Restriction map for
pF1KE2583
(Circular) (Six-base) MAPSORT of: pF1KE2583.seq Check: 8675 from: 1 to: 8039
pF1KE2583 8039 bp
With 182 enzymes: SgfI *
August 16, 2021 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2413 2413
Size: 8039
AccI GT'mk_AC
Cuts at: 1646 5077 1646
Size: 3431 4608
Acc65I G'GTAC_C
Cuts at: 5055 5055
Size: 8039
AclI AA'CG_TT
Cuts at: 7879 7879
Size: 8039
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2491 2893 4292 4781 5864 6296 7043 7311 2491
Size: 402 1399 489 1083 432 747 268 3219
Fragments arranged by size:
3219 1399 1083 747 489 432 402 268
AfeI AGC'GCT
Cuts at: 4023 4023
Size: 8039
AflIII A'CryG_T
Cuts at: 5391 6495 7472 5391
Size: 1104 977 5958
Fragments arranged by size:
5958 1104 977
AgeI A'CCGG_T
Cuts at: 6427 6427
Size: 8039
AhdI GACnn_n'nnGTC
Cuts at: 1089 1089
Size: 8039
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2431 2431
Size: 8039
AlwNI CAG_nnn'CTG
Cuts at: 2418 2614 5026 5344 6911 2418
Size: 196 2412 318 1567 3546
Fragments arranged by size:
3546 2412 1567 318 196
ApaI G_GGCC'C
Cuts at: 997 1412 997
Size: 415 7624
ApaLI G'TGCA_C
Cuts at: 6809 6809
Size: 8039
ApoI r'AATT_y
Cuts at: 403 2518 2689 5043 7392 7648 403
Size: 2115 171 2354 2349 256 794
Fragments arranged by size:
2354 2349 2115 794 256 171
AseI AT'TA_AT
Cuts at: 20 3302 20
Size: 3282 4757
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8039
AvaI C'yCGr_G
Cuts at: 3965 4553 5059 3965
Size: 588 506 6945
Fragments arranged by size:
6945 588 506
AvrII C'CTAG_G
Cuts at: 4894 4894
Size: 8039
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1313 1346 3030 3063 1313
Size: 33 1684 33 6289
Fragments arranged by size:
6289 1684 33 33
BamHI G'GATC_C
Cuts at: 5064 5064
Size: 8039
BanI G'GyrC_C
Cuts at: 868 886 1437 3367 5055 5469 5717 5752 868
Size: 18 551 1930 1688 414 248 35 3155
Fragments arranged by size:
3155 1930 1688 551 414 248 35 18
BanII G_rGCy'C
Cuts at: 115 130 142 997 1412 1829 5053 6083 115
Size: 15 12 855 415 417 3224 1030 2071
Fragments arranged by size:
3224 2071 1030 855 417 415 15 12
BbeI G_GCGC'C
Cuts at: 890 3371 5473 5721 890
Size: 2481 2102 248 3208
Fragments arranged by size:
3208 2481 2102 248
BbsI GAAGACnn'nnnn_
Cuts at: 764 2448 4186 764
Size: 1684 1738 4617
Fragments arranged by size:
4617 1738 1684
BciVI GTATCCnnnnn_n'
Cuts at: 459 2063 3370 4074 5937 6698 8039 459
Size: 1604 1307 704 1863 761 1341 459
Fragments arranged by size:
1863 1604 1341 1307 761 704 459
BclI T'GATC_A
Cuts at: 596 3557 5559 596
Size: 2961 2002 3076
Fragments arranged by size:
3076 2961 2002
BglI GCCn_nnn'nGGC
Cuts at: 1416 2155 1416
Size: 739 7300
BglII A'GATC_T
Cuts at: 1394 2681 2931 4930 5554 7591 1394
Size: 1287 250 1999 624 2037 1842
Fragments arranged by size:
2037 1999 1842 1287 624 250
BlpI GC'TnA_GC
Cuts at: 1348 3878 4712 5151 1348
Size: 2530 834 439 4236
Fragments arranged by size:
4236 2530 834 439
Bme1580I G_kGCm'C
Cuts at: 968 997 1412 4213 5664 5757 6813 968
Size: 29 415 2801 1451 93 1056 2194
Fragments arranged by size:
2801 2194 1451 1056 415 93 29
BmgBI CAC'GTC
Cuts at: 1075 4097 4158 1075
Size: 3022 61 4956
Fragments arranged by size:
4956 3022 61
BmrI ACTGGGnnnn_n'
Cuts at: 961 1602 1744 4216 5251 5332 5667 7811 961
Size: 641 142 2472 1035 81 335 2144 1189
Fragments arranged by size:
2472 2144 1189 1035 641 335 142 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2878 3222 6406 2878
Size: 344 3184 4511
Fragments arranged by size:
4511 3184 344
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 529 1322 1732 1855 1949 2361 2785 3365 529
Size: 793 410 123 94 412 424 580 5203
Fragments arranged by size:
5203 793 580 424 412 410 123 94
Bpu10I CC'TnA_GC
Cuts at: 3664 3664
Size: 8039
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 118 2604 5215 6094 6586 6884 118
Size: 2486 2611 879 492 298 1273
Fragments arranged by size:
2611 2486 1273 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 982 7743 982
Size: 6761 1278
BsaAI yAC'GTr
Cuts at: 666 3090 5392 666
Size: 2424 2302 3313
Fragments arranged by size:
3313 2424 2302
BsaBI GATnn'nnATC
Cuts at: 6 2815 3112 4860 5553 7590 6
Size: 2809 297 1748 693 2037 455
Fragments arranged by size:
2809 2037 1748 693 455 297
BsaHI Gr'CG_yC
Cuts at: 887 3368 5470 5718 6420 7916 887
Size: 2481 2102 248 702 1496 1010
Fragments arranged by size:
2481 2102 1496 1010 702 248
BsaWI w'CCGG_w
Cuts at: 5228 5749 6427 6701 6848 7259 5228
Size: 521 678 274 147 411 6008
Fragments arranged by size:
6008 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 676 706 2176 2206 676
Size: 30 1470 30 6509
Fragments arranged by size:
6509 1470 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 681 1729 1865 2650 3211 3349 3688 3715 681
Size: 1048 136 785 561 138 339 27 5005
Fragments arranged by size:
5005 1048 785 561 339 138 136 27
BseYI C'CCAG_C
Cuts at: 298 5464 6222 6799 7295 298
Size: 5166 758 577 496 1042
Fragments arranged by size:
5166 1042 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3733 3733
Size: 8039
BsiEI CG_ry'CG
Cuts at: 104 3603 5581 5627 6835 104
Size: 3499 1978 46 1208 1308
Fragments arranged by size:
3499 1978 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 115 142 830 2648 3155 3722 5053 5831 6021
Size: 27 688 1818 507 567 1331 778 190
Cuts at: 6021 6813 115
Size: 792 1341
Fragments arranged by size:
1818 1341 1331 792 778 688 567 507
190 27
BsmI GAATG_Cn'
Cuts at: 1605 3915 5035 1605
Size: 2310 1120 4609
Fragments arranged by size:
4609 2310 1120
BsmBI CGTCTCn'nnnn_
Cuts at: 3598 3598
Size: 8039
Bsp1286I G_dGCh'C
Cuts at: 115 130 142 830 968 997 1412 1829 2648
Size: 15 12 688 138 29 415 417 819
Cuts at: 2648 3155 3722 4213 5053 5664 5757 5831 6021
Size: 507 567 491 840 611 93 74 190
Cuts at: 6021 6083 6813 115
Size: 62 730 1341
Fragments arranged by size:
1341 840 819 730 688 611 567 507
491 417 415 190 138 93 74 62
29 15 12
BspEI T'CCGG_A
Cuts at: 7259 7259
Size: 8039
BspHI T'CATG_A
Cuts at: 2954 3155 3528 7215 2954
Size: 201 373 3687 3778
Fragments arranged by size:
3778 3687 373 201
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1034 2362 2413 2756 3440 5089 5303 5605 5986
Size: 1328 51 343 684 1649 214 302 381
Cuts at: 5986 1034
Size: 3087
Fragments arranged by size:
3087 1649 1328 684 381 343 302 214
51
BsrBI CCG'CTC
Cuts at: 50 6331 6385 7869 8034 50
Size: 6281 54 1484 165 55
Fragments arranged by size:
6281 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2393 4642 5951 2393
Size: 2249 1309 4481
Fragments arranged by size:
4481 2249 1309
BsrFI r'CCGG_y
Cuts at: 6037 6218 6427 6037
Size: 181 209 7649
Fragments arranged by size:
7649 209 181
BssSI C'ACGA_G
Cuts at: 970 979 1828 2062 2185 2935 6310 6668 970
Size: 9 849 234 123 750 3375 358 2341
Fragments arranged by size:
3375 2341 849 750 358 234 123 9
BstAPI GCAn_nnn'nTGC
Cuts at: 963 1617 3711 5416 963
Size: 654 2094 1705 3586
Fragments arranged by size:
3586 2094 1705 654
BstBI TT'CG_AA
Cuts at: 6400 6400
Size: 8039
BstXI CCAn_nnnn'nTGG
Cuts at: 1017 1050 2151 1017
Size: 33 1101 6905
Fragments arranged by size:
6905 1101 33
BstYI r'GATC_y
Cuts at: 1 626 1394 2681 2858 2931 4930 5064 5531
Size: 625 768 1287 177 73 1999 134 467
Cuts at: 5531 5554 5889 6135 7147 7233 7245 7591 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1999 1287 1012 768 625 467 449 346
335 246 177 134 86 73 23 12
Bsu36I CC'TnA_GG
Cuts at: 1141 1141
Size: 8039
BtgI C'CryG_G
Cuts at: 733 1484 2177 2369 3293 733
Size: 751 693 192 924 5479
Fragments arranged by size:
5479 924 751 693 192
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1630 5989 6170 6333 1630
Size: 4359 181 163 3336
Fragments arranged by size:
4359 3336 181 163
BtsI GCAGTG_nn'
Cuts at: 472 2087 2114 3712 4709 5282 472
Size: 1615 27 1598 997 573 3229
Fragments arranged by size:
3229 1615 1598 997 573 27
ClaI AT'CG_AT
Cuts at: 4044 4044
Size: 8039
DraI TTT'AAA
Cuts at: 4367 5038 4367
Size: 671 7368
DrdI GACnn_nn'nnGTC
Cuts at: 3538 5745 6603 3538
Size: 2207 858 4974
Fragments arranged by size:
4974 2207 858
EaeI y'GGCC_r
Cuts at: 252 1417 1487 2141 2253 2366 2791 2939 3003
Size: 1165 70 654 112 113 425 148 64
Cuts at: 3003 3600 5624 5798 6189 6216 7561 252
Size: 597 2024 174 391 27 1345 730
Fragments arranged by size:
2024 1345 1165 730 654 597 425 391
174 148 113 112 70 64 27
EagI C'GGCC_G
Cuts at: 3600 5624 3600
Size: 2024 6015
EarI CTCTTCn'nnn_
Cuts at: 305 1028 1500 2000 2126 2579 3664 4058 4142
Size: 723 472 500 126 453 1085 394 84
Cuts at: 4142 4268 6062 6272 305
Size: 126 1794 210 2072
Fragments arranged by size:
2072 1794 1085 723 500 472 453 394
210 126 126 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 431 1136 1781 6247 6557 6703 7849 431
Size: 705 645 4466 310 146 1146 621
Fragments arranged by size:
4466 1146 705 645 621 310 146
EcoICRI GAG'CTC
Cuts at: 113 140 5051 113
Size: 27 4911 3101
Fragments arranged by size:
4911 3101 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 529 1322 1732 1855 1949 2361 2491 2785 2893
Size: 793 410 123 94 412 130 294 108
Cuts at: 2893 3365 4292 4781 5864 6296 7043 7311 529
Size: 472 927 489 1083 432 747 268 1257
Fragments arranged by size:
1257 1083 927 793 747 489 472 432
412 410 294 268 130 123 108 94
EcoNI CCTnn'n_nnAGG
Cuts at: 598 3952 598
Size: 3354 4685
EcoO109I rG'GnC_Cy
Cuts at: 620 993 994 1409 2148 5178 620
Size: 373 1 415 739 3030 3481
Fragments arranged by size:
3481 3030 739 415 373 1
EcoRI G'AATT_C
Cuts at: 403 2689 5043 403
Size: 2286 2354 3399
Fragments arranged by size:
3399 2354 2286
EcoRV GAT'ATC
Cuts at: 3027 3508 4048 3027
Size: 481 540 7018
Fragments arranged by size:
7018 540 481
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2923 3851 6058 2923
Size: 928 2207 4904
Fragments arranged by size:
4904 2207 928
FspI TGC'GCA
Cuts at: 1100 5820 1100
Size: 4720 3319
HaeII r_GCGC'y
Cuts at: 890 3371 4025 5473 5721 6743 7722 890
Size: 2481 654 1448 248 1022 979 1207
Fragments arranged by size:
2481 1448 1207 1022 979 654 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 334 366 676 708 1075 1107 2203 2235 2524
Size: 32 310 32 367 32 1096 32 289
Cuts at: 2524 2556 2602 2634 3641 3673 3733 3765 4069
Size: 32 46 32 1007 32 60 32 304
Cuts at: 4069 4101 5881 5911 5913 5943 334
Size: 32 1780 30 2 30 2430
Fragments arranged by size:
2430 1780 1096 1007 367 310 304 289
60 46 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1647 3069 3864 4581 5078 1647
Size: 1422 795 717 497 4608
Fragments arranged by size:
4608 1422 795 717 497
HindIII A'AGCT_T
Cuts at: 4539 5012 4539
Size: 473 7566
HpaI GTT'AAC
Cuts at: 4581 4581
Size: 8039
KasI G'GCGC_C
Cuts at: 886 3367 5469 5717 886
Size: 2481 2102 248 3208
Fragments arranged by size:
3208 2481 2102 248
KpnI G_GTAC'C
Cuts at: 5059 5059
Size: 8039
MfeI C'AATT_G
Cuts at: 4618 4618
Size: 8039
MluI A'CGCG_T
Cuts at: 7472 7472
Size: 8039
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 747 827 3506 3791 4673 6710 6894 747
Size: 80 2679 285 882 2037 184 1892
Fragments arranged by size:
2679 2037 1892 882 285 184 80
MscI TGG'CCA
Cuts at: 254 1489 2143 2941 5800 254
Size: 1235 654 798 2859 2493
Fragments arranged by size:
2859 2493 1235 798 654
MslI CAynn'nnrTG
Cuts at: 1048 1291 2020 2773 2953 3586 6028 6155 1048
Size: 243 729 753 180 633 2442 127 2932
Fragments arranged by size:
2932 2442 753 729 633 243 180 127
MspA1I CmG'CkG
Cuts at: 504 1070 1206 1347 1862 3513 3599 3605 5150
Size: 566 136 141 515 1651 86 6 1545
Cuts at: 5150 5464 5824 6837 7082 504
Size: 314 360 1013 245 1461
Fragments arranged by size:
1651 1545 1461 1013 566 515 360 314
245 141 136 86 6
NaeI GCC'GGC
Cuts at: 6220 6220
Size: 8039
NarI GG'CG_CC
Cuts at: 887 3368 5470 5718 887
Size: 2481 2102 248 3208
Fragments arranged by size:
3208 2481 2102 248
NcoI C'CATG_G
Cuts at: 733 1484 2177 3293 733
Size: 751 693 1116 5479
Fragments arranged by size:
5479 1116 751 693
NdeI CA'TA_TG
Cuts at: 2529 2529
Size: 8039
NgoMIV G'CCGG_C
Cuts at: 6218 6218
Size: 8039
NotI GC'GGCC_GC
Cuts at: 3600 3600
Size: 8039
NspI r_CATG'y
Cuts at: 1686 3123 3240 4490 4942 5092 5592 6499 1686
Size: 1437 117 1250 452 150 500 907 3226
Fragments arranged by size:
3226 1437 1250 907 500 452 150 117
PasI CC'CwG_GG
Cuts at: 873 1425 873
Size: 552 7487
PciI A'CATG_T
Cuts at: 6495 6495
Size: 8039
PflMI CCAn_nnn'nTGG
Cuts at: 486 2103 2229 4297 486
Size: 1617 126 2068 4228
Fragments arranged by size:
4228 2068 1617 126
PfoI T'CCnGG_A
Cuts at: 2801 3050 3725 2801
Size: 249 675 7115
Fragments arranged by size:
7115 675 249
PmeI GTTT'AAAC
Cuts at: 5038 5038
Size: 8039
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7178 7210 7178
Size: 32 8007
PshAI GACnn'nnGTC
Cuts at: 4111 4111
Size: 8039
PsiI TTA'TAA
Cuts at: 1184 1219 1724 1184
Size: 35 505 7499
Fragments arranged by size:
7499 505 35
PspOMI G'GGCC_C
Cuts at: 993 1408 993
Size: 415 7624
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2974 3006 2974
Size: 32 8007
PstI C_TGCA'G
Cuts at: 935 2410 3610 3857 4704 5086 5771 935
Size: 1475 1200 247 847 382 685 3203
Fragments arranged by size:
3203 1475 1200 847 685 382 247
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8039
PvuII CAG'CTG
Cuts at: 504 1206 1347 3513 5464 5824 504
Size: 702 141 2166 1951 360 2719
Fragments arranged by size:
2719 2166 1951 702 360 141
RsrII CG'GwC_CG
Cuts at: 6234 6234
Size: 8039
SacI G_AGCT'C
Cuts at: 115 142 5053 115
Size: 27 4911 3101
Fragments arranged by size:
4911 3101 27
SalI G'TCGA_C
Cuts at: 1645 5076 1645
Size: 3431 4608
SapI GCTCTTCn'nnn_
Cuts at: 1028 3664 6062 6272 1028
Size: 2636 2398 210 2795
Fragments arranged by size:
2795 2636 2398 210
SbfI CC_TGCA'GG
Cuts at: 5086 5086
Size: 8039
ScaI AGT'ACT
Cuts at: 1108 2917 7556 1108
Size: 1809 4639 1591
Fragments arranged by size:
4639 1809 1591
SexAI A'CCwGG_T
Cuts at: 761 3485 761
Size: 2724 5315
SfcI C'TryA_G
Cuts at: 33 719 931 2406 2977 3606 3853 4032 4700
Size: 686 212 1475 571 629 247 179 668
Cuts at: 4700 5082 5767 6760 6951 33
Size: 382 685 993 191 1121
Fragments arranged by size:
1475 1121 993 686 685 668 629 571
382 247 212 191 179
SfiI GGCCn_nnn'nGGCC
Cuts at: 1416 2155 1416
Size: 739 7300
SfoI GGC'GCC
Cuts at: 888 3369 5471 5719 888
Size: 2481 2102 248 3208
Fragments arranged by size:
3208 2481 2102 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8039
SmaI CCC'GGG
Cuts at: 3967 4555 5061 3967
Size: 588 506 6945
Fragments arranged by size:
6945 588 506
SmlI C'TyrA_G
Cuts at: 133 2619 5194 6109 6601 6863 133
Size: 2486 2575 915 492 262 1309
Fragments arranged by size:
2575 2486 1309 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8039
SphI G_CATG'C
Cuts at: 5092 5592 5092
Size: 500 7539
StuI AGG'CCT
Cuts at: 542 593 1923 542
Size: 51 1330 6658
Fragments arranged by size:
6658 1330 51
StyI C'CwwG_G
Cuts at: 733 1484 1496 2144 2177 2555 3293 4894 5173
Size: 751 12 648 33 378 738 1601 279
Cuts at: 5173 5527 733
Size: 354 3245
Fragments arranged by size:
3245 1601 751 738 648 378 354 279
33 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 312 382 859 1497 1584 2440 5622 5755 6214
Size: 70 477 638 87 856 3182 133 459
Cuts at: 6214 6423 312
Size: 209 1928
Fragments arranged by size:
3182 1928 856 638 477 459 209 133
87 70
TatI w'GTAC_w
Cuts at: 1106 2915 3125 4595 7554 1106
Size: 1809 210 1470 2959 1591
Fragments arranged by size:
2959 1809 1591 1470 210
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2176 2208 4662 4694 7556 7588 2176
Size: 32 2454 32 2862 32 2627
Fragments arranged by size:
2862 2627 2454 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 798 1736 5836 798
Size: 938 4100 3001
Fragments arranged by size:
4100 3001 938
XbaI T'CTAG_A
Cuts at: 5070 5070
Size: 8039
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1707 2151 2511 3420 5534 1707
Size: 444 360 909 2114 4212
Fragments arranged by size:
4212 2114 909 444 360
XmaI C'CCGG_G
Cuts at: 3965 4553 5059 3965
Size: 588 506 6945
Fragments arranged by size:
6945 588 506
XmnI GAAnn'nnTTC
Cuts at: 1266 3658 4766 1266
Size: 2392 1108 4539
Fragments arranged by size:
4539 2392 1108
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AleI AloI AscI BbvCI BcgI BfrBI
BmtI BsiWI BsrGI BssHII BstEII BstZ17I CspCI DraIII
FseI FspAI NheI NruI NsiI PacI PmlI PpuMI
PspXI SacII SanDI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI