Restriction map for
pF1KE2556
(Circular) (Six-base) MAPSORT of: pF1KE2556.seq Check: 8246 from: 1 to: 6635
pF1KE2556 6635 bp
With 182 enzymes: SgfI *
January 9, 2015 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1779 1779
Size: 6635
AatII G_ACGT'C
Cuts at: 1385 1385
Size: 6635
AccI GT'mk_AC
Cuts at: 1289 1577 3673 1289
Size: 288 2096 4251
Fragments arranged by size:
4251 2096 288
Acc65I G'GTAC_C
Cuts at: 3651 3651
Size: 6635
AclI AA'CG_TT
Cuts at: 1921 6475 1921
Size: 4554 2081
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 282 410 499 583 980 2569 3259 4460 4892
Size: 128 89 84 397 1589 690 1201 432
Cuts at: 4892 5639 5907 282
Size: 747 268 1010
Fragments arranged by size:
1589 1201 1010 747 690 432 397 268
128 89 84
AflII C'TTAA_G
Cuts at: 2818 2818
Size: 6635
AflIII A'CryG_T
Cuts at: 890 3987 5091 6068 890
Size: 3097 1104 977 1457
Fragments arranged by size:
3097 1457 1104 977
AgeI A'CCGG_T
Cuts at: 5023 5023
Size: 6635
AhdI GACnn_n'nnGTC
Cuts at: 1764 1764
Size: 6635
AleI CACnn'nnGTG
Cuts at: 3431 3583 3431
Size: 152 6483
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 899 899
Size: 6635
AlwNI CAG_nnn'CTG
Cuts at: 273 305 709 3214 3609 3940 5507 273
Size: 32 404 2505 395 331 1567 1401
Fragments arranged by size:
2505 1567 1401 404 395 331 32
ApaI G_GGCC'C
Cuts at: 2283 2369 2283
Size: 86 6549
ApaLI G'TGCA_C
Cuts at: 1295 1903 5405 1295
Size: 608 3502 2525
Fragments arranged by size:
3502 2525 608
ApoI r'AATT_y
Cuts at: 604 2762 2813 3639 5988 6244 604
Size: 2158 51 826 2349 256 995
Fragments arranged by size:
2349 2158 995 826 256 51
AseI AT'TA_AT
Cuts at: 20 20
Size: 6635
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6635
AvaI C'yCGr_G
Cuts at: 253 1588 2282 2633 2696 3516 3655 253
Size: 1335 694 351 63 820 139 3233
Fragments arranged by size:
3233 1335 820 694 351 139 63
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1206 1239 1420 1453 1206
Size: 33 181 33 6388
Fragments arranged by size:
6388 181 33 33
BamHI G'GATC_C
Cuts at: 185 1271 3660 185
Size: 1086 2389 3160
Fragments arranged by size:
3160 2389 1086
BanI G'GyrC_C
Cuts at: 2100 2286 3281 3651 4065 4313 4348 2100
Size: 186 995 370 414 248 35 4387
Fragments arranged by size:
4387 995 414 370 248 186 35
BanII G_rGCy'C
Cuts at: 701 1251 1976 2036 2125 2283 2369 2387 2535
Size: 550 725 60 89 158 86 18 148
Cuts at: 2535 2606 3649 4679 701
Size: 71 1043 1030 2657
Fragments arranged by size:
2657 1043 1030 725 550 158 148 89
86 71 60 18
BbeI G_GCGC'C
Cuts at: 2104 2290 4069 4317 2104
Size: 186 1779 248 4422
Fragments arranged by size:
4422 1779 248 186
BbsI GAAGACnn'nnnn_
Cuts at: 968 1073 3329 968
Size: 105 2256 4274
Fragments arranged by size:
4274 2256 105
BbvCI CC'TCA_GC
Cuts at: 1325 3535 1325
Size: 2210 4425
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 6601
BciVI GTATCCnnnnn_n'
Cuts at: 1207 4533 5294 6635 1207
Size: 3326 761 1341 1207
Fragments arranged by size:
3326 1341 1207 761
BclI T'GATC_A
Cuts at: 573 4155 573
Size: 3582 3053
BfrBI ATG'CAT
Cuts at: 382 382
Size: 6635
BglI GCCn_nnn'nGGC
Cuts at: 1824 2051 1824
Size: 227 6408
BglII A'GATC_T
Cuts at: 4150 6187 4150
Size: 2037 4598
BlpI GC'TnA_GC
Cuts at: 543 2035 3747 543
Size: 1492 1712 3431
Fragments arranged by size:
3431 1712 1492
Bme1580I G_kGCm'C
Cuts at: 1202 1299 1907 2283 2369 4260 4353 5409 1202
Size: 97 608 376 86 1891 93 1056 2428
Fragments arranged by size:
2428 1891 1056 608 376 97 93 86
BmgBI CAC'GTC
Cuts at: 2397 2516 2397
Size: 119 6516
BmrI ACTGGGnnnn_n'
Cuts at: 3269 3847 3928 4263 6407 3269
Size: 578 81 335 2144 3497
Fragments arranged by size:
3497 2144 578 335 81
BmtI G_CTAG'C
Cuts at: 538 538
Size: 6635
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2561 5002 2561
Size: 2441 4194
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 2068 2401 2487 3603 295
Size: 1773 333 86 1116 3327
Fragments arranged by size:
3327 1773 1116 333 86
Bpu10I CC'TnA_GC
Cuts at: 779 1325 1648 3535 779
Size: 546 323 1887 3879
Fragments arranged by size:
3879 1887 546 323
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2047 2205 3313 3811 4690 5182 5480 2047
Size: 158 1108 498 879 492 298 3202
Fragments arranged by size:
3202 1108 879 498 492 298 158
BsaI GGTCTCn'nnnn_
Cuts at: 934 2985 6339 934
Size: 2051 3354 1230
Fragments arranged by size:
3354 2051 1230
BsaAI yAC'GTr
Cuts at: 1119 2299 3988 1119
Size: 1180 1689 3766
Fragments arranged by size:
3766 1689 1180
BsaBI GATnn'nnATC
Cuts at: 6 3339 3478 4149 6186 6
Size: 3333 139 671 2037 455
Fragments arranged by size:
3333 2037 671 455 139
BsaHI Gr'CG_yC
Cuts at: 1382 2101 2216 2287 3372 4066 4314 5016 6512
Size: 719 115 71 1085 694 248 702 1496
Cuts at: 6512 1382
Size: 1505
Fragments arranged by size:
1505 1496 1085 719 702 694 248 115
71
BsaWI w'CCGG_w
Cuts at: 3824 4345 5023 5297 5444 5855 3824
Size: 521 678 274 147 411 4604
Fragments arranged by size:
4604 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 981 1011 981
Size: 30 6605
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 952 1089 2185 2257 2260 2263 2281 2284 3121
Size: 137 1096 72 3 3 18 3 837
Cuts at: 3121 952
Size: 4466
Fragments arranged by size:
4466 1096 837 137 72 18 3 3
3
BseYI C'CCAG_C
Cuts at: 1109 2126 2131 2456 3436 4060 4818 5395 5891
Size: 1017 5 325 980 624 758 577 496
Cuts at: 5891 1109
Size: 1853
Fragments arranged by size:
1853 1017 980 758 624 577 496 325
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1987 2279 2356 3580 1987
Size: 292 77 1224 5042
Fragments arranged by size:
5042 1224 292 77
BsiEI CG_ry'CG
Cuts at: 104 1269 1628 2447 4177 4223 5431 104
Size: 1165 359 819 1730 46 1208 1308
Fragments arranged by size:
1730 1308 1208 1165 819 359 46
BsiHKAI G_wGCw'C
Cuts at: 238 1299 1604 1907 2848 3649 4427 4617 5409
Size: 1061 305 303 941 801 778 190 792
Cuts at: 5409 238
Size: 1464
Fragments arranged by size:
1464 1061 941 801 792 778 305 303
190
BsiWI C'GTAC_G
Cuts at: 1547 1547
Size: 6635
BsmBI CGTCTCn'nnnn_
Cuts at: 1676 1772 2118 2465 1676
Size: 96 346 347 5846
Fragments arranged by size:
5846 347 346 96
Bsp1286I G_dGCh'C
Cuts at: 238 701 1202 1251 1299 1604 1907 1976 2036
Size: 463 501 49 48 305 303 69 60
Cuts at: 2036 2125 2283 2369 2387 2535 2606 2848 3649
Size: 89 158 86 18 148 71 242 801
Cuts at: 3649 4260 4353 4427 4617 4679 5409 238
Size: 611 93 74 190 62 730 1464
Fragments arranged by size:
1464 801 730 611 501 463 305 303
242 190 158 148 93 89 86 74
71 69 62 60 49 48 18
BspEI T'CCGG_A
Cuts at: 5855 5855
Size: 6635
BspHI T'CATG_A
Cuts at: 1385 3476 5811 1385
Size: 2091 2335 2209
Fragments arranged by size:
2335 2209 2091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1046 1779 3685 3899 4201 4582 1046
Size: 733 1906 214 302 381 3099
Fragments arranged by size:
3099 1906 733 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1695 2276 4927 4981 6465 6630 50
Size: 1645 581 2651 54 1484 165 55
Fragments arranged by size:
2651 1645 1484 581 165 55 54
BsrDI GCAATG_nn'
Cuts at: 881 3095 4547 881
Size: 2214 1452 2969
Fragments arranged by size:
2969 2214 1452
BsrFI r'CCGG_y
Cuts at: 332 1796 2559 4633 4814 5023 332
Size: 1464 763 2074 181 209 1944
Fragments arranged by size:
2074 1944 1464 763 209 181
BsrGI T'GTAC_A
Cuts at: 725 3587 725
Size: 2862 3773
BssHII G'CGCG_C
Cuts at: 1987 2302 1987
Size: 315 6320
BssSI C'ACGA_G
Cuts at: 4906 5264 4906
Size: 358 6277
BstAPI GCAn_nnn'nTGC
Cuts at: 170 1956 4012 170
Size: 1786 2056 2793
Fragments arranged by size:
2793 2056 1786
BstBI TT'CG_AA
Cuts at: 4996 4996
Size: 6635
BstYI r'GATC_y
Cuts at: 1 185 1271 1592 3356 3660 4127 4150 4485
Size: 184 1086 321 1764 304 467 23 335
Cuts at: 4485 4731 5743 5829 5841 6187 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1764 1086 1012 467 449 346 335 321
304 246 184 86 23 12
BtgI C'CryG_G
Cuts at: 227 260 1565 1703 1943 2040 227
Size: 33 1305 138 240 97 4822
Fragments arranged by size:
4822 1305 240 138 97 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4585 4766 4929 4585
Size: 181 163 6291
Fragments arranged by size:
6291 181 163
BtsI GCAGTG_nn'
Cuts at: 112 1290 1632 3426 3438 3878 112
Size: 1178 342 1794 12 440 2869
Fragments arranged by size:
2869 1794 1178 440 342 12
ClaI AT'CG_AT
Cuts at: 2883 2883
Size: 6635
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1121 1156 1121
Size: 35 6600
DraI TTT'AAA
Cuts at: 3634 3634
Size: 6635
DraIII CAC_nnn'GTG
Cuts at: 1914 1914
Size: 6635
DrdI GACnn_nn'nnGTC
Cuts at: 4341 5199 4341
Size: 858 5777
EaeI y'GGCC_r
Cuts at: 240 1625 1798 2027 2193 3139 4220 4394 4785
Size: 1385 173 229 166 946 1081 174 391
Cuts at: 4785 4812 6157 240
Size: 27 1345 718
Fragments arranged by size:
1385 1345 1081 946 718 391 229 174
173 166 27
EagI C'GGCC_G
Cuts at: 1625 4220 1625
Size: 2595 4040
EarI CTCTTCn'nnn_
Cuts at: 758 2407 3098 3118 3347 4658 4868 758
Size: 1649 691 20 229 1311 210 2525
Fragments arranged by size:
2525 1649 1311 691 229 210 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2191 4843 5153 5299 6445 2191
Size: 2652 310 146 1146 2381
Fragments arranged by size:
2652 2381 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3647 3647
Size: 6635
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 282 295 410 499 583 980 2068 2401 2487
Size: 13 115 89 84 397 1088 333 86
Cuts at: 2487 2569 3259 3603 4460 4892 5639 5907 282
Size: 82 690 344 857 432 747 268 1010
Fragments arranged by size:
1088 1010 857 747 690 432 397 344
333 268 115 89 86 84 82 13
EcoNI CCTnn'n_nnAGG
Cuts at: 1557 2326 2714 3177 1557
Size: 769 388 463 5015
Fragments arranged by size:
5015 769 463 388
EcoO109I rG'GnC_Cy
Cuts at: 1488 2052 2279 2331 2366 3774 1488
Size: 564 227 52 35 1408 4349
Fragments arranged by size:
4349 1408 564 227 52 35
EcoRI G'AATT_C
Cuts at: 2813 3639 2813
Size: 826 5809
EcoRV GAT'ATC
Cuts at: 489 489
Size: 6635
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 524 2914 4654 524
Size: 2390 1740 2505
Fragments arranged by size:
2505 2390 1740
FspI TGC'GCA
Cuts at: 1742 4416 1742
Size: 2674 3961
HaeII r_GCGC'y
Cuts at: 150 333 1424 1573 2104 2290 4069 4317 5339
Size: 183 1091 149 531 186 1779 248 1022
Cuts at: 5339 6318 150
Size: 979 467
Fragments arranged by size:
1779 1091 1022 979 531 467 248 186
183 149
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1750 1782 2560 2592 3346 3378 3584 3616 4477
Size: 32 778 32 754 32 206 32 861
Cuts at: 4477 4507 4509 4539 1750
Size: 30 2 30 3846
Fragments arranged by size:
3846 861 778 754 206 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3674 3674
Size: 6635
HindIII A'AGCT_T
Cuts at: 1236 1236
Size: 6635
KasI G'GCGC_C
Cuts at: 2100 2286 4065 4313 2100
Size: 186 1779 248 4422
Fragments arranged by size:
4422 1779 248 186
KpnI G_GTAC'C
Cuts at: 3655 3655
Size: 6635
MluI A'CGCG_T
Cuts at: 6068 6068
Size: 6635
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 213 1249 1382 2192 2477 2528 2546 3449 5306
Size: 1036 133 810 285 51 18 903 1857
Cuts at: 5306 5490 213
Size: 184 1358
Fragments arranged by size:
1857 1358 1036 903 810 285 184 133
51 18
MscI TGG'CCA
Cuts at: 242 3141 4396 242
Size: 2899 1255 2481
Fragments arranged by size:
2899 2481 1255
MslI CAynn'nnrTG
Cuts at: 845 1148 2023 3431 3583 4624 4751 845
Size: 303 875 1408 152 1041 127 2729
Fragments arranged by size:
2729 1408 1041 875 303 152 127
MspA1I CmG'CkG
Cuts at: 369 438 1567 1624 1746 2001 2460 3746 4060
Size: 69 1129 57 122 255 459 1286 314
Cuts at: 4060 4420 5433 5678 369
Size: 360 1013 245 1326
Fragments arranged by size:
1326 1286 1129 1013 459 360 314 255
245 122 69 57
NaeI GCC'GGC
Cuts at: 4816 4816
Size: 6635
NarI GG'CG_CC
Cuts at: 2101 2287 4066 4314 2101
Size: 186 1779 248 4422
Fragments arranged by size:
4422 1779 248 186
NdeI CA'TA_TG
Cuts at: 1170 1170
Size: 6635
NgoMIV G'CCGG_C
Cuts at: 4814 4814
Size: 6635
NheI G'CTAG_C
Cuts at: 534 534
Size: 6635
NotI GC'GGCC_GC
Cuts at: 1625 1625
Size: 6635
NsiI A_TGCA'T
Cuts at: 384 384
Size: 6635
NspI r_CATG'y
Cuts at: 732 894 1501 3534 3688 4188 5095 732
Size: 162 607 2033 154 500 907 2272
Fragments arranged by size:
2272 2033 907 607 500 162 154
PciI A'CATG_T
Cuts at: 890 5091 890
Size: 4201 2434
PflMI CCAn_nnn'nTGG
Cuts at: 273 669 2046 3148 273
Size: 396 1377 1102 3760
Fragments arranged by size:
3760 1377 1102 396
PfoI T'CCnGG_A
Cuts at: 1925 2378 1925
Size: 453 6182
PmeI GTTT'AAAC
Cuts at: 3634 3634
Size: 6635
PmlI CAC'GTG
Cuts at: 2299 2299
Size: 6635
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5774 5806 5774
Size: 32 6603
PpuMI rG'GwC_Cy
Cuts at: 2331 2331
Size: 6635
PspOMI G'GGCC_C
Cuts at: 2279 2365 2279
Size: 86 6549
PspXI vC'TCGA_Gb
Cuts at: 253 2633 253
Size: 2380 4255
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1201 1233 1201
Size: 32 6603
PstI C_TGCA'G
Cuts at: 443 1751 1961 3433 3682 4367 443
Size: 1308 210 1472 249 685 2711
Fragments arranged by size:
2711 1472 1308 685 249 210
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6635
PvuII CAG'CTG
Cuts at: 369 438 1746 2001 4060 4420 369
Size: 69 1308 255 2059 360 2584
Fragments arranged by size:
2584 2059 1308 360 255 69
RsrII CG'GwC_CG
Cuts at: 4830 4830
Size: 6635
SacI G_AGCT'C
Cuts at: 3649 3649
Size: 6635
SacII CC_GC'GG
Cuts at: 1568 1568
Size: 6635
SalI G'TCGA_C
Cuts at: 3672 3672
Size: 6635
SapI GCTCTTCn'nnn_
Cuts at: 3118 4658 4868 3118
Size: 1540 210 4885
Fragments arranged by size:
4885 1540 210
SbfI CC_TGCA'GG
Cuts at: 3682 3682
Size: 6635
ScaI AGT'ACT
Cuts at: 6152 6152
Size: 6635
SexAI A'CCwGG_T
Cuts at: 454 454
Size: 6635
SfcI C'TryA_G
Cuts at: 33 439 1290 1554 1578 1653 1747 1957 3429
Size: 406 851 264 24 75 94 210 1472
Cuts at: 3429 3678 4363 5356 5547 33
Size: 249 685 993 191 1121
Fragments arranged by size:
1472 1121 993 851 685 406 264 249
210 191 94 75 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 2051 2051
Size: 6635
SfoI GGC'GCC
Cuts at: 2102 2288 4067 4315 2102
Size: 186 1779 248 4422
Fragments arranged by size:
4422 1779 248 186
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6635
SgrAI Cr'CCGG_yG
Cuts at: 332 332
Size: 6635
SmaI CCC'GGG
Cuts at: 2284 3657 2284
Size: 1373 5262
SmlI C'TyrA_G
Cuts at: 253 2062 2184 2633 2696 2818 3328 3790 4705
Size: 1809 122 449 63 122 510 462 915
Cuts at: 4705 5197 5459 253
Size: 492 262 1429
Fragments arranged by size:
1809 1429 915 510 492 462 449 262
122 122 63
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6635
SphI G_CATG'C
Cuts at: 1501 3688 4188 1501
Size: 2187 500 3948
Fragments arranged by size:
3948 2187 500
SrfI GCCC'GGGC
Cuts at: 2284 2284
Size: 6635
StuI AGG'CCT
Cuts at: 1392 3236 1392
Size: 1844 4791
StyI C'CwwG_G
Cuts at: 789 2387 3769 4123 789
Size: 1598 1382 354 3301
Fragments arranged by size:
3301 1598 1382 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 4218 4351 4810 5019 4218
Size: 133 459 209 5834
Fragments arranged by size:
5834 459 209 133
TatI w'GTAC_w
Cuts at: 725 3575 3587 6150 725
Size: 2850 12 2563 1210
Fragments arranged by size:
2850 2563 1210 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 889 921 981 1013 2711 2743 3378 3410 6152
Size: 32 60 32 1698 32 635 32 2742
Cuts at: 6152 6184 889
Size: 32 1340
Fragments arranged by size:
2742 1698 1340 635 60 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 4432 4432
Size: 6635
XbaI T'CTAG_A
Cuts at: 3666 3666
Size: 6635
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2335 3433 4130 2335
Size: 1098 697 4840
Fragments arranged by size:
4840 1098 697
XhoI C'TCGA_G
Cuts at: 253 2633 2696 253
Size: 2380 63 4192
Fragments arranged by size:
4192 2380 63
XmaI C'CCGG_G
Cuts at: 2282 3655 2282
Size: 1373 5262
XmnI GAAnn'nnTTC
Cuts at: 2813 2813
Size: 6635
ZraI GAC'GTC
Cuts at: 1383 1383
Size: 6635
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NsiI NspI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BsmI BstEII BstXI BstZ17I
Bsu36I FseI FspAI HpaI MfeI NcoI NruI PacI
PasI PshAI PsiI SanDI SnaBI SspI SwaI