Restriction map for
pF1KE2577
(Circular) (Six-base) MAPSORT of: pF1KE2577.seq Check: 5339 from: 1 to: 4082
pF1KE2577 4082 bp
With 182 enzymes: SgfI *
June 12, 2015 15:10 ..
AccI GT'mk_AC
Cuts at: 218 1120 218
Size: 902 3180
Acc65I G'GTAC_C
Cuts at: 782 1098 782
Size: 316 3766
AclI AA'CG_TT
Cuts at: 3922 3922
Size: 4082
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1907 2339 3086 3354 1907
Size: 432 747 268 2635
Fragments arranged by size:
2635 747 432 268
AflIII A'CryG_T
Cuts at: 1434 2538 3515 1434
Size: 1104 977 2001
Fragments arranged by size:
2001 1104 977
AgeI A'CCGG_T
Cuts at: 2470 2470
Size: 4082
AlwNI CAG_nnn'CTG
Cuts at: 1387 2954 1387
Size: 1567 2515
ApaLI G'TGCA_C
Cuts at: 2852 2852
Size: 4082
ApoI r'AATT_y
Cuts at: 1086 3435 3691 1086
Size: 2349 256 1477
Fragments arranged by size:
2349 1477 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4082
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4082
AvaI C'yCGr_G
Cuts at: 866 901 1057 1102 866
Size: 35 156 45 3846
Fragments arranged by size:
3846 156 45 35
BamHI G'GATC_C
Cuts at: 1107 1107
Size: 4082
BanI G'GyrC_C
Cuts at: 359 782 1098 1512 1760 1795 359
Size: 423 316 414 248 35 2646
Fragments arranged by size:
2646 423 414 316 248 35
BanII G_rGCy'C
Cuts at: 901 1096 2126 901
Size: 195 1030 2857
Fragments arranged by size:
2857 1030 195
BbeI G_GCGC'C
Cuts at: 1516 1764 1516
Size: 248 3834
BciVI GTATCCnnnnn_n'
Cuts at: 1980 2741 4082 1980
Size: 761 1341 1980
Fragments arranged by size:
1980 1341 761
BclI T'GATC_A
Cuts at: 950 1034 1602 950
Size: 84 568 3430
Fragments arranged by size:
3430 568 84
BglI GCCn_nnn'nGGC
Cuts at: 318 838 318
Size: 520 3562
BglII A'GATC_T
Cuts at: 1597 3634 1597
Size: 2037 2045
BlpI GC'TnA_GC
Cuts at: 932 1194 932
Size: 262 3820
Bme1580I G_kGCm'C
Cuts at: 362 1707 1800 2856 362
Size: 1345 93 1056 1588
Fragments arranged by size:
1588 1345 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 956 1294 1375 1710 3854 956
Size: 338 81 335 2144 1184
Fragments arranged by size:
2144 1184 338 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 207 560 2449 207
Size: 353 1889 1840
Fragments arranged by size:
1889 1840 353
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 946 202
Size: 744 3338
Bpu10I CC'TnA_GC
Cuts at: 895 895
Size: 4082
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1258 2137 2629 2927 1258
Size: 879 492 298 2413
Fragments arranged by size:
2413 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3786 3786
Size: 4082
BsaAI yAC'GTr
Cuts at: 1435 1435
Size: 4082
BsaBI GATnn'nnATC
Cuts at: 6 1596 3633 6
Size: 1590 2037 455
Fragments arranged by size:
2037 1590 455
BsaHI Gr'CG_yC
Cuts at: 890 1513 1761 2463 3959 890
Size: 623 248 702 1496 1013
Fragments arranged by size:
1496 1013 702 623 248
BsaWI w'CCGG_w
Cuts at: 1271 1792 2470 2744 2891 3302 1271
Size: 521 678 274 147 411 2051
Fragments arranged by size:
2051 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 283 1040 1507 2265 2842 3338 283
Size: 757 467 758 577 496 1027
Fragments arranged by size:
1027 758 757 577 496 467
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 808 808
Size: 4082
BsiEI CG_ry'CG
Cuts at: 104 1624 1670 2878 104
Size: 1520 46 1208 1308
Fragments arranged by size:
1520 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 188 917 1096 1874 2064 2856 188
Size: 729 179 778 190 792 1414
Fragments arranged by size:
1414 792 778 729 190 179
BsmI GAATG_Cn'
Cuts at: 278 635 278
Size: 357 3725
Bsp1286I G_dGCh'C
Cuts at: 188 362 901 917 1096 1707 1800 1874 2064
Size: 174 539 16 179 611 93 74 190
Cuts at: 2064 2126 2856 188
Size: 62 730 1414
Fragments arranged by size:
1414 730 611 539 190 179 174 93
74 62 16
BspEI T'CCGG_A
Cuts at: 3302 3302
Size: 4082
BspHI T'CATG_A
Cuts at: 3258 3258
Size: 4082
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1132 1346 1648 2029 1132
Size: 214 302 381 3185
Fragments arranged by size:
3185 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2374 2428 3912 4077 50
Size: 2324 54 1484 165 55
Fragments arranged by size:
2324 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 294 1994 294
Size: 1700 2382
BsrFI r'CCGG_y
Cuts at: 695 832 2080 2261 2470 695
Size: 137 1248 181 209 2307
Fragments arranged by size:
2307 1248 209 181 137
BsrGI T'GTAC_A
Cuts at: 679 679
Size: 4082
BssSI C'ACGA_G
Cuts at: 2353 2711 2353
Size: 358 3724
BstAPI GCAn_nnn'nTGC
Cuts at: 707 912 1459 707
Size: 205 547 3330
Fragments arranged by size:
3330 547 205
BstBI TT'CG_AA
Cuts at: 2443 2443
Size: 4082
BstYI r'GATC_y
Cuts at: 1 1107 1574 1597 1932 2178 3190 3276 3288
Size: 1106 467 23 335 246 1012 86 12
Cuts at: 3288 3634 1
Size: 346 449
Fragments arranged by size:
1106 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4082
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 367 2032 2213 2376 367
Size: 1665 181 163 2073
Fragments arranged by size:
2073 1665 181 163
BtsI GCAGTG_nn'
Cuts at: 1325 1325
Size: 4082
ClaI AT'CG_AT
Cuts at: 686 686
Size: 4082
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 831 866 831
Size: 35 4047
DraI TTT'AAA
Cuts at: 627 1081 627
Size: 454 3628
DrdI GACnn_nn'nnGTC
Cuts at: 1788 2646 1788
Size: 858 3224
EaeI y'GGCC_r
Cuts at: 346 855 1010 1667 1841 2232 2259 3604 346
Size: 509 155 657 174 391 27 1345 824
Fragments arranged by size:
1345 824 657 509 391 174 155 27
EagI C'GGCC_G
Cuts at: 1667 1667
Size: 4082
EarI CTCTTCn'nnn_
Cuts at: 2105 2315 2105
Size: 210 3872
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2290 2600 2746 3892 2290
Size: 310 146 1146 2480
Fragments arranged by size:
2480 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1094 1094
Size: 4082
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 946 1907 2339 3086 3354 202
Size: 744 961 432 747 268 930
Fragments arranged by size:
961 930 747 744 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 925 925
Size: 4082
EcoO109I rG'GnC_Cy
Cuts at: 1221 1221
Size: 4082
EcoRI G'AATT_C
Cuts at: 1086 1086
Size: 4082
EcoRV GAT'ATC
Cuts at: 583 583
Size: 4082
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2101 2101
Size: 4082
FspI TGC'GCA
Cuts at: 1863 1863
Size: 4082
HaeII r_GCGC'y
Cuts at: 1516 1764 2786 3765 1516
Size: 248 1022 979 1833
Fragments arranged by size:
1833 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 559 591 1924 1954 1956 1986 559
Size: 32 1333 30 2 30 2655
Fragments arranged by size:
2655 1333 32 30 30 2
HincII GTy'rAC
Cuts at: 1121 1121
Size: 4082
KasI G'GCGC_C
Cuts at: 1512 1760 1512
Size: 248 3834
KpnI G_GTAC'C
Cuts at: 786 1102 786
Size: 316 3766
MluI A'CGCG_T
Cuts at: 3515 3515
Size: 4082
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2753 2937 2753
Size: 184 3898
MscI TGG'CCA
Cuts at: 348 1843 348
Size: 1495 2587
MslI CAynn'nnrTG
Cuts at: 401 991 2071 2198 401
Size: 590 1080 127 2285
Fragments arranged by size:
2285 1080 590 127
MspA1I CmG'CkG
Cuts at: 822 1040 1193 1507 1867 2880 3125 822
Size: 218 153 314 360 1013 245 1779
Fragments arranged by size:
1779 1013 360 314 245 218 153
NaeI GCC'GGC
Cuts at: 834 2263 834
Size: 1429 2653
NarI GG'CG_CC
Cuts at: 1513 1761 1513
Size: 248 3834
NcoI C'CATG_G
Cuts at: 107 107
Size: 4082
NgoMIV G'CCGG_C
Cuts at: 832 2261 832
Size: 1429 2653
NspI r_CATG'y
Cuts at: 1135 1635 2542 1135
Size: 500 907 2675
Fragments arranged by size:
2675 907 500
PciI A'CATG_T
Cuts at: 2538 2538
Size: 4082
PflMI CCAn_nnn'nTGG
Cuts at: 355 355
Size: 4082
PfoI T'CCnGG_A
Cuts at: 763 923 763
Size: 160 3922
PmeI GTTT'AAAC
Cuts at: 1081 1081
Size: 4082
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3221 3253 3221
Size: 32 4050
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 440 472 440
Size: 32 4050
PstI C_TGCA'G
Cuts at: 163 565 1129 1814 163
Size: 402 564 685 2431
Fragments arranged by size:
2431 685 564 402
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4082
PvuII CAG'CTG
Cuts at: 822 1040 1507 1867 822
Size: 218 467 360 3037
Fragments arranged by size:
3037 467 360 218
RsrII CG'GwC_CG
Cuts at: 2277 2277
Size: 4082
SacI G_AGCT'C
Cuts at: 1096 1096
Size: 4082
SalI G'TCGA_C
Cuts at: 1119 1119
Size: 4082
SapI GCTCTTCn'nnn_
Cuts at: 2105 2315 2105
Size: 210 3872
SbfI CC_TGCA'GG
Cuts at: 1129 1129
Size: 4082
ScaI AGT'ACT
Cuts at: 3599 3599
Size: 4082
SfcI C'TryA_G
Cuts at: 33 159 252 561 1125 1810 2803 2994 33
Size: 126 93 309 564 685 993 191 1121
Fragments arranged by size:
1121 993 685 564 309 191 126 93
SfoI GGC'GCC
Cuts at: 1514 1762 1514
Size: 248 3834
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4082
SmaI CCC'GGG
Cuts at: 1059 1104 1059
Size: 45 4037
SmlI C'TyrA_G
Cuts at: 1237 2152 2644 2906 1237
Size: 915 492 262 2413
Fragments arranged by size:
2413 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4082
SphI G_CATG'C
Cuts at: 1135 1635 1135
Size: 500 3582
StuI AGG'CCT
Cuts at: 619 715 982 619
Size: 96 267 3719
Fragments arranged by size:
3719 267 96
StyI C'CwwG_G
Cuts at: 107 1216 1570 107
Size: 1109 354 2619
Fragments arranged by size:
2619 1109 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1665 1798 2257 2466 1665
Size: 133 459 209 3281
Fragments arranged by size:
3281 459 209 133
TatI w'GTAC_w
Cuts at: 679 3597 679
Size: 2918 1164
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 477 509 3599 3631 477
Size: 32 3090 32 928
Fragments arranged by size:
3090 928 32 32
Tth111I GACn'n_nGTC
Cuts at: 1879 1879
Size: 4082
XbaI T'CTAG_A
Cuts at: 1113 1113
Size: 4082
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 177 341 1577 177
Size: 164 1236 2682
Fragments arranged by size:
2682 1236 164
XmaI C'CCGG_G
Cuts at: 1057 1102 1057
Size: 45 4037
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI BsaXI BseRI BsiWI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I DraIII FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PpuMI PshAI PsiI PspOMI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI