Restriction map for
pF1KE2582
(Circular) (Six-base) MAPSORT of: pF1KE2582.seq Check: 5486 from: 1 to: 5981
pF1KE2582 5981 bp
With 182 enzymes: SgfI *
June 20, 2019 10:05 ..
AccI GT'mk_AC
Cuts at: 563 1573 3019 563
Size: 1010 1446 3525
Fragments arranged by size:
3525 1446 1010
Acc65I G'GTAC_C
Cuts at: 1196 2997 1196
Size: 1801 4180
AclI AA'CG_TT
Cuts at: 5821 5821
Size: 5981
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 693 877 886 1468 1519 3806 4238 4985 5253
Size: 184 9 582 51 2287 432 747 268
Cuts at: 5253 693
Size: 1421
Fragments arranged by size:
2287 1421 747 582 432 268 184 51
9
AflIII A'CryG_T
Cuts at: 3333 4437 5414 3333
Size: 1104 977 3900
Fragments arranged by size:
3900 1104 977
AgeI A'CCGG_T
Cuts at: 4369 4369
Size: 5981
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2614 2648 2944 2614
Size: 34 296 5651
Fragments arranged by size:
5651 296 34
AlwNI CAG_nnn'CTG
Cuts at: 411 1953 2227 2236 2509 3286 4853 411
Size: 1542 274 9 273 777 1567 1539
Fragments arranged by size:
1567 1542 1539 777 274 273 9
ApaI G_GGCC'C
Cuts at: 989 989
Size: 5981
ApaLI G'TGCA_C
Cuts at: 4751 4751
Size: 5981
ApoI r'AATT_y
Cuts at: 1541 1762 2985 5334 5590 1541
Size: 221 1223 2349 256 1932
Fragments arranged by size:
2349 1932 1223 256 221
AseI AT'TA_AT
Cuts at: 20 20
Size: 5981
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5981
AvaI C'yCGr_G
Cuts at: 951 1025 2938 3001 951
Size: 74 1913 63 3931
Fragments arranged by size:
3931 1913 74 63
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1027 1060 1027
Size: 33 5948
BamHI G'GATC_C
Cuts at: 2135 3006 2135
Size: 871 5110
BanI G'GyrC_C
Cuts at: 436 775 1196 2997 3411 3659 3694 436
Size: 339 421 1801 414 248 35 2723
Fragments arranged by size:
2723 1801 421 414 339 248 35
BanII G_rGCy'C
Cuts at: 989 1431 2293 2762 2995 4025 989
Size: 442 862 469 233 1030 2945
Fragments arranged by size:
2945 1030 862 469 442 233
BbeI G_GCGC'C
Cuts at: 3415 3663 3415
Size: 248 5733
BbsI GAAGACnn'nnnn_
Cuts at: 212 554 1641 1727 2458 2581 212
Size: 342 1087 86 731 123 3612
Fragments arranged by size:
3612 1087 731 342 123 86
BbvCI CC'TCA_GC
Cuts at: 650 1163 2555 2661 650
Size: 513 1392 106 3970
Fragments arranged by size:
3970 1392 513 106
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2416 2450 2416
Size: 34 5947
BciVI GTATCCnnnnn_n'
Cuts at: 3879 4640 5981 3879
Size: 761 1341 3879
Fragments arranged by size:
3879 1341 761
BclI T'GATC_A
Cuts at: 3501 3501
Size: 5981
BglI GCCn_nnn'nGGC
Cuts at: 2149 2149
Size: 5981
BglII A'GATC_T
Cuts at: 3496 5533 3496
Size: 2037 3944
BlpI GC'TnA_GC
Cuts at: 889 2803 3093 889
Size: 1914 290 3777
Fragments arranged by size:
3777 1914 290
Bme1580I G_kGCm'C
Cuts at: 778 989 2706 3606 3699 4755 778
Size: 211 1717 900 93 1056 2004
Fragments arranged by size:
2004 1717 1056 900 211 93
BmgBI CAC'GTC
Cuts at: 879 879
Size: 5981
BmrI ACTGGGnnnn_n'
Cuts at: 176 1069 2519 3193 3274 3609 5753 176
Size: 893 1450 674 81 335 2144 404
Fragments arranged by size:
2144 1450 893 674 404 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2646 4348 2646
Size: 1702 4279
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 937 2038 2167 2203 2317 2448 2890 937
Size: 1101 129 36 114 131 442 4028
Fragments arranged by size:
4028 1101 442 131 129 114 36
Bpu10I CC'TnA_GC
Cuts at: 650 1163 2555 2661 650
Size: 513 1392 106 3970
Fragments arranged by size:
3970 1392 513 106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 527 3157 4036 4528 4826 527
Size: 2630 879 492 298 1682
Fragments arranged by size:
2630 1682 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1649 5685 1649
Size: 4036 1945
BsaAI yAC'GTr
Cuts at: 3334 3334
Size: 5981
BsaBI GATnn'nnATC
Cuts at: 6 3495 5532 6
Size: 3489 2037 455
Fragments arranged by size:
3489 2037 455
BsaHI Gr'CG_yC
Cuts at: 3412 3660 4362 5858 3412
Size: 248 702 1496 3535
Fragments arranged by size:
3535 1496 702 248
BsaWI w'CCGG_w
Cuts at: 635 3170 3691 4369 4643 4790 5201 635
Size: 2535 521 678 274 147 411 1415
Fragments arranged by size:
2535 1415 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 316 346 1306 1336 2161 2191 316
Size: 30 960 30 825 30 4106
Fragments arranged by size:
4106 960 825 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 697 1151 1471 1912 2143 2200 2332 2335 2338
Size: 454 320 441 231 57 132 3 3
Cuts at: 2338 2451 697
Size: 113 4227
Fragments arranged by size:
4227 454 441 320 231 132 113 57
3 3
BseYI C'CCAG_C
Cuts at: 998 1384 1430 1958 2045 2097 2604 2770 2788
Size: 386 46 528 87 52 507 166 18
Cuts at: 2788 2841 3406 4164 4741 5237 998
Size: 53 565 758 577 496 1742
Fragments arranged by size:
1742 758 577 565 528 507 496 386
166 87 53 52 46 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 512 1297 2353 2914 512
Size: 785 1056 561 3579
Fragments arranged by size:
3579 1056 785 561
BsiEI CG_ry'CG
Cuts at: 104 2799 3523 3569 4777 104
Size: 2695 724 46 1208 1308
Fragments arranged by size:
2695 1308 1208 724 46
BsiHKAI G_wGCw'C
Cuts at: 1097 1359 2194 2293 2618 2762 2809 2995 3773
Size: 262 835 99 325 144 47 186 778
Cuts at: 3773 3963 4755 1097
Size: 190 792 2323
Fragments arranged by size:
2323 835 792 778 325 262 190 186
144 99 47
BsmI GAATG_Cn'
Cuts at: 2220 2220
Size: 5981
Bsp1286I G_dGCh'C
Cuts at: 778 989 1097 1359 1431 2194 2293 2618 2706
Size: 211 108 262 72 763 99 325 88
Cuts at: 2706 2762 2809 2995 3606 3699 3773 3963 4025
Size: 56 47 186 611 93 74 190 62
Cuts at: 4025 4755 778
Size: 730 2004
Fragments arranged by size:
2004 763 730 611 325 262 211 190
186 108 99 93 88 74 72 62
56 47
BspEI T'CCGG_A
Cuts at: 5201 5201
Size: 5981
BspHI T'CATG_A
Cuts at: 1641 5157 1641
Size: 3516 2465
BspMI ACCTGCnnnn'nnnn_
Cuts at: 997 3031 3245 3547 3928 997
Size: 2034 214 302 381 3050
Fragments arranged by size:
3050 2034 381 302 214
BsrBI CCG'CTC
Cuts at: 50 884 1998 2133 2483 4273 4327 5811 5976
Size: 834 1114 135 350 1790 54 1484 165
Cuts at: 5976 50
Size: 55
Fragments arranged by size:
1790 1484 1114 834 350 165 135 55
54
BsrDI GCAATG_nn'
Cuts at: 3893 3893
Size: 5981
BsrFI r'CCGG_y
Cuts at: 2798 3979 4160 4369 2798
Size: 1181 181 209 4410
Fragments arranged by size:
4410 1181 209 181
BssSI C'ACGA_G
Cuts at: 1135 4252 4610 1135
Size: 3117 358 2506
Fragments arranged by size:
3117 2506 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2227 3358 2227
Size: 1131 4850
BstBI TT'CG_AA
Cuts at: 4342 4342
Size: 5981
BstEII G'GTnAC_C
Cuts at: 1111 1111
Size: 5981
BstXI CCAn_nnnn'nTGG
Cuts at: 1123 1396 1123
Size: 273 5708
BstYI r'GATC_y
Cuts at: 1 312 350 1844 2135 3006 3473 3496 3831
Size: 311 38 1494 291 871 467 23 335
Cuts at: 3831 4077 5089 5175 5187 5533 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1494 1012 871 467 449 346 335 311
291 246 86 38 23 12
Bsu36I CC'TnA_GG
Cuts at: 346 346
Size: 5981
BtgI C'CryG_G
Cuts at: 527 753 2793 2886 527
Size: 226 2040 93 3622
Fragments arranged by size:
3622 2040 226 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3931 4112 4275 3931
Size: 181 163 5637
Fragments arranged by size:
5637 181 163
BtsI GCAGTG_nn'
Cuts at: 1309 1811 2225 2638 2706 3224 1309
Size: 502 414 413 68 518 4066
Fragments arranged by size:
4066 518 502 414 413 68
DraI TTT'AAA
Cuts at: 2980 2980
Size: 5981
DraIII CAC_nnn'GTG
Cuts at: 606 606
Size: 5981
DrdI GACnn_nn'nnGTC
Cuts at: 3687 4545 3687
Size: 858 5123
EaeI y'GGCC_r
Cuts at: 464 1184 2064 2796 2846 2883 3566 3740 4131
Size: 720 880 732 50 37 683 174 391
Cuts at: 4131 4158 5503 464
Size: 27 1345 942
Fragments arranged by size:
1345 942 880 732 720 683 391 174
50 37 27
EagI C'GGCC_G
Cuts at: 2796 3566 2796
Size: 770 5211
EarI CTCTTCn'nnn_
Cuts at: 1299 1889 2564 2767 4004 4214 1299
Size: 590 675 203 1237 210 3066
Fragments arranged by size:
3066 1237 675 590 210 203
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1287 4189 4499 4645 5791 1287
Size: 2902 310 146 1146 1477
Fragments arranged by size:
2902 1477 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2291 2760 2993 2291
Size: 469 233 5279
Fragments arranged by size:
5279 469 233
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 693 877 886 937 1468 1519 2038 2167 2203
Size: 184 9 51 531 51 519 129 36
Cuts at: 2203 2317 2448 2890 3806 4238 4985 5253 693
Size: 114 131 442 916 432 747 268 1421
Fragments arranged by size:
1421 916 747 531 519 442 432 268
184 131 129 114 51 51 36 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1852 1852
Size: 5981
EcoO109I rG'GnC_Cy
Cuts at: 158 985 986 1030 1950 2418 3120 158
Size: 827 1 44 920 468 702 3019
Fragments arranged by size:
3019 920 827 702 468 44 1
EcoRI G'AATT_C
Cuts at: 1762 2985 1762
Size: 1223 4758
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 334 4000 334
Size: 3666 2315
FspI TGC'GCA
Cuts at: 3762 3762
Size: 5981
HaeII r_GCGC'y
Cuts at: 856 3415 3663 4685 5664 856
Size: 2559 248 1022 979 1173
Fragments arranged by size:
2559 1173 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2505 2537 2802 2834 2900 2932 3823 3853 3855
Size: 32 265 32 66 32 891 30 2
Cuts at: 3855 3885 2505
Size: 30 4601
Fragments arranged by size:
4601 891 265 66 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3020 3020
Size: 5981
KasI G'GCGC_C
Cuts at: 3411 3659 3411
Size: 248 5733
KpnI G_GTAC'C
Cuts at: 1200 3001 1200
Size: 1801 4180
MluI A'CGCG_T
Cuts at: 5414 5414
Size: 5981
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2223 4652 4836 2223
Size: 2429 184 3368
Fragments arranged by size:
3368 2429 184
MscI TGG'CCA
Cuts at: 466 2066 2885 3742 466
Size: 1600 819 857 2705
Fragments arranged by size:
2705 1600 857 819
MslI CAynn'nnrTG
Cuts at: 2060 2624 3970 4097 2060
Size: 564 1346 127 3944
Fragments arranged by size:
3944 1346 564 127
MspA1I CmG'CkG
Cuts at: 391 408 909 1304 1962 2236 2550 2795 2845
Size: 17 501 395 658 274 314 245 50
Cuts at: 2845 3092 3406 3766 4779 5024 391
Size: 247 314 360 1013 245 1348
Fragments arranged by size:
1348 1013 658 501 395 360 314 314
274 247 245 245 50 17
NaeI GCC'GGC
Cuts at: 2800 4162 2800
Size: 1362 4619
NarI GG'CG_CC
Cuts at: 3412 3660 3412
Size: 248 5733
NcoI C'CATG_G
Cuts at: 753 2886 753
Size: 2133 3848
NdeI CA'TA_TG
Cuts at: 1920 1920
Size: 5981
NgoMIV G'CCGG_C
Cuts at: 2798 4160 2798
Size: 1362 4619
NspI r_CATG'y
Cuts at: 1976 2082 2629 3034 3534 4441 1976
Size: 106 547 405 500 907 3516
Fragments arranged by size:
3516 907 547 500 405 106
PasI CC'CwG_GG
Cuts at: 1123 1350 1123
Size: 227 5754
PciI A'CATG_T
Cuts at: 4437 4437
Size: 5981
PflMI CCAn_nnn'nTGG
Cuts at: 1140 1437 1140
Size: 297 5684
PfoI T'CCnGG_A
Cuts at: 416 1847 2105 416
Size: 1431 258 4292
Fragments arranged by size:
4292 1431 258
PmeI GTTT'AAAC
Cuts at: 2980 2980
Size: 5981
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5120 5152 5120
Size: 32 5949
PpuMI rG'GwC_Cy
Cuts at: 158 1030 2418 158
Size: 872 1388 3721
Fragments arranged by size:
3721 1388 872
PspOMI G'GGCC_C
Cuts at: 985 985
Size: 5981
PstI C_TGCA'G
Cuts at: 1008 1734 1946 1967 2232 2426 3028 3713 1008
Size: 726 212 21 265 194 602 685 3276
Fragments arranged by size:
3276 726 685 602 265 212 194 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5981
PvuII CAG'CTG
Cuts at: 391 909 1962 2236 2550 3406 3766 391
Size: 518 1053 274 314 856 360 2606
Fragments arranged by size:
2606 1053 856 518 360 314 274
RsrII CG'GwC_CG
Cuts at: 4176 4176
Size: 5981
SacI G_AGCT'C
Cuts at: 2293 2762 2995 2293
Size: 469 233 5279
Fragments arranged by size:
5279 469 233
SacII CC_GC'GG
Cuts at: 2796 2796
Size: 5981
SalI G'TCGA_C
Cuts at: 3018 3018
Size: 5981
SanDI GG'GwC_CC
Cuts at: 158 158
Size: 5981
SapI GCTCTTCn'nnn_
Cuts at: 2767 4004 4214 2767
Size: 1237 210 4534
Fragments arranged by size:
4534 1237 210
SbfI CC_TGCA'GG
Cuts at: 1008 3028 1008
Size: 2020 3961
ScaI AGT'ACT
Cuts at: 5498 5498
Size: 5981
SexAI A'CCwGG_T
Cuts at: 602 602
Size: 5981
SfcI C'TryA_G
Cuts at: 33 1004 1730 1942 1963 2228 2422 3024 3709
Size: 971 726 212 21 265 194 602 685
Cuts at: 3709 4702 4893 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 971 726 685 602 265 212
194 191 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 2149 2149
Size: 5981
SfoI GGC'GCC
Cuts at: 3413 3661 3413
Size: 248 5733
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5981
SmaI CCC'GGG
Cuts at: 3003 3003
Size: 5981
SmlI C'TyrA_G
Cuts at: 542 3136 4051 4543 4805 542
Size: 2594 915 492 262 1718
Fragments arranged by size:
2594 1718 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5981
SphI G_CATG'C
Cuts at: 2629 3034 3534 2629
Size: 405 500 5076
Fragments arranged by size:
5076 500 405
StyI C'CwwG_G
Cuts at: 753 2886 3115 3469 753
Size: 2133 229 354 3265
Fragments arranged by size:
3265 2133 354 229
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1342 2670 2855 3564 3697 4156 4365 1342
Size: 1328 185 709 133 459 209 2958
Fragments arranged by size:
2958 1328 709 459 209 185 133
TatI w'GTAC_w
Cuts at: 5496 5496
Size: 5981
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1306 1338 1803 1835 5498 5530 1306
Size: 32 465 32 3663 32 1757
Fragments arranged by size:
3663 1757 465 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3778 3778
Size: 5981
XbaI T'CTAG_A
Cuts at: 201 3012 201
Size: 2811 3170
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2053 2269 2547 3476 2053
Size: 216 278 929 4558
Fragments arranged by size:
4558 929 278 216
XmaI C'CCGG_G
Cuts at: 3001 3001
Size: 5981
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BfrBI BmtI BsiWI BsmBI BsrGI BssHII BstZ17I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NheI NotI NruI NsiI PacI PmlI PshAI PsiI
PspXI PsrI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI