Restriction map for
pF1KE2602
(Circular) (Six-base) MAPSORT of: pF1KE2602.seq Check: 6067 from: 1 to: 5339
pF1KE2602 5339 bp
With 182 enzymes: SgfI *
January 19, 2016 12:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 190 190
Size: 5339
AccI GT'mk_AC
Cuts at: 1148 2377 1148
Size: 1229 4110
Acc65I G'GTAC_C
Cuts at: 2355 2355
Size: 5339
AclI AA'CG_TT
Cuts at: 5179 5179
Size: 5339
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 455 503 623 659 1105 1534 1778 3164 3596
Size: 48 120 36 446 429 244 1386 432
Cuts at: 3596 4343 4611 455
Size: 747 268 1183
Fragments arranged by size:
1386 1183 747 446 432 429 268 244
120 48 36
AflIII A'CryG_T
Cuts at: 2691 3795 4772 2691
Size: 1104 977 3258
Fragments arranged by size:
3258 1104 977
AgeI A'CCGG_T
Cuts at: 3727 3727
Size: 5339
AleI CACnn'nnGTG
Cuts at: 415 1940 415
Size: 1525 3814
AlwNI CAG_nnn'CTG
Cuts at: 737 802 1454 1876 2644 4211 737
Size: 65 652 422 768 1567 1865
Fragments arranged by size:
1865 1567 768 652 422 65
ApaLI G'TGCA_C
Cuts at: 4109 4109
Size: 5339
ApoI r'AATT_y
Cuts at: 2045 2343 4692 4948 2045
Size: 298 2349 256 2436
Fragments arranged by size:
2436 2349 298 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5339
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5339
AvaI C'yCGr_G
Cuts at: 2359 2359
Size: 5339
BamHI G'GATC_C
Cuts at: 377 2364 377
Size: 1987 3352
BanI G'GyrC_C
Cuts at: 562 1159 2355 2769 3017 3052 562
Size: 597 1196 414 248 35 2849
Fragments arranged by size:
2849 1196 597 414 248 35
BanII G_rGCy'C
Cuts at: 2353 3383 2353
Size: 1030 4309
BbeI G_GCGC'C
Cuts at: 2773 3021 2773
Size: 248 5091
BbsI GAAGACnn'nnnn_
Cuts at: 611 734 902 1367 611
Size: 123 168 465 4583
Fragments arranged by size:
4583 465 168 123
BbvCI CC'TCA_GC
Cuts at: 1395 1839 1395
Size: 444 4895
BciVI GTATCCnnnnn_n'
Cuts at: 3237 3998 5339 3237
Size: 761 1341 3237
Fragments arranged by size:
3237 1341 761
BclI T'GATC_A
Cuts at: 2060 2156 2859 2060
Size: 96 703 4540
Fragments arranged by size:
4540 703 96
BglII A'GATC_T
Cuts at: 2854 4891 2854
Size: 2037 3302
BlpI GC'TnA_GC
Cuts at: 157 2451 157
Size: 2294 3045
Bme1580I G_kGCm'C
Cuts at: 2964 3057 4113 2964
Size: 93 1056 4190
Fragments arranged by size:
4190 1056 93
BmgBI CAC'GTC
Cuts at: 639 639
Size: 5339
BmrI ACTGGGnnnn_n'
Cuts at: 438 1077 1825 1859 1934 2551 2632 2967 5111
Size: 639 748 34 75 617 81 335 2144
Cuts at: 5111 438
Size: 666
Fragments arranged by size:
2144 748 666 639 617 335 81 75
34
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1652 3706 1652
Size: 2054 3285
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 639 1005 1515 2252 639
Size: 366 510 737 3726
Fragments arranged by size:
3726 737 510 366
Bpu10I CC'TnA_GC
Cuts at: 1395 1839 1395
Size: 444 4895
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2515 3394 3886 4184 2515
Size: 879 492 298 3670
Fragments arranged by size:
3670 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 325 1231 1736 1913 5043 325
Size: 906 505 177 3130 621
Fragments arranged by size:
3130 906 621 505 177
BsaAI yAC'GTr
Cuts at: 213 2692 213
Size: 2479 2860
BsaBI GATnn'nnATC
Cuts at: 6 1971 2853 4890 6
Size: 1965 882 2037 455
Fragments arranged by size:
2037 1965 882 455
BsaHI Gr'CG_yC
Cuts at: 2770 3018 3720 5216 2770
Size: 248 702 1496 2893
Fragments arranged by size:
2893 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1107 2528 3049 3727 4001 4148 4559 1107
Size: 1421 521 678 274 147 411 1887
Fragments arranged by size:
1887 1421 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 373 403 373
Size: 30 5309
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 642 642
Size: 5339
BseYI C'CCAG_C
Cuts at: 616 932 2764 3522 4099 4595 616
Size: 316 1832 758 577 496 1360
Fragments arranged by size:
1832 1360 758 577 496 316
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 419 419
Size: 5339
BsiEI CG_ry'CG
Cuts at: 104 2881 2927 4135 104
Size: 2777 46 1208 1308
Fragments arranged by size:
2777 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 787 2353 3131 3321 4113 787
Size: 1566 778 190 792 2013
Fragments arranged by size:
2013 1566 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 787 2353 2964 3057 3131 3321 3383 4113 787
Size: 1566 611 93 74 190 62 730 2013
Fragments arranged by size:
2013 1566 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 1107 4559 1107
Size: 3452 1887
BspHI T'CATG_A
Cuts at: 1224 4515 1224
Size: 3291 2048
BspMI ACCTGCnnnn'nnnn_
Cuts at: 190 196 329 343 518 2312 2389 2603 2905
Size: 6 133 14 175 1794 77 214 302
Cuts at: 2905 3286 190
Size: 381 2243
Fragments arranged by size:
2243 1794 381 302 214 175 133 77
14 6
BsrBI CCG'CTC
Cuts at: 50 3631 3685 5169 5334 50
Size: 3581 54 1484 165 55
Fragments arranged by size:
3581 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2108 2175 3251 2108
Size: 67 1076 4196
Fragments arranged by size:
4196 1076 67
BsrFI r'CCGG_y
Cuts at: 3337 3518 3727 3337
Size: 181 209 4949
Fragments arranged by size:
4949 209 181
BsrGI T'GTAC_A
Cuts at: 419 493 708 419
Size: 74 215 5050
Fragments arranged by size:
5050 215 74
BssSI C'ACGA_G
Cuts at: 148 3610 3968 148
Size: 3462 358 1519
Fragments arranged by size:
3462 1519 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2716 2716
Size: 5339
BstBI TT'CG_AA
Cuts at: 3700 3700
Size: 5339
BstXI CCAn_nnnn'nTGG
Cuts at: 1942 1942
Size: 5339
BstYI r'GATC_y
Cuts at: 1 377 1966 2364 2831 2854 3189 3435 4447
Size: 376 1589 398 467 23 335 246 1012
Cuts at: 4447 4533 4545 4891 1
Size: 86 12 346 449
Fragments arranged by size:
1589 1012 467 449 398 376 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 1042 1487 107
Size: 935 445 3959
Fragments arranged by size:
3959 935 445
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3289 3470 3633 3289
Size: 181 163 4995
Fragments arranged by size:
4995 181 163
BtsI GCAGTG_nn'
Cuts at: 1163 2582 1163
Size: 1419 3920
ClaI AT'CG_AT
Cuts at: 173 173
Size: 5339
DraI TTT'AAA
Cuts at: 2338 2338
Size: 5339
DrdI GACnn_nn'nnGTC
Cuts at: 3045 3903 3045
Size: 858 4481
EaeI y'GGCC_r
Cuts at: 2924 3098 3489 3516 4861 2924
Size: 174 391 27 1345 3402
Fragments arranged by size:
3402 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2924 2924
Size: 5339
EarI CTCTTCn'nnn_
Cuts at: 1229 1734 3362 3572 1229
Size: 505 1628 210 2996
Fragments arranged by size:
2996 1628 505 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3547 3857 4003 5149 3547
Size: 310 146 1146 3737
Fragments arranged by size:
3737 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2351 2351
Size: 5339
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 455 503 623 639 659 1005 1105 1515 1534
Size: 48 120 16 20 346 100 410 19
Cuts at: 1534 1778 2252 3164 3596 4343 4611 455
Size: 244 474 912 432 747 268 1183
Fragments arranged by size:
1183 912 747 474 432 410 346 268
244 120 100 48 20 19 16
EcoNI CCTnn'n_nnAGG
Cuts at: 265 265
Size: 5339
EcoO109I rG'GnC_Cy
Cuts at: 1603 1820 1853 1949 2478 1603
Size: 217 33 96 529 4464
Fragments arranged by size:
4464 529 217 96 33
EcoRI G'AATT_C
Cuts at: 2343 2343
Size: 5339
EcoRV GAT'ATC
Cuts at: 1342 1342
Size: 5339
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1135 3358 1135
Size: 2223 3116
FspI TGC'GCA
Cuts at: 3120 3120
Size: 5339
HaeII r_GCGC'y
Cuts at: 2773 3021 4043 5022 2773
Size: 248 1022 979 3090
Fragments arranged by size:
3090 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 131 163 481 513 671 703 1003 1035 1513
Size: 32 318 32 158 32 300 32 478
Cuts at: 1513 1545 1590 1622 1876 1908 1969 2001 3181
Size: 32 45 32 254 32 61 32 1180
Cuts at: 3181 3211 3213 3243 131
Size: 30 2 30 2227
Fragments arranged by size:
2227 1180 478 318 300 254 158 61
45 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 552 2118 2378 552
Size: 1566 260 3513
Fragments arranged by size:
3513 1566 260
HpaI GTT'AAC
Cuts at: 552 552
Size: 5339
KasI G'GCGC_C
Cuts at: 2769 3017 2769
Size: 248 5091
KpnI G_GTAC'C
Cuts at: 2359 2359
Size: 5339
MluI A'CGCG_T
Cuts at: 4772 4772
Size: 5339
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 458 1711 1860 1913 4010 4194 458
Size: 1253 149 53 2097 184 1603
Fragments arranged by size:
2097 1603 1253 184 149 53
MscI TGG'CCA
Cuts at: 3100 3100
Size: 5339
MslI CAynn'nnrTG
Cuts at: 415 778 1135 1831 1940 1988 3328 3455 415
Size: 363 357 696 109 48 1340 127 2299
Fragments arranged by size:
2299 1340 696 363 357 127 109 48
MspA1I CmG'CkG
Cuts at: 156 256 647 1387 2287 2450 2764 3124 4137
Size: 100 391 740 900 163 314 360 1013
Cuts at: 4137 4382 156
Size: 245 1113
Fragments arranged by size:
1113 1013 900 740 391 360 314 245
163 100
NaeI GCC'GGC
Cuts at: 3520 3520
Size: 5339
NarI GG'CG_CC
Cuts at: 2770 3018 2770
Size: 248 5091
NcoI C'CATG_G
Cuts at: 107 1487 107
Size: 1380 3959
NgoMIV G'CCGG_C
Cuts at: 3518 3518
Size: 5339
NspI r_CATG'y
Cuts at: 364 2392 2892 3799 364
Size: 2028 500 907 1904
Fragments arranged by size:
2028 1904 907 500
PciI A'CATG_T
Cuts at: 3795 3795
Size: 5339
PfoI T'CCnGG_A
Cuts at: 1453 1453
Size: 5339
PmeI GTTT'AAAC
Cuts at: 2338 2338
Size: 5339
PmlI CAC'GTG
Cuts at: 213 213
Size: 5339
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4478 4510 4478
Size: 32 5307
PpuMI rG'GwC_Cy
Cuts at: 1603 1820 1853 1949 1603
Size: 217 33 96 4993
Fragments arranged by size:
4993 217 96 33
PshAI GACnn'nnGTC
Cuts at: 1532 1532
Size: 5339
PstI C_TGCA'G
Cuts at: 340 1392 1872 2309 2386 3071 340
Size: 1052 480 437 77 685 2608
Fragments arranged by size:
2608 1052 685 480 437 77
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5339
PvuII CAG'CTG
Cuts at: 1387 2287 2764 3124 1387
Size: 900 477 360 3602
Fragments arranged by size:
3602 900 477 360
RsrII CG'GwC_CG
Cuts at: 3534 3534
Size: 5339
SacI G_AGCT'C
Cuts at: 2353 2353
Size: 5339
SalI G'TCGA_C
Cuts at: 2376 2376
Size: 5339
SanDI GG'GwC_CC
Cuts at: 1949 1949
Size: 5339
SapI GCTCTTCn'nnn_
Cuts at: 3362 3572 3362
Size: 210 5129
SbfI CC_TGCA'GG
Cuts at: 340 2386 340
Size: 2046 3293
ScaI AGT'ACT
Cuts at: 4856 4856
Size: 5339
SfcI C'TryA_G
Cuts at: 33 231 336 794 1388 1868 2305 2382 3067
Size: 198 105 458 594 480 437 77 685
Cuts at: 3067 4060 4251 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 594 480 458 437 198
191 105 77
SfoI GGC'GCC
Cuts at: 2771 3019 2771
Size: 248 5091
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5339
SmaI CCC'GGG
Cuts at: 2361 2361
Size: 5339
SmlI C'TyrA_G
Cuts at: 2494 3409 3901 4163 2494
Size: 915 492 262 3670
Fragments arranged by size:
3670 915 492 262
SpeI A'CTAG_T
Cuts at: 67 787 67
Size: 720 4619
SphI G_CATG'C
Cuts at: 2392 2892 2392
Size: 500 4839
StyI C'CwwG_G
Cuts at: 107 1487 2084 2473 2827 107
Size: 1380 597 389 354 2619
Fragments arranged by size:
2619 1380 597 389 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1368 2922 3055 3514 3723 1368
Size: 1554 133 459 209 2984
Fragments arranged by size:
2984 1554 459 209 133
TatI w'GTAC_w
Cuts at: 419 493 708 1279 1540 4854 419
Size: 74 215 571 261 3314 904
Fragments arranged by size:
3314 904 571 261 215 74
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 373 405 4856 4888 373
Size: 32 4451 32 824
Fragments arranged by size:
4451 824 32 32
Tth111I GACn'n_nGTC
Cuts at: 1496 1851 3136 1496
Size: 355 1285 3699
Fragments arranged by size:
3699 1285 355
XbaI T'CTAG_A
Cuts at: 2370 2370
Size: 5339
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1960 2834 1960
Size: 874 4465
XmaI C'CCGG_G
Cuts at: 2359 2359
Size: 5339
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmtI BsiWI BsmI
BsmBI BssHII BstEII BstZ17I Bsu36I CspCI DraIII FseI
FspAI HindIII MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PsiI PspOMI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI