Restriction map for
pF1KE2640
(Circular) (Six-base) MAPSORT of: pF1KE2640.seq Check: 3928 from: 1 to: 4295
pF1KE2640 4295 bp
With 182 enzymes: SgfI *
October 5, 2016 15:26 ..
AccI GT'mk_AC
Cuts at: 785 1004 1333 785
Size: 219 329 3747
Fragments arranged by size:
3747 329 219
Acc65I G'GTAC_C
Cuts at: 518 1311 518
Size: 793 3502
AclI AA'CG_TT
Cuts at: 4135 4135
Size: 4295
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 759 2120 2552 3299 3567 759
Size: 1361 432 747 268 1487
Fragments arranged by size:
1487 1361 747 432 268
AflIII A'CryG_T
Cuts at: 566 1118 1647 2751 3728 566
Size: 552 529 1104 977 1133
Fragments arranged by size:
1133 1104 977 552 529
AgeI A'CCGG_T
Cuts at: 2683 2683
Size: 4295
AleI CACnn'nnGTG
Cuts at: 511 1123 511
Size: 612 3683
AlwNI CAG_nnn'CTG
Cuts at: 228 249 489 747 1600 3167 228
Size: 21 240 258 853 1567 1356
Fragments arranged by size:
1567 1356 853 258 240 21
ApaLI G'TGCA_C
Cuts at: 147 3065 147
Size: 2918 1377
ApoI r'AATT_y
Cuts at: 1299 3648 3904 1299
Size: 2349 256 1690
Fragments arranged by size:
2349 1690 256
AscI GG'CGCG_CC
Cuts at: 558 558
Size: 4295
AseI AT'TA_AT
Cuts at: 20 20
Size: 4295
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4295
AvaI C'yCGr_G
Cuts at: 205 1315 205
Size: 1110 3185
BamHI G'GATC_C
Cuts at: 1320 1320
Size: 4295
BanI G'GyrC_C
Cuts at: 172 405 518 628 814 1203 1311 1725 1973
Size: 233 113 110 186 389 108 414 248
Cuts at: 1973 2008 172
Size: 35 2459
Fragments arranged by size:
2459 414 389 248 233 186 113 110
108 35
BanII G_rGCy'C
Cuts at: 206 1285 1309 2339 206
Size: 1079 24 1030 2162
Fragments arranged by size:
2162 1079 1030 24
BbeI G_GCGC'C
Cuts at: 176 632 818 1207 1729 1977 176
Size: 456 186 389 522 248 2494
Fragments arranged by size:
2494 522 456 389 248 186
BbsI GAAGACnn'nnnn_
Cuts at: 174 174
Size: 4295
BbvCI CC'TCA_GC
Cuts at: 268 268
Size: 4295
BciVI GTATCCnnnnn_n'
Cuts at: 2193 2954 4295 2193
Size: 761 1341 2193
Fragments arranged by size:
2193 1341 761
BclI T'GATC_A
Cuts at: 1088 1815 1088
Size: 727 3568
BglI GCCn_nnn'nGGC
Cuts at: 278 278
Size: 4295
BglII A'GATC_T
Cuts at: 1810 3847 1810
Size: 2037 2258
BlpI GC'TnA_GC
Cuts at: 1407 1407
Size: 4295
Bme1580I G_kGCm'C
Cuts at: 151 401 1920 2013 3069 151
Size: 250 1519 93 1056 1377
Fragments arranged by size:
1519 1377 1056 250 93
BmgBI CAC'GTC
Cuts at: 567 1112 567
Size: 545 3750
BmrI ACTGGGnnnn_n'
Cuts at: 1507 1588 1923 4067 1507
Size: 81 335 2144 1735
Fragments arranged by size:
2144 1735 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2662 2662
Size: 4295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 250 313 214
Size: 36 63 4196
Fragments arranged by size:
4196 63 36
Bpu10I CC'TnA_GC
Cuts at: 268 268
Size: 4295
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 1471 2350 2842 3140 298
Size: 1173 879 492 298 1453
Fragments arranged by size:
1453 1173 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3999 3999
Size: 4295
BsaAI yAC'GTr
Cuts at: 723 768 1121 1648 723
Size: 45 353 527 3370
Fragments arranged by size:
3370 527 353 45
BsaBI GATnn'nnATC
Cuts at: 6 1809 3846 6
Size: 1803 2037 455
Fragments arranged by size:
2037 1803 455
BsaHI Gr'CG_yC
Cuts at: 173 629 815 878 1204 1726 1974 2676 4172
Size: 456 186 63 326 522 248 702 1496
Cuts at: 4172 173
Size: 296
Fragments arranged by size:
1496 702 522 456 326 296 248 186
63
BsaWI w'CCGG_w
Cuts at: 1484 2005 2683 2957 3104 3515 1484
Size: 521 678 274 147 411 2264
Fragments arranged by size:
2264 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 301 301
Size: 4295
BseYI C'CCAG_C
Cuts at: 409 453 546 1720 2478 3055 3551 409
Size: 44 93 1174 758 577 496 1153
Fragments arranged by size:
1174 1153 758 577 496 93 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 511 1003 1215 1225 355
Size: 156 492 212 10 3425
Fragments arranged by size:
3425 492 212 156 10
BsiEI CG_ry'CG
Cuts at: 104 1161 1837 1883 3091 104
Size: 1057 676 46 1208 1308
Fragments arranged by size:
1308 1208 1057 676 46
BsiHKAI G_wGCw'C
Cuts at: 151 590 656 887 1039 1285 1309 2087 2277
Size: 439 66 231 152 246 24 778 190
Cuts at: 2277 3069 151
Size: 792 1377
Fragments arranged by size:
1377 792 778 439 246 231 190 152
66 24
Bsp1286I G_dGCh'C
Cuts at: 151 206 401 590 656 887 1039 1285 1309
Size: 55 195 189 66 231 152 246 24
Cuts at: 1309 1920 2013 2087 2277 2339 3069 151
Size: 611 93 74 190 62 730 1377
Fragments arranged by size:
1377 730 611 246 231 195 190 189
152 93 74 66 62 55 24
BspEI T'CCGG_A
Cuts at: 3515 3515
Size: 4295
BspHI T'CATG_A
Cuts at: 845 1268 3471 845
Size: 423 2203 1669
Fragments arranged by size:
2203 1669 423
BspMI ACCTGCnnnn'nnnn_
Cuts at: 170 530 1345 1559 1861 2242 170
Size: 360 815 214 302 381 2223
Fragments arranged by size:
2223 815 381 360 302 214
BsrBI CCG'CTC
Cuts at: 50 219 465 2587 2641 4125 4290 50
Size: 169 246 2122 54 1484 165 55
Fragments arranged by size:
2122 1484 246 169 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2207 2207
Size: 4295
BsrFI r'CCGG_y
Cuts at: 328 349 2293 2474 2683 328
Size: 21 1944 181 209 1940
Fragments arranged by size:
1944 1940 209 181 21
BssHII G'CGCG_C
Cuts at: 256 558 890 941 256
Size: 302 332 51 3610
Fragments arranged by size:
3610 332 302 51
BssSI C'ACGA_G
Cuts at: 603 829 1038 2566 2924 603
Size: 226 209 1528 358 1974
Fragments arranged by size:
1974 1528 358 226 209
BstAPI GCAn_nnn'nTGC
Cuts at: 123 249 620 1672 123
Size: 126 371 1052 2746
Fragments arranged by size:
2746 1052 371 126
BstBI TT'CG_AA
Cuts at: 2656 2656
Size: 4295
BstYI r'GATC_y
Cuts at: 1 1320 1787 1810 2145 2391 3403 3489 3501
Size: 1319 467 23 335 246 1012 86 12
Cuts at: 3501 3847 1
Size: 346 449
Fragments arranged by size:
1319 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 176 572 968 1096 1232 1260 1286 176
Size: 396 396 128 136 28 26 3185
Fragments arranged by size:
3185 396 396 136 128 28 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2245 2426 2589 2245
Size: 181 163 3951
Fragments arranged by size:
3951 181 163
BtsI GCAGTG_nn'
Cuts at: 343 1538 343
Size: 1195 3100
DraI TTT'AAA
Cuts at: 1294 1294
Size: 4295
DraIII CAC_nnn'GTG
Cuts at: 620 620
Size: 4295
DrdI GACnn_nn'nnGTC
Cuts at: 2001 2859 2001
Size: 858 3437
EaeI y'GGCC_r
Cuts at: 575 1158 1880 2054 2445 2472 3817 575
Size: 583 722 174 391 27 1345 1053
Fragments arranged by size:
1345 1053 722 583 391 174 27
EagI C'GGCC_G
Cuts at: 1158 1880 1158
Size: 722 3573
EarI CTCTTCn'nnn_
Cuts at: 2318 2528 2318
Size: 210 4085
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 941 1203 2503 2813 2959 4105 941
Size: 262 1300 310 146 1146 1131
Fragments arranged by size:
1300 1146 1131 310 262 146
EcoICRI GAG'CTC
Cuts at: 1283 1307 1283
Size: 24 4271
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 250 313 759 2120 2552 3299 3567 214
Size: 36 63 446 1361 432 747 268 942
Fragments arranged by size:
1361 942 747 446 432 268 63 36
EcoNI CCTnn'n_nnAGG
Cuts at: 271 513 271
Size: 242 4053
EcoO109I rG'GnC_Cy
Cuts at: 744 1101 1434 744
Size: 357 333 3605
Fragments arranged by size:
3605 357 333
EcoRI G'AATT_C
Cuts at: 1299 1299
Size: 4295
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2314 2314
Size: 4295
FspI TGC'GCA
Cuts at: 127 2076 127
Size: 1949 2346
FspAI rTGC'GCAy
Cuts at: 127 127
Size: 4295
HaeII r_GCGC'y
Cuts at: 139 176 345 632 730 818 1207 1729 1977
Size: 37 169 287 98 88 389 522 248
Cuts at: 1977 2999 3978 139
Size: 1022 979 456
Fragments arranged by size:
1022 979 522 456 389 287 248 169
98 88 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 946 978 1162 1194 2137 2167 2169 2199 946
Size: 32 184 32 943 30 2 30 3042
Fragments arranged by size:
3042 943 184 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1334 1334
Size: 4295
KasI G'GCGC_C
Cuts at: 172 628 814 1203 1725 1973 172
Size: 456 186 389 522 248 2494
Fragments arranged by size:
2494 522 456 389 248 186
KpnI G_GTAC'C
Cuts at: 522 1315 522
Size: 793 3502
MluI A'CGCG_T
Cuts at: 3728 3728
Size: 4295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 179 1139 2966 3150 179
Size: 960 1827 184 1324
Fragments arranged by size:
1827 1324 960 184
MscI TGG'CCA
Cuts at: 577 2056 577
Size: 1479 2816
MslI CAynn'nnrTG
Cuts at: 511 850 1084 1123 2284 2411 511
Size: 339 234 39 1161 127 2395
Fragments arranged by size:
2395 1161 339 234 127 39
MspA1I CmG'CkG
Cuts at: 178 225 979 1098 1199 1262 1406 1720 2080
Size: 47 754 119 101 63 144 314 360
Cuts at: 2080 3093 3338 178
Size: 1013 245 1135
Fragments arranged by size:
1135 1013 754 360 314 245 144 119
101 63 47
NaeI GCC'GGC
Cuts at: 330 351 2476 330
Size: 21 2125 2149
Fragments arranged by size:
2149 2125 21
NarI GG'CG_CC
Cuts at: 173 629 815 1204 1726 1974 173
Size: 456 186 389 522 248 2494
Fragments arranged by size:
2494 522 456 389 248 186
NcoI C'CATG_G
Cuts at: 572 572
Size: 4295
NgoMIV G'CCGG_C
Cuts at: 328 349 2474 328
Size: 21 2125 2149
Fragments arranged by size:
2149 2125 21
NotI GC'GGCC_GC
Cuts at: 1158 1158
Size: 4295
NruI TCG'CGA
Cuts at: 1182 1182
Size: 4295
NspI r_CATG'y
Cuts at: 441 555 1348 1848 2755 441
Size: 114 793 500 907 1981
Fragments arranged by size:
1981 907 793 500 114
PciI A'CATG_T
Cuts at: 2751 2751
Size: 4295
PflMI CCAn_nnn'nTGG
Cuts at: 489 489
Size: 4295
PmeI GTTT'AAAC
Cuts at: 1294 1294
Size: 4295
PmlI CAC'GTG
Cuts at: 1121 1121
Size: 4295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3434 3466 3434
Size: 32 4263
PpuMI rG'GwC_Cy
Cuts at: 1101 1101
Size: 4295
PstI C_TGCA'G
Cuts at: 734 752 1073 1342 2027 734
Size: 18 321 269 685 3002
Fragments arranged by size:
3002 685 321 269 18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4295
PvuII CAG'CTG
Cuts at: 1720 2080 1720
Size: 360 3935
RsrII CG'GwC_CG
Cuts at: 2490 2490
Size: 4295
SacI G_AGCT'C
Cuts at: 1285 1309 1285
Size: 24 4271
SacII CC_GC'GG
Cuts at: 179 1099 1263 179
Size: 920 164 3211
Fragments arranged by size:
3211 920 164
SalI G'TCGA_C
Cuts at: 1332 1332
Size: 4295
SapI GCTCTTCn'nnn_
Cuts at: 2318 2528 2318
Size: 210 4085
SbfI CC_TGCA'GG
Cuts at: 1342 1342
Size: 4295
ScaI AGT'ACT
Cuts at: 1075 3812 1075
Size: 2737 1558
SfcI C'TryA_G
Cuts at: 33 730 748 1069 1164 1338 2023 3016 3207
Size: 697 18 321 95 174 685 993 191
Cuts at: 3207 33
Size: 1121
Fragments arranged by size:
1121 993 697 685 321 191 174 95
18
SfoI GGC'GCC
Cuts at: 174 630 816 1205 1727 1975 174
Size: 456 186 389 522 248 2494
Fragments arranged by size:
2494 522 456 389 248 186
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4295
SmaI CCC'GGG
Cuts at: 1317 1317
Size: 4295
SmlI C'TyrA_G
Cuts at: 313 1450 2365 2857 3119 313
Size: 1137 915 492 262 1489
Fragments arranged by size:
1489 1137 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4295
SphI G_CATG'C
Cuts at: 555 1348 1848 555
Size: 793 500 3002
Fragments arranged by size:
3002 793 500
StuI AGG'CCT
Cuts at: 673 673
Size: 4295
StyI C'CwwG_G
Cuts at: 509 572 1429 1783 509
Size: 63 857 354 3021
Fragments arranged by size:
3021 857 354 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 423 1878 2011 2470 2679 423
Size: 1455 133 459 209 2039
Fragments arranged by size:
2039 1455 459 209 133
TatI w'GTAC_w
Cuts at: 1073 3810 1073
Size: 2737 1558
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1012 1044 1234 1266 3812 3844 1012
Size: 32 190 32 2546 32 1463
Fragments arranged by size:
2546 1463 190 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2092 2092
Size: 4295
XbaI T'CTAG_A
Cuts at: 1326 1326
Size: 4295
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 543 579 1790 543
Size: 36 1211 3048
Fragments arranged by size:
3048 1211 36
XmaI C'CCGG_G
Cuts at: 1315 1315
Size: 4295
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NruI
NspI PciI PflMI PmeI PmlI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AvrII BaeI BcgI BfrBI BmtI BsaXI BsiWI BsmI
BsmBI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI CspCI
EcoRV FseI HindIII HpaI MfeI NdeI NheI NsiI
PacI PasI PfoI PshAI PsiI PspOMI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI