Restriction map for
pF1KE2586
(Circular) (Six-base) MAPSORT of: pF1KE2586.seq Check: 4679 from: 1 to: 5486
pF1KE2586 5486 bp
With 182 enzymes: SgfI *
August 7, 2015 15:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2016 2016
Size: 5486
AccI GT'mk_AC
Cuts at: 137 2524 137
Size: 2387 3099
Acc65I G'GTAC_C
Cuts at: 484 2502 484
Size: 2018 3468
AclI AA'CG_TT
Cuts at: 5326 5326
Size: 5486
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 1728 1971 3311 3743 4490 4758 213
Size: 1515 243 1340 432 747 268 941
Fragments arranged by size:
1515 1340 941 747 432 268 243
AflIII A'CryG_T
Cuts at: 620 2838 3942 4919 620
Size: 2218 1104 977 1187
Fragments arranged by size:
2218 1187 1104 977
AgeI A'CCGG_T
Cuts at: 3874 3874
Size: 5486
AleI CACnn'nnGTG
Cuts at: 1072 1969 1072
Size: 897 4589
AlwNI CAG_nnn'CTG
Cuts at: 753 1899 2420 2791 4358 753
Size: 1146 521 371 1567 1881
Fragments arranged by size:
1881 1567 1146 521 371
ApaI G_GGCC'C
Cuts at: 1677 1677
Size: 5486
ApaLI G'TGCA_C
Cuts at: 4256 4256
Size: 5486
ApoI r'AATT_y
Cuts at: 547 1256 2490 4839 5095 547
Size: 709 1234 2349 256 938
Fragments arranged by size:
2349 1234 938 709 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5486
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5486
AvaI C'yCGr_G
Cuts at: 2506 2506
Size: 5486
BamHI G'GATC_C
Cuts at: 352 2511 352
Size: 2159 3327
BanI G'GyrC_C
Cuts at: 477 484 895 1963 2502 2916 3164 3199 477
Size: 7 411 1068 539 414 248 35 2764
Fragments arranged by size:
2764 1068 539 414 411 248 35 7
BanII G_rGCy'C
Cuts at: 923 1416 1677 2054 2170 2367 2384 2500 3530
Size: 493 261 377 116 197 17 116 1030
Cuts at: 3530 923
Size: 2879
Fragments arranged by size:
2879 1030 493 377 261 197 116 116
17
BbeI G_GCGC'C
Cuts at: 2920 3168 2920
Size: 248 5238
BbsI GAAGACnn'nnnn_
Cuts at: 549 1581 2190 549
Size: 1032 609 3845
Fragments arranged by size:
3845 1032 609
BbvCI CC'TCA_GC
Cuts at: 240 1534 2036 240
Size: 1294 502 3690
Fragments arranged by size:
3690 1294 502
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 928 962 928
Size: 34 5452
BciVI GTATCCnnnnn_n'
Cuts at: 1397 3384 4145 5486 1397
Size: 1987 761 1341 1397
Fragments arranged by size:
1987 1397 1341 761
BclI T'GATC_A
Cuts at: 1355 3006 1355
Size: 1651 3835
BglI GCCn_nnn'nGGC
Cuts at: 604 2246 604
Size: 1642 3844
BglII A'GATC_T
Cuts at: 1739 3001 5038 1739
Size: 1262 2037 2187
Fragments arranged by size:
2187 2037 1262
BlpI GC'TnA_GC
Cuts at: 2164 2378 2598 2164
Size: 214 220 5052
Fragments arranged by size:
5052 220 214
Bme1580I G_kGCm'C
Cuts at: 1677 3111 3204 4260 1677
Size: 1434 93 1056 2903
Fragments arranged by size:
2903 1434 1056 93
BmgBI CAC'GTC
Cuts at: 1194 2283 2337 1194
Size: 1089 54 4343
Fragments arranged by size:
4343 1089 54
BmrI ACTGGGnnnn_n'
Cuts at: 196 2048 2430 2698 2779 3114 5258 196
Size: 1852 382 268 81 335 2144 424
Fragments arranged by size:
2144 1852 424 382 335 268 81
BmtI G_CTAG'C
Cuts at: 637 637
Size: 5486
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1877 3853 1877
Size: 1976 3510
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1328 2351 1328
Size: 1023 4463
Bpu10I CC'TnA_GC
Cuts at: 240 1534 2036 240
Size: 1294 502 3690
Fragments arranged by size:
3690 1294 502
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 1417 1429 1798 2662 3541 4033 4331 751
Size: 666 12 369 864 879 492 298 1906
Fragments arranged by size:
1906 879 864 666 492 369 298 12
BsaI GGTCTCn'nnnn_
Cuts at: 1092 1865 2458 5190 1092
Size: 773 593 2732 1388
Fragments arranged by size:
2732 1388 773 593
BsaAI yAC'GTr
Cuts at: 132 621 1706 2839 132
Size: 489 1085 1133 2779
Fragments arranged by size:
2779 1133 1085 489
BsaBI GATnn'nnATC
Cuts at: 6 1696 2143 3000 5037 6
Size: 1690 447 857 2037 455
Fragments arranged by size:
2037 1690 857 455 447
BsaHI Gr'CG_yC
Cuts at: 2249 2917 3165 3867 5363 2249
Size: 668 248 702 1496 2372
Fragments arranged by size:
2372 1496 702 668 248
BsaWI w'CCGG_w
Cuts at: 191 2675 3196 3874 4148 4295 4706 191
Size: 2484 521 678 274 147 411 971
Fragments arranged by size:
2484 971 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 414 1512 1869 1992 2416 414
Size: 1098 357 123 424 3484
Fragments arranged by size:
3484 1098 424 357 123
BseYI C'CCAG_C
Cuts at: 2290 2911 3669 4246 4742 2290
Size: 621 758 577 496 3034
Fragments arranged by size:
3034 758 621 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 601
Size: 5486
BsiEI CG_ry'CG
Cuts at: 104 737 3028 3074 4282 104
Size: 633 2291 46 1208 1308
Fragments arranged by size:
2291 1308 1208 633 46
BsiHKAI G_wGCw'C
Cuts at: 284 767 923 2170 2384 2500 3278 3468 4260
Size: 483 156 1247 214 116 778 190 792
Cuts at: 4260 284
Size: 1510
Fragments arranged by size:
1510 1247 792 778 483 214 190 156
116
BsmI GAATG_Cn'
Cuts at: 1923 2278 1923
Size: 355 5131
BsmBI CGTCTCn'nnnn_
Cuts at: 2327 2327
Size: 5486
Bsp1286I G_dGCh'C
Cuts at: 284 767 923 1416 1677 2054 2170 2367 2384
Size: 483 156 493 261 377 116 197 17
Cuts at: 2384 2500 3111 3204 3278 3468 3530 4260 284
Size: 116 611 93 74 190 62 730 1510
Fragments arranged by size:
1510 730 611 493 483 377 261 197
190 156 116 116 93 74 62 17
BspEI T'CCGG_A
Cuts at: 4706 4706
Size: 5486
BspHI T'CATG_A
Cuts at: 728 1868 4662 728
Size: 1140 2794 1552
Fragments arranged by size:
2794 1552 1140
BspMI ACCTGCnnnn'nnnn_
Cuts at: 338 2016 2536 2750 3052 3433 338
Size: 1678 520 214 302 381 2391
Fragments arranged by size:
2391 1678 520 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3778 3832 5316 5481 50
Size: 3728 54 1484 165 55
Fragments arranged by size:
3728 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 228 3398 228
Size: 3170 2316
BsrFI r'CCGG_y
Cuts at: 739 3484 3665 3874 739
Size: 2745 181 209 2351
Fragments arranged by size:
2745 2351 209 181
BsrGI T'GTAC_A
Cuts at: 2225 2225
Size: 5486
BssSI C'ACGA_G
Cuts at: 3757 4115 3757
Size: 358 5128
BstAPI GCAn_nnn'nTGC
Cuts at: 1899 2863 1899
Size: 964 4522
BstBI TT'CG_AA
Cuts at: 1201 1605 3847 1201
Size: 404 2242 2840
Fragments arranged by size:
2840 2242 404
BstYI r'GATC_y
Cuts at: 1 352 749 1376 1739 2511 2978 3001 3336
Size: 351 397 627 363 772 467 23 335
Cuts at: 3336 3582 4594 4680 4692 5038 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 772 627 467 449 397 363 351
346 335 246 86 23 12
BtgI C'CryG_G
Cuts at: 599 2241 599
Size: 1642 3844
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 849 1743 3436 3617 3780 849
Size: 894 1693 181 163 2555
Fragments arranged by size:
2555 1693 894 181 163
BtsI GCAGTG_nn'
Cuts at: 1562 2729 1562
Size: 1167 4319
DraI TTT'AAA
Cuts at: 1254 2485 1254
Size: 1231 4255
DrdI GACnn_nn'nnGTC
Cuts at: 3192 4050 3192
Size: 858 4628
EaeI y'GGCC_r
Cuts at: 392 692 734 1172 1325 2066 2156 3071 3245
Size: 300 42 438 153 741 90 915 174
Cuts at: 3245 3636 3663 5008 392
Size: 391 27 1345 870
Fragments arranged by size:
1345 915 870 741 438 391 300 174
153 90 42 27
EagI C'GGCC_G
Cuts at: 734 3071 734
Size: 2337 3149
EarI CTCTTCn'nnn_
Cuts at: 169 1474 3509 3719 169
Size: 1305 2035 210 1936
Fragments arranged by size:
2035 1936 1305 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3694 4004 4150 5296 3694
Size: 310 146 1146 3884
Fragments arranged by size:
3884 1146 310 146
EcoICRI GAG'CTC
Cuts at: 921 2168 2382 2498 921
Size: 1247 214 116 3909
Fragments arranged by size:
3909 1247 214 116
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 1328 1728 1971 2351 3311 3743 4490 4758
Size: 1115 400 243 380 960 432 747 268
Cuts at: 4758 213
Size: 941
Fragments arranged by size:
1115 960 941 747 432 400 380 268
243
EcoNI CCTnn'n_nnAGG
Cuts at: 1668 1668
Size: 5486
EcoO109I rG'GnC_Cy
Cuts at: 122 363 1673 2040 2151 2625 122
Size: 241 1310 367 111 474 2983
Fragments arranged by size:
2983 1310 474 367 241 111
EcoRI G'AATT_C
Cuts at: 2490 2490
Size: 5486
EcoRV GAT'ATC
Cuts at: 1683 1683
Size: 5486
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 792 966 2217 2301 3505 792
Size: 174 1251 84 1204 2773
Fragments arranged by size:
2773 1251 1204 174 84
FspI TGC'GCA
Cuts at: 3267 3267
Size: 5486
HaeII r_GCGC'y
Cuts at: 2920 3168 4190 5169 2920
Size: 248 1022 979 3237
Fragments arranged by size:
3237 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 773 805 1203 1235 1403 1435 1987 2019 3328
Size: 32 398 32 168 32 552 32 1309
Cuts at: 3328 3358 3360 3390 773
Size: 30 2 30 2869
Fragments arranged by size:
2869 1309 552 398 168 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2525 2525
Size: 5486
KasI G'GCGC_C
Cuts at: 2916 3164 2916
Size: 248 5238
KpnI G_GTAC'C
Cuts at: 488 2506 488
Size: 2018 3468
MluI A'CGCG_T
Cuts at: 4919 4919
Size: 5486
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1077 4157 4341 1077
Size: 3080 184 2222
Fragments arranged by size:
3080 2222 184
MscI TGG'CCA
Cuts at: 394 694 1174 1327 2068 2158 3247 394
Size: 300 480 153 741 90 1089 2633
Fragments arranged by size:
2633 1089 741 480 300 153 90
MslI CAynn'nnrTG
Cuts at: 508 658 1072 1648 1969 3475 3602 508
Size: 150 414 576 321 1506 127 2392
Fragments arranged by size:
2392 1506 576 414 321 150 127
MspA1I CmG'CkG
Cuts at: 347 531 893 2035 2597 2911 3271 4284 4529
Size: 184 362 1142 562 314 360 1013 245
Cuts at: 4529 347
Size: 1304
Fragments arranged by size:
1304 1142 1013 562 362 360 314 245
184
NaeI GCC'GGC
Cuts at: 741 3667 741
Size: 2926 2560
NarI GG'CG_CC
Cuts at: 2917 3165 2917
Size: 248 5238
NcoI C'CATG_G
Cuts at: 599 2241 599
Size: 1642 3844
NgoMIV G'CCGG_C
Cuts at: 739 3665 739
Size: 2926 2560
NheI G'CTAG_C
Cuts at: 633 633
Size: 5486
NspI r_CATG'y
Cuts at: 939 1035 2064 2454 2539 3039 3946 939
Size: 96 1029 390 85 500 907 2479
Fragments arranged by size:
2479 1029 907 500 390 96 85
PasI CC'CwG_GG
Cuts at: 808 808
Size: 5486
PciI A'CATG_T
Cuts at: 3942 3942
Size: 5486
PflMI CCAn_nnn'nTGG
Cuts at: 1632 1632
Size: 5486
PfoI T'CCnGG_A
Cuts at: 2105 2105
Size: 5486
PmeI GTTT'AAAC
Cuts at: 2485 2485
Size: 5486
PmlI CAC'GTG
Cuts at: 621 621
Size: 5486
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4625 4657 4625
Size: 32 5454
PpuMI rG'GwC_Cy
Cuts at: 363 363
Size: 5486
PspOMI G'GGCC_C
Cuts at: 1673 1673
Size: 5486
PstI C_TGCA'G
Cuts at: 408 530 749 1231 1895 1904 2533 3218 408
Size: 122 219 482 664 9 629 685 2676
Fragments arranged by size:
2676 685 664 629 482 219 122 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5486
PvuII CAG'CTG
Cuts at: 531 2911 3271 531
Size: 2380 360 2746
Fragments arranged by size:
2746 2380 360
RsrII CG'GwC_CG
Cuts at: 3681 3681
Size: 5486
SacI G_AGCT'C
Cuts at: 923 2170 2384 2500 923
Size: 1247 214 116 3909
Fragments arranged by size:
3909 1247 214 116
SalI G'TCGA_C
Cuts at: 2523 2523
Size: 5486
SapI GCTCTTCn'nnn_
Cuts at: 1474 3509 3719 1474
Size: 2035 210 3241
Fragments arranged by size:
3241 2035 210
SbfI CC_TGCA'GG
Cuts at: 1895 2533 1895
Size: 638 4848
ScaI AGT'ACT
Cuts at: 5003 5003
Size: 5486
SfcI C'TryA_G
Cuts at: 33 404 526 745 962 1227 1891 1900 2529
Size: 371 122 219 217 265 664 9 629
Cuts at: 2529 3214 4207 4398 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 664 629 371 265 219
217 191 122 9
SfoI GGC'GCC
Cuts at: 2918 3166 2918
Size: 248 5238
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5486
SmaI CCC'GGG
Cuts at: 2508 2508
Size: 5486
SmlI C'TyrA_G
Cuts at: 766 1432 1444 1813 2641 3556 4048 4310 766
Size: 666 12 369 828 915 492 262 1942
Fragments arranged by size:
1942 915 828 666 492 369 262 12
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5486
SphI G_CATG'C
Cuts at: 2539 3039 2539
Size: 500 4986
StyI C'CwwG_G
Cuts at: 599 2241 2358 2620 2974 599
Size: 1642 117 262 354 3111
Fragments arranged by size:
3111 1642 354 262 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1549 3069 3202 3661 3870 1549
Size: 1520 133 459 209 3165
Fragments arranged by size:
3165 1520 459 209 133
TatI w'GTAC_w
Cuts at: 770 2225 5001 770
Size: 1455 2776 1255
Fragments arranged by size:
2776 1455 1255
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5003 5035 5003
Size: 32 5454
Tth111I GACn'n_nGTC
Cuts at: 2305 3283 2305
Size: 978 4508
XbaI T'CTAG_A
Cuts at: 2268 2517 2268
Size: 249 5237
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 657 1368 1770 2061 2981 657
Size: 711 402 291 920 3162
Fragments arranged by size:
3162 920 711 402 291
XmaI C'CCGG_G
Cuts at: 2506 2506
Size: 5486
XmnI GAAnn'nnTTC
Cuts at: 2197 2197
Size: 5486
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BfrBI BsaXI BsiWI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII FseI FspAI HindIII HpaI
MfeI NdeI NotI NruI NsiI PacI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI