(Circular) (Six-base) MAPSORT of: pF1KE2654.seq Check: 4571 from: 1 to: 3950 pF1KE2654 3950 bp With 182 enzymes: SgfI * December 1, 2016 15:05 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 504 504 Size: 3950 AccI GT'mk_AC Cuts at: 299 988 299 Size: 689 3261 Acc65I G'GTAC_C Cuts at: 966 966 Size: 3950 AclI AA'CG_TT Cuts at: 944 3790 944 Size: 2846 1104 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 886 1775 2207 2954 3222 886 Size: 889 432 747 268 1614 Fragments arranged by size: 1614 889 747 432 268 AflIII A'CryG_T Cuts at: 221 528 1302 2406 3383 221 Size: 307 774 1104 977 788 Fragments arranged by size: 1104 977 788 774 307 AgeI A'CCGG_T Cuts at: 2338 2338 Size: 3950 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 904 904 Size: 3950 AlwNI CAG_nnn'CTG Cuts at: 1255 2822 1255 Size: 1567 2383 ApaLI G'TGCA_C Cuts at: 896 2720 896 Size: 1824 2126 ApoI r'AATT_y Cuts at: 954 3303 3559 954 Size: 2349 256 1345 Fragments arranged by size: 2349 1345 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3950 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3950 AvaI C'yCGr_G Cuts at: 585 970 585 Size: 385 3565 BamHI G'GATC_C Cuts at: 161 975 161 Size: 814 3136 BanI G'GyrC_C Cuts at: 826 966 1380 1628 1663 826 Size: 140 414 248 35 3113 Fragments arranged by size: 3113 414 248 140 35 BanII G_rGCy'C Cuts at: 601 918 964 1994 601 Size: 317 46 1030 2557 Fragments arranged by size: 2557 1030 317 46 BbeI G_GCGC'C Cuts at: 1384 1632 1384 Size: 248 3702 BbsI GAAGACnn'nnnn_ Cuts at: 525 525 Size: 3950 BciVI GTATCCnnnnn_n' Cuts at: 1848 2609 3950 1848 Size: 761 1341 1848 Fragments arranged by size: 1848 1341 761 BclI T'GATC_A Cuts at: 1470 1470 Size: 3950 BglI GCCn_nnn'nGGC Cuts at: 112 112 Size: 3950 BglII A'GATC_T Cuts at: 1465 3502 1465 Size: 2037 1913 BlpI GC'TnA_GC Cuts at: 364 1062 364 Size: 698 3252 Bme1580I G_kGCm'C Cuts at: 900 1575 1668 2724 900 Size: 675 93 1056 2126 Fragments arranged by size: 2126 1056 675 93 BmrI ACTGGGnnnn_n' Cuts at: 753 790 1162 1243 1578 3722 753 Size: 37 372 81 335 2144 981 Fragments arranged by size: 2144 981 372 335 81 37 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2317 2317 Size: 3950 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 576 902 576 Size: 326 3624 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1126 2005 2497 2795 1126 Size: 879 492 298 2281 Fragments arranged by size: 2281 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 747 3654 747 Size: 2907 1043 BsaAI yAC'GTr Cuts at: 222 1303 222 Size: 1081 2869 BsaBI GATnn'nnATC Cuts at: 6 1464 3501 6 Size: 1458 2037 455 Fragments arranged by size: 2037 1458 455 BsaHI Gr'CG_yC Cuts at: 1381 1629 2331 3827 1381 Size: 248 702 1496 1504 Fragments arranged by size: 1504 1496 702 248 BsaWI w'CCGG_w Cuts at: 1139 1660 2338 2612 2759 3170 1139 Size: 521 678 274 147 411 1919 Fragments arranged by size: 1919 678 521 411 274 147 BseYI C'CCAG_C Cuts at: 1375 2133 2710 3206 1375 Size: 758 577 496 2119 Fragments arranged by size: 2119 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 733 733 Size: 3950 BsiEI CG_ry'CG Cuts at: 104 1492 1538 2746 104 Size: 1388 46 1208 1308 Fragments arranged by size: 1388 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 370 900 918 964 1742 1932 2724 370 Size: 530 18 46 778 190 792 1596 Fragments arranged by size: 1596 792 778 530 190 46 18 Bsp1286I G_dGCh'C Cuts at: 370 601 900 918 964 1575 1668 1742 1932 Size: 231 299 18 46 611 93 74 190 Cuts at: 1932 1994 2724 370 Size: 62 730 1596 Fragments arranged by size: 1596 730 611 299 231 190 93 74 62 46 18 BspEI T'CCGG_A Cuts at: 3170 3170 Size: 3950 BspHI T'CATG_A Cuts at: 290 3126 290 Size: 2836 1114 BspMI ACCTGCnnnn'nnnn_ Cuts at: 504 1000 1214 1516 1897 504 Size: 496 214 302 381 2557 Fragments arranged by size: 2557 496 381 302 214 BsrBI CCG'CTC Cuts at: 50 2242 2296 3780 3945 50 Size: 2192 54 1484 165 55 Fragments arranged by size: 2192 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1862 1862 Size: 3950 BsrFI r'CCGG_y Cuts at: 1948 2129 2338 1948 Size: 181 209 3560 Fragments arranged by size: 3560 209 181 BssSI C'ACGA_G Cuts at: 2221 2579 2221 Size: 358 3592 BstAPI GCAn_nnn'nTGC Cuts at: 1327 1327 Size: 3950 BstBI TT'CG_AA Cuts at: 2311 2311 Size: 3950 BstEII G'GTnAC_C Cuts at: 516 516 Size: 3950 BstXI CCAn_nnnn'nTGG Cuts at: 283 283 Size: 3950 BstYI r'GATC_y Cuts at: 1 153 161 975 1442 1465 1800 2046 3058 Size: 152 8 814 467 23 335 246 1012 Cuts at: 3058 3144 3156 3502 1 Size: 86 12 346 449 Fragments arranged by size: 1012 814 467 449 346 335 246 152 86 23 12 8 Bsu36I CC'TnA_GG Cuts at: 413 413 Size: 3950 BtgI C'CryG_G Cuts at: 107 282 107 Size: 175 3775 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1900 2081 2244 1900 Size: 181 163 3606 Fragments arranged by size: 3606 181 163 BtsI GCAGTG_nn' Cuts at: 745 1193 745 Size: 448 3502 DraI TTT'AAA Cuts at: 949 949 Size: 3950 DraIII CAC_nnn'GTG Cuts at: 546 546 Size: 3950 DrdI GACnn_nn'nnGTC Cuts at: 1656 2514 1656 Size: 858 3092 EaeI y'GGCC_r Cuts at: 1535 1709 2100 2127 3472 1535 Size: 174 391 27 1345 2013 Fragments arranged by size: 2013 1345 391 174 27 EagI C'GGCC_G Cuts at: 1535 1535 Size: 3950 EarI CTCTTCn'nnn_ Cuts at: 451 457 627 1973 2183 451 Size: 6 170 1346 210 2218 Fragments arranged by size: 2218 1346 210 170 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2158 2468 2614 3760 2158 Size: 310 146 1146 2348 Fragments arranged by size: 2348 1146 310 146 EcoICRI GAG'CTC Cuts at: 916 962 916 Size: 46 3904 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 576 886 902 1775 2207 2954 3222 576 Size: 310 16 873 432 747 268 1304 Fragments arranged by size: 1304 873 747 432 310 268 16 EcoO109I rG'GnC_Cy Cuts at: 185 1089 185 Size: 904 3046 EcoRI G'AATT_C Cuts at: 954 954 Size: 3950 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1969 1969 Size: 3950 FspI TGC'GCA Cuts at: 1731 1731 Size: 3950 HaeII r_GCGC'y Cuts at: 384 1384 1632 2654 3633 384 Size: 1000 248 1022 979 701 Fragments arranged by size: 1022 1000 979 701 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1792 1822 1824 1854 1792 Size: 30 2 30 3888 Fragments arranged by size: 3888 30 30 2 HincII GTy'rAC Cuts at: 989 989 Size: 3950 KasI G'GCGC_C Cuts at: 1380 1628 1380 Size: 248 3702 KpnI G_GTAC'C Cuts at: 970 970 Size: 3950 MluI A'CGCG_T Cuts at: 3383 3383 Size: 3950 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 428 875 2621 2805 428 Size: 447 1746 184 1573 Fragments arranged by size: 1746 1573 447 184 MscI TGG'CCA Cuts at: 1711 1711 Size: 3950 MslI CAynn'nnrTG Cuts at: 817 1939 2066 817 Size: 1122 127 2701 Fragments arranged by size: 2701 1122 127 MspA1I CmG'CkG Cuts at: 723 779 1061 1375 1735 2748 2993 723 Size: 56 282 314 360 1013 245 1680 Fragments arranged by size: 1680 1013 360 314 282 245 56 NaeI GCC'GGC Cuts at: 2131 2131 Size: 3950 NarI GG'CG_CC Cuts at: 1381 1629 1381 Size: 248 3702 NcoI C'CATG_G Cuts at: 107 107 Size: 3950 NgoMIV G'CCGG_C Cuts at: 2129 2129 Size: 3950 NspI r_CATG'y Cuts at: 532 634 1003 1503 2410 532 Size: 102 369 500 907 2072 Fragments arranged by size: 2072 907 500 369 102 PciI A'CATG_T Cuts at: 528 2406 528 Size: 1878 2072 PflMI CCAn_nnn'nTGG Cuts at: 857 857 Size: 3950 PmeI GTTT'AAAC Cuts at: 949 949 Size: 3950 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3089 3121 3089 Size: 32 3918 PpuMI rG'GwC_Cy Cuts at: 185 185 Size: 3950 PstI C_TGCA'G Cuts at: 997 1682 997 Size: 685 3265 PvuI CG_AT'CG Cuts at: 104 104 Size: 3950 PvuII CAG'CTG Cuts at: 723 779 1375 1735 723 Size: 56 596 360 2938 Fragments arranged by size: 2938 596 360 56 RsrII CG'GwC_CG Cuts at: 2145 2145 Size: 3950 SacI G_AGCT'C Cuts at: 918 964 918 Size: 46 3904 SalI G'TCGA_C Cuts at: 987 987 Size: 3950 SanDI GG'GwC_CC Cuts at: 185 185 Size: 3950 SapI GCTCTTCn'nnn_ Cuts at: 451 1973 2183 451 Size: 1522 210 2218 Fragments arranged by size: 2218 1522 210 SbfI CC_TGCA'GG Cuts at: 997 997 Size: 3950 ScaI AGT'ACT Cuts at: 763 3467 763 Size: 2704 1246 SfcI C'TryA_G Cuts at: 33 993 1678 2671 2862 33 Size: 960 685 993 191 1121 Fragments arranged by size: 1121 993 960 685 191 SfoI GGC'GCC Cuts at: 1382 1630 1382 Size: 248 3702 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3950 SmaI CCC'GGG Cuts at: 587 972 587 Size: 385 3565 SmlI C'TyrA_G Cuts at: 1105 2020 2512 2774 1105 Size: 915 492 262 2281 Fragments arranged by size: 2281 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3950 SphI G_CATG'C Cuts at: 1003 1503 1003 Size: 500 3450 StyI C'CwwG_G Cuts at: 107 875 929 1084 1438 107 Size: 768 54 155 354 2619 Fragments arranged by size: 2619 768 354 155 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1533 1666 2125 2334 1533 Size: 133 459 209 3149 Fragments arranged by size: 3149 459 209 133 TatI w'GTAC_w Cuts at: 224 647 761 3465 224 Size: 423 114 2704 709 Fragments arranged by size: 2704 709 423 114 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3467 3499 3467 Size: 32 3918 Tth111I GACn'n_nGTC Cuts at: 1747 1747 Size: 3950 XbaI T'CTAG_A Cuts at: 981 981 Size: 3950 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1445 1445 Size: 3950 XmaI C'CCGG_G Cuts at: 585 970 585 Size: 385 3565 XmnI GAAnn'nnTTC Cuts at: 271 271 Size: 3950 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AhdI AleI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI