Restriction map for
pF1KE2649
(Circular) (Six-base) MAPSORT of: pF1KE2649.seq Check: 2105 from: 1 to: 3686
pF1KE2649 3686 bp
With 182 enzymes: SgfI *
November 11, 2016 16:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 656 656
Size: 3686
AccI GT'mk_AC
Cuts at: 724 724
Size: 3686
Acc65I G'GTAC_C
Cuts at: 702 702
Size: 3686
AclI AA'CG_TT
Cuts at: 3526 3526
Size: 3686
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 1511 1943 2690 2958 574
Size: 937 432 747 268 1302
Fragments arranged by size:
1302 937 747 432 268
AflIII A'CryG_T
Cuts at: 157 1038 2142 3119 157
Size: 881 1104 977 724
Fragments arranged by size:
1104 977 881 724
AgeI A'CCGG_T
Cuts at: 2074 2074
Size: 3686
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 688 688
Size: 3686
AlwNI CAG_nnn'CTG
Cuts at: 529 991 2558 529
Size: 462 1567 1657
Fragments arranged by size:
1657 1567 462
ApaLI G'TGCA_C
Cuts at: 2456 2456
Size: 3686
ApoI r'AATT_y
Cuts at: 187 690 3039 3295 187
Size: 503 2349 256 578
Fragments arranged by size:
2349 578 503 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3686
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3686
AvaI C'yCGr_G
Cuts at: 706 706
Size: 3686
BamHI G'GATC_C
Cuts at: 711 711
Size: 3686
BanI G'GyrC_C
Cuts at: 399 702 1116 1364 1399 399
Size: 303 414 248 35 2686
Fragments arranged by size:
2686 414 303 248 35
BanII G_rGCy'C
Cuts at: 700 1730 700
Size: 1030 2656
BbeI G_GCGC'C
Cuts at: 1120 1368 1120
Size: 248 3438
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3652
BciVI GTATCCnnnnn_n'
Cuts at: 1584 2345 3686 1584
Size: 761 1341 1584
Fragments arranged by size:
1584 1341 761
BclI T'GATC_A
Cuts at: 1206 1206
Size: 3686
BglII A'GATC_T
Cuts at: 1201 3238 1201
Size: 2037 1649
BlpI GC'TnA_GC
Cuts at: 798 798
Size: 3686
Bme1580I G_kGCm'C
Cuts at: 1311 1404 2460 1311
Size: 93 1056 2537
Fragments arranged by size:
2537 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 898 979 1314 3458 898
Size: 81 335 2144 1126
Fragments arranged by size:
2144 1126 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2053 2053
Size: 3686
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 506 629 658 506
Size: 123 29 3534
Fragments arranged by size:
3534 123 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 862 1741 2233 2531 862
Size: 879 492 298 2017
Fragments arranged by size:
2017 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 366 3390 366
Size: 3024 662
BsaAI yAC'GTr
Cuts at: 1039 1039
Size: 3686
BsaBI GATnn'nnATC
Cuts at: 6 1200 3237 6
Size: 1194 2037 455
Fragments arranged by size:
2037 1194 455
BsaHI Gr'CG_yC
Cuts at: 1117 1365 2067 3563 1117
Size: 248 702 1496 1240
Fragments arranged by size:
1496 1240 702 248
BsaWI w'CCGG_w
Cuts at: 875 1396 2074 2348 2495 2906 875
Size: 521 678 274 147 411 1655
Fragments arranged by size:
1655 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1111 1869 2446 2942 1111
Size: 758 577 496 1855
Fragments arranged by size:
1855 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 566
Size: 3686
BsiEI CG_ry'CG
Cuts at: 104 313 1228 1274 2482 104
Size: 209 915 46 1208 1308
Fragments arranged by size:
1308 1208 915 209 46
BsiHKAI G_wGCw'C
Cuts at: 700 1478 1668 2460 700
Size: 778 190 792 1926
Fragments arranged by size:
1926 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 700 1311 1404 1478 1668 1730 2460 700
Size: 611 93 74 190 62 730 1926
Fragments arranged by size:
1926 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2906 2906
Size: 3686
BspHI T'CATG_A
Cuts at: 2862 2862
Size: 3686
BspMI ACCTGCnnnn'nnnn_
Cuts at: 656 736 950 1252 1633 656
Size: 80 214 302 381 2709
Fragments arranged by size:
2709 381 302 214 80
BsrBI CCG'CTC
Cuts at: 50 1978 2032 3516 3681 50
Size: 1928 54 1484 165 55
Fragments arranged by size:
1928 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1598 1598
Size: 3686
BsrFI r'CCGG_y
Cuts at: 1684 1865 2074 1684
Size: 181 209 3296
Fragments arranged by size:
3296 209 181
BssSI C'ACGA_G
Cuts at: 1957 2315 1957
Size: 358 3328
BstAPI GCAn_nnn'nTGC
Cuts at: 1063 1063
Size: 3686
BstBI TT'CG_AA
Cuts at: 2047 2047
Size: 3686
BstYI r'GATC_y
Cuts at: 1 711 1178 1201 1536 1782 2794 2880 2892
Size: 710 467 23 335 246 1012 86 12
Cuts at: 2892 3238 1
Size: 346 449
Fragments arranged by size:
1012 710 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 152 1636 1817 1980 152
Size: 1484 181 163 1858
Fragments arranged by size:
1858 1484 181 163
BtsI GCAGTG_nn'
Cuts at: 929 929
Size: 3686
DraI TTT'AAA
Cuts at: 685 685
Size: 3686
DrdI GACnn_nn'nnGTC
Cuts at: 1392 2250 1392
Size: 858 2828
EaeI y'GGCC_r
Cuts at: 1271 1445 1836 1863 3208 1271
Size: 174 391 27 1345 1749
Fragments arranged by size:
1749 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1271 1271
Size: 3686
EarI CTCTTCn'nnn_
Cuts at: 652 1709 1919 652
Size: 1057 210 2419
Fragments arranged by size:
2419 1057 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1894 2204 2350 3496 1894
Size: 310 146 1146 2084
Fragments arranged by size:
2084 1146 310 146
EcoICRI GAG'CTC
Cuts at: 698 698
Size: 3686
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 506 574 629 658 1511 1943 2690 2958 506
Size: 68 55 29 853 432 747 268 1234
Fragments arranged by size:
1234 853 747 432 268 68 55 29
EcoO109I rG'GnC_Cy
Cuts at: 825 825
Size: 3686
EcoRI G'AATT_C
Cuts at: 690 690
Size: 3686
EcoRV GAT'ATC
Cuts at: 170 170
Size: 3686
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1705 1705
Size: 3686
FspI TGC'GCA
Cuts at: 1467 1467
Size: 3686
HaeII r_GCGC'y
Cuts at: 1120 1368 2390 3369 1120
Size: 248 1022 979 1437
Fragments arranged by size:
1437 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 460 492 1528 1558 1560 1590 460
Size: 32 1036 30 2 30 2556
Fragments arranged by size:
2556 1036 32 30 30 2
HincII GTy'rAC
Cuts at: 725 725
Size: 3686
KasI G'GCGC_C
Cuts at: 1116 1364 1116
Size: 248 3438
KpnI G_GTAC'C
Cuts at: 706 706
Size: 3686
MluI A'CGCG_T
Cuts at: 3119 3119
Size: 3686
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2357 2541 2357
Size: 184 3502
MscI TGG'CCA
Cuts at: 1447 1447
Size: 3686
MslI CAynn'nnrTG
Cuts at: 238 1675 1802 238
Size: 1437 127 2122
Fragments arranged by size:
2122 1437 127
MspA1I CmG'CkG
Cuts at: 315 797 1111 1471 2484 2729 315
Size: 482 314 360 1013 245 1272
Fragments arranged by size:
1272 1013 482 360 314 245
NaeI GCC'GGC
Cuts at: 1867 1867
Size: 3686
NarI GG'CG_CC
Cuts at: 1117 1365 1117
Size: 248 3438
NgoMIV G'CCGG_C
Cuts at: 1865 1865
Size: 3686
NspI r_CATG'y
Cuts at: 161 739 1239 2146 161
Size: 578 500 907 1701
Fragments arranged by size:
1701 907 578 500
PciI A'CATG_T
Cuts at: 157 2142 157
Size: 1985 1701
PmeI GTTT'AAAC
Cuts at: 685 685
Size: 3686
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2825 2857 2825
Size: 32 3654
PstI C_TGCA'G
Cuts at: 285 733 1418 285
Size: 448 685 2553
Fragments arranged by size:
2553 685 448
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3686
PvuII CAG'CTG
Cuts at: 1111 1471 1111
Size: 360 3326
RsrII CG'GwC_CG
Cuts at: 1881 1881
Size: 3686
SacI G_AGCT'C
Cuts at: 700 700
Size: 3686
SalI G'TCGA_C
Cuts at: 723 723
Size: 3686
SapI GCTCTTCn'nnn_
Cuts at: 652 1709 1919 652
Size: 1057 210 2419
Fragments arranged by size:
2419 1057 210
SbfI CC_TGCA'GG
Cuts at: 733 733
Size: 3686
ScaI AGT'ACT
Cuts at: 3203 3203
Size: 3686
SfcI C'TryA_G
Cuts at: 33 281 729 1414 2407 2598 33
Size: 248 448 685 993 191 1121
Fragments arranged by size:
1121 993 685 448 248 191
SfoI GGC'GCC
Cuts at: 1118 1366 1118
Size: 248 3438
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3686
SmaI CCC'GGG
Cuts at: 708 708
Size: 3686
SmlI C'TyrA_G
Cuts at: 841 1756 2248 2510 841
Size: 915 492 262 2017
Fragments arranged by size:
2017 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3686
SphI G_CATG'C
Cuts at: 739 1239 739
Size: 500 3186
SspI AAT'ATT
Cuts at: 626 626
Size: 3686
StuI AGG'CCT
Cuts at: 599 599
Size: 3686
StyI C'CwwG_G
Cuts at: 820 1174 820
Size: 354 3332
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 387 1269 1402 1861 2070 387
Size: 882 133 459 209 2003
Fragments arranged by size:
2003 882 459 209 133
TatI w'GTAC_w
Cuts at: 3201 3201
Size: 3686
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3203 3235 3203
Size: 32 3654
Tth111I GACn'n_nGTC
Cuts at: 1483 1483
Size: 3686
XbaI T'CTAG_A
Cuts at: 717 717
Size: 3686
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1181 1181
Size: 3686
XmaI C'CCGG_G
Cuts at: 706 706
Size: 3686
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
XmnI ZraI