Restriction map for
pF1KE2603
(Circular) (Six-base) MAPSORT of: pF1KE2603.seq Check: 4247 from: 1 to: 6050
pF1KE2603 6050 bp
With 182 enzymes: SgfI *
January 19, 2016 12:01 ..
AccI GT'mk_AC
Cuts at: 3088 3088
Size: 6050
Acc65I G'GTAC_C
Cuts at: 3066 3066
Size: 6050
AclI AA'CG_TT
Cuts at: 5890 5890
Size: 6050
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 862 1079 1805 2404 2933 2951 3875 4307 5054
Size: 217 726 599 529 18 924 432 747
Cuts at: 5054 5322 862
Size: 268 1590
Fragments arranged by size:
1590 924 747 726 599 529 432 268
217 18
AflIII A'CryG_T
Cuts at: 1622 3402 4506 5483 1622
Size: 1780 1104 977 2189
Fragments arranged by size:
2189 1780 1104 977
AgeI A'CCGG_T
Cuts at: 4438 4438
Size: 6050
AlwNI CAG_nnn'CTG
Cuts at: 730 1966 2029 2929 3355 4922 730
Size: 1236 63 900 426 1567 1858
Fragments arranged by size:
1858 1567 1236 900 426 63
ApaLI G'TGCA_C
Cuts at: 2537 4820 2537
Size: 2283 3767
ApoI r'AATT_y
Cuts at: 412 3054 5403 5659 412
Size: 2642 2349 256 803
Fragments arranged by size:
2642 2349 803 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6050
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6050
AvaI C'yCGr_G
Cuts at: 3070 3070
Size: 6050
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1073 1106 1073
Size: 33 6017
BamHI G'GATC_C
Cuts at: 2803 3075 2803
Size: 272 5778
BanI G'GyrC_C
Cuts at: 858 2938 3066 3480 3728 3763 858
Size: 2080 128 414 248 35 3145
Fragments arranged by size:
3145 2080 414 248 128 35
BanII G_rGCy'C
Cuts at: 2065 3064 4094 2065
Size: 999 1030 4021
Fragments arranged by size:
4021 1030 999
BbeI G_GCGC'C
Cuts at: 2942 3484 3732 2942
Size: 542 248 5260
Fragments arranged by size:
5260 542 248
BbsI GAAGACnn'nnnn_
Cuts at: 850 2854 850
Size: 2004 4046
BbvCI CC'TCA_GC
Cuts at: 746 746
Size: 6050
BciVI GTATCCnnnnn_n'
Cuts at: 3948 4709 6050 3948
Size: 761 1341 3948
Fragments arranged by size:
3948 1341 761
BclI T'GATC_A
Cuts at: 3570 3570
Size: 6050
BfrBI ATG'CAT
Cuts at: 133 133
Size: 6050
BglI GCCn_nnn'nGGC
Cuts at: 2277 2966 2277
Size: 689 5361
BglII A'GATC_T
Cuts at: 1981 3565 5602 1981
Size: 1584 2037 2429
Fragments arranged by size:
2429 2037 1584
BlpI GC'TnA_GC
Cuts at: 2347 3162 2347
Size: 815 5235
Bme1580I G_kGCm'C
Cuts at: 2541 3675 3768 4824 2541
Size: 1134 93 1056 3767
Fragments arranged by size:
3767 1134 1056 93
BmgBI CAC'GTC
Cuts at: 1899 1899
Size: 6050
BmrI ACTGGGnnnn_n'
Cuts at: 1522 3262 3343 3678 5822 1522
Size: 1740 81 335 2144 1750
Fragments arranged by size:
2144 1750 1740 335 81
BmtI G_CTAG'C
Cuts at: 775 775
Size: 6050
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1196 1274 4417 1196
Size: 78 3143 2829
Fragments arranged by size:
3143 2829 78
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 558 1661 1703 1962 2456 2663 134
Size: 424 1103 42 259 494 207 3521
Fragments arranged by size:
3521 1103 494 424 259 207 42
Bpu10I CC'TnA_GC
Cuts at: 494 746 494
Size: 252 5798
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2181 3226 4105 4597 4895 2181
Size: 1045 879 492 298 3336
Fragments arranged by size:
3336 1045 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 407 2697 5754 407
Size: 2290 3057 703
Fragments arranged by size:
3057 2290 703
BsaAI yAC'GTr
Cuts at: 3403 3403
Size: 6050
BsaBI GATnn'nnATC
Cuts at: 6 3564 5601 6
Size: 3558 2037 455
Fragments arranged by size:
3558 2037 455
BsaHI Gr'CG_yC
Cuts at: 2939 3481 3729 4431 5927 2939
Size: 542 248 702 1496 3062
Fragments arranged by size:
3062 1496 702 542 248
BsaWI w'CCGG_w
Cuts at: 3239 3760 4438 4712 4859 5270 3239
Size: 521 678 274 147 411 4019
Fragments arranged by size:
4019 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 215 245 338 368 387 417 1659 1689 1758
Size: 30 93 30 19 30 1242 30 69
Cuts at: 1758 1788 2358 2388 2732 2762 2828 2858 215
Size: 30 570 30 344 30 66 30 3407
Fragments arranged by size:
3407 1242 570 344 93 69 66 30
30 30 30 30 30 30 30 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 734 2453 2685 2756 2864 2917 734
Size: 1719 232 71 108 53 3867
Fragments arranged by size:
3867 1719 232 108 71 53
BseYI C'CCAG_C
Cuts at: 1137 1323 1755 1971 2245 2326 2896 3475 4233
Size: 186 432 216 274 81 570 579 758
Cuts at: 4233 4810 5306 1137
Size: 577 496 1881
Fragments arranged by size:
1881 758 579 577 570 496 432 274
216 186 81
BsiEI CG_ry'CG
Cuts at: 104 3592 3638 4846 104
Size: 3488 46 1208 1308
Fragments arranged by size:
3488 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 310 1999 2179 2541 3064 3842 4032 4824 310
Size: 1689 180 362 523 778 190 792 1536
Fragments arranged by size:
1689 1536 792 778 523 362 190 180
Bsp1286I G_dGCh'C
Cuts at: 310 1999 2065 2179 2541 3064 3675 3768 3842
Size: 1689 66 114 362 523 611 93 74
Cuts at: 3842 4032 4094 4824 310
Size: 190 62 730 1536
Fragments arranged by size:
1689 1536 730 611 523 362 190 114
93 74 66 62
BspEI T'CCGG_A
Cuts at: 5270 5270
Size: 6050
BspHI T'CATG_A
Cuts at: 5226 5226
Size: 6050
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2124 2559 2838 3100 3314 3616 3997 2124
Size: 435 279 262 214 302 381 4177
Fragments arranged by size:
4177 435 381 302 279 262 214
BsrBI CCG'CTC
Cuts at: 50 2396 4342 4396 5880 6045 50
Size: 2346 1946 54 1484 165 55
Fragments arranged by size:
2346 1946 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 182 1913 3962 182
Size: 1731 2049 2270
Fragments arranged by size:
2270 2049 1731
BsrFI r'CCGG_y
Cuts at: 2521 2907 4048 4229 4438 2521
Size: 386 1141 181 209 4133
Fragments arranged by size:
4133 1141 386 209 181
BssSI C'ACGA_G
Cuts at: 4321 4679 4321
Size: 358 5692
BstAPI GCAn_nnn'nTGC
Cuts at: 2839 3427 2839
Size: 588 5462
BstBI TT'CG_AA
Cuts at: 4411 4411
Size: 6050
BstEII G'GTnAC_C
Cuts at: 2992 2992
Size: 6050
BstXI CCAn_nnnn'nTGG
Cuts at: 897 1107 1215 1293 1335 1581 897
Size: 210 108 78 42 246 5366
Fragments arranged by size:
5366 246 210 108 78 42
BstYI r'GATC_y
Cuts at: 1 1981 2803 3026 3075 3542 3565 3900 4146
Size: 1980 822 223 49 467 23 335 246
Cuts at: 4146 5158 5244 5256 5602 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1980 1012 822 467 449 346 335 246
223 86 49 23 12
Bsu36I CC'TnA_GG
Cuts at: 668 668
Size: 6050
BtgI C'CryG_G
Cuts at: 107 992 2411 107
Size: 885 1419 3746
Fragments arranged by size:
3746 1419 885
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4000 4181 4344 4000
Size: 181 163 5706
Fragments arranged by size:
5706 181 163
BtsI GCAGTG_nn'
Cuts at: 1459 2090 3293 1459
Size: 631 1203 4216
Fragments arranged by size:
4216 1203 631
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1767 1802 2718 2753 1767
Size: 35 916 35 5064
Fragments arranged by size:
5064 916 35 35
DraI TTT'AAA
Cuts at: 3049 3049
Size: 6050
DrdI GACnn_nn'nnGTC
Cuts at: 2142 3756 4614 2142
Size: 1614 858 3578
Fragments arranged by size:
3578 1614 858
EaeI y'GGCC_r
Cuts at: 463 971 1445 1571 2318 2357 2909 2967 3635
Size: 508 474 126 747 39 552 58 668
Cuts at: 3635 3809 4200 4227 5572 463
Size: 174 391 27 1345 941
Fragments arranged by size:
1345 941 747 668 552 508 474 391
174 126 58 39 27
EagI C'GGCC_G
Cuts at: 3635 3635
Size: 6050
EarI CTCTTCn'nnn_
Cuts at: 416 794 4073 4283 416
Size: 378 3279 210 2183
Fragments arranged by size:
3279 2183 378 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4258 4568 4714 5860 4258
Size: 310 146 1146 4448
Fragments arranged by size:
4448 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3062 3062
Size: 6050
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 558 862 1079 1661 1703 1805 1962 2404
Size: 424 304 217 582 42 102 157 442
Cuts at: 2404 2456 2663 2933 2951 3875 4307 5054 5322
Size: 52 207 270 18 924 432 747 268
Cuts at: 5322 134
Size: 862
Fragments arranged by size:
924 862 747 582 442 432 424 304
270 268 217 207 157 102 52 42
18
EcoNI CCTnn'n_nnAGG
Cuts at: 889 1015 1057 1741 1869 2438 2738 2834 889
Size: 126 42 684 128 569 300 96 4105
Fragments arranged by size:
4105 684 569 300 128 126 96 42
EcoO109I rG'GnC_Cy
Cuts at: 1752 2887 3189 1752
Size: 1135 302 4613
Fragments arranged by size:
4613 1135 302
EcoRI G'AATT_C
Cuts at: 412 3054 412
Size: 2642 3408
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 195 2790 4069 195
Size: 2595 1279 2176
Fragments arranged by size:
2595 2176 1279
FspI TGC'GCA
Cuts at: 3831 3831
Size: 6050
HaeII r_GCGC'y
Cuts at: 2929 2942 3484 3732 4754 5733 2929
Size: 13 542 248 1022 979 3246
Fragments arranged by size:
3246 1022 979 542 248 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 322 354 928 960 1162 1194 1240 1272 1366
Size: 32 574 32 202 32 46 32 94
Cuts at: 1366 1398 1659 1691 1731 1763 2732 2745 2764
Size: 32 261 32 40 32 969 13 19
Cuts at: 2764 2777 3892 3922 3924 3954 322
Size: 13 1115 30 2 30 2418
Fragments arranged by size:
2418 1115 969 574 261 202 94 46
40 32 32 32 32 32 32 32
30 30 19 13 13 2
HincII GTy'rAC
Cuts at: 3089 3089
Size: 6050
KasI G'GCGC_C
Cuts at: 2938 3480 3728 2938
Size: 542 248 5260
Fragments arranged by size:
5260 542 248
KpnI G_GTAC'C
Cuts at: 3070 3070
Size: 6050
MfeI C'AATT_G
Cuts at: 2528 2528
Size: 6050
MluI A'CGCG_T
Cuts at: 5483 5483
Size: 6050
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 213 430 513 4721 4905 213
Size: 217 83 4208 184 1358
Fragments arranged by size:
4208 1358 217 184 83
MscI TGG'CCA
Cuts at: 465 973 1447 2359 2969 3811 465
Size: 508 474 912 610 842 2704
Fragments arranged by size:
2704 912 842 610 508 474
MslI CAynn'nnrTG
Cuts at: 545 2266 4039 4166 545
Size: 1721 1773 127 2429
Fragments arranged by size:
2429 1773 1721 127
MspA1I CmG'CkG
Cuts at: 586 658 1837 2026 2155 2230 2476 3161 3475
Size: 72 1179 189 129 75 246 685 314
Cuts at: 3475 3835 4848 5093 586
Size: 360 1013 245 1543
Fragments arranged by size:
1543 1179 1013 685 360 314 246 245
189 129 75 72
NaeI GCC'GGC
Cuts at: 2523 2909 4231 2523
Size: 386 1322 4342
Fragments arranged by size:
4342 1322 386
NarI GG'CG_CC
Cuts at: 2939 3481 3729 2939
Size: 542 248 5260
Fragments arranged by size:
5260 542 248
NcoI C'CATG_G
Cuts at: 107 992 107
Size: 885 5165
NgoMIV G'CCGG_C
Cuts at: 2521 2907 4229 2521
Size: 386 1322 4342
Fragments arranged by size:
4342 1322 386
NheI G'CTAG_C
Cuts at: 771 771
Size: 6050
NsiI A_TGCA'T
Cuts at: 135 135
Size: 6050
NspI r_CATG'y
Cuts at: 984 1626 2638 3103 3603 4510 984
Size: 642 1012 465 500 907 2524
Fragments arranged by size:
2524 1012 907 642 500 465
PciI A'CATG_T
Cuts at: 1622 4506 1622
Size: 2884 3166
PflMI CCAn_nnn'nTGG
Cuts at: 3015 3015
Size: 6050
PfoI T'CCnGG_A
Cuts at: 257 3029 257
Size: 2772 3278
PmeI GTTT'AAAC
Cuts at: 3049 3049
Size: 6050
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5189 5221 5189
Size: 32 6018
PpuMI rG'GwC_Cy
Cuts at: 1752 2887 1752
Size: 1135 4915
PshAI GACnn'nnGTC
Cuts at: 119 2162 119
Size: 2043 4007
PstI C_TGCA'G
Cuts at: 2121 2235 2835 3097 3782 2121
Size: 114 600 262 685 4389
Fragments arranged by size:
4389 685 600 262 114
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6050
PvuII CAG'CTG
Cuts at: 586 658 1837 2026 2155 2476 3475 3835 586
Size: 72 1179 189 129 321 999 360 2801
Fragments arranged by size:
2801 1179 999 360 321 189 129 72
RsrII CG'GwC_CG
Cuts at: 4245 4245
Size: 6050
SacI G_AGCT'C
Cuts at: 3064 3064
Size: 6050
SalI G'TCGA_C
Cuts at: 3087 3087
Size: 6050
SanDI GG'GwC_CC
Cuts at: 2887 2887
Size: 6050
SapI GCTCTTCn'nnn_
Cuts at: 4073 4283 4073
Size: 210 5840
SbfI CC_TGCA'GG
Cuts at: 2835 3097 2835
Size: 262 5788
ScaI AGT'ACT
Cuts at: 5567 5567
Size: 6050
SfcI C'TryA_G
Cuts at: 33 395 2117 2231 2589 2831 3093 3778 4771
Size: 362 1722 114 358 242 262 685 993
Cuts at: 4771 4962 33
Size: 191 1121
Fragments arranged by size:
1722 1121 993 685 362 358 262 242
191 114
SfiI GGCCn_nnn'nGGCC
Cuts at: 2966 2966
Size: 6050
SfoI GGC'GCC
Cuts at: 2940 3482 3730 2940
Size: 542 248 5260
Fragments arranged by size:
5260 542 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6050
SmaI CCC'GGG
Cuts at: 3072 3072
Size: 6050
SmlI C'TyrA_G
Cuts at: 2160 3205 4120 4612 4874 2160
Size: 1045 915 492 262 3336
Fragments arranged by size:
3336 1045 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6050
SphI G_CATG'C
Cuts at: 3103 3603 3103
Size: 500 5550
SspI AAT'ATT
Cuts at: 599 2628 599
Size: 2029 4021
StuI AGG'CCT
Cuts at: 205 1875 205
Size: 1670 4380
StyI C'CwwG_G
Cuts at: 107 776 890 974 992 1142 1208 1286 1328
Size: 669 114 84 18 150 66 78 42
Cuts at: 1328 1490 1574 2399 2618 2795 3014 3184 3538
Size: 162 84 825 219 177 219 170 354
Cuts at: 3538 107
Size: 2619
Fragments arranged by size:
2619 825 669 354 219 219 177 170
162 150 114 84 84 78 66 42
18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 963 1239 1317 1860 2280 3633 3766 4225 4434
Size: 276 78 543 420 1353 133 459 209
Cuts at: 4434 963
Size: 2579
Fragments arranged by size:
2579 1353 543 459 420 276 209 133
78
TatI w'GTAC_w
Cuts at: 5565 5565
Size: 6050
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 694 726 2828 2860 5567 5599 694
Size: 32 2102 32 2707 32 1145
Fragments arranged by size:
2707 2102 1145 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3847 3847
Size: 6050
XbaI T'CTAG_A
Cuts at: 3081 3081
Size: 6050
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3545 3545
Size: 6050
XmaI C'CCGG_G
Cuts at: 3070 3070
Size: 6050
XmnI GAAnn'nnTTC
Cuts at: 1691 1691
Size: 6050
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NsiI NspI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BcgI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstZ17I ClaI DraIII EcoRV FseI FspAI
HindIII HpaI NdeI NotI NruI PacI PasI PmlI
PsiI PspOMI PspXI PsrI SacII SexAI SgrAI SnaBI
SrfI SwaI XhoI ZraI