Restriction map for
pF1KE2637
(Circular) (Six-base) MAPSORT of: pF1KE2637.seq Check: 7971 from: 1 to: 5387
pF1KE2637 5387 bp
With 182 enzymes: SgfI *
February 19, 2021 20:23 ..
AatII G_ACGT'C
Cuts at: 779 779
Size: 5387
AccI GT'mk_AC
Cuts at: 1985 2318 2425 1985
Size: 333 107 4947
Fragments arranged by size:
4947 333 107
Acc65I G'GTAC_C
Cuts at: 2403 2403
Size: 5387
AclI AA'CG_TT
Cuts at: 5227 5227
Size: 5387
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 736 1837 1876 3212 3644 4391 4659 437
Size: 299 1101 39 1336 432 747 268 1165
Fragments arranged by size:
1336 1165 1101 747 432 299 268 39
AflIII A'CryG_T
Cuts at: 1297 1953 2264 2739 3843 4820 1297
Size: 656 311 475 1104 977 1864
Fragments arranged by size:
1864 1104 977 656 475 311
AgeI A'CCGG_T
Cuts at: 3775 3775
Size: 5387
AhdI GACnn_n'nnGTC
Cuts at: 2352 2352
Size: 5387
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1202 1202
Size: 5387
AlwNI CAG_nnn'CTG
Cuts at: 631 2692 4259 631
Size: 2061 1567 1759
Fragments arranged by size:
2061 1759 1567
ApaI G_GGCC'C
Cuts at: 595 968 1098 1126 595
Size: 373 130 28 4856
Fragments arranged by size:
4856 373 130 28
ApaLI G'TGCA_C
Cuts at: 4157 4157
Size: 5387
ApoI r'AATT_y
Cuts at: 2391 4740 4996 2391
Size: 2349 256 2782
Fragments arranged by size:
2782 2349 256
AseI AT'TA_AT
Cuts at: 20 1892 20
Size: 1872 3515
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5387
AvaI C'yCGr_G
Cuts at: 1118 1718 1959 2407 1118
Size: 600 241 448 4098
Fragments arranged by size:
4098 600 448 241
BamHI G'GATC_C
Cuts at: 2412 2412
Size: 5387
BanI G'GyrC_C
Cuts at: 895 2403 2817 3065 3100 895
Size: 1508 414 248 35 3182
Fragments arranged by size:
3182 1508 414 248 35
BanII G_rGCy'C
Cuts at: 119 289 360 576 595 959 968 1098 1126
Size: 170 71 216 19 364 9 130 28
Cuts at: 1126 1169 1727 2401 3431 119
Size: 43 558 674 1030 2075
Fragments arranged by size:
2075 1030 674 558 364 216 170 130
71 43 28 19 9
BbeI G_GCGC'C
Cuts at: 2821 3069 2821
Size: 248 5139
BbsI GAAGACnn'nnnn_
Cuts at: 458 773 1760 458
Size: 315 987 4085
Fragments arranged by size:
4085 987 315
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2338 2372 2338
Size: 34 5353
BciVI GTATCCnnnnn_n'
Cuts at: 3285 4046 5387 3285
Size: 761 1341 3285
Fragments arranged by size:
3285 1341 761
BclI T'GATC_A
Cuts at: 2907 2907
Size: 5387
BglI GCCn_nnn'nGGC
Cuts at: 963 1547 2365 963
Size: 584 818 3985
Fragments arranged by size:
3985 818 584
BglII A'GATC_T
Cuts at: 2902 4939 2902
Size: 2037 3350
BlpI GC'TnA_GC
Cuts at: 113 2499 113
Size: 2386 3001
Bme1580I G_kGCm'C
Cuts at: 595 968 1098 1126 1405 1805 3012 3105 4161
Size: 373 130 28 279 400 1207 93 1056
Cuts at: 4161 595
Size: 1821
Fragments arranged by size:
1821 1207 1056 400 373 279 130 93
28
BmgBI CAC'GTC
Cuts at: 1956 2145 2267 1956
Size: 189 122 5076
Fragments arranged by size:
5076 189 122
BmrI ACTGGGnnnn_n'
Cuts at: 962 2599 2680 3015 5159 962
Size: 1637 81 335 2144 1190
Fragments arranged by size:
2144 1637 1190 335 81
BmtI G_CTAG'C
Cuts at: 2344 2344
Size: 5387
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3754 3754
Size: 5387
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1024 1121 1371 1484 1520 1024
Size: 97 250 113 36 4891
Fragments arranged by size:
4891 250 113 97 36
Bpu10I CC'TnA_GC
Cuts at: 662 1336 2002 662
Size: 674 666 4047
Fragments arranged by size:
4047 674 666
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 1468 2563 3442 3934 4232 655
Size: 813 1095 879 492 298 1810
Fragments arranged by size:
1810 1095 879 813 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 683 5091 683
Size: 4408 979
BsaAI yAC'GTr
Cuts at: 471 1020 2740 471
Size: 549 1720 3118
Fragments arranged by size:
3118 1720 549
BsaBI GATnn'nnATC
Cuts at: 6 2901 4938 6
Size: 2895 2037 455
Fragments arranged by size:
2895 2037 455
BsaHI Gr'CG_yC
Cuts at: 776 2818 3066 3768 5264 776
Size: 2042 248 702 1496 899
Fragments arranged by size:
2042 1496 899 702 248
BsaWI w'CCGG_w
Cuts at: 2576 3097 3775 4049 4196 4607 2576
Size: 521 678 274 147 411 3356
Fragments arranged by size:
3356 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 997 1027 2221 2251 997
Size: 30 1194 30 4133
Fragments arranged by size:
4133 1194 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 769 1078 2283 769
Size: 309 1205 3873
Fragments arranged by size:
3873 1205 309
BseYI C'CCAG_C
Cuts at: 151 442 822 885 945 1606 2327 2812 3570
Size: 291 380 63 60 661 721 485 758
Cuts at: 3570 4147 4643 151
Size: 577 496 895
Fragments arranged by size:
895 758 721 661 577 496 485 380
291 63 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 679 1060 1903 679
Size: 381 843 4163
Fragments arranged by size:
4163 843 381
BsiEI CG_ry'CG
Cuts at: 104 651 2929 2975 4183 104
Size: 547 2278 46 1208 1308
Fragments arranged by size:
2278 1308 1208 547 46
BsiHKAI G_wGCw'C
Cuts at: 313 360 2401 3179 3369 4161 313
Size: 47 2041 778 190 792 1539
Fragments arranged by size:
2041 1539 792 778 190 47
BsiWI C'GTAC_G
Cuts at: 158 158
Size: 5387
BsmBI CGTCTCn'nnnn_
Cuts at: 1437 2273 1437
Size: 836 4551
Bsp1286I G_dGCh'C
Cuts at: 119 289 313 360 576 595 959 968 1098
Size: 170 24 47 216 19 364 9 130
Cuts at: 1098 1126 1169 1405 1727 1805 2401 3012 3105
Size: 28 43 236 322 78 596 611 93
Cuts at: 3105 3179 3369 3431 4161 119
Size: 74 190 62 730 1345
Fragments arranged by size:
1345 730 611 596 364 322 236 216
190 170 130 93 78 74 62 47
43 28 24 19 9
BspEI T'CCGG_A
Cuts at: 4607 4607
Size: 5387
BspHI T'CATG_A
Cuts at: 4563 4563
Size: 5387
BspMI ACCTGCnnnn'nnnn_
Cuts at: 701 2437 2651 2953 3334 701
Size: 1736 214 302 381 2754
Fragments arranged by size:
2754 1736 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1591 1685 3679 3733 5217 5382 50
Size: 1541 94 1994 54 1484 165 55
Fragments arranged by size:
1994 1541 1484 165 94 55 54
BsrDI GCAATG_nn'
Cuts at: 2292 3299 2292
Size: 1007 4380
BsrFI r'CCGG_y
Cuts at: 2299 3385 3566 3775 2299
Size: 1086 181 209 3911
Fragments arranged by size:
3911 1086 209 181
BsrGI T'GTAC_A
Cuts at: 2237 2237
Size: 5387
BssSI C'ACGA_G
Cuts at: 3658 4016 3658
Size: 358 5029
BstAPI GCAn_nnn'nTGC
Cuts at: 2764 2764
Size: 5387
BstBI TT'CG_AA
Cuts at: 131 3748 131
Size: 3617 1770
BstEII G'GTnAC_C
Cuts at: 2010 2010
Size: 5387
BstXI CCAn_nnnn'nTGG
Cuts at: 1734 2149 1734
Size: 415 4972
BstYI r'GATC_y
Cuts at: 1 271 395 941 1905 2412 2879 2902 3237
Size: 270 124 546 964 507 467 23 335
Cuts at: 3237 3483 4495 4581 4593 4939 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 964 546 507 467 449 346 335
270 246 124 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 587 632 587
Size: 45 5342
BtgI C'CryG_G
Cuts at: 107 1195 1519 2220 2272 107
Size: 1088 324 701 52 3222
Fragments arranged by size:
3222 1088 701 324 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1286 3337 3518 3681 1286
Size: 2051 181 163 2992
Fragments arranged by size:
2992 2051 181 163
BtsI GCAGTG_nn'
Cuts at: 1745 1977 2630 1745
Size: 232 653 4502
Fragments arranged by size:
4502 653 232
DraI TTT'AAA
Cuts at: 2386 2386
Size: 5387
DrdI GACnn_nn'nnGTC
Cuts at: 775 3093 3951 775
Size: 2318 858 2211
Fragments arranged by size:
2318 2211 858
EaeI y'GGCC_r
Cuts at: 648 2061 2366 2972 3146 3537 3564 4909 648
Size: 1413 305 606 174 391 27 1345 1126
Fragments arranged by size:
1413 1345 1126 606 391 305 174 27
EagI C'GGCC_G
Cuts at: 648 2972 648
Size: 2324 3063
EarI CTCTTCn'nnn_
Cuts at: 1440 3410 3620 1440
Size: 1970 210 3207
Fragments arranged by size:
3207 1970 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1761 2168 2242 3595 3905 4051 5197 1761
Size: 407 74 1353 310 146 1146 1951
Fragments arranged by size:
1951 1353 1146 407 310 146 74
EcoICRI GAG'CTC
Cuts at: 358 2399 358
Size: 2041 3346
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 736 1024 1121 1371 1484 1520 1837 1876
Size: 299 288 97 250 113 36 317 39
Cuts at: 1876 3212 3644 4391 4659 437
Size: 1336 432 747 268 1165
Fragments arranged by size:
1336 1165 747 432 317 299 288 268
250 113 97 39 36
EcoNI CCTnn'n_nnAGG
Cuts at: 586 697 2005 2153 586
Size: 111 1308 148 3820
Fragments arranged by size:
3820 1308 148 111
EcoO109I rG'GnC_Cy
Cuts at: 529 591 592 628 1094 1095 1122 1123 1218
Size: 62 1 36 466 1 27 1 95
Cuts at: 1218 2123 2526 529
Size: 905 403 3390
Fragments arranged by size:
3390 905 466 403 95 62 36 27
1 1 1
EcoRI G'AATT_C
Cuts at: 2391 2391
Size: 5387
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2109 2205 3406 2109
Size: 96 1201 4090
Fragments arranged by size:
4090 1201 96
FspI TGC'GCA
Cuts at: 3168 3168
Size: 5387
HaeII r_GCGC'y
Cuts at: 2821 3069 4091 5070 2821
Size: 248 1022 979 3138
Fragments arranged by size:
3138 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 129 161 256 288 772 804 907 939 2338
Size: 32 95 32 484 32 103 32 1399
Cuts at: 2338 2370 3229 3259 3261 3291 129
Size: 32 859 30 2 30 2225
Fragments arranged by size:
2225 1399 859 484 103 95 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1998 2426 1998
Size: 428 4959
HindIII A'AGCT_T
Cuts at: 2086 2086
Size: 5387
KasI G'GCGC_C
Cuts at: 2817 3065 2817
Size: 248 5139
KpnI G_GTAC'C
Cuts at: 2407 2407
Size: 5387
MluI A'CGCG_T
Cuts at: 4820 4820
Size: 5387
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 340 1404 1729 4058 4242 340
Size: 1064 325 2329 184 1485
Fragments arranged by size:
2329 1485 1064 325 184
MscI TGG'CCA
Cuts at: 2063 2368 3148 2063
Size: 305 780 4302
Fragments arranged by size:
4302 780 305
MslI CAynn'nnrTG
Cuts at: 2311 3376 3503 2311
Size: 1065 127 4195
Fragments arranged by size:
4195 1065 127
MspA1I CmG'CkG
Cuts at: 322 415 714 889 1197 1521 1643 2498 2812
Size: 93 299 175 308 324 122 855 314
Cuts at: 2812 3172 4185 4430 322
Size: 360 1013 245 1279
Fragments arranged by size:
1279 1013 855 360 324 314 308 299
245 175 122 93
NaeI GCC'GGC
Cuts at: 3568 3568
Size: 5387
NarI GG'CG_CC
Cuts at: 2818 3066 2818
Size: 248 5139
NcoI C'CATG_G
Cuts at: 107 107
Size: 5387
NgoMIV G'CCGG_C
Cuts at: 3566 3566
Size: 5387
NheI G'CTAG_C
Cuts at: 2340 2340
Size: 5387
NotI GC'GGCC_GC
Cuts at: 648 648
Size: 5387
NspI r_CATG'y
Cuts at: 567 2440 2940 3847 567
Size: 1873 500 907 2107
Fragments arranged by size:
2107 1873 907 500
PciI A'CATG_T
Cuts at: 3843 3843
Size: 5387
PflMI CCAn_nnn'nTGG
Cuts at: 2059 2059
Size: 5387
PfoI T'CCnGG_A
Cuts at: 503 503
Size: 5387
PmeI GTTT'AAAC
Cuts at: 2386 2386
Size: 5387
PmlI CAC'GTG
Cuts at: 471 1020 471
Size: 549 4838
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4526 4558 4526
Size: 32 5355
PpuMI rG'GwC_Cy
Cuts at: 628 1218 2123 628
Size: 590 905 3892
Fragments arranged by size:
3892 905 590
PspOMI G'GGCC_C
Cuts at: 591 964 1094 1122 591
Size: 373 130 28 4856
Fragments arranged by size:
4856 373 130 28
PstI C_TGCA'G
Cuts at: 254 1790 2434 3119 254
Size: 1536 644 685 2522
Fragments arranged by size:
2522 1536 685 644
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5387
PvuII CAG'CTG
Cuts at: 415 2812 3172 415
Size: 2397 360 2630
Fragments arranged by size:
2630 2397 360
RsrII CG'GwC_CG
Cuts at: 3582 3582
Size: 5387
SacI G_AGCT'C
Cuts at: 360 2401 360
Size: 2041 3346
SacII CC_GC'GG
Cuts at: 1198 1522 1198
Size: 324 5063
SalI G'TCGA_C
Cuts at: 2424 2424
Size: 5387
SanDI GG'GwC_CC
Cuts at: 1218 2123 1218
Size: 905 4482
SapI GCTCTTCn'nnn_
Cuts at: 3410 3620 3410
Size: 210 5177
SbfI CC_TGCA'GG
Cuts at: 2434 2434
Size: 5387
ScaI AGT'ACT
Cuts at: 4904 4904
Size: 5387
SfcI C'TryA_G
Cuts at: 33 250 1647 1786 2430 3115 4108 4299 33
Size: 217 1397 139 644 685 993 191 1121
Fragments arranged by size:
1397 1121 993 685 644 217 191 139
SfiI GGCCn_nnn'nGGCC
Cuts at: 1547 1547
Size: 5387
SfoI GGC'GCC
Cuts at: 2819 3067 2819
Size: 248 5139
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5387
SmaI CCC'GGG
Cuts at: 1120 1720 1961 2409 1120
Size: 600 241 448 4098
Fragments arranged by size:
4098 600 448 241
SmlI C'TyrA_G
Cuts at: 670 1483 2542 3457 3949 4211 670
Size: 813 1059 915 492 262 1846
Fragments arranged by size:
1846 1059 915 813 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5387
SphI G_CATG'C
Cuts at: 567 2440 2940 567
Size: 1873 500 3014
Fragments arranged by size:
3014 1873 500
StuI AGG'CCT
Cuts at: 817 1329 817
Size: 512 4875
StyI C'CwwG_G
Cuts at: 107 812 2521 2875 107
Size: 705 1709 354 2619
Fragments arranged by size:
2619 1709 705 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1047 1620 1728 1770 2155 2970 3103 3562 3771
Size: 573 108 42 385 815 133 459 209
Cuts at: 3771 1047
Size: 2663
Fragments arranged by size:
2663 815 573 459 385 209 133 108
42
TatI w'GTAC_w
Cuts at: 2237 4902 2237
Size: 2665 2722
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1554 1586 4904 4936 1554
Size: 32 3318 32 2005
Fragments arranged by size:
3318 2005 32 32
Tth111I GACn'n_nGTC
Cuts at: 2104 3184 2104
Size: 1080 4307
XbaI T'CTAG_A
Cuts at: 2418 2418
Size: 5387
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1927 2116 2376 2882 1927
Size: 189 260 506 4432
Fragments arranged by size:
4432 506 260 189
XmaI C'CCGG_G
Cuts at: 1118 1718 1959 2407 1118
Size: 600 241 448 4098
Fragments arranged by size:
4098 600 448 241
ZraI GAC'GTC
Cuts at: 777 777
Size: 5387
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NotI
NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AloI AscI AvrII BaeI
BbvCI BfrBI BsmI BssHII BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HpaI MfeI NdeI NruI NsiI
PacI PasI PshAI PsiI PspXI PsrI SexAI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI