Restriction map for
pF1KE2617
(Circular) (Six-base) MAPSORT of: pF1KE2617.seq Check: 355 from: 1 to: 9671
pF1KE2617 9671 bp
With 182 enzymes: SgfI *
May 17, 2016 15:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4380 5065 4380
Size: 685 8986
AatII G_ACGT'C
Cuts at: 4061 5746 4061
Size: 1685 7986
AccI GT'mk_AC
Cuts at: 6615 6650 6709 6615
Size: 35 59 9577
Fragments arranged by size:
9577 59 35
Acc65I G'GTAC_C
Cuts at: 6687 6687
Size: 9671
AclI AA'CG_TT
Cuts at: 9511 9511
Size: 9671
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 1846 2739 2874 2977 3991 4464 4790 5322
Size: 1392 893 135 103 1014 473 326 532
Cuts at: 5322 5345 5983 7496 7928 8675 8943 454
Size: 23 638 1513 432 747 268 1182
Fragments arranged by size:
1513 1392 1182 1014 893 747 638 532
473 432 326 268 135 103 23
AfeI AGC'GCT
Cuts at: 309 309
Size: 9671
AflII C'TTAA_G
Cuts at: 2707 3016 2707
Size: 309 9362
AflIII A'CryG_T
Cuts at: 7023 8127 9104 7023
Size: 1104 977 7590
Fragments arranged by size:
7590 1104 977
AgeI A'CCGG_T
Cuts at: 8059 8059
Size: 9671
AhdI GACnn_n'nnGTC
Cuts at: 2547 5472 2547
Size: 2925 6746
AleI CACnn'nnGTG
Cuts at: 6344 6344
Size: 9671
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1975 5065 1975
Size: 3090 6581
AlwNI CAG_nnn'CTG
Cuts at: 1377 2214 4329 6438 6976 8543 1377
Size: 837 2115 2109 538 1567 2505
Fragments arranged by size:
2505 2115 2109 1567 837 538
ApaI G_GGCC'C
Cuts at: 3568 5639 6324 3568
Size: 2071 685 6915
Fragments arranged by size:
6915 2071 685
ApaLI G'TGCA_C
Cuts at: 8441 8441
Size: 9671
ApoI r'AATT_y
Cuts at: 882 2232 6675 9024 9280 882
Size: 1350 4443 2349 256 1273
Fragments arranged by size:
4443 2349 1350 1273 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 9671
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9671
AvaI C'yCGr_G
Cuts at: 243 410 1358 5514 5705 6691 243
Size: 167 948 4156 191 986 3223
Fragments arranged by size:
4156 3223 986 948 191 167
AvrII C'CTAG_G
Cuts at: 2964 6097 2964
Size: 3133 6538
BamHI G'GATC_C
Cuts at: 6696 6696
Size: 9671
BanI G'GyrC_C
Cuts at: 1625 5064 5696 6303 6505 6526 6687 7101 7349
Size: 3439 632 607 202 21 161 414 248
Cuts at: 7349 7384 1625
Size: 35 3912
Fragments arranged by size:
3912 3439 632 607 414 248 202 161
35 21
BanII G_rGCy'C
Cuts at: 175 417 974 2614 3568 4562 5639 5804 6061
Size: 242 557 1640 954 994 1077 165 257
Cuts at: 6061 6324 6520 6635 6685 7715 175
Size: 263 196 115 50 1030 2131
Fragments arranged by size:
2131 1640 1077 1030 994 954 557 263
257 242 196 165 115 50
BbeI G_GCGC'C
Cuts at: 1629 6509 6530 7105 7353 1629
Size: 4880 21 575 248 3947
Fragments arranged by size:
4880 3947 575 248 21
BbsI GAAGACnn'nnnn_
Cuts at: 1448 1674 2976 3563 4602 6007 1448
Size: 226 1302 587 1039 1405 5112
Fragments arranged by size:
5112 1405 1302 1039 587 226
BbvCI CC'TCA_GC
Cuts at: 814 1981 2864 5276 6496 814
Size: 1167 883 2412 1220 3989
Fragments arranged by size:
3989 2412 1220 1167 883
BciVI GTATCCnnnnn_n'
Cuts at: 7569 8330 9671 7569
Size: 761 1341 7569
Fragments arranged by size:
7569 1341 761
BclI T'GATC_A
Cuts at: 4649 7191 4649
Size: 2542 7129
BfrBI ATG'CAT
Cuts at: 4096 4096
Size: 9671
BglI GCCn_nnn'nGGC
Cuts at: 208 289 1091 1585 1758 1798 3484 3751 5171
Size: 81 802 494 173 40 1686 267 1420
Cuts at: 5171 5360 5597 6524 208
Size: 189 237 927 3355
Fragments arranged by size:
3355 1686 1420 927 802 494 267 237
189 173 81 40
BglII A'GATC_T
Cuts at: 3152 3590 5024 5265 7186 9223 3152
Size: 438 1434 241 1921 2037 3600
Fragments arranged by size:
3600 2037 1921 1434 438 241
BlpI GC'TnA_GC
Cuts at: 293 1318 2842 4270 5683 6783 293
Size: 1025 1524 1428 1413 1100 3181
Fragments arranged by size:
3181 1524 1428 1413 1100 1025
Bme1580I G_kGCm'C
Cuts at: 2726 3568 4548 4855 5069 5639 6308 6324 7296
Size: 842 980 307 214 570 669 16 972
Cuts at: 7296 7389 8445 2726
Size: 93 1056 3952
Fragments arranged by size:
3952 1056 980 972 842 669 570 307
214 93 16
BmgBI CAC'GTC
Cuts at: 1209 5370 1209
Size: 4161 5510
BmrI ACTGGGnnnn_n'
Cuts at: 1227 1957 2181 6397 6883 6964 7299 9443 1227
Size: 730 224 4216 486 81 335 2144 1455
Fragments arranged by size:
4216 2144 1455 730 486 335 224 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 436 4227 5844 8038 436
Size: 3791 1617 2194 2069
Fragments arranged by size:
3791 2194 2069 1617
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 853 1568 1930 2569 2764 5282 6052 6619 853
Size: 715 362 639 195 2518 770 567 3905
Fragments arranged by size:
3905 2518 770 715 639 567 362 195
Bpu10I CC'TnA_GC
Cuts at: 227 528 814 1981 2864 5276 6055 6496 227
Size: 301 286 1167 883 2412 779 441 3402
Fragments arranged by size:
3402 2412 1167 883 779 441 301 286
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2037 3733 6193 6386 6847 7726 8218 8516 2037
Size: 1696 2460 193 461 879 492 298 3192
Fragments arranged by size:
3192 2460 1696 879 492 461 298 193
BsaI GGTCTCn'nnnn_
Cuts at: 1510 1906 1921 4887 5749 6235 9375 1510
Size: 396 15 2966 862 486 3140 1806
Fragments arranged by size:
3140 2966 1806 862 486 396 15
BsaAI yAC'GTr
Cuts at: 3392 3810 7024 3392
Size: 418 3214 6039
Fragments arranged by size:
6039 3214 418
BsaBI GATnn'nnATC
Cuts at: 6 3595 3643 7185 9222 6
Size: 3589 48 3542 2037 455
Fragments arranged by size:
3589 3542 2037 455 48
BsaHI Gr'CG_yC
Cuts at: 1626 4058 4112 5743 6446 6506 6527 7102 7350
Size: 2432 54 1631 703 60 21 575 248
Cuts at: 7350 8052 9548 1626
Size: 702 1496 1749
Fragments arranged by size:
2432 1749 1631 1496 703 702 575 248
60 54 21
BsaWI w'CCGG_w
Cuts at: 446 853 1592 3245 4289 4433 5135 6860 7381
Size: 407 739 1653 1044 144 702 1725 521
Cuts at: 7381 8059 8333 8480 8891 446
Size: 678 274 147 411 1226
Fragments arranged by size:
1725 1653 1226 1044 739 702 678 521
411 407 274 147 144
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1582 1612 1996 2026 3679 3709 5296 5326 5334
Size: 30 384 30 1653 30 1587 30 8
Cuts at: 5334 5364 5659 5689 5728 5758 5846 5876 6214
Size: 30 295 30 39 30 88 30 338
Cuts at: 6214 6244 1582
Size: 30 5009
Fragments arranged by size:
5009 1653 1587 384 338 295 88 39
30 30 30 30 30 30 30 30
30 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 646 1495 2068 2178 2185 2425 2434 2524 2665
Size: 849 573 110 7 240 9 90 141
Cuts at: 2665 2668 4465 4801 5014 5293 5296 5752 5884
Size: 3 1797 336 213 279 3 456 132
Cuts at: 5884 6055 6481 6640 6643 646
Size: 171 426 159 3 3674
Fragments arranged by size:
3674 1797 849 573 456 426 336 279
240 213 171 159 141 132 110 90
9 7 3 3 3
BseYI C'CCAG_C
Cuts at: 1112 3437 4164 4561 5605 5803 6307 6316 7096
Size: 2325 727 397 1044 198 504 9 780
Cuts at: 7096 7854 8431 8927 1112
Size: 758 577 496 1856
Fragments arranged by size:
2325 1856 1044 780 758 727 577 504
496 397 198 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 898 5419 5683 898
Size: 4521 264 4886
Fragments arranged by size:
4886 4521 264
BsiEI CG_ry'CG
Cuts at: 104 5394 6485 6619 7213 7259 8467 104
Size: 5290 1091 134 594 46 1208 1308
Fragments arranged by size:
5290 1308 1208 1091 594 134 46
BsiHKAI G_wGCw'C
Cuts at: 233 974 1742 1964 2170 6635 6685 7463 7653
Size: 741 768 222 206 4465 50 778 190
Cuts at: 7653 8445 233
Size: 792 1459
Fragments arranged by size:
4465 1459 792 778 768 741 222 206
190 50
BsmI GAATG_Cn'
Cuts at: 204 3376 204
Size: 3172 6499
BsmBI CGTCTCn'nnnn_
Cuts at: 3504 5734 5750 6297 3504
Size: 2230 16 547 6878
Fragments arranged by size:
6878 2230 547 16
Bsp1286I G_dGCh'C
Cuts at: 175 233 417 974 1742 1964 2170 2614 2726
Size: 58 184 557 768 222 206 444 112
Cuts at: 2726 3568 4548 4562 4855 5069 5639 5804 6061
Size: 842 980 14 293 214 570 165 257
Cuts at: 6061 6308 6324 6520 6635 6685 7296 7389 7463
Size: 247 16 196 115 50 611 93 74
Cuts at: 7463 7653 7715 8445 175
Size: 190 62 730 1401
Fragments arranged by size:
1401 980 842 768 730 611 570 557
444 293 257 247 222 214 206 196
190 184 165 115 112 93 74 62
58 50 16 14
BspEI T'CCGG_A
Cuts at: 446 853 3245 4289 5135 8891 446
Size: 407 2392 1044 846 3756 1226
Fragments arranged by size:
3756 2392 1226 1044 846 407
BspHI T'CATG_A
Cuts at: 3635 8847 3635
Size: 5212 4459
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1006 3095 4380 5065 6721 6935 7237 7618 1006
Size: 2089 1285 685 1656 214 302 381 3059
Fragments arranged by size:
3059 2089 1656 1285 685 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3536 5918 7963 8017 9501 9666 50
Size: 3486 2382 2045 54 1484 165 55
Fragments arranged by size:
3486 2382 2045 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 321 1727 2357 2976 3977 7583 321
Size: 1406 630 619 1001 3606 2409
Fragments arranged by size:
3606 2409 1406 1001 630 619
BsrFI r'CCGG_y
Cuts at: 1628 2487 5347 5533 5596 7669 7850 8059 1628
Size: 859 2860 186 63 2073 181 209 3240
Fragments arranged by size:
3240 2860 2073 859 209 186 181 63
BsrGI T'GTAC_A
Cuts at: 131 1832 3332 131
Size: 1701 1500 6470
Fragments arranged by size:
6470 1701 1500
BssHII G'CGCG_C
Cuts at: 6603 6605 6603
Size: 2 9669
BssSI C'ACGA_G
Cuts at: 1998 5485 7942 8300 1998
Size: 3487 2457 358 3369
Fragments arranged by size:
3487 3369 2457 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1100 7048 1100
Size: 5948 3723
BstBI TT'CG_AA
Cuts at: 3509 8032 3509
Size: 4523 5148
BstEII G'GTnAC_C
Cuts at: 4803 5088 6423 4803
Size: 285 1335 8051
Fragments arranged by size:
8051 1335 285
BstXI CCAn_nnnn'nTGG
Cuts at: 522 1923 2364 3873 6357 522
Size: 1401 441 1509 2484 3836
Fragments arranged by size:
3836 2484 1509 1401 441
BstYI r'GATC_y
Cuts at: 1 1253 1404 1595 2210 2960 3152 3590 5024
Size: 1252 151 191 615 750 192 438 1434
Cuts at: 5024 5243 5265 6405 6696 7163 7186 7521 7767
Size: 219 22 1140 291 467 23 335 246
Cuts at: 7767 8779 8865 8877 9223 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1434 1252 1140 1012 750 615 467 449
438 346 335 291 246 219 192 191
151 86 23 22 12
Bsu36I CC'TnA_GG
Cuts at: 3184 4960 3184
Size: 1776 7895
BtgI C'CryG_G
Cuts at: 107 392 899 1071 1190 3956 4275 5631 5723
Size: 285 507 172 119 2766 319 1356 92
Cuts at: 5723 6339 107
Size: 616 3439
Fragments arranged by size:
3439 2766 1356 616 507 319 285 172
119 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 903 2043 3609 7621 7802 7965 903
Size: 1140 1566 4012 181 163 2609
Fragments arranged by size:
4012 2609 1566 1140 181 163
BtsI GCAGTG_nn'
Cuts at: 1087 2498 2726 5221 5802 6050 6222 6351 6914
Size: 1411 228 2495 581 248 172 129 563
Cuts at: 6914 1087
Size: 3844
Fragments arranged by size:
3844 2495 1411 581 563 248 228 172
129
ClaI AT'CG_AT
Cuts at: 1448 1448
Size: 9671
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 4920 4955 4920
Size: 35 9636
DraI TTT'AAA
Cuts at: 635 4743 6670 635
Size: 4108 1927 3636
Fragments arranged by size:
4108 3636 1927
DraIII CAC_nnn'GTG
Cuts at: 1100 4591 4863 1100
Size: 3491 272 5908
Fragments arranged by size:
5908 3491 272
DrdI GACnn_nn'nnGTC
Cuts at: 1888 4915 6646 7377 8235 1888
Size: 3027 1731 731 858 3324
Fragments arranged by size:
3324 3027 1731 858 731
EaeI y'GGCC_r
Cuts at: 269 395 2354 2441 3517 3875 3891 5391 6440
Size: 126 1959 87 1076 358 16 1500 1049
Cuts at: 6440 6482 7256 7430 7821 7848 9193 269
Size: 42 774 174 391 27 1345 747
Fragments arranged by size:
1959 1500 1345 1076 1049 774 747 391
358 174 126 87 42 27 16
EagI C'GGCC_G
Cuts at: 5391 6482 7256 5391
Size: 1091 774 7806
Fragments arranged by size:
7806 1091 774
EarI CTCTTCn'nnn_
Cuts at: 979 1394 1873 2086 2092 2320 2411 2636 2833
Size: 415 479 213 6 228 91 225 197
Cuts at: 2833 3322 3496 3943 3955 4483 5896 6578 7694
Size: 489 174 447 12 528 1413 682 1116
Cuts at: 7694 7904 979
Size: 210 2746
Fragments arranged by size:
2746 1413 1116 682 528 489 479 447
415 228 225 213 210 197 174 91
12 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3382 4225 4833 7879 8189 8335 9481 3382
Size: 843 608 3046 310 146 1146 3572
Fragments arranged by size:
3572 3046 1146 843 608 310 146
EcoICRI GAG'CTC
Cuts at: 972 6633 6683 972
Size: 5661 50 3960
Fragments arranged by size:
5661 3960 50
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 853 1568 1846 1930 2569 2739 2764 2874
Size: 399 715 278 84 639 170 25 110
Cuts at: 2874 2977 3991 4464 4790 5282 5322 5345 5983
Size: 103 1014 473 326 492 40 23 638
Cuts at: 5983 6052 6619 7496 7928 8675 8943 454
Size: 69 567 877 432 747 268 1182
Fragments arranged by size:
1182 1014 877 747 715 639 638 567
492 473 432 399 326 278 268 170
110 103 84 69 40 25 23
EcoNI CCTnn'n_nnAGG
Cuts at: 2889 5122 5184 2889
Size: 2233 62 7376
Fragments arranged by size:
7376 2233 62
EcoO109I rG'GnC_Cy
Cuts at: 452 818 1791 2868 3179 3564 3565 4326 5163
Size: 366 973 1077 311 385 1 761 837
Cuts at: 5163 5636 5939 6320 6321 6810 452
Size: 473 303 381 1 489 3313
Fragments arranged by size:
3313 1077 973 837 761 489 473 385
381 366 311 303 1 1
EcoRI G'AATT_C
Cuts at: 882 2232 6675 882
Size: 1350 4443 3878
Fragments arranged by size:
4443 3878 1350
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2157 3474 4017 7690 2157
Size: 1317 543 3673 4138
Fragments arranged by size:
4138 3673 1317 543
FspI TGC'GCA
Cuts at: 5218 7452 5218
Size: 2234 7437
HaeII r_GCGC'y
Cuts at: 311 814 1629 5402 5430 6272 6509 6530 7105
Size: 503 815 3773 28 842 237 21 575
Cuts at: 7105 7353 8375 9354 311
Size: 248 1022 979 628
Fragments arranged by size:
3773 1022 979 842 815 628 575 503
248 237 28 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1507 1539 1541 1573 2512 2533 2544 2565 5240
Size: 32 2 32 939 21 11 21 2675
Cuts at: 5240 5272 5296 5328 5458 5464 5490 5496 5728
Size: 32 24 32 130 6 26 6 232
Cuts at: 5728 5760 7513 7543 7545 7575 1507
Size: 32 1753 30 2 30 3603
Fragments arranged by size:
3603 2675 1753 939 232 130 32 32
32 32 32 30 30 26 24 21
21 11 6 6 2 2
HincII GTy'rAC
Cuts at: 1530 3411 3816 6616 6710 1530
Size: 1881 405 2800 94 4491
Fragments arranged by size:
4491 2800 1881 405 94
KasI G'GCGC_C
Cuts at: 1625 6505 6526 7101 7349 1625
Size: 4880 21 575 248 3947
Fragments arranged by size:
4880 3947 575 248 21
KpnI G_GTAC'C
Cuts at: 6691 6691
Size: 9671
MfeI C'AATT_G
Cuts at: 516 516
Size: 9671
MluI A'CGCG_T
Cuts at: 9104 9104
Size: 9671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 185 587 602 3450 5271 5564 5714 8342 8526
Size: 402 15 2848 1821 293 150 2628 184
Cuts at: 8526 185
Size: 1330
Fragments arranged by size:
2848 2628 1821 1330 402 293 184 150
15
MscI TGG'CCA
Cuts at: 2356 2443 3519 3877 7432 2356
Size: 87 1076 358 3555 4595
Fragments arranged by size:
4595 3555 1076 358 87
MslI CAynn'nnrTG
Cuts at: 668 1189 1501 1921 1957 2362 3649 4733 4796
Size: 521 312 420 36 405 1287 1084 63
Cuts at: 4796 4891 5659 5821 6344 7660 7787 668
Size: 95 768 162 523 1316 127 2552
Fragments arranged by size:
2552 1316 1287 1084 768 523 521 420
405 312 162 127 95 63 36
MspA1I CmG'CkG
Cuts at: 214 348 3437 4168 5275 5317 5433 6500 6782
Size: 134 3089 731 1107 42 116 1067 282
Cuts at: 6782 7096 7456 8469 8714 214
Size: 314 360 1013 245 1171
Fragments arranged by size:
3089 1171 1107 1067 1013 731 360 314
282 245 134 116 42
NaeI GCC'GGC
Cuts at: 2489 5598 7852 2489
Size: 3109 2254 4308
Fragments arranged by size:
4308 3109 2254
NarI GG'CG_CC
Cuts at: 1626 6506 6527 7102 7350 1626
Size: 4880 21 575 248 3947
Fragments arranged by size:
4880 3947 575 248 21
NcoI C'CATG_G
Cuts at: 107 899 1190 5723 107
Size: 792 291 4533 4055
Fragments arranged by size:
4533 4055 792 291
NgoMIV G'CCGG_C
Cuts at: 2487 5596 7850 2487
Size: 3109 2254 4308
Fragments arranged by size:
4308 3109 2254
NsiI A_TGCA'T
Cuts at: 4098 4098
Size: 9671
NspI r_CATG'y
Cuts at: 1506 3220 3936 4530 4635 4896 6724 7224 8131
Size: 1714 716 594 105 261 1828 500 907
Cuts at: 8131 1506
Size: 3046
Fragments arranged by size:
3046 1828 1714 907 716 594 500 261
105
PasI CC'CwG_GG
Cuts at: 5574 6325 5574
Size: 751 8920
PciI A'CATG_T
Cuts at: 8127 8127
Size: 9671
PflMI CCAn_nnn'nTGG
Cuts at: 4731 6438 4731
Size: 1707 7964
PfoI T'CCnGG_A
Cuts at: 1907 3080 5123 6635 1907
Size: 1173 2043 1512 4943
Fragments arranged by size:
4943 2043 1512 1173
PmeI GTTT'AAAC
Cuts at: 6670 6670
Size: 9671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8810 8842 8810
Size: 32 9639
PpuMI rG'GwC_Cy
Cuts at: 2868 2868
Size: 9671
PsiI TTA'TAA
Cuts at: 709 709
Size: 9671
PspOMI G'GGCC_C
Cuts at: 3564 5635 6320 3564
Size: 2071 685 6915
Fragments arranged by size:
6915 2071 685
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3826 3858 3826
Size: 32 9639
PstI C_TGCA'G
Cuts at: 1648 1850 3287 5076 5207 5797 6553 6718 7403
Size: 202 1437 1789 131 590 756 165 685
Cuts at: 7403 1648
Size: 3916
Fragments arranged by size:
3916 1789 1437 756 685 590 202 165
131
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9671
PvuII CAG'CTG
Cuts at: 3437 4168 7096 7456 3437
Size: 731 2928 360 5652
Fragments arranged by size:
5652 2928 731 360
RsrII CG'GwC_CG
Cuts at: 7866 7866
Size: 9671
SacI G_AGCT'C
Cuts at: 974 6635 6685 974
Size: 5661 50 3960
Fragments arranged by size:
5661 3960 50
SalI G'TCGA_C
Cuts at: 6614 6708 6614
Size: 94 9577
SapI GCTCTTCn'nnn_
Cuts at: 979 3322 3943 7694 7904 979
Size: 2343 621 3751 210 2746
Fragments arranged by size:
3751 2746 2343 621 210
SbfI CC_TGCA'GG
Cuts at: 1850 5076 6718 1850
Size: 3226 1642 4803
Fragments arranged by size:
4803 3226 1642
ScaI AGT'ACT
Cuts at: 3173 5131 9188 3173
Size: 1958 4057 3656
Fragments arranged by size:
4057 3656 1958
SexAI A'CCwGG_T
Cuts at: 2024 5057 2024
Size: 3033 6638
SfcI C'TryA_G
Cuts at: 33 1644 1846 2633 3283 5072 5203 5793 6549
Size: 1611 202 787 650 1789 131 590 756
Cuts at: 6549 6714 7399 8392 8583 33
Size: 165 685 993 191 1121
Fragments arranged by size:
1789 1611 1121 993 787 756 685 650
590 202 191 165 131
SfiI GGCCn_nnn'nGGCC
Cuts at: 1585 1798 1585
Size: 213 9458
SfoI GGC'GCC
Cuts at: 1627 6507 6528 7103 7351 1627
Size: 4880 21 575 248 3947
Fragments arranged by size:
4880 3947 575 248 21
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9671
SgrAI Cr'CCGG_yG
Cuts at: 1628 1628
Size: 9671
SmaI CCC'GGG
Cuts at: 245 1360 5707 6693 245
Size: 1115 4347 986 3223
Fragments arranged by size:
4347 3223 1115 986
SmlI C'TyrA_G
Cuts at: 2016 2707 3016 3748 6172 6401 6826 7741 8233
Size: 691 309 732 2424 229 425 915 492
Cuts at: 8233 8495 2016
Size: 262 3192
Fragments arranged by size:
3192 2424 915 732 691 492 425 309
262 229
SpeI A'CTAG_T
Cuts at: 67 2989 67
Size: 2922 6749
SphI G_CATG'C
Cuts at: 1506 4530 6724 7224 1506
Size: 3024 2194 500 3953
Fragments arranged by size:
3953 3024 2194 500
StuI AGG'CCT
Cuts at: 1852 4501 5791 6053 6102 1852
Size: 2649 1290 262 49 5421
Fragments arranged by size:
5421 2649 1290 262 49
StyI C'CwwG_G
Cuts at: 107 153 899 1190 1352 2504 2964 3203 3992
Size: 46 746 291 162 1152 460 239 789
Cuts at: 3992 4716 4775 5723 6097 6805 7159 107
Size: 724 59 948 374 708 354 2619
Fragments arranged by size:
2619 1152 948 789 746 724 708 460
374 354 291 239 162 59 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1534 5993 6445 6584 6633 7254 7387 7846 8055
Size: 4459 452 139 49 621 133 459 209
Cuts at: 8055 1534
Size: 3150
Fragments arranged by size:
4459 3150 621 459 452 209 139 133
49
TatI w'GTAC_w
Cuts at: 131 1214 1293 1832 3171 3332 3695 3794 5129
Size: 1083 79 539 1339 161 363 99 1335
Cuts at: 5129 9186 131
Size: 4057 616
Fragments arranged by size:
4057 1339 1335 1083 616 539 363 161
99 79
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 142 174 788 820 1582 1614 5334 5366 5659
Size: 32 614 32 762 32 3720 32 293
Cuts at: 5659 5691 6140 6172 6214 6246 9188 9220 142
Size: 32 449 32 42 32 2942 32 593
Fragments arranged by size:
3720 2942 762 614 593 449 293 42
32 32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1521 2872 2980 7468 1521
Size: 1351 108 4488 3724
Fragments arranged by size:
4488 3724 1351 108
XbaI T'CTAG_A
Cuts at: 6702 6702
Size: 9671
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 579 963 2571 6357 6435 6468 7166 579
Size: 384 1608 3786 78 33 698 3084
Fragments arranged by size:
3786 3084 1608 698 384 78 33
XmaI C'CCGG_G
Cuts at: 243 1358 5705 6691 243
Size: 1115 4347 986 3223
Fragments arranged by size:
4347 3223 1115 986
XmnI GAAnn'nnTTC
Cuts at: 1639 2347 3070 3646 6078 1639
Size: 708 723 576 2432 5232
Fragments arranged by size:
5232 2432 723 708 576
ZraI GAC'GTC
Cuts at: 4059 5744 4059
Size: 1685 7986
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI BaeI BcgI BmtI BsiWI BstZ17I EcoRV
FseI FspAI HindIII HpaI NdeI NheI NotI NruI
PacI PmlI PshAI PspXI SacII SanDI SnaBI SrfI
SspI SwaI XhoI