Restriction map for
pF1KE2635
(Circular) (Six-base) MAPSORT of: pF1KE2635.seq Check: 1397 from: 1 to: 4283
pF1KE2635 4283 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AccI GT'mk_AC
Cuts at: 1321 1321
Size: 4283
Acc65I G'GTAC_C
Cuts at: 1299 1299
Size: 4283
AclI AA'CG_TT
Cuts at: 4123 4123
Size: 4283
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 359 708 2108 2540 3287 3555 359
Size: 349 1400 432 747 268 1087
Fragments arranged by size:
1400 1087 747 432 349 268
AflII C'TTAA_G
Cuts at: 799 799
Size: 4283
AflIII A'CryG_T
Cuts at: 1635 2739 3716 1635
Size: 1104 977 2202
Fragments arranged by size:
2202 1104 977
AgeI A'CCGG_T
Cuts at: 2671 2671
Size: 4283
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 266 298 266
Size: 32 4251
AlwNI CAG_nnn'CTG
Cuts at: 1588 3155 1588
Size: 1567 2716
ApaLI G'TGCA_C
Cuts at: 3053 3053
Size: 4283
ApoI r'AATT_y
Cuts at: 167 301 344 421 1102 1287 3636 3892 167
Size: 134 43 77 681 185 2349 256 558
Fragments arranged by size:
2349 681 558 256 185 134 77 43
AseI AT'TA_AT
Cuts at: 20 20
Size: 4283
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4283
AvaI C'yCGr_G
Cuts at: 928 1303 928
Size: 375 3908
BamHI G'GATC_C
Cuts at: 1200 1308 1200
Size: 108 4175
BanI G'GyrC_C
Cuts at: 708 1299 1713 1961 1996 708
Size: 591 414 248 35 2995
Fragments arranged by size:
2995 591 414 248 35
BanII G_rGCy'C
Cuts at: 1297 2327 1297
Size: 1030 3253
BbeI G_GCGC'C
Cuts at: 1717 1965 1717
Size: 248 4035
BbsI GAAGACnn'nnnn_
Cuts at: 928 928
Size: 4283
BciVI GTATCCnnnnn_n'
Cuts at: 2181 2942 4283 2181
Size: 761 1341 2181
Fragments arranged by size:
2181 1341 761
BclI T'GATC_A
Cuts at: 1803 1803
Size: 4283
BglII A'GATC_T
Cuts at: 1798 3835 1798
Size: 2037 2246
BlpI GC'TnA_GC
Cuts at: 1395 1395
Size: 4283
Bme1580I G_kGCm'C
Cuts at: 1908 2001 3057 1908
Size: 93 1056 3134
Fragments arranged by size:
3134 1056 93
BmgBI CAC'GTC
Cuts at: 555 1002 555
Size: 447 3836
BmrI ACTGGGnnnn_n'
Cuts at: 1495 1576 1911 4055 1495
Size: 81 335 2144 1723
Fragments arranged by size:
2144 1723 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2650 2650
Size: 4283
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 293
Size: 4283
Bpu10I CC'TnA_GC
Cuts at: 623 913 623
Size: 290 3993
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 830 1226 1459 2338 2830 3128 830
Size: 396 233 879 492 298 1985
Fragments arranged by size:
1985 879 492 396 298 233
BsaI GGTCTCn'nnnn_
Cuts at: 3987 3987
Size: 4283
BsaAI yAC'GTr
Cuts at: 1636 1636
Size: 4283
BsaBI GATnn'nnATC
Cuts at: 6 1226 1797 3834 6
Size: 1220 571 2037 455
Fragments arranged by size:
2037 1220 571 455
BsaHI Gr'CG_yC
Cuts at: 1714 1962 2664 4160 1714
Size: 248 702 1496 1837
Fragments arranged by size:
1837 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1472 1993 2671 2945 3092 3503 1472
Size: 521 678 274 147 411 2252
Fragments arranged by size:
2252 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 266 296 266
Size: 30 4253
BseYI C'CCAG_C
Cuts at: 1708 2466 3043 3539 1708
Size: 758 577 496 2452
Fragments arranged by size:
2452 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1825 1871 3079 104
Size: 1721 46 1208 1308
Fragments arranged by size:
1721 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1297 2075 2265 3057 1297
Size: 778 190 792 2523
Fragments arranged by size:
2523 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1297 1908 2001 2075 2265 2327 3057 1297
Size: 611 93 74 190 62 730 2523
Fragments arranged by size:
2523 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3503 3503
Size: 4283
BspHI T'CATG_A
Cuts at: 632 3459 632
Size: 2827 1456
BspMI ACCTGCnnnn'nnnn_
Cuts at: 528 1333 1547 1849 2230 528
Size: 805 214 302 381 2581
Fragments arranged by size:
2581 805 381 302 214
BsrBI CCG'CTC
Cuts at: 50 933 2575 2629 4113 4278 50
Size: 883 1642 54 1484 165 55
Fragments arranged by size:
1642 1484 883 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1191 2195 1191
Size: 1004 3279
BsrFI r'CCGG_y
Cuts at: 2281 2462 2671 2281
Size: 181 209 3893
Fragments arranged by size:
3893 209 181
BssSI C'ACGA_G
Cuts at: 2554 2912 2554
Size: 358 3925
BstAPI GCAn_nnn'nTGC
Cuts at: 772 1660 772
Size: 888 3395
BstBI TT'CG_AA
Cuts at: 2644 2644
Size: 4283
BstEII G'GTnAC_C
Cuts at: 253 253
Size: 4283
BstYI r'GATC_y
Cuts at: 1 1200 1308 1775 1798 2133 2379 3391 3477
Size: 1199 108 467 23 335 246 1012 86
Cuts at: 3477 3489 3835 1
Size: 12 346 449
Fragments arranged by size:
1199 1012 467 449 346 335 246 108
86 23 12
BtgI C'CryG_G
Cuts at: 1052 1052
Size: 4283
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2233 2414 2577 2233
Size: 181 163 3939
Fragments arranged by size:
3939 181 163
BtsI GCAGTG_nn'
Cuts at: 770 1526 770
Size: 756 3527
DraI TTT'AAA
Cuts at: 426 1282 426
Size: 856 3427
DrdI GACnn_nn'nnGTC
Cuts at: 1989 2847 1989
Size: 858 3425
EaeI y'GGCC_r
Cuts at: 1055 1868 2042 2433 2460 3805 1055
Size: 813 174 391 27 1345 1533
Fragments arranged by size:
1533 1345 813 391 174 27
EagI C'GGCC_G
Cuts at: 1868 1868
Size: 4283
EarI CTCTTCn'nnn_
Cuts at: 505 2306 2516 505
Size: 1801 210 2272
Fragments arranged by size:
2272 1801 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 109 778 2491 2801 2947 4093 109
Size: 669 1713 310 146 1146 299
Fragments arranged by size:
1713 1146 669 310 299 146
EcoICRI GAG'CTC
Cuts at: 1295 1295
Size: 4283
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 359 708 2108 2540 3287 3555 293
Size: 66 349 1400 432 747 268 1021
Fragments arranged by size:
1400 1021 747 432 349 268 66
EcoO109I rG'GnC_Cy
Cuts at: 547 627 1422 547
Size: 80 795 3408
Fragments arranged by size:
3408 795 80
EcoRI G'AATT_C
Cuts at: 167 1287 167
Size: 1120 3163
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2302 2302
Size: 4283
FspI TGC'GCA
Cuts at: 2064 2064
Size: 4283
HaeII r_GCGC'y
Cuts at: 598 1717 1965 2987 3966 598
Size: 1119 248 1022 979 915
Fragments arranged by size:
1119 1022 979 915 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2125 2155 2157 2187 2125
Size: 30 2 30 4221
Fragments arranged by size:
4221 30 30 2
HincII GTy'rAC
Cuts at: 1322 1322
Size: 4283
KasI G'GCGC_C
Cuts at: 1713 1961 1713
Size: 248 4035
KpnI G_GTAC'C
Cuts at: 1303 1303
Size: 4283
MluI A'CGCG_T
Cuts at: 3716 3716
Size: 4283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2954 3138 2954
Size: 184 4099
MscI TGG'CCA
Cuts at: 1057 2044 1057
Size: 987 3296
MslI CAynn'nnrTG
Cuts at: 589 1134 2272 2399 589
Size: 545 1138 127 2473
Fragments arranged by size:
2473 1138 545 127
MspA1I CmG'CkG
Cuts at: 1394 1708 2068 3081 3326 1394
Size: 314 360 1013 245 2351
Fragments arranged by size:
2351 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2464 2464
Size: 4283
NarI GG'CG_CC
Cuts at: 1714 1962 1714
Size: 248 4035
NcoI C'CATG_G
Cuts at: 1052 1052
Size: 4283
NgoMIV G'CCGG_C
Cuts at: 2462 2462
Size: 4283
NspI r_CATG'y
Cuts at: 1336 1836 2743 1336
Size: 500 907 2876
Fragments arranged by size:
2876 907 500
PciI A'CATG_T
Cuts at: 2739 2739
Size: 4283
PfoI T'CCnGG_A
Cuts at: 245 245
Size: 4283
PmeI GTTT'AAAC
Cuts at: 1282 1282
Size: 4283
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 266 298 3422 3454 266
Size: 32 3124 32 1095
Fragments arranged by size:
3124 1095 32 32
PshAI GACnn'nnGTC
Cuts at: 993 993
Size: 4283
PspXI vC'TCGA_Gb
Cuts at: 928 928
Size: 4283
PstI C_TGCA'G
Cuts at: 459 1330 2015 459
Size: 871 685 2727
Fragments arranged by size:
2727 871 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4283
PvuII CAG'CTG
Cuts at: 1708 2068 1708
Size: 360 3923
RsrII CG'GwC_CG
Cuts at: 2478 2478
Size: 4283
SacI G_AGCT'C
Cuts at: 1297 1297
Size: 4283
SalI G'TCGA_C
Cuts at: 1320 1320
Size: 4283
SapI GCTCTTCn'nnn_
Cuts at: 2306 2516 2306
Size: 210 4073
SbfI CC_TGCA'GG
Cuts at: 1330 1330
Size: 4283
ScaI AGT'ACT
Cuts at: 3800 3800
Size: 4283
SfcI C'TryA_G
Cuts at: 33 455 698 1326 2011 3004 3195 33
Size: 422 243 628 685 993 191 1121
Fragments arranged by size:
1121 993 685 628 422 243 191
SfoI GGC'GCC
Cuts at: 1715 1963 1715
Size: 248 4035
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4283
SmaI CCC'GGG
Cuts at: 1305 1305
Size: 4283
SmlI C'TyrA_G
Cuts at: 799 845 928 1241 1438 2353 2845 3107 799
Size: 46 83 313 197 915 492 262 1975
Fragments arranged by size:
1975 915 492 313 262 197 83 46
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4283
SphI G_CATG'C
Cuts at: 1336 1836 1336
Size: 500 3783
StyI C'CwwG_G
Cuts at: 1043 1052 1417 1771 1043
Size: 9 365 354 3555
Fragments arranged by size:
3555 365 354 9
SwaI ATTT'AAAT
Cuts at: 426 426
Size: 4283
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 272 1242 1866 1999 2458 2667 272
Size: 970 624 133 459 209 1888
Fragments arranged by size:
1888 970 624 459 209 133
TatI w'GTAC_w
Cuts at: 364 561 3798 364
Size: 197 3237 849
Fragments arranged by size:
3237 849 197
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 224 256 424 456 3800 3832 224
Size: 32 168 32 3344 32 675
Fragments arranged by size:
3344 675 168 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2080 2080
Size: 4283
XbaI T'CTAG_A
Cuts at: 1314 1314
Size: 4283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1050 1092 1778 1050
Size: 42 686 3555
Fragments arranged by size:
3555 686 42
XhoI C'TCGA_G
Cuts at: 928 928
Size: 4283
XmaI C'CCGG_G
Cuts at: 1303 1303
Size: 4283
XmnI GAAnn'nnTTC
Cuts at: 718 718
Size: 4283
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PshAI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmtI BseRI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PpuMI PsiI PspOMI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI ZraI