Restriction map for
pF1KE2610
(Circular) (Six-base) MAPSORT of: pF1KE2610.seq Check: 5509 from: 1 to: 3959
pF1KE2610 3959 bp
With 182 enzymes: SgfI *
February 29, 2016 12:19 ..
AccI GT'mk_AC
Cuts at: 997 997
Size: 3959
Acc65I G'GTAC_C
Cuts at: 975 975
Size: 3959
AclI AA'CG_TT
Cuts at: 3799 3799
Size: 3959
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 925 1784 2216 2963 3231 925
Size: 859 432 747 268 1653
Fragments arranged by size:
1653 859 747 432 268
AflIII A'CryG_T
Cuts at: 1311 2415 3392 1311
Size: 1104 977 1878
Fragments arranged by size:
1878 1104 977
AgeI A'CCGG_T
Cuts at: 2347 2347
Size: 3959
AlwNI CAG_nnn'CTG
Cuts at: 843 1264 2831 843
Size: 421 1567 1971
Fragments arranged by size:
1971 1567 421
ApaI G_GGCC'C
Cuts at: 345 767 345
Size: 422 3537
ApaLI G'TGCA_C
Cuts at: 2729 2729
Size: 3959
ApoI r'AATT_y
Cuts at: 963 3312 3568 963
Size: 2349 256 1354
Fragments arranged by size:
2349 1354 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3959
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3959
AvaI C'yCGr_G
Cuts at: 979 979
Size: 3959
BamHI G'GATC_C
Cuts at: 668 984 668
Size: 316 3643
BanI G'GyrC_C
Cuts at: 859 975 1389 1637 1672 859
Size: 116 414 248 35 3146
Fragments arranged by size:
3146 414 248 116 35
BanII G_rGCy'C
Cuts at: 238 345 767 780 902 973 2003 238
Size: 107 422 13 122 71 1030 2194
Fragments arranged by size:
2194 1030 422 122 107 71 13
BbeI G_GCGC'C
Cuts at: 1393 1641 1393
Size: 248 3711
BbsI GAAGACnn'nnnn_
Cuts at: 576 744 576
Size: 168 3791
BciVI GTATCCnnnnn_n'
Cuts at: 1857 2618 3959 1857
Size: 761 1341 1857
Fragments arranged by size:
1857 1341 761
BclI T'GATC_A
Cuts at: 1479 1479
Size: 3959
BglI GCCn_nnn'nGGC
Cuts at: 340 418 784 340
Size: 78 366 3515
Fragments arranged by size:
3515 366 78
BglII A'GATC_T
Cuts at: 1474 3511 1474
Size: 2037 1922
BlpI GC'TnA_GC
Cuts at: 1071 1071
Size: 3959
Bme1580I G_kGCm'C
Cuts at: 345 767 1584 1677 2733 345
Size: 422 817 93 1056 1571
Fragments arranged by size:
1571 1056 817 422 93
BmgBI CAC'GTC
Cuts at: 133 133
Size: 3959
BmrI ACTGGGnnnn_n'
Cuts at: 661 756 1171 1252 1587 3731 661
Size: 95 415 81 335 2144 889
Fragments arranged by size:
2144 889 415 335 95 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2326 2326
Size: 3959
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1135 2014 2506 2804 1135
Size: 879 492 298 2290
Fragments arranged by size:
2290 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 484 3663 484
Size: 3179 780
BsaAI yAC'GTr
Cuts at: 1312 1312
Size: 3959
BsaBI GATnn'nnATC
Cuts at: 6 283 637 1473 3510 6
Size: 277 354 836 2037 455
Fragments arranged by size:
2037 836 455 354 277
BsaHI Gr'CG_yC
Cuts at: 1390 1638 2340 3836 1390
Size: 248 702 1496 1513
Fragments arranged by size:
1513 1496 702 248
BsaWI w'CCGG_w
Cuts at: 512 1148 1669 2347 2621 2768 3179 512
Size: 636 521 678 274 147 411 1292
Fragments arranged by size:
1292 678 636 521 411 274 147
BseYI C'CCAG_C
Cuts at: 137 425 917 1384 2142 2719 3215 137
Size: 288 492 467 758 577 496 881
Fragments arranged by size:
881 758 577 496 492 467 288
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 177 337 177
Size: 160 3799
BsiEI CG_ry'CG
Cuts at: 104 1501 1547 2755 104
Size: 1397 46 1208 1308
Fragments arranged by size:
1397 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 578 973 1751 1941 2733 578
Size: 395 778 190 792 1804
Fragments arranged by size:
1804 792 778 395 190
Bsp1286I G_dGCh'C
Cuts at: 238 345 578 767 780 902 973 1584 1677
Size: 107 233 189 13 122 71 611 93
Cuts at: 1677 1751 1941 2003 2733 238
Size: 74 190 62 730 1464
Fragments arranged by size:
1464 730 611 233 190 189 122 107
93 74 71 62 13
BspEI T'CCGG_A
Cuts at: 512 3179 512
Size: 2667 1292
BspHI T'CATG_A
Cuts at: 3135 3135
Size: 3959
BspMI ACCTGCnnnn'nnnn_
Cuts at: 309 1009 1223 1525 1906 309
Size: 700 214 302 381 2362
Fragments arranged by size:
2362 700 381 302 214
BsrBI CCG'CTC
Cuts at: 50 704 2251 2305 3789 3954 50
Size: 654 1547 54 1484 165 55
Fragments arranged by size:
1547 1484 654 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1871 1871
Size: 3959
BsrFI r'CCGG_y
Cuts at: 1957 2138 2347 1957
Size: 181 209 3569
Fragments arranged by size:
3569 209 181
BssSI C'ACGA_G
Cuts at: 2230 2588 2230
Size: 358 3601
BstAPI GCAn_nnn'nTGC
Cuts at: 573 1336 573
Size: 763 3196
BstBI TT'CG_AA
Cuts at: 2320 2320
Size: 3959
BstEII G'GTnAC_C
Cuts at: 300 321 300
Size: 21 3938
BstXI CCAn_nnnn'nTGG
Cuts at: 150 664 679 831 150
Size: 514 15 152 3278
Fragments arranged by size:
3278 514 152 15
BstYI r'GATC_y
Cuts at: 1 638 668 984 1451 1474 1809 2055 3067
Size: 637 30 316 467 23 335 246 1012
Cuts at: 3067 3153 3165 3511 1
Size: 86 12 346 449
Fragments arranged by size:
1012 637 467 449 346 335 316 246
86 30 23 12
BtgI C'CryG_G
Cuts at: 107 206 308 440 935 107
Size: 99 102 132 495 3131
Fragments arranged by size:
3131 495 132 102 99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1909 2090 2253 1909
Size: 181 163 3615
Fragments arranged by size:
3615 181 163
BtsI GCAGTG_nn'
Cuts at: 122 551 1202 122
Size: 429 651 2879
Fragments arranged by size:
2879 651 429
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 615 650 615
Size: 35 3924
DraI TTT'AAA
Cuts at: 958 958
Size: 3959
DrdI GACnn_nn'nnGTC
Cuts at: 1665 2523 1665
Size: 858 3101
EaeI y'GGCC_r
Cuts at: 203 531 623 1544 1718 2109 2136 3481 203
Size: 328 92 921 174 391 27 1345 681
Fragments arranged by size:
1345 921 681 391 328 174 92 27
EagI C'GGCC_G
Cuts at: 1544 1544
Size: 3959
EarI CTCTTCn'nnn_
Cuts at: 1982 2192 1982
Size: 210 3749
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 910 2167 2477 2623 3769 910
Size: 1257 310 146 1146 1100
Fragments arranged by size:
1257 1146 1100 310 146
EcoICRI GAG'CTC
Cuts at: 971 971
Size: 3959
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 925 1784 2216 2963 3231 925
Size: 859 432 747 268 1653
Fragments arranged by size:
1653 859 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 907 907
Size: 3959
EcoO109I rG'GnC_Cy
Cuts at: 341 763 764 1098 341
Size: 422 1 334 3202
Fragments arranged by size:
3202 422 334 1
EcoRI G'AATT_C
Cuts at: 963 963
Size: 3959
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1978 1978
Size: 3959
FspI TGC'GCA
Cuts at: 1740 1740
Size: 3959
HaeII r_GCGC'y
Cuts at: 187 1393 1641 2663 3642 187
Size: 1206 248 1022 979 504
Fragments arranged by size:
1206 1022 979 504 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1801 1831 1833 1863 1801
Size: 30 2 30 3897
Fragments arranged by size:
3897 30 30 2
HincII GTy'rAC
Cuts at: 998 998
Size: 3959
KasI G'GCGC_C
Cuts at: 1389 1637 1389
Size: 248 3711
KpnI G_GTAC'C
Cuts at: 979 979
Size: 3959
MluI A'CGCG_T
Cuts at: 3392 3392
Size: 3959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2630 2814 2630
Size: 184 3775
MscI TGG'CCA
Cuts at: 205 533 1720 205
Size: 328 1187 2444
Fragments arranged by size:
2444 1187 328
MslI CAynn'nnrTG
Cuts at: 307 313 1948 2075 307
Size: 6 1635 127 2191
Fragments arranged by size:
2191 1635 127 6
MspA1I CmG'CkG
Cuts at: 338 429 570 840 1070 1384 1744 2757 3002
Size: 91 141 270 230 314 360 1013 245
Cuts at: 3002 338
Size: 1295
Fragments arranged by size:
1295 1013 360 314 270 245 230 141
91
NaeI GCC'GGC
Cuts at: 2140 2140
Size: 3959
NarI GG'CG_CC
Cuts at: 1390 1638 1390
Size: 248 3711
NcoI C'CATG_G
Cuts at: 107 308 107
Size: 201 3758
NgoMIV G'CCGG_C
Cuts at: 2138 2138
Size: 3959
NspI r_CATG'y
Cuts at: 150 1012 1512 2419 150
Size: 862 500 907 1690
Fragments arranged by size:
1690 907 862 500
PciI A'CATG_T
Cuts at: 2415 2415
Size: 3959
PflMI CCAn_nnn'nTGG
Cuts at: 432 540 432
Size: 108 3851
PmeI GTTT'AAAC
Cuts at: 958 958
Size: 3959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 508 540 3098 3130 508
Size: 32 2558 32 1337
Fragments arranged by size:
2558 1337 32 32
PshAI GACnn'nnGTC
Cuts at: 602 602
Size: 3959
PspOMI G'GGCC_C
Cuts at: 341 763 341
Size: 422 3537
PstI C_TGCA'G
Cuts at: 569 1006 1691 569
Size: 437 685 2837
Fragments arranged by size:
2837 685 437
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3959
PvuII CAG'CTG
Cuts at: 429 570 1384 1744 429
Size: 141 814 360 2644
Fragments arranged by size:
2644 814 360 141
RsrII CG'GwC_CG
Cuts at: 2154 2154
Size: 3959
SacI G_AGCT'C
Cuts at: 973 973
Size: 3959
SalI G'TCGA_C
Cuts at: 996 996
Size: 3959
SapI GCTCTTCn'nnn_
Cuts at: 1982 2192 1982
Size: 210 3749
SbfI CC_TGCA'GG
Cuts at: 1006 1006
Size: 3959
ScaI AGT'ACT
Cuts at: 3476 3476
Size: 3959
SexAI A'CCwGG_T
Cuts at: 296 296
Size: 3959
SfcI C'TryA_G
Cuts at: 33 274 565 1002 1687 2680 2871 33
Size: 241 291 437 685 993 191 1121
Fragments arranged by size:
1121 993 685 437 291 241 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 340 340
Size: 3959
SfoI GGC'GCC
Cuts at: 1391 1639 1391
Size: 248 3711
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3959
SmaI CCC'GGG
Cuts at: 981 981
Size: 3959
SmlI C'TyrA_G
Cuts at: 1114 2029 2521 2783 1114
Size: 915 492 262 2290
Fragments arranged by size:
2290 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3959
SphI G_CATG'C
Cuts at: 1012 1512 1012
Size: 500 3459
StyI C'CwwG_G
Cuts at: 107 308 495 1093 1447 107
Size: 201 187 598 354 2619
Fragments arranged by size:
2619 598 354 201 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 747 1542 1675 2134 2343 747
Size: 795 133 459 209 2363
Fragments arranged by size:
2363 795 459 209 133
TatI w'GTAC_w
Cuts at: 3474 3474
Size: 3959
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 246 278 3476 3508 246
Size: 32 3198 32 697
Fragments arranged by size:
3198 697 32 32
Tth111I GACn'n_nGTC
Cuts at: 1756 1756
Size: 3959
XbaI T'CTAG_A
Cuts at: 990 990
Size: 3959
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1454 114
Size: 1340 2619
XmaI C'CCGG_G
Cuts at: 979 979
Size: 3959
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PpiI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BmtI BpmI
Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII
BstZ17I Bsu36I ClaI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PsiI PspXI PsrI SacII
SanDI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI