(Circular) (Six-base) MAPSORT of: pF1KE2657.seq Check: 8681 from: 1 to: 3704 pF1KE2657 3704 bp With 182 enzymes: SgfI * December 21, 2016 11:09 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 472 472 Size: 3704 AccI GT'mk_AC Cuts at: 742 742 Size: 3704 Acc65I G'GTAC_C Cuts at: 720 720 Size: 3704 AclI AA'CG_TT Cuts at: 3544 3544 Size: 3704 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1529 1961 2708 2976 1529 Size: 432 747 268 2257 Fragments arranged by size: 2257 747 432 268 AflIII A'CryG_T Cuts at: 1056 2160 3137 1056 Size: 1104 977 1623 Fragments arranged by size: 1623 1104 977 AgeI A'CCGG_T Cuts at: 2092 2092 Size: 3704 AlwNI CAG_nnn'CTG Cuts at: 432 435 457 1009 2576 432 Size: 3 22 552 1567 1560 Fragments arranged by size: 1567 1560 552 22 3 ApaLI G'TGCA_C Cuts at: 199 2474 199 Size: 2275 1429 ApoI r'AATT_y Cuts at: 708 3057 3313 708 Size: 2349 256 1099 Fragments arranged by size: 2349 1099 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3704 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3704 AvaI C'yCGr_G Cuts at: 167 724 167 Size: 557 3147 BamHI G'GATC_C Cuts at: 729 729 Size: 3704 BanI G'GyrC_C Cuts at: 330 460 681 720 1134 1382 1417 330 Size: 130 221 39 414 248 35 2617 Fragments arranged by size: 2617 414 248 221 130 39 35 BanII G_rGCy'C Cuts at: 145 267 718 1748 145 Size: 122 451 1030 2101 Fragments arranged by size: 2101 1030 451 122 BbeI G_GCGC'C Cuts at: 334 1138 1386 334 Size: 804 248 2652 Fragments arranged by size: 2652 804 248 BciVI GTATCCnnnnn_n' Cuts at: 1602 2363 3704 1602 Size: 761 1341 1602 Fragments arranged by size: 1602 1341 761 BclI T'GATC_A Cuts at: 1224 1224 Size: 3704 BglI GCCn_nnn'nGGC Cuts at: 179 179 Size: 3704 BglII A'GATC_T Cuts at: 1219 3256 1219 Size: 2037 1667 BlpI GC'TnA_GC Cuts at: 618 816 618 Size: 198 3506 Bme1580I G_kGCm'C Cuts at: 203 1329 1422 2478 203 Size: 1126 93 1056 1429 Fragments arranged by size: 1429 1126 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 190 497 916 997 1332 3476 190 Size: 307 419 81 335 2144 418 Fragments arranged by size: 2144 419 418 335 307 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 286 2071 286 Size: 1785 1919 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 334 473 334 Size: 139 3565 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 529 880 1759 2251 2549 529 Size: 351 879 492 298 1684 Fragments arranged by size: 1684 879 492 351 298 BsaI GGTCTCn'nnnn_ Cuts at: 283 3408 283 Size: 3125 579 BsaAI yAC'GTr Cuts at: 1057 1057 Size: 3704 BsaBI GATnn'nnATC Cuts at: 6 1218 3255 6 Size: 1212 2037 455 Fragments arranged by size: 2037 1212 455 BsaHI Gr'CG_yC Cuts at: 331 1135 1383 2085 3581 331 Size: 804 248 702 1496 454 Fragments arranged by size: 1496 804 702 454 248 BsaWI w'CCGG_w Cuts at: 628 893 1414 2092 2366 2513 2924 628 Size: 265 521 678 274 147 411 1408 Fragments arranged by size: 1408 678 521 411 274 265 147 BseYI C'CCAG_C Cuts at: 341 588 1129 1887 2464 2960 341 Size: 247 541 758 577 496 1085 Fragments arranged by size: 1085 758 577 541 496 247 BsiEI CG_ry'CG Cuts at: 104 1246 1292 2500 104 Size: 1142 46 1208 1308 Fragments arranged by size: 1308 1208 1142 46 BsiHKAI G_wGCw'C Cuts at: 203 368 718 1496 1686 2478 203 Size: 165 350 778 190 792 1429 Fragments arranged by size: 1429 792 778 350 190 165 BsmI GAATG_Cn' Cuts at: 182 553 182 Size: 371 3333 Bsp1286I G_dGCh'C Cuts at: 145 203 267 368 718 1329 1422 1496 1686 Size: 58 64 101 350 611 93 74 190 Cuts at: 1686 1748 2478 145 Size: 62 730 1371 Fragments arranged by size: 1371 730 611 350 190 101 93 74 64 62 58 BspEI T'CCGG_A Cuts at: 628 2924 628 Size: 2296 1408 BspHI T'CATG_A Cuts at: 476 2880 476 Size: 2404 1300 BspMI ACCTGCnnnn'nnnn_ Cuts at: 472 754 968 1270 1651 472 Size: 282 214 302 381 2525 Fragments arranged by size: 2525 381 302 282 214 BsrBI CCG'CTC Cuts at: 50 687 1996 2050 3534 3699 50 Size: 637 1309 54 1484 165 55 Fragments arranged by size: 1484 1309 637 165 55 54 BsrDI GCAATG_nn' Cuts at: 1616 1616 Size: 3704 BsrFI r'CCGG_y Cuts at: 1702 1883 2092 1702 Size: 181 209 3314 Fragments arranged by size: 3314 209 181 BssSI C'ACGA_G Cuts at: 409 1975 2333 409 Size: 1566 358 1780 Fragments arranged by size: 1780 1566 358 BstAPI GCAn_nnn'nTGC Cuts at: 188 467 1081 188 Size: 279 614 2811 Fragments arranged by size: 2811 614 279 BstBI TT'CG_AA Cuts at: 2065 2065 Size: 3704 BstEII G'GTnAC_C Cuts at: 372 372 Size: 3704 BstXI CCAn_nnnn'nTGG Cuts at: 114 114 Size: 3704 BstYI r'GATC_y Cuts at: 1 729 1196 1219 1554 1800 2812 2898 2910 Size: 728 467 23 335 246 1012 86 12 Cuts at: 2910 3256 1 Size: 346 449 Fragments arranged by size: 1012 728 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 3704 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1654 1835 1998 1654 Size: 181 163 3360 Fragments arranged by size: 3360 181 163 BtsI GCAGTG_nn' Cuts at: 377 578 947 377 Size: 201 369 3134 Fragments arranged by size: 3134 369 201 DraI TTT'AAA Cuts at: 703 703 Size: 3704 DraIII CAC_nnn'GTG Cuts at: 671 671 Size: 3704 DrdI GACnn_nn'nnGTC Cuts at: 1410 2268 1410 Size: 858 2846 EaeI y'GGCC_r Cuts at: 656 1289 1463 1854 1881 3226 656 Size: 633 174 391 27 1345 1134 Fragments arranged by size: 1345 1134 633 391 174 27 EagI C'GGCC_G Cuts at: 1289 1289 Size: 3704 EarI CTCTTCn'nnn_ Cuts at: 1727 1937 1727 Size: 210 3494 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1912 2222 2368 3514 1912 Size: 310 146 1146 2102 Fragments arranged by size: 2102 1146 310 146 EcoICRI GAG'CTC Cuts at: 716 716 Size: 3704 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 334 473 1529 1961 2708 2976 334 Size: 139 1056 432 747 268 1062 Fragments arranged by size: 1062 1056 747 432 268 139 EcoO109I rG'GnC_Cy Cuts at: 129 636 843 129 Size: 507 207 2990 Fragments arranged by size: 2990 507 207 EcoRI G'AATT_C Cuts at: 708 708 Size: 3704 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1723 1723 Size: 3704 FspI TGC'GCA Cuts at: 1485 1485 Size: 3704 HaeII r_GCGC'y Cuts at: 334 1138 1386 2408 3387 334 Size: 804 248 1022 979 651 Fragments arranged by size: 1022 979 804 651 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 601 633 1546 1576 1578 1608 601 Size: 32 913 30 2 30 2697 Fragments arranged by size: 2697 913 32 30 30 2 HincII GTy'rAC Cuts at: 743 743 Size: 3704 KasI G'GCGC_C Cuts at: 330 1134 1382 330 Size: 804 248 2652 Fragments arranged by size: 2652 804 248 KpnI G_GTAC'C Cuts at: 724 724 Size: 3704 MluI A'CGCG_T Cuts at: 3137 3137 Size: 3704 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2375 2559 2375 Size: 184 3520 MscI TGG'CCA Cuts at: 1465 1465 Size: 3704 MslI CAynn'nnrTG Cuts at: 112 247 304 658 1693 1820 112 Size: 135 57 354 1035 127 1996 Fragments arranged by size: 1996 1035 354 135 127 57 MspA1I CmG'CkG Cuts at: 432 815 1129 1489 2502 2747 432 Size: 383 314 360 1013 245 1389 Fragments arranged by size: 1389 1013 383 360 314 245 NaeI GCC'GGC Cuts at: 1885 1885 Size: 3704 NarI GG'CG_CC Cuts at: 331 1135 1383 331 Size: 804 248 2652 Fragments arranged by size: 2652 804 248 NcoI C'CATG_G Cuts at: 107 107 Size: 3704 NgoMIV G'CCGG_C Cuts at: 1883 1883 Size: 3704 NspI r_CATG'y Cuts at: 757 1257 2164 757 Size: 500 907 2297 Fragments arranged by size: 2297 907 500 PciI A'CATG_T Cuts at: 2160 2160 Size: 3704 PflMI CCAn_nnn'nTGG Cuts at: 457 457 Size: 3704 PmeI GTTT'AAAC Cuts at: 703 703 Size: 3704 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2843 2875 2843 Size: 32 3672 PpuMI rG'GwC_Cy Cuts at: 129 636 129 Size: 507 3197 PstI C_TGCA'G Cuts at: 560 751 1436 560 Size: 191 685 2828 Fragments arranged by size: 2828 685 191 PvuI CG_AT'CG Cuts at: 104 104 Size: 3704 PvuII CAG'CTG Cuts at: 432 1129 1489 432 Size: 697 360 2647 Fragments arranged by size: 2647 697 360 RsrII CG'GwC_CG Cuts at: 1899 1899 Size: 3704 SacI G_AGCT'C Cuts at: 718 718 Size: 3704 SalI G'TCGA_C Cuts at: 741 741 Size: 3704 SanDI GG'GwC_CC Cuts at: 636 636 Size: 3704 SapI GCTCTTCn'nnn_ Cuts at: 1727 1937 1727 Size: 210 3494 SbfI CC_TGCA'GG Cuts at: 751 751 Size: 3704 ScaI AGT'ACT Cuts at: 3221 3221 Size: 3704 SexAI A'CCwGG_T Cuts at: 368 368 Size: 3704 SfcI C'TryA_G Cuts at: 33 556 747 1432 2425 2616 33 Size: 523 191 685 993 191 1121 Fragments arranged by size: 1121 993 685 523 191 191 SfoI GGC'GCC Cuts at: 332 1136 1384 332 Size: 804 248 2652 Fragments arranged by size: 2652 804 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3704 SmaI CCC'GGG Cuts at: 169 726 169 Size: 557 3147 SmlI C'TyrA_G Cuts at: 508 859 1774 2266 2528 508 Size: 351 915 492 262 1684 Fragments arranged by size: 1684 915 492 351 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3704 SphI G_CATG'C Cuts at: 757 1257 757 Size: 500 3204 StyI C'CwwG_G Cuts at: 107 838 1192 107 Size: 731 354 2619 Fragments arranged by size: 2619 731 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1287 1420 1879 2088 1287 Size: 133 459 209 2903 Fragments arranged by size: 2903 459 209 133 TatI w'GTAC_w Cuts at: 3219 3219 Size: 3704 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3221 3253 3221 Size: 32 3672 Tth111I GACn'n_nGTC Cuts at: 1501 1501 Size: 3704 XbaI T'CTAG_A Cuts at: 735 735 Size: 3704 XcmI CCAnnnn_n'nnnnTGG Cuts at: 408 1199 408 Size: 791 2913 XmaI C'CCGG_G Cuts at: 167 724 167 Size: 557 3147 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI