(Circular) (Six-base) MAPSORT of: pF1KE2553.seq Check: 3722 from: 1 to: 3887 pF1KE2553 3887 bp With 182 enzymes: SgfI * December 15, 2014 12:34 .. AccI GT'mk_AC Cuts at: 925 925 Size: 3887 Acc65I G'GTAC_C Cuts at: 903 903 Size: 3887 AclI AA'CG_TT Cuts at: 3727 3727 Size: 3887 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 200 1712 2144 2891 3159 200 Size: 1512 432 747 268 928 Fragments arranged by size: 1512 928 747 432 268 AflIII A'CryG_T Cuts at: 458 1239 2343 3320 458 Size: 781 1104 977 1025 Fragments arranged by size: 1104 1025 977 781 AgeI A'CCGG_T Cuts at: 2275 2275 Size: 3887 AleI CACnn'nnGTG Cuts at: 461 461 Size: 3887 AlwNI CAG_nnn'CTG Cuts at: 1192 2759 1192 Size: 1567 2320 ApaLI G'TGCA_C Cuts at: 2657 2657 Size: 3887 ApoI r'AATT_y Cuts at: 668 891 3240 3496 668 Size: 223 2349 256 1059 Fragments arranged by size: 2349 1059 256 223 AseI AT'TA_AT Cuts at: 20 20 Size: 3887 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3887 AvaI C'yCGr_G Cuts at: 148 782 907 148 Size: 634 125 3128 Fragments arranged by size: 3128 634 125 BamHI G'GATC_C Cuts at: 912 912 Size: 3887 BanI G'GyrC_C Cuts at: 903 1317 1565 1600 903 Size: 414 248 35 3190 Fragments arranged by size: 3190 414 248 35 BanII G_rGCy'C Cuts at: 172 340 901 1931 172 Size: 168 561 1030 2128 Fragments arranged by size: 2128 1030 561 168 BbeI G_GCGC'C Cuts at: 1321 1569 1321 Size: 248 3639 BciVI GTATCCnnnnn_n' Cuts at: 1785 2546 3887 1785 Size: 761 1341 1785 Fragments arranged by size: 1785 1341 761 BclI T'GATC_A Cuts at: 1407 1407 Size: 3887 BglII A'GATC_T Cuts at: 640 711 1402 3439 640 Size: 71 691 2037 1088 Fragments arranged by size: 2037 1088 691 71 BlpI GC'TnA_GC Cuts at: 999 999 Size: 3887 Bme1580I G_kGCm'C Cuts at: 1512 1605 2661 1512 Size: 93 1056 2738 Fragments arranged by size: 2738 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 383 1099 1180 1515 3659 383 Size: 716 81 335 2144 611 Fragments arranged by size: 2144 716 611 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2254 2254 Size: 3887 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 487 1063 1942 2434 2732 487 Size: 576 879 492 298 1642 Fragments arranged by size: 1642 879 576 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3591 3591 Size: 3887 BsaAI yAC'GTr Cuts at: 1240 1240 Size: 3887 BsaBI GATnn'nnATC Cuts at: 6 1401 3438 6 Size: 1395 2037 455 Fragments arranged by size: 2037 1395 455 BsaHI Gr'CG_yC Cuts at: 1318 1566 2268 3764 1318 Size: 248 702 1496 1441 Fragments arranged by size: 1496 1441 702 248 BsaWI w'CCGG_w Cuts at: 1076 1597 2275 2549 2696 3107 1076 Size: 521 678 274 147 411 1856 Fragments arranged by size: 1856 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 333 333 Size: 3887 BseYI C'CCAG_C Cuts at: 1312 2070 2647 3143 1312 Size: 758 577 496 2056 Fragments arranged by size: 2056 758 577 496 BsiEI CG_ry'CG Cuts at: 104 528 1429 1475 2683 104 Size: 424 901 46 1208 1308 Fragments arranged by size: 1308 1208 901 424 46 BsiHKAI G_wGCw'C Cuts at: 901 1679 1869 2661 901 Size: 778 190 792 2127 Fragments arranged by size: 2127 792 778 190 BsmI GAATG_Cn' Cuts at: 638 638 Size: 3887 Bsp1286I G_dGCh'C Cuts at: 172 340 901 1512 1605 1679 1869 1931 2661 Size: 168 561 611 93 74 190 62 730 Cuts at: 2661 172 Size: 1398 Fragments arranged by size: 1398 730 611 561 190 168 93 74 62 BspEI T'CCGG_A Cuts at: 3107 3107 Size: 3887 BspHI T'CATG_A Cuts at: 3063 3063 Size: 3887 BspMI ACCTGCnnnn'nnnn_ Cuts at: 937 1151 1453 1834 937 Size: 214 302 381 2990 Fragments arranged by size: 2990 381 302 214 BsrBI CCG'CTC Cuts at: 50 2179 2233 3717 3882 50 Size: 2129 54 1484 165 55 Fragments arranged by size: 2129 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1799 1799 Size: 3887 BsrFI r'CCGG_y Cuts at: 154 1885 2066 2275 154 Size: 1731 181 209 1766 Fragments arranged by size: 1766 1731 209 181 BssSI C'ACGA_G Cuts at: 2158 2516 2158 Size: 358 3529 BstAPI GCAn_nnn'nTGC Cuts at: 1264 1264 Size: 3887 BstBI TT'CG_AA Cuts at: 2248 2248 Size: 3887 BstYI r'GATC_y Cuts at: 1 539 640 711 912 1379 1402 1737 1983 Size: 538 101 71 201 467 23 335 246 Cuts at: 1983 2995 3081 3093 3439 1 Size: 1012 86 12 346 449 Fragments arranged by size: 1012 538 467 449 346 335 246 201 101 86 71 23 12 Bsu36I CC'TnA_GG Cuts at: 435 435 Size: 3887 BtgI C'CryG_G Cuts at: 107 107 Size: 3887 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1837 2018 2181 1837 Size: 181 163 3543 Fragments arranged by size: 3543 181 163 BtsI GCAGTG_nn' Cuts at: 1130 1130 Size: 3887 DraI TTT'AAA Cuts at: 886 886 Size: 3887 DrdI GACnn_nn'nnGTC Cuts at: 1593 2451 1593 Size: 858 3029 EaeI y'GGCC_r Cuts at: 725 1472 1646 2037 2064 3409 725 Size: 747 174 391 27 1345 1203 Fragments arranged by size: 1345 1203 747 391 174 27 EagI C'GGCC_G Cuts at: 1472 1472 Size: 3887 EarI CTCTTCn'nnn_ Cuts at: 176 1910 2120 176 Size: 1734 210 1943 Fragments arranged by size: 1943 1734 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2095 2405 2551 3697 2095 Size: 310 146 1146 2285 Fragments arranged by size: 2285 1146 310 146 EcoICRI GAG'CTC Cuts at: 899 899 Size: 3887 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 200 1712 2144 2891 3159 200 Size: 1512 432 747 268 928 Fragments arranged by size: 1512 928 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 1026 1026 Size: 3887 EcoRI G'AATT_C Cuts at: 891 891 Size: 3887 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 498 1906 498 Size: 1408 2479 FspI TGC'GCA Cuts at: 1668 1668 Size: 3887 HaeII r_GCGC'y Cuts at: 1321 1569 2591 3570 1321 Size: 248 1022 979 1638 Fragments arranged by size: 1638 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 418 450 703 735 1729 1759 1761 1791 418 Size: 32 253 32 994 30 2 30 2514 Fragments arranged by size: 2514 994 253 32 32 30 30 2 HincII GTy'rAC Cuts at: 926 926 Size: 3887 HindIII A'AGCT_T Cuts at: 249 249 Size: 3887 KasI G'GCGC_C Cuts at: 1317 1565 1317 Size: 248 3639 KpnI G_GTAC'C Cuts at: 907 907 Size: 3887 MluI A'CGCG_T Cuts at: 458 3320 458 Size: 2862 1025 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2558 2742 2558 Size: 184 3703 MscI TGG'CCA Cuts at: 727 1648 727 Size: 921 2966 MslI CAynn'nnrTG Cuts at: 461 1876 2003 461 Size: 1415 127 2345 Fragments arranged by size: 2345 1415 127 MspA1I CmG'CkG Cuts at: 533 998 1312 1672 2685 2930 533 Size: 465 314 360 1013 245 1490 Fragments arranged by size: 1490 1013 465 360 314 245 NaeI GCC'GGC Cuts at: 156 2068 156 Size: 1912 1975 NarI GG'CG_CC Cuts at: 1318 1566 1318 Size: 248 3639 NcoI C'CATG_G Cuts at: 107 107 Size: 3887 NgoMIV G'CCGG_C Cuts at: 154 2066 154 Size: 1912 1975 NspI r_CATG'y Cuts at: 940 1440 2347 940 Size: 500 907 2480 Fragments arranged by size: 2480 907 500 PciI A'CATG_T Cuts at: 2343 2343 Size: 3887 PmeI GTTT'AAAC Cuts at: 886 886 Size: 3887 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3026 3058 3026 Size: 32 3855 PspXI vC'TCGA_Gb Cuts at: 148 148 Size: 3887 PstI C_TGCA'G Cuts at: 711 934 1619 711 Size: 223 685 2979 Fragments arranged by size: 2979 685 223 PvuI CG_AT'CG Cuts at: 104 104 Size: 3887 PvuII CAG'CTG Cuts at: 533 1312 1672 533 Size: 779 360 2748 Fragments arranged by size: 2748 779 360 RsrII CG'GwC_CG Cuts at: 2082 2082 Size: 3887 SacI G_AGCT'C Cuts at: 901 901 Size: 3887 SalI G'TCGA_C Cuts at: 924 924 Size: 3887 SapI GCTCTTCn'nnn_ Cuts at: 1910 2120 1910 Size: 210 3677 SbfI CC_TGCA'GG Cuts at: 934 934 Size: 3887 ScaI AGT'ACT Cuts at: 3404 3404 Size: 3887 SfcI C'TryA_G Cuts at: 33 707 930 1615 2608 2799 33 Size: 674 223 685 993 191 1121 Fragments arranged by size: 1121 993 685 674 223 191 SfoI GGC'GCC Cuts at: 1319 1567 1319 Size: 248 3639 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3887 SmaI CCC'GGG Cuts at: 784 909 784 Size: 125 3762 SmlI C'TyrA_G Cuts at: 148 502 1042 1957 2449 2711 148 Size: 354 540 915 492 262 1324 Fragments arranged by size: 1324 915 540 492 354 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3887 SphI G_CATG'C Cuts at: 940 1440 940 Size: 500 3387 StuI AGG'CCT Cuts at: 433 745 433 Size: 312 3575 StyI C'CwwG_G Cuts at: 107 1021 1375 107 Size: 914 354 2619 Fragments arranged by size: 2619 914 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 294 1470 1603 2062 2271 294 Size: 1176 133 459 209 1910 Fragments arranged by size: 1910 1176 459 209 133 TatI w'GTAC_w Cuts at: 3402 3402 Size: 3887 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3404 3436 3404 Size: 32 3855 Tth111I GACn'n_nGTC Cuts at: 1684 1684 Size: 3887 XbaI T'CTAG_A Cuts at: 162 918 162 Size: 756 3131 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1382 1382 Size: 3887 XhoI C'TCGA_G Cuts at: 148 148 Size: 3887 XmaI C'CCGG_G Cuts at: 782 907 782 Size: 125 3762 XmnI GAAnn'nnTTC Cuts at: 757 757 Size: 3887 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AlfI AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI ZraI