(Circular) (Six-base) MAPSORT of: pF1KE2610.seq Check: 5509 from: 1 to: 3959 pF1KE2610 3959 bp With 182 enzymes: SgfI * February 29, 2016 12:19 .. AccI GT'mk_AC Cuts at: 997 997 Size: 3959 Acc65I G'GTAC_C Cuts at: 975 975 Size: 3959 AclI AA'CG_TT Cuts at: 3799 3799 Size: 3959 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 925 1784 2216 2963 3231 925 Size: 859 432 747 268 1653 Fragments arranged by size: 1653 859 747 432 268 AflIII A'CryG_T Cuts at: 1311 2415 3392 1311 Size: 1104 977 1878 Fragments arranged by size: 1878 1104 977 AgeI A'CCGG_T Cuts at: 2347 2347 Size: 3959 AlwNI CAG_nnn'CTG Cuts at: 843 1264 2831 843 Size: 421 1567 1971 Fragments arranged by size: 1971 1567 421 ApaI G_GGCC'C Cuts at: 345 767 345 Size: 422 3537 ApaLI G'TGCA_C Cuts at: 2729 2729 Size: 3959 ApoI r'AATT_y Cuts at: 963 3312 3568 963 Size: 2349 256 1354 Fragments arranged by size: 2349 1354 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3959 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3959 AvaI C'yCGr_G Cuts at: 979 979 Size: 3959 BamHI G'GATC_C Cuts at: 668 984 668 Size: 316 3643 BanI G'GyrC_C Cuts at: 859 975 1389 1637 1672 859 Size: 116 414 248 35 3146 Fragments arranged by size: 3146 414 248 116 35 BanII G_rGCy'C Cuts at: 238 345 767 780 902 973 2003 238 Size: 107 422 13 122 71 1030 2194 Fragments arranged by size: 2194 1030 422 122 107 71 13 BbeI G_GCGC'C Cuts at: 1393 1641 1393 Size: 248 3711 BbsI GAAGACnn'nnnn_ Cuts at: 576 744 576 Size: 168 3791 BciVI GTATCCnnnnn_n' Cuts at: 1857 2618 3959 1857 Size: 761 1341 1857 Fragments arranged by size: 1857 1341 761 BclI T'GATC_A Cuts at: 1479 1479 Size: 3959 BglI GCCn_nnn'nGGC Cuts at: 340 418 784 340 Size: 78 366 3515 Fragments arranged by size: 3515 366 78 BglII A'GATC_T Cuts at: 1474 3511 1474 Size: 2037 1922 BlpI GC'TnA_GC Cuts at: 1071 1071 Size: 3959 Bme1580I G_kGCm'C Cuts at: 345 767 1584 1677 2733 345 Size: 422 817 93 1056 1571 Fragments arranged by size: 1571 1056 817 422 93 BmgBI CAC'GTC Cuts at: 133 133 Size: 3959 BmrI ACTGGGnnnn_n' Cuts at: 661 756 1171 1252 1587 3731 661 Size: 95 415 81 335 2144 889 Fragments arranged by size: 2144 889 415 335 95 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2326 2326 Size: 3959 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1135 2014 2506 2804 1135 Size: 879 492 298 2290 Fragments arranged by size: 2290 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 484 3663 484 Size: 3179 780 BsaAI yAC'GTr Cuts at: 1312 1312 Size: 3959 BsaBI GATnn'nnATC Cuts at: 6 283 637 1473 3510 6 Size: 277 354 836 2037 455 Fragments arranged by size: 2037 836 455 354 277 BsaHI Gr'CG_yC Cuts at: 1390 1638 2340 3836 1390 Size: 248 702 1496 1513 Fragments arranged by size: 1513 1496 702 248 BsaWI w'CCGG_w Cuts at: 512 1148 1669 2347 2621 2768 3179 512 Size: 636 521 678 274 147 411 1292 Fragments arranged by size: 1292 678 636 521 411 274 147 BseYI C'CCAG_C Cuts at: 137 425 917 1384 2142 2719 3215 137 Size: 288 492 467 758 577 496 881 Fragments arranged by size: 881 758 577 496 492 467 288 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 177 337 177 Size: 160 3799 BsiEI CG_ry'CG Cuts at: 104 1501 1547 2755 104 Size: 1397 46 1208 1308 Fragments arranged by size: 1397 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 578 973 1751 1941 2733 578 Size: 395 778 190 792 1804 Fragments arranged by size: 1804 792 778 395 190 Bsp1286I G_dGCh'C Cuts at: 238 345 578 767 780 902 973 1584 1677 Size: 107 233 189 13 122 71 611 93 Cuts at: 1677 1751 1941 2003 2733 238 Size: 74 190 62 730 1464 Fragments arranged by size: 1464 730 611 233 190 189 122 107 93 74 71 62 13 BspEI T'CCGG_A Cuts at: 512 3179 512 Size: 2667 1292 BspHI T'CATG_A Cuts at: 3135 3135 Size: 3959 BspMI ACCTGCnnnn'nnnn_ Cuts at: 309 1009 1223 1525 1906 309 Size: 700 214 302 381 2362 Fragments arranged by size: 2362 700 381 302 214 BsrBI CCG'CTC Cuts at: 50 704 2251 2305 3789 3954 50 Size: 654 1547 54 1484 165 55 Fragments arranged by size: 1547 1484 654 165 55 54 BsrDI GCAATG_nn' Cuts at: 1871 1871 Size: 3959 BsrFI r'CCGG_y Cuts at: 1957 2138 2347 1957 Size: 181 209 3569 Fragments arranged by size: 3569 209 181 BssSI C'ACGA_G Cuts at: 2230 2588 2230 Size: 358 3601 BstAPI GCAn_nnn'nTGC Cuts at: 573 1336 573 Size: 763 3196 BstBI TT'CG_AA Cuts at: 2320 2320 Size: 3959 BstEII G'GTnAC_C Cuts at: 300 321 300 Size: 21 3938 BstXI CCAn_nnnn'nTGG Cuts at: 150 664 679 831 150 Size: 514 15 152 3278 Fragments arranged by size: 3278 514 152 15 BstYI r'GATC_y Cuts at: 1 638 668 984 1451 1474 1809 2055 3067 Size: 637 30 316 467 23 335 246 1012 Cuts at: 3067 3153 3165 3511 1 Size: 86 12 346 449 Fragments arranged by size: 1012 637 467 449 346 335 316 246 86 30 23 12 BtgI C'CryG_G Cuts at: 107 206 308 440 935 107 Size: 99 102 132 495 3131 Fragments arranged by size: 3131 495 132 102 99 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1909 2090 2253 1909 Size: 181 163 3615 Fragments arranged by size: 3615 181 163 BtsI GCAGTG_nn' Cuts at: 122 551 1202 122 Size: 429 651 2879 Fragments arranged by size: 2879 651 429 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 615 650 615 Size: 35 3924 DraI TTT'AAA Cuts at: 958 958 Size: 3959 DrdI GACnn_nn'nnGTC Cuts at: 1665 2523 1665 Size: 858 3101 EaeI y'GGCC_r Cuts at: 203 531 623 1544 1718 2109 2136 3481 203 Size: 328 92 921 174 391 27 1345 681 Fragments arranged by size: 1345 921 681 391 328 174 92 27 EagI C'GGCC_G Cuts at: 1544 1544 Size: 3959 EarI CTCTTCn'nnn_ Cuts at: 1982 2192 1982 Size: 210 3749 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 910 2167 2477 2623 3769 910 Size: 1257 310 146 1146 1100 Fragments arranged by size: 1257 1146 1100 310 146 EcoICRI GAG'CTC Cuts at: 971 971 Size: 3959 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 925 1784 2216 2963 3231 925 Size: 859 432 747 268 1653 Fragments arranged by size: 1653 859 747 432 268 EcoNI CCTnn'n_nnAGG Cuts at: 907 907 Size: 3959 EcoO109I rG'GnC_Cy Cuts at: 341 763 764 1098 341 Size: 422 1 334 3202 Fragments arranged by size: 3202 422 334 1 EcoRI G'AATT_C Cuts at: 963 963 Size: 3959 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1978 1978 Size: 3959 FspI TGC'GCA Cuts at: 1740 1740 Size: 3959 HaeII r_GCGC'y Cuts at: 187 1393 1641 2663 3642 187 Size: 1206 248 1022 979 504 Fragments arranged by size: 1206 1022 979 504 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1801 1831 1833 1863 1801 Size: 30 2 30 3897 Fragments arranged by size: 3897 30 30 2 HincII GTy'rAC Cuts at: 998 998 Size: 3959 KasI G'GCGC_C Cuts at: 1389 1637 1389 Size: 248 3711 KpnI G_GTAC'C Cuts at: 979 979 Size: 3959 MluI A'CGCG_T Cuts at: 3392 3392 Size: 3959 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2630 2814 2630 Size: 184 3775 MscI TGG'CCA Cuts at: 205 533 1720 205 Size: 328 1187 2444 Fragments arranged by size: 2444 1187 328 MslI CAynn'nnrTG Cuts at: 307 313 1948 2075 307 Size: 6 1635 127 2191 Fragments arranged by size: 2191 1635 127 6 MspA1I CmG'CkG Cuts at: 338 429 570 840 1070 1384 1744 2757 3002 Size: 91 141 270 230 314 360 1013 245 Cuts at: 3002 338 Size: 1295 Fragments arranged by size: 1295 1013 360 314 270 245 230 141 91 NaeI GCC'GGC Cuts at: 2140 2140 Size: 3959 NarI GG'CG_CC Cuts at: 1390 1638 1390 Size: 248 3711 NcoI C'CATG_G Cuts at: 107 308 107 Size: 201 3758 NgoMIV G'CCGG_C Cuts at: 2138 2138 Size: 3959 NspI r_CATG'y Cuts at: 150 1012 1512 2419 150 Size: 862 500 907 1690 Fragments arranged by size: 1690 907 862 500 PciI A'CATG_T Cuts at: 2415 2415 Size: 3959 PflMI CCAn_nnn'nTGG Cuts at: 432 540 432 Size: 108 3851 PmeI GTTT'AAAC Cuts at: 958 958 Size: 3959 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 508 540 3098 3130 508 Size: 32 2558 32 1337 Fragments arranged by size: 2558 1337 32 32 PshAI GACnn'nnGTC Cuts at: 602 602 Size: 3959 PspOMI G'GGCC_C Cuts at: 341 763 341 Size: 422 3537 PstI C_TGCA'G Cuts at: 569 1006 1691 569 Size: 437 685 2837 Fragments arranged by size: 2837 685 437 PvuI CG_AT'CG Cuts at: 104 104 Size: 3959 PvuII CAG'CTG Cuts at: 429 570 1384 1744 429 Size: 141 814 360 2644 Fragments arranged by size: 2644 814 360 141 RsrII CG'GwC_CG Cuts at: 2154 2154 Size: 3959 SacI G_AGCT'C Cuts at: 973 973 Size: 3959 SalI G'TCGA_C Cuts at: 996 996 Size: 3959 SapI GCTCTTCn'nnn_ Cuts at: 1982 2192 1982 Size: 210 3749 SbfI CC_TGCA'GG Cuts at: 1006 1006 Size: 3959 ScaI AGT'ACT Cuts at: 3476 3476 Size: 3959 SexAI A'CCwGG_T Cuts at: 296 296 Size: 3959 SfcI C'TryA_G Cuts at: 33 274 565 1002 1687 2680 2871 33 Size: 241 291 437 685 993 191 1121 Fragments arranged by size: 1121 993 685 437 291 241 191 SfiI GGCCn_nnn'nGGCC Cuts at: 340 340 Size: 3959 SfoI GGC'GCC Cuts at: 1391 1639 1391 Size: 248 3711 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3959 SmaI CCC'GGG Cuts at: 981 981 Size: 3959 SmlI C'TyrA_G Cuts at: 1114 2029 2521 2783 1114 Size: 915 492 262 2290 Fragments arranged by size: 2290 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3959 SphI G_CATG'C Cuts at: 1012 1512 1012 Size: 500 3459 StyI C'CwwG_G Cuts at: 107 308 495 1093 1447 107 Size: 201 187 598 354 2619 Fragments arranged by size: 2619 598 354 201 187 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 747 1542 1675 2134 2343 747 Size: 795 133 459 209 2363 Fragments arranged by size: 2363 795 459 209 133 TatI w'GTAC_w Cuts at: 3474 3474 Size: 3959 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 246 278 3476 3508 246 Size: 32 3198 32 697 Fragments arranged by size: 3198 697 32 32 Tth111I GACn'n_nGTC Cuts at: 1756 1756 Size: 3959 XbaI T'CTAG_A Cuts at: 990 990 Size: 3959 XcmI CCAnnnn_n'nnnnTGG Cuts at: 114 1454 114 Size: 1340 2619 XmaI C'CCGG_G Cuts at: 979 979 Size: 3959 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI AscI AvrII BaeI BbvCI BcgI BfrBI BmtI BpmI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PfoI PmlI PpuMI PsiI PspXI PsrI SacII SanDI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI