Restriction map for
pF1KE2615
(Circular) (Six-base) MAPSORT of: pF1KE2615.seq Check: 7423 from: 1 to: 4175
pF1KE2615 4175 bp
With 182 enzymes: SgfI *
April 1, 2016 14:38 ..
AccI GT'mk_AC
Cuts at: 1117 1213 1117
Size: 96 4079
Acc65I G'GTAC_C
Cuts at: 1191 1191
Size: 4175
AclI AA'CG_TT
Cuts at: 4015 4015
Size: 4175
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 176 964 1117 2000 2432 3179 3447 176
Size: 788 153 883 432 747 268 904
Fragments arranged by size:
904 883 788 747 432 268 153
AfeI AGC'GCT
Cuts at: 532 1000 532
Size: 468 3707
AflIII A'CryG_T
Cuts at: 698 1527 2631 3608 698
Size: 829 1104 977 1265
Fragments arranged by size:
1265 1104 977 829
AgeI A'CCGG_T
Cuts at: 2563 2563
Size: 4175
AlwNI CAG_nnn'CTG
Cuts at: 196 713 1480 3047 196
Size: 517 767 1567 1324
Fragments arranged by size:
1567 1324 767 517
ApaLI G'TGCA_C
Cuts at: 2945 2945
Size: 4175
ApoI r'AATT_y
Cuts at: 588 1179 3528 3784 588
Size: 591 2349 256 979
Fragments arranged by size:
2349 979 591 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4175
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4175
AvaI C'yCGr_G
Cuts at: 1195 1195
Size: 4175
BamHI G'GATC_C
Cuts at: 1200 1200
Size: 4175
BanI G'GyrC_C
Cuts at: 451 642 1191 1605 1853 1888 451
Size: 191 549 414 248 35 2738
Fragments arranged by size:
2738 549 414 248 191 35
BanII G_rGCy'C
Cuts at: 1083 1189 2219 1083
Size: 106 1030 3039
Fragments arranged by size:
3039 1030 106
BbeI G_GCGC'C
Cuts at: 1609 1857 1609
Size: 248 3927
BbsI GAAGACnn'nnnn_
Cuts at: 164 770 164
Size: 606 3569
BbvCI CC'TCA_GC
Cuts at: 563 563
Size: 4175
BciVI GTATCCnnnnn_n'
Cuts at: 2073 2834 4175 2073
Size: 761 1341 2073
Fragments arranged by size:
2073 1341 761
BclI T'GATC_A
Cuts at: 1695 1695
Size: 4175
BglII A'GATC_T
Cuts at: 1690 3727 1690
Size: 2037 2138
BlpI GC'TnA_GC
Cuts at: 1287 1287
Size: 4175
Bme1580I G_kGCm'C
Cuts at: 647 1800 1893 2949 647
Size: 1153 93 1056 1873
Fragments arranged by size:
1873 1153 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 793 1387 1468 1803 3947 793
Size: 594 81 335 2144 1021
Fragments arranged by size:
2144 1021 594 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 552 684 1097 2542 552
Size: 132 413 1445 2185
Fragments arranged by size:
2185 1445 413 132
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 753 1071 753
Size: 318 3857
Bpu10I CC'TnA_GC
Cuts at: 563 563
Size: 4175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1024 1351 2230 2722 3020 1024
Size: 327 879 492 298 2179
Fragments arranged by size:
2179 879 492 327 298
BsaI GGTCTCn'nnnn_
Cuts at: 824 3879 824
Size: 3055 1120
BsaAI yAC'GTr
Cuts at: 365 1528 365
Size: 1163 3012
BsaBI GATnn'nnATC
Cuts at: 6 1689 3726 6
Size: 1683 2037 455
Fragments arranged by size:
2037 1683 455
BsaHI Gr'CG_yC
Cuts at: 1606 1854 2556 4052 1606
Size: 248 702 1496 1729
Fragments arranged by size:
1729 1496 702 248
BsaWI w'CCGG_w
Cuts at: 731 1364 1885 2563 2837 2984 3395 731
Size: 633 521 678 274 147 411 1511
Fragments arranged by size:
1511 678 633 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 913 943 913
Size: 30 4145
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 551 921 551
Size: 370 3805
BseYI C'CCAG_C
Cuts at: 938 1054 1600 2358 2935 3431 938
Size: 116 546 758 577 496 1682
Fragments arranged by size:
1682 758 577 546 496 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 230 439 200
Size: 30 209 3936
Fragments arranged by size:
3936 209 30
BsiEI CG_ry'CG
Cuts at: 104 1717 1763 2971 104
Size: 1613 46 1208 1308
Fragments arranged by size:
1613 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 901 912 1189 1967 2157 2949 901
Size: 11 277 778 190 792 2127
Fragments arranged by size:
2127 792 778 277 190 11
BsmI GAATG_Cn'
Cuts at: 191 333 191
Size: 142 4033
Bsp1286I G_dGCh'C
Cuts at: 647 901 912 1083 1189 1800 1893 1967 2157
Size: 254 11 171 106 611 93 74 190
Cuts at: 2157 2219 2949 647
Size: 62 730 1873
Fragments arranged by size:
1873 730 611 254 190 171 106 93
74 62 11
BspEI T'CCGG_A
Cuts at: 3395 3395
Size: 4175
BspHI T'CATG_A
Cuts at: 3351 3351
Size: 4175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 418 1225 1439 1741 2122 418
Size: 807 214 302 381 2471
Fragments arranged by size:
2471 807 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2467 2521 4005 4170 50
Size: 2417 54 1484 165 55
Fragments arranged by size:
2417 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2087 2087
Size: 4175
BsrFI r'CCGG_y
Cuts at: 234 833 2173 2354 2563 234
Size: 599 1340 181 209 1846
Fragments arranged by size:
1846 1340 599 209 181
BssSI C'ACGA_G
Cuts at: 880 2446 2804 880
Size: 1566 358 2251
Fragments arranged by size:
2251 1566 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1552 1552
Size: 4175
BstBI TT'CG_AA
Cuts at: 2536 2536
Size: 4175
BstYI r'GATC_y
Cuts at: 1 983 1200 1667 1690 2025 2271 3283 3369
Size: 982 217 467 23 335 246 1012 86
Cuts at: 3369 3381 3727 1
Size: 12 346 449
Fragments arranged by size:
1012 982 467 449 346 335 246 217
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 659 659
Size: 4175
BtgI C'CryG_G
Cuts at: 107 467 987 107
Size: 360 520 3295
Fragments arranged by size:
3295 520 360
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 560 2125 2306 2469 560
Size: 1565 181 163 2266
Fragments arranged by size:
2266 1565 181 163
BtsI GCAGTG_nn'
Cuts at: 910 1418 910
Size: 508 3667
DraI TTT'AAA
Cuts at: 1174 1174
Size: 4175
DrdI GACnn_nn'nnGTC
Cuts at: 348 1881 2739 348
Size: 1533 858 1784
Fragments arranged by size:
1784 1533 858
EaeI y'GGCC_r
Cuts at: 401 470 1760 1934 2325 2352 3697 401
Size: 69 1290 174 391 27 1345 879
Fragments arranged by size:
1345 1290 879 391 174 69 27
EagI C'GGCC_G
Cuts at: 1760 1760
Size: 4175
EarI CTCTTCn'nnn_
Cuts at: 842 2198 2408 842
Size: 1356 210 2609
Fragments arranged by size:
2609 1356 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2383 2693 2839 3985 2383
Size: 310 146 1146 2573
Fragments arranged by size:
2573 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1187 1187
Size: 4175
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 176 753 964 1071 1117 2000 2432 3179 3447
Size: 577 211 107 46 883 432 747 268
Cuts at: 3447 176
Size: 904
Fragments arranged by size:
904 883 747 577 432 268 211 107
46
EcoO109I rG'GnC_Cy
Cuts at: 1156 1314 1156
Size: 158 4017
EcoRI G'AATT_C
Cuts at: 1179 1179
Size: 4175
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2194 2194
Size: 4175
FspI TGC'GCA
Cuts at: 1956 1956
Size: 4175
HaeII r_GCGC'y
Cuts at: 516 534 1002 1609 1857 2879 3858 516
Size: 18 468 607 248 1022 979 833
Fragments arranged by size:
1022 979 833 607 468 248 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2017 2047 2049 2079 2017
Size: 30 2 30 4113
Fragments arranged by size:
4113 30 30 2
HincII GTy'rAC
Cuts at: 1214 1214
Size: 4175
KasI G'GCGC_C
Cuts at: 1605 1853 1605
Size: 248 3927
KpnI G_GTAC'C
Cuts at: 1195 1195
Size: 4175
MluI A'CGCG_T
Cuts at: 3608 3608
Size: 4175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2846 3030 2846
Size: 184 3991
MscI TGG'CCA
Cuts at: 403 472 1936 403
Size: 69 1464 2642
Fragments arranged by size:
2642 1464 69
MslI CAynn'nnrTG
Cuts at: 2164 2291 2164
Size: 127 4048
MspA1I CmG'CkG
Cuts at: 423 1286 1600 1960 2973 3218 423
Size: 863 314 360 1013 245 1380
Fragments arranged by size:
1380 1013 863 360 314 245
NaeI GCC'GGC
Cuts at: 2356 2356
Size: 4175
NarI GG'CG_CC
Cuts at: 1606 1854 1606
Size: 248 3927
NcoI C'CATG_G
Cuts at: 107 467 107
Size: 360 3815
NgoMIV G'CCGG_C
Cuts at: 2354 2354
Size: 4175
NspI r_CATG'y
Cuts at: 702 1228 1728 2635 702
Size: 526 500 907 2242
Fragments arranged by size:
2242 907 526 500
PasI CC'CwG_GG
Cuts at: 892 892
Size: 4175
PciI A'CATG_T
Cuts at: 698 2631 698
Size: 1933 2242
PflMI CCAn_nnn'nTGG
Cuts at: 961 961
Size: 4175
PmeI GTTT'AAAC
Cuts at: 1174 1174
Size: 4175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3314 3346 3314
Size: 32 4143
PshAI GACnn'nnGTC
Cuts at: 164 164
Size: 4175
PstI C_TGCA'G
Cuts at: 1222 1907 1222
Size: 685 3490
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4175
PvuII CAG'CTG
Cuts at: 423 1600 1960 423
Size: 1177 360 2638
Fragments arranged by size:
2638 1177 360
RsrII CG'GwC_CG
Cuts at: 2370 2370
Size: 4175
SacI G_AGCT'C
Cuts at: 1189 1189
Size: 4175
SalI G'TCGA_C
Cuts at: 1212 1212
Size: 4175
SapI GCTCTTCn'nnn_
Cuts at: 2198 2408 2198
Size: 210 3965
SbfI CC_TGCA'GG
Cuts at: 1222 1222
Size: 4175
ScaI AGT'ACT
Cuts at: 3692 3692
Size: 4175
SfcI C'TryA_G
Cuts at: 33 1218 1903 2896 3087 33
Size: 1185 685 993 191 1121
Fragments arranged by size:
1185 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1607 1855 1607
Size: 248 3927
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4175
SgrAI Cr'CCGG_yG
Cuts at: 234 234
Size: 4175
SmaI CCC'GGG
Cuts at: 1197 1197
Size: 4175
SmlI C'TyrA_G
Cuts at: 1039 1330 2245 2737 2999 1039
Size: 291 915 492 262 2215
Fragments arranged by size:
2215 915 492 291 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4175
SphI G_CATG'C
Cuts at: 1228 1728 1228
Size: 500 3675
SspI AAT'ATT
Cuts at: 970 970
Size: 4175
StuI AGG'CCT
Cuts at: 1030 1030
Size: 4175
StyI C'CwwG_G
Cuts at: 107 467 1031 1309 1663 107
Size: 360 564 278 354 2619
Fragments arranged by size:
2619 564 360 354 278
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1758 1891 2350 2559 1758
Size: 133 459 209 3374
Fragments arranged by size:
3374 459 209 133
TatI w'GTAC_w
Cuts at: 3690 3690
Size: 4175
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1084 1116 3692 3724 1084
Size: 32 2576 32 1535
Fragments arranged by size:
2576 1535 32 32
Tth111I GACn'n_nGTC
Cuts at: 1972 1972
Size: 4175
XbaI T'CTAG_A
Cuts at: 1206 1206
Size: 4175
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 257 756 1670 257
Size: 499 914 2762
Fragments arranged by size:
2762 914 499
XmaI C'CCGG_G
Cuts at: 1195 1195
Size: 4175
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PmeI PpiI
PshAI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PfoI
PmlI PpuMI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SnaBI SrfI SwaI XhoI XmnI ZraI