Restriction map for
pF1KE2646
(Circular) (Six-base) MAPSORT of: pF1KE2646.seq Check: 2841 from: 1 to: 9860
pF1KE2646 9860 bp
With 182 enzymes: SgfI *
November 11, 2016 16:52 ..
AatII G_ACGT'C
Cuts at: 5747 5747
Size: 9860
AccI GT'mk_AC
Cuts at: 3326 6683 6898 3326
Size: 3357 215 6288
Fragments arranged by size:
6288 3357 215
Acc65I G'GTAC_C
Cuts at: 974 2018 4608 6876 974
Size: 1044 2590 2268 3958
Fragments arranged by size:
3958 2590 2268 1044
AclI AA'CG_TT
Cuts at: 3497 9700 3497
Size: 6203 3657
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3591 3757 5723 5795 7685 8117 8864 9132 3591
Size: 166 1966 72 1890 432 747 268 4319
Fragments arranged by size:
4319 1966 1890 747 432 268 166 72
AfeI AGC'GCT
Cuts at: 816 1318 4789 816
Size: 502 3471 5887
Fragments arranged by size:
5887 3471 502
AflII C'TTAA_G
Cuts at: 5655 5655
Size: 9860
AflIII A'CryG_T
Cuts at: 2036 4031 4277 6176 7212 8316 9293 2036
Size: 1995 246 1899 1036 1104 977 2603
Fragments arranged by size:
2603 1995 1899 1104 1036 977 246
AgeI A'CCGG_T
Cuts at: 8248 8248
Size: 9860
AhdI GACnn_n'nnGTC
Cuts at: 2627 3418 5271 2627
Size: 791 1853 7216
Fragments arranged by size:
7216 1853 791
AleI CACnn'nnGTG
Cuts at: 3283 3283
Size: 9860
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2981 3919 4534 6259 2981
Size: 938 615 1725 6582
Fragments arranged by size:
6582 1725 938 615
AlwNI CAG_nnn'CTG
Cuts at: 1995 3136 3552 7165 8732 1995
Size: 1141 416 3613 1567 3123
Fragments arranged by size:
3613 3123 1567 1141 416
ApaI G_GGCC'C
Cuts at: 271 634 936 1252 2311 2723 4253 4348 4411
Size: 363 302 316 1059 412 1530 95 63
Cuts at: 4411 4549 5795 271
Size: 138 1246 4336
Fragments arranged by size:
4336 1530 1246 1059 412 363 316 302
138 95 63
ApaLI G'TGCA_C
Cuts at: 3814 8630 3814
Size: 4816 5044
ApoI r'AATT_y
Cuts at: 5195 5624 6436 6748 6864 9213 9469 5195
Size: 429 812 312 116 2349 256 5586
Fragments arranged by size:
5586 2349 812 429 312 256 116
AscI GG'CGCG_CC
Cuts at: 545 545
Size: 9860
AseI AT'TA_AT
Cuts at: 20 5081 20
Size: 5061 4799
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9860
AvaI C'yCGr_G
Cuts at: 270 627 714 760 889 1252 1420 1807 2301
Size: 357 87 46 129 363 168 387 494
Cuts at: 2301 2413 3226 3663 4164 4341 4410 6157 6880
Size: 112 813 437 501 177 69 1747 723
Cuts at: 6880 270
Size: 3250
Fragments arranged by size:
3250 1747 813 723 501 494 437 387
363 357 177 168 129 112 87 69
46
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1762 1795 1762
Size: 33 9827
BamHI G'GATC_C
Cuts at: 1662 3667 6885 1662
Size: 2005 3218 4637
Fragments arranged by size:
4637 3218 2005
BanI G'GyrC_C
Cuts at: 124 142 589 724 974 1546 1618 2018 2691
Size: 18 447 135 250 572 72 400 673
Cuts at: 2691 3087 3105 3502 4187 4566 4608 4764 5639
Size: 396 18 397 685 379 42 156 875
Cuts at: 5639 6876 7290 7538 7573 124
Size: 1237 414 248 35 2411
Fragments arranged by size:
2411 1237 875 685 673 572 447 414
400 397 396 379 250 248 156 135
72 42 35 18 18
BanII G_rGCy'C
Cuts at: 271 277 634 936 1189 1216 1252 1615 2200
Size: 6 357 302 253 27 36 363 585
Cuts at: 2200 2311 2723 3526 3593 3938 3989 4253 4348
Size: 111 412 803 67 345 51 264 95
Cuts at: 4348 4411 4549 5795 5876 6874 7904 271
Size: 63 138 1246 81 998 1030 2227
Fragments arranged by size:
2227 1246 1030 998 803 585 412 363
357 345 302 264 253 138 111 95
81 67 63 51 36 27 6
BbeI G_GCGC'C
Cuts at: 128 593 728 1550 3091 3109 4570 4768 5643
Size: 465 135 822 1541 18 1461 198 875
Cuts at: 5643 7294 7542 128
Size: 1651 248 2446
Fragments arranged by size:
2446 1651 1541 1461 875 822 465 248
198 135 18
BbsI GAAGACnn'nnnn_
Cuts at: 2108 3597 5342 5363 5374 5783 2108
Size: 1489 1745 21 11 409 6185
Fragments arranged by size:
6185 1745 1489 409 21 11
BbvCI CC'TCA_GC
Cuts at: 551 1276 1724 1835 4487 4683 551
Size: 725 448 111 2652 196 5728
Fragments arranged by size:
5728 2652 725 448 196 111
BciVI GTATCCnnnnn_n'
Cuts at: 1790 2392 4154 4391 5836 6388 7758 8519 9860
Size: 602 1762 237 1445 552 1370 761 1341
Cuts at: 9860 1790
Size: 1790
Fragments arranged by size:
1790 1762 1445 1370 1341 761 602 552
237
BclI T'GATC_A
Cuts at: 864 5241 7380 864
Size: 4377 2139 3344
Fragments arranged by size:
4377 3344 2139
BfrBI ATG'CAT
Cuts at: 2278 2580 2754 5942 2278
Size: 302 174 3188 6196
Fragments arranged by size:
6196 3188 302 174
BglI GCCn_nnn'nGGC
Cuts at: 842 931 1019 1141 1256 1297 1570 1579 1766
Size: 89 88 122 115 41 273 9 187
Cuts at: 1766 1931 4271 4553 842
Size: 165 2340 282 6149
Fragments arranged by size:
6149 2340 282 273 187 165 122 115
89 88 41 9
BglII A'GATC_T
Cuts at: 2062 2145 7375 9412 2062
Size: 83 5230 2037 2510
Fragments arranged by size:
5230 2510 2037 83
BlpI GC'TnA_GC
Cuts at: 643 1210 2199 5933 6972 643
Size: 567 989 3734 1039 3531
Fragments arranged by size:
3734 3531 1039 989 567
Bme1580I G_kGCm'C
Cuts at: 271 634 736 761 936 1252 2311 2696 2723
Size: 363 102 25 175 316 1059 385 27
Cuts at: 2723 2792 3818 4190 4253 4348 4411 4549 5795
Size: 69 1026 372 63 95 63 138 1246
Cuts at: 5795 6283 7485 7578 8634 271
Size: 488 1202 93 1056 1497
Fragments arranged by size:
1497 1246 1202 1059 1056 1026 488 385
372 363 316 175 138 102 95 93
69 63 63 27 25
BmgBI CAC'GTC
Cuts at: 4653 4653
Size: 9860
BmrI ACTGGGnnnn_n'
Cuts at: 1461 1545 1998 2690 2855 3275 3687 5124 6475
Size: 84 453 692 165 420 412 1437 1351
Cuts at: 6475 6612 7072 7153 7488 9632 1461
Size: 137 460 81 335 2144 1689
Fragments arranged by size:
2144 1689 1437 1351 692 460 453 420
412 335 165 137 84 81
BmtI G_CTAG'C
Cuts at: 6718 6718
Size: 9860
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3375 3377 8227 3375
Size: 2 4850 5008
Fragments arranged by size:
5008 4850 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1748 1871 2277 2652 3625 4458 4743 4816 5884
Size: 123 406 375 973 833 285 73 1068
Cuts at: 5884 6376 1748
Size: 492 5232
Fragments arranged by size:
5232 1068 973 833 492 406 375 285
123 73
Bpu10I CC'TnA_GC
Cuts at: 551 1276 1724 1835 4487 4683 551
Size: 725 448 111 2652 196 5728
Fragments arranged by size:
5728 2652 725 448 196 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 2469 2598 3354 3492 6186 7036 7915 8407
Size: 2303 129 756 138 2694 850 879 492
Cuts at: 8407 8705 166
Size: 298 1321
Fragments arranged by size:
2694 2303 1321 879 850 756 492 298
138 129
BsaI GGTCTCn'nnnn_
Cuts at: 1674 5213 6352 9564 1674
Size: 3539 1139 3212 1970
Fragments arranged by size:
3539 3212 1970 1139
BsaAI yAC'GTr
Cuts at: 5063 7213 5063
Size: 2150 7710
BsaBI GATnn'nnATC
Cuts at: 6 7374 9411 6
Size: 7368 2037 455
Fragments arranged by size:
7368 2037 455
BsaHI Gr'CG_yC
Cuts at: 125 590 725 1547 3088 3106 4567 4732 4765
Size: 465 135 822 1541 18 1461 165 33
Cuts at: 4765 5414 5640 5744 7291 7539 8241 9737 125
Size: 649 226 104 1547 248 702 1496 248
Fragments arranged by size:
1547 1541 1496 1461 822 702 649 465
248 248 226 165 135 104 33 18
BsaWI w'CCGG_w
Cuts at: 4051 5846 7049 7570 8248 8522 8669 9080 4051
Size: 1795 1203 521 678 274 147 411 4831
Fragments arranged by size:
4831 1795 1203 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 898 928 4917 4947 898
Size: 30 3989 30 5811
Fragments arranged by size:
5811 3989 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 204 207 210 213 216 249 1067 1070 1073
Size: 3 3 3 3 33 818 3 3
Cuts at: 1073 1085 1088 1091 1094 1097 1100 1103 1118
Size: 12 3 3 3 3 3 3 15
Cuts at: 1118 2063 3243 3601 5314 5356 5365 5621 5885
Size: 945 1180 358 1713 42 9 256 264
Cuts at: 5885 6115 6404 204
Size: 230 289 3660
Fragments arranged by size:
3660 1713 1180 945 818 358 289 264
256 230 42 33 15 12 9 3
3 3 3 3 3 3 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 850 1384 1435 2009 2676 2812 3294 3750 4254
Size: 534 51 574 667 136 482 456 504
Cuts at: 4254 4768 4773 5224 7285 8043 8620 9116 850
Size: 514 5 451 2061 758 577 496 1594
Fragments arranged by size:
2061 1594 758 667 577 574 534 514
504 496 482 456 451 136 51 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3536 5542 5554 6250 6580 6714 3536
Size: 2006 12 696 330 134 6682
Fragments arranged by size:
6682 2006 696 330 134 12
BsiEI CG_ry'CG
Cuts at: 104 134 702 752 785 846 1136 1289 1517
Size: 30 568 50 33 61 290 153 228
Cuts at: 1517 1583 3330 4116 7402 7448 8656 104
Size: 66 1747 786 3286 46 1208 1308
Fragments arranged by size:
3286 1747 1308 1208 786 568 290 228
153 66 61 50 46 33 30
BsiHKAI G_wGCw'C
Cuts at: 713 1216 2200 3526 3818 5594 6081 6874 7652
Size: 503 984 1326 292 1776 487 793 778
Cuts at: 7652 7842 8634 713
Size: 190 792 1939
Fragments arranged by size:
1939 1776 1326 984 793 792 778 503
487 292 190
BsmI GAATG_Cn'
Cuts at: 1829 2807 1829
Size: 978 8882
BsmBI CGTCTCn'nnnn_
Cuts at: 280 5879 280
Size: 5599 4261
Bsp1286I G_dGCh'C
Cuts at: 271 277 634 713 736 761 936 1189 1216
Size: 6 357 79 23 25 175 253 27
Cuts at: 1216 1252 1615 2200 2311 2696 2723 2792 3526
Size: 36 363 585 111 385 27 69 734
Cuts at: 3526 3593 3818 3938 3989 4190 4253 4348 4411
Size: 67 225 120 51 201 63 95 63
Cuts at: 4411 4549 5594 5795 5876 6081 6283 6874 7485
Size: 138 1045 201 81 205 202 591 611
Cuts at: 7485 7578 7652 7842 7904 8634 271
Size: 93 74 190 62 730 1497
Fragments arranged by size:
1497 1045 734 730 611 591 585 385
363 357 253 225 205 202 201 201
190 175 138 120 111 95 93 81
79 74 69 67 63 63 62 51
36 27 27 25 23 6
BspEI T'CCGG_A
Cuts at: 4051 9080 4051
Size: 5029 4831
BspHI T'CATG_A
Cuts at: 4629 9036 4629
Size: 4407 5453
BspMI ACCTGCnnnn'nnnn_
Cuts at: 5656 6910 7124 7426 7807 5656
Size: 1254 214 302 381 7709
Fragments arranged by size:
7709 1254 381 302 214
BsrBI CCG'CTC
Cuts at: 50 161 830 1114 6743 8152 8206 9690 9855
Size: 111 669 284 5629 1409 54 1484 165
Cuts at: 9855 50
Size: 55
Fragments arranged by size:
5629 1484 1409 669 284 165 111 55
54
BsrDI GCAATG_nn'
Cuts at: 4064 4518 5767 5994 7772 4064
Size: 454 1249 227 1778 6152
Fragments arranged by size:
6152 1778 1249 454 227
BsrFI r'CCGG_y
Cuts at: 139 690 747 1013 1291 1324 6767 7858 8039
Size: 551 57 266 278 33 5443 1091 181
Cuts at: 8039 8248 139
Size: 209 1751
Fragments arranged by size:
5443 1751 1091 551 278 266 209 181
57 33
BsrGI T'GTAC_A
Cuts at: 4034 4280 4316 4034
Size: 246 36 9578
Fragments arranged by size:
9578 246 36
BssHII G'CGCG_C
Cuts at: 545 1439 545
Size: 894 8966
BssSI C'ACGA_G
Cuts at: 2223 4267 6055 6760 8131 8489 2223
Size: 2044 1788 705 1371 358 3594
Fragments arranged by size:
3594 2044 1788 1371 705 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1995 2209 7237 1995
Size: 214 5028 4618
Fragments arranged by size:
5028 4618 214
BstBI TT'CG_AA
Cuts at: 8221 8221
Size: 9860
BstEII G'GTnAC_C
Cuts at: 3730 4881 3730
Size: 1151 8709
BstXI CCAn_nnnn'nTGG
Cuts at: 2755 2779 3700 5137 2755
Size: 24 921 1437 7478
Fragments arranged by size:
7478 1437 921 24
BstYI r'GATC_y
Cuts at: 1 1642 1662 2062 2145 3667 4418 6300 6885
Size: 1641 20 400 83 1522 751 1882 585
Cuts at: 6885 7352 7375 7710 7956 8968 9054 9066 9412
Size: 467 23 335 246 1012 86 12 346
Cuts at: 9412 1
Size: 449
Fragments arranged by size:
1882 1641 1522 1012 751 585 467 449
400 346 335 246 86 83 23 20
12
Bsu36I CC'TnA_GG
Cuts at: 5009 5009
Size: 9860
BtgI C'CryG_G
Cuts at: 107 128 230 281 1136 1193 1517 1634 1814
Size: 21 102 51 855 57 324 117 180
Cuts at: 1814 2174 3113 3383 3713 4862 6500 6719 107
Size: 360 939 270 330 1149 1638 219 3248
Fragments arranged by size:
3248 1638 1149 939 855 360 330 324
270 219 180 117 102 57 51 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1621 5379 5393 5892 5952 7810 7991 8154 1621
Size: 3758 14 499 60 1858 181 163 3327
Fragments arranged by size:
3758 3327 1858 499 181 163 60 14
BtsI GCAGTG_nn'
Cuts at: 2957 3519 4741 7103 2957
Size: 562 1222 2362 5714
Fragments arranged by size:
5714 2362 1222 562
ClaI AT'CG_AT
Cuts at: 5738 5738
Size: 9860
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 171 206 171
Size: 35 9825
DraI TTT'AAA
Cuts at: 6859 6859
Size: 9860
DrdI GACnn_nn'nnGTC
Cuts at: 3693 4737 7566 8424 3693
Size: 1044 2829 858 5129
Fragments arranged by size:
5129 2829 1044 858
EaeI y'GGCC_r
Cuts at: 131 278 612 699 782 843 1133 1286 1326
Size: 147 334 87 83 61 290 153 40
Cuts at: 1326 1503 1514 1580 1817 3768 5758 6191 6407
Size: 177 11 66 237 1951 1990 433 216
Cuts at: 6407 6701 6722 7445 7619 8010 8037 9382 131
Size: 294 21 723 174 391 27 1345 609
Fragments arranged by size:
1990 1951 1345 723 609 433 391 334
294 290 237 216 177 174 153 147
87 83 66 61 40 27 21 11
EagI C'GGCC_G
Cuts at: 131 699 782 843 1133 1286 1514 1580 7445
Size: 568 83 61 290 153 228 66 5865
Cuts at: 7445 131
Size: 2546
Fragments arranged by size:
5865 2546 568 290 228 153 83 66
61
EarI CTCTTCn'nnn_
Cuts at: 258 1672 3344 5699 7883 8093 258
Size: 1414 1672 2355 2184 210 2025
Fragments arranged by size:
2355 2184 2025 1672 1414 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 890 900 906 1064 8068 8378 8524 9670 890
Size: 10 6 158 7004 310 146 1146 1080
Fragments arranged by size:
7004 1146 1080 310 158 146 10 6
EcoICRI GAG'CTC
Cuts at: 1214 2198 3524 6872 1214
Size: 984 1326 3348 4202
Fragments arranged by size:
4202 3348 1326 984
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1748 1871 2277 2652 3591 3625 3757 4458 4743
Size: 123 406 375 939 34 132 701 285
Cuts at: 4743 4816 5723 5795 5884 6376 7685 8117 8864
Size: 73 907 72 89 492 1309 432 747
Cuts at: 8864 9132 1748
Size: 268 2476
Fragments arranged by size:
2476 1309 939 907 747 701 492 432
406 375 285 268 132 123 89 73
72 34
EcoNI CCTnn'n_nnAGG
Cuts at: 1279 4389 4577 1279
Size: 3110 188 6562
Fragments arranged by size:
6562 3110 188
EcoO109I rG'GnC_Cy
Cuts at: 932 933 1249 2307 2719 3945 4156 4250 4407
Size: 1 316 1058 412 1226 211 94 157
Cuts at: 4407 4483 4545 4546 5792 6319 6999 932
Size: 76 62 1 1246 527 680 3793
Fragments arranged by size:
3793 1246 1226 1058 680 527 412 316
211 157 94 76 62 1 1
EcoRI G'AATT_C
Cuts at: 5195 6748 6864 5195
Size: 1553 116 8191
Fragments arranged by size:
8191 1553 116
EcoRV GAT'ATC
Cuts at: 4998 6780 6788 4998
Size: 1782 8 8070
Fragments arranged by size:
8070 1782 8
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2639 3966 5835 7879 2639
Size: 1327 1869 2044 4620
Fragments arranged by size:
4620 2044 1869 1327
FspI TGC'GCA
Cuts at: 7641 7641
Size: 9860
HaeII r_GCGC'y
Cuts at: 128 155 200 593 728 818 1320 1550 3091
Size: 27 45 393 135 90 502 230 1541
Cuts at: 3091 3109 4086 4266 4570 4768 4791 5643 6508
Size: 18 977 180 304 198 23 852 865
Cuts at: 6508 7294 7542 8564 9543 128
Size: 786 248 1022 979 445
Fragments arranged by size:
1541 1022 979 977 865 852 786 502
445 393 304 248 230 198 180 135
90 45 27 23 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1350 1382 1875 1907 2613 2645 3142 3174 3220
Size: 32 493 32 706 32 497 32 46
Cuts at: 3220 3252 3404 3436 3811 3843 4611 4643 5257
Size: 32 152 32 375 32 768 32 614
Cuts at: 5257 5289 5734 5766 7702 7732 7734 7764 1350
Size: 32 445 32 1936 30 2 30 3446
Fragments arranged by size:
3446 1936 768 706 614 497 493 445
375 152 46 32 32 32 32 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3327 3698 5508 6899 3327
Size: 371 1810 1391 6288
Fragments arranged by size:
6288 1810 1391 371
HindIII A'AGCT_T
Cuts at: 6588 6588
Size: 9860
KasI G'GCGC_C
Cuts at: 124 589 724 1546 3087 3105 4566 4764 5639
Size: 465 135 822 1541 18 1461 198 875
Cuts at: 5639 7290 7538 124
Size: 1651 248 2446
Fragments arranged by size:
2446 1651 1541 1461 875 822 465 248
198 135 18
KpnI G_GTAC'C
Cuts at: 978 2022 4612 6880 978
Size: 1044 2590 2268 3958
Fragments arranged by size:
3958 2590 2268 1044
MfeI C'AATT_G
Cuts at: 1784 1784
Size: 9860
MluI A'CGCG_T
Cuts at: 9293 9293
Size: 9860
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 536 1780 3435 6334 8531 8715 536
Size: 1244 1655 2899 2197 184 1681
Fragments arranged by size:
2899 2197 1681 1655 1244 184
MscI TGG'CCA
Cuts at: 6193 6409 6724 7621 6193
Size: 216 315 897 8432
Fragments arranged by size:
8432 897 315 216
MslI CAynn'nnrTG
Cuts at: 371 392 1243 3283 3343 3901 5947 6556 6649
Size: 21 851 2040 60 558 2046 609 93
Cuts at: 6649 7849 7976 371
Size: 1200 127 2255
Fragments arranged by size:
2255 2046 2040 1200 851 609 558 127
93 60 21
MspA1I CmG'CkG
Cuts at: 130 232 241 639 1067 1138 1195 1371 1519
Size: 102 9 398 428 71 57 176 148
Cuts at: 1519 1561 1776 2711 2812 4461 5419 6793 6971
Size: 42 215 935 101 1649 958 1374 178
Cuts at: 6971 7285 7645 8658 8903 130
Size: 314 360 1013 245 1087
Fragments arranged by size:
1649 1374 1087 1013 958 935 428 398
360 314 245 215 178 176 148 102
101 71 57 42 9
NaeI GCC'GGC
Cuts at: 141 692 1015 1293 1326 8041 141
Size: 551 323 278 33 6715 1960
Fragments arranged by size:
6715 1960 551 323 278 33
NarI GG'CG_CC
Cuts at: 125 590 725 1547 3088 3106 4567 4765 5640
Size: 465 135 822 1541 18 1461 198 875
Cuts at: 5640 7291 7539 125
Size: 1651 248 2446
Fragments arranged by size:
2446 1651 1541 1461 875 822 465 248
198 135 18
NcoI C'CATG_G
Cuts at: 107 281 1634 1814 3113 3713 6500 6719 107
Size: 174 1353 180 1299 600 2787 219 3248
Fragments arranged by size:
3248 2787 1353 1299 600 219 180 174
NdeI CA'TA_TG
Cuts at: 4232 4232
Size: 9860
NgoMIV G'CCGG_C
Cuts at: 139 690 1013 1291 1324 8039 139
Size: 551 323 278 33 6715 1960
Fragments arranged by size:
6715 1960 551 323 278 33
NheI G'CTAG_C
Cuts at: 6714 6714
Size: 9860
NotI GC'GGCC_GC
Cuts at: 131 699 843 1133 1286 1514 1580 131
Size: 568 144 290 153 228 66 8411
Fragments arranged by size:
8411 568 290 228 153 144 66
NsiI A_TGCA'T
Cuts at: 2280 2582 2756 5944 2280
Size: 302 174 3188 6196
Fragments arranged by size:
6196 3188 302 174
NspI r_CATG'y
Cuts at: 2040 2278 2584 2877 3906 4026 4035 4245 4281
Size: 238 306 293 1029 120 9 210 36
Cuts at: 4281 4290 4809 6654 6913 7413 8320 2040
Size: 9 519 1845 259 500 907 3580
Fragments arranged by size:
3580 1845 1029 907 519 500 306 293
259 238 210 120 36 9 9
PasI CC'CwG_GG
Cuts at: 729 1647 2716 2793 5788 729
Size: 918 1069 77 2995 4801
Fragments arranged by size:
4801 2995 1069 918 77
PciI A'CATG_T
Cuts at: 2036 4031 4277 8316 2036
Size: 1995 246 4039 3580
Fragments arranged by size:
4039 3580 1995 246
PflMI CCAn_nnn'nTGG
Cuts at: 2812 2836 5277 2812
Size: 24 2441 7395
Fragments arranged by size:
7395 2441 24
PfoI T'CCnGG_A
Cuts at: 788 2835 6380 788
Size: 2047 3545 4268
Fragments arranged by size:
4268 3545 2047
PmeI GTTT'AAAC
Cuts at: 6859 6859
Size: 9860
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2049 2081 3049 3081 8999 9031 2049
Size: 32 968 32 5918 32 2878
Fragments arranged by size:
5918 2878 968 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3945 6319 3945
Size: 2374 7486
PspOMI G'GGCC_C
Cuts at: 267 630 932 1248 2307 2719 4249 4344 4407
Size: 363 302 316 1059 412 1530 95 63
Cuts at: 4407 4545 5791 267
Size: 138 1246 4336
Fragments arranged by size:
4336 1530 1246 1059 412 363 316 302
138 95 63
PspXI vC'TCGA_Gb
Cuts at: 6157 6157
Size: 9860
PstI C_TGCA'G
Cuts at: 959 4466 5424 6130 6291 6907 7592 959
Size: 3507 958 706 161 616 685 3227
Fragments arranged by size:
3507 3227 958 706 685 616 161
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9860
PvuII CAG'CTG
Cuts at: 1371 2812 6793 7285 7645 1371
Size: 1441 3981 492 360 3586
Fragments arranged by size:
3981 3586 1441 492 360
RsrII CG'GwC_CG
Cuts at: 1627 4350 8055 1627
Size: 2723 3705 3432
Fragments arranged by size:
3705 3432 2723
SacI G_AGCT'C
Cuts at: 1216 2200 3526 6874 1216
Size: 984 1326 3348 4202
Fragments arranged by size:
4202 3348 1326 984
SacII CC_GC'GG
Cuts at: 131 233 1139 1196 1520 131
Size: 102 906 57 324 8471
Fragments arranged by size:
8471 906 324 102 57
SalI G'TCGA_C
Cuts at: 3325 6897 3325
Size: 3572 6288
SanDI GG'GwC_CC
Cuts at: 6319 6319
Size: 9860
SapI GCTCTTCn'nnn_
Cuts at: 5699 7883 8093 5699
Size: 2184 210 7466
Fragments arranged by size:
7466 2184 210
SbfI CC_TGCA'GG
Cuts at: 6907 6907
Size: 9860
ScaI AGT'ACT
Cuts at: 5164 9377 5164
Size: 4213 5647
SfcI C'TryA_G
Cuts at: 33 424 955 1008 2336 2706 3042 3096 4386
Size: 391 531 53 1328 370 336 54 1290
Cuts at: 4386 4462 5420 6126 6287 6903 7588 8581 8772
Size: 76 958 706 161 616 685 993 191
Cuts at: 8772 33
Size: 1121
Fragments arranged by size:
1328 1290 1121 993 958 706 685 616
531 391 370 336 191 161 76 54
53
SfiI GGCCn_nnn'nGGCC
Cuts at: 842 1579 1766 4553 842
Size: 737 187 2787 6149
Fragments arranged by size:
6149 2787 737 187
SfoI GGC'GCC
Cuts at: 126 591 726 1548 3089 3107 4568 4766 5641
Size: 465 135 822 1541 18 1461 198 875
Cuts at: 5641 7292 7540 126
Size: 1651 248 2446
Fragments arranged by size:
2446 1651 1541 1461 875 822 465 248
198 135 18
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9860
SmaI CCC'GGG
Cuts at: 272 629 716 762 891 1254 1809 2303 2415
Size: 357 87 46 129 363 555 494 112
Cuts at: 2415 4412 6882 272
Size: 1997 2470 3250
Fragments arranged by size:
3250 2470 1997 555 494 363 357 129
112 87 46
SmlI C'TyrA_G
Cuts at: 181 2448 2613 3369 3507 5655 6157 6165 7015
Size: 2267 165 756 138 2148 502 8 850
Cuts at: 7015 7930 8422 8684 181
Size: 915 492 262 1357
Fragments arranged by size:
2267 2148 1357 915 850 756 502 492
262 165 138 8
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9860
SphI G_CATG'C
Cuts at: 2584 3906 4026 6913 7413 2584
Size: 1322 120 2887 500 5031
Fragments arranged by size:
5031 2887 1322 500 120
SrfI GCCC'GGGC
Cuts at: 272 272
Size: 9860
SspI AAT'ATT
Cuts at: 5085 5958 5085
Size: 873 8987
StuI AGG'CCT
Cuts at: 6017 6017
Size: 9860
StyI C'CwwG_G
Cuts at: 107 281 593 1634 1814 2232 3113 3713 4152
Size: 174 312 1041 180 418 881 600 439
Cuts at: 4152 5276 6500 6525 6704 6719 6994 7348 107
Size: 1124 1224 25 179 15 275 354 2619
Fragments arranged by size:
2619 1224 1124 1041 881 600 439 418
354 312 275 180 179 174 25 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 160 1644 3344 4130 5525 5815 6318 7443 7576
Size: 1484 1700 786 1395 290 503 1125 133
Cuts at: 7576 8035 8244 160
Size: 459 209 1776
Fragments arranged by size:
1776 1700 1484 1395 1125 786 503 459
290 209 133
TatI w'GTAC_w
Cuts at: 1424 3802 4034 4280 4292 4316 5162 6831 9375
Size: 2378 232 246 12 24 846 1669 2544
Cuts at: 9375 1424
Size: 1909
Fragments arranged by size:
2544 2378 1909 1669 846 246 232 24
12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 898 930 4418 4450 4917 4949 9377 9409 898
Size: 32 3488 32 467 32 4428 32 1349
Fragments arranged by size:
4428 3488 1349 467 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 7657 7657
Size: 9860
XbaI T'CTAG_A
Cuts at: 6891 6891
Size: 9860
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2356 2581 6726 7355 2356
Size: 225 4145 629 4861
Fragments arranged by size:
4861 4145 629 225
XhoI C'TCGA_G
Cuts at: 6157 6157
Size: 9860
XmaI C'CCGG_G
Cuts at: 270 627 714 760 889 1252 1807 2301 2413
Size: 357 87 46 129 363 555 494 112
Cuts at: 2413 4410 6880 270
Size: 1997 2470 3250
Fragments arranged by size:
3250 2470 1997 555 494 363 357 129
112 87 46
ZraI GAC'GTC
Cuts at: 5745 5745
Size: 9860
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AloI AvrII BcgI BsiWI BstZ17I DraIII FseI
FspAI HpaI NruI PacI PmlI PshAI PsiI PsrI
SexAI SgrAI SnaBI SwaI XmnI