged by size:
2988 1030 497 367 328 289 159 127
80 68
BbeI G_GCGC'C
Cuts at: 3367 3615 3367
Size: 248 5685
BbsI GAAGACnn'nnnn_
Cuts at: 473 634 1976 2878 473
Size: 161 1342 902 3528
Fragments arranged by size:
3528 1342 902 161
BbvCI CC'TCA_GC
Cuts at: 385 385
Size: 5933
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2080 2114 2080
Size: 34 5899
BciVI GTATCCnnnnn_n'
Cuts at: 2530 2795 3831 4592 5933 2530
Size: 265 1036 761 1341 2530
Fragments arranged by size:
2530 1341 1036 761 265
BclI T'GATC_A
Cuts at: 3453 3453
Size: 5933
BglI GCCn_nnn'nGGC
Cuts at: 2306 2306
Size: 5933
BglII A'GATC_T
Cuts at: 789 3448 5485 789
Size: 2659 2037 1237
Fragments arranged by size:
2659 2037 1237
BlpI GC'TnA_GC
Cuts at: 2267 3045 2267
Size: 778 5155
Bme1580I G_kGCm'C
Cuts at: 1234 1321 1754 2153 2363 2708 2867 3558 3651
Size: 87 433 399 210 345 159 691 93
Cuts at: 3651 4707 1234
Size: 1056 2460
Fragments arranged by size:
2460 1056 691 433 399 345 210 159
93 87
BmgBI CAC'GTC
Cuts at: 546 2565 546
Size: 2019 3914
BmrI ACTGGGnnnn_n'
Cuts at: 1861 2332 3145 3226 3561 5705 1861
Size: 471 813 81 335 2144 2089
Fragments arranged by size:
2144 2089 813 471 335 81
BmtI G_CTAG'C
Cuts at: 751 751
Size: 5933
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2767 4300 2767
Size: 1533 4400
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 679 1284 1368 1434 1542 2121 679
Size: 605 84 66 108 579 4491
Fragments arranged by size:
4491 605 579 108 84 66
Bpu10I CC'TnA_GC
Cuts at: 385 1226 385
Size: 841 5092
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 409 1596 1947 2768 3109 3988 4480 4778
Size: 63 1187 351 821 341 879 492 298
Cuts at: 4778 346
Size: 1501
Fragments arranged by size:
1501 1187 879 821 492 351 341 298
63
BsaI GGTCTCn'nnnn_
Cuts at: 1915 1923 5637 1915
Size: 8 3714 2211
Fragments arranged by size:
3714 2211 8
BsaAI yAC'GTr
Cuts at: 2174 3286 2174
Size: 1112 4821
BsaBI GATnn'nnATC
Cuts at: 6 3447 5484 6
Size: 3441 2037 455
Fragments arranged by size:
3441 2037 455
BsaHI Gr'CG_yC
Cuts at: 1353 3364 3612 4314 5810 1353
Size: 2011 248 702 1496 1476
Fragments arranged by size:
2011 1496 1476 702 248
BsaWI w'CCGG_w
Cuts at: 3122 3643 4321 4595 4742 5153 3122
Size: 521 678 274 147 411 3902
Fragments arranged by size:
3902 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1007 1037 1566 1596 1714 1744 2114 2144 1007
Size: 30 529 30 118 30 370 30 4796
Fragments arranged by size:
4796 529 370 118 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 211 214 217 220 1134 1287 1417 1514 2338
Size: 3 3 3 914 153 130 97 824
Cuts at: 2338 2782 211
Size: 444 3362
Fragments arranged by size:
3362 914 824 444 153 130 97 3
3 3
BseYI C'CCAG_C
Cuts at: 537 819 1346 1540 1835 2273 2398 3358 4116
Size: 282 527 194 295 438 125 960 758
Cuts at: 4116 4693 5189 537
Size: 577 496 1281
Fragments arranged by size:
1281 960 758 577 527 496 438 295
282 194 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1668 1668
Size: 5933
BsiEI CG_ry'CG
Cuts at: 104 128 3475 3521 4729 104
Size: 24 3347 46 1208 1308
Fragments arranged by size:
3347 1308 1208 46 24
BsiHKAI G_wGCw'C
Cuts at: 1448 1754 2153 2250 2570 2947 3725 3915 4707
Size: 306 399 97 320 377 778 190 792
Cuts at: 4707 1448
Size: 2674
Fragments arranged by size:
2674 792 778 399 377 320 306 190
97
BsmI GAATG_Cn'
Cuts at: 2160 2160
Size: 5933
Bsp1286I G_dGCh'C
Cuts at: 1032 1234 1321 1448 1754 1945 2153 2250 2273
Size: 202 87 127 306 191 208 97 23
Cuts at: 2273 2363 2570 2640 2708 2867 2947 3558 3651
Size: 90 207 70 68 159 80 611 93
Cuts at: 3651 3725 3915 3977 4707 1032
Size: 74 190 62 730 2258
Fragments arranged by size:
2258 730 611 306 208 207 202 191
190 159 127 97 93 90 87 80
74 70 68 62 23
BspEI T'CCGG_A
Cuts at: 5153 5153
Size: 5933
BspHI T'CATG_A
Cuts at: 5109 5109
Size: 5933
BspMI ACCTGCnnnn'nnnn_
Cuts at: 950 1243 2441 2983 3197 3499 3880 950
Size: 293 1198 542 214 302 381 3003
Fragments arranged by size:
3003 1198 542 381 302 293 214
BsrBI CCG'CTC
Cuts at: 50 1371 1522 4225 4279 5763 5928 50
Size: 1321 151 2703 54 1484 165 55
Fragments arranged by size:
2703 1484 1321 165 151 55 54
BsrDI GCAATG_nn'
Cuts at: 480 3845 480
Size: 3365 2568
BsrFI r'CCGG_y
Cuts at: 127 3931 4112 4321 127
Size: 3804 181 209 1739
Fragments arranged by size:
3804 1739 209 181
BssSI C'ACGA_G
Cuts at: 4204 4562 4204
Size: 358 5575
BstAPI GCAn_nnn'nTGC
Cuts at: 2157 3310 2157
Size: 1153 4780
BstBI TT'CG_AA
Cuts at: 4294 4294
Size: 5933
BstXI CCAn_nnnn'nTGG
Cuts at: 2805 2805
Size: 5933
BstYI r'GATC_y
Cuts at: 1 789 1131 2958 3425 3448 3783 4029 5041
Size: 788 342 1827 467 23 335 246 1012
Cuts at: 5041 5127 5139 5485 1
Size: 86 12 346 449
Fragments arranged by size:
1827 1012 788 467 449 346 342 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 403 403
Size: 5933
BtgI C'CryG_G
Cuts at: 107 139 2345 2505 107
Size: 32 2206 160 3535
Fragments arranged by size:
3535 2206 160 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 404 700 1667 3883 4064 4227 404
Size: 296 967 2216 181 163 2110
Fragments arranged by size:
2216 2110 967 296 181 163
BtsI GCAGTG_nn'
Cuts at: 2012 3176 2012
Size: 1164 4769
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2001 2036 2001
Size: 35 5898
DraI TTT'AAA
Cuts at: 2932 2932
Size: 5933
DraIII CAC_nnn'GTG
Cuts at: 1758 2451 1758
Size: 693 5240
DrdI GACnn_nn'nnGTC
Cuts at: 1837 3639 4497 1837
Size: 1802 858 3273
Fragments arranged by size:
3273 1802 858
EaeI y'GGCC_r
Cuts at: 125 332 1362 2342 2660 2795 3518 3692 4083
Size: 207 1030 980 318 135 723 174 391
Cuts at: 4083 4110 5455 125
Size: 27 1345 603
Fragments arranged by size:
1345 1030 980 723 603 391 318 207
174 135 27
EagI C'GGCC_G
Cuts at: 125 3518 125
Size: 3393 2540
EarI CTCTTCn'nnn_
Cuts at: 235 439 476 503 581 1252 1623 2395 2759
Size: 204 37 27 78 671 371 772 364
Cuts at: 2759 2760 3956 4166 235
Size: 1 1196 210 2002
Fragments arranged by size:
2002 1196 772 671 371 364 210 204
78 37 27 1
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4141 4451 4597 5743 4141
Size: 310 146 1146 4331
Fragments arranged by size:
4331 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1446 2945 1446
Size: 1499 4434
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 679 1099 1284 1368 1434 1542 2074 2121
Size: 216 420 185 84 66 108 532 47
Cuts at: 2121 2200 2710 2875 3758 4190 4937 5205 463
Size: 79 510 165 883 432 747 268 1191
Fragments arranged by size:
1191 883 747 532 510 432 420 268
216 185 165 108 84 79 66 47
EcoNI CCTnn'n_nnAGG
Cuts at: 427 427
Size: 5933
EcoO109I rG'GnC_Cy
Cuts at: 1317 1562 1587 3072 1317
Size: 245 25 1485 4178
Fragments arranged by size:
4178 1485 245 25
EcoRI G'AATT_C
Cuts at: 2554 2937 2554
Size: 383 5550
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2278 3952 2278
Size: 1674 4259
FspI TGC'GCA
Cuts at: 157 3714 157
Size: 3557 2376
HaeII r_GCGC'y
Cuts at: 3367 3615 4637 5616 3367
Size: 248 1022 979 3684
Fragments arranged by size:
3684 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1116 1148 2114 2146 2528 2560 2766 2798 3775
Size: 32 966 32 382 32 206 32 977
Cuts at: 3775 3805 3807 3837 1116
Size: 30 2 30 3212
Fragments arranged by size:
3212 977 966 382 206 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2972 2972
Size: 5933
HindIII A'AGCT_T
Cuts at: 1593 1593
Size: 5933
KasI G'GCGC_C
Cuts at: 3363 3611 3363
Size: 248 5685
KpnI G_GTAC'C
Cuts at: 2953 2953
Size: 5933
MluI A'CGCG_T
Cuts at: 5366 5366
Size: 5933
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4604 4788 4604
Size: 184 5749
MscI TGG'CCA
Cuts at: 334 2662 3694 334
Size: 2328 1032 2573
Fragments arranged by size:
2573 2328 1032
MslI CAynn'nnrTG
Cuts at: 2735 3922 4049 2735
Size: 1187 127 4619
Fragments arranged by size:
4619 1187 127
MspA1I CmG'CkG
Cuts at: 141 1430 1603 2098 2277 2427 3044 3358 3718
Size: 1289 173 495 179 150 617 314 360
Cuts at: 3718 4731 4976 141
Size: 1013 245 1098
Fragments arranged by size:
1289 1098 1013 617 495 360 314 245
179 173 150
NaeI GCC'GGC
Cuts at: 129 4114 129
Size: 3985 1948
NarI GG'CG_CC
Cuts at: 3364 3612 3364
Size: 248 5685
NcoI C'CATG_G
Cuts at: 107 2505 107
Size: 2398 3535
NgoMIV G'CCGG_C
Cuts at: 127 4112 127
Size: 3985 1948
NheI G'CTAG_C
Cuts at: 747 747
Size: 5933
NspI r_CATG'y
Cuts at: 799 2986 3486 4393 799
Size: 2187 500 907 2339
Fragments arranged by size:
2339 2187 907 500
PciI A'CATG_T
Cuts at: 4389 4389
Size: 5933
PfoI T'CCnGG_A
Cuts at: 1134 1558 1134
Size: 424 5509
PmeI GTTT'AAAC
Cuts at: 2932 2932
Size: 5933
PmlI CAC'GTG
Cuts at: 2174 2174
Size: 5933
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5072 5104 5072
Size: 32 5901
PpuMI rG'GwC_Cy
Cuts at: 1562 1587 1562
Size: 25 5908
PsiI TTA'TAA
Cuts at: 370 1397 370
Size: 1027 4906
PspOMI G'GGCC_C
Cuts at: 1317 2704 2863 1317
Size: 1387 159 4387
Fragments arranged by size:
4387 1387 159
PstI C_TGCA'G
Cuts at: 289 781 947 1037 1240 2980 3665 289
Size: 492 166 90 203 1740 685 2557
Fragments arranged by size:
2557 1740 685 492 203 166 90
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5933
PvuII CAG'CTG
Cuts at: 2098 2277 2427 3358 3718 2098
Size: 179 150 931 360 4313
Fragments arranged by size:
4313 931 360 179 150
RsrII CG'GwC_CG
Cuts at: 4128 4128
Size: 5933
SacI G_AGCT'C
Cuts at: 1448 2947 1448
Size: 1499 4434
SacII CC_GC'GG
Cuts at: 142 142
Size: 5933
SalI G'TCGA_C
Cuts at: 2970 2970
Size: 5933
SapI GCTCTTCn'nnn_
Cuts at: 235 1623 3956 4166 235
Size: 1388 2333 210 2002
Fragments arranged by size:
2333 2002 1388 210
SbfI CC_TGCA'GG
Cuts at: 1240 2980 1240
Size: 1740 4193
ScaI AGT'ACT
Cuts at: 5450 5450
Size: 5933
SexAI A'CCwGG_T
Cuts at: 875 875
Size: 5933
SfcI C'TryA_G
Cuts at: 33 285 777 943 1033 1063 1236 2976 3661
Size: 252 492 166 90 30 173 1740 685
Cuts at: 3661 4654 4845 33
Size: 993 191 1121
Fragments arranged by size:
1740 1121 993 685 492 252 191 173
166 90 30
SfoI GGC'GCC
Cuts at: 3365 3613 3365
Size: 248 5685
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5933
SmaI CCC'GGG
Cuts at: 2955 2955
Size: 5933
SmlI C'TyrA_G
Cuts at: 361 424 845 983 1611 1926 2783 3088 4003
Size: 63 421 138 628 315 857 305 915
Cuts at: 4003 4495 4757 361
Size: 492 262 1537
Fragments arranged by size:
1537 915 857 628 492 421 315 305
262 138 63
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5933
SphI G_CATG'C
Cuts at: 799 2986 3486 799
Size: 2187 500 3246
Fragments arranged by size:
3246 2187 500
StyI C'CwwG_G
Cuts at: 107 170 335 1112 2106 2505 3067 3421 107
Size: 63 165 777 994 399 562 354 2619
Fragments arranged by size:
2619 994 777 562 399 354 165 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 529 3516 3649 4108 4317 529
Size: 2987 133 459 209 2145
Fragments arranged by size:
2987 2145 459 209 133
TatI w'GTAC_w
Cuts at: 556 5448 556
Size: 4892 1041
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1554 1586 1714 1746 5450 5482 1554
Size: 32 128 32 3704 32 2005
Fragments arranged by size:
3704 2005 128 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1867 2476 3730 1867
Size: 609 1254 4070
Fragments arranged by size:
4070 1254 609
XbaI T'CTAG_A
Cuts at: 1167 2964 1167
Size: 1797 4136
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1809 2488 3428 1809
Size: 679 940 4314
Fragments arranged by size:
4314 940 679
XhoI C'TCGA_G
Cuts at: 845 983 845
Size: 138 5795
XmaI C'CCGG_G
Cuts at: 2953 2953
Size: 5933
XmnI GAAnn'nnTTC
Cuts at: 694 886 694
Size: 192 5741
ZraI GAC'GTC
Cuts at: 1354 1354
Size: 5933
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PciI
PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BfrBI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
ClaI EcoRV FseI FspAI HpaI MfeI NdeI NotI
NruI NsiI PacI PasI PflMI PshAI PspXI PsrI
SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI