(Circular) (Six-base) MAPSORT of: pF1KE2627.seq Check: 6756 from: 1 to: 3926 pF1KE2627 3926 bp With 182 enzymes: SgfI * May 31, 2016 13:40 .. AccI GT'mk_AC Cuts at: 964 964 Size: 3926 Acc65I G'GTAC_C Cuts at: 910 942 910 Size: 32 3894 AclI AA'CG_TT Cuts at: 3766 3766 Size: 3926 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 838 1751 2183 2930 3198 838 Size: 913 432 747 268 1566 Fragments arranged by size: 1566 913 747 432 268 AflIII A'CryG_T Cuts at: 1278 2382 3359 1278 Size: 1104 977 1845 Fragments arranged by size: 1845 1104 977 AgeI A'CCGG_T Cuts at: 2314 2314 Size: 3926 AlwNI CAG_nnn'CTG Cuts at: 219 282 390 900 1231 2798 219 Size: 63 108 510 331 1567 1347 Fragments arranged by size: 1567 1347 510 331 108 63 ApaI G_GGCC'C Cuts at: 303 303 Size: 3926 ApaLI G'TGCA_C Cuts at: 2696 2696 Size: 3926 ApoI r'AATT_y Cuts at: 697 930 3279 3535 697 Size: 233 2349 256 1088 Fragments arranged by size: 2349 1088 256 233 AseI AT'TA_AT Cuts at: 20 20 Size: 3926 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3926 AvaI C'yCGr_G Cuts at: 576 946 576 Size: 370 3556 BamHI G'GATC_C Cuts at: 632 951 632 Size: 319 3607 BanI G'GyrC_C Cuts at: 265 580 739 910 942 1356 1604 1639 265 Size: 315 159 171 32 414 248 35 2552 Fragments arranged by size: 2552 414 315 248 171 159 35 32 BanII G_rGCy'C Cuts at: 119 303 512 754 940 1970 119 Size: 184 209 242 186 1030 2075 Fragments arranged by size: 2075 1030 242 209 186 184 BbeI G_GCGC'C Cuts at: 743 1360 1608 743 Size: 617 248 3061 Fragments arranged by size: 3061 617 248 BbsI GAAGACnn'nnnn_ Cuts at: 171 708 171 Size: 537 3389 BciVI GTATCCnnnnn_n' Cuts at: 1824 2585 3926 1824 Size: 761 1341 1824 Fragments arranged by size: 1824 1341 761 BclI T'GATC_A Cuts at: 1446 1446 Size: 3926 BglI GCCn_nnn'nGGC Cuts at: 338 338 Size: 3926 BglII A'GATC_T Cuts at: 1441 3478 1441 Size: 2037 1889 BlpI GC'TnA_GC Cuts at: 1038 1038 Size: 3926 Bme1580I G_kGCm'C Cuts at: 303 583 1551 1644 2700 303 Size: 280 968 93 1056 1529 Fragments arranged by size: 1529 1056 968 280 93 BmgBI CAC'GTC Cuts at: 348 348 Size: 3926 BmrI ACTGGGnnnn_n' Cuts at: 720 1138 1219 1554 3698 720 Size: 418 81 335 2144 948 Fragments arranged by size: 2144 948 418 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 138 903 2293 138 Size: 765 1390 1771 Fragments arranged by size: 1771 1390 765 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 120 662 847 120 Size: 542 185 3199 Fragments arranged by size: 3199 542 185 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1102 1981 2473 2771 1102 Size: 879 492 298 2257 Fragments arranged by size: 2257 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3630 3630 Size: 3926 BsaAI yAC'GTr Cuts at: 375 771 1279 375 Size: 396 508 3022 Fragments arranged by size: 3022 508 396 BsaBI GATnn'nnATC Cuts at: 6 1440 3477 6 Size: 1434 2037 455 Fragments arranged by size: 2037 1434 455 BsaHI Gr'CG_yC Cuts at: 740 1357 1605 2307 3803 740 Size: 617 248 702 1496 863 Fragments arranged by size: 1496 863 702 617 248 BsaWI w'CCGG_w Cuts at: 1115 1636 2314 2588 2735 3146 1115 Size: 521 678 274 147 411 1895 Fragments arranged by size: 1895 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 160 748 862 160 Size: 588 114 3224 Fragments arranged by size: 3224 588 114 BseYI C'CCAG_C Cuts at: 383 390 1351 2109 2686 3182 383 Size: 7 961 758 577 496 1127 Fragments arranged by size: 1127 961 758 577 496 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 868 868 Size: 3926 BsiEI CG_ry'CG Cuts at: 104 1468 1514 2722 104 Size: 1364 46 1208 1308 Fragments arranged by size: 1364 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 119 512 536 940 1718 1908 2700 119 Size: 393 24 404 778 190 792 1345 Fragments arranged by size: 1345 792 778 404 393 190 24 BsmBI CGTCTCn'nnnn_ Cuts at: 354 354 Size: 3926 Bsp1286I G_dGCh'C Cuts at: 119 303 512 536 583 754 940 1551 1644 Size: 184 209 24 47 171 186 611 93 Cuts at: 1644 1718 1908 1970 2700 119 Size: 74 190 62 730 1345 Fragments arranged by size: 1345 730 611 209 190 186 184 171 93 74 62 47 24 BspEI T'CCGG_A Cuts at: 3146 3146 Size: 3926 BspHI T'CATG_A Cuts at: 3102 3102 Size: 3926 BspMI ACCTGCnnnn'nnnn_ Cuts at: 976 1190 1492 1873 976 Size: 214 302 381 3029 Fragments arranged by size: 3029 381 302 214 BsrBI CCG'CTC Cuts at: 50 122 412 668 2218 2272 3756 3921 50 Size: 72 290 256 1550 54 1484 165 55 Fragments arranged by size: 1550 1484 290 256 165 72 55 54 BsrDI GCAATG_nn' Cuts at: 1838 1838 Size: 3926 BsrFI r'CCGG_y Cuts at: 643 1924 2105 2314 643 Size: 1281 181 209 2255 Fragments arranged by size: 2255 1281 209 181 BssSI C'ACGA_G Cuts at: 2197 2555 2197 Size: 358 3568 BstAPI GCAn_nnn'nTGC Cuts at: 531 900 1303 531 Size: 369 403 3154 Fragments arranged by size: 3154 403 369 BstBI TT'CG_AA Cuts at: 2287 2287 Size: 3926 BstXI CCAn_nnnn'nTGG Cuts at: 628 628 Size: 3926 BstYI r'GATC_y Cuts at: 1 632 951 1418 1441 1776 2022 3034 3120 Size: 631 319 467 23 335 246 1012 86 Cuts at: 3120 3132 3478 1 Size: 12 346 449 Fragments arranged by size: 1012 631 467 449 346 335 319 246 86 23 12 BtgI C'CryG_G Cuts at: 242 444 564 904 242 Size: 202 120 340 3264 Fragments arranged by size: 3264 340 202 120 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1876 2057 2220 1876 Size: 181 163 3582 Fragments arranged by size: 3582 181 163 BtsI GCAGTG_nn' Cuts at: 520 818 1169 520 Size: 298 351 3277 Fragments arranged by size: 3277 351 298 DraI TTT'AAA Cuts at: 925 925 Size: 3926 DrdI GACnn_nn'nnGTC Cuts at: 457 712 1632 2490 457 Size: 255 920 858 1893 Fragments arranged by size: 1893 920 858 255 EaeI y'GGCC_r Cuts at: 161 208 401 489 1511 1685 2076 2103 3448 Size: 47 193 88 1022 174 391 27 1345 Cuts at: 3448 161 Size: 639 Fragments arranged by size: 1345 1022 639 391 193 174 88 47 27 EagI C'GGCC_G Cuts at: 1511 1511 Size: 3926 EarI CTCTTCn'nnn_ Cuts at: 178 517 1949 2159 178 Size: 339 1432 210 1945 Fragments arranged by size: 1945 1432 339 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 770 2134 2444 2590 3736 770 Size: 1364 310 146 1146 960 Fragments arranged by size: 1364 1146 960 310 146 EcoICRI GAG'CTC Cuts at: 117 510 938 117 Size: 393 428 3105 Fragments arranged by size: 3105 428 393 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 120 662 838 847 1751 2183 2930 3198 120 Size: 542 176 9 904 432 747 268 848 Fragments arranged by size: 904 848 747 542 432 268 176 9 EcoNI CCTnn'n_nnAGG Cuts at: 135 274 135 Size: 139 3787 EcoO109I rG'GnC_Cy Cuts at: 216 299 450 822 1065 216 Size: 83 151 372 243 3077 Fragments arranged by size: 3077 372 243 151 83 EcoRI G'AATT_C Cuts at: 930 930 Size: 3926 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1945 1945 Size: 3926 FspI TGC'GCA Cuts at: 1707 1707 Size: 3926 HaeII r_GCGC'y Cuts at: 564 743 807 1360 1608 2630 3609 564 Size: 179 64 553 248 1022 979 881 Fragments arranged by size: 1022 979 881 553 248 179 64 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1768 1798 1800 1830 1768 Size: 30 2 30 3864 Fragments arranged by size: 3864 30 30 2 HincII GTy'rAC Cuts at: 965 965 Size: 3926 KasI G'GCGC_C Cuts at: 739 1356 1604 739 Size: 617 248 3061 Fragments arranged by size: 3061 617 248 KpnI G_GTAC'C Cuts at: 914 946 914 Size: 32 3894 MluI A'CGCG_T Cuts at: 3359 3359 Size: 3926 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 779 2597 2781 779 Size: 1818 184 1924 Fragments arranged by size: 1924 1818 184 MscI TGG'CCA Cuts at: 163 210 491 1687 163 Size: 47 281 1196 2402 Fragments arranged by size: 2402 1196 281 47 MslI CAynn'nnrTG Cuts at: 1915 2042 1915 Size: 127 3799 MspA1I CmG'CkG Cuts at: 387 446 528 566 682 1037 1351 1711 2724 Size: 59 82 38 116 355 314 360 1013 Cuts at: 2724 2969 387 Size: 245 1344 Fragments arranged by size: 1344 1013 360 355 314 245 116 82 59 38 NaeI GCC'GGC Cuts at: 2107 2107 Size: 3926 NarI GG'CG_CC Cuts at: 740 1357 1605 740 Size: 617 248 3061 Fragments arranged by size: 3061 617 248 NcoI C'CATG_G Cuts at: 242 242 Size: 3926 NgoMIV G'CCGG_C Cuts at: 2105 2105 Size: 3926 NspI r_CATG'y Cuts at: 979 1479 2386 979 Size: 500 907 2519 Fragments arranged by size: 2519 907 500 PasI CC'CwG_GG Cuts at: 615 615 Size: 3926 PciI A'CATG_T Cuts at: 2382 2382 Size: 3926 PflMI CCAn_nnn'nTGG Cuts at: 390 390 Size: 3926 PmeI GTTT'AAAC Cuts at: 925 925 Size: 3926 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3065 3097 3065 Size: 32 3894 PpuMI rG'GwC_Cy Cuts at: 450 450 Size: 3926 PspOMI G'GGCC_C Cuts at: 299 299 Size: 3926 PstI C_TGCA'G Cuts at: 527 973 1658 527 Size: 446 685 2795 Fragments arranged by size: 2795 685 446 PvuI CG_AT'CG Cuts at: 104 104 Size: 3926 PvuII CAG'CTG Cuts at: 387 528 682 1351 1711 387 Size: 141 154 669 360 2602 Fragments arranged by size: 2602 669 360 154 141 RsrII CG'GwC_CG Cuts at: 2121 2121 Size: 3926 SacI G_AGCT'C Cuts at: 119 512 940 119 Size: 393 428 3105 Fragments arranged by size: 3105 428 393 SacII CC_GC'GG Cuts at: 447 567 447 Size: 120 3806 SalI G'TCGA_C Cuts at: 963 963 Size: 3926 SapI GCTCTTCn'nnn_ Cuts at: 517 1949 2159 517 Size: 1432 210 2284 Fragments arranged by size: 2284 1432 210 SbfI CC_TGCA'GG Cuts at: 973 973 Size: 3926 ScaI AGT'ACT Cuts at: 3443 3443 Size: 3926 SfcI C'TryA_G Cuts at: 33 232 523 969 1654 2647 2838 33 Size: 199 291 446 685 993 191 1121 Fragments arranged by size: 1121 993 685 446 291 199 191 SfoI GGC'GCC Cuts at: 741 1358 1606 741 Size: 617 248 3061 Fragments arranged by size: 3061 617 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3926 SmaI CCC'GGG Cuts at: 578 948 578 Size: 370 3556 SmlI C'TyrA_G Cuts at: 1081 1996 2488 2750 1081 Size: 915 492 262 2257 Fragments arranged by size: 2257 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3926 SphI G_CATG'C Cuts at: 979 1479 979 Size: 500 3426 StyI C'CwwG_G Cuts at: 242 627 914 1060 1414 242 Size: 385 287 146 354 2754 Fragments arranged by size: 2754 385 354 287 146 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1509 1642 2101 2310 1509 Size: 133 459 209 3125 Fragments arranged by size: 3125 459 209 133 TatI w'GTAC_w Cuts at: 3441 3441 Size: 3926 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 719 751 3443 3475 719 Size: 32 2692 32 1170 Fragments arranged by size: 2692 1170 32 32 Tth111I GACn'n_nGTC Cuts at: 1723 1723 Size: 3926 XbaI T'CTAG_A Cuts at: 957 957 Size: 3926 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1421 1421 Size: 3926 XmaI C'CCGG_G Cuts at: 576 946 576 Size: 370 3556 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI AscI AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsaXI BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PfoI PmlI PshAI PsiI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI