Restriction map for
pF1KE2566
(Circular) (Six-base) MAPSORT of: pF1KE2566.seq Check: 9045 from: 1 to: 4823
pF1KE2566 4823 bp
With 182 enzymes: SgfI *
March 23, 2015 09:39 ..
AccI GT'mk_AC
Cuts at: 221 1861 221
Size: 1640 3183
Acc65I G'GTAC_C
Cuts at: 1839 1839
Size: 4823
AclI AA'CG_TT
Cuts at: 4663 4663
Size: 4823
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 461 634 1410 1495 2648 3080 3827 4095
Size: 136 173 776 85 1153 432 747 268
Cuts at: 4095 325
Size: 1053
Fragments arranged by size:
1153 1053 776 747 432 268 173 136
85
AflII C'TTAA_G
Cuts at: 882 1552 882
Size: 670 4153
AflIII A'CryG_T
Cuts at: 2175 3279 4256 2175
Size: 1104 977 2742
Fragments arranged by size:
2742 1104 977
AgeI A'CCGG_T
Cuts at: 3211 3211
Size: 4823
AhdI GACnn_n'nnGTC
Cuts at: 429 429
Size: 4823
AleI CACnn'nnGTG
Cuts at: 1714 1714
Size: 4823
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1330 1732 1330
Size: 402 4421
AlwNI CAG_nnn'CTG
Cuts at: 2128 3695 2128
Size: 1567 3256
ApaLI G'TGCA_C
Cuts at: 1717 3593 1717
Size: 1876 2947
ApoI r'AATT_y
Cuts at: 478 715 916 1827 4176 4432 478
Size: 237 201 911 2349 256 869
Fragments arranged by size:
2349 911 869 256 237 201
AseI AT'TA_AT
Cuts at: 20 20
Size: 4823
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4823
AvaI C'yCGr_G
Cuts at: 1843 1843
Size: 4823
BamHI G'GATC_C
Cuts at: 558 1848 558
Size: 1290 3533
BanI G'GyrC_C
Cuts at: 1708 1839 2253 2501 2536 1708
Size: 131 414 248 35 3995
Fragments arranged by size:
3995 414 248 131 35
BanII G_rGCy'C
Cuts at: 365 1786 1837 2867 365
Size: 1421 51 1030 2321
Fragments arranged by size:
2321 1421 1030 51
BbeI G_GCGC'C
Cuts at: 2257 2505 2257
Size: 248 4575
BbsI GAAGACnn'nnnn_
Cuts at: 375 375
Size: 4823
BbvCI CC'TCA_GC
Cuts at: 467 467
Size: 4823
BciVI GTATCCnnnnn_n'
Cuts at: 2721 3482 4823 2721
Size: 761 1341 2721
Fragments arranged by size:
2721 1341 761
BclI T'GATC_A
Cuts at: 410 2343 410
Size: 1933 2890
BglII A'GATC_T
Cuts at: 2338 4375 2338
Size: 2037 2786
BlpI GC'TnA_GC
Cuts at: 1935 1935
Size: 4823
Bme1580I G_kGCm'C
Cuts at: 1721 2448 2541 3597 1721
Size: 727 93 1056 2947
Fragments arranged by size:
2947 1056 727 93
BmgBI CAC'GTC
Cuts at: 426 426
Size: 4823
BmrI ACTGGGnnnn_n'
Cuts at: 2035 2116 2451 4595 2035
Size: 81 335 2144 2263
Fragments arranged by size:
2263 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3190 3190
Size: 4823
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1171 1192 1706 1171
Size: 21 514 4288
Fragments arranged by size:
4288 514 21
Bpu10I CC'TnA_GC
Cuts at: 467 467
Size: 4823
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 569 1772 1999 2878 3370 3668 569
Size: 1203 227 879 492 298 1724
Fragments arranged by size:
1724 1203 879 492 298 227
BsaI GGTCTCn'nnnn_
Cuts at: 4527 4527
Size: 4823
BsaAI yAC'GTr
Cuts at: 2176 2176
Size: 4823
BsaBI GATnn'nnATC
Cuts at: 6 2337 4374 6
Size: 2331 2037 455
Fragments arranged by size:
2331 2037 455
BsaHI Gr'CG_yC
Cuts at: 2254 2502 3204 4700 2254
Size: 248 702 1496 2377
Fragments arranged by size:
2377 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2012 2533 3211 3485 3632 4043 2012
Size: 521 678 274 147 411 2792
Fragments arranged by size:
2792 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1646 1646
Size: 4823
BseYI C'CCAG_C
Cuts at: 566 1582 2248 3006 3583 4079 566
Size: 1016 666 758 577 496 1310
Fragments arranged by size:
1310 1016 758 666 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1747 1747
Size: 4823
BsiEI CG_ry'CG
Cuts at: 104 2365 2411 3619 104
Size: 2261 46 1208 1308
Fragments arranged by size:
2261 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 365 1206 1721 1786 1837 2615 2805 3597 365
Size: 841 515 65 51 778 190 792 1591
Fragments arranged by size:
1591 841 792 778 515 190 65 51
BsmBI CGTCTCn'nnnn_
Cuts at: 190 190
Size: 4823
Bsp1286I G_dGCh'C
Cuts at: 365 1206 1721 1786 1837 2448 2541 2615 2805
Size: 841 515 65 51 611 93 74 190
Cuts at: 2805 2867 3597 365
Size: 62 730 1591
Fragments arranged by size:
1591 841 730 611 515 190 93 74
65 62 51
BspEI T'CCGG_A
Cuts at: 4043 4043
Size: 4823
BspHI T'CATG_A
Cuts at: 293 3999 293
Size: 3706 1117
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1873 2087 2389 2770 1873
Size: 214 302 381 3926
Fragments arranged by size:
3926 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3115 3169 4653 4818 50
Size: 3065 54 1484 165 55
Fragments arranged by size:
3065 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 494 739 781 2735 494
Size: 245 42 1954 2582
Fragments arranged by size:
2582 1954 245 42
BsrFI r'CCGG_y
Cuts at: 2821 3002 3211 2821
Size: 181 209 4433
Fragments arranged by size:
4433 209 181
BssSI C'ACGA_G
Cuts at: 1372 3094 3452 1372
Size: 1722 358 2743
Fragments arranged by size:
2743 1722 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2200 2200
Size: 4823
BstBI TT'CG_AA
Cuts at: 3184 3184
Size: 4823
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4823
BstYI r'GATC_y
Cuts at: 1 558 1848 2315 2338 2673 2919 3931 4017
Size: 557 1290 467 23 335 246 1012 86
Cuts at: 4017 4029 4375 1
Size: 12 346 449
Fragments arranged by size:
1290 1012 557 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 222 222
Size: 4823
Bsu36I CC'TnA_GG
Cuts at: 1336 1604 1336
Size: 268 4555
BtgI C'CryG_G
Cuts at: 809 809
Size: 4823
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2773 2954 3117 2773
Size: 181 163 4479
Fragments arranged by size:
4479 181 163
BtsI GCAGTG_nn'
Cuts at: 2066 2066
Size: 4823
DraI TTT'AAA
Cuts at: 1822 1822
Size: 4823
DrdI GACnn_nn'nnGTC
Cuts at: 2529 3387 2529
Size: 858 3965
EaeI y'GGCC_r
Cuts at: 2408 2582 2973 3000 4345 2408
Size: 174 391 27 1345 2886
Fragments arranged by size:
2886 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2408 2408
Size: 4823
EarI CTCTTCn'nnn_
Cuts at: 1429 1640 1809 2846 3056 1429
Size: 211 169 1037 210 3196
Fragments arranged by size:
3196 1037 211 210 169
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3031 3341 3487 4633 3031
Size: 310 146 1146 3221
Fragments arranged by size:
3221 1146 310 146
EcoICRI GAG'CTC
Cuts at: 363 1784 1835 363
Size: 1421 51 3351
Fragments arranged by size:
3351 1421 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 461 634 1171 1192 1410 1495 1706 2648
Size: 136 173 537 21 218 85 211 942
Cuts at: 2648 3080 3827 4095 325
Size: 432 747 268 1053
Fragments arranged by size:
1053 942 747 537 432 268 218 211
173 136 85 21
EcoNI CCTnn'n_nnAGG
Cuts at: 1574 1574
Size: 4823
EcoO109I rG'GnC_Cy
Cuts at: 1579 1962 1579
Size: 383 4440
EcoRI G'AATT_C
Cuts at: 715 916 1827 715
Size: 201 911 3711
Fragments arranged by size:
3711 911 201
EcoRV GAT'ATC
Cuts at: 1611 1611
Size: 4823
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1567 2842 1567
Size: 1275 3548
FspI TGC'GCA
Cuts at: 2604 2604
Size: 4823
HaeII r_GCGC'y
Cuts at: 2257 2505 3527 4506 2257
Size: 248 1022 979 2574
Fragments arranged by size:
2574 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 415 447 621 653 878 910 2665 2695 2697
Size: 32 174 32 225 32 1755 30 2
Cuts at: 2697 2727 415
Size: 30 2511
Fragments arranged by size:
2511 1755 225 174 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1862 1862
Size: 4823
KasI G'GCGC_C
Cuts at: 2253 2501 2253
Size: 248 4575
KpnI G_GTAC'C
Cuts at: 1843 1843
Size: 4823
MluI A'CGCG_T
Cuts at: 4256 4256
Size: 4823
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 703 1092 3494 3678 703
Size: 389 2402 184 1848
Fragments arranged by size:
2402 1848 389 184
MscI TGG'CCA
Cuts at: 2584 2584
Size: 4823
MslI CAynn'nnrTG
Cuts at: 112 499 1199 1714 2812 2939 112
Size: 387 700 515 1098 127 1996
Fragments arranged by size:
1996 1098 700 515 387 127
MspA1I CmG'CkG
Cuts at: 161 1934 2248 2608 3621 3866 161
Size: 1773 314 360 1013 245 1118
Fragments arranged by size:
1773 1118 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3004 3004
Size: 4823
NarI GG'CG_CC
Cuts at: 2254 2502 2254
Size: 248 4575
NcoI C'CATG_G
Cuts at: 809 809
Size: 4823
NgoMIV G'CCGG_C
Cuts at: 3002 3002
Size: 4823
NspI r_CATG'y
Cuts at: 1102 1876 2376 3283 1102
Size: 774 500 907 2642
Fragments arranged by size:
2642 907 774 500
PciI A'CATG_T
Cuts at: 3279 3279
Size: 4823
PfoI T'CCnGG_A
Cuts at: 1683 1683
Size: 4823
PmeI GTTT'AAAC
Cuts at: 1822 1822
Size: 4823
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3962 3994 3962
Size: 32 4791
PsiI TTA'TAA
Cuts at: 1664 1664
Size: 4823
PstI C_TGCA'G
Cuts at: 1232 1383 1870 2555 1232
Size: 151 487 685 3500
Fragments arranged by size:
3500 685 487 151
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4823
PvuII CAG'CTG
Cuts at: 2248 2608 2248
Size: 360 4463
RsrII CG'GwC_CG
Cuts at: 3018 3018
Size: 4823
SacI G_AGCT'C
Cuts at: 365 1786 1837 365
Size: 1421 51 3351
Fragments arranged by size:
3351 1421 51
SalI G'TCGA_C
Cuts at: 1860 1860
Size: 4823
SapI GCTCTTCn'nnn_
Cuts at: 2846 3056 2846
Size: 210 4613
SbfI CC_TGCA'GG
Cuts at: 1870 1870
Size: 4823
ScaI AGT'ACT
Cuts at: 179 4340 179
Size: 4161 662
SfcI C'TryA_G
Cuts at: 33 241 1228 1379 1866 2551 3544 3735 33
Size: 208 987 151 487 685 993 191 1121
Fragments arranged by size:
1121 993 987 685 487 208 191 151
SfoI GGC'GCC
Cuts at: 2255 2503 2255
Size: 248 4575
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4823
SmaI CCC'GGG
Cuts at: 1845 1845
Size: 4823
SmlI C'TyrA_G
Cuts at: 584 882 1552 1787 1978 2893 3385 3647 584
Size: 298 670 235 191 915 492 262 1760
Fragments arranged by size:
1760 915 670 492 298 262 235 191
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4823
SphI G_CATG'C
Cuts at: 1876 2376 1876
Size: 500 4323
SspI AAT'ATT
Cuts at: 528 528
Size: 4823
StyI C'CwwG_G
Cuts at: 809 1957 2311 809
Size: 1148 354 3321
Fragments arranged by size:
3321 1148 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2406 2539 2998 3207 2406
Size: 133 459 209 4022
Fragments arranged by size:
4022 459 209 133
TatI w'GTAC_w
Cuts at: 134 177 4338 134
Size: 43 4161 619
Fragments arranged by size:
4161 619 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1332 1364 4340 4372 1332
Size: 32 2976 32 1783
Fragments arranged by size:
2976 1783 32 32
Tth111I GACn'n_nGTC
Cuts at: 2620 2620
Size: 4823
XbaI T'CTAG_A
Cuts at: 574 1854 574
Size: 1280 3543
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1440 2318 1440
Size: 878 3945
XmaI C'CCGG_G
Cuts at: 1843 1843
Size: 4823
XmnI GAAnn'nnTTC
Cuts at: 986 1732 986
Size: 746 4077
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AloI ApaI AscI AvrII BaeI
BcgI BfrBI BglI BmtI BsaXI BsiWI BsmI BsrGI
BssHII BstEII ClaI CspCI DraIII FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PshAI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI