Restriction map for
pF1KE2627
(Circular) (Six-base) MAPSORT of: pF1KE2627.seq Check: 6756 from: 1 to: 3926
pF1KE2627 3926 bp
With 182 enzymes: SgfI *
May 31, 2016 13:40 ..
AccI GT'mk_AC
Cuts at: 964 964
Size: 3926
Acc65I G'GTAC_C
Cuts at: 910 942 910
Size: 32 3894
AclI AA'CG_TT
Cuts at: 3766 3766
Size: 3926
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 838 1751 2183 2930 3198 838
Size: 913 432 747 268 1566
Fragments arranged by size:
1566 913 747 432 268
AflIII A'CryG_T
Cuts at: 1278 2382 3359 1278
Size: 1104 977 1845
Fragments arranged by size:
1845 1104 977
AgeI A'CCGG_T
Cuts at: 2314 2314
Size: 3926
AlwNI CAG_nnn'CTG
Cuts at: 219 282 390 900 1231 2798 219
Size: 63 108 510 331 1567 1347
Fragments arranged by size:
1567 1347 510 331 108 63
ApaI G_GGCC'C
Cuts at: 303 303
Size: 3926
ApaLI G'TGCA_C
Cuts at: 2696 2696
Size: 3926
ApoI r'AATT_y
Cuts at: 697 930 3279 3535 697
Size: 233 2349 256 1088
Fragments arranged by size:
2349 1088 256 233
AseI AT'TA_AT
Cuts at: 20 20
Size: 3926
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3926
AvaI C'yCGr_G
Cuts at: 576 946 576
Size: 370 3556
BamHI G'GATC_C
Cuts at: 632 951 632
Size: 319 3607
BanI G'GyrC_C
Cuts at: 265 580 739 910 942 1356 1604 1639 265
Size: 315 159 171 32 414 248 35 2552
Fragments arranged by size:
2552 414 315 248 171 159 35 32
BanII G_rGCy'C
Cuts at: 119 303 512 754 940 1970 119
Size: 184 209 242 186 1030 2075
Fragments arranged by size:
2075 1030 242 209 186 184
BbeI G_GCGC'C
Cuts at: 743 1360 1608 743
Size: 617 248 3061
Fragments arranged by size:
3061 617 248
BbsI GAAGACnn'nnnn_
Cuts at: 171 708 171
Size: 537 3389
BciVI GTATCCnnnnn_n'
Cuts at: 1824 2585 3926 1824
Size: 761 1341 1824
Fragments arranged by size:
1824 1341 761
BclI T'GATC_A
Cuts at: 1446 1446
Size: 3926
BglI GCCn_nnn'nGGC
Cuts at: 338 338
Size: 3926
BglII A'GATC_T
Cuts at: 1441 3478 1441
Size: 2037 1889
BlpI GC'TnA_GC
Cuts at: 1038 1038
Size: 3926
Bme1580I G_kGCm'C
Cuts at: 303 583 1551 1644 2700 303
Size: 280 968 93 1056 1529
Fragments arranged by size:
1529 1056 968 280 93
BmgBI CAC'GTC
Cuts at: 348 348
Size: 3926
BmrI ACTGGGnnnn_n'
Cuts at: 720 1138 1219 1554 3698 720
Size: 418 81 335 2144 948
Fragments arranged by size:
2144 948 418 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 138 903 2293 138
Size: 765 1390 1771
Fragments arranged by size:
1771 1390 765
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 120 662 847 120
Size: 542 185 3199
Fragments arranged by size:
3199 542 185
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1102 1981 2473 2771 1102
Size: 879 492 298 2257
Fragments arranged by size:
2257 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3630 3630
Size: 3926
BsaAI yAC'GTr
Cuts at: 375 771 1279 375
Size: 396 508 3022
Fragments arranged by size:
3022 508 396
BsaBI GATnn'nnATC
Cuts at: 6 1440 3477 6
Size: 1434 2037 455
Fragments arranged by size:
2037 1434 455
BsaHI Gr'CG_yC
Cuts at: 740 1357 1605 2307 3803 740
Size: 617 248 702 1496 863
Fragments arranged by size:
1496 863 702 617 248
BsaWI w'CCGG_w
Cuts at: 1115 1636 2314 2588 2735 3146 1115
Size: 521 678 274 147 411 1895
Fragments arranged by size:
1895 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 160 748 862 160
Size: 588 114 3224
Fragments arranged by size:
3224 588 114
BseYI C'CCAG_C
Cuts at: 383 390 1351 2109 2686 3182 383
Size: 7 961 758 577 496 1127
Fragments arranged by size:
1127 961 758 577 496 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 868 868
Size: 3926
BsiEI CG_ry'CG
Cuts at: 104 1468 1514 2722 104
Size: 1364 46 1208 1308
Fragments arranged by size:
1364 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 119 512 536 940 1718 1908 2700 119
Size: 393 24 404 778 190 792 1345
Fragments arranged by size:
1345 792 778 404 393 190 24
BsmBI CGTCTCn'nnnn_
Cuts at: 354 354
Size: 3926
Bsp1286I G_dGCh'C
Cuts at: 119 303 512 536 583 754 940 1551 1644
Size: 184 209 24 47 171 186 611 93
Cuts at: 1644 1718 1908 1970 2700 119
Size: 74 190 62 730 1345
Fragments arranged by size:
1345 730 611 209 190 186 184 171
93 74 62 47 24
BspEI T'CCGG_A
Cuts at: 3146 3146
Size: 3926
BspHI T'CATG_A
Cuts at: 3102 3102
Size: 3926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 976 1190 1492 1873 976
Size: 214 302 381 3029
Fragments arranged by size:
3029 381 302 214
BsrBI CCG'CTC
Cuts at: 50 122 412 668 2218 2272 3756 3921 50
Size: 72 290 256 1550 54 1484 165 55
Fragments arranged by size:
1550 1484 290 256 165 72 55 54
BsrDI GCAATG_nn'
Cuts at: 1838 1838
Size: 3926
BsrFI r'CCGG_y
Cuts at: 643 1924 2105 2314 643
Size: 1281 181 209 2255
Fragments arranged by size:
2255 1281 209 181
BssSI C'ACGA_G
Cuts at: 2197 2555 2197
Size: 358 3568
BstAPI GCAn_nnn'nTGC
Cuts at: 531 900 1303 531
Size: 369 403 3154
Fragments arranged by size:
3154 403 369
BstBI TT'CG_AA
Cuts at: 2287 2287
Size: 3926
BstXI CCAn_nnnn'nTGG
Cuts at: 628 628
Size: 3926
BstYI r'GATC_y
Cuts at: 1 632 951 1418 1441 1776 2022 3034 3120
Size: 631 319 467 23 335 246 1012 86
Cuts at: 3120 3132 3478 1
Size: 12 346 449
Fragments arranged by size:
1012 631 467 449 346 335 319 246
86 23 12
BtgI C'CryG_G
Cuts at: 242 444 564 904 242
Size: 202 120 340 3264
Fragments arranged by size:
3264 340 202 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1876 2057 2220 1876
Size: 181 163 3582
Fragments arranged by size:
3582 181 163
BtsI GCAGTG_nn'
Cuts at: 520 818 1169 520
Size: 298 351 3277
Fragments arranged by size:
3277 351 298
DraI TTT'AAA
Cuts at: 925 925
Size: 3926
DrdI GACnn_nn'nnGTC
Cuts at: 457 712 1632 2490 457
Size: 255 920 858 1893
Fragments arranged by size:
1893 920 858 255
EaeI y'GGCC_r
Cuts at: 161 208 401 489 1511 1685 2076 2103 3448
Size: 47 193 88 1022 174 391 27 1345
Cuts at: 3448 161
Size: 639
Fragments arranged by size:
1345 1022 639 391 193 174 88 47
27
EagI C'GGCC_G
Cuts at: 1511 1511
Size: 3926
EarI CTCTTCn'nnn_
Cuts at: 178 517 1949 2159 178
Size: 339 1432 210 1945
Fragments arranged by size:
1945 1432 339 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 770 2134 2444 2590 3736 770
Size: 1364 310 146 1146 960
Fragments arranged by size:
1364 1146 960 310 146
EcoICRI GAG'CTC
Cuts at: 117 510 938 117
Size: 393 428 3105
Fragments arranged by size:
3105 428 393
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 120 662 838 847 1751 2183 2930 3198 120
Size: 542 176 9 904 432 747 268 848
Fragments arranged by size:
904 848 747 542 432 268 176 9
EcoNI CCTnn'n_nnAGG
Cuts at: 135 274 135
Size: 139 3787
EcoO109I rG'GnC_Cy
Cuts at: 216 299 450 822 1065 216
Size: 83 151 372 243 3077
Fragments arranged by size:
3077 372 243 151 83
EcoRI G'AATT_C
Cuts at: 930 930
Size: 3926
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1945 1945
Size: 3926
FspI TGC'GCA
Cuts at: 1707 1707
Size: 3926
HaeII r_GCGC'y
Cuts at: 564 743 807 1360 1608 2630 3609 564
Size: 179 64 553 248 1022 979 881
Fragments arranged by size:
1022 979 881 553 248 179 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1768 1798 1800 1830 1768
Size: 30 2 30 3864
Fragments arranged by size:
3864 30 30 2
HincII GTy'rAC
Cuts at: 965 965
Size: 3926
KasI G'GCGC_C
Cuts at: 739 1356 1604 739
Size: 617 248 3061
Fragments arranged by size:
3061 617 248
KpnI G_GTAC'C
Cuts at: 914 946 914
Size: 32 3894
MluI A'CGCG_T
Cuts at: 3359 3359
Size: 3926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 779 2597 2781 779
Size: 1818 184 1924
Fragments arranged by size:
1924 1818 184
MscI TGG'CCA
Cuts at: 163 210 491 1687 163
Size: 47 281 1196 2402
Fragments arranged by size:
2402 1196 281 47
MslI CAynn'nnrTG
Cuts at: 1915 2042 1915
Size: 127 3799
MspA1I CmG'CkG
Cuts at: 387 446 528 566 682 1037 1351 1711 2724
Size: 59 82 38 116 355 314 360 1013
Cuts at: 2724 2969 387
Size: 245 1344
Fragments arranged by size:
1344 1013 360 355 314 245 116 82
59 38
NaeI GCC'GGC
Cuts at: 2107 2107
Size: 3926
NarI GG'CG_CC
Cuts at: 740 1357 1605 740
Size: 617 248 3061
Fragments arranged by size:
3061 617 248
NcoI C'CATG_G
Cuts at: 242 242
Size: 3926
NgoMIV G'CCGG_C
Cuts at: 2105 2105
Size: 3926
NspI r_CATG'y
Cuts at: 979 1479 2386 979
Size: 500 907 2519
Fragments arranged by size:
2519 907 500
PasI CC'CwG_GG
Cuts at: 615 615
Size: 3926
PciI A'CATG_T
Cuts at: 2382 2382
Size: 3926
PflMI CCAn_nnn'nTGG
Cuts at: 390 390
Size: 3926
PmeI GTTT'AAAC
Cuts at: 925 925
Size: 3926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3065 3097 3065
Size: 32 3894
PpuMI rG'GwC_Cy
Cuts at: 450 450
Size: 3926
PspOMI G'GGCC_C
Cuts at: 299 299
Size: 3926
PstI C_TGCA'G
Cuts at: 527 973 1658 527
Size: 446 685 2795
Fragments arranged by size:
2795 685 446
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3926
PvuII CAG'CTG
Cuts at: 387 528 682 1351 1711 387
Size: 141 154 669 360 2602
Fragments arranged by size:
2602 669 360 154 141
RsrII CG'GwC_CG
Cuts at: 2121 2121
Size: 3926
SacI G_AGCT'C
Cuts at: 119 512 940 119
Size: 393 428 3105
Fragments arranged by size:
3105 428 393
SacII CC_GC'GG
Cuts at: 447 567 447
Size: 120 3806
SalI G'TCGA_C
Cuts at: 963 963
Size: 3926
SapI GCTCTTCn'nnn_
Cuts at: 517 1949 2159 517
Size: 1432 210 2284
Fragments arranged by size:
2284 1432 210
SbfI CC_TGCA'GG
Cuts at: 973 973
Size: 3926
ScaI AGT'ACT
Cuts at: 3443 3443
Size: 3926
SfcI C'TryA_G
Cuts at: 33 232 523 969 1654 2647 2838 33
Size: 199 291 446 685 993 191 1121
Fragments arranged by size:
1121 993 685 446 291 199 191
SfoI GGC'GCC
Cuts at: 741 1358 1606 741
Size: 617 248 3061
Fragments arranged by size:
3061 617 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3926
SmaI CCC'GGG
Cuts at: 578 948 578
Size: 370 3556
SmlI C'TyrA_G
Cuts at: 1081 1996 2488 2750 1081
Size: 915 492 262 2257
Fragments arranged by size:
2257 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3926
SphI G_CATG'C
Cuts at: 979 1479 979
Size: 500 3426
StyI C'CwwG_G
Cuts at: 242 627 914 1060 1414 242
Size: 385 287 146 354 2754
Fragments arranged by size:
2754 385 354 287 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1509 1642 2101 2310 1509
Size: 133 459 209 3125
Fragments arranged by size:
3125 459 209 133
TatI w'GTAC_w
Cuts at: 3441 3441
Size: 3926
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 719 751 3443 3475 719
Size: 32 2692 32 1170
Fragments arranged by size:
2692 1170 32 32
Tth111I GACn'n_nGTC
Cuts at: 1723 1723
Size: 3926
XbaI T'CTAG_A
Cuts at: 957 957
Size: 3926
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1421 1421
Size: 3926
XmaI C'CCGG_G
Cuts at: 576 946 576
Size: 370 3556
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I
BsaXI BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PfoI
PmlI PshAI PsiI PspXI PsrI SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI