(Circular) (Six-base) MAPSORT of: pF1KE2604.seq Check: 1939 from: 1 to: 3650 pF1KE2604 3650 bp With 182 enzymes: SgfI * January 19, 2016 12:01 .. AccI GT'mk_AC Cuts at: 688 688 Size: 3650 Acc65I G'GTAC_C Cuts at: 666 666 Size: 3650 AclI AA'CG_TT Cuts at: 3490 3490 Size: 3650 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1475 1907 2654 2922 1475 Size: 432 747 268 2203 Fragments arranged by size: 2203 747 432 268 AflIII A'CryG_T Cuts at: 1002 2106 3083 1002 Size: 1104 977 1569 Fragments arranged by size: 1569 1104 977 AgeI A'CCGG_T Cuts at: 2038 2038 Size: 3650 AleI CACnn'nnGTG Cuts at: 521 521 Size: 3650 AlwNI CAG_nnn'CTG Cuts at: 387 955 2522 387 Size: 568 1567 1515 Fragments arranged by size: 1567 1515 568 ApaLI G'TGCA_C Cuts at: 2420 2420 Size: 3650 ApoI r'AATT_y Cuts at: 394 654 3003 3259 394 Size: 260 2349 256 785 Fragments arranged by size: 2349 785 260 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3650 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3650 AvaI C'yCGr_G Cuts at: 670 670 Size: 3650 BamHI G'GATC_C Cuts at: 675 675 Size: 3650 BanI G'GyrC_C Cuts at: 666 1080 1328 1363 666 Size: 414 248 35 2953 Fragments arranged by size: 2953 414 248 35 BanII G_rGCy'C Cuts at: 664 1694 664 Size: 1030 2620 BbeI G_GCGC'C Cuts at: 1084 1332 1084 Size: 248 3402 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 90 Size: 34 3616 BciVI GTATCCnnnnn_n' Cuts at: 193 1548 2309 3650 193 Size: 1355 761 1341 193 Fragments arranged by size: 1355 1341 761 193 BclI T'GATC_A Cuts at: 1170 1170 Size: 3650 BglI GCCn_nnn'nGGC Cuts at: 160 160 Size: 3650 BglII A'GATC_T Cuts at: 1165 3202 1165 Size: 2037 1613 BlpI GC'TnA_GC Cuts at: 762 762 Size: 3650 Bme1580I G_kGCm'C Cuts at: 1275 1368 2424 1275 Size: 93 1056 2501 Fragments arranged by size: 2501 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 862 943 1278 3422 862 Size: 81 335 2144 1090 Fragments arranged by size: 2144 1090 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 527 2017 527 Size: 1490 2160 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 439 539 439 Size: 100 3550 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 826 1705 2197 2495 826 Size: 879 492 298 1981 Fragments arranged by size: 1981 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 415 3354 415 Size: 2939 711 BsaAI yAC'GTr Cuts at: 1003 1003 Size: 3650 BsaBI GATnn'nnATC Cuts at: 6 1164 3201 6 Size: 1158 2037 455 Fragments arranged by size: 2037 1158 455 BsaHI Gr'CG_yC Cuts at: 1081 1329 2031 3527 1081 Size: 248 702 1496 1204 Fragments arranged by size: 1496 1204 702 248 BsaWI w'CCGG_w Cuts at: 839 1360 2038 2312 2459 2870 839 Size: 521 678 274 147 411 1619 Fragments arranged by size: 1619 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 597 597 Size: 3650 BseYI C'CCAG_C Cuts at: 1075 1833 2410 2906 1075 Size: 758 577 496 1819 Fragments arranged by size: 1819 758 577 496 BsiEI CG_ry'CG Cuts at: 104 160 1192 1238 2446 104 Size: 56 1032 46 1208 1308 Fragments arranged by size: 1308 1208 1032 56 46 BsiHKAI G_wGCw'C Cuts at: 664 1442 1632 2424 664 Size: 778 190 792 1890 Fragments arranged by size: 1890 792 778 190 Bsp1286I G_dGCh'C Cuts at: 664 1275 1368 1442 1632 1694 2424 664 Size: 611 93 74 190 62 730 1890 Fragments arranged by size: 1890 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 2870 2870 Size: 3650 BspHI T'CATG_A Cuts at: 2826 2826 Size: 3650 BspMI ACCTGCnnnn'nnnn_ Cuts at: 700 914 1216 1597 700 Size: 214 302 381 2753 Fragments arranged by size: 2753 381 302 214 BsrBI CCG'CTC Cuts at: 50 1942 1996 3480 3645 50 Size: 1892 54 1484 165 55 Fragments arranged by size: 1892 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1562 1562 Size: 3650 BsrFI r'CCGG_y Cuts at: 159 1648 1829 2038 159 Size: 1489 181 209 1771 Fragments arranged by size: 1771 1489 209 181 BssSI C'ACGA_G Cuts at: 521 1921 2279 521 Size: 1400 358 1892 Fragments arranged by size: 1892 1400 358 BstAPI GCAn_nnn'nTGC Cuts at: 1027 1027 Size: 3650 BstBI TT'CG_AA Cuts at: 2011 2011 Size: 3650 BstXI CCAn_nnnn'nTGG Cuts at: 304 304 Size: 3650 BstYI r'GATC_y Cuts at: 1 675 1142 1165 1500 1746 2758 2844 2856 Size: 674 467 23 335 246 1012 86 12 Cuts at: 2856 3202 1 Size: 346 449 Fragments arranged by size: 1012 674 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 297 303 297 Size: 6 3644 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1600 1781 1944 1600 Size: 181 163 3306 Fragments arranged by size: 3306 181 163 BtsI GCAGTG_nn' Cuts at: 893 893 Size: 3650 DraI TTT'AAA Cuts at: 649 649 Size: 3650 DrdI GACnn_nn'nnGTC Cuts at: 1356 2214 1356 Size: 858 2792 EaeI y'GGCC_r Cuts at: 300 1235 1409 1800 1827 3172 300 Size: 935 174 391 27 1345 778 Fragments arranged by size: 1345 935 778 391 174 27 EagI C'GGCC_G Cuts at: 1235 1235 Size: 3650 EarI CTCTTCn'nnn_ Cuts at: 322 1673 1883 322 Size: 1351 210 2089 Fragments arranged by size: 2089 1351 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1858 2168 2314 3460 1858 Size: 310 146 1146 2048 Fragments arranged by size: 2048 1146 310 146 EcoICRI GAG'CTC Cuts at: 662 662 Size: 3650 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 439 539 1475 1907 2654 2922 439 Size: 100 936 432 747 268 1167 Fragments arranged by size: 1167 936 747 432 268 100 EcoNI CCTnn'n_nnAGG Cuts at: 501 501 Size: 3650 EcoO109I rG'GnC_Cy Cuts at: 789 789 Size: 3650 EcoRI G'AATT_C Cuts at: 654 654 Size: 3650 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 228 1669 228 Size: 1441 2209 FspI TGC'GCA Cuts at: 1431 1431 Size: 3650 HaeII r_GCGC'y Cuts at: 1084 1332 2354 3333 1084 Size: 248 1022 979 1401 Fragments arranged by size: 1401 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 361 393 1492 1522 1524 1554 361 Size: 32 1099 30 2 30 2457 Fragments arranged by size: 2457 1099 32 30 30 2 HincII GTy'rAC Cuts at: 689 689 Size: 3650 KasI G'GCGC_C Cuts at: 1080 1328 1080 Size: 248 3402 KpnI G_GTAC'C Cuts at: 670 670 Size: 3650 MfeI C'AATT_G Cuts at: 365 365 Size: 3650 MluI A'CGCG_T Cuts at: 3083 3083 Size: 3650 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 177 2321 2505 177 Size: 2144 184 1322 Fragments arranged by size: 2144 1322 184 MscI TGG'CCA Cuts at: 302 1411 302 Size: 1109 2541 MslI CAynn'nnrTG Cuts at: 302 521 1639 1766 302 Size: 219 1118 127 2186 Fragments arranged by size: 2186 1118 219 127 MspA1I CmG'CkG Cuts at: 550 761 1075 1435 2448 2693 550 Size: 211 314 360 1013 245 1507 Fragments arranged by size: 1507 1013 360 314 245 211 NaeI GCC'GGC Cuts at: 1831 1831 Size: 3650 NarI GG'CG_CC Cuts at: 1081 1329 1081 Size: 248 3402 NcoI C'CATG_G Cuts at: 297 303 297 Size: 6 3644 NgoMIV G'CCGG_C Cuts at: 1829 1829 Size: 3650 NspI r_CATG'y Cuts at: 703 1203 2110 703 Size: 500 907 2243 Fragments arranged by size: 2243 907 500 PciI A'CATG_T Cuts at: 2106 2106 Size: 3650 PfoI T'CCnGG_A Cuts at: 263 263 Size: 3650 PmeI GTTT'AAAC Cuts at: 649 649 Size: 3650 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2789 2821 2789 Size: 32 3618 PshAI GACnn'nnGTC Cuts at: 427 427 Size: 3650 PstI C_TGCA'G Cuts at: 534 697 1382 534 Size: 163 685 2802 Fragments arranged by size: 2802 685 163 PvuI CG_AT'CG Cuts at: 104 104 Size: 3650 PvuII CAG'CTG Cuts at: 1075 1435 1075 Size: 360 3290 RsrII CG'GwC_CG Cuts at: 1845 1845 Size: 3650 SacI G_AGCT'C Cuts at: 664 664 Size: 3650 SalI G'TCGA_C Cuts at: 687 687 Size: 3650 SapI GCTCTTCn'nnn_ Cuts at: 1673 1883 1673 Size: 210 3440 SbfI CC_TGCA'GG Cuts at: 697 697 Size: 3650 ScaI AGT'ACT Cuts at: 3167 3167 Size: 3650 SfcI C'TryA_G Cuts at: 33 530 693 1378 2371 2562 33 Size: 497 163 685 993 191 1121 Fragments arranged by size: 1121 993 685 497 191 163 SfoI GGC'GCC Cuts at: 1082 1330 1082 Size: 248 3402 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3650 SmaI CCC'GGG Cuts at: 672 672 Size: 3650 SmlI C'TyrA_G Cuts at: 805 1720 2212 2474 805 Size: 915 492 262 1981 Fragments arranged by size: 1981 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3650 SphI G_CATG'C Cuts at: 703 1203 703 Size: 500 3150 StyI C'CwwG_G Cuts at: 297 303 784 1138 297 Size: 6 481 354 2809 Fragments arranged by size: 2809 481 354 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 386 588 632 1233 1366 1825 2034 386 Size: 202 44 601 133 459 209 2002 Fragments arranged by size: 2002 601 459 209 202 133 44 TatI w'GTAC_w Cuts at: 216 3165 216 Size: 2949 701 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 245 277 3167 3199 245 Size: 32 2890 32 696 Fragments arranged by size: 2890 696 32 32 Tth111I GACn'n_nGTC Cuts at: 1447 1447 Size: 3650 XbaI T'CTAG_A Cuts at: 681 681 Size: 3650 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1145 1145 Size: 3650 XmaI C'CCGG_G Cuts at: 670 670 Size: 3650 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI PshAI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AlfI AloI ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmlI PpuMI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI