Restriction map for
pF1KE2626
(Circular) (Six-base) MAPSORT of: pF1KE2626.seq Check: 4654 from: 1 to: 4019
pF1KE2626 4019 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 802 802
Size: 4019
AccI GT'mk_AC
Cuts at: 1057 1057
Size: 4019
Acc65I G'GTAC_C
Cuts at: 1035 1035
Size: 4019
AclI AA'CG_TT
Cuts at: 3859 3859
Size: 4019
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 388 560 566 1844 2276 3023 3291 134
Size: 254 172 6 1278 432 747 268 862
Fragments arranged by size:
1278 862 747 432 268 254 172 6
AflII C'TTAA_G
Cuts at: 454 454
Size: 4019
AflIII A'CryG_T
Cuts at: 1371 2475 3452 1371
Size: 1104 977 1938
Fragments arranged by size:
1938 1104 977
AgeI A'CCGG_T
Cuts at: 2407 2407
Size: 4019
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 293 293
Size: 4019
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 140 172 275 307 140
Size: 32 103 32 3852
Fragments arranged by size:
3852 103 32 32
AlwNI CAG_nnn'CTG
Cuts at: 248 1324 2891 248
Size: 1076 1567 1376
Fragments arranged by size:
1567 1376 1076
ApaLI G'TGCA_C
Cuts at: 815 2789 815
Size: 1974 2045
ApoI r'AATT_y
Cuts at: 163 418 590 687 1023 3372 3628 163
Size: 255 172 97 336 2349 256 554
Fragments arranged by size:
2349 554 336 256 255 172 97
AseI AT'TA_AT
Cuts at: 20 20
Size: 4019
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4019
AvaI C'yCGr_G
Cuts at: 1039 1039
Size: 4019
BamHI G'GATC_C
Cuts at: 1044 1044
Size: 4019
BanI G'GyrC_C
Cuts at: 1035 1449 1697 1732 1035
Size: 414 248 35 3322
Fragments arranged by size:
3322 414 248 35
BanII G_rGCy'C
Cuts at: 1033 2063 1033
Size: 1030 2989
BbeI G_GCGC'C
Cuts at: 1453 1701 1453
Size: 248 3771
BciVI GTATCCnnnnn_n'
Cuts at: 1917 2678 4019 1917
Size: 761 1341 1917
Fragments arranged by size:
1917 1341 761
BclI T'GATC_A
Cuts at: 1539 1539
Size: 4019
BglII A'GATC_T
Cuts at: 981 1534 3571 981
Size: 553 2037 1429
Fragments arranged by size:
2037 1429 553
BlpI GC'TnA_GC
Cuts at: 1131 1131
Size: 4019
Bme1580I G_kGCm'C
Cuts at: 819 1644 1737 2793 819
Size: 825 93 1056 2045
Fragments arranged by size:
2045 1056 825 93
BmrI ACTGGGnnnn_n'
Cuts at: 1231 1312 1647 3791 1231
Size: 81 335 2144 1459
Fragments arranged by size:
2144 1459 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 584 2386 584
Size: 1802 2217
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 581 1195 2074 2566 2864 581
Size: 614 879 492 298 1736
Fragments arranged by size:
1736 879 614 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3723 3723
Size: 4019
BsaAI yAC'GTr
Cuts at: 1372 1372
Size: 4019
BsaBI GATnn'nnATC
Cuts at: 6 178 1533 3570 6
Size: 172 1355 2037 455
Fragments arranged by size:
2037 1355 455 172
BsaHI Gr'CG_yC
Cuts at: 1450 1698 2400 3896 1450
Size: 248 702 1496 1573
Fragments arranged by size:
1573 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1208 1729 2407 2681 2828 3239 1208
Size: 521 678 274 147 411 1988
Fragments arranged by size:
1988 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 988 1444 2202 2779 3275 988
Size: 456 758 577 496 1732
Fragments arranged by size:
1732 758 577 496 456
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 261 872 261
Size: 611 3408
BsiEI CG_ry'CG
Cuts at: 104 1561 1607 2815 104
Size: 1457 46 1208 1308
Fragments arranged by size:
1457 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 819 1033 1811 2001 2793 819
Size: 214 778 190 792 2045
Fragments arranged by size:
2045 792 778 214 190
Bsp1286I G_dGCh'C
Cuts at: 819 1033 1644 1737 1811 2001 2063 2793 819
Size: 214 611 93 74 190 62 730 2045
Fragments arranged by size:
2045 730 611 214 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3239 3239
Size: 4019
BspHI T'CATG_A
Cuts at: 3195 3195
Size: 4019
BspMI ACCTGCnnnn'nnnn_
Cuts at: 802 1069 1283 1585 1966 802
Size: 267 214 302 381 2855
Fragments arranged by size:
2855 381 302 267 214
BsrBI CCG'CTC
Cuts at: 50 2311 2365 3849 4014 50
Size: 2261 54 1484 165 55
Fragments arranged by size:
2261 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 713 1931 713
Size: 1218 2801
BsrFI r'CCGG_y
Cuts at: 2017 2198 2407 2017
Size: 181 209 3629
Fragments arranged by size:
3629 209 181
BssSI C'ACGA_G
Cuts at: 2290 2648 2290
Size: 358 3661
BstAPI GCAn_nnn'nTGC
Cuts at: 880 1396 880
Size: 516 3503
BstBI TT'CG_AA
Cuts at: 2380 2380
Size: 4019
BstYI r'GATC_y
Cuts at: 1 381 505 981 1044 1511 1534 1869 2115
Size: 380 124 476 63 467 23 335 246
Cuts at: 2115 3127 3213 3225 3571 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 476 467 449 380 346 335 246
124 86 63 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1969 2150 2313 1969
Size: 181 163 3675
Fragments arranged by size:
3675 181 163
BtsI GCAGTG_nn'
Cuts at: 1017 1262 1017
Size: 245 3774
DraI TTT'AAA
Cuts at: 1018 1018
Size: 4019
DraIII CAC_nnn'GTG
Cuts at: 895 895
Size: 4019
DrdI GACnn_nn'nnGTC
Cuts at: 1725 2583 1725
Size: 858 3161
EaeI y'GGCC_r
Cuts at: 1604 1778 2169 2196 3541 1604
Size: 174 391 27 1345 2082
Fragments arranged by size:
2082 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1604 1604
Size: 4019
EarI CTCTTCn'nnn_
Cuts at: 260 364 746 2042 2252 260
Size: 104 382 1296 210 2027
Fragments arranged by size:
2027 1296 382 210 104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2227 2537 2683 3829 2227
Size: 310 146 1146 2417
Fragments arranged by size:
2417 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1031 1031
Size: 4019
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 388 560 566 1844 2276 3023 3291 134
Size: 254 172 6 1278 432 747 268 862
Fragments arranged by size:
1278 862 747 432 268 254 172 6
EcoNI CCTnn'n_nnAGG
Cuts at: 809 809
Size: 4019
EcoO109I rG'GnC_Cy
Cuts at: 841 1158 841
Size: 317 3702
EcoRI G'AATT_C
Cuts at: 687 1023 687
Size: 336 3683
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2038 2038
Size: 4019
FspI TGC'GCA
Cuts at: 1800 1800
Size: 4019
HaeII r_GCGC'y
Cuts at: 1453 1701 2723 3702 1453
Size: 248 1022 979 1770
Fragments arranged by size:
1770 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 296 328 1861 1891 1893 1923 296
Size: 32 1533 30 2 30 2392
Fragments arranged by size:
2392 1533 32 30 30 2
HincII GTy'rAC
Cuts at: 729 1058 729
Size: 329 3690
KasI G'GCGC_C
Cuts at: 1449 1697 1449
Size: 248 3771
KpnI G_GTAC'C
Cuts at: 1039 1039
Size: 4019
MluI A'CGCG_T
Cuts at: 3452 3452
Size: 4019
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 450 2690 2874 450
Size: 2240 184 1595
Fragments arranged by size:
2240 1595 184
MscI TGG'CCA
Cuts at: 1780 1780
Size: 4019
MslI CAynn'nnrTG
Cuts at: 275 2008 2135 275
Size: 1733 127 2159
Fragments arranged by size:
2159 1733 127
MspA1I CmG'CkG
Cuts at: 886 974 1130 1444 1804 2817 3062 886
Size: 88 156 314 360 1013 245 1843
Fragments arranged by size:
1843 1013 360 314 245 156 88
NaeI GCC'GGC
Cuts at: 2200 2200
Size: 4019
NarI GG'CG_CC
Cuts at: 1450 1698 1450
Size: 248 3771
NgoMIV G'CCGG_C
Cuts at: 2198 2198
Size: 4019
NspI r_CATG'y
Cuts at: 1072 1572 2479 1072
Size: 500 907 2612
Fragments arranged by size:
2612 907 500
PciI A'CATG_T
Cuts at: 2475 2475
Size: 4019
PmeI GTTT'AAAC
Cuts at: 1018 1018
Size: 4019
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3158 3190 3158
Size: 32 3987
PpuMI rG'GwC_Cy
Cuts at: 841 841
Size: 4019
PstI C_TGCA'G
Cuts at: 615 1066 1751 615
Size: 451 685 2883
Fragments arranged by size:
2883 685 451
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4019
PvuII CAG'CTG
Cuts at: 886 974 1444 1804 886
Size: 88 470 360 3101
Fragments arranged by size:
3101 470 360 88
RsrII CG'GwC_CG
Cuts at: 2214 2214
Size: 4019
SacI G_AGCT'C
Cuts at: 1033 1033
Size: 4019
SalI G'TCGA_C
Cuts at: 1056 1056
Size: 4019
SapI GCTCTTCn'nnn_
Cuts at: 260 2042 2252 260
Size: 1782 210 2027
Fragments arranged by size:
2027 1782 210
SbfI CC_TGCA'GG
Cuts at: 1066 1066
Size: 4019
ScaI AGT'ACT
Cuts at: 362 3536 362
Size: 3174 845
SfcI C'TryA_G
Cuts at: 33 443 487 611 1062 1747 2740 2931 33
Size: 410 44 124 451 685 993 191 1121
Fragments arranged by size:
1121 993 685 451 410 191 124 44
SfoI GGC'GCC
Cuts at: 1451 1699 1451
Size: 248 3771
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4019
SmaI CCC'GGG
Cuts at: 1041 1041
Size: 4019
SmlI C'TyrA_G
Cuts at: 454 596 1174 2089 2581 2843 454
Size: 142 578 915 492 262 1630
Fragments arranged by size:
1630 915 578 492 262 142
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4019
SphI G_CATG'C
Cuts at: 1072 1572 1072
Size: 500 3519
SspI AAT'ATT
Cuts at: 222 222
Size: 4019
StyI C'CwwG_G
Cuts at: 1153 1507 1153
Size: 354 3665
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1602 1735 2194 2403 1602
Size: 133 459 209 3218
Fragments arranged by size:
3218 459 209 133
TatI w'GTAC_w
Cuts at: 360 3534 360
Size: 3174 845
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3536 3568 3536
Size: 32 3987
Tth111I GACn'n_nGTC
Cuts at: 724 1816 724
Size: 1092 2927
XbaI T'CTAG_A
Cuts at: 1050 1050
Size: 4019
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1514 1514
Size: 4019
XmaI C'CCGG_G
Cuts at: 1039 1039
Size: 4019
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AleI ApaI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI BpmI
Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI
ZraI